CSPI01G24370 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G24370
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionStructure-specific endonuclease subunit SLX1 homolog
LocationChr1: 19679405 .. 19682465 (+)
RNA-Seq ExpressionCSPI01G24370
SyntenyCSPI01G24370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCATTCTGACGTGTCCATATGCCCTAAGTCCAATTTTTATAATTTAAATTATTATTTCATTGGGAATACAAAAAAAAATAAAAAAATCTCTTGTGTGAATTAGGGCAAAACGGTAAATTCACGAACCCAACTCAATTTTGCTGTAATGAAGTGATTTCAAAATCCAAAAATACCATATTTTTTACCAACGGCCGAATTGTATTTTTGAGTAAAATTTTCATCTTCTACGGAGGCAGAGAGAGAGAGAGAGAGATGCGGAAGAGGAAAGAGAAACCGGAAATCTGTAAAACCATGGATGAGGAGAAGGAAGACGATGAAGAAGAAGAGAGAGGAAACGAAGTGAATGGCTTCTTTTCATGCTATCTCTTGGCCTCTGCTTGCCCTCGTTTCAAAGGCCATACCTATATCGGGTTTGAAACTTTCCCACTCCATTTTTAGCTCCTTGCTTCCCTACATCGGACCTTCTATTTGCTTTCTAAATTCCCAATTTTCGAATTTCTTTACCATCTTTGATTCGGTTCTTTTGTAAAGATTGAGTGAGGGAAAACATAAAGAAATGTTGGTGAGTCGATCTGATCTGCTGGGTATTGATTTGTTGACTGTTTATATGAAATTTTAGATTCACAGTGAACCCGAAGCGCAGAATCAGACAACACAATGGTGAGATTAGATGCGGTGCTTGGCGTACTAAGAGGAAGAGACCTTGGGAGATGGTCTTGTGCATTTATGGCTTCCCTACTAATGTTTCTGCTCTTCAGGTTACATTTCCTGACTTTCAGCTCTAAACTAGTTTGATTCTGCGTTTTTTCTACTTGATTCTCTTTTACTAGCTGAATGAATGACACTTCGATGATTATGCAAATGTTGAGATGTTAGATTAAAAGAAGAAAAAAAGCAGATTCTCTTTCAGAACTCATTTTTTTTATCATAAGAAATAGTGGCTTGCATATTAGGGATTTTATGGCTTAAAGAATATACCAAAATTTGTTGCTGCTTTAATTTTCTTCTTTAGCCGTTTACTTTCTTGTAAGTTTCCATCAGTTCTAGAATTTGATTTTCTAAACTGTGGGTACAGTTTGAATGGGCGTGGCAGCATCCTAATGAGTCACTGGCTGTAAGAAGTGCTGCTGCAACTTTCAAGTCTCTCTCTGGAGTTGCTAACAAAGTTAAACTTGCATACACAATGCTCACACTTCCTGCTTGGCGTGGGTATGACATTTGAAAAAATTTATACAATCTTGTCGAATTTATCTGTTAAATATGCGAGAAGCGTGAAGAGAAACCTAAAAATAATGAAAATATCCATCAACATGTATTGAAAGGTTTGACATTGATATTAAATATTATTGAACATCTCCAATATCTTCTATAAATGCTTGTAAACATCAAATCTTCAAATCGATGTGGACATGAGTATTTTAATCCTTGGATAATCCTCCATATCAGCGAGAAAAGGGAAAGATTTATAGTATCTCAATAAAGACAACAATCTTATAACTGTCTTGTTTTTGGGGCAAAACCAAAGTTTGAGAGTGAAAAGGGCGATATCTTATTACTGTGATTTATGAAGGAGCAACAAACTGATGTAATTTCAATCAATTTTGTCTGGCAATCTCTTTATTCATTTATTTCTCCTTATCCTTGCAGTTTAAATATAACTGTAAACTACTTCTCTACAAAGTTCATAAAGAATGCGGCTGGTTGCCCAAGTTTGCCAGAGCATATGAAGGTCCAAGTTTCCCCCATCAATGAGCTTCCATGCTATTCCGAAGGAGATCAAGATATGCTTGAAAATGAAGGTGACTGGGAGTATAATCGAGAACGTGAAGAAATTTGTGGTTTTCGAGTGTATGGTTCAATGAAAGAAGTTTCAAATGAAGTTCCTCAAAAGTTAATGGATTATCAGACAGGTACAGATGGAAGACCTCCTCACGTGTTGCGTGGATGTGATAAGGAGCTTGAGACCAATGAACAAGTGCCACCTTCTTCATGTACTCCGTCTTATATTGATGTGGGTATGTCTTATGACTTGTGTGCATGTGATGAAGGACTCGAGAATGATGAAAGAGAGGCAGCTTCGTGTGGTCAGTCTTGCATTGTAGCAGGTACATCTAGAACTGAAATTGTCATTGATGACGAAGAGGAAAACCAATTAGAAGGGAGTAGTATGAACTTGCAGAAGCAACCAGGTAGGGAGAATTTAACATCAGGAATAGCTTCTGAAATCAGTAAAGTTTCAAGGTGGAACAATGGCTGGGTTCCAACTGTTGAGTATGAGGTTATCGATGTGTCTACCCCGTCTCCAGACTGCCGAACAAGTTCGCATAGATTCAAGAGAAGAGTTACTTCCGGTAAGTCTGAGATGATTGACTTGACTAAATCTCCTACATTTATTCAATTGTAGAAGGATGGATGGATTTGGATTATTGTGCATATAGGAGATATGGAATGAGTTTTCATGAGAAGTTTCATTTTTGCAATGAAGAGGTAGTATTACTATAACAGATAGCATCTTCTCAACCCTTTTCAGAGATAACGTGGGCTCAACTATGGTTGACTAGACCAAATAAGAGGGGAACATGTTGTCAAGTTATATATAATAATTATAATAACAGCCTCTCTTGCAAAGGCATCATTTTAAAGGGGAATCTAAGTCTGAAAAATAACATTGAATCGGTTGTGGTATCAATTAATTTGTTTCAAACGACCGGGATCAATGTTCAACTATGCAACTACACCTTGATTACATATCCATCTATACTACTATATATGGATGGAGTGTAGATGAGAGAATGTTTTTTACTTTTTTCTCTTTGCTCGATTCACATTCCTTCCTTGTATGTACCTTTCCTGTTTTGACAAGGAATGACAATGGAAGAGAGATTATGAGTTTATAGAGGCATATGAACAGAAATTCAAGCAAGGAGAAAATGAAGGTGAATCATCTAAGTGATAGTTGTATCGTTCTTAAAATTTAGAAAGAAAAAAAAAATCTATCACCACCGCTCATATTGATAAACTTAAAAAATTGCGATTCATAATTTATAGTTTTTCTTTT

mRNA sequence

ATGCCATTCTGACGTGTCCATATGCCCTAAGTCCAATTTTTATAATTTAAATTATTATTTCATTGGGAATACAAAAAAAAATAAAAAAATCTCTTGTGTGAATTAGGGCAAAACGGTAAATTCACGAACCCAACTCAATTTTGCTGTAATGAAGTGATTTCAAAATCCAAAAATACCATATTTTTTACCAACGGCCGAATTGTATTTTTGAGTAAAATTTTCATCTTCTACGGAGGCAGAGAGAGAGAGAGAGAGATGCGGAAGAGGAAAGAGAAACCGGAAATCTGTAAAACCATGGATGAGGAGAAGGAAGACGATGAAGAAGAAGAGAGAGGAAACGAAGTGAATGGCTTCTTTTCATGCTATCTCTTGGCCTCTGCTTGCCCTCGTTTCAAAGGCCATACCTATATCGGATTCACAGTGAACCCGAAGCGCAGAATCAGACAACACAATGGTGAGATTAGATGCGGTGCTTGGCGTACTAAGAGGAAGAGACCTTGGGAGATGGTCTTGTGCATTTATGGCTTCCCTACTAATGTTTCTGCTCTTCAGTTTGAATGGGCGTGGCAGCATCCTAATGAGTCACTGGCTGTAAGAAGTGCTGCTGCAACTTTCAAGTCTCTCTCTGGAGTTGCTAACAAAGTTAAACTTGCATACACAATGCTCACACTTCCTGCTTGGCGTGGTTTAAATATAACTGTAAACTACTTCTCTACAAAGTTCATAAAGAATGCGGCTGGTTGCCCAAGTTTGCCAGAGCATATGAAGGTCCAAGTTTCCCCCATCAATGAGCTTCCATGCTATTCCGAAGGAGATCAAGATATGCTTGAAAATGAAGGTGACTGGGAGTATAATCGAGAACGTGAAGAAATTTGTGGTTTTCGAGTGTATGGTTCAATGAAAGAAGTTTCAAATGAAGTTCCTCAAAAGTTAATGGATTATCAGACAGGTACAGATGGAAGACCTCCTCACGTGTTGCGTGGATGTGATAAGGAGCTTGAGACCAATGAACAAGTGCCACCTTCTTCATGTACTCCGTCTTATATTGATGTGGGTATGTCTTATGACTTGTGTGCATGTGATGAAGGACTCGAGAATGATGAAAGAGAGGCAGCTTCGTGTGGTCAGTCTTGCATTGTAGCAGGTACATCTAGAACTGAAATTGTCATTGATGACGAAGAGGAAAACCAATTAGAAGGGAGTAGTATGAACTTGCAGAAGCAACCAGGTAGGGAGAATTTAACATCAGGAATAGCTTCTGAAATCAGTAAAGTTTCAAGGTGGAACAATGGCTGGGTTCCAACTGTTGAGTATGAGGTTATCGATGTGTCTACCCCGTCTCCAGACTGCCGAACAAGTTCGCATAGATTCAAGAGAAGAGTTACTTCCGGTAAGTCTGAGATGATTGACTTGACTAAATCTCCTACATTTATTCAATTGTAGAAGGATGGATGGATTTGGATTATTGTGCATATAGGAGATATGGAATGAGTTTTCATGAGAAGTTTCATTTTTGCAATGAAGAGGTAGTATTACTATAACAGATAGCATCTTCTCAACCCTTTTCAGAGATAACGTGGGCTCAACTATGGTTGACTAGACCAAATAAGAGGGGAACATGTTGTCAAGTTATATATAATAATTATAATAACAGCCTCTCTTGCAAAGGCATCATTTTAAAGGGGAATCTAAGTCTGAAAAATAACATTGAATCGGTTGTGGTATCAATTAATTTGTTTCAAACGACCGGGATCAATGTTCAACTATGCAACTACACCTTGATTACATATCCATCTATACTACTATATATGGATGGAGTGTAGATGAGAGAATGTTTTTTACTTTTTTCTCTTTGCTCGATTCACATTCCTTCCTTGTATGTACCTTTCCTGTTTTGACAAGGAATGACAATGGAAGAGAGATTATGAGTTTATAGAGGCATATGAACAGAAATTCAAGCAAGGAGAAAATGAAGGTGAATCATCTAAGTGATAGTTGTATCGTTCTTAAAATTTAGAAAGAAAAAAAAAATCTATCACCACCGCTCATATTGATAAACTTAAAAAATTGCGATTCATAATTTATAGTTTTTCTTTT

Coding sequence (CDS)

ATGCGGAAGAGGAAAGAGAAACCGGAAATCTGTAAAACCATGGATGAGGAGAAGGAAGACGATGAAGAAGAAGAGAGAGGAAACGAAGTGAATGGCTTCTTTTCATGCTATCTCTTGGCCTCTGCTTGCCCTCGTTTCAAAGGCCATACCTATATCGGATTCACAGTGAACCCGAAGCGCAGAATCAGACAACACAATGGTGAGATTAGATGCGGTGCTTGGCGTACTAAGAGGAAGAGACCTTGGGAGATGGTCTTGTGCATTTATGGCTTCCCTACTAATGTTTCTGCTCTTCAGTTTGAATGGGCGTGGCAGCATCCTAATGAGTCACTGGCTGTAAGAAGTGCTGCTGCAACTTTCAAGTCTCTCTCTGGAGTTGCTAACAAAGTTAAACTTGCATACACAATGCTCACACTTCCTGCTTGGCGTGGTTTAAATATAACTGTAAACTACTTCTCTACAAAGTTCATAAAGAATGCGGCTGGTTGCCCAAGTTTGCCAGAGCATATGAAGGTCCAAGTTTCCCCCATCAATGAGCTTCCATGCTATTCCGAAGGAGATCAAGATATGCTTGAAAATGAAGGTGACTGGGAGTATAATCGAGAACGTGAAGAAATTTGTGGTTTTCGAGTGTATGGTTCAATGAAAGAAGTTTCAAATGAAGTTCCTCAAAAGTTAATGGATTATCAGACAGGTACAGATGGAAGACCTCCTCACGTGTTGCGTGGATGTGATAAGGAGCTTGAGACCAATGAACAAGTGCCACCTTCTTCATGTACTCCGTCTTATATTGATGTGGGTATGTCTTATGACTTGTGTGCATGTGATGAAGGACTCGAGAATGATGAAAGAGAGGCAGCTTCGTGTGGTCAGTCTTGCATTGTAGCAGGTACATCTAGAACTGAAATTGTCATTGATGACGAAGAGGAAAACCAATTAGAAGGGAGTAGTATGAACTTGCAGAAGCAACCAGGTAGGGAGAATTTAACATCAGGAATAGCTTCTGAAATCAGTAAAGTTTCAAGGTGGAACAATGGCTGGGTTCCAACTGTTGAGTATGAGGTTATCGATGTGTCTACCCCGTCTCCAGACTGCCGAACAAGTTCGCATAGATTCAAGAGAAGAGTTACTTCCGGTAAGTCTGAGATGATTGACTTGACTAAATCTCCTACATTTATTCAATTGTAG

Protein sequence

MRKRKEKPEICKTMDEEKEDDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFIKNAAGCPSLPEHMKVQVSPINELPCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGRPPHVLRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAGTSRTEIVIDDEEENQLEGSSMNLQKQPGRENLTSGIASEISKVSRWNNGWVPTVEYEVIDVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL*
Homology
BLAST of CSPI01G24370 vs. ExPASy Swiss-Prot
Match: Q8BX32 (Structure-specific endonuclease subunit SLX1 OS=Mus musculus OX=10090 GN=Slx1b PE=2 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.8e-29
Identity = 67/164 (40.85%), Postives = 92/164 (56.10%), Query Frame = 0

Query: 33  FFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHN-GEIRCGAWRTKRKRPWEMVLCIYGF 92
           FF  YLL    PR +G  Y+GFTVNP RR+RQHN G  + GAWRT  + PW+MVL I+GF
Sbjct: 10  FFGVYLLYCQNPRHRGRVYVGFTVNPARRVRQHNAGRKKGGAWRTSGRGPWDMVLIIHGF 69

Query: 93  PTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNY 152
           P+ V+AL+FEWAWQHP  S  +       +S +  A  +++   ML +P W  L +T+ +
Sbjct: 70  PSAVAALRFEWAWQHPQASRRLTHVGPRLRSEAAFAFHLRVLAHMLRVPPWVRLPLTLRW 129

Query: 153 FSTKFIKNAAGCPSLPEHMKVQVSP------INELPCYSEGDQD 190
               F      CP+ P HM +   P      + + P  SE D +
Sbjct: 130 LRPDFRHEL--CPAPPAHMPIAFGPPPPQPLVPKRPAVSEADSE 171

BLAST of CSPI01G24370 vs. ExPASy Swiss-Prot
Match: Q5PQP5 (Structure-specific endonuclease subunit SLX1 OS=Rattus norvegicus OX=10116 GN=Slx1b PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 5.2e-29
Identity = 66/164 (40.24%), Postives = 91/164 (55.49%), Query Frame = 0

Query: 33  FFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHN-GEIRCGAWRTKRKRPWEMVLCIYGF 92
           FF  YLL    PR +G  Y+GFTVNP RR+RQHN G  + GAWRT  + PW+MVL ++GF
Sbjct: 10  FFGVYLLYCQNPRHRGRVYVGFTVNPARRVRQHNAGRKKGGAWRTSGRGPWDMVLILHGF 69

Query: 93  PTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNY 152
           P+ V+AL+FEWAWQHP  S  +       +S +     +++   ML +P W  L +TV +
Sbjct: 70  PSAVAALRFEWAWQHPQASRRLTHVGPRLRSEASFTFHLRVLAHMLRVPPWVRLPLTVRW 129

Query: 153 FSTKFIKNAAGCPSLPEHMKVQVSP------INELPCYSEGDQD 190
               F      CP+ P HM +   P      + + P  SE D +
Sbjct: 130 LRPDFRHEL--CPAPPPHMPIAFGPPPPQPLVPKRPAASEADSE 171

BLAST of CSPI01G24370 vs. ExPASy Swiss-Prot
Match: Q32PI0 (Structure-specific endonuclease subunit SLX1 OS=Bos taurus OX=9913 GN=SLX1A PE=2 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 9.9e-28
Identity = 61/145 (42.07%), Postives = 84/145 (57.93%), Query Frame = 0

Query: 33  FFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHN-GEIRCGAWRTKRKRPWEMVLCIYGF 92
           FF  YLL    PR +G  Y+GFTVNP RR++QHN G  + GAWRT  + PWEMVL ++GF
Sbjct: 6   FFGVYLLYCLNPRHRGRVYVGFTVNPARRVQQHNGGRKKGGAWRTSGRGPWEMVLIVHGF 65

Query: 93  PTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNY 152
           P+ V+AL+FEWAWQHP  S  +       +  +  A  +++   ML  P W  L +T+ +
Sbjct: 66  PSAVAALRFEWAWQHPQASRRLTHVGRRLRGEATFAFHLRVLAHMLRAPPWVRLPLTLRW 125

Query: 153 FSTKFIKNAAGCPSLPEHMKVQVSP 177
               F ++   CP  P HM +   P
Sbjct: 126 LRADFRQDL--CPPPPPHMPLAFGP 148

BLAST of CSPI01G24370 vs. ExPASy Swiss-Prot
Match: Q0IH86 (Structure-specific endonuclease subunit slx1 OS=Xenopus laevis OX=8355 GN=slx1a PE=2 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 4.9e-27
Identity = 59/154 (38.31%), Postives = 88/154 (57.14%), Query Frame = 0

Query: 29  EVNGFFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHN-GEIRCGAWRTKRKRPWEMVLC 88
           EV GF+  YLL    P++KG  YIGFTVNP+RRI+QHN G+ + GAW+T  + PW+MVL 
Sbjct: 4   EVEGFYGVYLLFCTNPKYKGRIYIGFTVNPERRIQQHNGGKHKGGAWKTSGRGPWDMVLI 63

Query: 89  IYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWRGLNI 148
           ++GFP +++AL+FEWAWQHP+ S  +       K  S     + +   ML +  W  L +
Sbjct: 64  VHGFPNDIAALRFEWAWQHPHVSRRLTHVPRKTKKQSSFDFHLLVLCHMLRVAPWNRLPL 123

Query: 149 TVNYFSTKFIKNAAGCPSLPEHMKVQVSPINELP 182
           T+ +   ++ +        P HM +    +   P
Sbjct: 124 TLRWLRQEYRRELPLLLQPPLHMPLAFGQVRARP 157

BLAST of CSPI01G24370 vs. ExPASy Swiss-Prot
Match: A7STV9 (Structure-specific endonuclease subunit SLX1 homolog OS=Nematostella vectensis OX=45351 GN=v1g174298 PE=3 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 2.4e-26
Identity = 61/149 (40.94%), Postives = 87/149 (58.39%), Query Frame = 0

Query: 31  NGFFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHNGEI-RCGAWRTKRKRPWEMVLCIY 90
           +GF   YLL    P+FKGHTYIG+TVNP RRI+QHNG + + GA++T RK+PW M+L ++
Sbjct: 8   SGFHGVYLLYCVNPKFKGHTYIGYTVNPNRRIKQHNGGVDKGGAYKTSRKKPWNMILIVH 67

Query: 91  GFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLS-GVANKVKLAYTMLTLPAWRGLNIT 150
           GFP ++ ALQFEWAWQ P  S  ++SAA   K     V    ++   +L +  W  L + 
Sbjct: 68  GFPNDIIALQFEWAWQKPTVSRRLKSAATKKKPRERPVQYCFRILSELLRVGPWNRLPLH 127

Query: 151 VNYFSTKFIKNAAGCPSLPEHMKVQVSPI 178
           + +   ++          P HM V   P+
Sbjct: 128 IRWLMREYEMEFDPRCLPPFHMTVVYGPL 156

BLAST of CSPI01G24370 vs. ExPASy TrEMBL
Match: A0A0A0M0Y6 (Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis sativus OX=3659 GN=Csa_1G533630 PE=3 SV=1)

HSP 1 Score: 812.8 bits (2098), Expect = 6.5e-232
Identity = 392/395 (99.24%), Postives = 394/395 (99.75%), Query Frame = 0

Query: 1   MRKRKEKPEICKTMDEEKEDDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVNPKR 60
           MRKRKEKPEICKT DEEKEDDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVNPKR
Sbjct: 1   MRKRKEKPEICKTTDEEKEDDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVNPKR 60

Query: 61  RIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATF 120
           RIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATF
Sbjct: 61  RIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATF 120

Query: 121 KSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFIKNAAGCPSLPEHMKVQVSPINEL 180
           KSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKF+KNAAGCPSLPEHMKVQVSPINEL
Sbjct: 121 KSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPINEL 180

Query: 181 PCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGRPPHV 240
           PCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGRPPHV
Sbjct: 181 PCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGRPPHV 240

Query: 241 LRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAGTSR 300
           LRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAGTSR
Sbjct: 241 LRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAGTSR 300

Query: 301 TEIVIDDEEENQLEGSSMNLQKQPGRENLTSGIASEISKVSRWNNGWVPTVEYEVIDVST 360
           TEIVIDDEEENQLEGSSMNLQ+QPGRENLTSGIASEISKVSRWNNGWVPTVEYEVIDVST
Sbjct: 301 TEIVIDDEEENQLEGSSMNLQEQPGRENLTSGIASEISKVSRWNNGWVPTVEYEVIDVST 360

Query: 361 PSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 396
           PSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL
Sbjct: 361 PSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 395

BLAST of CSPI01G24370 vs. ExPASy TrEMBL
Match: A0A1S3C574 (Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis melo OX=3656 GN=LOC103497035 PE=3 SV=1)

HSP 1 Score: 755.7 bits (1950), Expect = 9.4e-215
Identity = 372/401 (92.77%), Postives = 382/401 (95.26%), Query Frame = 0

Query: 1   MRKRKEKPEICKTM-----DEEKE-DDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGF 60
           MRKRKEKPEICKTM     DEEKE DDEEEERGN VNGFFSCYLLASACPRFKGHTYIGF
Sbjct: 1   MRKRKEKPEICKTMAAVVQDEEKEDDDEEEERGNGVNGFFSCYLLASACPRFKGHTYIGF 60

Query: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR 120
           TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR
Sbjct: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR 120

Query: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFIKNAAGCPSLPEHMKVQV 180
           SAAATFKSLSGVANKVKLAYTMLTLPAW GLNITVNYFSTKF+KNAAGCPSLPEHMKVQV
Sbjct: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWCGLNITVNYFSTKFMKNAAGCPSLPEHMKVQV 180

Query: 181 SPINELPCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTD 240
           SPI+ELPCYSEGDQ +LENE DWEY+RE EEIC F+VYGSMKEVSNEVPQKLMDYQTGTD
Sbjct: 181 SPIDELPCYSEGDQGVLENEDDWEYDRESEEICSFQVYGSMKEVSNEVPQKLMDYQTGTD 240

Query: 241 GRPPHVLRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCI 300
           GRPP VLRGCDKELETNEQVPPSSCTPSY+DVGMSYDLCACDEGLENDEREAASCGQSC 
Sbjct: 241 GRPPPVLRGCDKELETNEQVPPSSCTPSYVDVGMSYDLCACDEGLENDEREAASCGQSCT 300

Query: 301 VAGTSRTEIVIDDEEENQLEGSSMNLQKQPGRENLTSGIASEISKVSRWNNGWVPTVEYE 360
           VAGTSRTEI+IDD EENQ EGS MNLQ+QPGR+NLTSGIASEISKVSR +NG VPTVEYE
Sbjct: 301 VAGTSRTEIIIDDGEENQFEGSGMNLQEQPGRKNLTSGIASEISKVSRCSNGLVPTVEYE 360

Query: 361 VIDVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 396
           VIDVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL
Sbjct: 361 VIDVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 401

BLAST of CSPI01G24370 vs. ExPASy TrEMBL
Match: A0A5D3BBR0 (Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G00560 PE=3 SV=1)

HSP 1 Score: 736.1 bits (1899), Expect = 7.7e-209
Identity = 358/382 (93.72%), Postives = 368/382 (96.34%), Query Frame = 0

Query: 15  DEEKE-DDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHNGEIRCGA 74
           DEEKE DDEEEERGN VNGFFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHNGEIRCGA
Sbjct: 7   DEEKEDDDEEEERGNGVNGFFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHNGEIRCGA 66

Query: 75  WRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLA 134
           WRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLA
Sbjct: 67  WRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLA 126

Query: 135 YTMLTLPAWRGLNITVNYFSTKFIKNAAGCPSLPEHMKVQVSPINELPCYSEGDQDMLEN 194
           YTMLTLPAW GLNITVNYFSTKF+KNAAGCPSLPEHMKVQVSPI+ELPCYSEGDQ +LEN
Sbjct: 127 YTMLTLPAWCGLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPIDELPCYSEGDQGVLEN 186

Query: 195 EGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGRPPHVLRGCDKELETNEQ 254
           E DWEY+RE EEIC F+VYGSMKEVSNEVPQKLMDYQTGTDGRPP VLRGCDKELETNEQ
Sbjct: 187 EDDWEYDRESEEICSFQVYGSMKEVSNEVPQKLMDYQTGTDGRPPPVLRGCDKELETNEQ 246

Query: 255 VPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAGTSRTEIVIDDEEENQL 314
           VPPSSCTPSY+DVGMSYDLCACDEGLENDEREAASCGQSC VAGTSRTEI+IDD EENQ 
Sbjct: 247 VPPSSCTPSYVDVGMSYDLCACDEGLENDEREAASCGQSCTVAGTSRTEIIIDDGEENQF 306

Query: 315 EGSSMNLQKQPGRENLTSGIASEISKVSRWNNGWVPTVEYEVIDVSTPSPDCRTSSHRFK 374
           EGS MNLQ+QPGR+NLTSGIASEISKVSR +NG VPTVEYEVIDVSTPSPDCRTSSHRFK
Sbjct: 307 EGSGMNLQEQPGRKNLTSGIASEISKVSRCSNGLVPTVEYEVIDVSTPSPDCRTSSHRFK 366

Query: 375 RRVTSGKSEMIDLTKSPTFIQL 396
           RRVTSGKSEMIDLTKSPTFIQL
Sbjct: 367 RRVTSGKSEMIDLTKSPTFIQL 388

BLAST of CSPI01G24370 vs. ExPASy TrEMBL
Match: A0A6J1I681 (Structure-specific endonuclease subunit SLX1 homolog OS=Cucurbita maxima OX=3661 GN=LOC111469571 PE=3 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 2.2e-163
Identity = 293/379 (77.31%), Postives = 323/379 (85.22%), Query Frame = 0

Query: 1   MRKRKEKPEICKTM---DEEKEDDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVN 60
           MR+RKEKPEI KTM   +E+KEDDE+EERGNE NGFFSCYLLASACPR+KGHTYIGFTVN
Sbjct: 1   MRRRKEKPEIPKTMAVAEEKKEDDEDEERGNEGNGFFSCYLLASACPRYKGHTYIGFTVN 60

Query: 61  PKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAA 120
           PKRRIRQHNGEIRCGAWRTKRKRPWEMV CIYGFPTNVSALQFEWAWQHPNESLAVRSAA
Sbjct: 61  PKRRIRQHNGEIRCGAWRTKRKRPWEMVFCIYGFPTNVSALQFEWAWQHPNESLAVRSAA 120

Query: 121 ATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFIKNAAGCPSLPEHMKVQVSPI 180
           ATFKSLSG+ANK+KLAYTMLTLPAW  LNITVNYFSTK++ NAAGCPSLP HM+VQ+SPI
Sbjct: 121 ATFKSLSGIANKIKLAYTMLTLPAWCSLNITVNYFSTKYMNNAAGCPSLPGHMRVQISPI 180

Query: 181 NELPCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGRP 240
           +ELPCYSEGD+ +LENE DWEYN+E EE+C  +V+G M EV NE+PQ LMDY TGTDGR 
Sbjct: 181 DELPCYSEGDRCVLENENDWEYNKEYEEVCSSQVHGLMTEVPNEIPQTLMDYSTGTDGR- 240

Query: 241 PHVLRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAG 300
           PH LRG D+EL+ NEQVP SSCTPSYIDVG+SYDL  C      DE+E A C Q C V G
Sbjct: 241 PHELRGSDEELDDNEQVPHSSCTPSYIDVGVSYDLRGC------DEQEPALCAQPCTVEG 300

Query: 301 TSRTEIVIDDEEENQLEGSSMNLQKQPGRENLTSGIASEISKVSRWNNGWVPTVEYEVID 360
              TEI+IDD EENQLEGS M LQ+QPGR+   SGIA+EISKVS      +P VEYEVID
Sbjct: 301 W--TEIIIDDGEENQLEGSGMKLQQQPGRKKNLSGIATEISKVSY----GLPPVEYEVID 360

Query: 361 VSTPSPDCRTSSHRFKRRV 377
           VSTPSP CRTSSH+FKRR+
Sbjct: 361 VSTPSPVCRTSSHKFKRRL 366

BLAST of CSPI01G24370 vs. ExPASy TrEMBL
Match: A0A6J1HZQ2 (Structure-specific endonuclease subunit SLX1 homolog OS=Cucurbita maxima OX=3661 GN=LOC111469571 PE=3 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 2.2e-163
Identity = 293/379 (77.31%), Postives = 323/379 (85.22%), Query Frame = 0

Query: 1   MRKRKEKPEICKTM---DEEKEDDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVN 60
           MR+RKEKPEI KTM   +E+KEDDE+EERGNE NGFFSCYLLASACPR+KGHTYIGFTVN
Sbjct: 1   MRRRKEKPEIPKTMAVAEEKKEDDEDEERGNEGNGFFSCYLLASACPRYKGHTYIGFTVN 60

Query: 61  PKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAA 120
           PKRRIRQHNGEIRCGAWRTKRKRPWEMV CIYGFPTNVSALQFEWAWQHPNESLAVRSAA
Sbjct: 61  PKRRIRQHNGEIRCGAWRTKRKRPWEMVFCIYGFPTNVSALQFEWAWQHPNESLAVRSAA 120

Query: 121 ATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFIKNAAGCPSLPEHMKVQVSPI 180
           ATFKSLSG+ANK+KLAYTMLTLPAW  LNITVNYFSTK++ NAAGCPSLP HM+VQ+SPI
Sbjct: 121 ATFKSLSGIANKIKLAYTMLTLPAWCSLNITVNYFSTKYMNNAAGCPSLPGHMRVQISPI 180

Query: 181 NELPCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGRP 240
           +ELPCYSEGD+ +LENE DWEYN+E EE+C  +V+G M EV NE+PQ LMDY TGTDGR 
Sbjct: 181 DELPCYSEGDRCVLENENDWEYNKEYEEVCSSQVHGLMTEVPNEIPQTLMDYSTGTDGR- 240

Query: 241 PHVLRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAG 300
           PH LRG D+EL+ NEQVP SSCTPSYIDVG+SYDL  C      DE+E A C Q C V G
Sbjct: 241 PHELRGSDEELDDNEQVPHSSCTPSYIDVGVSYDLRGC------DEQEPALCAQPCTVEG 300

Query: 301 TSRTEIVIDDEEENQLEGSSMNLQKQPGRENLTSGIASEISKVSRWNNGWVPTVEYEVID 360
              TEI+IDD EENQLEGS M LQ+QPGR+   SGIA+EISKVS      +P VEYEVID
Sbjct: 301 W--TEIIIDDGEENQLEGSGMKLQQQPGRKKNLSGIATEISKVSY----GLPPVEYEVID 360

Query: 361 VSTPSPDCRTSSHRFKRRV 377
           VSTPSP CRTSSH+FKRR+
Sbjct: 361 VSTPSPVCRTSSHKFKRRL 366

BLAST of CSPI01G24370 vs. NCBI nr
Match: XP_004153269.1 (structure-specific endonuclease subunit SLX1 homolog [Cucumis sativus])

HSP 1 Score: 812.8 bits (2098), Expect = 1.3e-231
Identity = 392/395 (99.24%), Postives = 394/395 (99.75%), Query Frame = 0

Query: 1   MRKRKEKPEICKTMDEEKEDDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVNPKR 60
           MRKRKEKPEICKT DEEKEDDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVNPKR
Sbjct: 1   MRKRKEKPEICKTTDEEKEDDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVNPKR 60

Query: 61  RIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATF 120
           RIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATF
Sbjct: 61  RIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATF 120

Query: 121 KSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFIKNAAGCPSLPEHMKVQVSPINEL 180
           KSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKF+KNAAGCPSLPEHMKVQVSPINEL
Sbjct: 121 KSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPINEL 180

Query: 181 PCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGRPPHV 240
           PCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGRPPHV
Sbjct: 181 PCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGRPPHV 240

Query: 241 LRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAGTSR 300
           LRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAGTSR
Sbjct: 241 LRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAGTSR 300

Query: 301 TEIVIDDEEENQLEGSSMNLQKQPGRENLTSGIASEISKVSRWNNGWVPTVEYEVIDVST 360
           TEIVIDDEEENQLEGSSMNLQ+QPGRENLTSGIASEISKVSRWNNGWVPTVEYEVIDVST
Sbjct: 301 TEIVIDDEEENQLEGSSMNLQEQPGRENLTSGIASEISKVSRWNNGWVPTVEYEVIDVST 360

Query: 361 PSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 396
           PSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL
Sbjct: 361 PSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 395

BLAST of CSPI01G24370 vs. NCBI nr
Match: XP_008457313.1 (PREDICTED: uncharacterized protein LOC103497035 [Cucumis melo])

HSP 1 Score: 755.7 bits (1950), Expect = 1.9e-214
Identity = 372/401 (92.77%), Postives = 382/401 (95.26%), Query Frame = 0

Query: 1   MRKRKEKPEICKTM-----DEEKE-DDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGF 60
           MRKRKEKPEICKTM     DEEKE DDEEEERGN VNGFFSCYLLASACPRFKGHTYIGF
Sbjct: 1   MRKRKEKPEICKTMAAVVQDEEKEDDDEEEERGNGVNGFFSCYLLASACPRFKGHTYIGF 60

Query: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR 120
           TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR
Sbjct: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR 120

Query: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFIKNAAGCPSLPEHMKVQV 180
           SAAATFKSLSGVANKVKLAYTMLTLPAW GLNITVNYFSTKF+KNAAGCPSLPEHMKVQV
Sbjct: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWCGLNITVNYFSTKFMKNAAGCPSLPEHMKVQV 180

Query: 181 SPINELPCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTD 240
           SPI+ELPCYSEGDQ +LENE DWEY+RE EEIC F+VYGSMKEVSNEVPQKLMDYQTGTD
Sbjct: 181 SPIDELPCYSEGDQGVLENEDDWEYDRESEEICSFQVYGSMKEVSNEVPQKLMDYQTGTD 240

Query: 241 GRPPHVLRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCI 300
           GRPP VLRGCDKELETNEQVPPSSCTPSY+DVGMSYDLCACDEGLENDEREAASCGQSC 
Sbjct: 241 GRPPPVLRGCDKELETNEQVPPSSCTPSYVDVGMSYDLCACDEGLENDEREAASCGQSCT 300

Query: 301 VAGTSRTEIVIDDEEENQLEGSSMNLQKQPGRENLTSGIASEISKVSRWNNGWVPTVEYE 360
           VAGTSRTEI+IDD EENQ EGS MNLQ+QPGR+NLTSGIASEISKVSR +NG VPTVEYE
Sbjct: 301 VAGTSRTEIIIDDGEENQFEGSGMNLQEQPGRKNLTSGIASEISKVSRCSNGLVPTVEYE 360

Query: 361 VIDVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 396
           VIDVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL
Sbjct: 361 VIDVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 401

BLAST of CSPI01G24370 vs. NCBI nr
Match: KAA0060004.1 (Structure-specific endonuclease subunit SLX1 [Cucumis melo var. makuwa] >TYJ97262.1 Structure-specific endonuclease subunit SLX1 [Cucumis melo var. makuwa])

HSP 1 Score: 736.1 bits (1899), Expect = 1.6e-208
Identity = 358/382 (93.72%), Postives = 368/382 (96.34%), Query Frame = 0

Query: 15  DEEKE-DDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHNGEIRCGA 74
           DEEKE DDEEEERGN VNGFFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHNGEIRCGA
Sbjct: 7   DEEKEDDDEEEERGNGVNGFFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHNGEIRCGA 66

Query: 75  WRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLA 134
           WRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLA
Sbjct: 67  WRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLA 126

Query: 135 YTMLTLPAWRGLNITVNYFSTKFIKNAAGCPSLPEHMKVQVSPINELPCYSEGDQDMLEN 194
           YTMLTLPAW GLNITVNYFSTKF+KNAAGCPSLPEHMKVQVSPI+ELPCYSEGDQ +LEN
Sbjct: 127 YTMLTLPAWCGLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPIDELPCYSEGDQGVLEN 186

Query: 195 EGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGRPPHVLRGCDKELETNEQ 254
           E DWEY+RE EEIC F+VYGSMKEVSNEVPQKLMDYQTGTDGRPP VLRGCDKELETNEQ
Sbjct: 187 EDDWEYDRESEEICSFQVYGSMKEVSNEVPQKLMDYQTGTDGRPPPVLRGCDKELETNEQ 246

Query: 255 VPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAGTSRTEIVIDDEEENQL 314
           VPPSSCTPSY+DVGMSYDLCACDEGLENDEREAASCGQSC VAGTSRTEI+IDD EENQ 
Sbjct: 247 VPPSSCTPSYVDVGMSYDLCACDEGLENDEREAASCGQSCTVAGTSRTEIIIDDGEENQF 306

Query: 315 EGSSMNLQKQPGRENLTSGIASEISKVSRWNNGWVPTVEYEVIDVSTPSPDCRTSSHRFK 374
           EGS MNLQ+QPGR+NLTSGIASEISKVSR +NG VPTVEYEVIDVSTPSPDCRTSSHRFK
Sbjct: 307 EGSGMNLQEQPGRKNLTSGIASEISKVSRCSNGLVPTVEYEVIDVSTPSPDCRTSSHRFK 366

Query: 375 RRVTSGKSEMIDLTKSPTFIQL 396
           RRVTSGKSEMIDLTKSPTFIQL
Sbjct: 367 RRVTSGKSEMIDLTKSPTFIQL 388

BLAST of CSPI01G24370 vs. NCBI nr
Match: KGN66284.2 (hypothetical protein Csa_023148 [Cucumis sativus])

HSP 1 Score: 706.1 bits (1821), Expect = 1.8e-199
Identity = 339/344 (98.55%), Postives = 342/344 (99.42%), Query Frame = 0

Query: 52  IGFTVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESL 111
           I  +VNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESL
Sbjct: 19  IPISVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESL 78

Query: 112 AVRSAAATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFIKNAAGCPSLPEHMK 171
           AVRSAAATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKF+KNAAGCPSLPEHMK
Sbjct: 79  AVRSAAATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFMKNAAGCPSLPEHMK 138

Query: 172 VQVSPINELPCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQT 231
           VQVSPINELPCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQT
Sbjct: 139 VQVSPINELPCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQT 198

Query: 232 GTDGRPPHVLRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQ 291
           GTDGRPPHVLRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQ
Sbjct: 199 GTDGRPPHVLRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQ 258

Query: 292 SCIVAGTSRTEIVIDDEEENQLEGSSMNLQKQPGRENLTSGIASEISKVSRWNNGWVPTV 351
           SCIVAGTSRTEIVIDDEEENQLEGSSMNLQ+QPGRENLTSGIASEISKVSRWNNGWVPTV
Sbjct: 259 SCIVAGTSRTEIVIDDEEENQLEGSSMNLQEQPGRENLTSGIASEISKVSRWNNGWVPTV 318

Query: 352 EYEVIDVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 396
           EYEVIDVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL
Sbjct: 319 EYEVIDVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 362

BLAST of CSPI01G24370 vs. NCBI nr
Match: XP_038894793.1 (structure-specific endonuclease subunit SLX1 [Benincasa hispida])

HSP 1 Score: 665.2 bits (1715), Expect = 3.4e-187
Identity = 334/401 (83.29%), Postives = 356/401 (88.78%), Query Frame = 0

Query: 1   MRKRKEKPEICKTM---DEEKEDD---EEEERGNEVNGFFSCYLLASACPRFKGHTYIGF 60
           MRKRKE+PEI KTM   +EEKEDD   EEEE  NEVNGFFSCYLLASACPR+KGHTYIGF
Sbjct: 1   MRKRKERPEISKTMAVAEEEKEDDEDEEEEEGSNEVNGFFSCYLLASACPRYKGHTYIGF 60

Query: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR 120
           TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR
Sbjct: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR 120

Query: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFIKNAAGCPSLPEHMKVQV 180
           SAAATFKSLSGVANKVKLAYTMLTLPAW  LNITVNYFSTK++KNAAGCPSLP+HMKVQV
Sbjct: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPKHMKVQV 180

Query: 181 SPINELPCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTD 240
           SPINELPCYSEGDQ +LENE DWEYNRE EEIC FRVYGSMKEVSNEVPQKLMD+Q  TD
Sbjct: 181 SPINELPCYSEGDQCVLENEDDWEYNRECEEICSFRVYGSMKEVSNEVPQKLMDHQASTD 240

Query: 241 GRPPHVLRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCI 300
           GR P  L GCDKE E NEQVPPSSC PSYID GMSYDL  CDE LE DERE ASC  SCI
Sbjct: 241 GR-PRELHGCDKEFENNEQVPPSSCNPSYIDAGMSYDLHGCDEELEEDEREPASCAPSCI 300

Query: 301 VAGTSRTEIVIDDEEENQLEGSSMNLQKQPGRENLTSGIASEISKVSRWNNGWVPTVEYE 360
           V   SRTEI+IDD +E+Q+EG+ MNL++Q  R+NL+SGIASEISKVSR N   +P VEYE
Sbjct: 301 VTDISRTEIIIDDGDEDQIEGTGMNLEQQLCRKNLSSGIASEISKVSRCNIE-LPPVEYE 360

Query: 361 VIDVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 396
           VIDVSTPSPDCRT+SHR KR+VTSG+SE+IDLTKSPTFIQL
Sbjct: 361 VIDVSTPSPDCRTNSHRVKRKVTSGRSEIIDLTKSPTFIQL 399

BLAST of CSPI01G24370 vs. TAIR 10
Match: AT2G30350.2 (Excinuclease ABC, C subunit, N-terminal )

HSP 1 Score: 266.9 bits (681), Expect = 2.5e-71
Identity = 179/407 (43.98%), Postives = 223/407 (54.79%), Query Frame = 0

Query: 5   KEKPEICKTMDEEKEDDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVNPKRRIRQ 64
           +EK    K +D   ED      G +  GFF+CYLL S  PR KG TYIGFTVNP+RRIRQ
Sbjct: 2   REKRGNRKALDPVGEDG---VTGKDGKGFFACYLLTSLSPRHKGQTYIGFTVNPRRRIRQ 61

Query: 65  HNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLS 124
           HNGEI  GAWRTK+KRPWEMVLCIYGFPTNVSALQFEWAWQHP ES+AVR AAA FKS S
Sbjct: 62  HNGEITSGAWRTKKKRPWEMVLCIYGFPTNVSALQFEWAWQHPRESVAVREAAAAFKSFS 121

Query: 125 GVANKVKLAYTMLTLPAWRGLNITVNYFSTKFIKNAAGCPSLPEHMKVQVSPINELPCYS 184
           GVA+K+KL YTML LPAW  LN+TVNYFS+K+  +    PSLP HMKVQV  + +L  ++
Sbjct: 122 GVASKIKLVYTMLNLPAWNSLNLTVNYFSSKYAHHGGKSPSLPLHMKVQVCAMEDLQYFT 181

Query: 185 E-GDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGR------- 244
           +  D    E+E   E N E ++           + S+ + Q      + +D R       
Sbjct: 182 KVDDSSQPEDEESPEVNEEDDD--------DDDDDSSNLSQPGNSNTSSSDDRHFEKAKE 241

Query: 245 PPHVLRGCDK--------ELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAAS 304
           P  V    D+         L+  E V       S+I VG         E + ND   + +
Sbjct: 242 PVTVFDEEDRLANFSGFGLLDEEETVEDE---VSHITVGSIRATEKEPETVFNDRLASFT 301

Query: 305 CGQSCIVAGTSRTEIVIDDEEENQLEGSSMNLQKQ-PGRENLTSGIASEISKVSRWNNGW 364
           C            EIV +DE  +   GS+  ++K+   R N  +   +E           
Sbjct: 302 C--------FGLVEIV-EDEVSHGTIGSTEAMEKECRKRRNHITSTTTE----------- 361

Query: 365 VPTVEYEVIDVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQ 395
              V+ EVID+ TPSP CR  S   +RRV    SE IDLT SP FI+
Sbjct: 362 ---VDVEVIDLMTPSPSCRAGSSMKRRRV----SEFIDLTMSPNFIE 367

BLAST of CSPI01G24370 vs. TAIR 10
Match: AT2G30350.1 (Excinuclease ABC, C subunit, N-terminal )

HSP 1 Score: 144.8 bits (364), Expect = 1.5e-34
Identity = 116/314 (36.94%), Postives = 154/314 (49.04%), Query Frame = 0

Query: 98  LQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFI 157
           LQFEWAWQHP ES+AVR AAA FKS SGVA+K+KL YTML LPAW  LN+TVNYFS+K+ 
Sbjct: 58  LQFEWAWQHPRESVAVREAAAAFKSFSGVASKIKLVYTMLNLPAWNSLNLTVNYFSSKYA 117

Query: 158 KNAAGCPSLPEHMKVQVSPINELPCYSE-GDQDMLENEGDWEYNREREEICGFRVYGSMK 217
            +    PSLP HMKVQV  + +L  +++  D    E+E   E N E ++           
Sbjct: 118 HHGGKSPSLPLHMKVQVCAMEDLQYFTKVDDSSQPEDEESPEVNEEDDD--------DDD 177

Query: 218 EVSNEVPQKLMDYQTGTDGR-------PPHVLRGCDK--------ELETNEQVPPSSCTP 277
           + S+ + Q      + +D R       P  V    D+         L+  E V       
Sbjct: 178 DDSSNLSQPGNSNTSSSDDRHFEKAKEPVTVFDEEDRLANFSGFGLLDEEETVEDE---V 237

Query: 278 SYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAGTSRTEIVIDDEEENQLEGSSMNLQ 337
           S+I VG         E + ND   + +C            EIV +DE  +   GS+  ++
Sbjct: 238 SHITVGSIRATEKEPETVFNDRLASFTC--------FGLVEIV-EDEVSHGTIGSTEAME 297

Query: 338 KQ-PGRENLTSGIASEISKVSRWNNGWVPTVEYEVIDVSTPSPDCRTSSHRFKRRVTSGK 395
           K+   R N  +   +E              V+ EVID+ TPSP CR  S   +RRV    
Sbjct: 298 KECRKRRNHITSTTTE--------------VDVEVIDLMTPSPSCRAGSSMKRRRV---- 333

BLAST of CSPI01G24370 vs. TAIR 10
Match: AT5G43210.1 (Excinuclease ABC, C subunit, N-terminal )

HSP 1 Score: 57.0 bits (136), Expect = 4.0e-08
Identity = 30/72 (41.67%), Postives = 38/72 (52.78%), Query Frame = 0

Query: 34  FSCYLLASACPRFKGHTYIGFTVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPT 93
           +S YL+ S     K  TY+G T +  RR++QHNGEIR GA  +   RPW     I GF  
Sbjct: 54  WSVYLILSTTEPIK--TYVGITTDFSRRLKQHNGEIRGGAKASSAGRPWLCACIITGFTC 113

Query: 94  NVSALQFEWAWQ 106
              A  FE  W+
Sbjct: 114 LSQASSFESKWK 123

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8BX321.8e-2940.85Structure-specific endonuclease subunit SLX1 OS=Mus musculus OX=10090 GN=Slx1b P... [more]
Q5PQP55.2e-2940.24Structure-specific endonuclease subunit SLX1 OS=Rattus norvegicus OX=10116 GN=Sl... [more]
Q32PI09.9e-2842.07Structure-specific endonuclease subunit SLX1 OS=Bos taurus OX=9913 GN=SLX1A PE=2... [more]
Q0IH864.9e-2738.31Structure-specific endonuclease subunit slx1 OS=Xenopus laevis OX=8355 GN=slx1a ... [more]
A7STV92.4e-2640.94Structure-specific endonuclease subunit SLX1 homolog OS=Nematostella vectensis O... [more]
Match NameE-valueIdentityDescription
A0A0A0M0Y66.5e-23299.24Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis sativus OX=3659 ... [more]
A0A1S3C5749.4e-21592.77Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis melo OX=3656 GN=... [more]
A0A5D3BBR07.7e-20993.72Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis melo var. makuwa... [more]
A0A6J1I6812.2e-16377.31Structure-specific endonuclease subunit SLX1 homolog OS=Cucurbita maxima OX=3661... [more]
A0A6J1HZQ22.2e-16377.31Structure-specific endonuclease subunit SLX1 homolog OS=Cucurbita maxima OX=3661... [more]
Match NameE-valueIdentityDescription
XP_004153269.11.3e-23199.24structure-specific endonuclease subunit SLX1 homolog [Cucumis sativus][more]
XP_008457313.11.9e-21492.77PREDICTED: uncharacterized protein LOC103497035 [Cucumis melo][more]
KAA0060004.11.6e-20893.72Structure-specific endonuclease subunit SLX1 [Cucumis melo var. makuwa] >TYJ9726... [more]
KGN66284.21.8e-19998.55hypothetical protein Csa_023148 [Cucumis sativus][more]
XP_038894793.13.4e-18783.29structure-specific endonuclease subunit SLX1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT2G30350.22.5e-7143.98Excinuclease ABC, C subunit, N-terminal [more]
AT2G30350.11.5e-3436.94Excinuclease ABC, C subunit, N-terminal [more]
AT5G43210.14.0e-0841.67Excinuclease ABC, C subunit, N-terminal [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR035901GIY-YIG endonuclease superfamilyGENE3D3.40.1440.10coord: 32..132
e-value: 6.2E-24
score: 85.9
IPR000305GIY-YIG endonucleasePFAMPF01541GIY-YIGcoord: 37..109
e-value: 2.2E-12
score: 47.1
IPR000305GIY-YIG endonucleasePROSITEPS50164GIY_YIGcoord: 32..114
score: 17.191767
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availablePANTHERPTHR20208:SF10STRUCTURE-SPECIFIC ENDONUCLEASE SUBUNIT SLX1coord: 15..390
NoneNo IPR availablePANTHERPTHR20208STRUCTURE-SPECIFIC ENDONUCLEASE SUBUNIT SLX1coord: 15..390
NoneNo IPR availableCDDcd10455GIY-YIG_SLX1coord: 33..107
e-value: 7.772E-53
score: 168.565
IPR027520Structure-specific endonuclease subunit Slx1HAMAPMF_03100Endonuc_su_Slx1coord: 29..381
score: 23.576988

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G24370.1CSPI01G24370.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000724 double-strand break repair via homologous recombination
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0051569 regulation of histone H3-K4 methylation
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0006281 DNA repair
cellular_component GO:0016593 Cdc73/Paf1 complex
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0033557 Slx1-Slx4 complex
molecular_function GO:0017108 5'-flap endonuclease activity
molecular_function GO:0008821 crossover junction endodeoxyribonuclease activity
molecular_function GO:0000993 RNA polymerase II complex binding