CSPI01G25960 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G25960
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptioncalmodulin-binding protein 60 B-like isoform X1
LocationChr1: 21343780 .. 21345902 (+)
RNA-Seq ExpressionCSPI01G25960
SyntenyCSPI01G25960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATACATACTACTTTCTTCCTGATCTTCATATACATACCTTGTTGTTTCAATTATAAAGACATCAGCCATGGACTACCTAAATACAAAAACCTTCTTCCGTCCTGATCAAAATTATCAGACAACCTCCTCCCACTTCTCTTTTCCATTTGGGTATGTCTTTCTACTATATGTTTCATCATAATCATATCCATCCTCTCTATTTTGTTGGGTTATATATATATTTCTCAGGTTTGGGGTTGGAGATAATCATGAGGTTTTGTCTCCTAATTCTGTGGCTTATGTTGAGAATTACTTTCGCCCCATGGTATGTTACCCCATTTTTCAAATTTGATTGACGTGAAAGCTTAATTAATTAAGCTTATTAATGTGTGATGGTAAAATTAATTTCAGATACGAGAGGAAGTGGAGGCAAAGATTAATGCTCGCTTTAACGTCTCTACCAATTACCAATCATGTTCTAATTCAAGTACTAGTAGGGAAGGAGTACTTGGTGAAGATCGTGCAGTTGTTATTGTTGGTGGAGGCCGATTGAGAACGGAGAATCAACAACAACTGAAGTTACGTTTCACGAACAAGGTTTCATCCACCGTCTTCACCGTAAATGACATCGAAGCCGAGAATGGGGAGGAGCTGCGTGTTGAATTATTTGATGCTGTCAATGATCGCATCATTGACGCTACTCATCCTTTGTCGTCGGCGTCGATTGAGGTTGTTGTCCTCGATGGAGAATTCAACGACGGGGAAGCTATAACTCAATCGGATTTCAACAGAAGTGTTGTGCCAGAAAGGCTGGGAGAACGACCTTTGTTGGTTGGTCGTGATAAGAGGTTTCGTTTAGAAAAGGGAGTTTATTCCATCACCGATTTGTCATTCACTAGAAACTCAAGCAGGAGCAGGACTAAGAAGATTTGTTTGGGACTAAGGGTTACACAGGACTCCAACAACAACTATCCCACAATTGGACACACCGTGTCTAATCCTTTTAGAGTGAAGGATCACCGAGGCCAACGTATGTATCTTGAGTCATCATTCATTCGCTAGAAAGCTTAATTGCATCCATCTTTCCATTTTTATTTACAATAATAAATATATTGCTTTATATGGGCATATCTAACAGTGAACAAGAAACACCATCCTCCAAAAGGAGAAGATGAAGTATGGAGATTGGAAGGGATTGGGCGAAATGGCGAATATCACAAGCGTCTAACTTCCCATACTATCCTAAATGTTGATGACTTTTTGAAGGCGTATCAAAAGGACTCTCGTTCTCTAAGAAAGGTATAATATATATCTCTAATGTGGTTTAATAATAAGTCAATATTGTAGTAATTAAAACTATATATATATAATTGAAATATATAATTAATTTTGGTAATTTTGCATGCAGTGGTTAGGAAATAGAGTTTCAGAGAAGAAATGGAAGTCAATGGTAAAGCATGCTGAAGAATATGTTCCTATCACCAACGCCATCGTCCCCACTTTTGATCCACCCACCGGCTTTCAGGTCTCTTTAATTCTCATCTATCACATCTACATTTTTAAATGCCTCTTTTCTTTCTTTCTTAGAAATATTTTTGAAAAGTTATTACTACATATTTCTTTTCATATCTTTATAAGTTGGGGTTTTCAAACTGAGAATTGACAATATATTATATGAAAAAAGCAGCAAAATTTGGTGGAAAATGAAGCCATGGGAGGTGTAGAAGAAGTTAGCAACCAAAACAACATTGGCGATGGGTTCCAAGATCATACATCATTATCGGCTGATGTTATTCAAGATCATTTTTATCGAATATTTCAAGGTTTTGATCTTCCTTTTTTTAAAAAAAAAAAAAAAAAAATCAACTCTCTCTCTTGAGTTTTTTTTTTTAATATCTATATTATTATTATTGTGAAAAAGATAACTATATAATGTTGTGTGTTTTGATTAATTTGTAGATTACCCAATACAAACATTGTTGTCACAAGGAGAAGCTTCAACCTCAAATGGCCTATATAATTATGTTGATCAACTTAGTACAAGTACATGCAACCAATCCTTTCAAGGAGCTGCCTTCAATAAATTGGTGCCTAAATCTAAATGA

mRNA sequence

ATACATACTACTTTCTTCCTGATCTTCATATACATACCTTGTTGTTTCAATTATAAAGACATCAGCCATGGACTACCTAAATACAAAAACCTTCTTCCGTCCTGATCAAAATTATCAGACAACCTCCTCCCACTTCTCTTTTCCATTTGGGTTTGGGGTTGGAGATAATCATGAGGTTTTGTCTCCTAATTCTGTGGCTTATGTTGAGAATTACTTTCGCCCCATGATACGAGAGGAAGTGGAGGCAAAGATTAATGCTCGCTTTAACGTCTCTACCAATTACCAATCATGTTCTAATTCAAGTACTAGTAGGGAAGGAGTACTTGGTGAAGATCGTGCAGTTGTTATTGTTGGTGGAGGCCGATTGAGAACGGAGAATCAACAACAACTGAAGTTACGTTTCACGAACAAGGTTTCATCCACCGTCTTCACCGTAAATGACATCGAAGCCGAGAATGGGGAGGAGCTGCGTGTTGAATTATTTGATGCTGTCAATGATCGCATCATTGACGCTACTCATCCTTTGTCGTCGGCGTCGATTGAGGTTGTTGTCCTCGATGGAGAATTCAACGACGGGGAAGCTATAACTCAATCGGATTTCAACAGAAGTGTTGTGCCAGAAAGGCTGGGAGAACGACCTTTGTTGGTTGGTCGTGATAAGAGGTTTCGTTTAGAAAAGGGAGTTTATTCCATCACCGATTTGTCATTCACTAGAAACTCAAGCAGGAGCAGGACTAAGAAGATTTGTTTGGGACTAAGGGTTACACAGGACTCCAACAACAACTATCCCACAATTGGACACACCGTGTCTAATCCTTTTAGAGTGAAGGATCACCGAGGCCAACTGAACAAGAAACACCATCCTCCAAAAGGAGAAGATGAAGTATGGAGATTGGAAGGGATTGGGCGAAATGGCGAATATCACAAGCGTCTAACTTCCCATACTATCCTAAATGTTGATGACTTTTTGAAGGCGTATCAAAAGGACTCTCGTTCTCTAAGAAAGTGGTTAGGAAATAGAGTTTCAGAGAAGAAATGGAAGTCAATGGTAAAGCATGCTGAAGAATATGTTCCTATCACCAACGCCATCGTCCCCACTTTTGATCCACCCACCGGCTTTCAGCAAAATTTGGTGGAAAATGAAGCCATGGGAGGTGTAGAAGAAGTTAGCAACCAAAACAACATTGGCGATGGGTTCCAAGATCATACATCATTATCGGCTGATGTTATTCAAGATCATTTTTATCGAATATTTCAAGATTACCCAATACAAACATTGTTGTCACAAGGAGAAGCTTCAACCTCAAATGGCCTATATAATTATGTTGATCAACTTAGTACAAGTACATGCAACCAATCCTTTCAAGGAGCTGCCTTCAATAAATTGGTGCCTAAATCTAAATGA

Coding sequence (CDS)

ATGGACTACCTAAATACAAAAACCTTCTTCCGTCCTGATCAAAATTATCAGACAACCTCCTCCCACTTCTCTTTTCCATTTGGGTTTGGGGTTGGAGATAATCATGAGGTTTTGTCTCCTAATTCTGTGGCTTATGTTGAGAATTACTTTCGCCCCATGATACGAGAGGAAGTGGAGGCAAAGATTAATGCTCGCTTTAACGTCTCTACCAATTACCAATCATGTTCTAATTCAAGTACTAGTAGGGAAGGAGTACTTGGTGAAGATCGTGCAGTTGTTATTGTTGGTGGAGGCCGATTGAGAACGGAGAATCAACAACAACTGAAGTTACGTTTCACGAACAAGGTTTCATCCACCGTCTTCACCGTAAATGACATCGAAGCCGAGAATGGGGAGGAGCTGCGTGTTGAATTATTTGATGCTGTCAATGATCGCATCATTGACGCTACTCATCCTTTGTCGTCGGCGTCGATTGAGGTTGTTGTCCTCGATGGAGAATTCAACGACGGGGAAGCTATAACTCAATCGGATTTCAACAGAAGTGTTGTGCCAGAAAGGCTGGGAGAACGACCTTTGTTGGTTGGTCGTGATAAGAGGTTTCGTTTAGAAAAGGGAGTTTATTCCATCACCGATTTGTCATTCACTAGAAACTCAAGCAGGAGCAGGACTAAGAAGATTTGTTTGGGACTAAGGGTTACACAGGACTCCAACAACAACTATCCCACAATTGGACACACCGTGTCTAATCCTTTTAGAGTGAAGGATCACCGAGGCCAACTGAACAAGAAACACCATCCTCCAAAAGGAGAAGATGAAGTATGGAGATTGGAAGGGATTGGGCGAAATGGCGAATATCACAAGCGTCTAACTTCCCATACTATCCTAAATGTTGATGACTTTTTGAAGGCGTATCAAAAGGACTCTCGTTCTCTAAGAAAGTGGTTAGGAAATAGAGTTTCAGAGAAGAAATGGAAGTCAATGGTAAAGCATGCTGAAGAATATGTTCCTATCACCAACGCCATCGTCCCCACTTTTGATCCACCCACCGGCTTTCAGCAAAATTTGGTGGAAAATGAAGCCATGGGAGGTGTAGAAGAAGTTAGCAACCAAAACAACATTGGCGATGGGTTCCAAGATCATACATCATTATCGGCTGATGTTATTCAAGATCATTTTTATCGAATATTTCAAGATTACCCAATACAAACATTGTTGTCACAAGGAGAAGCTTCAACCTCAAATGGCCTATATAATTATGTTGATCAACTTAGTACAAGTACATGCAACCAATCCTTTCAAGGAGCTGCCTTCAATAAATTGGTGCCTAAATCTAAATGA

Protein sequence

MDYLNTKTFFRPDQNYQTTSSHFSFPFGFGVGDNHEVLSPNSVAYVENYFRPMIREEVEAKINARFNVSTNYQSCSNSSTSREGVLGEDRAVVIVGGGRLRTENQQQLKLRFTNKVSSTVFTVNDIEAENGEELRVELFDAVNDRIIDATHPLSSASIEVVVLDGEFNDGEAITQSDFNRSVVPERLGERPLLVGRDKRFRLEKGVYSITDLSFTRNSSRSRTKKICLGLRVTQDSNNNYPTIGHTVSNPFRVKDHRGQLNKKHHPPKGEDEVWRLEGIGRNGEYHKRLTSHTILNVDDFLKAYQKDSRSLRKWLGNRVSEKKWKSMVKHAEEYVPITNAIVPTFDPPTGFQQNLVENEAMGGVEEVSNQNNIGDGFQDHTSLSADVIQDHFYRIFQDYPIQTLLSQGEASTSNGLYNYVDQLSTSTCNQSFQGAAFNKLVPKSK*
Homology
BLAST of CSPI01G25960 vs. ExPASy Swiss-Prot
Match: Q9FKL6 (Calmodulin-binding protein 60 B OS=Arabidopsis thaliana OX=3702 GN=CBP60B PE=2 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 1.1e-40
Identity = 100/239 (41.84%), Postives = 146/239 (61.09%), Query Frame = 0

Query: 99  RLRTENQQQLKLRFTNKVSSTVFTVNDIEAENGEELRVELFDAVNDRIIDATHPLSSASI 158
           R+   + ++L+L F +++S  +FT   +E E G  + V L DA   R +    P +SA +
Sbjct: 85  RIEGPDGRKLQLHFKSRLSLPLFTGGKVEGEQGAVIHVVLIDANTGRAV-VYGPEASAKL 144

Query: 159 EVVVLDGEFN--DGEAITQSDFNRSVVPERLGERPLLVGRDKRFRLEKGVYSITDLSFTR 218
            +VVL+G+FN  D E  TQ +F   VV ER G+RPLL G +    L++GV ++ +L FT 
Sbjct: 145 HIVVLEGDFNTEDDEDWTQEEFESHVVKERSGKRPLLTG-EVYVTLKEGVGTLGELVFTD 204

Query: 219 NSSRSRTKKICLGLRVTQDSNNNYPTIGHTVSNPFRVKDHRGQLNKKHHPPKGEDEVWRL 278
           NSS  R++K  LGLRV     +    I    +  F VKDHRG+L KKH+PP   D+VWRL
Sbjct: 205 NSSWIRSRKFRLGLRVVSGCCDGM-RIREAKTEAFVVKDHRGELYKKHYPPALNDDVWRL 264

Query: 279 EGIGRNGEYHKRLTSHTILNVDDFLKAYQKDSRSLRKWLGNRVSEKKWKSMVKHAEEYV 336
           + IG++G +HK+LT+  I  V+DFL+   KDS  LR  LG+ +S K W ++V+HA+  V
Sbjct: 265 DKIGKDGAFHKKLTAEGINTVEDFLRVMVKDSPKLRTILGSGMSNKMWDALVEHAKTCV 320

BLAST of CSPI01G25960 vs. ExPASy Swiss-Prot
Match: C0SV51 (Calmodulin-binding protein 60 C OS=Arabidopsis thaliana OX=3702 GN=CBP60C PE=2 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 1.3e-39
Identity = 103/274 (37.59%), Postives = 157/274 (57.30%), Query Frame = 0

Query: 75  CSNSSTSREGVLGE--DRAVVIVGGGRLRTENQ---------QQLKLRFTNKVSSTVFTV 134
           CS+       V+ E  +RA+  +G  RL   +          + L+L+F +++S  +FT 
Sbjct: 54  CSSLEPILRRVVSEEVERALAKLGPARLSERSSPKRIEGIGGRNLQLQFRSRLSVPLFTG 113

Query: 135 NDIEAENGEELRVELFDAVNDRIIDATHPLSSASIEVVVLDGEFN--DGEAITQSDFNRS 194
             IE E G  + V L D     ++    P +SA ++VVVLDG+FN  D +  +  +F   
Sbjct: 114 GKIEGEQGAAIHVVLLDMTTGHVL-TVGPEASAKLDVVVLDGDFNTEDDDGWSGEEFEGH 173

Query: 195 VVPERLGERPLLVGRDKRFRLEKGVYSITDLSFTRNSSRSRTKKICLGLRVTQDSNNNYP 254
           +V ER G+RPLL G D +  L++GV ++ +L FT NSS  R +K  LGLRV+        
Sbjct: 174 LVKERQGKRPLLTG-DVQVTLKEGVGTLGELIFTDNSSWIRCRKFRLGLRVSSGYCEGM- 233

Query: 255 TIGHTVSNPFRVKDHRGQLNKKHHPPKGEDEVWRLEGIGRNGEYHKRLTSHTILNVDDFL 314
            +    +  F VKDHRG+L KKH+PP  +DEVWRLE IG++G +HK+L    I NV +FL
Sbjct: 234 RVREAKTEAFTVKDHRGELYKKHYPPALDDEVWRLEKIGKDGAFHKKLNKAGIYNVKEFL 293

Query: 315 KAYQKDSRSLRKWLGNRVSEKKWKSMVKHAEEYV 336
           +   KDS+ LR  LG+ +S + W+++ +H++  V
Sbjct: 294 RLMVKDSQKLRTILGSGMSNRMWETLAEHSKTCV 324

BLAST of CSPI01G25960 vs. ExPASy Swiss-Prot
Match: Q0WVV6 (Calmodulin-binding protein 60 D OS=Arabidopsis thaliana OX=3702 GN=CBP60D PE=2 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 1.3e-39
Identity = 102/239 (42.68%), Postives = 143/239 (59.83%), Query Frame = 0

Query: 99  RLRTENQQQLKLRFTNKVSSTVFTVNDIEAENGEELRVELFDAVNDRIIDATHPLSSASI 158
           R+   + + L+L F +++S  +FT   +E E G  + V L DA   R +    P +S  +
Sbjct: 74  RIGGPDGRNLQLHFKSRLSLPLFTGGRVEGEQGATIHVVLIDANTGRPV-TVGPEASLKL 133

Query: 159 EVVVLDGEFN--DGEAITQSDFNRSVVPERLGERPLLVGRDKRFRLEKGVYSITDLSFTR 218
           EVVVL G+FN  D E  TQ +F   VV ER G+RPLL G D    L++GV ++ ++ FT 
Sbjct: 134 EVVVLGGDFNNEDDEDWTQEEFESHVVKEREGKRPLLTG-DLFVVLKEGVGTLGEIVFTD 193

Query: 219 NSSRSRTKKICLGLRVTQDSNNNYPTIGHTVSNPFRVKDHRGQLNKKHHPPKGEDEVWRL 278
           NSS  R++K  LGLRV     +    I    +  F VKDHRG+L KKH+PP   DEVWRL
Sbjct: 194 NSSWIRSRKFRLGLRVPSGYCDGI-RIREAKTEAFSVKDHRGELYKKHYPPALNDEVWRL 253

Query: 279 EGIGRNGEYHKRLTSHTILNVDDFLKAYQKDSRSLRKWLGNRVSEKKWKSMVKHAEEYV 336
           E IG++G +HKRLT+  I+ V+ FL+   +DS  LR  LG+ +S K W  +V+HA+  V
Sbjct: 254 EKIGKDGAFHKRLTAAGIVTVEGFLRQLVRDSTKLRAILGSGMSNKMWDLLVEHAKTCV 309

BLAST of CSPI01G25960 vs. ExPASy Swiss-Prot
Match: C0SVV6 (Calmodulin-binding protein 60 A OS=Arabidopsis thaliana OX=3702 GN=CBP60A PE=2 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 3.7e-39
Identity = 98/248 (39.52%), Postives = 144/248 (58.06%), Query Frame = 0

Query: 106 QQLKLRFTNKVSSTVFTVNDIEAENGEELRVELFDAVNDRIIDATHPLSSASIEVVVLDG 165
           + L+L+F N +S  VFT   IE + G+ +RV L D    +I  ++ P SSA +EV V++G
Sbjct: 69  RNLQLKFLNNLSLPVFTSARIEGDEGQAIRVGLIDPSTGQIF-SSGPASSAKLEVFVVEG 128

Query: 166 EFNDGEAITQSDFNRSVVPERLGERPLLVGRDKRFRLEKGVYSITDLSFTRNSSRSRTKK 225
           +FN     T  D   ++V ER G++PLL G      L  G+  + ++SFT NSS +R++K
Sbjct: 129 DFNSVSDWTDEDIRNNIVREREGKKPLLNGNVFAV-LNDGIGVMDEISFTDNSSWTRSRK 188

Query: 226 ICLGLRVTQDSNNNYPTIGHTVSNPFRVKDHRGQLNKKHHPPKGEDEVWRLEGIGRNGEY 285
             LG+R+      +Y  I   ++  F V+DHRG+L KKHHPP   DEVWRLE IG++G +
Sbjct: 189 FRLGVRIVDQF--DYVKIREAITESFVVRDHRGELYKKHHPPSLFDEVWRLEKIGKDGAF 248

Query: 286 HKRLTSHTILNVDDFLKAYQKDSRSLRKWLGNRVSEKKWKSMVKHAEEYVPITNAIVPTF 345
           H+RL    I  V DFL  +  +S  LR+ LG  +S K W+  + HA   V   ++ V  +
Sbjct: 249 HRRLNLSNINTVKDFLTHFHLNSSKLRQVLGTGMSSKMWEITLDHARSCV--LDSSVHVY 308

Query: 346 DPPTGFQQ 354
             P GFQ+
Sbjct: 309 QAP-GFQK 309

BLAST of CSPI01G25960 vs. ExPASy Swiss-Prot
Match: F4IPM3 (Calmodulin-binding protein 60 E OS=Arabidopsis thaliana OX=3702 GN=CBP60E PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.8e-38
Identity = 101/292 (34.59%), Postives = 158/292 (54.11%), Query Frame = 0

Query: 46  VENYFRPMIREEVEAKINARFNVSTNYQSCSNSSTSREGVLGEDRAVVIVGGGRLRTENQ 105
           +E  FR ++ EEVE  ++   N     +S                        R++  N 
Sbjct: 47  LEPLFRRIVSEEVERALSRLGNAKLTSRSPEPK--------------------RIQDRNG 106

Query: 106 QQLKLRFTNKVSSTVFTVNDIEAENGEELRVELFDAVNDRIIDATHPLSSASIEVVVLDG 165
           + L+L F  ++   +FT   +E E G  + V L DA    ++  T   S++ + VVVL+G
Sbjct: 107 RNLQLHFRTRMPPHLFTGGKVEGERGSAIHVVLIDANTGNVVQ-TGEESASKLNVVVLEG 166

Query: 166 EFN--DGEAITQSDFNRSVVPERLGERPLLVGRDKRFRLEKGVYSITDLSFTRNSSRSRT 225
           +FN  D E  T+  F    V ER G+RP+L G D +  L++GV ++ +L+FT NSS  R+
Sbjct: 167 DFNDEDDEDWTREHFESFEVKEREGKRPILTG-DTQIVLKEGVGTLGELTFTDNSSWIRS 226

Query: 226 KKICLGLRVTQDSNNNYPTIGHTVSNPFRVKDHRGQLNKKHHPPKGEDEVWRLEGIGRNG 285
           +K  LG++      +++  I    + PF VKDHRG+L KKH+PP   DEVWRL+ I ++G
Sbjct: 227 RKFRLGVKPASGYGDSF-CIREAKTEPFAVKDHRGELYKKHYPPAVHDEVWRLDRIAKDG 286

Query: 286 EYHKRLTSHTILNVDDFLKAYQKDSRSLRKWLGNRVSEKKWKSMVKHAEEYV 336
             HK+L    I+ V+DFL+   KD + LR  LG+ +S + W++ V+HA+  V
Sbjct: 287 VLHKKLLKANIVTVEDFLRLLVKDPQKLRNLLGSGMSNRMWENTVEHAKTCV 315

BLAST of CSPI01G25960 vs. ExPASy TrEMBL
Match: A0A1S3BPF4 (calmodulin-binding protein 60 B-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492121 PE=3 SV=1)

HSP 1 Score: 744.6 bits (1921), Expect = 2.4e-211
Identity = 381/435 (87.59%), Postives = 397/435 (91.26%), Query Frame = 0

Query: 7   KTFFRPDQNYQTTSSHFSFPFGFGVGDNHEVLSPNSVAYVENYFRPMIREEVEAKINARF 66
           KTFF P QNYQTTSSH+SFP GFGVGD+H VLSP SVAYVENYFRP+IR+EVEAKINA F
Sbjct: 3   KTFFCP-QNYQTTSSHYSFPSGFGVGDSHLVLSPKSVAYVENYFRPLIRKEVEAKINAHF 62

Query: 67  NVSTNYQSCSNSSTSREGVLGEDRAVVIVGGGRLRTENQQQLKLRFTNKVSSTVFTVNDI 126
           NVST+ QSCSNSSTSREGVLGEDRAVVIVGGGRLR ENQQQLKLRFTNKV ST++TVND+
Sbjct: 63  NVSTD-QSCSNSSTSREGVLGEDRAVVIVGGGRLRKENQQQLKLRFTNKVPSTIYTVNDV 122

Query: 127 EAENGEELRVELFDAVNDRIIDATHPLSSASIEVVVLDGEFNDGEAITQSDFNRSVVPER 186
           EAENGEELRVELFD VNDRIID THPLSSA IEVVVLD EFND EAITQSDFNRSVVP+R
Sbjct: 123 EAENGEELRVELFDVVNDRIIDVTHPLSSARIEVVVLDREFNDEEAITQSDFNRSVVPQR 182

Query: 187 LGERPLLVGRDKRFRLEKGVYSITDLSFTRNSSRSRTKKICLGLRVTQDS-NNNYPTIGH 246
           LGERPLLVG  KRFRLE GVYSITDLSFTRNSSR RTKKI LGLRV  DS NNNYPTIGH
Sbjct: 183 LGERPLLVGGHKRFRLENGVYSITDLSFTRNSSRCRTKKIRLGLRVINDSNNNNYPTIGH 242

Query: 247 TVSNPFRVKDHRGQLNKKHHPPKGEDEVWRLEGIGRNGEYHKRLTSHTILNVDDFLKAYQ 306
            VSNPFRVKDHRGQLN+K HPPKGEDEVWRLEGIGRNGEYHKRLTSH IL VDDFLKAYQ
Sbjct: 243 AVSNPFRVKDHRGQLNQKRHPPKGEDEVWRLEGIGRNGEYHKRLTSHGILYVDDFLKAYQ 302

Query: 307 KDSRSLRKWLGNRVSEKKWKSMVKHAEEYVPITNAIVPTFDPPTGFQQNLVENEAMGGVE 366
           KDSRSLRKWLGNRVS+KKWKSMVKHA EYVPITN IVPTFDPPT FQQNLVEN+AMGGVE
Sbjct: 303 KDSRSLRKWLGNRVSDKKWKSMVKHAHEYVPITNTIVPTFDPPTSFQQNLVENKAMGGVE 362

Query: 367 EVSNQNNIGDGFQDHTSLSADVIQDHFYRIFQDYPIQTLLSQGEASTSNGLYNYVDQLST 426
           EVSNQNNIGDGFQ+HTSL ADVIQDHF+RIFQD+P QTLLSQGEASTSNG YNYV+  ST
Sbjct: 363 EVSNQNNIGDGFQEHTSLLADVIQDHFHRIFQDFPTQTLLSQGEASTSNGYYNYVEH-ST 422

Query: 427 STCNQSFQGAAFNKL 441
           STCNQ F GA  NKL
Sbjct: 423 STCNQHFLGAILNKL 434

BLAST of CSPI01G25960 vs. ExPASy TrEMBL
Match: A0A1S3BPI1 (calmodulin-binding protein 60 B-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492121 PE=3 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 6.0e-210
Identity = 381/436 (87.39%), Postives = 397/436 (91.06%), Query Frame = 0

Query: 7   KTFFRPDQNYQTTSSHFSFPFGFGVGDNHEVLSPNSVAYVENYFRPMIREEVEAKINARF 66
           KTFF P QNYQTTSSH+SFP GFGVGD+H VLSP SVAYVENYFRP+IR+EVEAKINA F
Sbjct: 3   KTFFCP-QNYQTTSSHYSFPSGFGVGDSHLVLSPKSVAYVENYFRPLIRKEVEAKINAHF 62

Query: 67  NVSTNYQSCSNSSTSREGVLGEDRAVVIVGGGRLRTENQQQLKLRFTNKVSSTVFTVNDI 126
           NVST+ QSCSNSSTSREGVLGEDRAVVIVGGGRLR ENQQQLKLRFTNKV ST++TVND+
Sbjct: 63  NVSTD-QSCSNSSTSREGVLGEDRAVVIVGGGRLRKENQQQLKLRFTNKVPSTIYTVNDV 122

Query: 127 EAENGEELRVELFDAVNDRIIDATHPLSSASIEVVVLDGEFNDGEAITQSDFNRSVVPER 186
           EAENGEELRVELFD VNDRIID THPLSSA IEVVVLD EFND EAITQSDFNRSVVP+R
Sbjct: 123 EAENGEELRVELFDVVNDRIIDVTHPLSSARIEVVVLDREFNDEEAITQSDFNRSVVPQR 182

Query: 187 LGERPLLVGRDKRFRLEKGVYSITDLSFTRNSSRSRTKKICLGLRVTQDS-NNNYPTIGH 246
           LGERPLLVG  KRFRLE GVYSITDLSFTRNSSR RTKKI LGLRV  DS NNNYPTIGH
Sbjct: 183 LGERPLLVGGHKRFRLENGVYSITDLSFTRNSSRCRTKKIRLGLRVINDSNNNNYPTIGH 242

Query: 247 TVSNPFRVKDHRGQLNKKHHPPKGEDEVWRLEGIGRNGEYHKRLTSHTILNVDDFLKAYQ 306
            VSNPFRVKDHRGQLN+K HPPKGEDEVWRLEGIGRNGEYHKRLTSH IL VDDFLKAYQ
Sbjct: 243 AVSNPFRVKDHRGQLNQKRHPPKGEDEVWRLEGIGRNGEYHKRLTSHGILYVDDFLKAYQ 302

Query: 307 KDSRSLRKWLGNRVSEKKWKSMVKHAEEYVPITNAIVPTFDPPTGF-QQNLVENEAMGGV 366
           KDSRSLRKWLGNRVS+KKWKSMVKHA EYVPITN IVPTFDPPT F QQNLVEN+AMGGV
Sbjct: 303 KDSRSLRKWLGNRVSDKKWKSMVKHAHEYVPITNTIVPTFDPPTSFQQQNLVENKAMGGV 362

Query: 367 EEVSNQNNIGDGFQDHTSLSADVIQDHFYRIFQDYPIQTLLSQGEASTSNGLYNYVDQLS 426
           EEVSNQNNIGDGFQ+HTSL ADVIQDHF+RIFQD+P QTLLSQGEASTSNG YNYV+  S
Sbjct: 363 EEVSNQNNIGDGFQEHTSLLADVIQDHFHRIFQDFPTQTLLSQGEASTSNGYYNYVEH-S 422

Query: 427 TSTCNQSFQGAAFNKL 441
           TSTCNQ F GA  NKL
Sbjct: 423 TSTCNQHFLGAILNKL 435

BLAST of CSPI01G25960 vs. ExPASy TrEMBL
Match: A0A1S3BQ49 (calmodulin-binding protein 60 B-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103492121 PE=3 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 4.2e-187
Identity = 343/393 (87.28%), Postives = 356/393 (90.59%), Query Frame = 0

Query: 50  FRPMIREEVEAKINARFNVSTNYQSCSNSSTSREGVLGEDRAVVIVGGGRLRTENQQQLK 109
           F   IR+EVEAKINA FNVST+ QSCSNSSTSREGVLGEDRAVVIVGGGRLR ENQQQLK
Sbjct: 24  FSIWIRKEVEAKINAHFNVSTD-QSCSNSSTSREGVLGEDRAVVIVGGGRLRKENQQQLK 83

Query: 110 LRFTNKVSSTVFTVNDIEAENGEELRVELFDAVNDRIIDATHPLSSASIEVVVLDGEFND 169
           LRFTNKV ST++TVND+EAENGEELRVELFD VNDRIID THPLSSA IEVVVLD EFND
Sbjct: 84  LRFTNKVPSTIYTVNDVEAENGEELRVELFDVVNDRIIDVTHPLSSARIEVVVLDREFND 143

Query: 170 GEAITQSDFNRSVVPERLGERPLLVGRDKRFRLEKGVYSITDLSFTRNSSRSRTKKICLG 229
            EAITQSDFNRSVVP+RLGERPLLVG  KRFRLE GVYSITDLSFTRNSSR RTKKI LG
Sbjct: 144 EEAITQSDFNRSVVPQRLGERPLLVGGHKRFRLENGVYSITDLSFTRNSSRCRTKKIRLG 203

Query: 230 LRVTQDS-NNNYPTIGHTVSNPFRVKDHRGQLNKKHHPPKGEDEVWRLEGIGRNGEYHKR 289
           LRV  DS NNNYPTIGH VSNPFRVKDHRGQLN+K HPPKGEDEVWRLEGIGRNGEYHKR
Sbjct: 204 LRVINDSNNNNYPTIGHAVSNPFRVKDHRGQLNQKRHPPKGEDEVWRLEGIGRNGEYHKR 263

Query: 290 LTSHTILNVDDFLKAYQKDSRSLRKWLGNRVSEKKWKSMVKHAEEYVPITNAIVPTFDPP 349
           LTSH IL VDDFLKAYQKDSRSLRKWLGNRVS+KKWKSMVKHA EYVPITN IVPTFDPP
Sbjct: 264 LTSHGILYVDDFLKAYQKDSRSLRKWLGNRVSDKKWKSMVKHAHEYVPITNTIVPTFDPP 323

Query: 350 TGF-QQNLVENEAMGGVEEVSNQNNIGDGFQDHTSLSADVIQDHFYRIFQDYPIQTLLSQ 409
           T F QQNLVEN+AMGGVEEVSNQNNIGDGFQ+HTSL ADVIQDHF+RIFQD+P QTLLSQ
Sbjct: 324 TSFQQQNLVENKAMGGVEEVSNQNNIGDGFQEHTSLLADVIQDHFHRIFQDFPTQTLLSQ 383

Query: 410 GEASTSNGLYNYVDQLSTSTCNQSFQGAAFNKL 441
           GEASTSNG YNYV+  STSTCNQ F GA  NKL
Sbjct: 384 GEASTSNGYYNYVEH-STSTCNQHFLGAILNKL 414

BLAST of CSPI01G25960 vs. ExPASy TrEMBL
Match: A0A5D3CGD6 (Calmodulin-binding protein 60 B-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004390 PE=3 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 3.9e-169
Identity = 308/353 (87.25%), Postives = 322/353 (91.22%), Query Frame = 0

Query: 1   MDYLNTKTFFRPDQNYQTTSSHFSFPFGFGVGDNHEVLSPNSVAYVENYFRPMIREEVEA 60
           M++ + KTFF P QNYQTTSSH+SFP GFGVGD+H VLSP SVAYVENYFRP+IR+EVEA
Sbjct: 1   MEFPDAKTFFCP-QNYQTTSSHYSFPSGFGVGDSHLVLSPKSVAYVENYFRPLIRKEVEA 60

Query: 61  KINARFNVSTNYQSCSNSSTSREGVLGEDRAVVIVGGGRLRTENQQQLKLRFTNKVSSTV 120
           KINA FNVST+ QSCSNSSTSREGVLGEDRAVVIVGGGRLR ENQQQLKLRFTNKV ST+
Sbjct: 61  KINAHFNVSTD-QSCSNSSTSREGVLGEDRAVVIVGGGRLRKENQQQLKLRFTNKVPSTI 120

Query: 121 FTVNDIEAENGEELRVELFDAVNDRIIDATHPLSSASIEVVVLDGEFNDGEAITQSDFNR 180
           +TVND+EAENGEELRVELFD VNDRIID THPLSSA IEVVVLD EFND EAITQSDFNR
Sbjct: 121 YTVNDVEAENGEELRVELFDVVNDRIIDVTHPLSSARIEVVVLDREFNDEEAITQSDFNR 180

Query: 181 SVVPERLGERPLLVGRDKRFRLEKGVYSITDLSFTRNSSRSRTKKICLGLRVTQDS-NNN 240
           SVVP+RLGERPLLVG  KRFRLE GVYSITDLSFTRNSSR RTKKI LGLRV  DS NNN
Sbjct: 181 SVVPQRLGERPLLVGGHKRFRLENGVYSITDLSFTRNSSRCRTKKIRLGLRVINDSNNNN 240

Query: 241 YPTIGHTVSNPFRVKDHRGQLNKKHHPPKGEDEVWRLEGIGRNGEYHKRLTSHTILNVDD 300
           YPTIGH VSNPFRVKDHRGQLN+K HPPKGEDEVWRLEGIGRNGEYHKRLTSH IL VDD
Sbjct: 241 YPTIGHAVSNPFRVKDHRGQLNQKRHPPKGEDEVWRLEGIGRNGEYHKRLTSHGILYVDD 300

Query: 301 FLKAYQKDSRSLRKWLGNRVSEKKWKSMVKHAEEYVPITNAIVPTFDPPTGFQ 353
           FLKAYQKDSRSLRKWLGNRVS+KKWKSMVKHA EYVPITN IVPTFDPPT FQ
Sbjct: 301 FLKAYQKDSRSLRKWLGNRVSDKKWKSMVKHAHEYVPITNTIVPTFDPPTSFQ 351

BLAST of CSPI01G25960 vs. ExPASy TrEMBL
Match: A0A5A7UBI3 (Calmodulin-binding protein 60 B-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold761G00680 PE=3 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 3.9e-169
Identity = 308/353 (87.25%), Postives = 322/353 (91.22%), Query Frame = 0

Query: 1   MDYLNTKTFFRPDQNYQTTSSHFSFPFGFGVGDNHEVLSPNSVAYVENYFRPMIREEVEA 60
           M++ + KTFF P QNYQTTSSH+SFP GFGVGD+H VLSP SVAYVENYFRP+IR+EVEA
Sbjct: 1   MEFPDAKTFFCP-QNYQTTSSHYSFPSGFGVGDSHLVLSPKSVAYVENYFRPLIRKEVEA 60

Query: 61  KINARFNVSTNYQSCSNSSTSREGVLGEDRAVVIVGGGRLRTENQQQLKLRFTNKVSSTV 120
           KINA FNVST+ QSCSNSSTSREGVLGEDRAVVIVGGGRLR ENQQQLKLRFTNKV ST+
Sbjct: 61  KINAHFNVSTD-QSCSNSSTSREGVLGEDRAVVIVGGGRLRKENQQQLKLRFTNKVPSTI 120

Query: 121 FTVNDIEAENGEELRVELFDAVNDRIIDATHPLSSASIEVVVLDGEFNDGEAITQSDFNR 180
           +TVND+EAENGEELRVELFD VNDRIID THPLSSA IEVVVLD EFND EAITQSDFNR
Sbjct: 121 YTVNDVEAENGEELRVELFDVVNDRIIDVTHPLSSARIEVVVLDREFNDEEAITQSDFNR 180

Query: 181 SVVPERLGERPLLVGRDKRFRLEKGVYSITDLSFTRNSSRSRTKKICLGLRVTQDS-NNN 240
           SVVP+RLGERPLLVG  KRFRLE GVYSITDLSFTRNSSR RTKKI LGLRV  DS NNN
Sbjct: 181 SVVPQRLGERPLLVGGHKRFRLENGVYSITDLSFTRNSSRCRTKKIRLGLRVINDSNNNN 240

Query: 241 YPTIGHTVSNPFRVKDHRGQLNKKHHPPKGEDEVWRLEGIGRNGEYHKRLTSHTILNVDD 300
           YPTIGH VSNPFRVKDHRGQLN+K HPPKGEDEVWRLEGIGRNGEYHKRLTSH IL VDD
Sbjct: 241 YPTIGHAVSNPFRVKDHRGQLNQKRHPPKGEDEVWRLEGIGRNGEYHKRLTSHGILYVDD 300

Query: 301 FLKAYQKDSRSLRKWLGNRVSEKKWKSMVKHAEEYVPITNAIVPTFDPPTGFQ 353
           FLKAYQKDSRSLRKWLGNRVS+KKWKSMVKHA EYVPITN IVPTFDPPT FQ
Sbjct: 301 FLKAYQKDSRSLRKWLGNRVSDKKWKSMVKHAHEYVPITNTIVPTFDPPTSFQ 351

BLAST of CSPI01G25960 vs. NCBI nr
Match: XP_004135768.2 (calmodulin-binding protein 60 B isoform X2 [Cucumis sativus] >KAE8653349.1 hypothetical protein Csa_007610 [Cucumis sativus])

HSP 1 Score: 886.7 bits (2290), Expect = 8.2e-254
Identity = 442/445 (99.33%), Postives = 442/445 (99.33%), Query Frame = 0

Query: 1   MDYLNTKTFFRPDQNYQTTSSHFSFPFGFGVGDNHEVLSPNSVAYVENYFRPMIREEVEA 60
           MDYLNTKTFFRPDQNYQTTSSHFSFPFGFGVGDNHEVLSPNSVAYVENYFRPMIREEVEA
Sbjct: 1   MDYLNTKTFFRPDQNYQTTSSHFSFPFGFGVGDNHEVLSPNSVAYVENYFRPMIREEVEA 60

Query: 61  KINARFNVSTNYQSCSNSSTSREGVLGEDRAVVIVGGGRLRTENQQQLKLRFTNKVSSTV 120
           KINARFNVSTNYQSCSNSSTSREGVLGEDRAVVIVGGGRLRTENQQQLKLRFTNKVSSTV
Sbjct: 61  KINARFNVSTNYQSCSNSSTSREGVLGEDRAVVIVGGGRLRTENQQQLKLRFTNKVSSTV 120

Query: 121 FTVNDIEAENGEELRVELFDAVNDRIIDATHPLSSASIEVVVLDGEFNDGEAITQSDFNR 180
           FTVNDIEAENGEELRVELFDAVNDRIIDATHPLSSASIEVVVLDGEFNDGEAITQSDFNR
Sbjct: 121 FTVNDIEAENGEELRVELFDAVNDRIIDATHPLSSASIEVVVLDGEFNDGEAITQSDFNR 180

Query: 181 SVVPERLGERPLLVGRDKRFRLEKGVYSITDLSFTRNSSRSRTKKICLGLRVTQDSNNNY 240
           SVVPERLGERPLLVGRDKRFRLEKGVYSITDLSFTRNSSRSRTKKICLGLRVTQDSNNNY
Sbjct: 181 SVVPERLGERPLLVGRDKRFRLEKGVYSITDLSFTRNSSRSRTKKICLGLRVTQDSNNNY 240

Query: 241 PTIGHTVSNPFRVKDHRGQLNKKHHPPKGEDEVWRLEGIGRNGEYHKRLTSHTILNVDDF 300
           PTIGHTVSNPFRVKDHRGQLNKKHHPPKGEDEVWRLEGIGRNGEYHKRLTSHTILNVDDF
Sbjct: 241 PTIGHTVSNPFRVKDHRGQLNKKHHPPKGEDEVWRLEGIGRNGEYHKRLTSHTILNVDDF 300

Query: 301 LKAYQKDSRSLRKWLGNRVSEKKWKSMVKHAEEYVPITNAIVPTFDPPTGFQQNLVENEA 360
           LKAYQKDSRSLRKWLGNRVSEKKWKSMVKHAEEYVPITNA VPTFDP T FQQNLVENEA
Sbjct: 301 LKAYQKDSRSLRKWLGNRVSEKKWKSMVKHAEEYVPITNAFVPTFDPLTSFQQNLVENEA 360

Query: 361 MGGVEEVSNQNNIGDGFQDHTSLSADVIQDHFYRIFQDYPIQTLLSQGEASTSNGLYNYV 420
           MGGVEEVSNQNNIGDGFQDHTSLSADVIQDHFYRIFQDYPIQTLLSQGEASTSNGLYNYV
Sbjct: 361 MGGVEEVSNQNNIGDGFQDHTSLSADVIQDHFYRIFQDYPIQTLLSQGEASTSNGLYNYV 420

Query: 421 DQLSTSTCNQSFQGAAFNKLVPKSK 446
           DQLSTSTCNQSFQGAAFNKLVPKSK
Sbjct: 421 DQLSTSTCNQSFQGAAFNKLVPKSK 445

BLAST of CSPI01G25960 vs. NCBI nr
Match: XP_011659615.1 (calmodulin-binding protein 60 B isoform X1 [Cucumis sativus])

HSP 1 Score: 882.1 bits (2278), Expect = 2.0e-252
Identity = 442/446 (99.10%), Postives = 442/446 (99.10%), Query Frame = 0

Query: 1   MDYLNTKTFFRPDQNYQTTSSHFSFPFGFGVGDNHEVLSPNSVAYVENYFRPMIREEVEA 60
           MDYLNTKTFFRPDQNYQTTSSHFSFPFGFGVGDNHEVLSPNSVAYVENYFRPMIREEVEA
Sbjct: 1   MDYLNTKTFFRPDQNYQTTSSHFSFPFGFGVGDNHEVLSPNSVAYVENYFRPMIREEVEA 60

Query: 61  KINARFNVSTNYQSCSNSSTSREGVLGEDRAVVIVGGGRLRTENQQQLKLRFTNKVSSTV 120
           KINARFNVSTNYQSCSNSSTSREGVLGEDRAVVIVGGGRLRTENQQQLKLRFTNKVSSTV
Sbjct: 61  KINARFNVSTNYQSCSNSSTSREGVLGEDRAVVIVGGGRLRTENQQQLKLRFTNKVSSTV 120

Query: 121 FTVNDIEAENGEELRVELFDAVNDRIIDATHPLSSASIEVVVLDGEFNDGEAITQSDFNR 180
           FTVNDIEAENGEELRVELFDAVNDRIIDATHPLSSASIEVVVLDGEFNDGEAITQSDFNR
Sbjct: 121 FTVNDIEAENGEELRVELFDAVNDRIIDATHPLSSASIEVVVLDGEFNDGEAITQSDFNR 180

Query: 181 SVVPERLGERPLLVGRDKRFRLEKGVYSITDLSFTRNSSRSRTKKICLGLRVTQDSNNNY 240
           SVVPERLGERPLLVGRDKRFRLEKGVYSITDLSFTRNSSRSRTKKICLGLRVTQDSNNNY
Sbjct: 181 SVVPERLGERPLLVGRDKRFRLEKGVYSITDLSFTRNSSRSRTKKICLGLRVTQDSNNNY 240

Query: 241 PTIGHTVSNPFRVKDHRGQLNKKHHPPKGEDEVWRLEGIGRNGEYHKRLTSHTILNVDDF 300
           PTIGHTVSNPFRVKDHRGQLNKKHHPPKGEDEVWRLEGIGRNGEYHKRLTSHTILNVDDF
Sbjct: 241 PTIGHTVSNPFRVKDHRGQLNKKHHPPKGEDEVWRLEGIGRNGEYHKRLTSHTILNVDDF 300

Query: 301 LKAYQKDSRSLRKWLGNRVSEKKWKSMVKHAEEYVPITNAIVPTFDPPTGF-QQNLVENE 360
           LKAYQKDSRSLRKWLGNRVSEKKWKSMVKHAEEYVPITNA VPTFDP T F QQNLVENE
Sbjct: 301 LKAYQKDSRSLRKWLGNRVSEKKWKSMVKHAEEYVPITNAFVPTFDPLTSFQQQNLVENE 360

Query: 361 AMGGVEEVSNQNNIGDGFQDHTSLSADVIQDHFYRIFQDYPIQTLLSQGEASTSNGLYNY 420
           AMGGVEEVSNQNNIGDGFQDHTSLSADVIQDHFYRIFQDYPIQTLLSQGEASTSNGLYNY
Sbjct: 361 AMGGVEEVSNQNNIGDGFQDHTSLSADVIQDHFYRIFQDYPIQTLLSQGEASTSNGLYNY 420

Query: 421 VDQLSTSTCNQSFQGAAFNKLVPKSK 446
           VDQLSTSTCNQSFQGAAFNKLVPKSK
Sbjct: 421 VDQLSTSTCNQSFQGAAFNKLVPKSK 446

BLAST of CSPI01G25960 vs. NCBI nr
Match: XP_008450558.1 (PREDICTED: calmodulin-binding protein 60 B-like isoform X2 [Cucumis melo])

HSP 1 Score: 744.6 bits (1921), Expect = 5.0e-211
Identity = 381/435 (87.59%), Postives = 397/435 (91.26%), Query Frame = 0

Query: 7   KTFFRPDQNYQTTSSHFSFPFGFGVGDNHEVLSPNSVAYVENYFRPMIREEVEAKINARF 66
           KTFF P QNYQTTSSH+SFP GFGVGD+H VLSP SVAYVENYFRP+IR+EVEAKINA F
Sbjct: 3   KTFFCP-QNYQTTSSHYSFPSGFGVGDSHLVLSPKSVAYVENYFRPLIRKEVEAKINAHF 62

Query: 67  NVSTNYQSCSNSSTSREGVLGEDRAVVIVGGGRLRTENQQQLKLRFTNKVSSTVFTVNDI 126
           NVST+ QSCSNSSTSREGVLGEDRAVVIVGGGRLR ENQQQLKLRFTNKV ST++TVND+
Sbjct: 63  NVSTD-QSCSNSSTSREGVLGEDRAVVIVGGGRLRKENQQQLKLRFTNKVPSTIYTVNDV 122

Query: 127 EAENGEELRVELFDAVNDRIIDATHPLSSASIEVVVLDGEFNDGEAITQSDFNRSVVPER 186
           EAENGEELRVELFD VNDRIID THPLSSA IEVVVLD EFND EAITQSDFNRSVVP+R
Sbjct: 123 EAENGEELRVELFDVVNDRIIDVTHPLSSARIEVVVLDREFNDEEAITQSDFNRSVVPQR 182

Query: 187 LGERPLLVGRDKRFRLEKGVYSITDLSFTRNSSRSRTKKICLGLRVTQDS-NNNYPTIGH 246
           LGERPLLVG  KRFRLE GVYSITDLSFTRNSSR RTKKI LGLRV  DS NNNYPTIGH
Sbjct: 183 LGERPLLVGGHKRFRLENGVYSITDLSFTRNSSRCRTKKIRLGLRVINDSNNNNYPTIGH 242

Query: 247 TVSNPFRVKDHRGQLNKKHHPPKGEDEVWRLEGIGRNGEYHKRLTSHTILNVDDFLKAYQ 306
            VSNPFRVKDHRGQLN+K HPPKGEDEVWRLEGIGRNGEYHKRLTSH IL VDDFLKAYQ
Sbjct: 243 AVSNPFRVKDHRGQLNQKRHPPKGEDEVWRLEGIGRNGEYHKRLTSHGILYVDDFLKAYQ 302

Query: 307 KDSRSLRKWLGNRVSEKKWKSMVKHAEEYVPITNAIVPTFDPPTGFQQNLVENEAMGGVE 366
           KDSRSLRKWLGNRVS+KKWKSMVKHA EYVPITN IVPTFDPPT FQQNLVEN+AMGGVE
Sbjct: 303 KDSRSLRKWLGNRVSDKKWKSMVKHAHEYVPITNTIVPTFDPPTSFQQNLVENKAMGGVE 362

Query: 367 EVSNQNNIGDGFQDHTSLSADVIQDHFYRIFQDYPIQTLLSQGEASTSNGLYNYVDQLST 426
           EVSNQNNIGDGFQ+HTSL ADVIQDHF+RIFQD+P QTLLSQGEASTSNG YNYV+  ST
Sbjct: 363 EVSNQNNIGDGFQEHTSLLADVIQDHFHRIFQDFPTQTLLSQGEASTSNGYYNYVEH-ST 422

Query: 427 STCNQSFQGAAFNKL 441
           STCNQ F GA  NKL
Sbjct: 423 STCNQHFLGAILNKL 434

BLAST of CSPI01G25960 vs. NCBI nr
Match: XP_008450557.1 (PREDICTED: calmodulin-binding protein 60 B-like isoform X1 [Cucumis melo])

HSP 1 Score: 740.0 bits (1909), Expect = 1.2e-209
Identity = 381/436 (87.39%), Postives = 397/436 (91.06%), Query Frame = 0

Query: 7   KTFFRPDQNYQTTSSHFSFPFGFGVGDNHEVLSPNSVAYVENYFRPMIREEVEAKINARF 66
           KTFF P QNYQTTSSH+SFP GFGVGD+H VLSP SVAYVENYFRP+IR+EVEAKINA F
Sbjct: 3   KTFFCP-QNYQTTSSHYSFPSGFGVGDSHLVLSPKSVAYVENYFRPLIRKEVEAKINAHF 62

Query: 67  NVSTNYQSCSNSSTSREGVLGEDRAVVIVGGGRLRTENQQQLKLRFTNKVSSTVFTVNDI 126
           NVST+ QSCSNSSTSREGVLGEDRAVVIVGGGRLR ENQQQLKLRFTNKV ST++TVND+
Sbjct: 63  NVSTD-QSCSNSSTSREGVLGEDRAVVIVGGGRLRKENQQQLKLRFTNKVPSTIYTVNDV 122

Query: 127 EAENGEELRVELFDAVNDRIIDATHPLSSASIEVVVLDGEFNDGEAITQSDFNRSVVPER 186
           EAENGEELRVELFD VNDRIID THPLSSA IEVVVLD EFND EAITQSDFNRSVVP+R
Sbjct: 123 EAENGEELRVELFDVVNDRIIDVTHPLSSARIEVVVLDREFNDEEAITQSDFNRSVVPQR 182

Query: 187 LGERPLLVGRDKRFRLEKGVYSITDLSFTRNSSRSRTKKICLGLRVTQDS-NNNYPTIGH 246
           LGERPLLVG  KRFRLE GVYSITDLSFTRNSSR RTKKI LGLRV  DS NNNYPTIGH
Sbjct: 183 LGERPLLVGGHKRFRLENGVYSITDLSFTRNSSRCRTKKIRLGLRVINDSNNNNYPTIGH 242

Query: 247 TVSNPFRVKDHRGQLNKKHHPPKGEDEVWRLEGIGRNGEYHKRLTSHTILNVDDFLKAYQ 306
            VSNPFRVKDHRGQLN+K HPPKGEDEVWRLEGIGRNGEYHKRLTSH IL VDDFLKAYQ
Sbjct: 243 AVSNPFRVKDHRGQLNQKRHPPKGEDEVWRLEGIGRNGEYHKRLTSHGILYVDDFLKAYQ 302

Query: 307 KDSRSLRKWLGNRVSEKKWKSMVKHAEEYVPITNAIVPTFDPPTGF-QQNLVENEAMGGV 366
           KDSRSLRKWLGNRVS+KKWKSMVKHA EYVPITN IVPTFDPPT F QQNLVEN+AMGGV
Sbjct: 303 KDSRSLRKWLGNRVSDKKWKSMVKHAHEYVPITNTIVPTFDPPTSFQQQNLVENKAMGGV 362

Query: 367 EEVSNQNNIGDGFQDHTSLSADVIQDHFYRIFQDYPIQTLLSQGEASTSNGLYNYVDQLS 426
           EEVSNQNNIGDGFQ+HTSL ADVIQDHF+RIFQD+P QTLLSQGEASTSNG YNYV+  S
Sbjct: 363 EEVSNQNNIGDGFQEHTSLLADVIQDHFHRIFQDFPTQTLLSQGEASTSNGYYNYVEH-S 422

Query: 427 TSTCNQSFQGAAFNKL 441
           TSTCNQ F GA  NKL
Sbjct: 423 TSTCNQHFLGAILNKL 435

BLAST of CSPI01G25960 vs. NCBI nr
Match: XP_008450559.1 (PREDICTED: calmodulin-binding protein 60 B-like isoform X3 [Cucumis melo])

HSP 1 Score: 664.1 bits (1712), Expect = 8.6e-187
Identity = 343/393 (87.28%), Postives = 356/393 (90.59%), Query Frame = 0

Query: 50  FRPMIREEVEAKINARFNVSTNYQSCSNSSTSREGVLGEDRAVVIVGGGRLRTENQQQLK 109
           F   IR+EVEAKINA FNVST+ QSCSNSSTSREGVLGEDRAVVIVGGGRLR ENQQQLK
Sbjct: 24  FSIWIRKEVEAKINAHFNVSTD-QSCSNSSTSREGVLGEDRAVVIVGGGRLRKENQQQLK 83

Query: 110 LRFTNKVSSTVFTVNDIEAENGEELRVELFDAVNDRIIDATHPLSSASIEVVVLDGEFND 169
           LRFTNKV ST++TVND+EAENGEELRVELFD VNDRIID THPLSSA IEVVVLD EFND
Sbjct: 84  LRFTNKVPSTIYTVNDVEAENGEELRVELFDVVNDRIIDVTHPLSSARIEVVVLDREFND 143

Query: 170 GEAITQSDFNRSVVPERLGERPLLVGRDKRFRLEKGVYSITDLSFTRNSSRSRTKKICLG 229
            EAITQSDFNRSVVP+RLGERPLLVG  KRFRLE GVYSITDLSFTRNSSR RTKKI LG
Sbjct: 144 EEAITQSDFNRSVVPQRLGERPLLVGGHKRFRLENGVYSITDLSFTRNSSRCRTKKIRLG 203

Query: 230 LRVTQDS-NNNYPTIGHTVSNPFRVKDHRGQLNKKHHPPKGEDEVWRLEGIGRNGEYHKR 289
           LRV  DS NNNYPTIGH VSNPFRVKDHRGQLN+K HPPKGEDEVWRLEGIGRNGEYHKR
Sbjct: 204 LRVINDSNNNNYPTIGHAVSNPFRVKDHRGQLNQKRHPPKGEDEVWRLEGIGRNGEYHKR 263

Query: 290 LTSHTILNVDDFLKAYQKDSRSLRKWLGNRVSEKKWKSMVKHAEEYVPITNAIVPTFDPP 349
           LTSH IL VDDFLKAYQKDSRSLRKWLGNRVS+KKWKSMVKHA EYVPITN IVPTFDPP
Sbjct: 264 LTSHGILYVDDFLKAYQKDSRSLRKWLGNRVSDKKWKSMVKHAHEYVPITNTIVPTFDPP 323

Query: 350 TGF-QQNLVENEAMGGVEEVSNQNNIGDGFQDHTSLSADVIQDHFYRIFQDYPIQTLLSQ 409
           T F QQNLVEN+AMGGVEEVSNQNNIGDGFQ+HTSL ADVIQDHF+RIFQD+P QTLLSQ
Sbjct: 324 TSFQQQNLVENKAMGGVEEVSNQNNIGDGFQEHTSLLADVIQDHFHRIFQDFPTQTLLSQ 383

Query: 410 GEASTSNGLYNYVDQLSTSTCNQSFQGAAFNKL 441
           GEASTSNG YNYV+  STSTCNQ F GA  NKL
Sbjct: 384 GEASTSNGYYNYVEH-STSTCNQHFLGAILNKL 414

BLAST of CSPI01G25960 vs. TAIR 10
Match: AT5G57580.1 (Calmodulin-binding protein )

HSP 1 Score: 169.1 bits (427), Expect = 8.1e-42
Identity = 100/239 (41.84%), Postives = 146/239 (61.09%), Query Frame = 0

Query: 99  RLRTENQQQLKLRFTNKVSSTVFTVNDIEAENGEELRVELFDAVNDRIIDATHPLSSASI 158
           R+   + ++L+L F +++S  +FT   +E E G  + V L DA   R +    P +SA +
Sbjct: 85  RIEGPDGRKLQLHFKSRLSLPLFTGGKVEGEQGAVIHVVLIDANTGRAV-VYGPEASAKL 144

Query: 159 EVVVLDGEFN--DGEAITQSDFNRSVVPERLGERPLLVGRDKRFRLEKGVYSITDLSFTR 218
            +VVL+G+FN  D E  TQ +F   VV ER G+RPLL G +    L++GV ++ +L FT 
Sbjct: 145 HIVVLEGDFNTEDDEDWTQEEFESHVVKERSGKRPLLTG-EVYVTLKEGVGTLGELVFTD 204

Query: 219 NSSRSRTKKICLGLRVTQDSNNNYPTIGHTVSNPFRVKDHRGQLNKKHHPPKGEDEVWRL 278
           NSS  R++K  LGLRV     +    I    +  F VKDHRG+L KKH+PP   D+VWRL
Sbjct: 205 NSSWIRSRKFRLGLRVVSGCCDGM-RIREAKTEAFVVKDHRGELYKKHYPPALNDDVWRL 264

Query: 279 EGIGRNGEYHKRLTSHTILNVDDFLKAYQKDSRSLRKWLGNRVSEKKWKSMVKHAEEYV 336
           + IG++G +HK+LT+  I  V+DFL+   KDS  LR  LG+ +S K W ++V+HA+  V
Sbjct: 265 DKIGKDGAFHKKLTAEGINTVEDFLRVMVKDSPKLRTILGSGMSNKMWDALVEHAKTCV 320

BLAST of CSPI01G25960 vs. TAIR 10
Match: AT2G18750.1 (Calmodulin-binding protein )

HSP 1 Score: 165.6 bits (418), Expect = 9.0e-41
Identity = 103/274 (37.59%), Postives = 157/274 (57.30%), Query Frame = 0

Query: 75  CSNSSTSREGVLGE--DRAVVIVGGGRLRTENQ---------QQLKLRFTNKVSSTVFTV 134
           CS+       V+ E  +RA+  +G  RL   +          + L+L+F +++S  +FT 
Sbjct: 54  CSSLEPILRRVVSEEVERALAKLGPARLSERSSPKRIEGIGGRNLQLQFRSRLSVPLFTG 113

Query: 135 NDIEAENGEELRVELFDAVNDRIIDATHPLSSASIEVVVLDGEFN--DGEAITQSDFNRS 194
             IE E G  + V L D     ++    P +SA ++VVVLDG+FN  D +  +  +F   
Sbjct: 114 GKIEGEQGAAIHVVLLDMTTGHVL-TVGPEASAKLDVVVLDGDFNTEDDDGWSGEEFEGH 173

Query: 195 VVPERLGERPLLVGRDKRFRLEKGVYSITDLSFTRNSSRSRTKKICLGLRVTQDSNNNYP 254
           +V ER G+RPLL G D +  L++GV ++ +L FT NSS  R +K  LGLRV+        
Sbjct: 174 LVKERQGKRPLLTG-DVQVTLKEGVGTLGELIFTDNSSWIRCRKFRLGLRVSSGYCEGM- 233

Query: 255 TIGHTVSNPFRVKDHRGQLNKKHHPPKGEDEVWRLEGIGRNGEYHKRLTSHTILNVDDFL 314
            +    +  F VKDHRG+L KKH+PP  +DEVWRLE IG++G +HK+L    I NV +FL
Sbjct: 234 RVREAKTEAFTVKDHRGELYKKHYPPALDDEVWRLEKIGKDGAFHKKLNKAGIYNVKEFL 293

Query: 315 KAYQKDSRSLRKWLGNRVSEKKWKSMVKHAEEYV 336
           +   KDS+ LR  LG+ +S + W+++ +H++  V
Sbjct: 294 RLMVKDSQKLRTILGSGMSNRMWETLAEHSKTCV 324

BLAST of CSPI01G25960 vs. TAIR 10
Match: AT2G18750.2 (Calmodulin-binding protein )

HSP 1 Score: 165.6 bits (418), Expect = 9.0e-41
Identity = 103/274 (37.59%), Postives = 157/274 (57.30%), Query Frame = 0

Query: 75  CSNSSTSREGVLGE--DRAVVIVGGGRLRTENQ---------QQLKLRFTNKVSSTVFTV 134
           CS+       V+ E  +RA+  +G  RL   +          + L+L+F +++S  +FT 
Sbjct: 54  CSSLEPILRRVVSEEVERALAKLGPARLSERSSPKRIEGIGGRNLQLQFRSRLSVPLFTG 113

Query: 135 NDIEAENGEELRVELFDAVNDRIIDATHPLSSASIEVVVLDGEFN--DGEAITQSDFNRS 194
             IE E G  + V L D     ++    P +SA ++VVVLDG+FN  D +  +  +F   
Sbjct: 114 GKIEGEQGAAIHVVLLDMTTGHVL-TVGPEASAKLDVVVLDGDFNTEDDDGWSGEEFEGH 173

Query: 195 VVPERLGERPLLVGRDKRFRLEKGVYSITDLSFTRNSSRSRTKKICLGLRVTQDSNNNYP 254
           +V ER G+RPLL G D +  L++GV ++ +L FT NSS  R +K  LGLRV+        
Sbjct: 174 LVKERQGKRPLLTG-DVQVTLKEGVGTLGELIFTDNSSWIRCRKFRLGLRVSSGYCEGM- 233

Query: 255 TIGHTVSNPFRVKDHRGQLNKKHHPPKGEDEVWRLEGIGRNGEYHKRLTSHTILNVDDFL 314
            +    +  F VKDHRG+L KKH+PP  +DEVWRLE IG++G +HK+L    I NV +FL
Sbjct: 234 RVREAKTEAFTVKDHRGELYKKHYPPALDDEVWRLEKIGKDGAFHKKLNKAGIYNVKEFL 293

Query: 315 KAYQKDSRSLRKWLGNRVSEKKWKSMVKHAEEYV 336
           +   KDS+ LR  LG+ +S + W+++ +H++  V
Sbjct: 294 RLMVKDSQKLRTILGSGMSNRMWETLAEHSKTCV 324

BLAST of CSPI01G25960 vs. TAIR 10
Match: AT2G18750.3 (Calmodulin-binding protein )

HSP 1 Score: 165.6 bits (418), Expect = 9.0e-41
Identity = 103/274 (37.59%), Postives = 157/274 (57.30%), Query Frame = 0

Query: 75  CSNSSTSREGVLGE--DRAVVIVGGGRLRTENQ---------QQLKLRFTNKVSSTVFTV 134
           CS+       V+ E  +RA+  +G  RL   +          + L+L+F +++S  +FT 
Sbjct: 54  CSSLEPILRRVVSEEVERALAKLGPARLSERSSPKRIEGIGGRNLQLQFRSRLSVPLFTG 113

Query: 135 NDIEAENGEELRVELFDAVNDRIIDATHPLSSASIEVVVLDGEFN--DGEAITQSDFNRS 194
             IE E G  + V L D     ++    P +SA ++VVVLDG+FN  D +  +  +F   
Sbjct: 114 GKIEGEQGAAIHVVLLDMTTGHVL-TVGPEASAKLDVVVLDGDFNTEDDDGWSGEEFEGH 173

Query: 195 VVPERLGERPLLVGRDKRFRLEKGVYSITDLSFTRNSSRSRTKKICLGLRVTQDSNNNYP 254
           +V ER G+RPLL G D +  L++GV ++ +L FT NSS  R +K  LGLRV+        
Sbjct: 174 LVKERQGKRPLLTG-DVQVTLKEGVGTLGELIFTDNSSWIRCRKFRLGLRVSSGYCEGM- 233

Query: 255 TIGHTVSNPFRVKDHRGQLNKKHHPPKGEDEVWRLEGIGRNGEYHKRLTSHTILNVDDFL 314
            +    +  F VKDHRG+L KKH+PP  +DEVWRLE IG++G +HK+L    I NV +FL
Sbjct: 234 RVREAKTEAFTVKDHRGELYKKHYPPALDDEVWRLEKIGKDGAFHKKLNKAGIYNVKEFL 293

Query: 315 KAYQKDSRSLRKWLGNRVSEKKWKSMVKHAEEYV 336
           +   KDS+ LR  LG+ +S + W+++ +H++  V
Sbjct: 294 RLMVKDSQKLRTILGSGMSNRMWETLAEHSKTCV 324

BLAST of CSPI01G25960 vs. TAIR 10
Match: AT4G25800.1 (Calmodulin-binding protein )

HSP 1 Score: 165.6 bits (418), Expect = 9.0e-41
Identity = 102/239 (42.68%), Postives = 143/239 (59.83%), Query Frame = 0

Query: 99  RLRTENQQQLKLRFTNKVSSTVFTVNDIEAENGEELRVELFDAVNDRIIDATHPLSSASI 158
           R+   + + L+L F +++S  +FT   +E E G  + V L DA   R +    P +S  +
Sbjct: 74  RIGGPDGRNLQLHFKSRLSLPLFTGGRVEGEQGATIHVVLIDANTGRPV-TVGPEASLKL 133

Query: 159 EVVVLDGEFN--DGEAITQSDFNRSVVPERLGERPLLVGRDKRFRLEKGVYSITDLSFTR 218
           EVVVL G+FN  D E  TQ +F   VV ER G+RPLL G D    L++GV ++ ++ FT 
Sbjct: 134 EVVVLGGDFNNEDDEDWTQEEFESHVVKEREGKRPLLTG-DLFVVLKEGVGTLGEIVFTD 193

Query: 219 NSSRSRTKKICLGLRVTQDSNNNYPTIGHTVSNPFRVKDHRGQLNKKHHPPKGEDEVWRL 278
           NSS  R++K  LGLRV     +    I    +  F VKDHRG+L KKH+PP   DEVWRL
Sbjct: 194 NSSWIRSRKFRLGLRVPSGYCDGI-RIREAKTEAFSVKDHRGELYKKHYPPALNDEVWRL 253

Query: 279 EGIGRNGEYHKRLTSHTILNVDDFLKAYQKDSRSLRKWLGNRVSEKKWKSMVKHAEEYV 336
           E IG++G +HKRLT+  I+ V+ FL+   +DS  LR  LG+ +S K W  +V+HA+  V
Sbjct: 254 EKIGKDGAFHKRLTAAGIVTVEGFLRQLVRDSTKLRAILGSGMSNKMWDLLVEHAKTCV 309

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FKL61.1e-4041.84Calmodulin-binding protein 60 B OS=Arabidopsis thaliana OX=3702 GN=CBP60B PE=2 S... [more]
C0SV511.3e-3937.59Calmodulin-binding protein 60 C OS=Arabidopsis thaliana OX=3702 GN=CBP60C PE=2 S... [more]
Q0WVV61.3e-3942.68Calmodulin-binding protein 60 D OS=Arabidopsis thaliana OX=3702 GN=CBP60D PE=2 S... [more]
C0SVV63.7e-3939.52Calmodulin-binding protein 60 A OS=Arabidopsis thaliana OX=3702 GN=CBP60A PE=2 S... [more]
F4IPM31.8e-3834.59Calmodulin-binding protein 60 E OS=Arabidopsis thaliana OX=3702 GN=CBP60E PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A1S3BPF42.4e-21187.59calmodulin-binding protein 60 B-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A1S3BPI16.0e-21087.39calmodulin-binding protein 60 B-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A1S3BQ494.2e-18787.28calmodulin-binding protein 60 B-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A5D3CGD63.9e-16987.25Calmodulin-binding protein 60 B-like isoform X2 OS=Cucumis melo var. makuwa OX=1... [more]
A0A5A7UBI33.9e-16987.25Calmodulin-binding protein 60 B-like isoform X1 OS=Cucumis melo var. makuwa OX=1... [more]
Match NameE-valueIdentityDescription
XP_004135768.28.2e-25499.33calmodulin-binding protein 60 B isoform X2 [Cucumis sativus] >KAE8653349.1 hypot... [more]
XP_011659615.12.0e-25299.10calmodulin-binding protein 60 B isoform X1 [Cucumis sativus][more]
XP_008450558.15.0e-21187.59PREDICTED: calmodulin-binding protein 60 B-like isoform X2 [Cucumis melo][more]
XP_008450557.11.2e-20987.39PREDICTED: calmodulin-binding protein 60 B-like isoform X1 [Cucumis melo][more]
XP_008450559.18.6e-18787.28PREDICTED: calmodulin-binding protein 60 B-like isoform X3 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT5G57580.18.1e-4241.84Calmodulin-binding protein [more]
AT2G18750.19.0e-4137.59Calmodulin-binding protein [more]
AT2G18750.29.0e-4137.59Calmodulin-binding protein [more]
AT2G18750.39.0e-4137.59Calmodulin-binding protein [more]
AT4G25800.19.0e-4142.68Calmodulin-binding protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012416CALMODULIN-BINDING PROTEIN60PFAMPF07887Calmodulin_bindcoord: 108..336
e-value: 3.0E-73
score: 246.7
IPR012416CALMODULIN-BINDING PROTEIN60PANTHERPTHR31713OS02G0177800 PROTEINcoord: 41..337
NoneNo IPR availablePANTHERPTHR31713:SF43CALMODULIN-BINDING PROTEIN 60 Gcoord: 41..337

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G25960.1CSPI01G25960.1mRNA
CSPI01G25960.2CSPI01G25960.2mRNA
CSPI01G25960.3CSPI01G25960.3mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005516 calmodulin binding