CSPI01G24420 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G24420
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	PseudoU_synth_1 domain-containing protein
Location	Chr1: 19707478 .. 19711487 (+)
RNA-Seq Expression	CSPI01G24420
Synteny	CSPI01G24420

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCCTGAGTTTCCTAAAATGTCTGTCCATCGGAAATCTGATAAGATCAGTTAAAGCTGATAGGATCCATCTAAGTTGGCTAACGGAGAGAGATGCTTCTGCCGGCTTCTACATCTTCCTTCTTGCCGGTCAATTTTACAACTAACAACCTCCGTAATGTGCTCTGAGACTTAACGGAGAGGACTGCTGAAAAACCTGGCTGCCAGAGAGGGAAGGAGAGGGGAGGTTGGTGATGGTCAACAGGGAATAATAAGATGTAAGCCTTATTCCTTTGCCCTTGTATATTCTTTCATCTATCTTGATGAAGTTTGTTTGTTTCTAATATATAAGGACTCGTCAATGACTCAATAGAGCATTCATAGTTTAAGCTATTTTTTCAACTTGCACTTGAAACTTTCACAAGAGGAACTTTCAATCCATCTTCTTATGATGGACTGACGGATTGGGTTCCATGTACTGAAGACAGTGCATTCTAATAGGCAAATTTTCAAGAAGTGTTAAACAATCTTGCTGGTACATGCTTTGGGAGAACTAAAGACGTGTGGCAGAATAGTTACTGCTAAAAGAGTTTATGAAAAGGATTATAAACATTACCAATGCCATTAAATTTTGTTCTCACCATATGTCAGTTCCTAATTACAACTATTTCATTACAATCTAGTTGCATAATGATTGACTGAAATAATCGAACTTAGTATGTATGTCACCAGATCAATAACTGATTAAATACACGTAAACTGATTATCCACTTAACCATTTAACTAACCTCGACATTTATATATATATTACACTTCTCTAAGTATCCTAGATAAGCATTTTTTTTCCTTTCAATGTTTCTTTTACCATGGCATCAAACAATATTAAAGGGTTTCCATTGGCTATGATAATAAAGTACTAAGGCATGGAATATGATAATACTGTGTAAAGACTCTTTTTTTCTTCCTTTTGCTGGGAATTACAACGCAGAAAATTAACCTCATAAAGTTTTGGTGTATAGCAATCGAACAAGAATTGGAAGATGCAATATATAAAGCTGGTGGAATTTGTGATAGTAACTACGGAAATCTGAACAAAATCTCATGGGCAAGGAGTAGCCGCACTGATAAAGGAGTAAATATCTTGAATGTCTTAGTACCTCATTTTATGACTTCTATAAACTTATTGGTTGTTTTGAATTGTTATTTCATCTCTTTCTAATTCAAGGTTCATTCTTTGGCTACAACAATAACCTTAAAAATGGAAATCCCTGAAAGTGCATGGATAGACGACCCTTGTGGTATTTCACTTGCTGATTACATCAACTCTTATCTTCCTGATAATATCAAGGTTTTTGGCATTGTACCTGCACAGAAGTGAGTGTTCTGAGGTTTTTTCATTGGTGCTTTTTAAGTGAGTAAATGAAGTGTTAGCACTAGCATGATACACTTTTACGCATAAACTACAAAGTGGTAACATTGCATCAATTTTGTTACTTGTCAAGGAGGTTTGATCCTAGAAAGGAGTGTGATGTTCGGATGTATTCGTATCTCCTCCCTGCTAAGCTTATTGGAATTGAAAGTAAACTTAAAACTACTGAAATTGATTGCCACATCTCAGACTTTAAAGATATATTAAAATCCTTTGAGGTACGTTATGATCTCCTTCAATTAATTACATACGCATCATATTGCCCCTTCCTTCACTGCAAGAAACATGATTTGTTATTTGATACAGTATAGTGCTGTTATTTGGGAAGACAATTATATGTCGTTGAAGAACAAAGGTGAATTCTTTTTGGCCACAGGGGGACCATCCTTTTCATAATTATACAGTACGCTCCATTTATAGAACGAGAATTCGAGCCAAGCAACTAGTATATGCCCTTAAGTCAGGCAAGAAGGTATCACCCGGTGATATGTCAAACTCTGAGTTTGAGGGAAGTGATCAAGAAAATGATACTTTTGTTGAAGGAATCAAGGAACCAGATCAACGTTCTCCAAGCTTAAGCGTCTGTGAAAATCTAACTGAAGTATGCAGTAAACTTGAAGATGGATCAGAACTTCTGAAGGGTGTTCGTGCAAGATGGCTTCATGAGCCAGATGAGAAAGATCGTATAGGCGCCTCACACTTCAGAAGGATTCTTTACTGTTCTTGTGGGAAGCTGGAAACATATTTTGGATGTGATTTCATTGAGATATCCATTTGGGGAGAATCATTCATGTTGCATCAAGTTAGTCTTTCTCTAACATTGTTTTTATGGTACATAATTTGTTCTCATCCATTTTCATTCCAATTTGTAAGTTAATCTATATTTACTCCATTTATTCTTAATTTCAGTTGGTAGCTCTATTGCTTAGATAGGTTTTCCGTTGTAATGCCTCTGGTAGCTTATATCTTGCTTCTGGCCATGTGGGAGCCAAAATTTGGTTTTATAACTGTACTTTGGTGCTCACTCGACAACTTTTTTGCTGCATTATTTCACTTTTTAGACTCTTTTCCCTCTTTGGAAATCAATGCTGGCTTAATGGACTTTTTCCTGTGCTAGCAGTCCAAAAAATTAAGAAATAGCTATGGGTTTATGTTTTAGGAAATTAAATTAAATTCTAAAATATTACCATCGATTAAATAATTTGATGGTCCTATCCATTCCTGGACGAAAGATAGTAGTTAATGGGTTCAAGTTTTATGAGCTTGACTTGTTCAATATCTACCATTATAACCTTCATTTACTTCCTGAGGTTCTAAATTTGTAGGATCAGCATGGTCCCTGTGCATTGGTGGACAAAAGATATGAGTTGATGGGTTCAAGCTTTATGAGCTTGACTTGTTAACTATATACCATCATAACCTTCATTTACTCCCTAAGGTTCATAATTCATGAGTTCAAGCTTTATGAGAAACTAGAATCTATTATTAAGTTTAGGAATTGGAAGGAGAGTGGGAGGTGAAAAGGTGTCAGGCTTTCTTTTGGCAGATTTCTCATTTCCATTCTTTTTGTAACTTTTGAGCTGATAATATGATATTTTCTTATTAAAAAAGAACATCAATAACATATCTTATGAACAATGAAGTTGTTTTCATTAGTAATTAATAAAGGGTAGTTAATGTTCTAGTTTTAAACCGAAAGGACCATTCTGATGCAACTTTTATTACATCCTTGGCAGATTCGGAAAATGATAGCAACGGCTGTGGCAGTGAAGCGGAAACTTCTCCCAAGAGATATTATAACATTGTCACTGACCAAGTTTTCTAGAATAGTTTTACCCATTGCTCCAGCTGAGGTCTTAGTCCTAAGAGGGAACAGTTTTTCCAACAGAAAACGGCCAGGAGCTCCAATGAGGTCAGAGTTGTTCACACTGAATGAATCAGATGACATAGTCAATTCAGTGAATGAATTCTACAGATCAGCGATATTGCCCGAGGTAACTAAGTTCCTGGACACAGAAAGCCCCCCATGGAAAGAATGGGTGGAGAAATTGGACAAGTACACAAGCATTCCGAATGAAGAATTGGATGAAGTTAGGAGTGCTTGGAAAGCATGGAAGTACAGGGAACATTGATCTGATCATGAGAAATCAAGATGGCCTTTGAATTATGTTGATGTGTTTTTTTGTCTGCCCCTCTGTTGTTGGAAGTCGTCAAAAACTTGAATGTTGAAGAAGGATGGGAAGCAGTTGAGTTTGAGTTAGGGGAGTTATTAATGGCATAAATTATGGGAATTGGGCATTGAATGGACGTGGTCTGTGGCCACCCACCAGCCGGGGGGCGTTTTAATAAGCTCAGCATCAACTGCAGACAATCAACTGGGGAATTGGCACAAGCCACAAGGGAGTGAGCTCACATTCAATGTAAATGGCTTAAAAGGCGAAATATGTAGGTGAGCCTTTGACACAGGGCTGTACTGATGTTGATTCAACTTCATTTCTGATGGAAATTTGGATCTAAACTTTGACTAATACCCATTATGAACATTTCATGTTGCTTTCCCTCTTAATTTACAATGATATACTGTTTATTGTTTTATTTGTT

mRNA sequence

ATGTCCCTGAGTTTCCTAAAATGTCTGTCCATCGGAAATCTGATAAGATCAGTTAAAGCTGATAGGATCCATCTAAGTTGGCTAACGGAGAGAGATGCTTCTGCCGGCTTCTACATCTTCCTTCTTGCCGACTTAACGGAGAGGACTGCTGAAAAACCTGGCTGCCAGAGAGGGAAGGAGAGGGGAGCAATCGAACAAGAATTGGAAGATGCAATATATAAAGCTGGTGGAATTTGTGATAGTAACTACGGAAATCTGAACAAAATCTCATGGGCAAGGAGTAGCCGCACTGATAAAGGAGTTCATTCTTTGGCTACAACAATAACCTTAAAAATGGAAATCCCTGAAAGTGCATGGATAGACGACCCTTGTGGTATTTCACTTGCTGATTACATCAACTCTTATCTTCCTGATAATATCAAGGTTTTTGGCATTGTACCTGCACAGAAGAGGTTTGATCCTAGAAAGGAGTGTGATGTTCGGATGTATTCGTATCTCCTCCCTGCTAAGCTTATTGGAATTGAAAGTAAACTTAAAACTACTGAAATTGATTGCCACATCTCAGACTTTAAAGATATATTAAAATCCTTTGAGGGGGACCATCCTTTTCATAATTATACAGTACGCTCCATTTATAGAACGAGAATTCGAGCCAAGCAACTAGTATATGCCCTTAAGTCAGGCAAGAAGGTATCACCCGGTGATATGTCAAACTCTGAGTTTGAGGGAAGTGATCAAGAAAATGATACTTTTGTTGAAGGAATCAAGGAACCAGATCAACGTTCTCCAAGCTTAAGCGTCTGTGAAAATCTAACTGAAGTATGCAGTAAACTTGAAGATGGATCAGAACTTCTGAAGGGTGTTCGTGCAAGATGGCTTCATGAGCCAGATGAGAAAGATCGTATAGGCGCCTCACACTTCAGAAGGATTCTTTACTGTTCTTGTGGGAAGCTGGAAACATATTTTGGATGTGATTTCATTGAGATATCCATTTGGGGAGAATCATTCATGTTGCATCAAATTCGGAAAATGATAGCAACGGCTGTGGCAGTGAAGCGGAAACTTCTCCCAAGAGATATTATAACATTGTCACTGACCAAGTTTTCTAGAATAGTTTTACCCATTGCTCCAGCTGAGGTCTTAGTCCTAAGAGGGAACAGTTTTTCCAACAGAAAACGGCCAGGAGCTCCAATGAGGTCAGAGTTGTTCACACTGAATGAATCAGATGACATAGTCAATTCAGTGAATGAATTCTACAGATCAGCGATATTGCCCGAGGTAACTAAGTTCCTGGACACAGAAAGCCCCCCATGGAAAGAATGGGTGGAGAAATTGGACAAGTACACAAGCATTCCGAATGAAGAATTGGATGAAGTTAGGAGTGCTTGGAAAGCATGGAAGTACAGGGAACATTGATCTGATCATGAGAAATCAAGATGGCCTTTGAATTATGTTGATGTGTTTTTTTGTCTGCCCCTCTGTTGTTGGAAGTCGTCAAAAACTTGAATGTTGAAGAAGGATGGGAAGCAGTTGAGTTTGAGTTAGGGGAGTTATTAATGGCATAAATTATGGGAATTGGGCATTGAATGGACGTGGTCTGTGGCCACCCACCAGCCGGGGGGCGTTTTAATAAGCTCAGCATCAACTGCAGACAATCAACTGGGGAATTGGCACAAGCCACAAGGGAGTGAGCTCACATTCAATGTAAATGGCTTAAAAGGCGAAATATGTAGGTGAGCCTTTGACACAGGGCTGTACTGATGTTGATTCAACTTCATTTCTGATGGAAATTTGGATCTAAACTTTGACTAATACCCATTATGAACATTTCATGTTGCTTTCCCTCTTAATTTACAATGATATACTGTTTATTGTTTTATTTGTT

Coding sequence (CDS)

ATGTCCCTGAGTTTCCTAAAATGTCTGTCCATCGGAAATCTGATAAGATCAGTTAAAGCTGATAGGATCCATCTAAGTTGGCTAACGGAGAGAGATGCTTCTGCCGGCTTCTACATCTTCCTTCTTGCCGACTTAACGGAGAGGACTGCTGAAAAACCTGGCTGCCAGAGAGGGAAGGAGAGGGGAGCAATCGAACAAGAATTGGAAGATGCAATATATAAAGCTGGTGGAATTTGTGATAGTAACTACGGAAATCTGAACAAAATCTCATGGGCAAGGAGTAGCCGCACTGATAAAGGAGTTCATTCTTTGGCTACAACAATAACCTTAAAAATGGAAATCCCTGAAAGTGCATGGATAGACGACCCTTGTGGTATTTCACTTGCTGATTACATCAACTCTTATCTTCCTGATAATATCAAGGTTTTTGGCATTGTACCTGCACAGAAGAGGTTTGATCCTAGAAAGGAGTGTGATGTTCGGATGTATTCGTATCTCCTCCCTGCTAAGCTTATTGGAATTGAAAGTAAACTTAAAACTACTGAAATTGATTGCCACATCTCAGACTTTAAAGATATATTAAAATCCTTTGAGGGGGACCATCCTTTTCATAATTATACAGTACGCTCCATTTATAGAACGAGAATTCGAGCCAAGCAACTAGTATATGCCCTTAAGTCAGGCAAGAAGGTATCACCCGGTGATATGTCAAACTCTGAGTTTGAGGGAAGTGATCAAGAAAATGATACTTTTGTTGAAGGAATCAAGGAACCAGATCAACGTTCTCCAAGCTTAAGCGTCTGTGAAAATCTAACTGAAGTATGCAGTAAACTTGAAGATGGATCAGAACTTCTGAAGGGTGTTCGTGCAAGATGGCTTCATGAGCCAGATGAGAAAGATCGTATAGGCGCCTCACACTTCAGAAGGATTCTTTACTGTTCTTGTGGGAAGCTGGAAACATATTTTGGATGTGATTTCATTGAGATATCCATTTGGGGAGAATCATTCATGTTGCATCAAATTCGGAAAATGATAGCAACGGCTGTGGCAGTGAAGCGGAAACTTCTCCCAAGAGATATTATAACATTGTCACTGACCAAGTTTTCTAGAATAGTTTTACCCATTGCTCCAGCTGAGGTCTTAGTCCTAAGAGGGAACAGTTTTTCCAACAGAAAACGGCCAGGAGCTCCAATGAGGTCAGAGTTGTTCACACTGAATGAATCAGATGACATAGTCAATTCAGTGAATGAATTCTACAGATCAGCGATATTGCCCGAGGTAACTAAGTTCCTGGACACAGAAAGCCCCCCATGGAAAGAATGGGTGGAGAAATTGGACAAGTACACAAGCATTCCGAATGAAGAATTGGATGAAGTTAGGAGTGCTTGGAAAGCATGGAAGTACAGGGAACATTGA

Protein sequence

MSLSFLKCLSIGNLIRSVKADRIHLSWLTERDASAGFYIFLLADLTERTAEKPGCQRGKERGAIEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPESAWIDDPCGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPAKLIGIESKLKTTEIDCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLVYALKSGKKVSPGDMSNSEFEGSDQENDTFVEGIKEPDQRSPSLSVCENLTEVCSKLEDGSELLKGVRARWLHEPDEKDRIGASHFRRILYCSCGKLETYFGCDFIEISIWGESFMLHQIRKMIATAVAVKRKLLPRDIITLSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPMRSELFTLNESDDIVNSVNEFYRSAILPEVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYREH*

Homology

[Close]

BLAST of CSPI01G24420 vs. ExPASy Swiss-Prot
Match: O22928 (Putative tRNA pseudouridine synthase OS=Arabidopsis thaliana OX=3702 GN=At2g30320 PE=3 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 1.6e-117
Identity = 214/404 (52.97%), Postives = 285/404 (70.54%), Query Frame = 0


Query: 64  IEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPESAWIDDP 123
           IE ELE AIYKAGGI DSNYG+L+KI WARSSRTDKGVHSLAT+I+LKMEIPE+AW DDP
Sbjct: 91  IEGELEVAIYKAGGIRDSNYGDLHKIGWARSSRTDKGVHSLATSISLKMEIPETAWKDDP 150

Query: 124 CGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPAKLIGIESKLKTTEI 183
            G  LA  I+ +LP+NI+VF ++P+ +RFDPR+EC +R YSYLLP  ++GI++   + EI
Sbjct: 151 QGTVLAKCISKHLPENIRVFSVLPSNRRFDPRRECTLRKYSYLLPVDVLGIKNSFTSDEI 210

Query: 184 DCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLVYALKSGKKVSPGDMSNSEFEG 243
           D HI+DF +ILK FEG++PFHNYT RS YR +   K     +K      P +  + +   
Sbjct: 211 DYHITDFNEILKEFEGEYPFHNYTQRSRYRRKSEQK-----IKQRNGRPPREPKSIKASE 270

Query: 244 SDQENDTFVEGIKEPDQRSPSLSVCENLTEVCSKLEDGSELLKGVRARWLHEPDEKDRIG 303
           S+   +  +E  +E +++       E+   V +   D S++    RA+WL+EPDE D+I 
Sbjct: 271 SEFREENHIEIGEEEEEKEVD---GESDEHVVTPDSDNSQVYS--RAKWLYEPDETDKIS 330

Query: 304 ASHFRRILYCSCGKLETYFGCDFIEISIWGESFMLHQIRKMIATAVAVKRKLLPRDIITL 363
            +HFR++  C  GKLE   G  F+EISIWGESFMLHQIRKMI TAVAVKR+LLPRDII L
Sbjct: 331 GAHFRKVFRCRSGKLENSLGFGFVEISIWGESFMLHQIRKMIGTAVAVKRELLPRDIIRL 390

Query: 364 SLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPMRSELFTLNESDDIVNSVNEFYRSAI 423
           SL KF+RIVLP+AP+EVL+LRGNSF  R+ P  P    +    ES+++   + EFYR+ +
Sbjct: 391 SLNKFTRIVLPLAPSEVLILRGNSFEVRRLPERP---GMEATGESEEVEKEIEEFYRAVM 450

Query: 424 LPEVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWK 468
           +P+V+ FLD+E  PWKEWV+ LD+   + +EEL++VR  W+ WK
Sbjct: 451 VPQVSIFLDSEKSPWKEWVDHLDRNDGLIDEELEDVRKGWEEWK 481

[Close]

BLAST of CSPI01G24420 vs. ExPASy Swiss-Prot
Match: Q59S63 (tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=PUS1 PE=3 SV=2)

HSP 1 Score: 99.4 bits (246), Expect = 1.2e-19
Identity = 102/343 (29.74%), Postives = 153/343 (44.61%), Query Frame = 0


Query: 64  IEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPESAWIDDP 123
           IE+++ DA+  AG I   N  +L K  + R++RTDKGVH+    I+LKM       I+DP
Sbjct: 176 IEKDIYDAMATAGAISAENAVDLKKSGFQRAARTDKGVHAAGNVISLKM------IIEDP 235

Query: 124 CGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPA-KLIGIESKLKTTE 183
               + + IN  LP  I+++GI    K FD RK C  R+Y YLLP   L+  + K   +E
Sbjct: 236 ---EIINKINDLLPKQIRIWGIQRTTKGFDCRKCCSSRIYEYLLPTFSLLPPKPKSVLSE 295

Query: 184 IDCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLVYALKSGKKVSPGDMSNSEFE 243
           +       K+  K  E    F +      +   +++K     L SG      D   S ++
Sbjct: 296 L------VKE--KKLENPDLFEDDQEGIDWWENVKSK----ILASGITQEQIDSITSSYD 355

Query: 244 GSDQENDTFVEGIKEPDQRSPSLSVCENLTEVCSKLEDGSELLKGVRARWLHEPDE--KD 303
              Q+     +   + ++R  S      L +    +E+ S     + +  L    E  K 
Sbjct: 356 EQQQQQQQQQQQQADEEERDISELSFTKLIKQIKTIENQSRRSYRISSSRLQHFREVMKQ 415

Query: 304 RIGASHFRRILYCSCGK--------LETYF-------GCDFIEISIWGESFMLHQIRKMI 363
             G  +F         K        ++T         G ++I I I G+SFMLHQIRKMI
Sbjct: 416 YEGTHNFHNFTVGKPFKDTSANRFMIKTIVSDPFVIEGTEWISIKIHGQSFMLHQIRKMI 475

Query: 364 ATAVAVKRKLLPRDIITLSLTKFSRIVLPIAPAEVLVLRGNSF 389
           A A  V R  LP  II  +  + ++I +P APA  L+L    F
Sbjct: 476 AMAALVVRLSLPCGIIN-NFFQSTKINIPKAPALGLLLENPVF 496

[Close]

BLAST of CSPI01G24420 vs. ExPASy Swiss-Prot
Match: Q6CC39 (tRNA pseudouridine synthase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=PUS1 PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.7e-18
Identity = 98/386 (25.39%), Postives = 174/386 (45.08%), Query Frame = 0


Query: 54  GCQRGKERGAIEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITLKME 113
           G Q    +  IE ++  A  KAG I  +N  +  K ++ R++RTDKGVH+    I+LKM 
Sbjct: 100 GMQLNPPQKTIEGDIFQAFVKAGAISQNNADDPKKSAFMRAARTDKGVHAAGNVISLKMI 159

Query: 114 IPESAWIDDPCGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPA---- 173
           I +          ++ + INS+LP+ ++V+G+    K F+ RK C  R+Y YL+P     
Sbjct: 160 IEDE---------NIVEKINSHLPEQLRVWGVSRTNKAFECRKLCSSRVYEYLMPTYSFL 219

Query: 174 ----KLIGIESKLK--TTEIDCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLVY 233
                 +  E  LK  T+  +     ++ +    E     ++  ++      I+ ++   
Sbjct: 220 NPRPGTVMSEKLLKDGTSPDEEGKKYWESVAADLESQGVSYDEWMKRACIDEIKGEE--- 279

Query: 234 ALKSGKKVSPGDMSNSEFEGSDQENDTFVEGIKEPDQRSPSLSVCENLTEVCSKLEDGSE 293
                      +++ SE +   + +   +E IK  ++R           E  +K+ +  +
Sbjct: 280 ---------TKEVAESEVKTDSKTDAATLEKIKAVERRHREEFRISG--ERLAKIREILK 339

Query: 294 LLKGVRARWLHEPDEKDRIGASHFRRILYCSCGKLETYFGCDFIEISIWGESFMLHQIRK 353
           + +G    + +    K     S  R +   +C       G +++ I I G+SFMLHQIRK
Sbjct: 340 IYEGTH-NFHNFTLGKAFKDPSAMRTMKSLTCSDPFLIDGTEWVSIKIHGQSFMLHQIRK 399

Query: 354 MIA-TAVAVKRKLLPRDIITLSLTKFSRIVLPIAPAEVLVLRGNSFS--NRKRPGAPMRS 413
           MI+  A++V+    P+ +I  +  K +RI +P APA  L+L    +   N+K  G   R 
Sbjct: 400 MISMVALSVRCNADPQKLIPQTFEK-ARINIPKAPALGLLLERPVYDSYNKKLQGEFGRE 459

Query: 414 ELFTLNESDDIVNSVNEFYRSAILPE 427
            +   N +D I    ++F    I  E
Sbjct: 460 GVHFDNWNDQIEAFKHKFIYDKIYAE 460

[Close]

BLAST of CSPI01G24420 vs. ExPASy Swiss-Prot
Match: Q6CWQ8 (tRNA pseudouridine synthase 1 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=PUS1 PE=3 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 2.9e-18
Identity = 95/343 (27.70%), Postives = 157/343 (45.77%), Query Frame = 0


Query: 54  GCQRGKERGAIEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITLKME 113
           G Q       IE+EL +A  +AG I  +N  +L K  + R++RTDKGVH+    I+LK+ 
Sbjct: 114 GMQYNPPNDTIEKELFEAFVRAGAISKANSTDLKKNGFQRAARTDKGVHAGGNVISLKL- 173

Query: 114 IPESAWIDDPCGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPA-KLI 173
                 I+DP    + + IN+ LPD I+V+ I    K FD RK C  R Y YLLP   LI
Sbjct: 174 -----IIEDP---EVKEKINNELPDQIRVWDISRVNKAFDCRKMCSSRWYEYLLPTYSLI 233

Query: 174 GIE-SKLKTTEIDCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLVYALKSGKKV 233
           G + S     EI+    +  ++++       F        + T   +K   +  +  +++
Sbjct: 234 GPKPSTYLYNEIEASKKEIPNVIQDDVESAQFW-----EAFSTEAESK---FTKEELEEI 293

Query: 234 SPGDMSNSEFEGSDQENDTFVEG-----IKEPDQRSPSLSV--CENLTEVCSKLEDGSEL 293
           +   M    F   D+ N+T+ +      ++   +R+  +S    +       + E     
Sbjct: 294 AAFVMPKDSF---DENNETYQKSKAFKKLEAEHKRAYRISKERLDRFRSALKQYEGTFNF 353

Query: 294 LKGVRARWLHEPDEKDRIGASHFRRILYCS----CGKLETYFGCDFIEISIWGESFMLHQ 353
                 +  ++P       A  F + +  S     G+ +T    +++ I I G+SFMLHQ
Sbjct: 354 HNFTLGKDFNDP------SAKRFMKQITVSEPFVIGEAKT----EWVSIKIHGQSFMLHQ 413

Query: 354 IRKMIATAVAVKRKLLPRDIITLSLTKFSRIVLPIAPAEVLVL 384
           IRKMI+ A  + R   P + I  +  +  +I +P APA  L+L
Sbjct: 414 IRKMISMATLITRCFSPLERIRQAYGQ-EKINIPKAPALGLLL 425

[Close]

BLAST of CSPI01G24420 vs. ExPASy Swiss-Prot
Match: P53167 (tRNA pseudouridine(27/28) synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PUS2 PE=1 SV=4)

HSP 1 Score: 89.4 bits (220), Expect = 1.2e-16
Identity = 97/383 (25.33%), Postives = 149/383 (38.90%), Query Frame = 0


Query: 54  GCQRGKERGAIEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITLKME 113
           G Q       IE E+   ++  G I + N     K S+  ++RTDKGVH++   ++LK+ 
Sbjct: 12  GMQYNPPHKTIEGEILTKLFDVGAISEENSLAPKKNSFMAAARTDKGVHAMLNLLSLKIT 71

Query: 114 IPESAWIDDPCGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPAKLIG 173
           + E               +N+ LP  I+V+GI P  K+F+ R  CD R Y YL+P  ++ 
Sbjct: 72  LREDT----------VAKLNAALPPEIRVWGIQPVNKKFNARSACDSRWYQYLIPEFIL- 131

Query: 174 IESKLKTTEIDCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLVYALKSGKKVSP 233
                        I   +  L          +  V   YR                    
Sbjct: 132 -------------IGPPRSSLL---------HRNVGGCYRE------------------- 191

Query: 234 GDMSNSEFEGSDQENDTFVE---GIKEPDQRSPSLSVCENLTEVCSKLEDGSELLKGVRA 293
                   +GS +  DTF+E   G    D+        + L+E    ++D   LL G  +
Sbjct: 192 --------DGSQEVWDTFLEQTRGRFSGDELCRLQDTAQKLSESDPLVQDYVGLLSGTLS 251

Query: 294 RWLHEPDEKDR--------IGASHFRRILYCSCGKL--------------ETYFGCDFIE 353
            +   P + D         +G  +F      + GKL               +     +I 
Sbjct: 252 GYCLSPSKLDAFEAAMQEYVGTHNFHNF---TTGKLWGDPSAQRHIKKVVVSQASPGWIC 311

Query: 354 ISIWGESFMLHQIRKMIATAVAVKRKLLPRDIITLSLTKFSRIVLPIAPAEVLVLRGNSF 412
           + I G+SFMLHQIR+M+A AV   R  LP +I+        R  +P APA+ L+L G  F
Sbjct: 312 VRIHGQSFMLHQIRRMVALAVLAARCQLPPNIVRNYFNAGPRKYIPRAPAQGLLLEGPVF 328

[Close]

BLAST of CSPI01G24420 vs. ExPASy TrEMBL
Match: A0A0A0M0Z2 (PseudoU_synth_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G533680 PE=3 SV=1)

HSP 1 Score: 830.5 bits (2144), Expect = 3.6e-237
Identity = 408/409 (99.76%), Postives = 408/409 (99.76%), Query Frame = 0


Query: 63  AIEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPESAWIDD 122
           AIEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITLKMEIP SAWIDD
Sbjct: 13  AIEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPGSAWIDD 72

Query: 123 PCGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPAKLIGIESKLKTTE 182
           PCGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPAKLIGIESKLKTTE
Sbjct: 73  PCGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPAKLIGIESKLKTTE 132

Query: 183 IDCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLVYALKSGKKVSPGDMSNSEFE 242
           IDCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLVYALKSGKKVSPGDMSNSEFE
Sbjct: 133 IDCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLVYALKSGKKVSPGDMSNSEFE 192

Query: 243 GSDQENDTFVEGIKEPDQRSPSLSVCENLTEVCSKLEDGSELLKGVRARWLHEPDEKDRI 302
           GSDQENDTFVEGIKEPDQRSPSLSVCENLTEVCSKLEDGSELLKGVRARWLHEPDEKDRI
Sbjct: 193 GSDQENDTFVEGIKEPDQRSPSLSVCENLTEVCSKLEDGSELLKGVRARWLHEPDEKDRI 252

Query: 303 GASHFRRILYCSCGKLETYFGCDFIEISIWGESFMLHQIRKMIATAVAVKRKLLPRDIIT 362
           GASHFRRILYCSCGKLETYFGCDFIEISIWGESFMLHQIRKMIATAVAVKRKLLPRDIIT
Sbjct: 253 GASHFRRILYCSCGKLETYFGCDFIEISIWGESFMLHQIRKMIATAVAVKRKLLPRDIIT 312

Query: 363 LSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPMRSELFTLNESDDIVNSVNEFYRSA 422
           LSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPMRSELFTLNESDDIVNSVNEFYRSA
Sbjct: 313 LSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPMRSELFTLNESDDIVNSVNEFYRSA 372

Query: 423 ILPEVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYREH 472
           ILPEVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYREH
Sbjct: 373 ILPEVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYREH 421

[Close]

BLAST of CSPI01G24420 vs. ExPASy TrEMBL
Match: A0A1S3C5W1 (putative tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497029 PE=3 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 8.8e-212
Identity = 369/409 (90.22%), Postives = 383/409 (93.64%), Query Frame = 0


Query: 64  IEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPESAWIDDP 123
           IEQELE AIYKAGGI +SNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPESAW+ DP
Sbjct: 100 IEQELEAAIYKAGGIRESNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDP 159

Query: 124 CGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPAKLIGIESKLKTTEI 183
           CGISLADYINSYLPDNIKVFGIVP+Q+ FDPRKECDVR YSYLLPAKLIGIESKLKTTEI
Sbjct: 160 CGISLADYINSYLPDNIKVFGIVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEI 219

Query: 184 DCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLV-YALKSGKKVSPGDMSNSEFE 243
           DCHISDFKDIL SFEG+HPFHNYT+RS YRTRIRAKQ   YALKSG KVSPG+MSN EFE
Sbjct: 220 DCHISDFKDILNSFEGEHPFHNYTIRSNYRTRIRAKQSPGYALKSGNKVSPGEMSNFEFE 279

Query: 244 GSDQENDTFVEGIKEPDQRSPSLSVCENLTEVCSKLEDGSELLKGVRARWLHEPDEKDRI 303
            SD+E+DTFVEG+KEPDQ SPSLSVCENLTEVCSKLEDGSELLKGVRARWLHEPDEKDRI
Sbjct: 280 ESDREDDTFVEGVKEPDQCSPSLSVCENLTEVCSKLEDGSELLKGVRARWLHEPDEKDRI 339

Query: 304 GASHFRRILYCSCGKLETYFGCDFIEISIWGESFMLHQIRKMIATAVAVKRKLLPRDIIT 363
           GASHFRRIL CSCGKLET FG DFIEISIWGESFMLHQIRKMI TAVAVKRKLLPRD+I 
Sbjct: 340 GASHFRRILCCSCGKLETSFGYDFIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRDVIA 399

Query: 364 LSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPMRSELFTLNESDDIVNSVNEFYRSA 423
           LSLTKFSRIVLPIAPAEVLVLRGNSFS RKRPG P R +L TLNESDDI+ SVNEFYRSA
Sbjct: 400 LSLTKFSRIVLPIAPAEVLVLRGNSFSTRKRPGVPTRPDLVTLNESDDIIKSVNEFYRSA 459

Query: 424 ILPEVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYREH 472
           ILPEVT+FLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYR H
Sbjct: 460 ILPEVTEFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYRVH 508

[Close]

BLAST of CSPI01G24420 vs. ExPASy TrEMBL
Match: A0A6J1I156 (putative tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111469569 PE=3 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 2.9e-186
Identity = 334/419 (79.71%), Postives = 365/419 (87.11%), Query Frame = 0


Query: 54  GCQRGKERGA---IEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITL 113
           G Q  +E+ +   IEQELE AIYKAGGI +SNYGNLNKISWARSSRTDKGVHSLATTITL
Sbjct: 87  GLQMQREQSSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARSSRTDKGVHSLATTITL 146

Query: 114 KMEIPESAWIDDPCGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPAK 173
           KMEIP+SAW+ DPCGI LA+YIN YLP+NIKVFGIVP+Q+ FDPR+ECDVR Y+YLLPAK
Sbjct: 147 KMEIPDSAWMGDPCGILLANYINFYLPENIKVFGIVPSQRSFDPRRECDVRKYTYLLPAK 206

Query: 174 LIGIESKLKTTEIDCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLV-YALKSGK 233
           LIGIESKL TTEID HIS+FKDIL SF+G+HPFHNYT+RS YRTRIRA Q   Y  KSG 
Sbjct: 207 LIGIESKLTTTEIDYHISEFKDILNSFQGEHPFHNYTIRSKYRTRIRANQSPGYVHKSGD 266

Query: 234 KVSPGDMSNSEFEGSDQENDTFV-EGIKEPDQRSPSLSVC-ENLTEVCSKLEDGSELLKG 293
            VS  D+SNSEFE SD END FV  GIKEP+Q SPSLS C EN  EVCSKL +GSEL +G
Sbjct: 267 GVSSSDLSNSEFEESDGENDIFVGGGIKEPNQHSPSLSGCNENTFEVCSKLGNGSELPQG 326

Query: 294 VRARWLHEPDEKDRIGASHFRRILYCSCGKLETYFGCDFIEISIWGESFMLHQIRKMIAT 353
           VRARWLHEPDE+DRIGASHFR+IL CSCGKLET  G DF+EISIWGESFMLHQIRKM+ T
Sbjct: 327 VRARWLHEPDERDRIGASHFRKILCCSCGKLETSLGYDFVEISIWGESFMLHQIRKMVGT 386

Query: 354 AVAVKRKLLPRDIITLSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPMRSELFTLNE 413
           A+AVKRKLLPRDII LSL+KFSRIVLPIAPAEVLVLRGN FSNRKRPGAP R +L TLN+
Sbjct: 387 AIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLVLRGNRFSNRKRPGAPKRPDLLTLNK 446

Query: 414 SDDIVNSVNEFYRSAILPEVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAW 467
           SDDIV SVNEFYRSAILPEVT FLD E PPWKEWVEKLDKYTSIPNE+L+EVR+AWKAW
Sbjct: 447 SDDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWVEKLDKYTSIPNEQLEEVRNAWKAW 505

[Close]

BLAST of CSPI01G24420 vs. ExPASy TrEMBL
Match: A0A6J1HM83 (putative tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111464888 PE=3 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 3.7e-186
Identity = 332/418 (79.43%), Postives = 363/418 (86.84%), Query Frame = 0


Query: 54  GCQRGKERGA---IEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITL 113
           G Q  +E+ +   IEQELE AIYKAGGI +SNYG+LNKISWARSSRTDKGVHSLATTITL
Sbjct: 87  GLQMQREQSSHPTIEQELEAAIYKAGGIRESNYGDLNKISWARSSRTDKGVHSLATTITL 146

Query: 114 KMEIPESAWIDDPCGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPAK 173
           KMEIP+SAW+ DPCGI LA+YIN YLP+NI+VFGIVP+Q+ FDPR+ECDVR Y+YLLPAK
Sbjct: 147 KMEIPDSAWMGDPCGILLANYINFYLPENIRVFGIVPSQRSFDPRRECDVRKYTYLLPAK 206

Query: 174 LIGIESKLKTTEIDCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLV-YALKSGK 233
           LIGIESKL TTEID HIS+FKDIL SF+G+HPFHNYT+RS YRTRIR  Q   Y  KSG 
Sbjct: 207 LIGIESKLTTTEIDYHISEFKDILNSFQGEHPFHNYTIRSKYRTRIRTNQSPGYVHKSGD 266

Query: 234 KVSPGDMSNSEFEGSDQENDTFVEGIKEPDQRSPSLS-VCENLTEVCSKLEDGSELLKGV 293
            VS  D+SNSEFE SD END FV GIKEP+Q SPSLS   EN  EVCSKL +GSEL +GV
Sbjct: 267 GVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSPSLSGSYENTFEVCSKLGNGSELPQGV 326

Query: 294 RARWLHEPDEKDRIGASHFRRILYCSCGKLETYFGCDFIEISIWGESFMLHQIRKMIATA 353
           RARWLHEPDEKDRIGASHFR+ILYCSCGKLET  G DF+EISIWGESFMLHQIRKM+ TA
Sbjct: 327 RARWLHEPDEKDRIGASHFRKILYCSCGKLETSLGYDFVEISIWGESFMLHQIRKMVGTA 386

Query: 354 VAVKRKLLPRDIITLSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPMRSELFTLNES 413
           +AVKRKLLPRDII LSL+KFSRIVLPIAPAEVLVLRGN FSNRKRPGAP R +L TLNES
Sbjct: 387 IAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLVLRGNRFSNRKRPGAPKRPDLLTLNES 446

Query: 414 DDIVNSVNEFYRSAILPEVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAW 467
            DIV SVNEFYRSAILPEVT FLD E PPWKEWVEKLDKYTSIPNE+L+EVR+AWKAW
Sbjct: 447 HDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWVEKLDKYTSIPNEQLEEVRNAWKAW 504

[Close]

BLAST of CSPI01G24420 vs. ExPASy TrEMBL
Match: A0A6J1DMV6 (putative tRNA pseudouridine synthase isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022025 PE=3 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 1.3e-183
Identity = 324/406 (79.80%), Postives = 356/406 (87.68%), Query Frame = 0


Query: 64  IEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPESAWIDDP 123
           IEQELE AI+KAGGI DSNYGNL KISWARSSRTDKGVHSLATTI+LKMEIPE+AW+ DP
Sbjct: 99  IEQELEAAIFKAGGIRDSNYGNLYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDP 158

Query: 124 CGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPAKLIGIESKLKTTEI 183
           CGI LA+YIN YLPDNIKVFGIVP+Q+ FDPR+ECDVR YSYLLPAKLIGIESK  TTEI
Sbjct: 159 CGILLANYINFYLPDNIKVFGIVPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEI 218

Query: 184 DCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLV-YALKSGKKVSPGDMSNSEFE 243
           D HIS+FKDIL SF+G+HPFHNYT+RS YR  IRA+Q   Y   SG++VSP  MSNS FE
Sbjct: 219 DHHISEFKDILNSFQGEHPFHNYTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFE 278

Query: 244 GSDQENDTFVEGIKEPDQRSPSLSV-CENLTEVCSKLEDGSELLKGVRARWLHEPDEKDR 303
            SD E++ FVEG+K P+Q +P LSV CE+  EVCSKL +GSEL KGVRARWLHEPDEKDR
Sbjct: 279 ESDSEDNFFVEGVKGPNQHAPRLSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDR 338

Query: 304 IGASHFRRILYCSCGKLETYFGCDFIEISIWGESFMLHQIRKMIATAVAVKRKLLPRDII 363
           IGASHFR+IL CSCGKLET  G DF+EISIWGESFMLHQIRKM+ TAVAVKRKLLPRDII
Sbjct: 339 IGASHFRKILCCSCGKLETSLGYDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDII 398

Query: 364 TLSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPMRSELFTLNESDDIVNSVNEFYRS 423
            LSLTKFSRIVLPIAPAEVLVLRGNSFSN KRPGAP R +L TLN+SDDI+ SV+EFYRS
Sbjct: 399 ALSLTKFSRIVLPIAPAEVLVLRGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRS 458

Query: 424 AILPEVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWK 468
           AILPE+T FLDTE  PW EWVEKLDKYTSIPNE+LDEVR+AWKAWK
Sbjct: 459 AILPELTNFLDTEKAPWNEWVEKLDKYTSIPNEQLDEVRNAWKAWK 504

[Close]

BLAST of CSPI01G24420 vs. NCBI nr
Match: XP_031742713.1 (putative tRNA pseudouridine synthase isoform X1 [Cucumis sativus])

HSP 1 Score: 830.1 bits (2143), Expect = 9.6e-237
Identity = 408/411 (99.27%), Postives = 409/411 (99.51%), Query Frame = 0


Query: 61  RGAIEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPESAWI 120
           R +IEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITLKMEIP SAWI
Sbjct: 116 RFSIEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPGSAWI 175

Query: 121 DDPCGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPAKLIGIESKLKT 180
           DDPCGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPAKLIGIESKLKT
Sbjct: 176 DDPCGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPAKLIGIESKLKT 235

Query: 181 TEIDCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLVYALKSGKKVSPGDMSNSE 240
           TEIDCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLVYALKSGKKVSPGDMSNSE
Sbjct: 236 TEIDCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLVYALKSGKKVSPGDMSNSE 295

Query: 241 FEGSDQENDTFVEGIKEPDQRSPSLSVCENLTEVCSKLEDGSELLKGVRARWLHEPDEKD 300
           FEGSDQENDTFVEGIKEPDQRSPSLSVCENLTEVCSKLEDGSELLKGVRARWLHEPDEKD
Sbjct: 296 FEGSDQENDTFVEGIKEPDQRSPSLSVCENLTEVCSKLEDGSELLKGVRARWLHEPDEKD 355

Query: 301 RIGASHFRRILYCSCGKLETYFGCDFIEISIWGESFMLHQIRKMIATAVAVKRKLLPRDI 360
           RIGASHFRRILYCSCGKLETYFGCDFIEISIWGESFMLHQIRKMIATAVAVKRKLLPRDI
Sbjct: 356 RIGASHFRRILYCSCGKLETYFGCDFIEISIWGESFMLHQIRKMIATAVAVKRKLLPRDI 415

Query: 361 ITLSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPMRSELFTLNESDDIVNSVNEFYR 420
           ITLSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPMRSELFTLNESDDIVNSVNEFYR
Sbjct: 416 ITLSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPMRSELFTLNESDDIVNSVNEFYR 475

Query: 421 SAILPEVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYREH 472
           SAILPEVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYREH
Sbjct: 476 SAILPEVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYREH 526

[Close]

BLAST of CSPI01G24420 vs. NCBI nr
Match: XP_004145230.1 (putative tRNA pseudouridine synthase isoform X2 [Cucumis sativus] >KGN66289.2 hypothetical protein Csa_023335 [Cucumis sativus])

HSP 1 Score: 828.9 bits (2140), Expect = 2.1e-236
Identity = 407/408 (99.75%), Postives = 407/408 (99.75%), Query Frame = 0


Query: 64  IEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPESAWIDDP 123
           IEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITLKMEIP SAWIDDP
Sbjct: 100 IEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPGSAWIDDP 159

Query: 124 CGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPAKLIGIESKLKTTEI 183
           CGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPAKLIGIESKLKTTEI
Sbjct: 160 CGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPAKLIGIESKLKTTEI 219

Query: 184 DCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLVYALKSGKKVSPGDMSNSEFEG 243
           DCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLVYALKSGKKVSPGDMSNSEFEG
Sbjct: 220 DCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLVYALKSGKKVSPGDMSNSEFEG 279

Query: 244 SDQENDTFVEGIKEPDQRSPSLSVCENLTEVCSKLEDGSELLKGVRARWLHEPDEKDRIG 303
           SDQENDTFVEGIKEPDQRSPSLSVCENLTEVCSKLEDGSELLKGVRARWLHEPDEKDRIG
Sbjct: 280 SDQENDTFVEGIKEPDQRSPSLSVCENLTEVCSKLEDGSELLKGVRARWLHEPDEKDRIG 339

Query: 304 ASHFRRILYCSCGKLETYFGCDFIEISIWGESFMLHQIRKMIATAVAVKRKLLPRDIITL 363
           ASHFRRILYCSCGKLETYFGCDFIEISIWGESFMLHQIRKMIATAVAVKRKLLPRDIITL
Sbjct: 340 ASHFRRILYCSCGKLETYFGCDFIEISIWGESFMLHQIRKMIATAVAVKRKLLPRDIITL 399

Query: 364 SLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPMRSELFTLNESDDIVNSVNEFYRSAI 423
           SLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPMRSELFTLNESDDIVNSVNEFYRSAI
Sbjct: 400 SLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPMRSELFTLNESDDIVNSVNEFYRSAI 459

Query: 424 LPEVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYREH 472
           LPEVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYREH
Sbjct: 460 LPEVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYREH 507

[Close]

BLAST of CSPI01G24420 vs. NCBI nr
Match: XP_008457308.1 (PREDICTED: putative tRNA pseudouridine synthase [Cucumis melo])

HSP 1 Score: 746.1 bits (1925), Expect = 1.8e-211
Identity = 369/409 (90.22%), Postives = 383/409 (93.64%), Query Frame = 0


Query: 64  IEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPESAWIDDP 123
           IEQELE AIYKAGGI +SNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPESAW+ DP
Sbjct: 100 IEQELEAAIYKAGGIRESNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDP 159

Query: 124 CGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPAKLIGIESKLKTTEI 183
           CGISLADYINSYLPDNIKVFGIVP+Q+ FDPRKECDVR YSYLLPAKLIGIESKLKTTEI
Sbjct: 160 CGISLADYINSYLPDNIKVFGIVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEI 219

Query: 184 DCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLV-YALKSGKKVSPGDMSNSEFE 243
           DCHISDFKDIL SFEG+HPFHNYT+RS YRTRIRAKQ   YALKSG KVSPG+MSN EFE
Sbjct: 220 DCHISDFKDILNSFEGEHPFHNYTIRSNYRTRIRAKQSPGYALKSGNKVSPGEMSNFEFE 279

Query: 244 GSDQENDTFVEGIKEPDQRSPSLSVCENLTEVCSKLEDGSELLKGVRARWLHEPDEKDRI 303
            SD+E+DTFVEG+KEPDQ SPSLSVCENLTEVCSKLEDGSELLKGVRARWLHEPDEKDRI
Sbjct: 280 ESDREDDTFVEGVKEPDQCSPSLSVCENLTEVCSKLEDGSELLKGVRARWLHEPDEKDRI 339

Query: 304 GASHFRRILYCSCGKLETYFGCDFIEISIWGESFMLHQIRKMIATAVAVKRKLLPRDIIT 363
           GASHFRRIL CSCGKLET FG DFIEISIWGESFMLHQIRKMI TAVAVKRKLLPRD+I 
Sbjct: 340 GASHFRRILCCSCGKLETSFGYDFIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRDVIA 399

Query: 364 LSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPMRSELFTLNESDDIVNSVNEFYRSA 423
           LSLTKFSRIVLPIAPAEVLVLRGNSFS RKRPG P R +L TLNESDDI+ SVNEFYRSA
Sbjct: 400 LSLTKFSRIVLPIAPAEVLVLRGNSFSTRKRPGVPTRPDLVTLNESDDIIKSVNEFYRSA 459

Query: 424 ILPEVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYREH 472
           ILPEVT+FLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYR H
Sbjct: 460 ILPEVTEFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYRVH 508

[Close]

BLAST of CSPI01G24420 vs. NCBI nr
Match: XP_038894775.1 (putative tRNA pseudouridine synthase isoform X2 [Benincasa hispida])

HSP 1 Score: 692.6 bits (1786), Expect = 2.4e-195
Identity = 344/413 (83.29%), Postives = 371/413 (89.83%), Query Frame = 0


Query: 60  ERGAIEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPESAW 119
           +R +IEQELE AIYKAGGI +SNYGNLNKISWARSSRTDKGVHSLATTI LKMEIPESAW
Sbjct: 13  KRTSIEQELEAAIYKAGGIRESNYGNLNKISWARSSRTDKGVHSLATTIALKMEIPESAW 72

Query: 120 IDDPCGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPAKLIGIESKLK 179
           + DPCGISLA+YIN YLPDNIKVFGIVP+QK FDPRKECDVR YSY+LPAKLIGI +KLK
Sbjct: 73  MGDPCGISLANYINFYLPDNIKVFGIVPSQKSFDPRKECDVRKYSYVLPAKLIGIGNKLK 132

Query: 180 TTEIDCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLVYALKSGKKVSPGDMSNS 239
           TTE+D HISDFKDIL SF+G+HPFHNYT+RS YRTRIRAK+   +   G +VSP D+SNS
Sbjct: 133 TTEVDYHISDFKDILNSFQGEHPFHNYTIRSKYRTRIRAKR---SPGYGHEVSPSDVSNS 192

Query: 240 EFEGSDQENDTFVEGIKEPDQRSPSLSVC-ENLTEVCSKLEDGSELLKGVRARWLHEPDE 299
           EFE SD E+DTFVEG+KEPDQ SPSLSVC EN TEVCSKL DGSEL K VRARWLHEPDE
Sbjct: 193 EFEESDGEDDTFVEGVKEPDQGSPSLSVCYENPTEVCSKLGDGSELPKDVRARWLHEPDE 252

Query: 300 KDRIGASHFRRILYCSCGKLETYFGCDFIEISIWGESFMLHQIRKMIATAVAVKRKLLPR 359
           KDRIGASHFR+IL CSCGKLET FG DF+EISIWGESFMLHQIRKMI TA+AVKRKLLPR
Sbjct: 253 KDRIGASHFRKILCCSCGKLETSFGYDFVEISIWGESFMLHQIRKMIGTAIAVKRKLLPR 312

Query: 360 DIITLSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPMRSELFTLNESDDIVNSVNEF 419
           D+I LSL+KFSRIVLPIAPAEVLVLRGNSFSNRKRPG P R +L TLNESDDIV SVNEF
Sbjct: 313 DVIALSLSKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEF 372

Query: 420 YRSAILPEVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYREH 472
           YRSAILPEVT FLDTE PPWKEWVEKLDKYTSIP+E+LDEVR+AWKAW YR H
Sbjct: 373 YRSAILPEVTNFLDTERPPWKEWVEKLDKYTSIPSEQLDEVRNAWKAWNYRVH 422

[Close]

BLAST of CSPI01G24420 vs. NCBI nr
Match: XP_038894774.1 (putative tRNA pseudouridine synthase isoform X1 [Benincasa hispida])

HSP 1 Score: 691.0 bits (1782), Expect = 7.0e-195
Identity = 343/409 (83.86%), Postives = 368/409 (89.98%), Query Frame = 0


Query: 64  IEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPESAWIDDP 123
           IEQELE AIYKAGGI +SNYGNLNKISWARSSRTDKGVHSLATTI LKMEIPESAW+ DP
Sbjct: 100 IEQELEAAIYKAGGIRESNYGNLNKISWARSSRTDKGVHSLATTIALKMEIPESAWMGDP 159

Query: 124 CGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPAKLIGIESKLKTTEI 183
           CGISLA+YIN YLPDNIKVFGIVP+QK FDPRKECDVR YSY+LPAKLIGI +KLKTTE+
Sbjct: 160 CGISLANYINFYLPDNIKVFGIVPSQKSFDPRKECDVRKYSYVLPAKLIGIGNKLKTTEV 219

Query: 184 DCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLVYALKSGKKVSPGDMSNSEFEG 243
           D HISDFKDIL SF+G+HPFHNYT+RS YRTRIRAK+   +   G +VSP D+SNSEFE 
Sbjct: 220 DYHISDFKDILNSFQGEHPFHNYTIRSKYRTRIRAKR---SPGYGHEVSPSDVSNSEFEE 279

Query: 244 SDQENDTFVEGIKEPDQRSPSLSVC-ENLTEVCSKLEDGSELLKGVRARWLHEPDEKDRI 303
           SD E+DTFVEG+KEPDQ SPSLSVC EN TEVCSKL DGSEL K VRARWLHEPDEKDRI
Sbjct: 280 SDGEDDTFVEGVKEPDQGSPSLSVCYENPTEVCSKLGDGSELPKDVRARWLHEPDEKDRI 339

Query: 304 GASHFRRILYCSCGKLETYFGCDFIEISIWGESFMLHQIRKMIATAVAVKRKLLPRDIIT 363
           GASHFR+IL CSCGKLET FG DF+EISIWGESFMLHQIRKMI TA+AVKRKLLPRD+I 
Sbjct: 340 GASHFRKILCCSCGKLETSFGYDFVEISIWGESFMLHQIRKMIGTAIAVKRKLLPRDVIA 399

Query: 364 LSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPMRSELFTLNESDDIVNSVNEFYRSA 423
           LSL+KFSRIVLPIAPAEVLVLRGNSFSNRKRPG P R +L TLNESDDIV SVNEFYRSA
Sbjct: 400 LSLSKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSA 459

Query: 424 ILPEVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYREH 472
           ILPEVT FLDTE PPWKEWVEKLDKYTSIP+E+LDEVR+AWKAW YR H
Sbjct: 460 ILPEVTNFLDTERPPWKEWVEKLDKYTSIPSEQLDEVRNAWKAWNYRVH 505

[Close]

BLAST of CSPI01G24420 vs. TAIR 10
Match: AT2G30320.1 (Pseudouridine synthase family protein )

HSP 1 Score: 424.5 bits (1090), Expect = 1.1e-118
Identity = 214/404 (52.97%), Postives = 285/404 (70.54%), Query Frame = 0


Query: 64  IEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPESAWIDDP 123
           IE ELE AIYKAGGI DSNYG+L+KI WARSSRTDKGVHSLAT+I+LKMEIPE+AW DDP
Sbjct: 91  IEGELEVAIYKAGGIRDSNYGDLHKIGWARSSRTDKGVHSLATSISLKMEIPETAWKDDP 150

Query: 124 CGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPAKLIGIESKLKTTEI 183
            G  LA  I+ +LP+NI+VF ++P+ +RFDPR+EC +R YSYLLP  ++GI++   + EI
Sbjct: 151 QGTVLAKCISKHLPENIRVFSVLPSNRRFDPRRECTLRKYSYLLPVDVLGIKNSFTSDEI 210

Query: 184 DCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLVYALKSGKKVSPGDMSNSEFEG 243
           D HI+DF +ILK FEG++PFHNYT RS YR +   K     +K      P +  + +   
Sbjct: 211 DYHITDFNEILKEFEGEYPFHNYTQRSRYRRKSEQK-----IKQRNGRPPREPKSIKASE 270

Query: 244 SDQENDTFVEGIKEPDQRSPSLSVCENLTEVCSKLEDGSELLKGVRARWLHEPDEKDRIG 303
           S+   +  +E  +E +++       E+   V +   D S++    RA+WL+EPDE D+I 
Sbjct: 271 SEFREENHIEIGEEEEEKEVD---GESDEHVVTPDSDNSQVYS--RAKWLYEPDETDKIS 330

Query: 304 ASHFRRILYCSCGKLETYFGCDFIEISIWGESFMLHQIRKMIATAVAVKRKLLPRDIITL 363
            +HFR++  C  GKLE   G  F+EISIWGESFMLHQIRKMI TAVAVKR+LLPRDII L
Sbjct: 331 GAHFRKVFRCRSGKLENSLGFGFVEISIWGESFMLHQIRKMIGTAVAVKRELLPRDIIRL 390

Query: 364 SLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPMRSELFTLNESDDIVNSVNEFYRSAI 423
           SL KF+RIVLP+AP+EVL+LRGNSF  R+ P  P    +    ES+++   + EFYR+ +
Sbjct: 391 SLNKFTRIVLPLAPSEVLILRGNSFEVRRLPERP---GMEATGESEEVEKEIEEFYRAVM 450

Query: 424 LPEVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWK 468
           +P+V+ FLD+E  PWKEWV+ LD+   + +EEL++VR  W+ WK
Sbjct: 451 VPQVSIFLDSEKSPWKEWVDHLDRNDGLIDEELEDVRKGWEEWK 481

[Close]

BLAST of CSPI01G24420 vs. TAIR 10
Match: AT1G20370.1 (Pseudouridine synthase family protein )

HSP 1 Score: 109.8 bits (273), Expect = 6.2e-24
Identity = 98/377 (25.99%), Postives = 161/377 (42.71%), Query Frame = 0


Query: 54  GCQRGKERGAIEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITLKME 113
           G Q+      IE ELE+A++ AG + D++        WARS+RTDKGV ++   ++ +  
Sbjct: 73  GMQKNPGAKTIEGELEEALFHAGAVPDADRNKPRNYEWARSARTDKGVSAVGQVVSGRF- 132

Query: 114 IPESAWIDDPCGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPA---- 173
                ++D P      + +NS LPD I+VFG       F  +K CD R Y YL+P     
Sbjct: 133 -----YVDPP---GFVERLNSKLPDQIRVFGYKRVAPSFSSKKFCDRRRYVYLIPVFALD 192

Query: 174 KLIGIESKLKTTE--------IDCHISDFKDIL------------KSFEGDHPFHNYTVR 233
             +  E+++  T+        ++C    +K  L            KS E      + T  
Sbjct: 193 PCVHGEAEMVRTDLGYEYVKCVECSEKGYKIPLGVMGKDTDCCDTKSLEIQSDISSSTCD 252

Query: 234 SIYRTRIRAKQLVYALKSGKKVSPGDMSNSEFEGSDQENDTFVEGIKEPDQRSPSLSVCE 293
           +     I A      L S    +  ++++   +G+D         +     ++  +    
Sbjct: 253 A-----IGADVKCETLSSSVPSAENNLNSEVLDGAD---------VSALSSKAEGMEESN 312

Query: 294 NLTEVCSKLEDGSELLKGVRARWLHEPDEKDR--------IGASHF-------------- 353
            L +V     +G ++ K   +++ +   EK+R        +G+ +F              
Sbjct: 313 TLAKVEMNNGEGEDMTKS-ESKFCYGEKEKERFNRILSYYVGSHNFHNFTTRTKAADPAA 372

Query: 354 -RRILYCSCGKLETYFGCDFIEISIWGESFMLHQIRKMIATAVAVKRKLLPRDIITLSLT 384
            R IL  +   +    G DF++  + G+SFMLHQIRKMI  AVA+ R   P  +I  S  
Sbjct: 373 NRYILSFNANTVINLDGKDFVKCEVVGQSFMLHQIRKMIGLAVAIMRNYAPESLIEASFK 425

[Close]

BLAST of CSPI01G24420 vs. TAIR 10
Match: AT1G76120.1 (Pseudouridine synthase family protein )

HSP 1 Score: 105.9 bits (263), Expect = 8.9e-23
Identity = 97/370 (26.22%), Postives = 158/370 (42.70%), Query Frame = 0


Query: 54  GCQRGKERGAIEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITLKME 113
           G Q+      IE ELE+A++ AG + +S  G      +ARS+RTDKGV ++        +
Sbjct: 37  GMQKNPGAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSARTDKGVSAVG-------Q 96

Query: 114 IPESAWIDDPCGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPAKLIG 173
           +    +I DP G    + +NS LP+ I++FG       F  +K CD R Y YLLP   + 
Sbjct: 97  VVSGRFIVDPLG--FVNRLNSNLPNQIRIFGYKHVTPSFSSKKFCDRRRYVYLLPVFALD 156

Query: 174 IESKLKTTEIDCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLVYALKSGKKVSP 233
             S      +   +   ++ +K FE                  R +++   L    K + 
Sbjct: 157 PISHRDRETVMASLGPGEEYVKCFECSE---------------RGRKIPPGLVGKWKGTN 216

Query: 234 GDMSNSEFEGSDQENDTFV--EGIKEPDQRSPSLSVCENLTEVCSKLEDGSELLKG---- 293
               + +F+     N++      IK     S    +C    EV    ED  +L       
Sbjct: 217 FGTKSLDFQSDISSNNSSALRSDIKIEALSSNLAGLCSVDVEVGRIQEDSCKLNTNSSET 276

Query: 294 -VRARWLHEPDEKDR--------IGASHF---------------RRILYCSCGKLETYFG 353
            V++++ +   EK+R        +G+ +F               R+I+  +   +    G
Sbjct: 277 KVKSKFCYGEKEKERFSRILSCYVGSYNFHNFTTRTKADDPTANRQIISFTANTVINLDG 336

Query: 354 CDFIEISIWGESFMLHQIRKMIATAVAVKRKLLPRDIITLSLTKFSRIVLPIAPAEVLVL 394
            DFI+  + G+SFMLHQIRKM+  AVA+ R      +I  + +K   I +P+AP   L L
Sbjct: 337 IDFIKCEVLGKSFMLHQIRKMMGLAVAIMRNCASESLIQSAFSKDVNITVPMAPEVGLYL 382

[Close]

BLAST of CSPI01G24420 vs. TAIR 10
Match: AT1G76120.2 (Pseudouridine synthase family protein )

HSP 1 Score: 97.8 bits (242), Expect = 2.4e-20
Identity = 89/344 (25.87%), Postives = 146/344 (42.44%), Query Frame = 0


Query: 54  GCQRGKERGAIEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITLKME 113
           G Q+      IE ELE+A++ AG + +S  G      +ARS+RTDKGV ++        +
Sbjct: 37  GMQKNPGAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSARTDKGVSAVG-------Q 96

Query: 114 IPESAWIDDPCGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPAKLIG 173
           +    +I DP G    + +NS LP+ I++FG       F  +K CD R Y YLLP   + 
Sbjct: 97  VVSGRFIVDPLG--FVNRLNSNLPNQIRIFGYKHVTPSFSSKKFCDRRRYVYLLPVFALD 156

Query: 174 IESKLKTTEIDCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLVYALKSGKKVSP 233
             S      +   +   ++ +K FE                  R +++   L    K + 
Sbjct: 157 PISHRDRETVMASLGPGEEYVKCFECSE---------------RGRKIPPGLVGKWKGTN 216

Query: 234 GDMSNSEFEGSDQENDTFV--EGIKEPDQRSPSLSVCENLTEVCSKLEDGSELLKG---- 293
               + +F+     N++      IK     S    +C    EV    ED  +L       
Sbjct: 217 FGTKSLDFQSDISSNNSSALRSDIKIEALSSNLAGLCSVDVEVGRIQEDSCKLNTNSSET 276

Query: 294 -VRARWLHEPDEKDR--------IGASHF---------------RRILYCSCGKLETYFG 353
            V++++ +   EK+R        +G+ +F               R+I+  +   +    G
Sbjct: 277 KVKSKFCYGEKEKERFSRILSCYVGSYNFHNFTTRTKADDPTANRQIISFTANTVINLDG 336

Query: 354 CDFIEISIWGESFMLHQIRKMIATAVAVKRKLLPRDIITLSLTK 368
            DFI+  + G+SFMLHQIRKM+  AVA+ R      +I  + +K
Sbjct: 337 IDFIKCEVLGKSFMLHQIRKMMGLAVAIMRNCASESLIQSAFSK 356

[Close]

BLAST of CSPI01G24420 vs. TAIR 10
Match: AT1G34150.1 (Pseudouridine synthase family protein )

HSP 1 Score: 49.7 bits (117), Expect = 7.6e-06
Identity = 44/175 (25.14%), Postives = 68/175 (38.86%), Query Frame = 0


Query: 64  IEQELEDAIYKAGGICDSNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPESAWIDDP 123
           +E  +E  ++KA        G+     ++R  RTDKGV S    I L +     + +  P
Sbjct: 112 MEPSIESEVFKALERTRLLVGDKKDSCYSRCGRTDKGVSSTGQVIALFLR----SRLKSP 171

Query: 124 CGISLA------------DYI---NSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLP 183
            G S A            DY+   N  LPD+I+V G  PA   F  R  C  R Y Y   
Sbjct: 172 PGDSKAQVNGRTGERPEYDYVRVLNRALPDDIRVIGWSPAPIDFHARFSCYAREYKYFFW 231

Query: 184 AKLIGIESKLKTTEIDCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLVY 224
            + + + +            DF    K F G+H F N+    +       +++ +
Sbjct: 232 RQNLNLSA-----------MDFAG--KKFIGEHDFRNFCKMDVANVHCYTRRVTF 269

The following BLAST results are available for this feature:

BLAST of CSPI01G24420 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22928	1.6e-117	52.97	Putative tRNA pseudouridine synthase OS=Arabidopsis thaliana OX=3702 GN=At2g3032...	[more]
Q59S63	1.2e-19	29.74	tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876...	[more]
Q6CC39	1.7e-18	25.39	tRNA pseudouridine synthase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) O...	[more]
Q6CWQ8	2.9e-18	27.70	tRNA pseudouridine synthase 1 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 23...	[more]
P53167	1.2e-16	25.33	tRNA pseudouridine(27/28) synthase OS=Saccharomyces cerevisiae (strain ATCC 2045...	[more]

BLAST of CSPI01G24420 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0M0Z2	3.6e-237	99.76	PseudoU_synth_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G53...	[more]
A0A1S3C5W1	8.8e-212	90.22	putative tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497029 PE=...	[more]
A0A6J1I156	2.9e-186	79.71	putative tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111469569...	[more]
A0A6J1HM83	3.7e-186	79.43	putative tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC1114648...	[more]
A0A6J1DMV6	1.3e-183	79.80	putative tRNA pseudouridine synthase isoform X2 OS=Momordica charantia OX=3673 G...	[more]

BLAST of CSPI01G24420 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031742713.1	9.6e-237	99.27	putative tRNA pseudouridine synthase isoform X1 [Cucumis sativus]	[more]
XP_004145230.1	2.1e-236	99.75	putative tRNA pseudouridine synthase isoform X2 [Cucumis sativus] >KGN66289.2 hy...	[more]
XP_008457308.1	1.8e-211	90.22	PREDICTED: putative tRNA pseudouridine synthase [Cucumis melo]	[more]
XP_038894775.1	2.4e-195	83.29	putative tRNA pseudouridine synthase isoform X2 [Benincasa hispida]	[more]
XP_038894774.1	7.0e-195	83.86	putative tRNA pseudouridine synthase isoform X1 [Benincasa hispida]	[more]

BLAST of CSPI01G24420 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G30320.1	1.1e-118	52.97	Pseudouridine synthase family protein	[more]
AT1G20370.1	6.2e-24	25.99	Pseudouridine synthase family protein	[more]
AT1G76120.1	8.9e-23	26.22	Pseudouridine synthase family protein	[more]
AT1G76120.2	2.4e-20	25.87	Pseudouridine synthase family protein	[more]
AT1G34150.1	7.6e-06	25.14	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020097	Pseudouridine synthase I, TruA, alpha/beta domain	PFAM	PF01416	PseudoU_synth_1	coord: 290..383 e-value: 1.4E-6 score: 28.7
IPR020094	Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal	GENE3D	3.30.70.580		coord: 53..158 e-value: 4.4E-20 score: 73.9
IPR020095	Pseudouridine synthase I, TruA, C-terminal	GENE3D	3.30.70.660		coord: 159..455 e-value: 1.6E-33 score: 117.9
None	No IPR available	PIRSR	PIRSR001430-2	PIRSR001430-2	coord: 88..213 e-value: 8.4E-16 score: 56.1
None	No IPR available	PANTHER	PTHR11142:SF21	TRNA PSEUDOURIDINE SYNTHASE	coord: 56..466
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 56..466
IPR041708	Pseudouridine synthase PUS1/ PUS2-like	CDD	cd02568	PseudoU_synth_PUS1_PUS2	coord: 54..388 e-value: 1.94706E-69 score: 219.407
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 304..390
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 54..215

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G24420.1	CSPI01G24420.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0031119	tRNA pseudouridine synthesis
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding