CSPI01G24430 (gene) Cucumber (PI 183967) v1

BLAST of CSPI01G24430 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 1.1e-71
Identity = 194/536 (36.19%), Postives = 292/536 (54.48%), Query Frame = 0

Query: 10  QWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 69
           +W  L+  IW+Q+  GTNF+F +YSS LK  L +SQ++LN LA ASD GK F   SG+A 
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 70  NYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWINTVCYMV 129
            Y PL +VLF  +++G +GYGVQ+L ITN    P Y L+F   +LAG SICW NT C+++
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFNTACFIL 162

Query: 130 AITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLLNSVLPLGVC 189
            I +F  ++R +A+ ++ S+ G+SA +Y     S+  N  +  ++  +LLLNS++PL V 
Sbjct: 163 CIRHF-PNNRALALSLTVSFNGISAALY-----SLAFNAINPSSSNLYLLLNSLVPLVVS 222

Query: 190 ------VLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSALGG 249
                 VL  P        + +      F ++ V+ + T     ++S  + S  L+ +G 
Sbjct: 223 FAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIGA 282

Query: 250 LVGIMVFLVLPLVVVV----VEKVKERVEEGKEG---------KVYHFTVEEKNDEERMR 309
           +V ++  L  PL+V      +  +  R+     G         K    +V  K   E M 
Sbjct: 283 VVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMG 342

Query: 310 GENERKVERTDDGEAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGS 369
              E    R  D     E   + ++ R+ FWLY   Y  G T+GL + NNLGQIA+S G 
Sbjct: 343 TAKEGNTVRLGD-----EHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQ 402

Query: 370 SSVSSLVSLSSSFGFFGRLLPSILDYFLSRRNKFMKSKPGWM-VGLMGTLCGGFFLLLSP 429
           +S ++LV++ SSF FFGRLL +  D+      +F  ++ GW  + L+ T    F L +S 
Sbjct: 403 NS-TTLVTIYSSFSFFGRLLSAAPDFM---HKRFRLTRTGWFAIALLPTPIAFFLLAVSS 462

Query: 430 S-DTSLCMSTAIIAICTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAA 489
           S  T+L  +TA+I + +G I + +VS T+DLFG  +  +NHNI++ NIP GS ++GY+AA
Sbjct: 463 SQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGYIAA 522

Query: 490 FLYHKQAGHGVDP-----GKCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFY 520
            +Y   A   + P       CIG +CY  TF+ WG  S  G   +L L+ RTK  Y
Sbjct: 523 SIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of CSPI01G24430 vs. ExPASy TrEMBL
Match: A0A0A0LYD4 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G533690 PE=4 SV=1)

HSP 1 Score: 1003.4 bits (2593), Expect = 3.5e-289
Identity = 530/531 (99.81%), Postives = 530/531 (99.81%), Query Frame = 0

Query: 1   MPSSSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKL 60
           MPSSSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKL
Sbjct: 1   MPSSSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKL 60

Query: 61  FACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSIC 120
           FACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSIC
Sbjct: 61  FACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSIC 120

Query: 121 WINTVCYMVAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLL 180
           WINTVCYMVAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLL
Sbjct: 121 WINTVCYMVAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLL 180

Query: 181 NSVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSA 240
           NSVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSA
Sbjct: 181 NSVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSA 240

Query: 241 LGGLVGIMVFLVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEKNDEERMRGENERKVERT 300
           LGGLVGIMVFLVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEKNDEERMRGENERKVERT
Sbjct: 241 LGGLVGIMVFLVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEKNDEERMRGENERKVERT 300

Query: 301 DDGEAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360
           DDGEAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS
Sbjct: 301 DDGEAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360

Query: 361 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGTLCGGFFLLLSPSDTSLCMSTAI 420
           SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGTLCGGFFLLLSPSDTSLCMSTAI
Sbjct: 361 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGTLCGGFFLLLSPSDTSLCMSTAI 420

Query: 421 IAICTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLYHKQAGHGVD 480
           IAICTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLY KQAGHGVD
Sbjct: 421 IAICTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLYRKQAGHGVD 480

Query: 481 PGKCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYSTTKKSSYIPSN 532
           PGKCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYSTTKKSSYIPSN
Sbjct: 481 PGKCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYSTTKKSSYIPSN 531

BLAST of CSPI01G24430 vs. ExPASy TrEMBL
Match: A0A1S3C569 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103497028 PE=4 SV=1)

HSP 1 Score: 948.3 bits (2450), Expect = 1.3e-272
Identity = 505/529 (95.46%), Postives = 512/529 (96.79%), Query Frame = 0

Query: 4   SSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 63
           SSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC
Sbjct: 3   SSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 62

Query: 64  FSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWIN 123
           FSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWIN
Sbjct: 63  FSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWIN 122

Query: 124 TVCYMVAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLLNSV 183
           TVCYMVAITNF SS+RQVAVGISTSYQGLSAKVYTDIVGSIFSNK SSKTAETFLLLNSV
Sbjct: 123 TVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLNSV 182

Query: 184 LPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSALGG 243
           LPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATG FAT+TSVGSVSRMLSAL G
Sbjct: 183 LPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSALSG 242

Query: 244 LVGIMVFLVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEKNDEER-MRGENERKVERTDD 303
           LVGIMVFLVLPLVVVV EKVKERVEEGKEG+VYHFTVEE ND ER MRGENERKVERTD+
Sbjct: 243 LVGIMVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEENNDVERIMRGENERKVERTDE 302

Query: 304 GEAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSS 363
           GEAMEEIGAKEMVKRINFWLY GVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSS
Sbjct: 303 GEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSS 362

Query: 364 FGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGTLCGGFFLLLSPSDTSLCMSTAIIA 423
           FGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMG LCGGFFLLLSPSDTSLCMSTAIIA
Sbjct: 363 FGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTAIIA 422

Query: 424 ICTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLYHKQAGHGVDPG 483
           ICTGAITSISVSTTTDLFGATNFSINHN+VVANIPFGSFIFGYMAAFLYHKQAGHGV  G
Sbjct: 423 ICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVAAG 482

Query: 484 KCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYSTTKKSSYIPSN 532
           KCIGVECYRTTFLIWG FSSFGTFLALLLFART+SFYSTT  SS+I  N
Sbjct: 483 KCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLSSHISPN 531

BLAST of CSPI01G24430 vs. ExPASy TrEMBL
Match: A0A5D3BBQ0 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G00460 PE=4 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.5e-255
Identity = 481/529 (90.93%), Postives = 488/529 (92.25%), Query Frame = 0

Query: 4   SSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 63
           SSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC
Sbjct: 3   SSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 62

Query: 64  FSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWIN 123
           FSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWIN
Sbjct: 63  FSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWIN 122

Query: 124 TVCYMVAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLLNSV 183
           TVCYMVAITNF SS+RQVAVGISTSYQGLSAKVYTDIVGSIFSNK SSKTAETFLLLNSV
Sbjct: 123 TVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLNSV 182

Query: 184 LPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSALGG 243
           LPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATG FAT+TSVGSVSRMLSAL G
Sbjct: 183 LPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSALSG 242

Query: 244 LVGIMVFLVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEKNDEER-MRGENERKVERTDD 303
           LVGIMVFLVLPLVVVV EKVKERVEEGKEG+VYHFTVEE ND ER MRGENERKVERTD+
Sbjct: 243 LVGIMVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEENNDVERIMRGENERKVERTDE 302

Query: 304 GEAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSS 363
           GEAMEEIGAKEMVKRINFWLY GVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSS
Sbjct: 303 GEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSS 362

Query: 364 FGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGTLCGGFFLLLSPSDTSLCMSTAIIA 423
           FGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMG LCG                     
Sbjct: 363 FGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCG--------------------- 422

Query: 424 ICTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLYHKQAGHGVDPG 483
              GAITSISVSTTTDLFGATNFSINHN+VVANIPFGSFIFGYMAAFLYHKQAGHGV  G
Sbjct: 423 ---GAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVAAG 482

Query: 484 KCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYSTTKKSSYIPSN 532
           KCIGVECYRTTFLIWG FSSFGTFLALLLFART+SFYSTT  SS+I  N
Sbjct: 483 KCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLSSHISPN 507

BLAST of CSPI01G24430 vs. ExPASy TrEMBL
Match: A0A6J1I3F0 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469568 PE=4 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 1.5e-220
Identity = 420/523 (80.31%), Postives = 459/523 (87.76%), Query Frame = 0

Query: 1   MPSSSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKL 60
           MPSS   VFQWLSL+GIIWLQSINGTNFNFPSYSSQLK  LS+SQ QLNNLAFASDAGKL
Sbjct: 1   MPSS---VFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKL 60

Query: 61  FACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSIC 120
           FAC SGLAANYLPLW VL IGSSLGL+GYGVQYLFITNQFHSPSY LIFFLTVLAGNSIC
Sbjct: 61  FACVSGLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSIC 120

Query: 121 WINTVCYMVAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLL 180
           WINTVCY+VAI NF  S+RQVAVGISTSYQGLSAK+YTDIVG IFS K S KTAETFLL 
Sbjct: 121 WINTVCYIVAIRNF-PSNRQVAVGISTSYQGLSAKIYTDIVGLIFS-KRSDKTAETFLLS 180

Query: 181 NSVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSA 240
           NSV PLGVCVLVSP+AR V++VEEQG LEVGFFVIFVITIATG+FA +TSVGS+SRMLS 
Sbjct: 181 NSVFPLGVCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSP 240

Query: 241 LGGLVGIMVFLVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEKNDEERMRGENERKVERT 300
           L GLVGI+VFL+LP+VV VVE VK+ V EGKE +VYH T+EE   + R+R ENE K+ R 
Sbjct: 241 LSGLVGILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEE---DTRIRPENEAKLNRD 300

Query: 301 DDGEAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360
              EAMEEIGA EM+KR+NFWLYVGVY FGATLGLAFLNNLGQIAESRGS SVSSLVSLS
Sbjct: 301 GGDEAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLS 360

Query: 361 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGTLCGGFFLLLSPSDTSLCMSTAI 420
           SSFGFFGRL+PS+LD+FLSRR K MKSKPGW+V LMG +CGGFFLL  P+D  LC+STA+
Sbjct: 361 SSFGFFGRLMPSLLDHFLSRR-KMMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVSTAV 420

Query: 421 IAICTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLYHKQAGHGVD 480
           IA+CTGAITSISVSTT++LFGA NFSINHN+VVANIP GSF+FGYMAA+LY KQA  G  
Sbjct: 421 IAVCTGAITSISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGGA- 480

Query: 481 PGKCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYSTTK 524
            GKCIGVECYRTTF+IWG   SFGT LALLLFART+SFYSTTK
Sbjct: 481 -GKCIGVECYRTTFMIWGCLCSFGTLLALLLFARTRSFYSTTK 512

BLAST of CSPI01G24430 vs. ExPASy TrEMBL
Match: A0A6J1HMC6 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464885 PE=4 SV=1)

HSP 1 Score: 760.4 bits (1962), Expect = 5.1e-216
Identity = 414/524 (79.01%), Postives = 456/524 (87.02%), Query Frame = 0

Query: 1   MPSSSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKL 60
           MPSS   VFQWLSL+GIIWLQSINGTNFNFPSYSS LK  LS+SQ QLNNLAFASDAGKL
Sbjct: 55  MPSS---VFQWLSLLGIIWLQSINGTNFNFPSYSSPLKHHLSISQFQLNNLAFASDAGKL 114

Query: 61  FACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSIC 120
           FAC SGLAANYLPLW +L IGS LGL+GYGVQYLFITNQFHSPSY LIFFL+VLAGNSIC
Sbjct: 115 FACVSGLAANYLPLWSLLIIGSCLGLVGYGVQYLFITNQFHSPSYCLIFFLSVLAGNSIC 174

Query: 121 WINTVCYMVAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLL 180
           WINTVCY+VAI NF  S+RQVAVGISTSYQGLSAK+YTDIVG IFS K S KTAETFLL 
Sbjct: 175 WINTVCYIVAIRNF-PSNRQVAVGISTSYQGLSAKIYTDIVGLIFS-KRSDKTAETFLLS 234

Query: 181 NSVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSA 240
           NSV PLGVCVLV P+AR V++VEE+G LEVGFFVIFVITIATG+FA +TSVGS+SRMLS 
Sbjct: 235 NSVFPLGVCVLVCPMARIVELVEEKGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSP 294

Query: 241 LGGLVGIMVFLVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEKNDEERMRGENERKVERT 300
           L GLVGI+VFL+LP+VV VVE VK+ V EGKE +VYH T+EE   + R+R ENE K+ R 
Sbjct: 295 LSGLVGILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEE---DTRIRPENEAKLNRD 354

Query: 301 DDGEAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360
              EAMEEIGA EM+KR+NFWLYVGVY FGATLGLAFLNNLGQIAES GS SVSSLVSLS
Sbjct: 355 GGDEAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESWGSESVSSLVSLS 414

Query: 361 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGTLCGGFFLLLS-PSDTSLCMSTA 420
           SSFGFFGRL+PS+LD+FLSRR K MKSKPGW+V LMG +CGGFF LLS P+D  LC+STA
Sbjct: 415 SSFGFFGRLMPSLLDHFLSRR-KMMKSKPGWLVALMGAMCGGFFFLLSGPTDLILCVSTA 474

Query: 421 IIAICTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLYHKQAGHGV 480
           +IA+CTGAITSISVSTT++LFGA NFSINHN+VVANIPFGSF+FGYMAA+LY KQA    
Sbjct: 475 VIAVCTGAITSISVSTTSELFGANNFSINHNVVVANIPFGSFLFGYMAAYLYRKQATG-- 534

Query: 481 DPGKCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYSTTK 524
           D GKCIGVECYRTTF+IWG   S GT LALLLFART+SFYSTTK
Sbjct: 535 DTGKCIGVECYRTTFMIWGCLCSLGTLLALLLFARTRSFYSTTK 567

BLAST of CSPI01G24430 vs. NCBI nr
Match: XP_011658866.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KGN65862.1 hypothetical protein Csa_023247 [Cucumis sativus])

HSP 1 Score: 1003.4 bits (2593), Expect = 7.2e-289
Identity = 530/531 (99.81%), Postives = 530/531 (99.81%), Query Frame = 0

Query: 1   MPSSSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKL 60
           MPSSSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKL
Sbjct: 1   MPSSSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKL 60

Query: 61  FACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSIC 120
           FACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSIC
Sbjct: 61  FACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSIC 120

Query: 121 WINTVCYMVAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLL 180
           WINTVCYMVAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLL
Sbjct: 121 WINTVCYMVAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLL 180

Query: 181 NSVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSA 240
           NSVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSA
Sbjct: 181 NSVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSA 240

Query: 241 LGGLVGIMVFLVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEKNDEERMRGENERKVERT 300
           LGGLVGIMVFLVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEKNDEERMRGENERKVERT
Sbjct: 241 LGGLVGIMVFLVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEKNDEERMRGENERKVERT 300

Query: 301 DDGEAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360
           DDGEAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS
Sbjct: 301 DDGEAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360

Query: 361 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGTLCGGFFLLLSPSDTSLCMSTAI 420
           SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGTLCGGFFLLLSPSDTSLCMSTAI
Sbjct: 361 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGTLCGGFFLLLSPSDTSLCMSTAI 420

Query: 421 IAICTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLYHKQAGHGVD 480
           IAICTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLY KQAGHGVD
Sbjct: 421 IAICTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLYRKQAGHGVD 480

Query: 481 PGKCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYSTTKKSSYIPSN 532
           PGKCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYSTTKKSSYIPSN
Sbjct: 481 PGKCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYSTTKKSSYIPSN 531

BLAST of CSPI01G24430 vs. NCBI nr
Match: XP_008457307.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo])

HSP 1 Score: 948.3 bits (2450), Expect = 2.7e-272
Identity = 505/529 (95.46%), Postives = 512/529 (96.79%), Query Frame = 0

Query: 4   SSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 63
           SSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC
Sbjct: 3   SSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 62

Query: 64  FSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWIN 123
           FSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWIN
Sbjct: 63  FSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWIN 122

Query: 124 TVCYMVAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLLNSV 183
           TVCYMVAITNF SS+RQVAVGISTSYQGLSAKVYTDIVGSIFSNK SSKTAETFLLLNSV
Sbjct: 123 TVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLNSV 182

Query: 184 LPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSALGG 243
           LPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATG FAT+TSVGSVSRMLSAL G
Sbjct: 183 LPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSALSG 242

Query: 244 LVGIMVFLVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEKNDEER-MRGENERKVERTDD 303
           LVGIMVFLVLPLVVVV EKVKERVEEGKEG+VYHFTVEE ND ER MRGENERKVERTD+
Sbjct: 243 LVGIMVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEENNDVERIMRGENERKVERTDE 302

Query: 304 GEAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSS 363
           GEAMEEIGAKEMVKRINFWLY GVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSS
Sbjct: 303 GEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSS 362

Query: 364 FGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGTLCGGFFLLLSPSDTSLCMSTAIIA 423
           FGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMG LCGGFFLLLSPSDTSLCMSTAIIA
Sbjct: 363 FGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTAIIA 422

Query: 424 ICTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLYHKQAGHGVDPG 483
           ICTGAITSISVSTTTDLFGATNFSINHN+VVANIPFGSFIFGYMAAFLYHKQAGHGV  G
Sbjct: 423 ICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVAAG 482

Query: 484 KCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYSTTKKSSYIPSN 532
           KCIGVECYRTTFLIWG FSSFGTFLALLLFART+SFYSTT  SS+I  N
Sbjct: 483 KCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLSSHISPN 531

BLAST of CSPI01G24430 vs. NCBI nr
Match: KAA0059993.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa] >TYJ97252.1 protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa])

HSP 1 Score: 891.7 bits (2303), Expect = 3.0e-255
Identity = 481/529 (90.93%), Postives = 488/529 (92.25%), Query Frame = 0

Query: 4   SSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 63
           SSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC
Sbjct: 3   SSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 62

Query: 64  FSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWIN 123
           FSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWIN
Sbjct: 63  FSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWIN 122

Query: 124 TVCYMVAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLLNSV 183
           TVCYMVAITNF SS+RQVAVGISTSYQGLSAKVYTDIVGSIFSNK SSKTAETFLLLNSV
Sbjct: 123 TVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLNSV 182

Query: 184 LPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSALGG 243
           LPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATG FAT+TSVGSVSRMLSAL G
Sbjct: 183 LPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSALSG 242

Query: 244 LVGIMVFLVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEKNDEER-MRGENERKVERTDD 303
           LVGIMVFLVLPLVVVV EKVKERVEEGKEG+VYHFTVEE ND ER MRGENERKVERTD+
Sbjct: 243 LVGIMVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEENNDVERIMRGENERKVERTDE 302

Query: 304 GEAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSS 363
           GEAMEEIGAKEMVKRINFWLY GVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSS
Sbjct: 303 GEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSS 362

Query: 364 FGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGTLCGGFFLLLSPSDTSLCMSTAIIA 423
           FGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMG LCG                     
Sbjct: 363 FGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCG--------------------- 422

Query: 424 ICTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLYHKQAGHGVDPG 483
              GAITSISVSTTTDLFGATNFSINHN+VVANIPFGSFIFGYMAAFLYHKQAGHGV  G
Sbjct: 423 ---GAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVAAG 482

Query: 484 KCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYSTTKKSSYIPSN 532
           KCIGVECYRTTFLIWG FSSFGTFLALLLFART+SFYSTT  SS+I  N
Sbjct: 483 KCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLSSHISPN 507

BLAST of CSPI01G24430 vs. NCBI nr
Match: XP_038895111.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Benincasa hispida])

HSP 1 Score: 837.4 bits (2162), Expect = 6.8e-239
Identity = 448/521 (85.99%), Postives = 482/521 (92.51%), Query Frame = 0

Query: 4   SSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 63
           SSSIVFQWLSL+GIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC
Sbjct: 2   SSSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 61

Query: 64  FSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWIN 123
           FSGLAANYLPLWLVLFIG+SLGL+GYGVQYLF+TNQ  SPSY +IF LTVLAGNSICWIN
Sbjct: 62  FSGLAANYLPLWLVLFIGASLGLVGYGVQYLFVTNQIQSPSYCVIFLLTVLAGNSICWIN 121

Query: 124 TVCYMVAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLLNSV 183
           TVCYMVAITNF  ++RQ+AVGISTSYQGLSAK+YT+IV SIFS KHSSKT ETFLLLNSV
Sbjct: 122 TVCYMVAITNF-GTNRQIAVGISTSYQGLSAKIYTNIVSSIFS-KHSSKTTETFLLLNSV 181

Query: 184 LPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSALGG 243
           LP+GVCVL SPLAR +K+VEEQGKLEVGFFVIFVITIATGIFA +TSVGS+SRMLS L G
Sbjct: 182 LPMGVCVLASPLARILKVVEEQGKLEVGFFVIFVITIATGIFAAVTSVGSISRMLSPLSG 241

Query: 244 LVGIMVFLVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEKNDEERMRGENERKVERTDDG 303
           LVGI+VFLVLPLVV VVEKVKE ++EGKE +VYHFTVEE  D ER+RGEN+ K+ER  D 
Sbjct: 242 LVGIVVFLVLPLVVAVVEKVKESLKEGKERRVYHFTVEENKDVERVRGENQSKLERDGDS 301

Query: 304 -EAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSS 363
            EAMEEIGAKEMVKRINFWLY GVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSS
Sbjct: 302 EEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSS 361

Query: 364 FGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGTLCGGFFLLLSPSDTSLCMSTAIIA 423
           FGFFGRL+PSILDYFLSR+ K MKSKPGWMVGLMG LCGGFFLLL P+D SLC+STAIIA
Sbjct: 362 FGFFGRLMPSILDYFLSRK-KCMKSKPGWMVGLMGVLCGGFFLLLCPTDVSLCVSTAIIA 421

Query: 424 ICTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLYHKQAGHGVDPG 483
           +CTGAITSISVSTT++LFGA NFSINHN++VANIPFGSF+FGYMAAFLYHKQAG G   G
Sbjct: 422 VCTGAITSISVSTTSELFGANNFSINHNLLVANIPFGSFLFGYMAAFLYHKQAG-GDGTG 481

Query: 484 KCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYSTTK 524
           KCIGVECYRTTF IWG FSSFGT LALLLFARTK+FYSTT+
Sbjct: 482 KCIGVECYRTTFFIWGCFSSFGTLLALLLFARTKTFYSTTR 518

BLAST of CSPI01G24430 vs. NCBI nr
Match: XP_022970645.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 775.4 bits (2001), Expect = 3.2e-220
Identity = 420/523 (80.31%), Postives = 459/523 (87.76%), Query Frame = 0

Query: 1   MPSSSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKL 60
           MPSS   VFQWLSL+GIIWLQSINGTNFNFPSYSSQLK  LS+SQ QLNNLAFASDAGKL
Sbjct: 1   MPSS---VFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKL 60

Query: 61  FACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSIC 120
           FAC SGLAANYLPLW VL IGSSLGL+GYGVQYLFITNQFHSPSY LIFFLTVLAGNSIC
Sbjct: 61  FACVSGLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSIC 120

Query: 121 WINTVCYMVAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLL 180
           WINTVCY+VAI NF  S+RQVAVGISTSYQGLSAK+YTDIVG IFS K S KTAETFLL 
Sbjct: 121 WINTVCYIVAIRNF-PSNRQVAVGISTSYQGLSAKIYTDIVGLIFS-KRSDKTAETFLLS 180

Query: 181 NSVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSA 240
           NSV PLGVCVLVSP+AR V++VEEQG LEVGFFVIFVITIATG+FA +TSVGS+SRMLS 
Sbjct: 181 NSVFPLGVCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSP 240

Query: 241 LGGLVGIMVFLVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEKNDEERMRGENERKVERT 300
           L GLVGI+VFL+LP+VV VVE VK+ V EGKE +VYH T+EE   + R+R ENE K+ R 
Sbjct: 241 LSGLVGILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEE---DTRIRPENEAKLNRD 300

Query: 301 DDGEAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360
              EAMEEIGA EM+KR+NFWLYVGVY FGATLGLAFLNNLGQIAESRGS SVSSLVSLS
Sbjct: 301 GGDEAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLS 360

Query: 361 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGTLCGGFFLLLSPSDTSLCMSTAI 420
           SSFGFFGRL+PS+LD+FLSRR K MKSKPGW+V LMG +CGGFFLL  P+D  LC+STA+
Sbjct: 361 SSFGFFGRLMPSLLDHFLSRR-KMMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVSTAV 420

Query: 421 IAICTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLYHKQAGHGVD 480
           IA+CTGAITSISVSTT++LFGA NFSINHN+VVANIP GSF+FGYMAA+LY KQA  G  
Sbjct: 421 IAVCTGAITSISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGGA- 480

Query: 481 PGKCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYSTTK 524
            GKCIGVECYRTTF+IWG   SFGT LALLLFART+SFYSTTK
Sbjct: 481 -GKCIGVECYRTTFMIWGCLCSFGTLLALLLFARTRSFYSTTK 512

BLAST of CSPI01G24430 vs. TAIR 10
Match: AT2G30300.1 (Major facilitator superfamily protein )

HSP 1 Score: 408.3 bits (1048), Expect = 9.5e-114
Identity = 253/527 (48.01%), Postives = 336/527 (63.76%), Query Frame = 0

Query: 8   VFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGL 67
           +  WLSL+ I+WLQS NGTN +FP+YSSQLK+ L +SQ +LN L+FASDAGK+    SG+
Sbjct: 5   ILPWLSLVAILWLQSFNGTNLSFPAYSSQLKELLKISQFKLNYLSFASDAGKVLGFISGI 64

Query: 68  AANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWINTVCY 127
           AA YLPL LVL  G SLG  GYG+QYL I  +                   ICWINT CY
Sbjct: 65  AAVYLPLPLVLLAGGSLGFAGYGLQYLSIIKKI------------------ICWINTACY 124

Query: 128 MVAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAET--FLLLNSVLP 187
           +VAI +F   +RQVAVGI+ SYQGLS K+YTD+V S F   H+S+  E   +LLLNS++P
Sbjct: 125 IVAINSF-PVNRQVAVGITASYQGLSGKIYTDMVHSFF---HTSQREEASGYLLLNSLVP 184

Query: 188 LGVCVLVSPLAR---FVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSALG 247
           L  C++ +P+       K +     ++VGF V+FV+TIATGI+A  TS+ SV  +L    
Sbjct: 185 LVACLVTAPMLMRHGGDKTMSYSKDVKVGFIVLFVLTIATGIYAVATSLVSVPAVLV--- 244

Query: 248 GLVGIMVFLVLPLVVVVVEKVKERVEEGKEGKVYH---------FTVEEKNDEERMRGEN 307
            LVGI +FL+ PL + +    KE +   K  +  H         + VEE + +E      
Sbjct: 245 -LVGIALFLLAPLAIPIGVGFKELMSSRKTQQKVHDLEAPVDKFYFVEEDHTKE------ 304

Query: 308 ERKVERTDDGEAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSV 367
           E + E+   G   EE+   ++ K+++FW+Y G+YLFG T+GL F NNLGQIAESRGS++ 
Sbjct: 305 EEEFEKAIIG-VKEEVEWTQLWKKLDFWIYFGLYLFGPTVGLVFTNNLGQIAESRGSTAT 364

Query: 368 SSLVSLSSSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGTLCGGFFLLLSPSDTS 427
           SSLV+LSSSFGFFGRLLPS+LDYF S RNK+M S P  M G +  +   F LLL  SD +
Sbjct: 365 SSLVALSSSFGFFGRLLPSLLDYFFS-RNKYMPSSPVSMAGSLVAMVASFLLLLIDSDIA 424

Query: 428 LCMSTAIIAICTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLYHK 487
           L + TA+I I +GA+TS+SV+ T +LFG  +F +NHNIVV +IP GSF FG +AA +Y  
Sbjct: 425 LYIGTAMIGIFSGALTSLSVTMTAELFGTKHFGVNHNIVVGSIPLGSFSFGLLAAKIYRD 484

Query: 488 QAGHGVDPGKCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYS 521
            A    D GKC G+ C++TT + WG   S    LA +L+ R + FYS
Sbjct: 485 GAALYGDDGKCFGMHCFQTTLVFWGMLCSIAALLAAVLYIRNRKFYS 497

BLAST of CSPI01G24430 vs. TAIR 10
Match: AT1G31470.1 (Major facilitator superfamily protein )

HSP 1 Score: 272.3 bits (695), Expect = 8.1e-73
Identity = 194/536 (36.19%), Postives = 292/536 (54.48%), Query Frame = 0

Query: 10  QWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 69
           +W  L+  IW+Q+  GTNF+F +YSS LK  L +SQ++LN LA ASD GK F   SG+A 
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 70  NYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWINTVCYMV 129
            Y PL +VLF  +++G +GYGVQ+L ITN    P Y L+F   +LAG SICW NT C+++
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFNTACFIL 162

Query: 130 AITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLLNSVLPLGVC 189
            I +F  ++R +A+ ++ S+ G+SA +Y     S+  N  +  ++  +LLLNS++PL V 
Sbjct: 163 CIRHF-PNNRALALSLTVSFNGISAALY-----SLAFNAINPSSSNLYLLLNSLVPLVVS 222

Query: 190 ------VLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSALGG 249
                 VL  P        + +      F ++ V+ + T     ++S  + S  L+ +G 
Sbjct: 223 FAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIGA 282

Query: 250 LVGIMVFLVLPLVVVV----VEKVKERVEEGKEG---------KVYHFTVEEKNDEERMR 309
           +V ++  L  PL+V      +  +  R+     G         K    +V  K   E M 
Sbjct: 283 VVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMG 342

Query: 310 GENERKVERTDDGEAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGS 369
              E    R  D     E   + ++ R+ FWLY   Y  G T+GL + NNLGQIA+S G 
Sbjct: 343 TAKEGNTVRLGD-----EHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQ 402

Query: 370 SSVSSLVSLSSSFGFFGRLLPSILDYFLSRRNKFMKSKPGWM-VGLMGTLCGGFFLLLSP 429
           +S ++LV++ SSF FFGRLL +  D+      +F  ++ GW  + L+ T    F L +S 
Sbjct: 403 NS-TTLVTIYSSFSFFGRLLSAAPDFM---HKRFRLTRTGWFAIALLPTPIAFFLLAVSS 462

Query: 430 S-DTSLCMSTAIIAICTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAA 489
           S  T+L  +TA+I + +G I + +VS T+DLFG  +  +NHNI++ NIP GS ++GY+AA
Sbjct: 463 SQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGYIAA 522

Query: 490 FLYHKQAGHGVDP-----GKCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFY 520
            +Y   A   + P       CIG +CY  TF+ WG  S  G   +L L+ RTK  Y
Sbjct: 523 SIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of CSPI01G24430 vs. TAIR 10
Match: AT5G45275.1 (Major facilitator superfamily protein )

HSP 1 Score: 263.5 bits (672), Expect = 3.8e-70
Identity = 196/560 (35.00%), Postives = 307/560 (54.82%), Query Frame = 0

Query: 10  QWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 69
           +W+ L+  IW+Q+  GTNF+F +YSS LK  L +SQ+QLN LA ASD GK+F   SGLA 
Sbjct: 7   KWMILVATIWIQAFTGTNFDFSTYSSNLKSVLGISQVQLNYLAVASDLGKVFGWSSGLAL 66

Query: 70  NYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWINTVCYMV 129
            Y PLW VLF  + +G +GYGVQ+L ITN    P Y L+F   +LAG SICW NTVC+++
Sbjct: 67  LYFPLWTVLFAAAIMGFVGYGVQWLVITNVISLP-YILVFLCCLLAGLSICWFNTVCFVL 126

Query: 130 AITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLLNSVLPLGVC 189
            I NF  ++R +A+ ++ S+ G+SA +YT     +  N  +  + E +LLLN+++PL V 
Sbjct: 127 CIRNF-PANRSLALSLTVSFNGVSAALYT-----LAYNAINPVSTELYLLLNALVPLFVS 186

Query: 190 -VLVSPLARFVKI----VEEQGKLEVGFFVIFVITIATGI----FATMTSVGSVSRMLSA 249
              + P+ R   +     +   +  + F ++ ++ +  G+    F + TS  + +R+L  
Sbjct: 187 FAALIPILRQPPLEPLPPDGVRRDSLMFLLLNILAVLNGVYLLLFRSKTSDVTSARLLFG 246

Query: 250 LGGLVGIMVFLVLPLVV---------------------VVVEKVKERVEEG---KEGKVY 309
            G ++ +++ L LP +V                     ++V+  +  + +G   +E  + 
Sbjct: 247 -GSILLLILPLCLPGLVYARNWYLHNIHSSFRLEGSGFILVDVDELEMHKGMVTREASLE 306

Query: 310 HFTVEEKNDEERMRGENERKVERTDDGEAM-------------EEIGAKEMVKRINFWLY 369
            + +   +    +   +++     DDG                EE     ++ R +FWLY
Sbjct: 307 GYQLLNDDVVRAVNTPDQKSFIEDDDGCCCTKVITRNQLGMLGEEHPLSFLLCRSDFWLY 366

Query: 370 VGVYLFGATLGLAFLNNLGQIAESRG-SSSVSSLVSLSSSFGFFGRLLPSILDYFLSRRN 429
              Y  G T+GL + NNLGQIA+S G SS  ++LV+L SSF FFGRLL +  DY    R 
Sbjct: 367 YIAYFCGGTIGLVYSNNLGQIAQSLGQSSETTTLVTLYSSFSFFGRLLSATPDYI---RA 426

Query: 430 KFMKSKPGWM-VGLMGTLCGGFFLLLSPSDTSLCMSTAIIAICTGAITSISVSTTTDLFG 489
           K   ++ GW+ V L+ T    F L  S S  +L   TA+I + +G I + +VS T++LFG
Sbjct: 427 KVYFARTGWLAVALLPTTIALFLLASSGSLAALQAGTALIGLSSGFIFAAAVSITSELFG 486

Query: 490 ATNFSINHNIVVANIPFGSFIFGYMAAFLY--HKQAGHGVDPGKCIGVECYRTTFLIWGS 520
             +  +NHNI++ NIP GS ++G++AA +Y  H  AG   +   C+G +CY  TF+ WG 
Sbjct: 487 PNSVGVNHNILITNIPIGSLVYGFLAALVYESHSVAGSKTESVICMGRDCYLQTFMWWGC 546

BLAST of CSPI01G24430 vs. TAIR 10
Match: AT4G19450.1 (Major facilitator superfamily protein )

HSP 1 Score: 258.5 bits (659), Expect = 1.2e-68
Identity = 197/563 (34.99%), Postives = 310/563 (55.06%), Query Frame = 0

Query: 10  QWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 69
           +W+ L+  IW+Q+  GTNF+F +YSS LK  L +SQ+QLN LA ASD GK+F   SGLA 
Sbjct: 7   KWMILVATIWIQAFTGTNFDFSAYSSDLKSVLGISQVQLNYLAVASDLGKVFGWSSGLAL 66

Query: 70  NYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWINTVCYMV 129
            Y PLW VLF  + +G +GYGVQ+L IT+ F S  Y ++F   +LAG SICW NTVC+++
Sbjct: 67  MYFPLWTVLFAAAFMGFVGYGVQWLVITH-FISLPYIMVFLCCLLAGLSICWFNTVCFVL 126

Query: 130 AITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLLNSVLPLGVC 189
            I+NF  ++R +A+ ++ S+ G+SA +YT     +  N  +  + E +LLLN+++PL V 
Sbjct: 127 CISNF-PANRSLALSLTVSFNGVSAALYT-----LAYNAINPTSPELYLLLNALIPLIVS 186

Query: 190 -VLVSPLAR---FVKIVEEQGKLEVGFFVIFVITIATG-----IFATMTSVGSVSRMLSA 249
              + P+ R   F  +  +  + +   F++  I  A       +F + +S  + +R+L  
Sbjct: 187 FTAIIPILRQPPFEPLPPDGVRRDSLMFLLLNILAALNGVYLLLFGSNSSDLTSARLL-- 246

Query: 250 LGGLVGIMVF-LVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEKNDEERMRG-------- 309
            GG + ++VF L +P +V+        +      +   F + + ++ E  +G        
Sbjct: 247 FGGAILLLVFPLCIPGLVIARNWYNRTIHTSFRLEGSGFILVDPDELELHKGMLAHEANR 306

Query: 310 -----------ENERK---VERTDDGEA----------MEEIGAKE----MVKRINFWLY 369
                      +N  K   VE  D  E+          +E +G +     ++ R +FWLY
Sbjct: 307 EGYQLLSDDVVQNPVKSVAVEEEDSDESCCKKLITRDQLEGLGIEHSLSLLLTRSDFWLY 366

Query: 370 VGVYLFGATLGLAFLNNLGQIAESRG-SSSVSSLVSLSSSFGFFGRLLPSILDYFLSRRN 429
              Y  G T+GL + NNLGQIA+S G SS+ ++LV+L S+F FFGRLL +  DY    R 
Sbjct: 367 YITYFCGGTIGLVYSNNLGQIAQSLGQSSNTTTLVTLYSAFSFFGRLLSATPDYI---RA 426

Query: 430 KFMKSKPGWM-VGLMGTLCGGFFLLLSPSDTSLCMSTAIIAICTGAITSISVSTTTDLFG 489
           K   ++ GW+ + L+ T    F L  S + ++L   TA++ + +G I + +VS T++LFG
Sbjct: 427 KVYFARTGWLAIALLPTPFALFLLASSGTASALQAGTALMGLSSGFIFAAAVSITSELFG 486

Query: 490 ATNFSINHNIVVANIPFGSFIFGYMAAFLYHKQAGHGV-----DPGKCIGVECYRTTFLI 520
             +  +NHNI++ NIP GS I+G++AA +Y      G      +   C+G +CY  TF+ 
Sbjct: 487 PNSVGVNHNILITNIPIGSLIYGFLAALVYDSHGFTGTKSMTSESVVCMGRDCYYLTFVW 546

BLAST of CSPI01G24430 vs. TAIR 10
Match: AT3G01630.1 (Major facilitator superfamily protein )

HSP 1 Score: 251.1 bits (640), Expect = 1.9e-66
Identity = 185/533 (34.71%), Postives = 285/533 (53.47%), Query Frame = 0

Query: 10  QWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 69
           +W  L+  IW+Q+  GTNF+F +YSS +K  + +SQ +LN +A ASD GK     SG A 
Sbjct: 23  RWTVLVAAIWIQAFTGTNFDFSAYSSDMKSSMGVSQSRLNYMAVASDLGKALGWSSGFAI 82

Query: 70  NYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWINTVCYMV 129
            Y P+  VLF  +++GL+GYGVQ+L I +    P Y L+     LAG SICW NTV +++
Sbjct: 83  AYFPVSGVLFAAAAMGLVGYGVQWLSIADVIDLP-YSLVLVCCSLAGLSICWFNTVSFIL 142

Query: 130 AITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLLNSVLPLGVC 189
            I +FK ++  +A+ +  S+ G+SA +YT  +G    +  SS +++ +LLLNS++PL V 
Sbjct: 143 CIRHFK-ANHSLALSLVVSFNGISAALYT--LGHEAISGKSSASSDIYLLLNSLIPLIVS 202

Query: 190 ------VLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSALGG 249
                 VL +P +          +  + F V  V+ + T  +  + S G+     S    
Sbjct: 203 VLALWPVLTNPNSSETDTTRTHDETRI-FVVFNVLALVTCFYLLLPSSGTYLAS-SPRWH 262

Query: 250 LVGIMVFLVLPLVVVVVEKVKERVEE--GKEGKVYHFTVEEKNDEERMRGENERKVERTD 309
            +G +  L+ PL V  ++ +   +E         Y     E+    +++ +     E +D
Sbjct: 263 FLGAIFLLLFPLCVPFLDYIHRALESCFHHHSSGYAAVNIEEPKILKIKSQKSNAEEESD 322

Query: 310 DGEAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRG--SSSVSSLVSL 369
                +E     +V+++ FWLY   Y  G T+GL + NNLGQIA+S G  SS+  SLV+L
Sbjct: 323 QVRLGDEHSLGMLVRKLEFWLYYVAYFCGGTIGLVYSNNLGQIAQSLGQSSSNAKSLVTL 382

Query: 370 SSSFGFFGRLLPSILDYFLSRRNKFMKSKPGWM-VGLMGTLCGGFFLLLSPSDTS---LC 429
            S+F F GRLL S  D F  ++ K++ ++ GW  + L+ T    F L  SP       L 
Sbjct: 383 FSAFSFLGRLLSSAPD-FTRKKLKYL-TRTGWFTISLLPTPLAFFILAYSPKTNQTALLE 442

Query: 430 MSTAIIAICTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLYHKQA 489
           ++TA+I + +G + + +VS T++LFG  +  +N NI++ NIP GS  +GYMA  +Y   A
Sbjct: 443 VATALIGLSSGFVFAAAVSITSELFGRNSVGVNQNILITNIPIGSLFYGYMAGSVYDTNA 502

Query: 490 GHG-----VDPGKCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYSTTK 524
             G      D   C+G +CY  TFL WG  S  G   +L LF RT+  Y   K
Sbjct: 503 SLGRKSVVADSVVCVGRKCYFVTFLFWGCLSVLGFVCSLFLFIRTRPVYHRLK 547