CSPI01G24800 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G24800
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	FAD-binding Berberine family protein
Location	Chr1: 20223778 .. 20225685 (+)
RNA-Seq Expression	CSPI01G24800
Synteny	CSPI01G24800

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAAAGGAAATTGGCAAGAAACAATGTTGTATTCAGGCTCTTCCATTTTGATTCTCTTACTTCCCTTTCTTCTCTCATTTTCAGTGCTTTCAGTCTCAGCTTCATTGGAGCAAAGTTTTCTACAATGTGTATCTCTCAATTCTCAACAATCTGTTCCTCCATTGTCTGTTTGTTCTCAGAACAATCCTTCATTCTTCCCTCTCCTTCAATCAACTGCACAAAACCTCAGGTTCTTGGACCCTTCAGTTCCCAAGCCTCAGTTTATCTTCACTCCATTGCATGACACTCATGTCCAAGCAGCGGTTATTTGTTCGAAGAAGCTCAACATACATCTTCGAGTTCGTAGTGGTGGCCATGACTATGAGGGTCTTTCCTACGCTTCCGAAACCGAAACACCTTTCATTGTCATAGACCTAGCGAAACTCAGGTCGGTGGAAGTGAATATCCAAGATAACACTGCATGGGCTCAGGCTGGTGCAACGGTTGGTGAAGTTTACTATAGAATCTCACAGAAAAGCAATGTCCATGGCTTCCCTGCTGGCCTTTGCACTAGCTTAGGCATTGGTGGCCACATAACAGGCGGTGCATATGGGTCCATGATGAGAAAATATGGCCTTGGTGCTGATAATGTCATTGATGCTCGGATTGTCGATGTTAATGGTAGAATCCTCGATAGATCCACGATGGGAGAGGACTACTTTTGGGCCATTAGAGGGGGTGCTGGAGGTAGCTTTGGGATTATTCTTTGGTGGAAGTTGCAATTGGTTCCTGTTCCAGAAACTGTGACAGTTTTCACTGTTCCAAAGACATTAGAGCAAGGTGCCACTCAAATCCTGTACAAATGGCAACAAGTTGCTGACAAGCTGGATGAAGATTTGTTCATTAGAGTCATAATACAAGTAACAAATGATAAAGCTACGAAAGAAAGAACCATTACCACAGCTTACAATGCTCTCTTTCTTGGAGATTCCGACAGACTCCTACAAATTATGAGAAAAAGCTTCCCTGAATTGGGTTTGACACCAAAAGATTGTATTGAAACCAACTGGATTAAATCAGTTCTTTACATAGCTGGAGAACCACCAAAAACACCACCTGAAGTTCTTCTACAAGGAAAGCCACAATTCAAGAACTACTTCAAAGCTAAATCAGACTTTGTTCAAGTCCCAATCCCAGAAACTGGATTGGAAGGTCTATGGAAAATATTTCTCCAAGAAGAAAGCCCATTGATGATCTGGAATCCTTATGGAGGAATGATGAGCAAGATCTCAGAGAATGAAATCCCATTCCCCCACAGAAAAGGAAACTTGTTCAAAATTCAGTATGTAAACCCATGGCAGGATGGAGATAAACACGAAACAGAACACATAGAGTGGATCAGAGAACTTTACAACTACATGGCTCCATATGTTTCCAAGTCTCCAAGAGCTGCATATGTAAACTACAGAGATCTTGATTTGGGTATGAACAAGGACAATACAAGCTTGGCTCATGCCACTGAATGGGGCAACAGATATTTCAAGAACAACTTCAATAGGCTTGTGAAGGTAAAGACAAAAGTTGATCCTGGCAACTTTTTCAGGCATGAACAGAGCATTCCTCCTTTGGCTGGCTCTGCATCATTTAGGACTAAAAAATCCAGAAAAGTCCCGAGTACCGACTAATTTTCCATCAACAATAAGGCTCCAGTTATTGCAACTAGCTTCGTTTTAAACAGTATCAGAGCTCTCTAATAATGTAGAAGTGATATAAGGTTGAGTATTTACTTTTGTAGAAGTTTGAACAATGATTTTTCACTTTTATTTTCTTTGTTTCAAACTGAGAGTGAGAGTAAGAGTGTGATTTGACTTGCTATGTTCACTGATGAGGGATTTTCTTTCTTTTTCTGCATACATTTTTATATAAAAG

mRNA sequence

CAAAAGGAAATTGGCAAGAAACAATGTTGTATTCAGGCTCTTCCATTTTGATTCTCTTACTTCCCTTTCTTCTCTCATTTTCAGTGCTTTCAGTCTCAGCTTCATTGGAGCAAAGTTTTCTACAATGTGTATCTCTCAATTCTCAACAATCTGTTCCTCCATTGTCTGTTTGTTCTCAGAACAATCCTTCATTCTTCCCTCTCCTTCAATCAACTGCACAAAACCTCAGGTTCTTGGACCCTTCAGTTCCCAAGCCTCAGTTTATCTTCACTCCATTGCATGACACTCATGTCCAAGCAGCGGTTATTTGTTCGAAGAAGCTCAACATACATCTTCGAGTTCGTAGTGGTGGCCATGACTATGAGGGTCTTTCCTACGCTTCCGAAACCGAAACACCTTTCATTGTCATAGACCTAGCGAAACTCAGGTCGGTGGAAGTGAATATCCAAGATAACACTGCATGGGCTCAGGCTGGTGCAACGGTTGGTGAAGTTTACTATAGAATCTCACAGAAAAGCAATGTCCATGGCTTCCCTGCTGGCCTTTGCACTAGCTTAGGCATTGGTGGCCACATAACAGGCGGTGCATATGGGTCCATGATGAGAAAATATGGCCTTGGTGCTGATAATGTCATTGATGCTCGGATTGTCGATGTTAATGGTAGAATCCTCGATAGATCCACGATGGGAGAGGACTACTTTTGGGCCATTAGAGGGGGTGCTGGAGGTAGCTTTGGGATTATTCTTTGGTGGAAGTTGCAATTGGTTCCTGTTCCAGAAACTGTGACAGTTTTCACTGTTCCAAAGACATTAGAGCAAGGTGCCACTCAAATCCTGTACAAATGGCAACAAGTTGCTGACAAGCTGGATGAAGATTTGTTCATTAGAGTCATAATACAAGTAACAAATGATAAAGCTACGAAAGAAAGAACCATTACCACAGCTTACAATGCTCTCTTTCTTGGAGATTCCGACAGACTCCTACAAATTATGAGAAAAAGCTTCCCTGAATTGGGTTTGACACCAAAAGATTGTATTGAAACCAACTGGATTAAATCAGTTCTTTACATAGCTGGAGAACCACCAAAAACACCACCTGAAGTTCTTCTACAAGGAAAGCCACAATTCAAGAACTACTTCAAAGCTAAATCAGACTTTGTTCAAGTCCCAATCCCAGAAACTGGATTGGAAGGTCTATGGAAAATATTTCTCCAAGAAGAAAGCCCATTGATGATCTGGAATCCTTATGGAGGAATGATGAGCAAGATCTCAGAGAATGAAATCCCATTCCCCCACAGAAAAGGAAACTTGTTCAAAATTCAGTATGTAAACCCATGGCAGGATGGAGATAAACACGAAACAGAACACATAGAGTGGATCAGAGAACTTTACAACTACATGGCTCCATATGTTTCCAAGTCTCCAAGAGCTGCATATGTAAACTACAGAGATCTTGATTTGGGTATGAACAAGGACAATACAAGCTTGGCTCATGCCACTGAATGGGGCAACAGATATTTCAAGAACAACTTCAATAGGCTTGTGAAGGTAAAGACAAAAGTTGATCCTGGCAACTTTTTCAGGCATGAACAGAGCATTCCTCCTTTGGCTGGCTCTGCATCATTTAGGACTAAAAAATCCAGAAAAGTCCCGAGTACCGACTAATTTTCCATCAACAATAAGGCTCCAGTTATTGCAACTAGCTTCGTTTTAAACAGTATCAGAGCTCTCTAATAATGTAGAAGTGATATAAGGTTGAGTATTTACTTTTGTAGAAGTTTGAACAATGATTTTTCACTTTTATTTTCTTTGTTTCAAACTGAGAGTGAGAGTAAGAGTGTGATTTGACTTGCTATGTTCACTGATGAGGGATTTTCTTTCTTTTTCTGCATACATTTTTATATAAAAG

Coding sequence (CDS)

ATGTTGTATTCAGGCTCTTCCATTTTGATTCTCTTACTTCCCTTTCTTCTCTCATTTTCAGTGCTTTCAGTCTCAGCTTCATTGGAGCAAAGTTTTCTACAATGTGTATCTCTCAATTCTCAACAATCTGTTCCTCCATTGTCTGTTTGTTCTCAGAACAATCCTTCATTCTTCCCTCTCCTTCAATCAACTGCACAAAACCTCAGGTTCTTGGACCCTTCAGTTCCCAAGCCTCAGTTTATCTTCACTCCATTGCATGACACTCATGTCCAAGCAGCGGTTATTTGTTCGAAGAAGCTCAACATACATCTTCGAGTTCGTAGTGGTGGCCATGACTATGAGGGTCTTTCCTACGCTTCCGAAACCGAAACACCTTTCATTGTCATAGACCTAGCGAAACTCAGGTCGGTGGAAGTGAATATCCAAGATAACACTGCATGGGCTCAGGCTGGTGCAACGGTTGGTGAAGTTTACTATAGAATCTCACAGAAAAGCAATGTCCATGGCTTCCCTGCTGGCCTTTGCACTAGCTTAGGCATTGGTGGCCACATAACAGGCGGTGCATATGGGTCCATGATGAGAAAATATGGCCTTGGTGCTGATAATGTCATTGATGCTCGGATTGTCGATGTTAATGGTAGAATCCTCGATAGATCCACGATGGGAGAGGACTACTTTTGGGCCATTAGAGGGGGTGCTGGAGGTAGCTTTGGGATTATTCTTTGGTGGAAGTTGCAATTGGTTCCTGTTCCAGAAACTGTGACAGTTTTCACTGTTCCAAAGACATTAGAGCAAGGTGCCACTCAAATCCTGTACAAATGGCAACAAGTTGCTGACAAGCTGGATGAAGATTTGTTCATTAGAGTCATAATACAAGTAACAAATGATAAAGCTACGAAAGAAAGAACCATTACCACAGCTTACAATGCTCTCTTTCTTGGAGATTCCGACAGACTCCTACAAATTATGAGAAAAAGCTTCCCTGAATTGGGTTTGACACCAAAAGATTGTATTGAAACCAACTGGATTAAATCAGTTCTTTACATAGCTGGAGAACCACCAAAAACACCACCTGAAGTTCTTCTACAAGGAAAGCCACAATTCAAGAACTACTTCAAAGCTAAATCAGACTTTGTTCAAGTCCCAATCCCAGAAACTGGATTGGAAGGTCTATGGAAAATATTTCTCCAAGAAGAAAGCCCATTGATGATCTGGAATCCTTATGGAGGAATGATGAGCAAGATCTCAGAGAATGAAATCCCATTCCCCCACAGAAAAGGAAACTTGTTCAAAATTCAGTATGTAAACCCATGGCAGGATGGAGATAAACACGAAACAGAACACATAGAGTGGATCAGAGAACTTTACAACTACATGGCTCCATATGTTTCCAAGTCTCCAAGAGCTGCATATGTAAACTACAGAGATCTTGATTTGGGTATGAACAAGGACAATACAAGCTTGGCTCATGCCACTGAATGGGGCAACAGATATTTCAAGAACAACTTCAATAGGCTTGTGAAGGTAAAGACAAAAGTTGATCCTGGCAACTTTTTCAGGCATGAACAGAGCATTCCTCCTTTGGCTGGCTCTGCATCATTTAGGACTAAAAAATCCAGAAAAGTCCCGAGTACCGACTAA

Protein sequence

MLYSGSSILILLLPFLLSFSVLSVSASLEQSFLQCVSLNSQQSVPPLSVCSQNNPSFFPLLQSTAQNLRFLDPSVPKPQFIFTPLHDTHVQAAVICSKKLNIHLRVRSGGHDYEGLSYASETETPFIVIDLAKLRSVEVNIQDNTAWAQAGATVGEVYYRISQKSNVHGFPAGLCTSLGIGGHITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDRSTMGEDYFWAIRGGAGGSFGIILWWKLQLVPVPETVTVFTVPKTLEQGATQILYKWQQVADKLDEDLFIRVIIQVTNDKATKERTITTAYNALFLGDSDRLLQIMRKSFPELGLTPKDCIETNWIKSVLYIAGEPPKTPPEVLLQGKPQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISENEIPFPHRKGNLFKIQYVNPWQDGDKHETEHIEWIRELYNYMAPYVSKSPRAAYVNYRDLDLGMNKDNTSLAHATEWGNRYFKNNFNRLVKVKTKVDPGNFFRHEQSIPPLAGSASFRTKKSRKVPSTD*

Homology

[Close]

BLAST of CSPI01G24800 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 711.8 bits (1836), Expect = 5.8e-204
Identity = 346/530 (65.28%), Postives = 423/530 (79.81%), Query Frame = 0


Query: 1   MLYSGSSILILLLPFLLSFSVLSVSASLEQSFLQCVSLNSQQSVPPLS---VCSQNNPSF 60
           ++ + ++ L+ LL   LS+  LS S +++Q F+ C+  NS  S P  S       N  SF
Sbjct: 5   LMNNTNAFLVTLLLLSLSYIPLSFS-TIQQDFVMCLVDNSDASFPMDSSFFTHDLNASSF 64

Query: 61  FPLLQSTAQNLRFLDPSVPKPQFIFTPLHDTHVQAAVICSKKLNIHLRVRSGGHDYEGLS 120
              L+++AQNLR+L PS PKP+FIF PL++THVQAAV+C+KKL +HLR+RSGGHDYEGLS
Sbjct: 65  KLALETSAQNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLS 124

Query: 121 YASETETPFIVIDLAKLRSVEVNIQDNTAWAQAGATVGEVYYRISQKSNVHGFPAGLCTS 180
           Y SE ET F+++DL+KLR + V+I+ N+AW  AGA++GEVYYRI +KS +HGFPAGLCTS
Sbjct: 125 YVSEMETAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTS 184

Query: 181 LGIGGHITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDRSTMGEDYFWAIRGGAGGSF 240
           LGIGGHI GGAYGSMMRK+GLGADNV+DARIVD +G+IL+R+ MGED FWAIRGG GGSF
Sbjct: 185 LGIGGHIIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSF 244

Query: 241 GIILWWKLQLVPVPETVTVFTVPKTLEQGATQILYKWQQVADKLDEDLFIRVIIQVTN-D 300
           G+IL WK++LVPVPE VTVFTV +TLEQ  T++LYKWQQVADKLDEDLFIRVIIQ T+  
Sbjct: 245 GVILAWKIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKT 304

Query: 301 KATKERTITTAYNALFLGDSDRLLQIMRKSFPELGLTPKDCIETNWIKSVLYIAGEPPKT 360
             +KERTI+T+Y   FLGD++RLLQ+M++SFP+LGLT KDC+ET+WIKSV+YIAG P   
Sbjct: 305 PKSKERTISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTA 364

Query: 361 PPEVLLQGKPQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKIS 420
           P E LL GK  FKNYFKAKSD+V+ PIP  GLEGLW+  L+E+SPL IWNPYGGMM+KI 
Sbjct: 365 PSEALLDGKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWNPYGGMMAKIP 424

Query: 421 ENEIPFPHRKGNLFKIQYVNPWQDGDKHETEHIEWIRELYNYMAPYVSKSPRAAYVNYRD 480
           E E PFPHR G LFKIQ++  WQDG   E +H+ W+RE+Y+YM  YVSKSPR+AYVNYRD
Sbjct: 425 ETETPFPHRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRD 484

Query: 481 LDLGMNKDNTSLAHATEWGNRYFKNNFNRLVKVKTKVDPGNFFRHEQSIP 527
           LDLGMN   +    A EWGNRYFK NF RLV++K K DP NFFRHEQSIP
Sbjct: 485 LDLGMNGKGSD---AREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIP 530

[Close]

BLAST of CSPI01G24800 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 711.8 bits (1836), Expect = 5.8e-204
Identity = 347/525 (66.10%), Postives = 417/525 (79.43%), Query Frame = 0


Query: 11  LLLPFLLSFSVLSVSASLEQSFLQCVSLNSQQSVPPLSVC---SQNNPSFFPLLQSTAQN 70
           L L  LL  SV   S++L+Q F++C+  NS  S P  +      QN   F   L+STAQN
Sbjct: 12  LFLVTLLLISVPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTAQN 71

Query: 71  LRFLDPSVPKPQFIFTPLHDTHVQAAVICSKKLNIHLRVRSGGHDYEGLSYASETETPFI 130
           LR+L PS PKP FIF PL++THVQAAV+C+KKL +HLR+RSGGHDYEGLS+ +E ETPF+
Sbjct: 72  LRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFV 131

Query: 131 VIDLAKLRSVEVNIQDNTAWAQAGATVGEVYYRISQKSNVHGFPAGLCTSLGIGGHITGG 190
           ++DL+KLR V+V++  N+AWA AGAT+GEVYYRI +KS  HGFPAGLC+SLGIGGH+ GG
Sbjct: 132 IVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGG 191

Query: 191 AYGSMMRKYGLGADNVIDARIVDVNGRILDRSTMGEDYFWAIRGGAGGSFGIILWWKLQL 250
           AYGSMMRK+GLGADNV+DARIVD NG+ILDR+ MGED FWAIRGG GGSFG+IL WK++L
Sbjct: 192 AYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKL 251

Query: 251 VPVPETVTVFTVPKTLEQGATQILYKWQQVADKLDEDLFIRVIIQVTNDKATK--ERTIT 310
           VPVP TVTVFTV KTLEQ  T++LYKW+Q+ADKLD+DLFIRVII   + K TK   RTI+
Sbjct: 252 VPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPAS-KTTKPGNRTIS 311

Query: 311 TAYNALFLGDSDRLLQIMRKSFPELGLTPKDCIETNWIKSVLYIAGEPPKTPPEVLLQGK 370
            +Y A FLGDS+RLLQ+M+KSFPELGLT KDC E +WIKSV+YIAG P    PE LL GK
Sbjct: 312 MSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALLAGK 371

Query: 371 PQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISENEIPFPHR 430
             FKN+FKAKSDFV+ PIP  GLEGLW+ FL+E+SPL IWNPYGGMMS+ISE+EIPFPHR
Sbjct: 372 SLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGGMMSRISESEIPFPHR 431

Query: 431 KGNLFKIQYVNPWQDGDKHETEHIEWIRELYNYMAPYVSKSPRAAYVNYRDLDLGMNKDN 490
            G LFKIQ+++ WQDG   E  H++WIRE+Y+YM  YVSK+PR AYVNYRDLDLG N+  
Sbjct: 432 NGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNEGE 491

Query: 491 TSLAHATEWGNRYFKNNFNRLVKVKTKVDPGNFFRHEQSIPPLAG 531
           T    A EWG +Y+K NF RLVK+K + DP NFFRHEQS+P   G
Sbjct: 492 TD---AREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPTKIG 532

[Close]

BLAST of CSPI01G24800 vs. ExPASy Swiss-Prot
Match: Q9FKU8 (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 4.8e-198
Identity = 342/529 (64.65%), Postives = 416/529 (78.64%), Query Frame = 0


Query: 7   SILILLLPFLLSFSVLSVSASLEQSFLQCVSLNSQQSVP---PLSVCSQNNPSFFPLLQS 66
           SIL+L    L + +  S  ASL+  F+ CV  N+    P        ++N   F  +L+S
Sbjct: 11  SILVLYFS-LYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLES 70

Query: 67  TAQNLRFLDPSVPKPQFIFTPLHDTHVQAAVICSKKLNIHLRVRSGGHDYEGLSYASETE 126
           TAQNLRFL  S+PKP FIF+P+H++HVQA++ICSKKL +HLRVRSGGHDYEGLSY S+ +
Sbjct: 71  TAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQID 130

Query: 127 TPFIVIDLAKLRSVEVNIQDNTAWAQAGATVGEVYYRISQKSNVHGFPAGLCTSLGIGGH 186
            PFI++DL+K+R V +NIQDN+AW Q+GATVGE+YYRI++KS VHGFPAGLC+SLGIGGH
Sbjct: 131 KPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGH 190

Query: 187 ITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDRSTMGEDYFWAIRGGAGGSFGIILWW 246
           ITGGAYGSMMRKYGLGADNV+DA+IVD NG++LDR+ MGED FWAIRGGAGGSFGIIL W
Sbjct: 191 ITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAW 250

Query: 247 KLQLVPVPETVTVFTVPKTLEQG-ATQILYKWQQVADKLDEDLFIRVIIQVTNDKATKER 306
           K++LVPVP+TVTVFTV KTL+Q    +I+ KWQ+VADKL E+LFIRV+  V      K  
Sbjct: 251 KIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTGGNK-- 310

Query: 307 TITTAYNALFLGDSDRLLQIMRKSFPELGLTPKDCIETNWIKSVLYIAGEPPKTPPEVLL 366
           T+TT+YNALFLG    L+ +M+KSFPELGLT KDCIE +W++S+ YI+G P  TP  VLL
Sbjct: 311 TVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLL 370

Query: 367 QGKPQF-KNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISENEIP 426
           QGK  F K  FKAKSDFV+ PIPE+GL+G++K  L+E+ PLMIWNPYGGMM+KI E++IP
Sbjct: 371 QGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPLMIWNPYGGMMAKIPESQIP 430

Query: 427 FPHRKGNLFKIQYVNPWQDGDKHETEHIEWIRELYNYMAPYVSKSPRAAYVNYRDLDLGM 486
           FPHRKG LFK+QYV  W D DK  + HI WIR+LY+YM PYVS +PR AYVNYRDLDLG 
Sbjct: 431 FPHRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGR 490

Query: 487 N-KD-NTSLAHATEWGNRYFKNNFNRLVKVKTKVDPGNFFRHEQSIPPL 529
           N KD  T +  A  WG  YFKNNFNRL+ +K KVDP NFFRHEQSIPP+
Sbjct: 491 NTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536

[Close]

BLAST of CSPI01G24800 vs. ExPASy Swiss-Prot
Match: Q9FKU9 (Berberine bridge enzyme-like 25 OS=Arabidopsis thaliana OX=3702 GN=At5g44390 PE=2 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 3.1e-181
Identity = 315/532 (59.21%), Postives = 397/532 (74.62%), Query Frame = 0


Query: 6   SSILILLLPFLLSFSVLSVSASLEQSFLQCV--SLNSQQSVPPLSVCSQNNPSFF-PLLQ 65
           S I +  L F      L+ S SL+  F++C+  + N + ++       + N S F  +L+
Sbjct: 11  SCISVFALYFSFYTITLTSSTSLQDDFIKCLYRNTNVRFTLDKTFFTPERNASIFTEVLE 70

Query: 66  STAQNLRFLDPSVPKPQFIFTPLHDTHVQAAVICSKKLNIHLRVRSGGHDYEGLSYASET 125
           STAQN R+L  ++PKP FIF P+H++HVQA+VICSKKL IH RVRSGGHDYEG+SY S+ 
Sbjct: 71  STAQNQRYLTKTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYEGVSYVSQI 130

Query: 126 ETPFIVIDLAKLRSVEVNIQDNTAWAQAGATVGEVYYRISQKSNVHGFPAGLCTSLGIGG 185
           E PF++IDL+KLR + V+I+D +AW +AGATVGE+YYRI++KS  HGFPAG+  SLGIGG
Sbjct: 131 EKPFVLIDLSKLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPAGVYPSLGIGG 190

Query: 186 HITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDRSTMGEDYFWAIRGGAGGSFGIILW 245
           HITGGAYGS+MRKYGL ADNV+DA+IVD NG++LDR++MGED FWAIRGG+GGSFGIIL 
Sbjct: 191 HITGGAYGSLMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGIILS 250

Query: 246 WKLQLVPVPETVTVFTVPKTLEQGAT-QILYKWQQVADKLDEDLFIRVIIQVTNDKATKE 305
           WK++LVPVPET+TVFTV KT EQ  + +IL KWQ++AD L ++LF+RV   V+ +KA K 
Sbjct: 251 WKIKLVPVPETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFTVSGNKANK- 310

Query: 306 RTITTAYNALFLGDSDRLLQIMRKSFPELGLTPKDCIETNWIKSVLYIAGEPPKTPP--E 365
            T+T AY   FLG+   L+++M+K FPELGLT KDCIE +WI S++Y +G P   PP  E
Sbjct: 311 -TVTMAYIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFPTNPPPPIE 370

Query: 366 VLLQGK-PQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISEN 425
           +LLQ K P  K YFK KSDF + PIP  GLEG++K  L+E++ L+IW PYGG M KI E+
Sbjct: 371 ILLQAKSPIGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEEDAALVIWTPYGGKMDKIPES 430

Query: 426 EIPFPHRKGNLFKIQYVNPWQDGDKHETEHIEWIRELYNYMAPYVSKSPRAAYVNYRDLD 485
           EIPFPHR G  F IQY   W D +K      +WIRELY YM PYVS +PR AYVNYRDLD
Sbjct: 431 EIPFPHRNGTNFMIQYYRSWSDSEKRPNRRTKWIRELYGYMTPYVSSNPRQAYVNYRDLD 490

Query: 486 LGMNKDN--TSLAHATEWGNRYFKNNFNRLVKVKTKVDPGNFFRHEQSIPPL 529
           LG NKDN  ++   A  WG  YFK+NFNRLV++K+KVDP NFFRHEQSIP L
Sbjct: 491 LGQNKDNSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPTL 540

[Close]

BLAST of CSPI01G24800 vs. ExPASy Swiss-Prot
Match: Q9FKV0 (Berberine bridge enzyme-like 24 OS=Arabidopsis thaliana OX=3702 GN=At5g44380 PE=2 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 4.8e-174
Identity = 299/531 (56.31%), Postives = 389/531 (73.26%), Query Frame = 0


Query: 6   SSILILLLPF-LLSFSVLSVSASLEQSFLQCVSLNSQQSVP---PLSVCSQNNPSFFPLL 65
           S I++ +L F     +  S SAS++  F+ CV  N+  S P    L   ++N   F  +L
Sbjct: 11  SCIIVSVLYFSFYCITPTSSSASIQDQFINCVKRNTHVSFPLEKTLFTPAKNVSLFNQVL 70

Query: 66  QSTAQNLRFLDPSVPKPQFIFTPLHDTHVQAAVICSKKLNIHLRVRSGGHDYEGLSYASE 125
           +STAQNL+FL  S+PKP FIF P+H + VQA++ICSKKL IH RVRSGGHD+E LSY S 
Sbjct: 71  ESTAQNLQFLAKSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYVSR 130

Query: 126 TETPFIVIDLAKLRSVEVNIQDNTAWAQAGATVGEVYYRISQKSNVHGFPAGLCTSLGIG 185
            E PFI++DL+KL+ + V+I+ N+AW Q GAT+GE+YYRI++KS +HGFPAGLCTS+GIG
Sbjct: 131 IEKPFILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIG 190

Query: 186 GHITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDRSTMGEDYFWAIRGGAGGSFGIIL 245
           G++TGG YG++MRKYGL  DNV+D ++VD NG++LDR+ MGED FWAIRGG G SFGI+L
Sbjct: 191 GYMTGGGYGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVL 250

Query: 246 WWKLQLVPVPETVTVFTVPKTLEQGA-TQILYKWQQVADKLDEDLFIRVIIQVTNDKATK 305
            WK++LVPVP+TVTVFTV KTLEQ A  + + KWQQ++ K+ E++ IRV+++   +   K
Sbjct: 251 AWKIKLVPVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLRAAGNDGNK 310

Query: 306 ERTITTAYNALFLGDSDRLLQIMRKSFPELGLTPKDCIETNWIKSVLYIAGEPPKTPPEV 365
             T+T  Y   FLG+   LL++M K+FPELGLT KDC E +WI++ L+  G P  +P E+
Sbjct: 311 --TVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGGFPTGSPIEI 370

Query: 366 LLQGK-PQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISENE 425
           LLQ K P  K+YFKA SDFV+ PIP  GL+G++K  ++  +  + W PYGGMMSKI E+ 
Sbjct: 371 LLQLKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRLIEGNTTFLNWTPYGGMMSKIPESA 430

Query: 426 IPFPHRKGNLFKIQYVNPWQDGDKHETEHIEWIRELYNYMAPYVSKSPRAAYVNYRDLDL 485
           IPFPHR G LFKI Y   W + DK  +  I WI+E+YNYMAPYVS +PR AYVNYRDLD 
Sbjct: 431 IPFPHRNGTLFKILYYANWLENDKTSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRDLDF 490

Query: 486 GMNKDN--TSLAHATEWGNRYFKNNFNRLVKVKTKVDPGNFFRHEQSIPPL 529
           G NK+N   +   A  WG +YFK NF+RLVK+KTKVDP NFFRHEQSIPP+
Sbjct: 491 GQNKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPM 539

[Close]

BLAST of CSPI01G24800 vs. ExPASy TrEMBL
Match: A0A5A7V956 (Flavin-dependent oxidoreductase FOX5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold437G00120 PE=3 SV=1)

HSP 1 Score: 1045.8 bits (2703), Expect = 6.3e-302
Identity = 516/544 (94.85%), Postives = 528/544 (97.06%), Query Frame = 0


Query: 1   MLYSGSSILILLLPFLLSFSVLSVSASLEQSFLQCVSLNSQQSVPPLSVCSQNNPSFFPL 60
           MLYSG SILILLLPFLLS   LSVSASLEQSFLQCVSLNSQQSVPPLSVCSQNNPSFFPL
Sbjct: 1   MLYSGFSILILLLPFLLS---LSVSASLEQSFLQCVSLNSQQSVPPLSVCSQNNPSFFPL 60

Query: 61  LQSTAQNLRFLDPSVPKPQFIFTPLHDTHVQAAVICSKKLNIHLRVRSGGHDYEGLSYAS 120
           LQSTAQNLR+LDPSVPKPQFIFTPLHDTHVQAAVICSKKL IHLRVRSGGHDYEGLSYAS
Sbjct: 61  LQSTAQNLRYLDPSVPKPQFIFTPLHDTHVQAAVICSKKLQIHLRVRSGGHDYEGLSYAS 120

Query: 121 ETETPFIVIDLAKLRSVEVNIQDNTAWAQAGATVGEVYYRISQKSNVHGFPAGLCTSLGI 180
           E ETPFIVIDLAKLRSVEVNIQDNTAW QAGATVGEVYYRIS+KSNVHGFPAGLCTSLGI
Sbjct: 121 EIETPFIVIDLAKLRSVEVNIQDNTAWVQAGATVGEVYYRISEKSNVHGFPAGLCTSLGI 180

Query: 181 GGHITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDRSTMGEDYFWAIRGGAGGSFGII 240
           GGHITGGAYGSMMRKYGLGADNVIDARIVDVNGRIL+RSTMGEDYFWAIRGGAGGSFGII
Sbjct: 181 GGHITGGAYGSMMRKYGLGADNVIDARIVDVNGRILNRSTMGEDYFWAIRGGAGGSFGII 240

Query: 241 LWWKLQLVPVPETVTVFTVPKTLEQGATQILYKWQQVADKLDEDLFIRVIIQVTNDKATK 300
           LWWKLQLV VPE VTVFTVPKTL+QGATQ+LYKWQQVADKLDEDLFIRVIIQVTNDKATK
Sbjct: 241 LWWKLQLVSVPEIVTVFTVPKTLQQGATQVLYKWQQVADKLDEDLFIRVIIQVTNDKATK 300

Query: 301 ERTITTAYNALFLGDSDRLLQIMRKSFPELGLTPKDCIETNWIKSVLYIAGEPPKTPPEV 360
           ERTITTAYNALFLGDS+RLLQIMRKSFPELGLTPKDCIET+WIKSVLYIAGEPPKTPPEV
Sbjct: 301 ERTITTAYNALFLGDSNRLLQIMRKSFPELGLTPKDCIETSWIKSVLYIAGEPPKTPPEV 360

Query: 361 LLQGKPQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISENEI 420
           LLQGKPQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISENEI
Sbjct: 361 LLQGKPQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISENEI 420

Query: 421 PFPHRKGNLFKIQYVNPWQDGDKHETEHIEWIRELYNYMAPYVSKSPRAAYVNYRDLDLG 480
           PFPHRKGNLFKIQYVNPWQ GDKHET HIEWIRELYNYMAPYVSKSPRAAYVNYRDLDLG
Sbjct: 421 PFPHRKGNLFKIQYVNPWQAGDKHETRHIEWIRELYNYMAPYVSKSPRAAYVNYRDLDLG 480

Query: 481 MN-KDNTSLAHATEWGNRYFKNNFNRLVKVKTKVDPGNFFRHEQSIPPLAGSASFRTKKS 540
           MN KDNT+LA A EWGN+YFKNNFNRLVKVKTKVDPGNFFRHEQSIPPLA +ASFRTKKS
Sbjct: 481 MNKKDNTTLAQANEWGNKYFKNNFNRLVKVKTKVDPGNFFRHEQSIPPLASAASFRTKKS 540

Query: 541 RKVP 544
           ++ P
Sbjct: 541 KRSP 541

[Close]

BLAST of CSPI01G24800 vs. ExPASy TrEMBL
Match: A0A1S3BDU6 (flavin-dependent oxidoreductase FOX5-like OS=Cucumis melo OX=3656 GN=LOC103488583 PE=3 SV=1)

HSP 1 Score: 1045.8 bits (2703), Expect = 6.3e-302
Identity = 516/544 (94.85%), Postives = 528/544 (97.06%), Query Frame = 0


Query: 1   MLYSGSSILILLLPFLLSFSVLSVSASLEQSFLQCVSLNSQQSVPPLSVCSQNNPSFFPL 60
           MLYSG SILILLLPFLLS   LSVSASLEQSFLQCVSLNSQQSVPPLSVCSQNNPSFFPL
Sbjct: 1   MLYSGFSILILLLPFLLS---LSVSASLEQSFLQCVSLNSQQSVPPLSVCSQNNPSFFPL 60

Query: 61  LQSTAQNLRFLDPSVPKPQFIFTPLHDTHVQAAVICSKKLNIHLRVRSGGHDYEGLSYAS 120
           LQSTAQNLR+LDPSVPKPQFIFTPLHDTHVQAAVICSKKL IHLRVRSGGHDYEGLSYAS
Sbjct: 61  LQSTAQNLRYLDPSVPKPQFIFTPLHDTHVQAAVICSKKLQIHLRVRSGGHDYEGLSYAS 120

Query: 121 ETETPFIVIDLAKLRSVEVNIQDNTAWAQAGATVGEVYYRISQKSNVHGFPAGLCTSLGI 180
           E ETPFIVIDLAKLRSVEVNIQDNTAW QAGATVGEVYYRIS+KSNVHGFPAGLCTSLGI
Sbjct: 121 EIETPFIVIDLAKLRSVEVNIQDNTAWVQAGATVGEVYYRISEKSNVHGFPAGLCTSLGI 180

Query: 181 GGHITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDRSTMGEDYFWAIRGGAGGSFGII 240
           GGHITGGAYGSMMRKYGLGADNVIDARIVDVNGRIL+RSTMGEDYFWAIRGGAGGSFGII
Sbjct: 181 GGHITGGAYGSMMRKYGLGADNVIDARIVDVNGRILNRSTMGEDYFWAIRGGAGGSFGII 240

Query: 241 LWWKLQLVPVPETVTVFTVPKTLEQGATQILYKWQQVADKLDEDLFIRVIIQVTNDKATK 300
           LWWKLQLV VPE VTVFTVPKTL+QGATQ+LYKWQQVADKLDEDLFIRVIIQVTNDKATK
Sbjct: 241 LWWKLQLVSVPEIVTVFTVPKTLQQGATQVLYKWQQVADKLDEDLFIRVIIQVTNDKATK 300

Query: 301 ERTITTAYNALFLGDSDRLLQIMRKSFPELGLTPKDCIETNWIKSVLYIAGEPPKTPPEV 360
           ERTITTAYNALFLGDS+RLLQIMRKSFPELGLTPKDCIET+WIKSVLYIAGEPPKTPPEV
Sbjct: 301 ERTITTAYNALFLGDSNRLLQIMRKSFPELGLTPKDCIETSWIKSVLYIAGEPPKTPPEV 360

Query: 361 LLQGKPQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISENEI 420
           LLQGKPQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISENEI
Sbjct: 361 LLQGKPQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISENEI 420

Query: 421 PFPHRKGNLFKIQYVNPWQDGDKHETEHIEWIRELYNYMAPYVSKSPRAAYVNYRDLDLG 480
           PFPHRKGNLFKIQYVNPWQ GDKHET HIEWIRELYNYMAPYVSKSPRAAYVNYRDLDLG
Sbjct: 421 PFPHRKGNLFKIQYVNPWQAGDKHETRHIEWIRELYNYMAPYVSKSPRAAYVNYRDLDLG 480

Query: 481 MN-KDNTSLAHATEWGNRYFKNNFNRLVKVKTKVDPGNFFRHEQSIPPLAGSASFRTKKS 540
           MN KDNT+LA A EWGN+YFKNNFNRLVKVKTKVDPGNFFRHEQSIPPLA +ASFRTKKS
Sbjct: 481 MNKKDNTTLAQANEWGNKYFKNNFNRLVKVKTKVDPGNFFRHEQSIPPLASAASFRTKKS 540

Query: 541 RKVP 544
           ++ P
Sbjct: 541 KRSP 541

[Close]

BLAST of CSPI01G24800 vs. ExPASy TrEMBL
Match: A0A6J1CFR3 (berberine bridge enzyme-like 13 OS=Momordica charantia OX=3673 GN=LOC111010807 PE=3 SV=1)

HSP 1 Score: 932.6 bits (2409), Expect = 7.7e-268
Identity = 462/540 (85.56%), Postives = 491/540 (90.93%), Query Frame = 0


Query: 4   SGSSILILLLPFLLSFSVLSVSASLEQSFLQCVSLNSQQSVPPLSVCSQNNPSFFPLLQS 63
           SGS I  L+L FLLSFS LS SA LEQ+F QC+S+NSQ S PP SVC  NN SF  LLQS
Sbjct: 5   SGSPIFHLVLAFLLSFS-LSNSAPLEQAFFQCLSVNSQLSAPPFSVCDPNNASFTTLLQS 64

Query: 64  TAQNLRFLDPSVPKPQFIFTPLHDTHVQAAVICSKKLNIHLRVRSGGHDYEGLSYASETE 123
           TAQNLR+LD SVPKP+FIF PL D+HVQAAVICSKKL IHLRVRSGGHDYEGLSYASE E
Sbjct: 65  TAQNLRYLDASVPKPEFIFIPLLDSHVQAAVICSKKLRIHLRVRSGGHDYEGLSYASEIE 124

Query: 124 TPFIVIDLAKLRSVEVNIQDNTAWAQAGATVGEVYYRISQKSNVHGFPAGLCTSLGIGGH 183
           TPFIV+DL KLRSVEVNI+DN+AW QAGATVGEVYYRIS+KS VHGFPAGLCTSLGIGGH
Sbjct: 125 TPFIVVDLTKLRSVEVNIEDNSAWVQAGATVGEVYYRISEKSEVHGFPAGLCTSLGIGGH 184

Query: 184 ITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDRSTMGEDYFWAIRGGAGGSFGIILWW 243
           ITGGAYGSMMRKYGLGADNVIDARIVD+NGRILDR+ MGED+FWAIRGGAGGSFGIILWW
Sbjct: 185 ITGGAYGSMMRKYGLGADNVIDARIVDINGRILDRAAMGEDHFWAIRGGAGGSFGIILWW 244

Query: 244 KLQLVPVPETVTVFTVPKTLEQGATQILYKWQQVADKLDEDLFIRVIIQVTNDKATKERT 303
           KLQLVPVPETVTVFTVPKTLEQGATQ+LYKWQQVA KLDE+LFIRVIIQ T+DKATK+RT
Sbjct: 245 KLQLVPVPETVTVFTVPKTLEQGATQVLYKWQQVAHKLDENLFIRVIIQPTSDKATKKRT 304

Query: 304 ITTAYNALFLGDSDRLLQIMRKSFPELGLTPKDCIETNWIKSVLYIAGEPPKTPPEVLLQ 363
           ITTAYNALFLGDS+RLLQ+M KSFPELGLTPKDCIET+WIKSVLYIAGEPP TPPEVLLQ
Sbjct: 305 ITTAYNALFLGDSNRLLQVMGKSFPELGLTPKDCIETSWIKSVLYIAGEPPGTPPEVLLQ 364

Query: 364 GKPQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISENEIPFP 423
           GKPQFKNYFKAKSDFVQVPIPE GLEGLWK  L E+ PL IWNPYGGMMSKISENEIPFP
Sbjct: 365 GKPQFKNYFKAKSDFVQVPIPEAGLEGLWKRLLVEDKPLTIWNPYGGMMSKISENEIPFP 424

Query: 424 HRKGNLFKIQYVNPWQDGDKHETEHIEWIRELYNYMAPYVSKSPRAAYVNYRDLDLGMN- 483
           HRKGNLFKIQY++ WQ GD    +HIEWIRELYNYMAPYVS SPRAAYVNYRDLDLGMN 
Sbjct: 425 HRKGNLFKIQYLSTWQGGDTDAAKHIEWIRELYNYMAPYVSTSPRAAYVNYRDLDLGMNK 484

Query: 484 KDNTSLAHATEWGNRYFKNNFNRLVKVKTKVDPGNFFRHEQSIPPLAGSASF-RTKKSRK 542
           KDNTSL  ATEWGN+YFK+NFNRLVKVKTKVDP NFFRHEQSIPPLAG+ASF RT K R+
Sbjct: 485 KDNTSLVQATEWGNKYFKDNFNRLVKVKTKVDPENFFRHEQSIPPLAGTASFKRTTKYRR 543

[Close]

BLAST of CSPI01G24800 vs. ExPASy TrEMBL
Match: A0A6J1K3T7 (berberine bridge enzyme-like 13 OS=Cucurbita maxima OX=3661 GN=LOC111490401 PE=3 SV=1)

HSP 1 Score: 924.9 bits (2389), Expect = 1.6e-265
Identity = 460/546 (84.25%), Postives = 494/546 (90.48%), Query Frame = 0


Query: 1   MLYSGSSILILLLP---FLLSFSVLSVSASLEQSFLQCVSLNSQQSVPPLSVCSQNNPSF 60
           ML S SSIL LLL    FLLS SV S SA LEQSFL C+S NSQQSVPP SVC+  N SF
Sbjct: 1   MLNSTSSILPLLLTLLVFLLSVSV-SNSALLEQSFLHCLSTNSQQSVPPFSVCAPTNASF 60

Query: 61  FPLLQSTAQNLRFLDPSVPKPQFIFTPLHDTHVQAAVICSKKLNIHLRVRSGGHDYEGLS 120
            PLLQSTAQNLR+ DPSVPKP+FIFTPL+DTHVQAAV+CSK L IHLRVRSGGHDYEGLS
Sbjct: 61  IPLLQSTAQNLRYSDPSVPKPEFIFTPLYDTHVQAAVVCSKGLQIHLRVRSGGHDYEGLS 120

Query: 121 YASETETPFIVIDLAKLRSVEVNIQDNTAWAQAGATVGEVYYRISQKSNVHGFPAGLCTS 180
           Y S+ ETPFIV+DLAKLRSVEVNI+DNTAW QAGATVGEVYYRIS+KSNVHGFPAGLCTS
Sbjct: 121 YVSQIETPFIVVDLAKLRSVEVNIEDNTAWVQAGATVGEVYYRISEKSNVHGFPAGLCTS 180

Query: 181 LGIGGHITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDRSTMGEDYFWAIRGGAGGSF 240
           LGIGGHITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDR  MGED+FWAIRGGAGGSF
Sbjct: 181 LGIGGHITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDRKAMGEDHFWAIRGGAGGSF 240

Query: 241 GIILWWKLQLVPVPETVTVFTVPKTLEQGATQILYKWQQVADKLDEDLFIRVIIQVTNDK 300
           GIILWWKL+LVPVPETVTVFTVP+TLEQGATQILYKWQQ+ADKLDEDLFIRVIIQV++DK
Sbjct: 241 GIILWWKLRLVPVPETVTVFTVPRTLEQGATQILYKWQQIADKLDEDLFIRVIIQVSSDK 300

Query: 301 ATKERTITTAYNALFLGDSDRLLQIMRKSFPELGLTPKDCIETNWIKSVLYIAGEPPKTP 360
            TK+RTITTAYNALFLGDS+RLLQ+M KSFPELGLT KDC ET+WIKSVLYIAGE P+TP
Sbjct: 301 TTKQRTITTAYNALFLGDSNRLLQVMGKSFPELGLTSKDCTETSWIKSVLYIAGEAPRTP 360

Query: 361 PEVLLQGKPQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISE 420
           PEVLLQGKPQFK+YFKAKSDFV VPIPE GLEGLWK  L ++ P MIWNPYGGMMSKISE
Sbjct: 361 PEVLLQGKPQFKSYFKAKSDFVHVPIPEVGLEGLWKKLLLDDMPFMIWNPYGGMMSKISE 420

Query: 421 NEIPFPHRKGNLFKIQYVNPWQDGDKHETEHIEWIRELYNYMAPYVSKSPRAAYVNYRDL 480
           NEIPFPHRKGNLFKIQY+  WQ G+K   +HIEWIRELYNYMAPYVSKSPRAAYVNYRDL
Sbjct: 421 NEIPFPHRKGNLFKIQYLTSWQGGNKDVEKHIEWIRELYNYMAPYVSKSPRAAYVNYRDL 480

Query: 481 DLGMN-KDNTSLAHATEWGNRYFKNNFNRLVKVKTKVDPGNFFRHEQSIPPLAGSASFR- 540
           DLGMN K+NTSLA A+EWGN+YFK+NFNRL+KVKTKVDP NFFRHEQSIPP A + SFR 
Sbjct: 481 DLGMNKKNNTSLAQASEWGNKYFKDNFNRLIKVKTKVDPDNFFRHEQSIPPFAVTKSFRK 540

Query: 541 TKKSRK 542
           T+K RK
Sbjct: 541 TRKFRK 545

[Close]

BLAST of CSPI01G24800 vs. ExPASy TrEMBL
Match: A0A6J1EJU9 (berberine bridge enzyme-like 13 OS=Cucurbita moschata OX=3662 GN=LOC111433979 PE=3 SV=1)

HSP 1 Score: 921.4 bits (2380), Expect = 1.8e-264
Identity = 455/542 (83.95%), Postives = 496/542 (91.51%), Query Frame = 0


Query: 1   MLYSGSSILILLLPFLLSFSVLSVSASLEQSFLQCVSLNSQQSVPPLSVCSQNNPSFFPL 60
           M++SGSS + LLL  LLS S LS SAS+EQSF+QC+++NSQQ++PP SVC+QNN SFF L
Sbjct: 1   MVHSGSS-MFLLLASLLSLS-LSSSASMEQSFVQCLAMNSQQTIPPFSVCAQNNASFFTL 60

Query: 61  LQSTAQNLRFLDPSVPKPQFIFTPLHDTHVQAAVICSKKLNIHLRVRSGGHDYEGLSYAS 120
           LQSTAQNLR+LDPS+PKP+FIFTPLHDTHVQAAVICS KL IHLRVRSGGHDYEGLSYAS
Sbjct: 61  LQSTAQNLRYLDPSLPKPEFIFTPLHDTHVQAAVICSNKLRIHLRVRSGGHDYEGLSYAS 120

Query: 121 ETETPFIVIDLAKLRSVEVNIQDNTAWAQAGATVGEVYYRISQKSNVHGFPAGLCTSLGI 180
           E  TPF+V+DLAKLRSV+VNIQDNTAW QAGATVGEVYYRIS+KSNVHGFPAGLCTSLGI
Sbjct: 121 EIGTPFVVLDLAKLRSVQVNIQDNTAWVQAGATVGEVYYRISEKSNVHGFPAGLCTSLGI 180

Query: 181 GGHITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDRSTMGEDYFWAIRGGAGGSFGII 240
           GGHITGGAYGSMMRKYGL ADNV+DARIVDVNGRILDRSTMGEDYFWAIRGGAGGSFGII
Sbjct: 181 GGHITGGAYGSMMRKYGLAADNVVDARIVDVNGRILDRSTMGEDYFWAIRGGAGGSFGII 240

Query: 241 LWWKLQLVPVPETVTVFTVPKTLEQGATQILYKWQQVADKLDEDLFIRVIIQVTNDKATK 300
           LWWKLQLVPVPETVTVFTVPK LEQGATQILYKWQQ+ADKLDEDLFIRVIIQ T DK TK
Sbjct: 241 LWWKLQLVPVPETVTVFTVPKILEQGATQILYKWQQIADKLDEDLFIRVIIQATIDKPTK 300

Query: 301 ERTITTAYNALFLGDSDRLLQIMRKSFPELGLTPKDCIETNWIKSVLYIAGEPPKTPPEV 360
           +RTI+TAYNALFLGDS  LLQ+M KSFPELGLTPKDCIET+WIKSVLYIAGE  KTPPEV
Sbjct: 301 KRTISTAYNALFLGDSKTLLQVMEKSFPELGLTPKDCIETSWIKSVLYIAGESIKTPPEV 360

Query: 361 LLQGKPQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISENEI 420
           LLQGKPQFKNYFKAKSDFVQ+PIPETGLEGLWK  L E+SPLMI NPYGGMMSKISE+EI
Sbjct: 361 LLQGKPQFKNYFKAKSDFVQIPIPETGLEGLWKRLLVEDSPLMILNPYGGMMSKISESEI 420

Query: 421 PFPHRKGNLFKIQYVNPWQDGDKHETEHIEWIRELYNYMAPYVSKSPRAAYVNYRDLDLG 480
           PFPHRKGNLFKIQY++ WQ G++H  +HIEWIRELYNYMAPYVSK+PRAAYVNYRDLDLG
Sbjct: 421 PFPHRKGNLFKIQYLSLWQGGNRHAAKHIEWIRELYNYMAPYVSKAPRAAYVNYRDLDLG 480

Query: 481 MN-KDNTSLAHATEWGNRYFKNNFNRLVKVKTKVDPGNFFRHEQSIPPLAGSASFRTKKS 540
           MN K+N SLA A+EWG++YFK+NFNRLV++KTKVDP NFF HEQSIP L      RTKK 
Sbjct: 481 MNMKNNMSLAEASEWGSKYFKDNFNRLVEIKTKVDPDNFFWHEQSIPSLG-----RTKKI 535

Query: 541 RK 542
           +K
Sbjct: 541 QK 535

[Close]

BLAST of CSPI01G24800 vs. NCBI nr
Match: XP_004135540.1 (berberine bridge enzyme-like 15 [Cucumis sativus] >KGN66361.2 hypothetical protein Csa_023334 [Cucumis sativus])

HSP 1 Score: 1109.0 bits (2867), Expect = 0.0e+00
Identity = 545/546 (99.82%), Postives = 546/546 (100.00%), Query Frame = 0


Query: 1   MLYSGSSILILLLPFLLSFSVLSVSASLEQSFLQCVSLNSQQSVPPLSVCSQNNPSFFPL 60
           MLYSGSSILILLLPFLLSFSVLSVSASLEQSFLQCVSLNSQQSVPPLSVCSQNNPSFFPL
Sbjct: 1   MLYSGSSILILLLPFLLSFSVLSVSASLEQSFLQCVSLNSQQSVPPLSVCSQNNPSFFPL 60

Query: 61  LQSTAQNLRFLDPSVPKPQFIFTPLHDTHVQAAVICSKKLNIHLRVRSGGHDYEGLSYAS 120
           LQSTAQNLRFLDPSVPKPQFIFTPLHDTHVQAAVICSKKLNIHLRVRSGGHDYEGLSYAS
Sbjct: 61  LQSTAQNLRFLDPSVPKPQFIFTPLHDTHVQAAVICSKKLNIHLRVRSGGHDYEGLSYAS 120

Query: 121 ETETPFIVIDLAKLRSVEVNIQDNTAWAQAGATVGEVYYRISQKSNVHGFPAGLCTSLGI 180
           ETETPFIVIDLAKLRSVEVNIQDNTAWAQAGATVGEVYYRISQKSNVHGFPAGLCTSLGI
Sbjct: 121 ETETPFIVIDLAKLRSVEVNIQDNTAWAQAGATVGEVYYRISQKSNVHGFPAGLCTSLGI 180

Query: 181 GGHITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDRSTMGEDYFWAIRGGAGGSFGII 240
           GGHITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDRSTMGEDYFWAIRGGAGGSFGII
Sbjct: 181 GGHITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDRSTMGEDYFWAIRGGAGGSFGII 240

Query: 241 LWWKLQLVPVPETVTVFTVPKTLEQGATQILYKWQQVADKLDEDLFIRVIIQVTNDKATK 300
           LWWKLQLVPVPETVTVFTVPKTLEQGATQILYKWQQVADKLDEDLFIRVIIQVTNDKATK
Sbjct: 241 LWWKLQLVPVPETVTVFTVPKTLEQGATQILYKWQQVADKLDEDLFIRVIIQVTNDKATK 300

Query: 301 ERTITTAYNALFLGDSDRLLQIMRKSFPELGLTPKDCIETNWIKSVLYIAGEPPKTPPEV 360
           ERTITTAYNALFLGDSDRLLQIMRKSFPELGLTPKDCIETNWIKSVLYIAGEPPKTPPEV
Sbjct: 301 ERTITTAYNALFLGDSDRLLQIMRKSFPELGLTPKDCIETNWIKSVLYIAGEPPKTPPEV 360

Query: 361 LLQGKPQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISENEI 420
           LLQGKPQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISENEI
Sbjct: 361 LLQGKPQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISENEI 420

Query: 421 PFPHRKGNLFKIQYVNPWQDGDKHETEHIEWIRELYNYMAPYVSKSPRAAYVNYRDLDLG 480
           PFPHRKGNLFKIQYVNPWQDGDKHETEHIEWIRELYNYMAPYVSKSPRAAYVNYRDLDLG
Sbjct: 421 PFPHRKGNLFKIQYVNPWQDGDKHETEHIEWIRELYNYMAPYVSKSPRAAYVNYRDLDLG 480

Query: 481 MNKDNTSLAHATEWGNRYFKNNFNRLVKVKTKVDPGNFFRHEQSIPPLAGSASFRTKKSR 540
           MNKDNTSLAHATEWGN+YFKNNFNRLVKVKTKVDPGNFFRHEQSIPPLAGSASFRTKKSR
Sbjct: 481 MNKDNTSLAHATEWGNKYFKNNFNRLVKVKTKVDPGNFFRHEQSIPPLAGSASFRTKKSR 540

Query: 541 KVPSTD 547
           KVPSTD
Sbjct: 541 KVPSTD 546

[Close]

BLAST of CSPI01G24800 vs. NCBI nr
Match: XP_008445619.2 (PREDICTED: flavin-dependent oxidoreductase FOX5-like [Cucumis melo] >KAA0063744.1 flavin-dependent oxidoreductase FOX5-like [Cucumis melo var. makuwa] >TYK14947.1 flavin-dependent oxidoreductase FOX5-like [Cucumis melo var. makuwa])

HSP 1 Score: 1045.8 bits (2703), Expect = 1.3e-301
Identity = 516/544 (94.85%), Postives = 528/544 (97.06%), Query Frame = 0


Query: 1   MLYSGSSILILLLPFLLSFSVLSVSASLEQSFLQCVSLNSQQSVPPLSVCSQNNPSFFPL 60
           MLYSG SILILLLPFLLS   LSVSASLEQSFLQCVSLNSQQSVPPLSVCSQNNPSFFPL
Sbjct: 1   MLYSGFSILILLLPFLLS---LSVSASLEQSFLQCVSLNSQQSVPPLSVCSQNNPSFFPL 60

Query: 61  LQSTAQNLRFLDPSVPKPQFIFTPLHDTHVQAAVICSKKLNIHLRVRSGGHDYEGLSYAS 120
           LQSTAQNLR+LDPSVPKPQFIFTPLHDTHVQAAVICSKKL IHLRVRSGGHDYEGLSYAS
Sbjct: 61  LQSTAQNLRYLDPSVPKPQFIFTPLHDTHVQAAVICSKKLQIHLRVRSGGHDYEGLSYAS 120

Query: 121 ETETPFIVIDLAKLRSVEVNIQDNTAWAQAGATVGEVYYRISQKSNVHGFPAGLCTSLGI 180
           E ETPFIVIDLAKLRSVEVNIQDNTAW QAGATVGEVYYRIS+KSNVHGFPAGLCTSLGI
Sbjct: 121 EIETPFIVIDLAKLRSVEVNIQDNTAWVQAGATVGEVYYRISEKSNVHGFPAGLCTSLGI 180

Query: 181 GGHITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDRSTMGEDYFWAIRGGAGGSFGII 240
           GGHITGGAYGSMMRKYGLGADNVIDARIVDVNGRIL+RSTMGEDYFWAIRGGAGGSFGII
Sbjct: 181 GGHITGGAYGSMMRKYGLGADNVIDARIVDVNGRILNRSTMGEDYFWAIRGGAGGSFGII 240

Query: 241 LWWKLQLVPVPETVTVFTVPKTLEQGATQILYKWQQVADKLDEDLFIRVIIQVTNDKATK 300
           LWWKLQLV VPE VTVFTVPKTL+QGATQ+LYKWQQVADKLDEDLFIRVIIQVTNDKATK
Sbjct: 241 LWWKLQLVSVPEIVTVFTVPKTLQQGATQVLYKWQQVADKLDEDLFIRVIIQVTNDKATK 300

Query: 301 ERTITTAYNALFLGDSDRLLQIMRKSFPELGLTPKDCIETNWIKSVLYIAGEPPKTPPEV 360
           ERTITTAYNALFLGDS+RLLQIMRKSFPELGLTPKDCIET+WIKSVLYIAGEPPKTPPEV
Sbjct: 301 ERTITTAYNALFLGDSNRLLQIMRKSFPELGLTPKDCIETSWIKSVLYIAGEPPKTPPEV 360

Query: 361 LLQGKPQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISENEI 420
           LLQGKPQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISENEI
Sbjct: 361 LLQGKPQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISENEI 420

Query: 421 PFPHRKGNLFKIQYVNPWQDGDKHETEHIEWIRELYNYMAPYVSKSPRAAYVNYRDLDLG 480
           PFPHRKGNLFKIQYVNPWQ GDKHET HIEWIRELYNYMAPYVSKSPRAAYVNYRDLDLG
Sbjct: 421 PFPHRKGNLFKIQYVNPWQAGDKHETRHIEWIRELYNYMAPYVSKSPRAAYVNYRDLDLG 480

Query: 481 MN-KDNTSLAHATEWGNRYFKNNFNRLVKVKTKVDPGNFFRHEQSIPPLAGSASFRTKKS 540
           MN KDNT+LA A EWGN+YFKNNFNRLVKVKTKVDPGNFFRHEQSIPPLA +ASFRTKKS
Sbjct: 481 MNKKDNTTLAQANEWGNKYFKNNFNRLVKVKTKVDPGNFFRHEQSIPPLASAASFRTKKS 540

Query: 541 RKVP 544
           ++ P
Sbjct: 541 KRSP 541

[Close]

BLAST of CSPI01G24800 vs. NCBI nr
Match: XP_038893903.1 (berberine bridge enzyme-like 13 [Benincasa hispida])

HSP 1 Score: 985.3 bits (2546), Expect = 2.1e-283
Identity = 486/530 (91.70%), Postives = 506/530 (95.47%), Query Frame = 0


Query: 1   MLYSGSSILILLLPFLLSFSVLSVSASLEQSFLQCVSLNSQQSVPPLSVCSQNNPSFFPL 60
           M +SGSSI ILLL FLLS S LS SASLEQSFLQCVS++SQQSVPP SVC+QNN SFFPL
Sbjct: 1   MFHSGSSIFILLLAFLLSLS-LSSSASLEQSFLQCVSVSSQQSVPPFSVCAQNNASFFPL 60

Query: 61  LQSTAQNLRFLDPSVPKPQFIFTPLHDTHVQAAVICSKKLNIHLRVRSGGHDYEGLSYAS 120
           LQ+TAQNLR+LDPSVPKP+FIFTPL+DTHVQAAVICSKKL IHLRVRSGGHDYEGLSYAS
Sbjct: 61  LQATAQNLRYLDPSVPKPEFIFTPLYDTHVQAAVICSKKLRIHLRVRSGGHDYEGLSYAS 120

Query: 121 ETETPFIVIDLAKLRSVEVNIQDNTAWAQAGATVGEVYYRISQKSNVHGFPAGLCTSLGI 180
           E ETPFIV+DLAKLRSVEVNIQDNTAW QAGATVGEVYYRIS+KSNVHGFPAGLCTSLGI
Sbjct: 121 EIETPFIVVDLAKLRSVEVNIQDNTAWVQAGATVGEVYYRISEKSNVHGFPAGLCTSLGI 180

Query: 181 GGHITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDRSTMGEDYFWAIRGGAGGSFGII 240
           GGHITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDRS MGEDYFWAIRGGAGGSFGII
Sbjct: 181 GGHITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDRSGMGEDYFWAIRGGAGGSFGII 240

Query: 241 LWWKLQLVPVPETVTVFTVPKTLEQGATQILYKWQQVADKLDEDLFIRVIIQVTNDKATK 300
           LWWKLQLVPVPETVTVFTVPKTLEQGATQILYKWQQVADKLDEDLFIRV+IQVTNDKATK
Sbjct: 241 LWWKLQLVPVPETVTVFTVPKTLEQGATQILYKWQQVADKLDEDLFIRVLIQVTNDKATK 300

Query: 301 ERTITTAYNALFLGDSDRLLQIMRKSFPELGLTPKDCIETNWIKSVLYIAGEPPKTPPEV 360
           ERTITTAYNALFLGDS+RLLQIM KSFPELGLTPKDCIET+WIKSVLYIAGE PKTPPEV
Sbjct: 301 ERTITTAYNALFLGDSNRLLQIMGKSFPELGLTPKDCIETSWIKSVLYIAGEAPKTPPEV 360

Query: 361 LLQGKPQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISENEI 420
           LLQGKPQF+NYFKAKSDFVQVPIPETGLEGLWK  LQE+ PLMIWNPYGGMMSKISENEI
Sbjct: 361 LLQGKPQFRNYFKAKSDFVQVPIPETGLEGLWKRLLQEDHPLMIWNPYGGMMSKISENEI 420

Query: 421 PFPHRKGNLFKIQYVNPWQDGDKHETEHIEWIRELYNYMAPYVSKSPRAAYVNYRDLDLG 480
           PFPHRKGNLFKIQYVN WQDG K E +H+EWIRELYNYMAPYVSKSPRAAYVNYRDLDLG
Sbjct: 421 PFPHRKGNLFKIQYVNSWQDGHKDEEKHMEWIRELYNYMAPYVSKSPRAAYVNYRDLDLG 480

Query: 481 MN-KDNTSLAHATEWGNRYFKNNFNRLVKVKTKVDPGNFFRHEQSIPPLA 530
           MN K NTSLA A+EWGN+YFK+NFNRLVKVKTKVDPGNFFRHEQSIPPLA
Sbjct: 481 MNKKGNTSLAQASEWGNKYFKDNFNRLVKVKTKVDPGNFFRHEQSIPPLA 529

[Close]

BLAST of CSPI01G24800 vs. NCBI nr
Match: XP_022140062.1 (berberine bridge enzyme-like 13 [Momordica charantia])

HSP 1 Score: 932.6 bits (2409), Expect = 1.6e-267
Identity = 462/540 (85.56%), Postives = 491/540 (90.93%), Query Frame = 0


Query: 4   SGSSILILLLPFLLSFSVLSVSASLEQSFLQCVSLNSQQSVPPLSVCSQNNPSFFPLLQS 63
           SGS I  L+L FLLSFS LS SA LEQ+F QC+S+NSQ S PP SVC  NN SF  LLQS
Sbjct: 5   SGSPIFHLVLAFLLSFS-LSNSAPLEQAFFQCLSVNSQLSAPPFSVCDPNNASFTTLLQS 64

Query: 64  TAQNLRFLDPSVPKPQFIFTPLHDTHVQAAVICSKKLNIHLRVRSGGHDYEGLSYASETE 123
           TAQNLR+LD SVPKP+FIF PL D+HVQAAVICSKKL IHLRVRSGGHDYEGLSYASE E
Sbjct: 65  TAQNLRYLDASVPKPEFIFIPLLDSHVQAAVICSKKLRIHLRVRSGGHDYEGLSYASEIE 124

Query: 124 TPFIVIDLAKLRSVEVNIQDNTAWAQAGATVGEVYYRISQKSNVHGFPAGLCTSLGIGGH 183
           TPFIV+DL KLRSVEVNI+DN+AW QAGATVGEVYYRIS+KS VHGFPAGLCTSLGIGGH
Sbjct: 125 TPFIVVDLTKLRSVEVNIEDNSAWVQAGATVGEVYYRISEKSEVHGFPAGLCTSLGIGGH 184

Query: 184 ITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDRSTMGEDYFWAIRGGAGGSFGIILWW 243
           ITGGAYGSMMRKYGLGADNVIDARIVD+NGRILDR+ MGED+FWAIRGGAGGSFGIILWW
Sbjct: 185 ITGGAYGSMMRKYGLGADNVIDARIVDINGRILDRAAMGEDHFWAIRGGAGGSFGIILWW 244

Query: 244 KLQLVPVPETVTVFTVPKTLEQGATQILYKWQQVADKLDEDLFIRVIIQVTNDKATKERT 303
           KLQLVPVPETVTVFTVPKTLEQGATQ+LYKWQQVA KLDE+LFIRVIIQ T+DKATK+RT
Sbjct: 245 KLQLVPVPETVTVFTVPKTLEQGATQVLYKWQQVAHKLDENLFIRVIIQPTSDKATKKRT 304

Query: 304 ITTAYNALFLGDSDRLLQIMRKSFPELGLTPKDCIETNWIKSVLYIAGEPPKTPPEVLLQ 363
           ITTAYNALFLGDS+RLLQ+M KSFPELGLTPKDCIET+WIKSVLYIAGEPP TPPEVLLQ
Sbjct: 305 ITTAYNALFLGDSNRLLQVMGKSFPELGLTPKDCIETSWIKSVLYIAGEPPGTPPEVLLQ 364

Query: 364 GKPQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISENEIPFP 423
           GKPQFKNYFKAKSDFVQVPIPE GLEGLWK  L E+ PL IWNPYGGMMSKISENEIPFP
Sbjct: 365 GKPQFKNYFKAKSDFVQVPIPEAGLEGLWKRLLVEDKPLTIWNPYGGMMSKISENEIPFP 424

Query: 424 HRKGNLFKIQYVNPWQDGDKHETEHIEWIRELYNYMAPYVSKSPRAAYVNYRDLDLGMN- 483
           HRKGNLFKIQY++ WQ GD    +HIEWIRELYNYMAPYVS SPRAAYVNYRDLDLGMN 
Sbjct: 425 HRKGNLFKIQYLSTWQGGDTDAAKHIEWIRELYNYMAPYVSTSPRAAYVNYRDLDLGMNK 484

Query: 484 KDNTSLAHATEWGNRYFKNNFNRLVKVKTKVDPGNFFRHEQSIPPLAGSASF-RTKKSRK 542
           KDNTSL  ATEWGN+YFK+NFNRLVKVKTKVDP NFFRHEQSIPPLAG+ASF RT K R+
Sbjct: 485 KDNTSLVQATEWGNKYFKDNFNRLVKVKTKVDPENFFRHEQSIPPLAGTASFKRTTKYRR 543

[Close]

BLAST of CSPI01G24800 vs. NCBI nr
Match: XP_022994774.1 (berberine bridge enzyme-like 13 [Cucurbita maxima])

HSP 1 Score: 924.9 bits (2389), Expect = 3.3e-265
Identity = 460/546 (84.25%), Postives = 494/546 (90.48%), Query Frame = 0


Query: 1   MLYSGSSILILLLP---FLLSFSVLSVSASLEQSFLQCVSLNSQQSVPPLSVCSQNNPSF 60
           ML S SSIL LLL    FLLS SV S SA LEQSFL C+S NSQQSVPP SVC+  N SF
Sbjct: 1   MLNSTSSILPLLLTLLVFLLSVSV-SNSALLEQSFLHCLSTNSQQSVPPFSVCAPTNASF 60

Query: 61  FPLLQSTAQNLRFLDPSVPKPQFIFTPLHDTHVQAAVICSKKLNIHLRVRSGGHDYEGLS 120
            PLLQSTAQNLR+ DPSVPKP+FIFTPL+DTHVQAAV+CSK L IHLRVRSGGHDYEGLS
Sbjct: 61  IPLLQSTAQNLRYSDPSVPKPEFIFTPLYDTHVQAAVVCSKGLQIHLRVRSGGHDYEGLS 120

Query: 121 YASETETPFIVIDLAKLRSVEVNIQDNTAWAQAGATVGEVYYRISQKSNVHGFPAGLCTS 180
           Y S+ ETPFIV+DLAKLRSVEVNI+DNTAW QAGATVGEVYYRIS+KSNVHGFPAGLCTS
Sbjct: 121 YVSQIETPFIVVDLAKLRSVEVNIEDNTAWVQAGATVGEVYYRISEKSNVHGFPAGLCTS 180

Query: 181 LGIGGHITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDRSTMGEDYFWAIRGGAGGSF 240
           LGIGGHITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDR  MGED+FWAIRGGAGGSF
Sbjct: 181 LGIGGHITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDRKAMGEDHFWAIRGGAGGSF 240

Query: 241 GIILWWKLQLVPVPETVTVFTVPKTLEQGATQILYKWQQVADKLDEDLFIRVIIQVTNDK 300
           GIILWWKL+LVPVPETVTVFTVP+TLEQGATQILYKWQQ+ADKLDEDLFIRVIIQV++DK
Sbjct: 241 GIILWWKLRLVPVPETVTVFTVPRTLEQGATQILYKWQQIADKLDEDLFIRVIIQVSSDK 300

Query: 301 ATKERTITTAYNALFLGDSDRLLQIMRKSFPELGLTPKDCIETNWIKSVLYIAGEPPKTP 360
            TK+RTITTAYNALFLGDS+RLLQ+M KSFPELGLT KDC ET+WIKSVLYIAGE P+TP
Sbjct: 301 TTKQRTITTAYNALFLGDSNRLLQVMGKSFPELGLTSKDCTETSWIKSVLYIAGEAPRTP 360

Query: 361 PEVLLQGKPQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISE 420
           PEVLLQGKPQFK+YFKAKSDFV VPIPE GLEGLWK  L ++ P MIWNPYGGMMSKISE
Sbjct: 361 PEVLLQGKPQFKSYFKAKSDFVHVPIPEVGLEGLWKKLLLDDMPFMIWNPYGGMMSKISE 420

Query: 421 NEIPFPHRKGNLFKIQYVNPWQDGDKHETEHIEWIRELYNYMAPYVSKSPRAAYVNYRDL 480
           NEIPFPHRKGNLFKIQY+  WQ G+K   +HIEWIRELYNYMAPYVSKSPRAAYVNYRDL
Sbjct: 421 NEIPFPHRKGNLFKIQYLTSWQGGNKDVEKHIEWIRELYNYMAPYVSKSPRAAYVNYRDL 480

Query: 481 DLGMN-KDNTSLAHATEWGNRYFKNNFNRLVKVKTKVDPGNFFRHEQSIPPLAGSASFR- 540
           DLGMN K+NTSLA A+EWGN+YFK+NFNRL+KVKTKVDP NFFRHEQSIPP A + SFR 
Sbjct: 481 DLGMNKKNNTSLAQASEWGNKYFKDNFNRLIKVKTKVDPDNFFRHEQSIPPFAVTKSFRK 540

Query: 541 TKKSRK 542
           T+K RK
Sbjct: 541 TRKFRK 545

[Close]

BLAST of CSPI01G24800 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 711.8 bits (1836), Expect = 4.1e-205
Identity = 346/530 (65.28%), Postives = 423/530 (79.81%), Query Frame = 0


Query: 1   MLYSGSSILILLLPFLLSFSVLSVSASLEQSFLQCVSLNSQQSVPPLS---VCSQNNPSF 60
           ++ + ++ L+ LL   LS+  LS S +++Q F+ C+  NS  S P  S       N  SF
Sbjct: 5   LMNNTNAFLVTLLLLSLSYIPLSFS-TIQQDFVMCLVDNSDASFPMDSSFFTHDLNASSF 64

Query: 61  FPLLQSTAQNLRFLDPSVPKPQFIFTPLHDTHVQAAVICSKKLNIHLRVRSGGHDYEGLS 120
              L+++AQNLR+L PS PKP+FIF PL++THVQAAV+C+KKL +HLR+RSGGHDYEGLS
Sbjct: 65  KLALETSAQNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLS 124

Query: 121 YASETETPFIVIDLAKLRSVEVNIQDNTAWAQAGATVGEVYYRISQKSNVHGFPAGLCTS 180
           Y SE ET F+++DL+KLR + V+I+ N+AW  AGA++GEVYYRI +KS +HGFPAGLCTS
Sbjct: 125 YVSEMETAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTS 184

Query: 181 LGIGGHITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDRSTMGEDYFWAIRGGAGGSF 240
           LGIGGHI GGAYGSMMRK+GLGADNV+DARIVD +G+IL+R+ MGED FWAIRGG GGSF
Sbjct: 185 LGIGGHIIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSF 244

Query: 241 GIILWWKLQLVPVPETVTVFTVPKTLEQGATQILYKWQQVADKLDEDLFIRVIIQVTN-D 300
           G+IL WK++LVPVPE VTVFTV +TLEQ  T++LYKWQQVADKLDEDLFIRVIIQ T+  
Sbjct: 245 GVILAWKIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKT 304

Query: 301 KATKERTITTAYNALFLGDSDRLLQIMRKSFPELGLTPKDCIETNWIKSVLYIAGEPPKT 360
             +KERTI+T+Y   FLGD++RLLQ+M++SFP+LGLT KDC+ET+WIKSV+YIAG P   
Sbjct: 305 PKSKERTISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTA 364

Query: 361 PPEVLLQGKPQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKIS 420
           P E LL GK  FKNYFKAKSD+V+ PIP  GLEGLW+  L+E+SPL IWNPYGGMM+KI 
Sbjct: 365 PSEALLDGKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWNPYGGMMAKIP 424

Query: 421 ENEIPFPHRKGNLFKIQYVNPWQDGDKHETEHIEWIRELYNYMAPYVSKSPRAAYVNYRD 480
           E E PFPHR G LFKIQ++  WQDG   E +H+ W+RE+Y+YM  YVSKSPR+AYVNYRD
Sbjct: 425 ETETPFPHRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRD 484

Query: 481 LDLGMNKDNTSLAHATEWGNRYFKNNFNRLVKVKTKVDPGNFFRHEQSIP 527
           LDLGMN   +    A EWGNRYFK NF RLV++K K DP NFFRHEQSIP
Sbjct: 485 LDLGMNGKGSD---AREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIP 530

[Close]

BLAST of CSPI01G24800 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 711.8 bits (1836), Expect = 4.1e-205
Identity = 347/525 (66.10%), Postives = 417/525 (79.43%), Query Frame = 0


Query: 11  LLLPFLLSFSVLSVSASLEQSFLQCVSLNSQQSVPPLSVC---SQNNPSFFPLLQSTAQN 70
           L L  LL  SV   S++L+Q F++C+  NS  S P  +      QN   F   L+STAQN
Sbjct: 12  LFLVTLLLISVPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTAQN 71

Query: 71  LRFLDPSVPKPQFIFTPLHDTHVQAAVICSKKLNIHLRVRSGGHDYEGLSYASETETPFI 130
           LR+L PS PKP FIF PL++THVQAAV+C+KKL +HLR+RSGGHDYEGLS+ +E ETPF+
Sbjct: 72  LRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFV 131

Query: 131 VIDLAKLRSVEVNIQDNTAWAQAGATVGEVYYRISQKSNVHGFPAGLCTSLGIGGHITGG 190
           ++DL+KLR V+V++  N+AWA AGAT+GEVYYRI +KS  HGFPAGLC+SLGIGGH+ GG
Sbjct: 132 IVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGG 191

Query: 191 AYGSMMRKYGLGADNVIDARIVDVNGRILDRSTMGEDYFWAIRGGAGGSFGIILWWKLQL 250
           AYGSMMRK+GLGADNV+DARIVD NG+ILDR+ MGED FWAIRGG GGSFG+IL WK++L
Sbjct: 192 AYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKL 251

Query: 251 VPVPETVTVFTVPKTLEQGATQILYKWQQVADKLDEDLFIRVIIQVTNDKATK--ERTIT 310
           VPVP TVTVFTV KTLEQ  T++LYKW+Q+ADKLD+DLFIRVII   + K TK   RTI+
Sbjct: 252 VPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPAS-KTTKPGNRTIS 311

Query: 311 TAYNALFLGDSDRLLQIMRKSFPELGLTPKDCIETNWIKSVLYIAGEPPKTPPEVLLQGK 370
            +Y A FLGDS+RLLQ+M+KSFPELGLT KDC E +WIKSV+YIAG P    PE LL GK
Sbjct: 312 MSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALLAGK 371

Query: 371 PQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISENEIPFPHR 430
             FKN+FKAKSDFV+ PIP  GLEGLW+ FL+E+SPL IWNPYGGMMS+ISE+EIPFPHR
Sbjct: 372 SLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGGMMSRISESEIPFPHR 431

Query: 431 KGNLFKIQYVNPWQDGDKHETEHIEWIRELYNYMAPYVSKSPRAAYVNYRDLDLGMNKDN 490
            G LFKIQ+++ WQDG   E  H++WIRE+Y+YM  YVSK+PR AYVNYRDLDLG N+  
Sbjct: 432 NGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNEGE 491

Query: 491 TSLAHATEWGNRYFKNNFNRLVKVKTKVDPGNFFRHEQSIPPLAG 531
           T    A EWG +Y+K NF RLVK+K + DP NFFRHEQS+P   G
Sbjct: 492 TD---AREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPTKIG 532

[Close]

BLAST of CSPI01G24800 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 692.2 bits (1785), Expect = 3.4e-199
Identity = 342/529 (64.65%), Postives = 416/529 (78.64%), Query Frame = 0


Query: 7   SILILLLPFLLSFSVLSVSASLEQSFLQCVSLNSQQSVP---PLSVCSQNNPSFFPLLQS 66
           SIL+L    L + +  S  ASL+  F+ CV  N+    P        ++N   F  +L+S
Sbjct: 11  SILVLYFS-LYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLES 70

Query: 67  TAQNLRFLDPSVPKPQFIFTPLHDTHVQAAVICSKKLNIHLRVRSGGHDYEGLSYASETE 126
           TAQNLRFL  S+PKP FIF+P+H++HVQA++ICSKKL +HLRVRSGGHDYEGLSY S+ +
Sbjct: 71  TAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQID 130

Query: 127 TPFIVIDLAKLRSVEVNIQDNTAWAQAGATVGEVYYRISQKSNVHGFPAGLCTSLGIGGH 186
            PFI++DL+K+R V +NIQDN+AW Q+GATVGE+YYRI++KS VHGFPAGLC+SLGIGGH
Sbjct: 131 KPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGH 190

Query: 187 ITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDRSTMGEDYFWAIRGGAGGSFGIILWW 246
           ITGGAYGSMMRKYGLGADNV+DA+IVD NG++LDR+ MGED FWAIRGGAGGSFGIIL W
Sbjct: 191 ITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAW 250

Query: 247 KLQLVPVPETVTVFTVPKTLEQG-ATQILYKWQQVADKLDEDLFIRVIIQVTNDKATKER 306
           K++LVPVP+TVTVFTV KTL+Q    +I+ KWQ+VADKL E+LFIRV+  V      K  
Sbjct: 251 KIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTGGNK-- 310

Query: 307 TITTAYNALFLGDSDRLLQIMRKSFPELGLTPKDCIETNWIKSVLYIAGEPPKTPPEVLL 366
           T+TT+YNALFLG    L+ +M+KSFPELGLT KDCIE +W++S+ YI+G P  TP  VLL
Sbjct: 311 TVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLL 370

Query: 367 QGKPQF-KNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISENEIP 426
           QGK  F K  FKAKSDFV+ PIPE+GL+G++K  L+E+ PLMIWNPYGGMM+KI E++IP
Sbjct: 371 QGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPLMIWNPYGGMMAKIPESQIP 430

Query: 427 FPHRKGNLFKIQYVNPWQDGDKHETEHIEWIRELYNYMAPYVSKSPRAAYVNYRDLDLGM 486
           FPHRKG LFK+QYV  W D DK  + HI WIR+LY+YM PYVS +PR AYVNYRDLDLG 
Sbjct: 431 FPHRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGR 490

Query: 487 N-KD-NTSLAHATEWGNRYFKNNFNRLVKVKTKVDPGNFFRHEQSIPPL 529
           N KD  T +  A  WG  YFKNNFNRL+ +K KVDP NFFRHEQSIPP+
Sbjct: 491 NTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536

[Close]

BLAST of CSPI01G24800 vs. TAIR 10
Match: AT5G44390.1 (FAD-binding Berberine family protein )

HSP 1 Score: 636.3 bits (1640), Expect = 2.2e-182
Identity = 315/532 (59.21%), Postives = 397/532 (74.62%), Query Frame = 0


Query: 6   SSILILLLPFLLSFSVLSVSASLEQSFLQCV--SLNSQQSVPPLSVCSQNNPSFF-PLLQ 65
           S I +  L F      L+ S SL+  F++C+  + N + ++       + N S F  +L+
Sbjct: 11  SCISVFALYFSFYTITLTSSTSLQDDFIKCLYRNTNVRFTLDKTFFTPERNASIFTEVLE 70

Query: 66  STAQNLRFLDPSVPKPQFIFTPLHDTHVQAAVICSKKLNIHLRVRSGGHDYEGLSYASET 125
           STAQN R+L  ++PKP FIF P+H++HVQA+VICSKKL IH RVRSGGHDYEG+SY S+ 
Sbjct: 71  STAQNQRYLTKTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYEGVSYVSQI 130

Query: 126 ETPFIVIDLAKLRSVEVNIQDNTAWAQAGATVGEVYYRISQKSNVHGFPAGLCTSLGIGG 185
           E PF++IDL+KLR + V+I+D +AW +AGATVGE+YYRI++KS  HGFPAG+  SLGIGG
Sbjct: 131 EKPFVLIDLSKLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPAGVYPSLGIGG 190

Query: 186 HITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDRSTMGEDYFWAIRGGAGGSFGIILW 245
           HITGGAYGS+MRKYGL ADNV+DA+IVD NG++LDR++MGED FWAIRGG+GGSFGIIL 
Sbjct: 191 HITGGAYGSLMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGIILS 250

Query: 246 WKLQLVPVPETVTVFTVPKTLEQGAT-QILYKWQQVADKLDEDLFIRVIIQVTNDKATKE 305
           WK++LVPVPET+TVFTV KT EQ  + +IL KWQ++AD L ++LF+RV   V+ +KA K 
Sbjct: 251 WKIKLVPVPETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFTVSGNKANK- 310

Query: 306 RTITTAYNALFLGDSDRLLQIMRKSFPELGLTPKDCIETNWIKSVLYIAGEPPKTPP--E 365
            T+T AY   FLG+   L+++M+K FPELGLT KDCIE +WI S++Y +G P   PP  E
Sbjct: 311 -TVTMAYIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFPTNPPPPIE 370

Query: 366 VLLQGK-PQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISEN 425
           +LLQ K P  K YFK KSDF + PIP  GLEG++K  L+E++ L+IW PYGG M KI E+
Sbjct: 371 ILLQAKSPIGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEEDAALVIWTPYGGKMDKIPES 430

Query: 426 EIPFPHRKGNLFKIQYVNPWQDGDKHETEHIEWIRELYNYMAPYVSKSPRAAYVNYRDLD 485
           EIPFPHR G  F IQY   W D +K      +WIRELY YM PYVS +PR AYVNYRDLD
Sbjct: 431 EIPFPHRNGTNFMIQYYRSWSDSEKRPNRRTKWIRELYGYMTPYVSSNPRQAYVNYRDLD 490

Query: 486 LGMNKDN--TSLAHATEWGNRYFKNNFNRLVKVKTKVDPGNFFRHEQSIPPL 529
           LG NKDN  ++   A  WG  YFK+NFNRLV++K+KVDP NFFRHEQSIP L
Sbjct: 491 LGQNKDNSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPTL 540

[Close]

BLAST of CSPI01G24800 vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 612.5 bits (1578), Expect = 3.4e-175
Identity = 299/531 (56.31%), Postives = 389/531 (73.26%), Query Frame = 0


Query: 6   SSILILLLPF-LLSFSVLSVSASLEQSFLQCVSLNSQQSVP---PLSVCSQNNPSFFPLL 65
           S I++ +L F     +  S SAS++  F+ CV  N+  S P    L   ++N   F  +L
Sbjct: 11  SCIIVSVLYFSFYCITPTSSSASIQDQFINCVKRNTHVSFPLEKTLFTPAKNVSLFNQVL 70

Query: 66  QSTAQNLRFLDPSVPKPQFIFTPLHDTHVQAAVICSKKLNIHLRVRSGGHDYEGLSYASE 125
           +STAQNL+FL  S+PKP FIF P+H + VQA++ICSKKL IH RVRSGGHD+E LSY S 
Sbjct: 71  ESTAQNLQFLAKSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYVSR 130

Query: 126 TETPFIVIDLAKLRSVEVNIQDNTAWAQAGATVGEVYYRISQKSNVHGFPAGLCTSLGIG 185
            E PFI++DL+KL+ + V+I+ N+AW Q GAT+GE+YYRI++KS +HGFPAGLCTS+GIG
Sbjct: 131 IEKPFILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIG 190

Query: 186 GHITGGAYGSMMRKYGLGADNVIDARIVDVNGRILDRSTMGEDYFWAIRGGAGGSFGIIL 245
           G++TGG YG++MRKYGL  DNV+D ++VD NG++LDR+ MGED FWAIRGG G SFGI+L
Sbjct: 191 GYMTGGGYGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVL 250

Query: 246 WWKLQLVPVPETVTVFTVPKTLEQGA-TQILYKWQQVADKLDEDLFIRVIIQVTNDKATK 305
            WK++LVPVP+TVTVFTV KTLEQ A  + + KWQQ++ K+ E++ IRV+++   +   K
Sbjct: 251 AWKIKLVPVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLRAAGNDGNK 310

Query: 306 ERTITTAYNALFLGDSDRLLQIMRKSFPELGLTPKDCIETNWIKSVLYIAGEPPKTPPEV 365
             T+T  Y   FLG+   LL++M K+FPELGLT KDC E +WI++ L+  G P  +P E+
Sbjct: 311 --TVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGGFPTGSPIEI 370

Query: 366 LLQGK-PQFKNYFKAKSDFVQVPIPETGLEGLWKIFLQEESPLMIWNPYGGMMSKISENE 425
           LLQ K P  K+YFKA SDFV+ PIP  GL+G++K  ++  +  + W PYGGMMSKI E+ 
Sbjct: 371 LLQLKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRLIEGNTTFLNWTPYGGMMSKIPESA 430

Query: 426 IPFPHRKGNLFKIQYVNPWQDGDKHETEHIEWIRELYNYMAPYVSKSPRAAYVNYRDLDL 485
           IPFPHR G LFKI Y   W + DK  +  I WI+E+YNYMAPYVS +PR AYVNYRDLD 
Sbjct: 431 IPFPHRNGTLFKILYYANWLENDKTSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRDLDF 490

Query: 486 GMNKDN--TSLAHATEWGNRYFKNNFNRLVKVKTKVDPGNFFRHEQSIPPL 529
           G NK+N   +   A  WG +YFK NF+RLVK+KTKVDP NFFRHEQSIPP+
Sbjct: 491 GQNKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPM 539

The following BLAST results are available for this feature:

BLAST of CSPI01G24800 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93ZA3	5.8e-204	65.28	Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=...	[more]
O64743	5.8e-204	66.10	Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV...	[more]
Q9FKU8	4.8e-198	64.65	Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=...	[more]
Q9FKU9	3.1e-181	59.21	Berberine bridge enzyme-like 25 OS=Arabidopsis thaliana OX=3702 GN=At5g44390 PE=...	[more]
Q9FKV0	4.8e-174	56.31	Berberine bridge enzyme-like 24 OS=Arabidopsis thaliana OX=3702 GN=At5g44380 PE=...	[more]

BLAST of CSPI01G24800 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7V956	6.3e-302	94.85	Flavin-dependent oxidoreductase FOX5-like OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3BDU6	6.3e-302	94.85	flavin-dependent oxidoreductase FOX5-like OS=Cucumis melo OX=3656 GN=LOC10348858...	[more]
A0A6J1CFR3	7.7e-268	85.56	berberine bridge enzyme-like 13 OS=Momordica charantia OX=3673 GN=LOC111010807 P...	[more]
A0A6J1K3T7	1.6e-265	84.25	berberine bridge enzyme-like 13 OS=Cucurbita maxima OX=3661 GN=LOC111490401 PE=3...	[more]
A0A6J1EJU9	1.8e-264	83.95	berberine bridge enzyme-like 13 OS=Cucurbita moschata OX=3662 GN=LOC111433979 PE...	[more]

BLAST of CSPI01G24800 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004135540.1	0.0e+00	99.82	berberine bridge enzyme-like 15 [Cucumis sativus] >KGN66361.2 hypothetical prote...	[more]
XP_008445619.2	1.3e-301	94.85	PREDICTED: flavin-dependent oxidoreductase FOX5-like [Cucumis melo] >KAA0063744....	[more]
XP_038893903.1	2.1e-283	91.70	berberine bridge enzyme-like 13 [Benincasa hispida]	[more]
XP_022140062.1	1.6e-267	85.56	berberine bridge enzyme-like 13 [Momordica charantia]	[more]
XP_022994774.1	3.3e-265	84.25	berberine bridge enzyme-like 13 [Cucurbita maxima]	[more]

BLAST of CSPI01G24800 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G30760.1	4.1e-205	65.28	FAD-binding Berberine family protein	[more]
AT2G34790.1	4.1e-205	66.10	FAD-binding Berberine family protein	[more]
AT5G44400.1	3.4e-199	64.65	FAD-binding Berberine family protein	[more]
AT5G44390.1	2.2e-182	59.21	FAD-binding Berberine family protein	[more]
AT5G44380.1	3.4e-175	56.31	FAD-binding Berberine family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016169	FAD-binding, type PCMH, subdomain 2	GENE3D	3.30.465.10		coord: 139..527 e-value: 1.1E-170 score: 570.2
IPR012951	Berberine/berberine-like	PFAM	PF08031	BBE	coord: 470..526 e-value: 2.3E-23 score: 82.0
IPR016167	FAD-binding, type PCMH, subdomain 1	GENE3D	3.30.43.10		coord: 21..134 e-value: 1.6E-43 score: 148.9
IPR006094	FAD linked oxidase, N-terminal	PFAM	PF01565	FAD_binding_4	coord: 78..217 e-value: 5.3E-27 score: 94.3
None	No IPR available	GENE3D	3.40.462.20		coord: 253..466 e-value: 1.1E-170 score: 570.2
None	No IPR available	PANTHER	PTHR32448	OS08G0158400 PROTEIN	coord: 7..529
None	No IPR available	PANTHER	PTHR32448:SF35	BERBERINE BRIDGE ENZYME-LIKE 26	coord: 7..529
IPR016166	FAD-binding domain, PCMH-type	PROSITE	PS51387	FAD_PCMH	coord: 74..250 score: 17.218624
IPR036318	FAD-binding, type PCMH-like superfamily	SUPERFAMILY	56176	FAD-binding/transporter-associated domain-like	coord: 73..251

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G24800.1	CSPI01G24800.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0071949	FAD binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0050660	flavin adenine dinucleotide binding