CSPI01G24410 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G24410
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionPseudoU_synth_1 domain-containing protein
LocationChr1: 19700379 .. 19700878 (+)
RNA-Seq ExpressionCSPI01G24410
SyntenyCSPI01G24410
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAATTCCCGCCCCCCAAGAATAGGGTGCAATGTCAGTCTCTTCTTTGCGACTCCCACTTTCCCCATGGATAACCAAAGCTTCATCTTCATGTTTTCGTTTCCCCGACCCTGTTTTCGTTTCCCGGACTCCTCCTCTTAGGCCTGCACTGCTCCACTTTTTTTCTTCTACTGCCTCCACCGATGCTTTTTCCACTCGGTGCGCTCCGGATCAGCTCCGGAAATGGCAAGCTTTCCGTAAGAAAAAGGTCGTTTTGCGAATCGGCTATGTCGGGACTGATTATCGAGGTCCTCATTTCACGTTACTCTCTGTTTCTCTTGACCATCCTCTTGTGAATCCTTCACCATTTCTCATTAACTCACTAGTTTTGACAATTCAGGGCTACAAATGCAACGCAACCAACTGTCACATCCAAGTAAGTTTTTCTCTACATTATATCAGGAAAATTCCTATCGATGGATTGACCATGGCATCCCCTGAATATTCTGTTGCTGTGTTATGA

mRNA sequence

GAATTCCCGCCCCCCAAGAATAGGGTGCAATGTCAGTCTCTTCTTTGCGACTCCCACTTTCCCCATGGATAACCAAAGCTTCATCTTCATGTTTTCGTTTCCCCGACCCTGTTTTCGTTTCCCGGACTCCTCCTCTTAGGCCTGCACTGCTCCACTTTTTTTCTTCTACTGCCTCCACCGATGCTTTTTCCACTCGGTGCGCTCCGGATCAGCTCCGGAAATGGCAAGCTTTCCGTAAGAAAAAGGTCGTTTTGCGAATCGGCTATGTCGGGACTGATTATCGAGGTCCTCATTTCACGTTACTCTCTGTTTCTCTTGACCATCCTCTTGTGAATCCTTCACCATTTCTCATTAACTCACTAGTTTTGACAATTCAGGGCTACAAATGCAACGCAACCAACTGTCACATCCAAGTAAGTTTTTCTCTACATTATATCAGGAAAATTCCTATCGATGGATTGACCATGGCATCCCCTGAATATTCTGTTGCTGTGTTATGA

Coding sequence (CDS)

ATGTCAGTCTCTTCTTTGCGACTCCCACTTTCCCCATGGATAACCAAAGCTTCATCTTCATGTTTTCGTTTCCCCGACCCTGTTTTCGTTTCCCGGACTCCTCCTCTTAGGCCTGCACTGCTCCACTTTTTTTCTTCTACTGCCTCCACCGATGCTTTTTCCACTCGGTGCGCTCCGGATCAGCTCCGGAAATGGCAAGCTTTCCGTAAGAAAAAGGTCGTTTTGCGAATCGGCTATGTCGGGACTGATTATCGAGGTCCTCATTTCACGTTACTCTCTGTTTCTCTTGACCATCCTCTTGTGAATCCTTCACCATTTCTCATTAACTCACTAGTTTTGACAATTCAGGGCTACAAATGCAACGCAACCAACTGTCACATCCAAGTAAGTTTTTCTCTACATTATATCAGGAAAATTCCTATCGATGGATTGACCATGGCATCCCCTGAATATTCTGTTGCTGTGTTATGA

Protein sequence

MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSTASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSLDHPLVNPSPFLINSLVLTIQGYKCNATNCHIQVSFSLHYIRKIPIDGLTMASPEYSVAVL*
Homology
[Close]
BLAST of CSPI01G24410 vs. ExPASy Swiss-Prot
Match: O22928 (Putative tRNA pseudouridine synthase OS=Arabidopsis thaliana OX=3702 GN=At2g30320 PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 2.0e-08
Identity = 41/105 (39.05%), Postives = 56/105 (53.33%), Query Frame = 0 

Query: 1   MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSTASTDAFSTRCAPD 60
           M+VS LR+P+        S  F +P     +R   + PALL F SS A   + ++   P 
Sbjct: 1   MAVSFLRIPI-------PSRVFWYP-----TRLIRVTPALLRFSSSAAFQPSSTSLSPPS 60

Query: 61  Q----LRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSLDHPLV 102
                  KW+++RKKKVV+RIGYVGTDYRG     L +  D P +
Sbjct: 61  SDNFLADKWESYRKKKVVIRIGYVGTDYRG-----LQIQRDDPSI 88

[Close]
BLAST of CSPI01G24410 vs. ExPASy TrEMBL
Match: A0A0A0LVB6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G533670 PE=4 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 3.4e-83
Identity = 156/157 (99.36%), Postives = 156/157 (99.36%), Query Frame = 0 

Query: 1   MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFF-SSTASTDAFSTRCAP 60
           MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFF SSTASTDAFSTRCAP
Sbjct: 1   MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSSTASTDAFSTRCAP 60

Query: 61  DQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSLDHPLVNPSPFLINSLVLTIQGYK 120
           DQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSLDHPLVNPSPFLINSLVLTIQGYK
Sbjct: 61  DQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSLDHPLVNPSPFLINSLVLTIQGYK 120

Query: 121 CNATNCHIQVSFSLHYIRKIPIDGLTMASPEYSVAVL 157
           CNATNCHIQVSFSLHYIRKIPIDGLTMASPEYSVAVL
Sbjct: 121 CNATNCHIQVSFSLHYIRKIPIDGLTMASPEYSVAVL 157

[Close]
BLAST of CSPI01G24410 vs. ExPASy TrEMBL
Match: A0A1S3C5W1 (putative tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497029 PE=3 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 1.7e-34
Identity = 80/102 (78.43%), Postives = 85/102 (83.33%), Query Frame = 0 

Query: 1   MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSS-TASTDAFSTRCAP 60
           MSVSSLRLPLSPWITK+SSSCFRFP PVFV RTP LRP LL+ FSS  ASTDAFST+CAP
Sbjct: 1   MSVSSLRLPLSPWITKSSSSCFRFPYPVFVPRTPRLRPTLLYCFSSAAASTDAFSTQCAP 60

Query: 61  DQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSLDHPLV 102
           DQLRKWQAFRKKKVVLRIGYVGTDYRG    +    L HP +
Sbjct: 61  DQLRKWQAFRKKKVVLRIGYVGTDYRG--LQMQRNQLSHPTI 100

[Close]
BLAST of CSPI01G24410 vs. ExPASy TrEMBL
Match: A0A6J1I156 (putative tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111469569 PE=3 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 5.9e-27
Identity = 67/87 (77.01%), Postives = 71/87 (81.61%), Query Frame = 0 

Query: 1  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALL-HFFSSTASTDAFSTRCAP 60
          M+VSSLR PLSPW TK S  CFRFP P FVS T  LRP LL    SSTASTDA ST+CAP
Sbjct: 1  MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLRPRLLCCSSSSTASTDALSTQCAP 60

Query: 61 DQLRKWQAFRKKKVVLRIGYVGTDYRG 87
          DQLRKW+AFRKKKVV+RIGYVGTDYRG
Sbjct: 61 DQLRKWEAFRKKKVVMRIGYVGTDYRG 87

[Close]
BLAST of CSPI01G24410 vs. ExPASy TrEMBL
Match: A0A6J1HM83 (putative tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111464888 PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 1.1e-25
Identity = 65/87 (74.71%), Postives = 69/87 (79.31%), Query Frame = 0 

Query: 1  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALL-HFFSSTASTDAFSTRCAP 60
          M+VSSLR PLSPW  K S  CFRFP P FVS T  L P LL    SSTASTDA ST+CAP
Sbjct: 1  MAVSSLRFPLSPWANKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60

Query: 61 DQLRKWQAFRKKKVVLRIGYVGTDYRG 87
          DQLRKW+AFRKKKVV+RIGYVGTDYRG
Sbjct: 61 DQLRKWEAFRKKKVVMRIGYVGTDYRG 87

[Close]
BLAST of CSPI01G24410 vs. ExPASy TrEMBL
Match: A0A6J1DMV6 (putative tRNA pseudouridine synthase isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022025 PE=3 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 4.2e-25
Identity = 65/87 (74.71%), Postives = 71/87 (81.61%), Query Frame = 0 

Query: 1  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSS-TASTDAFSTRCAP 60
          M+VSSLR PLSPW TK SS+CFR P   FVSR+P LRP LL   SS +ASTDA STRCAP
Sbjct: 1  MAVSSLRFPLSPW-TKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAP 60

Query: 61 DQLRKWQAFRKKKVVLRIGYVGTDYRG 87
          DQLR W+AFRKKKVV+RIGY GTDYRG
Sbjct: 61 DQLRTWEAFRKKKVVMRIGYAGTDYRG 86

[Close]
BLAST of CSPI01G24410 vs. NCBI nr
Match: XP_031742713.1 (putative tRNA pseudouridine synthase isoform X1 [Cucumis sativus])

HSP 1 Score: 177.2 bits (448), Expect = 1.1e-40
Identity = 91/111 (81.98%), Postives = 95/111 (85.59%), Query Frame = 0 

Query: 1   MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFF-SSTASTDAFSTRCAP 60
           MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFF SSTASTDAFSTRCAP
Sbjct: 1   MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSSTASTDAFSTRCAP 60

Query: 61  DQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSLDHPLVNPSPFLINS 111
           DQLRKWQAFRKKKVVLRIGYVGTDYRG    +    L HP  N +  L ++
Sbjct: 61  DQLRKWQAFRKKKVVLRIGYVGTDYRG--LQMQRNQLSHPRFNSTTNLFHN 109

[Close]
BLAST of CSPI01G24410 vs. NCBI nr
Match: XP_004145230.1 (putative tRNA pseudouridine synthase isoform X2 [Cucumis sativus] >KGN66289.2 hypothetical protein Csa_023335 [Cucumis sativus])

HSP 1 Score: 176.0 bits (445), Expect = 2.5e-40
Identity = 89/102 (87.25%), Postives = 91/102 (89.22%), Query Frame = 0 

Query: 1   MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFF-SSTASTDAFSTRCAP 60
           MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFF SSTASTDAFSTRCAP
Sbjct: 1   MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSSTASTDAFSTRCAP 60

Query: 61  DQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSLDHPLV 102
           DQLRKWQAFRKKKVVLRIGYVGTDYRG    +    L HP +
Sbjct: 61  DQLRKWQAFRKKKVVLRIGYVGTDYRG--LQMQRNQLSHPTI 100

[Close]
BLAST of CSPI01G24410 vs. NCBI nr
Match: XP_008457308.1 (PREDICTED: putative tRNA pseudouridine synthase [Cucumis melo])

HSP 1 Score: 155.6 bits (392), Expect = 3.5e-34
Identity = 80/102 (78.43%), Postives = 85/102 (83.33%), Query Frame = 0 

Query: 1   MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSS-TASTDAFSTRCAP 60
           MSVSSLRLPLSPWITK+SSSCFRFP PVFV RTP LRP LL+ FSS  ASTDAFST+CAP
Sbjct: 1   MSVSSLRLPLSPWITKSSSSCFRFPYPVFVPRTPRLRPTLLYCFSSAAASTDAFSTQCAP 60

Query: 61  DQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSLDHPLV 102
           DQLRKWQAFRKKKVVLRIGYVGTDYRG    +    L HP +
Sbjct: 61  DQLRKWQAFRKKKVVLRIGYVGTDYRG--LQMQRNQLSHPTI 100

[Close]
BLAST of CSPI01G24410 vs. NCBI nr
Match: XP_038894776.1 (putative tRNA pseudouridine synthase isoform X3 [Benincasa hispida])

HSP 1 Score: 136.3 bits (342), Expect = 2.2e-28
Identity = 70/87 (80.46%), Postives = 74/87 (85.06%), Query Frame = 0 

Query: 1  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLH-FFSSTASTDAFSTRCAP 60
          M+VSSLRLPLSPWITK SSSCF FP P FVSRT  L P L     SSTASTDAFST+CAP
Sbjct: 1  MAVSSLRLPLSPWITKTSSSCFLFPYPTFVSRTHRLGPTLSGCSASSTASTDAFSTQCAP 60

Query: 61 DQLRKWQAFRKKKVVLRIGYVGTDYRG 87
          +QLRKW+AFRKKKVVLRIGYVGTDYRG
Sbjct: 61 NQLRKWEAFRKKKVVLRIGYVGTDYRG 87

[Close]
BLAST of CSPI01G24410 vs. NCBI nr
Match: XP_038894779.1 (putative tRNA pseudouridine synthase isoform X5 [Benincasa hispida])

HSP 1 Score: 136.3 bits (342), Expect = 2.2e-28
Identity = 70/87 (80.46%), Postives = 74/87 (85.06%), Query Frame = 0 

Query: 1  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLH-FFSSTASTDAFSTRCAP 60
          M+VSSLRLPLSPWITK SSSCF FP P FVSRT  L P L     SSTASTDAFST+CAP
Sbjct: 1  MAVSSLRLPLSPWITKTSSSCFLFPYPTFVSRTHRLGPTLSGCSASSTASTDAFSTQCAP 60

Query: 61 DQLRKWQAFRKKKVVLRIGYVGTDYRG 87
          +QLRKW+AFRKKKVVLRIGYVGTDYRG
Sbjct: 61 NQLRKWEAFRKKKVVLRIGYVGTDYRG 87

[Close]
BLAST of CSPI01G24410 vs. TAIR 10
Match: AT2G30320.1 (Pseudouridine synthase family protein )

HSP 1 Score: 60.5 bits (145), Expect = 1.4e-09
Identity = 41/105 (39.05%), Postives = 56/105 (53.33%), Query Frame = 0 

Query: 1   MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSTASTDAFSTRCAPD 60
           M+VS LR+P+        S  F +P     +R   + PALL F SS A   + ++   P 
Sbjct: 1   MAVSFLRIPI-------PSRVFWYP-----TRLIRVTPALLRFSSSAAFQPSSTSLSPPS 60

Query: 61  Q----LRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSLDHPLV 102
                  KW+++RKKKVV+RIGYVGTDYRG     L +  D P +
Sbjct: 61  SDNFLADKWESYRKKKVVIRIGYVGTDYRG-----LQIQRDDPSI 88

The following BLAST results are available for this feature:
BLAST of CSPI01G24410 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O229282.0e-0839.05Putative tRNA pseudouridine synthase OS=Arabidopsis thaliana OX=3702 GN=At2g3032... [more]
BLAST of CSPI01G24410 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LVB63.4e-8399.36Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G533670 PE=4 SV=1[more]
A0A1S3C5W11.7e-3478.43putative tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497029 PE=... [more]
A0A6J1I1565.9e-2777.01putative tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111469569... [more]
A0A6J1HM831.1e-2574.71putative tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC1114648... [more]
A0A6J1DMV64.2e-2574.71putative tRNA pseudouridine synthase isoform X2 OS=Momordica charantia OX=3673 G... [more]
BLAST of CSPI01G24410 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_031742713.11.1e-4081.98putative tRNA pseudouridine synthase isoform X1 [Cucumis sativus][more]
XP_004145230.12.5e-4087.25putative tRNA pseudouridine synthase isoform X2 [Cucumis sativus] >KGN66289.2 hy... [more]
XP_008457308.13.5e-3478.43PREDICTED: putative tRNA pseudouridine synthase [Cucumis melo][more]
XP_038894776.12.2e-2880.46putative tRNA pseudouridine synthase isoform X3 [Benincasa hispida][more]
XP_038894779.12.2e-2880.46putative tRNA pseudouridine synthase isoform X5 [Benincasa hispida][more]
BLAST of CSPI01G24410 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G30320.11.4e-0939.05Pseudouridine synthase family protein [more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G24410.1CSPI01G24410.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031119 tRNA pseudouridine synthesis
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding