CSPI01G23750 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G23750
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionPhosphomethylpyrimidine synthase
LocationChr1: 19235951 .. 19241531 (+)
RNA-Seq ExpressionCSPI01G23750
SyntenyCSPI01G23750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGGCTCCCTTCTATAAAACCCAATCCTCTTCCATCCTCACTATTATCTTCACTCTTTGCTCGAACCGCGTGTTTAACAGATAAGATTCAACTCGCAAGCATTCGTCGCTAGGTTCTTCCAAACAAAACCCTACATCTTTTCCATTTCCATTTCCATTTCCCCTCCTTAATTCTCTTATTTCTCTATCTTAATCCATTCTAAAACTACATTTCATGCACTCCCTTGTGTATTCTGTTCCATTTTCTGTTATTGTTATTTCGTTTTCTTTGATCAGATCGCTTTGTTGTTGCATGAACTGCTGAGTTCGTTTGATGACTTTGTTTGCGCTTTAGTTTTCATCGTTTGCCGTCGAGATCGTTTGATAGGCGAGTCAAGTGAAAATTCTGTATGATTTATGGATTTTATTGGAGCGTTTCGTGTAAAATCTGTCTTGTTCTTTCTATTATCTGTATTGTAGTGATTTTTTTTTTTTTTTTTTGTGTTGAAGATTTTTTATGATGTAAAGATATTGCCCATTTTAAAGGATTTTCTCCACTGGTTACTAGAGGTCCTAGATTGAGCTTTCATTCGGCTGTATTTTGATGATGCCTTTTGTGTGTTTTTTCTTTTTTCTTTTTAGCCTTTTCGGGCACATGGAGTCTGGTTTTGAACGACATACTAAGATGTTTAAGCTGCTTATTTGCTTTTTTTCTATTTTTGGTATGACAGAGTGGAGTCTGATTTTGAACGACATAAATAGTATGATATTTATGAAGCTAGTTGTGCTTGATTCTGAAAATTGCTTTTGATGCTCAAGAAACCTTTTTTGTTTTTCTTCAATGGTAGGGTTTTAACCATTATTATTGTTATTTTTAAAAAAAATTCTAGATCAAAGATGGCGTCAGTTCATGCTACTATAACACCAGCTGCGGCTGTTGGTAAGAGTGGGAACCGTTCCTCTCCTACGAAATCACTTAATACTGCCTTCTTGCCTGGGTTTGACGTGGTTGGGCGTGTTGCCAGTGCATGCAAGGACTTACACCCTTCATCTATTACCTTAGCTCCTAGAGCCACTTTAACCTTTGATCCCCCGGAAACTAGTACAGAGAAAGCCAAGGACAGGAAGCATACAATTGATCCATCATCTCCTGATTTTCTGCCACTTCCTTCATTTGAACAGTGTTTCCCAAAAAGCACAAAAGAACACACGTGAGTGAATTCACTGGGGTATCATGGTTTTTGTAATTGTTGCAAAACTAGTATATGATGACCATCTGTTTGCAATTATTGATGTGTAATCTGTTATGCAGGGAAGTCGTTCATGAAGAAACTGGGCATGTGCTCAAAGTACCCTTTCGTCGGGTTCATTTATCTGGTGATGAACCAAACTTCGATAATTATGACACCAGTGGTCCTCAAAACATCAACCCCCGTATTGGTAAGACGAGCAGCTTTTGGCTGTGTTCCAAGACTCATTTTTGCTATATTTGTGTTGTAGCAGTGGCACATATTGGTTTTGTTTGTTAGTTCTTAATTGTTCGTGAATTTTATTTCTACTCAAAATAATTTATGTGAAAACTATTTGGAGTCCAGTTGTTGTTGCTGTAAACTGAGTTCTTCTATTTGGACATACATGTCTGTTATTTTAAGTCTGCAGCATAAGAGGGCTACTTTCCCCCCTTAAACTTAGGTTCCAACTAGTGTCAAACTGAAATCTTGTAGATGTGGGCCTATATGACTACGTAGAGAAAAATAAACTGCGGCAAATACAATTGCTTTCTATGAAGGAAACCAAGTCCCTTTGATATTTTATTACACTTTTTGTAATTCAACATCTGTATATGATGCTTTCTGACTTCCCTGATACAACAATGCAAACTAGCATACTTGTTACCTGTCTTTCAAGTTACATTGTTTCTGACAATCAACAATGTTGCACAGGATTGCCCAAACTTCGGAAGGACTGGGTTGATAGGAGGGACAAATTAGGTTCTCCAAGATACACACAGATGTATTATGCCAAGCAAGGAATCATTACTGAGGAAATGTTGTATTGTGCCACTCGTGAGAAGCTTGACCCAGAGTTTGTGAGATCAGAGGTTGCTCGAGGGCGAGCAATCATCCCGTCCAACAAGAATCATTTGGAGTTGGAGCCCATGATTGTGGGTAGAAATTTCTTGGTCAAAGTAAATGCAAATATTGGAAACTCCGCTGTAGCAAGTTCTATTGAAGAAGAAGTTTATAAAGTCCAATGGGCGACCATGTGGGGAGCTGATACTGTGATGGATCTCTCTACTGGTCGTCACATACATGAGACCCGTGAGTGGATATTGCGTAATTCATCTGTACCTGTAGGCACTGTTCCCATATATCAGGCACTTGAGAAAGTGAACGGCATAGCTGAAAATCTCACCTGGGAAATTTTCAGGGAAACACTGATTGAACAAGCTGAGCAGGGTGTAGACTATTTTACCATTCATGCTGGGGTCTTACTTCGATACATCCCTCTAACAGCAAAAAGAATGACAGGAATTGTATCACGTGGAGGATCTATTCATGCAAAGTGGTGTTTGGCTCATCATAAGGAAAATTTCGCTTATGAACACTGGGATGACATACTTGACATCTGTAATCAGTATGATATATCCCTATCGATTGGTGATGGGCTGAGACCTGGTTCTATTTATGATGCCAACGACACTGCTCAATTTGCAGAGCTCCTAACTCAGGGCGAACTGACTCGTAGAGCATGGGAGAAAGACGTGCAGGTAATGAATATAAATCTCTCTTGCTTACATATATGCATGTGTTCACAATCACACCTATGCATTTGAGAGCAATCCTTTTTACTCCTAGTTATATGCATTTGAAAGAACATCTCACTGTTGTAACATTTGTAAGAACCACTCTAAGGCTTACCTAGTACAGTCAGTTTCATGCATGTCTTCAAACATTTGTGGCTGAATTGTCGGGAATTTAGGAGCCTCTCAAGCAGTTTCTTATGATTTCCTCACCCAATAACACTTTTTTACGCTGGTTTGGTGGAGAGCTTCAATTTACAATACAGGATACGTCTCTTAAATAGCAGATTATTTTCCCACAATTAGTTTTATGGGAGTGTATGTTAAAATGTGGAACTTACATTTTCATTTTTGGAAGCAGGGAATGTTATATCTTCCATGTTACTAACTAGTGACCACATACTTGATTTTCAGGTAATGAATGAAGGACCTGGGCATATCCCAATGCATAAGATCCCTGAAAACATGCAAAAACAGCTTGAGTGGTGTAATGAAGCACCTTTCTACACTCTTGGTCCTTTGACTACAGATGTAGCTCCTGGATACGACCACATTACCTCTGCCATTGGAGCTGCCAATATTGGTGCTCTTGGCACGGCCCTTCTCTGTTATGTTACACCGAAAGAGCATCTAGGTTTGCCAAATCGAGATGATGTGAAAGCTGGAGTAATAGCATATAAGATAGCTGCCCATGCAGCTGATCTAGCCAAAGGTCATCCACATGCTCAATCATGGGATGATGCACTGAGCAAGGCGAGGTTTGAGTTCCGGTGGATGGATCAATTTGCTTTGTCATTGGACCCTATGACTGCCATGTCATTTCATGATGAAACCTTGCCATCAGAAGGTGCCAAGGTGGCTCACTTCTGTTCCATGTGTGGACCCAAGTTCTGCTCCATGAAGATAACCGAGGATGTGCGTAAGTATGCTGAAGAACACGGGTACGGGAGTGCAGAGGAAGCTCTGCAGGAAGGGATGGATGCTATGAGTGCTGAGTTCTTGGCTGCAAAGAAAACCATTAGTGGTGAACAACATGGTGAAACTGGTGGGGAAATCTACTTGCCTGCAAGCTACGTGGACTCCCAGAAGAGGTGAAGGTTAGTAATCACAGAAACTTTTGGCTTTTGTTTTAATGTTCGGTTTTCTTATCATAGAACGAAATTTGAATAAATATTTCAATTACAATCTTTCAATTCTATTCTTGAAAAGGACTTGTTTTCCACAAGAAGATGCAATATAAGTGGAACACCTTGGCATGACGACCTCTGCTCGACATAGTAATCGATTATTATTTGATCTTTAATAGGTTGGTCGCATGGTAATAAATAGTTTGTCACACCTAGATGAGGATGCACTATGTAGGAAATGCTTGGAATGACTTTGAAGTGCAAGAGTATTCATGCCCTAAAAAAATTATTTTAAGTATTTGGAAAGTCATTACGAGTGATCCCTTGGTAAGGCTATGGAGTAAGGGTTTTGCCATCCTTTCCTCAAAACTTATCTTATGGAATTCTAACTTTGAAGGATCATTGCCCGGACTAAATTTGGCTAAAACATGGGGTTTTGTGATGTTATTTTGAAAGCAGAATGCGGTCTAATATTGTTCCTCTTTATGATAAGTTCTAAGAAGAGCAAGGAGAAATCATCTTTTAGCAATTGCTTCGTTACTTGTGTTGGTTCTTATAGGTATAATACCAATGCAGGACCATTCGATTCGTGAAGGAGAAAGGGTTGGAAGTGCAAGAGACTTATCGTCGATGATTGGCCGACCAGAACAGAAAATTGATACCTGCTCGAGTCTCCAGTTTCAGGCCATGGAAGAGTACAAAGTTTAGCTGCTCTGTTCTTTTTTTATTGGCTGTATTTATGTTCGTCTGCTTTGTTTCTCTATCTGTTGGAACTAAGAGGAAGTTATTGGTTTGCTTGATGTTTTGACCTTGTCTCTTCCATCATTTTTTCTTTTAAATTCGTATATTAACGTATGAAACGCACCAGGGGTGCCTCGACCAAATGTAGCTGCTCGATCGGCAGTTAACTGGTCAGGCTGAGATAGTCCCTTTGAACCTGAACAGGATAATGCCTGCGTAGGGAGCGTGCTTTTCTGTTTTTTTGTATACAGGAATGGCGCTGCTATGATCGTGACTATCGTTTTGGTCGAGGATTCATTCGAGCCTTCATTTCTTAAGAAAGGGAACAACTGAAGGCGGGAGATTCTAATCTCATTTACCAAAACATTTAAAAACTCCTAAACTGAAACCCTTTGGCATTTTACCCTCTGGCAAAGTCAATAAGAATATGAAAGTGGTAAATAAAAACTTTTAAAAATTGAAAGAAGAAAAGAAAAGAAAAATCAACTTTGAGTGTTCATTTTGTGTAAAAGCAAAAAGATCATGATTTTTGGCTATATAAAAGAAAACCAGTTTCTTGTTTTTAACGAACCATTAGGGTTCCTATAGTATAAAGATGAATGTAAATTTAAACACTAAATTTTGATTTTGAACTAAGACATATTTTGGATACGATCCAAGGATCTCGATATGTCCAAAAGCACATACTTCAGTATCCTTACCAAATGTTGAAAGTATGGTTAGGCTGAAACCACAAAAGTTCTATACTAAAATTAAATACTTGAGAATGAAGCACGGAAAAACTTGAAATTTTAAGTGTGTTAAATCAGTTAGATAAAAACGGATGGATTGTCCTTTATTCATTGGATAAATAAAACCAACTTCGATTGATAATCACTCCTCATTTCTATTTCTCACCTAGAAAAAATACTTTTAAGCAAATAAGAATAATCT

mRNA sequence

CGGCTCCCTTCTATAAAACCCAATCCTCTTCCATCCTCACTATTATCTTCACTCTTTGCTCGAACCGCGTGTTTAACAGATAAGATTCAACTCGCAAGCATTCGTCGCTAGATCAAAGATGGCGTCAGTTCATGCTACTATAACACCAGCTGCGGCTGTTGGTAAGAGTGGGAACCGTTCCTCTCCTACGAAATCACTTAATACTGCCTTCTTGCCTGGGTTTGACGTGGTTGGGCGTGTTGCCAGTGCATGCAAGGACTTACACCCTTCATCTATTACCTTAGCTCCTAGAGCCACTTTAACCTTTGATCCCCCGGAAACTAGTACAGAGAAAGCCAAGGACAGGAAGCATACAATTGATCCATCATCTCCTGATTTTCTGCCACTTCCTTCATTTGAACAGTGTTTCCCAAAAAGCACAAAAGAACACACGGAAGTCGTTCATGAAGAAACTGGGCATGTGCTCAAAGTACCCTTTCGTCGGGTTCATTTATCTGGTGATGAACCAAACTTCGATAATTATGACACCAGTGGTCCTCAAAACATCAACCCCCGTATTGGATTGCCCAAACTTCGGAAGGACTGGGTTGATAGGAGGGACAAATTAGGTTCTCCAAGATACACACAGATGTATTATGCCAAGCAAGGAATCATTACTGAGGAAATGTTGTATTGTGCCACTCGTGAGAAGCTTGACCCAGAGTTTGTGAGATCAGAGGTTGCTCGAGGGCGAGCAATCATCCCGTCCAACAAGAATCATTTGGAGTTGGAGCCCATGATTGTGGGTAGAAATTTCTTGGTCAAAGTAAATGCAAATATTGGAAACTCCGCTGTAGCAAGTTCTATTGAAGAAGAAGTTTATAAAGTCCAATGGGCGACCATGTGGGGAGCTGATACTGTGATGGATCTCTCTACTGGTCGTCACATACATGAGACCCGTGAGTGGATATTGCGTAATTCATCTGTACCTGTAGGCACTGTTCCCATATATCAGGCACTTGAGAAAGTGAACGGCATAGCTGAAAATCTCACCTGGGAAATTTTCAGGGAAACACTGATTGAACAAGCTGAGCAGGGTGTAGACTATTTTACCATTCATGCTGGGGTCTTACTTCGATACATCCCTCTAACAGCAAAAAGAATGACAGGAATTGTATCACGTGGAGGATCTATTCATGCAAAGTGGTGTTTGGCTCATCATAAGGAAAATTTCGCTTATGAACACTGGGATGACATACTTGACATCTGTAATCAGTATGATATATCCCTATCGATTGGTGATGGGCTGAGACCTGGTTCTATTTATGATGCCAACGACACTGCTCAATTTGCAGAGCTCCTAACTCAGGGCGAACTGACTCGTAGAGCATGGGAGAAAGACGTGCAGGTAATGAATGAAGGACCTGGGCATATCCCAATGCATAAGATCCCTGAAAACATGCAAAAACAGCTTGAGTGGTGTAATGAAGCACCTTTCTACACTCTTGGTCCTTTGACTACAGATGTAGCTCCTGGATACGACCACATTACCTCTGCCATTGGAGCTGCCAATATTGGTGCTCTTGGCACGGCCCTTCTCTGTTATGTTACACCGAAAGAGCATCTAGGTTTGCCAAATCGAGATGATGTGAAAGCTGGAGTAATAGCATATAAGATAGCTGCCCATGCAGCTGATCTAGCCAAAGGTCATCCACATGCTCAATCATGGGATGATGCACTGAGCAAGGCGAGGTTTGAGTTCCGGTGGATGGATCAATTTGCTTTGTCATTGGACCCTATGACTGCCATGTCATTTCATGATGAAACCTTGCCATCAGAAGGTGCCAAGGTGGCTCACTTCTGTTCCATGTGTGGACCCAAGTTCTGCTCCATGAAGATAACCGAGGATGTGCGTAAGTATGCTGAAGAACACGGGTACGGGAGTGCAGAGGAAGCTCTGCAGGAAGGGATGGATGCTATGAGTGCTGAGTTCTTGGCTGCAAAGAAAACCATTAGTGGTGAACAACATGGTGAAACTGGTGGGGAAATCTACTTGCCTGCAAGCTACGTGGACTCCCAGAAGAGGTGAAGGACCATTCGATTCGTGAAGGAGAAAGGGTTGGAAGTGCAAGAGACTTATCGTCGATGATTGGCCGACCAGAACAGAAAATTGATACCTGCTCGAGTCTCCAGTTTCAGGCCATGGAAGAGTACAAAGTTTAGCTGCTCTGTTCTTTTTTTATTGGCTGTATTTATGTTCGTCTGCTTTGTTTCTCTATCTGTTGGAACTAAGAGGAAGTTATTGGTTTGCTTGATGTTTTGACCTTGTCTCTTCCATCATTTTTTCTTTTAAATTCGTATATTAACGTATGAAACGCACCAGGGGTGCCTCGACCAAATGTAGCTGCTCGATCGGCAGTTAACTGGTCAGGCTGAGATAGTCCCTTTGAACCTGAACAGGATAATGCCTGCGTAGGGAGCGTGCTTTTCTGTTTTTTTGTATACAGGAATGGCGCTGCTATGATCGTGACTATCGTTTTGGTCGAGGATTCATTCGAGCCTTCATTTCTTAAGAAAGGGAACAACTGAAGGCGGGAGATTCTAATCTCATTTACCAAAACATTTAAAAACTCCTAAACTGAAACCCTTTGGCATTTTACCCTCTGGCAAAGTCAATAAGAATATGAAAGTGGTAAATAAAAACTTTTAAAAATTGAAAGAAGAAAAGAAAAGAAAAATCAACTTTGAGTGTTCATTTTGTGTAAAAGCAAAAAGATCATGATTTTTGGCTATATAAAAGAAAACCAGTTTCTTGTTTTTAACGAACCATTAGGGTTCCTATAGTATAAAGATGAATGTAAATTTAAACACTAAATTTTGATTTTGAACTAAGACATATTTTGGATACGATCCAAGGATCTCGATATGTCCAAAAGCACATACTTCAGTATCCTTACCAAATGTTGAAAGTATGGTTAGGCTGAAACCACAAAAGTTCTATACTAAAATTAAATACTTGAGAATGAAGCACGGAAAAACTTGAAATTTTAAGTGTGTTAAATCAGTTAGATAAAAACGGATGGATTGTCCTTTATTCATTGGATAAATAAAACCAACTTCGATTGATAATCACTCCTCATTTCTATTTCTCACCTAGAAAAAATACTTTTAAGCAAATAAGAATAATCT

Coding sequence (CDS)

ATGGCGTCAGTTCATGCTACTATAACACCAGCTGCGGCTGTTGGTAAGAGTGGGAACCGTTCCTCTCCTACGAAATCACTTAATACTGCCTTCTTGCCTGGGTTTGACGTGGTTGGGCGTGTTGCCAGTGCATGCAAGGACTTACACCCTTCATCTATTACCTTAGCTCCTAGAGCCACTTTAACCTTTGATCCCCCGGAAACTAGTACAGAGAAAGCCAAGGACAGGAAGCATACAATTGATCCATCATCTCCTGATTTTCTGCCACTTCCTTCATTTGAACAGTGTTTCCCAAAAAGCACAAAAGAACACACGGAAGTCGTTCATGAAGAAACTGGGCATGTGCTCAAAGTACCCTTTCGTCGGGTTCATTTATCTGGTGATGAACCAAACTTCGATAATTATGACACCAGTGGTCCTCAAAACATCAACCCCCGTATTGGATTGCCCAAACTTCGGAAGGACTGGGTTGATAGGAGGGACAAATTAGGTTCTCCAAGATACACACAGATGTATTATGCCAAGCAAGGAATCATTACTGAGGAAATGTTGTATTGTGCCACTCGTGAGAAGCTTGACCCAGAGTTTGTGAGATCAGAGGTTGCTCGAGGGCGAGCAATCATCCCGTCCAACAAGAATCATTTGGAGTTGGAGCCCATGATTGTGGGTAGAAATTTCTTGGTCAAAGTAAATGCAAATATTGGAAACTCCGCTGTAGCAAGTTCTATTGAAGAAGAAGTTTATAAAGTCCAATGGGCGACCATGTGGGGAGCTGATACTGTGATGGATCTCTCTACTGGTCGTCACATACATGAGACCCGTGAGTGGATATTGCGTAATTCATCTGTACCTGTAGGCACTGTTCCCATATATCAGGCACTTGAGAAAGTGAACGGCATAGCTGAAAATCTCACCTGGGAAATTTTCAGGGAAACACTGATTGAACAAGCTGAGCAGGGTGTAGACTATTTTACCATTCATGCTGGGGTCTTACTTCGATACATCCCTCTAACAGCAAAAAGAATGACAGGAATTGTATCACGTGGAGGATCTATTCATGCAAAGTGGTGTTTGGCTCATCATAAGGAAAATTTCGCTTATGAACACTGGGATGACATACTTGACATCTGTAATCAGTATGATATATCCCTATCGATTGGTGATGGGCTGAGACCTGGTTCTATTTATGATGCCAACGACACTGCTCAATTTGCAGAGCTCCTAACTCAGGGCGAACTGACTCGTAGAGCATGGGAGAAAGACGTGCAGGTAATGAATGAAGGACCTGGGCATATCCCAATGCATAAGATCCCTGAAAACATGCAAAAACAGCTTGAGTGGTGTAATGAAGCACCTTTCTACACTCTTGGTCCTTTGACTACAGATGTAGCTCCTGGATACGACCACATTACCTCTGCCATTGGAGCTGCCAATATTGGTGCTCTTGGCACGGCCCTTCTCTGTTATGTTACACCGAAAGAGCATCTAGGTTTGCCAAATCGAGATGATGTGAAAGCTGGAGTAATAGCATATAAGATAGCTGCCCATGCAGCTGATCTAGCCAAAGGTCATCCACATGCTCAATCATGGGATGATGCACTGAGCAAGGCGAGGTTTGAGTTCCGGTGGATGGATCAATTTGCTTTGTCATTGGACCCTATGACTGCCATGTCATTTCATGATGAAACCTTGCCATCAGAAGGTGCCAAGGTGGCTCACTTCTGTTCCATGTGTGGACCCAAGTTCTGCTCCATGAAGATAACCGAGGATGTGCGTAAGTATGCTGAAGAACACGGGTACGGGAGTGCAGAGGAAGCTCTGCAGGAAGGGATGGATGCTATGAGTGCTGAGTTCTTGGCTGCAAAGAAAACCATTAGTGGTGAACAACATGGTGAAACTGGTGGGGAAATCTACTTGCCTGCAAGCTACGTGGACTCCCAGAAGAGGTGA

Protein sequence

MASVHATITPAAAVGKSGNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRATLTFDPPETSTEKAKDRKHTIDPSSPDFLPLPSFEQCFPKSTKEHTEVVHEETGHVLKVPFRRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGSPRYTQMYYAKQGIITEEMLYCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGIAENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGSAEEALQEGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR*
Homology
BLAST of CSPI01G23750 vs. ExPASy Swiss-Prot
Match: O82392 (Phosphomethylpyrimidine synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THIC PE=1 SV=1)

HSP 1 Score: 1163.7 bits (3009), Expect = 0.0e+00
Identity = 559/648 (86.27%), Postives = 599/648 (92.44%), Query Frame = 0

Query: 2   ASVHATITPAAAVGKSGNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRATL 61
           ASVH T+   + V  + N S+  K  N++ LPGFDVV + A+          T   RATL
Sbjct: 3   ASVHCTL--MSVVCNNKNHSARPKLPNSSLLPGFDVVVQAAAT----RFKKETTTTRATL 62

Query: 62  TFDPPETSTEKAKDRKHTIDPSSPDFLPLPSFEQCFPKSTKEHTEVVHEETGHVLKVPFR 121
           TFDPP T++E+AK RKHTIDPSSPDF P+PSFE+CFPKSTKEH EVVHEE+GHVLKVPFR
Sbjct: 63  TFDPPTTNSERAKQRKHTIDPSSPDFQPIPSFEECFPKSTKEHKEVVHEESGHVLKVPFR 122

Query: 122 RVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGSPRYTQMYYAKQGIITE 181
           RVHLSG EP FDNYDTSGPQN+N  IGL KLRK+W+DRR+KLG+PRYTQMYYAKQGIITE
Sbjct: 123 RVHLSGGEPAFDNYDTSGPQNVNAHIGLAKLRKEWIDRREKLGTPRYTQMYYAKQGIITE 182

Query: 182 EMLYCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVAS 241
           EMLYCATREKLDPEFVRSEVARGRAIIPSNK HLELEPMIVGR FLVKVNANIGNSAVAS
Sbjct: 183 EMLYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVAS 242

Query: 242 SIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGIA 301
           SIEEEVYKVQWATMWGADT+MDLSTGRHIHETREWILRNS+VPVGTVPIYQALEKV+GIA
Sbjct: 243 SIEEEVYKVQWATMWGADTIMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVDGIA 302

Query: 302 ENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHH 361
           ENL WE+FRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKR+TGIVSRGGSIHAKWCLA+H
Sbjct: 303 ENLNWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRLTGIVSRGGSIHAKWCLAYH 362

Query: 362 KENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKD 421
           KENFAYEHWDDILDICNQYD++LSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKD
Sbjct: 363 KENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKD 422

Query: 422 VQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGA 481
           VQVMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTD+APGYDHITSAIGAANIGA
Sbjct: 423 VQVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDIAPGYDHITSAIGAANIGA 482

Query: 482 LGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEF 541
           LGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK HPHAQ+WDDALSKARFEF
Sbjct: 483 LGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKQHPHAQAWDDALSKARFEF 542

Query: 542 RWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYG 601
           RWMDQFALSLDPMTAMSFHDETLP++GAKVAHFCSMCGPKFCSMKITED+RKYAEE+GYG
Sbjct: 543 RWMDQFALSLDPMTAMSFHDETLPADGAKVAHFCSMCGPKFCSMKITEDIRKYAEENGYG 602

Query: 602 SAEEALQEGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 650
           SAEEA+++GMDAMS EF  AKKTISGEQHGE GGEIYLP SYV + ++
Sbjct: 603 SAEEAIRQGMDAMSEEFNIAKKTISGEQHGEVGGEIYLPESYVKAAQK 644

BLAST of CSPI01G23750 vs. ExPASy Swiss-Prot
Match: Q9KBJ4 (Phosphomethylpyrimidine synthase OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=thiC PE=3 SV=1)

HSP 1 Score: 731.1 bits (1886), Expect = 1.1e-209
Identity = 371/583 (63.64%), Postives = 430/583 (73.76%), Query Frame = 0

Query: 92  SFEQCFPKSTKEHTEVVHEETGHVLKVPFRRVHL------SGDEPN--FDNYDTSGP--- 151
           S +  FP S K +      +    ++VPFR +        SG+  N     YD+SGP   
Sbjct: 12  SIQSSFPNSQKRYVTGSRSD----IRVPFREISQHPTVTDSGEAENEPILVYDSSGPYTD 71

Query: 152 --QNINPRIGLPKLRKDWVDRR------------------DKLGSPR------------- 211
               +N   GLP +RK W+  R                   K   PR             
Sbjct: 72  ESYEVNIEKGLPAIRKSWILERGDCEKYEGRDVRPEDNGFTKDDDPRAAREVFPRTSSHK 131

Query: 212 ---------YTQMYYAKQGIITEEMLYCATREKLDPEFVRSEVARGRAIIPSNKNHLELE 271
                     TQM+YAK+GI+T EM + A RE + PEFVR E+ARGRAI+PSN NH E E
Sbjct: 132 PLRAKKGANVTQMHYAKKGIVTPEMEFVAIRENVSPEFVRDELARGRAILPSNINHPESE 191

Query: 272 PMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWIL 331
           PMI+GRNF VK+NANIGNSAV+SSI EEV K+ WAT WGADT+MDLSTG++IH TREWI+
Sbjct: 192 PMIIGRNFHVKINANIGNSAVSSSIAEEVEKMTWATRWGADTIMDLSTGKNIHTTREWII 251

Query: 332 RNSSVPVGTVPIYQALEKVNGIAENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLT 391
           RNS VPVGTVPIYQALEKVNG+AE+LTWE++R+TLIEQAEQGVDYFTIHAGVLLRYIPLT
Sbjct: 252 RNSPVPVGTVPIYQALEKVNGVAEDLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYIPLT 311

Query: 392 AKRMTGIVSRGGSIHAKWCLAHHKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDA 451
           AKR+TGIVSRGGSI A+WCL HHKENF Y H+++I +I   YDI+ S+GDGLRPGSI DA
Sbjct: 312 AKRVTGIVSRGGSIMAQWCLYHHKENFLYTHFEEICEIMKTYDIAFSLGDGLRPGSIADA 371

Query: 452 NDTAQFAELLTQGELTRRAWEKDVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGP 511
           ND AQFAEL T GELT+ AWE DVQVM EGPGH+PMH+I ENM KQL+ C EAPFYTLGP
Sbjct: 372 NDEAQFAELETLGELTKIAWEHDVQVMVEGPGHVPMHQIKENMDKQLDICQEAPFYTLGP 431

Query: 512 LTTDVAPGYDHITSAIGAANIGALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAA 571
           LTTD+APGYDHITSAIGAA IG  GTA+LCYVTPKEHLGLPN+DDV+ GVI YKIAAHAA
Sbjct: 432 LTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAAHAA 491

Query: 572 DLAKGHPHAQSWDDALSKARFEFRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMC 622
           DLAKGHP AQ  DDALSKARFEFRW DQF LSLDP  AM FHDETLP+EGAK AHFCSMC
Sbjct: 492 DLAKGHPGAQKRDDALSKARFEFRWRDQFNLSLDPERAMEFHDETLPAEGAKTAHFCSMC 551

BLAST of CSPI01G23750 vs. ExPASy Swiss-Prot
Match: A7GUZ5 (Phosphomethylpyrimidine synthase OS=Bacillus cytotoxicus (strain DSM 22905 / CIP 110041 / 391-98 / NVH 391-98) OX=315749 GN=thiC PE=3 SV=1)

HSP 1 Score: 720.7 bits (1859), Expect = 1.5e-206
Identity = 370/577 (64.12%), Postives = 430/577 (74.52%), Query Frame = 0

Query: 92  SFEQCFPKSTKEHTEVVH-EETGHVLKVPFRRVHLSGDE--PN--FDNYDTSGP-----Q 151
           S EQ   KS+   ++ V+ E +   +KVP R + LS     PN     YDTSGP      
Sbjct: 6   SAEQIELKSSLPGSKKVYVEGSREGMKVPMREIELSDTNGVPNSPIRVYDTSGPYTDPEY 65

Query: 152 NINPRIGLPKLRKDWV----------------------------------DR---RDKLG 211
            +    G+P  R++W+                                  DR   R K G
Sbjct: 66  KVELEKGIPTPRRNWIIERGDVEEYEGREIKPEDDGVKAASNHTPVFPQMDRKPLRAKKG 125

Query: 212 SPRYTQMYYAKQGIITEEMLYCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGR 271
           +   TQM+YA++GIIT EM Y A RE ++PEFVR E+A GRAI+P+N NH E EPMI+GR
Sbjct: 126 A-NVTQMHYARKGIITSEMEYVAIREGVEPEFVRKEIAEGRAILPANINHPEAEPMIIGR 185

Query: 272 NFLVKVNANIGNSAVASSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVP 331
           NF VKVNANIGNSAV+SSI EEV K+ WAT WGADT+MDLSTG++IH TREWI+RN+ VP
Sbjct: 186 NFHVKVNANIGNSAVSSSIAEEVEKMTWATRWGADTIMDLSTGKNIHTTREWIIRNAPVP 245

Query: 332 VGTVPIYQALEKVNGIAENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTG 391
           VGTVPIYQALEKV GIAENLTWE++R+TLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTG
Sbjct: 246 VGTVPIYQALEKVQGIAENLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTG 305

Query: 392 IVSRGGSIHAKWCLAHHKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQF 451
           IVSRGGSI A+WCL HHKENF Y H+++I +I  QYD+S S+GDGLRPGSI DAND AQF
Sbjct: 306 IVSRGGSIMAQWCLYHHKENFLYTHFEEICEIMKQYDVSFSLGDGLRPGSIADANDEAQF 365

Query: 452 AELLTQGELTRRAWEKDVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVA 511
           AEL T GELT+ AW+ DVQVM EGPGH+PMH I ENM+K+++ C  APFYTLGPLTTD+A
Sbjct: 366 AELETLGELTKIAWKHDVQVMIEGPGHVPMHLIKENMEKEIDICQGAPFYTLGPLTTDIA 425

Query: 512 PGYDHITSAIGAANIGALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGH 571
           PGYDHITSAIGAA IG  GTA+LCYVTPKEHLGLPN+DDV+ GVI YKIAAHAADLAKGH
Sbjct: 426 PGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAAHAADLAKGH 485

Query: 572 PHAQSWDDALSKARFEFRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCS 622
             AQ  DDALSKARFEFRW DQF LSLDP  AM FHDETLP+EGAK AHFCSMCGPKFCS
Sbjct: 486 KTAQQRDDALSKARFEFRWRDQFNLSLDPERAMEFHDETLPAEGAKTAHFCSMCGPKFCS 545

BLAST of CSPI01G23750 vs. ExPASy Swiss-Prot
Match: A4VR38 (Phosphomethylpyrimidine synthase OS=Pseudomonas stutzeri (strain A1501) OX=379731 GN=thiC PE=3 SV=1)

HSP 1 Score: 720.7 bits (1859), Expect = 1.5e-206
Identity = 369/600 (61.50%), Postives = 427/600 (71.17%), Query Frame = 0

Query: 95  QCFPKSTKEHTEVVHEETGHVLKVPFRRVHLSGDEPNFDN--------YDTSGPQ----- 154
           Q FP+S K + +    +    ++VP R + L     +F          YDTSGP      
Sbjct: 21  QPFPRSQKIYVQGSRPD----IRVPMREISLDVTPTDFGGEINAPVTVYDTSGPYTDPNV 80

Query: 155 NINPRIGLPKLRKDWV-DRRD-------------------KLGSPRY------------- 214
            I+ R GL  +R  W+ DR D                   +L + R+             
Sbjct: 81  TIDVRKGLADVRSAWIEDRGDTEKLPGLTSEFGQRRLNDAELSAMRFAHVRNPRRAKAEH 140

Query: 215 --TQMYYAKQGIITEEMLYCATRE----------------------------KLDPEFVR 274
             +QM+YAK+GIIT EM Y A RE                            ++ PEFVR
Sbjct: 141 NVSQMHYAKKGIITPEMEYVAIRENMKLAEAREAGLLVEQHAGQSFGAAIPKEITPEFVR 200

Query: 275 SEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWATMWGA 334
           SEVARGRAIIP+N NH ELEPMI+GRNFLVK+N NIGNSA+ SSIEEEV K+ W   WG+
Sbjct: 201 SEVARGRAIIPANINHTELEPMIIGRNFLVKINGNIGNSALGSSIEEEVAKLTWGIRWGS 260

Query: 335 DTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGIAENLTWEIFRETLIEQAE 394
           DTVMDLSTG+HIHETREWI+RNS VP+GTVPIYQALEKVNG+AE+LTWE+FR+TLIEQAE
Sbjct: 261 DTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGVAEDLTWELFRDTLIEQAE 320

Query: 395 QGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKENFAYEHWDDILDICN 454
           QGVDYFTIHAGVLLRY+PLTAKR+TGIVSRGGSI AKWCLAHHKENF Y H+++I +I  
Sbjct: 321 QGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKENFLYTHFEEICEIMK 380

Query: 455 QYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQVMNEGPGHIPMHKIP 514
            YD+S S+GDGLRPGSI DAND AQF EL T GELT+ AW+ DVQ M EGPGH+PMH I 
Sbjct: 381 AYDVSFSLGDGLRPGSIADANDAAQFGELETLGELTKIAWKHDVQCMIEGPGHVPMHMIK 440

Query: 515 ENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALGTALLCYVTPKEHLGL 574
           ENM KQLE C+EAPFYTLGPLTTD+APGYDHITS IGAA IG  G A+LCYVTPKEHLGL
Sbjct: 441 ENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTPKEHLGL 500

Query: 575 PNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFRWMDQFALSLDPMTAMS 619
           PN+DDVK G+I YKIAAHAADLAKGHP AQ  D+ALSKARFEFRW DQF L LDP TA +
Sbjct: 501 PNKDDVKTGIITYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLGLDPDTARA 560

BLAST of CSPI01G23750 vs. ExPASy Swiss-Prot
Match: A6VD86 (Phosphomethylpyrimidine synthase OS=Pseudomonas aeruginosa (strain PA7) OX=381754 GN=thiC PE=3 SV=1)

HSP 1 Score: 719.2 bits (1855), Expect = 4.3e-206
Identity = 368/600 (61.33%), Postives = 421/600 (70.17%), Query Frame = 0

Query: 95  QCFPKSTKEHTEVVHEETGHVLKVPFRRVHLSGDEPNFDN--------YDTSGPQ----- 154
           Q FP S K +      +    ++VP R + L+     F          YDTSGP      
Sbjct: 21  QPFPNSRKVYLTGSRPD----IRVPVREISLADTPTAFGGEKNPPVFVYDTSGPYTDPEV 80

Query: 155 NINPRIGLPKLRKDWVDRR----------DKLGSPR------------------------ 214
            I+ R GLP +R  W+D R           + G  R                        
Sbjct: 81  RIDLRKGLPDVRSRWIDERGDTEILPGLTSEFGQARLADASLDALRFAHVRTPRRAKPGA 140

Query: 215 -YTQMYYAKQGIITEEMLYCATRE----------------------------KLDPEFVR 274
             +QM+YAK+GIIT EM Y A RE                            ++ PEFVR
Sbjct: 141 NVSQMHYAKKGIITPEMEYIAIRENMKLQEARAAGLLDQQHPGHSFGANIPKEITPEFVR 200

Query: 275 SEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWATMWGA 334
            EVARGRAIIP+N NH ELEPMI+GRNFLVK+N NIGNSA+ SSIEEEV K+ W   WGA
Sbjct: 201 EEVARGRAIIPANINHTELEPMIIGRNFLVKINGNIGNSALGSSIEEEVEKLTWGIRWGA 260

Query: 335 DTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGIAENLTWEIFRETLIEQAE 394
           DTVMDLSTG+HIHETREWILRNS VP+GTVPIYQALEKVNG+AE+LTWEIFR+TLIEQAE
Sbjct: 261 DTVMDLSTGKHIHETREWILRNSPVPIGTVPIYQALEKVNGVAEDLTWEIFRDTLIEQAE 320

Query: 395 QGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKENFAYEHWDDILDICN 454
           QGVDYFTIHAGVLLRY+PLTAKR+TGIVSRGGSI AKWCLAHHKENF Y H+++I +I  
Sbjct: 321 QGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKENFLYTHFEEICEIMK 380

Query: 455 QYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQVMNEGPGHIPMHKIP 514
            YD+S S+GDGLRPGS+ DAND AQF EL T GELT+ AW+ DVQVM EGPGH+PM  I 
Sbjct: 381 AYDVSFSLGDGLRPGSVADANDAAQFGELETLGELTKIAWKHDVQVMIEGPGHVPMQLIK 440

Query: 515 ENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALGTALLCYVTPKEHLGL 574
           ENM KQLE C+EAPFYTLGPLTTD+APGYDHITS IGAA IG  G A+LCYVTPKEHLGL
Sbjct: 441 ENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTPKEHLGL 500

Query: 575 PNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFRWMDQFALSLDPMTAMS 619
           PN+DDVK G+I YKIAAHAADLAKGHP AQ  D+ALSKARFEFRW DQF L LDP TA +
Sbjct: 501 PNKDDVKTGIITYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLGLDPDTARA 560

BLAST of CSPI01G23750 vs. ExPASy TrEMBL
Match: A0A0A0LXK6 (Catalytic OS=Cucumis sativus OX=3659 GN=Csa_1G528590 PE=3 SV=1)

HSP 1 Score: 1338.6 bits (3463), Expect = 0.0e+00
Identity = 649/649 (100.00%), Postives = 649/649 (100.00%), Query Frame = 0

Query: 1   MASVHATITPAAAVGKSGNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRAT 60
           MASVHATITPAAAVGKSGNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRAT
Sbjct: 1   MASVHATITPAAAVGKSGNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRAT 60

Query: 61  LTFDPPETSTEKAKDRKHTIDPSSPDFLPLPSFEQCFPKSTKEHTEVVHEETGHVLKVPF 120
           LTFDPPETSTEKAKDRKHTIDPSSPDFLPLPSFEQCFPKSTKEHTEVVHEETGHVLKVPF
Sbjct: 61  LTFDPPETSTEKAKDRKHTIDPSSPDFLPLPSFEQCFPKSTKEHTEVVHEETGHVLKVPF 120

Query: 121 RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGSPRYTQMYYAKQGIIT 180
           RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGSPRYTQMYYAKQGIIT
Sbjct: 121 RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGSPRYTQMYYAKQGIIT 180

Query: 181 EEMLYCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA 240
           EEMLYCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA
Sbjct: 181 EEMLYCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA 240

Query: 241 SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI 300
           SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI
Sbjct: 241 SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI 300

Query: 301 AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH 360
           AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH
Sbjct: 301 AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH 360

Query: 361 HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 420
           HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK
Sbjct: 361 HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 420

Query: 421 DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG 480
           DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG
Sbjct: 421 DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG 480

Query: 481 ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE 540
           ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE
Sbjct: 481 ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE 540

Query: 541 FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY 600
           FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY
Sbjct: 541 FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY 600

Query: 601 GSAEEALQEGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 650
           GSAEEALQEGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR
Sbjct: 601 GSAEEALQEGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 649

BLAST of CSPI01G23750 vs. ExPASy TrEMBL
Match: A0A5A7SPA0 (Phosphomethylpyrimidine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold848G00490 PE=3 SV=1)

HSP 1 Score: 1310.8 bits (3391), Expect = 0.0e+00
Identity = 637/649 (98.15%), Postives = 643/649 (99.08%), Query Frame = 0

Query: 1   MASVHATITPAAAVGKSGNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRAT 60
           MASVHATITP  AVGKS NRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRAT
Sbjct: 1   MASVHATITP--AVGKSVNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRAT 60

Query: 61  LTFDPPETSTEKAKDRKHTIDPSSPDFLPLPSFEQCFPKSTKEHTEVVHEETGHVLKVPF 120
           LTFDPPETSTEKAK+ KHTIDPSSPDFLPLPSFEQCFPKSTKEH EVVHEETGHVLKVPF
Sbjct: 61  LTFDPPETSTEKAKNSKHTIDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPF 120

Query: 121 RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGSPRYTQMYYAKQGIIT 180
           RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLG+PR TQMYYAKQGIIT
Sbjct: 121 RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGAPRCTQMYYAKQGIIT 180

Query: 181 EEMLYCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA 240
           EEML+CATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA
Sbjct: 181 EEMLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA 240

Query: 241 SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI 300
           SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI
Sbjct: 241 SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI 300

Query: 301 AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH 360
           AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH
Sbjct: 301 AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH 360

Query: 361 HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 420
           HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK
Sbjct: 361 HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 420

Query: 421 DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG 480
           DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG
Sbjct: 421 DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG 480

Query: 481 ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE 540
           ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE
Sbjct: 481 ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE 540

Query: 541 FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY 600
           FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY
Sbjct: 541 FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY 600

Query: 601 GSAEEALQEGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 650
           G+AEEALQ+GMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYV+SQKR
Sbjct: 601 GTAEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVESQKR 647

BLAST of CSPI01G23750 vs. ExPASy TrEMBL
Match: A0A1S3C5I7 (phosphomethylpyrimidine synthase, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103497093 PE=3 SV=1)

HSP 1 Score: 1309.3 bits (3387), Expect = 0.0e+00
Identity = 636/649 (98.00%), Postives = 642/649 (98.92%), Query Frame = 0

Query: 1   MASVHATITPAAAVGKSGNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRAT 60
           MASVHATITP  AVGKS NRSSPTKSLNTAFLPGFDVVGRVAS CKDLHPSSITLAPRAT
Sbjct: 1   MASVHATITP--AVGKSVNRSSPTKSLNTAFLPGFDVVGRVASTCKDLHPSSITLAPRAT 60

Query: 61  LTFDPPETSTEKAKDRKHTIDPSSPDFLPLPSFEQCFPKSTKEHTEVVHEETGHVLKVPF 120
           LTFDPPETSTEKAK+ KHTIDPSSPDFLPLPSFEQCFPKSTKEH EVVHEETGHVLKVPF
Sbjct: 61  LTFDPPETSTEKAKNSKHTIDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPF 120

Query: 121 RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGSPRYTQMYYAKQGIIT 180
           RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLG+PR TQMYYAKQGIIT
Sbjct: 121 RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGAPRCTQMYYAKQGIIT 180

Query: 181 EEMLYCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA 240
           EEML+CATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA
Sbjct: 181 EEMLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA 240

Query: 241 SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI 300
           SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI
Sbjct: 241 SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI 300

Query: 301 AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH 360
           AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH
Sbjct: 301 AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH 360

Query: 361 HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 420
           HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK
Sbjct: 361 HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 420

Query: 421 DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG 480
           DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG
Sbjct: 421 DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG 480

Query: 481 ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE 540
           ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE
Sbjct: 481 ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE 540

Query: 541 FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY 600
           FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY
Sbjct: 541 FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY 600

Query: 601 GSAEEALQEGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 650
           G+AEEALQ+GMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYV+SQKR
Sbjct: 601 GTAEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVESQKR 647

BLAST of CSPI01G23750 vs. ExPASy TrEMBL
Match: A0A5D3BDX2 (Phosphomethylpyrimidine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001330 PE=3 SV=1)

HSP 1 Score: 1309.3 bits (3387), Expect = 0.0e+00
Identity = 636/649 (98.00%), Postives = 642/649 (98.92%), Query Frame = 0

Query: 1   MASVHATITPAAAVGKSGNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRAT 60
           MASVHATITP  AVGKS NRSSPTKSLNTAFLPGFDVVGRVAS CKDLHPSSITLAPRAT
Sbjct: 1   MASVHATITP--AVGKSVNRSSPTKSLNTAFLPGFDVVGRVASTCKDLHPSSITLAPRAT 60

Query: 61  LTFDPPETSTEKAKDRKHTIDPSSPDFLPLPSFEQCFPKSTKEHTEVVHEETGHVLKVPF 120
           LTFDPPETSTEKAK+ KHTIDPSSPDFLPLPSFEQCFPKSTKEH EVVHEETGHVLKVPF
Sbjct: 61  LTFDPPETSTEKAKNSKHTIDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPF 120

Query: 121 RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGSPRYTQMYYAKQGIIT 180
           RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLG+PR TQMYYAKQGIIT
Sbjct: 121 RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGAPRCTQMYYAKQGIIT 180

Query: 181 EEMLYCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA 240
           EEML+CATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA
Sbjct: 181 EEMLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA 240

Query: 241 SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI 300
           SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI
Sbjct: 241 SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI 300

Query: 301 AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH 360
           AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH
Sbjct: 301 AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH 360

Query: 361 HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 420
           HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK
Sbjct: 361 HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 420

Query: 421 DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG 480
           DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG
Sbjct: 421 DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG 480

Query: 481 ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE 540
           ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE
Sbjct: 481 ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE 540

Query: 541 FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY 600
           FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY
Sbjct: 541 FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY 600

Query: 601 GSAEEALQEGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 650
           G+AEEALQ+GMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYV+SQKR
Sbjct: 601 GTAEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVESQKR 647

BLAST of CSPI01G23750 vs. ExPASy TrEMBL
Match: A0A1S3C5D6 (phosphomethylpyrimidine synthase, chloroplastic isoform X3 OS=Cucumis melo OX=3656 GN=LOC103497093 PE=3 SV=1)

HSP 1 Score: 1309.3 bits (3387), Expect = 0.0e+00
Identity = 636/649 (98.00%), Postives = 642/649 (98.92%), Query Frame = 0

Query: 1    MASVHATITPAAAVGKSGNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRAT 60
            MASVHATITP  AVGKS NRSSPTKSLNTAFLPGFDVVGRVAS CKDLHPSSITLAPRAT
Sbjct: 421  MASVHATITP--AVGKSVNRSSPTKSLNTAFLPGFDVVGRVASTCKDLHPSSITLAPRAT 480

Query: 61   LTFDPPETSTEKAKDRKHTIDPSSPDFLPLPSFEQCFPKSTKEHTEVVHEETGHVLKVPF 120
            LTFDPPETSTEKAK+ KHTIDPSSPDFLPLPSFEQCFPKSTKEH EVVHEETGHVLKVPF
Sbjct: 481  LTFDPPETSTEKAKNSKHTIDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPF 540

Query: 121  RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGSPRYTQMYYAKQGIIT 180
            RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLG+PR TQMYYAKQGIIT
Sbjct: 541  RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGAPRCTQMYYAKQGIIT 600

Query: 181  EEMLYCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA 240
            EEML+CATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA
Sbjct: 601  EEMLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA 660

Query: 241  SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI 300
            SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI
Sbjct: 661  SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI 720

Query: 301  AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH 360
            AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH
Sbjct: 721  AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH 780

Query: 361  HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 420
            HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK
Sbjct: 781  HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 840

Query: 421  DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG 480
            DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG
Sbjct: 841  DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG 900

Query: 481  ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE 540
            ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE
Sbjct: 901  ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE 960

Query: 541  FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY 600
            FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY
Sbjct: 961  FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY 1020

Query: 601  GSAEEALQEGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 650
            G+AEEALQ+GMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYV+SQKR
Sbjct: 1021 GTAEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVESQKR 1067

BLAST of CSPI01G23750 vs. NCBI nr
Match: XP_011658531.1 (phosphomethylpyrimidine synthase, chloroplastic isoform X3 [Cucumis sativus])

HSP 1 Score: 1338.6 bits (3463), Expect = 0.0e+00
Identity = 649/649 (100.00%), Postives = 649/649 (100.00%), Query Frame = 0

Query: 1    MASVHATITPAAAVGKSGNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRAT 60
            MASVHATITPAAAVGKSGNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRAT
Sbjct: 591  MASVHATITPAAAVGKSGNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRAT 650

Query: 61   LTFDPPETSTEKAKDRKHTIDPSSPDFLPLPSFEQCFPKSTKEHTEVVHEETGHVLKVPF 120
            LTFDPPETSTEKAKDRKHTIDPSSPDFLPLPSFEQCFPKSTKEHTEVVHEETGHVLKVPF
Sbjct: 651  LTFDPPETSTEKAKDRKHTIDPSSPDFLPLPSFEQCFPKSTKEHTEVVHEETGHVLKVPF 710

Query: 121  RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGSPRYTQMYYAKQGIIT 180
            RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGSPRYTQMYYAKQGIIT
Sbjct: 711  RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGSPRYTQMYYAKQGIIT 770

Query: 181  EEMLYCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA 240
            EEMLYCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA
Sbjct: 771  EEMLYCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA 830

Query: 241  SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI 300
            SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI
Sbjct: 831  SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI 890

Query: 301  AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH 360
            AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH
Sbjct: 891  AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH 950

Query: 361  HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 420
            HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK
Sbjct: 951  HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 1010

Query: 421  DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG 480
            DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG
Sbjct: 1011 DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG 1070

Query: 481  ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE 540
            ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE
Sbjct: 1071 ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE 1130

Query: 541  FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY 600
            FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY
Sbjct: 1131 FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY 1190

Query: 601  GSAEEALQEGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 650
            GSAEEALQEGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR
Sbjct: 1191 GSAEEALQEGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 1239

BLAST of CSPI01G23750 vs. NCBI nr
Match: XP_004150622.1 (phosphomethylpyrimidine synthase, chloroplastic isoform X2 [Cucumis sativus] >XP_031745106.1 phosphomethylpyrimidine synthase, chloroplastic isoform X2 [Cucumis sativus] >KGN65794.1 hypothetical protein Csa_023161 [Cucumis sativus])

HSP 1 Score: 1338.6 bits (3463), Expect = 0.0e+00
Identity = 649/649 (100.00%), Postives = 649/649 (100.00%), Query Frame = 0

Query: 1   MASVHATITPAAAVGKSGNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRAT 60
           MASVHATITPAAAVGKSGNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRAT
Sbjct: 1   MASVHATITPAAAVGKSGNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRAT 60

Query: 61  LTFDPPETSTEKAKDRKHTIDPSSPDFLPLPSFEQCFPKSTKEHTEVVHEETGHVLKVPF 120
           LTFDPPETSTEKAKDRKHTIDPSSPDFLPLPSFEQCFPKSTKEHTEVVHEETGHVLKVPF
Sbjct: 61  LTFDPPETSTEKAKDRKHTIDPSSPDFLPLPSFEQCFPKSTKEHTEVVHEETGHVLKVPF 120

Query: 121 RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGSPRYTQMYYAKQGIIT 180
           RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGSPRYTQMYYAKQGIIT
Sbjct: 121 RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGSPRYTQMYYAKQGIIT 180

Query: 181 EEMLYCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA 240
           EEMLYCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA
Sbjct: 181 EEMLYCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA 240

Query: 241 SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI 300
           SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI
Sbjct: 241 SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI 300

Query: 301 AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH 360
           AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH
Sbjct: 301 AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH 360

Query: 361 HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 420
           HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK
Sbjct: 361 HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 420

Query: 421 DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG 480
           DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG
Sbjct: 421 DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG 480

Query: 481 ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE 540
           ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE
Sbjct: 481 ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE 540

Query: 541 FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY 600
           FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY
Sbjct: 541 FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY 600

Query: 601 GSAEEALQEGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 650
           GSAEEALQEGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR
Sbjct: 601 GSAEEALQEGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 649

BLAST of CSPI01G23750 vs. NCBI nr
Match: KAA0031806.1 (phosphomethylpyrimidine synthase [Cucumis melo var. makuwa])

HSP 1 Score: 1310.8 bits (3391), Expect = 0.0e+00
Identity = 637/649 (98.15%), Postives = 643/649 (99.08%), Query Frame = 0

Query: 1   MASVHATITPAAAVGKSGNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRAT 60
           MASVHATITP  AVGKS NRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRAT
Sbjct: 1   MASVHATITP--AVGKSVNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRAT 60

Query: 61  LTFDPPETSTEKAKDRKHTIDPSSPDFLPLPSFEQCFPKSTKEHTEVVHEETGHVLKVPF 120
           LTFDPPETSTEKAK+ KHTIDPSSPDFLPLPSFEQCFPKSTKEH EVVHEETGHVLKVPF
Sbjct: 61  LTFDPPETSTEKAKNSKHTIDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPF 120

Query: 121 RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGSPRYTQMYYAKQGIIT 180
           RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLG+PR TQMYYAKQGIIT
Sbjct: 121 RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGAPRCTQMYYAKQGIIT 180

Query: 181 EEMLYCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA 240
           EEML+CATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA
Sbjct: 181 EEMLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA 240

Query: 241 SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI 300
           SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI
Sbjct: 241 SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI 300

Query: 301 AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH 360
           AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH
Sbjct: 301 AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH 360

Query: 361 HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 420
           HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK
Sbjct: 361 HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 420

Query: 421 DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG 480
           DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG
Sbjct: 421 DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG 480

Query: 481 ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE 540
           ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE
Sbjct: 481 ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE 540

Query: 541 FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY 600
           FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY
Sbjct: 541 FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY 600

Query: 601 GSAEEALQEGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 650
           G+AEEALQ+GMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYV+SQKR
Sbjct: 601 GTAEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVESQKR 647

BLAST of CSPI01G23750 vs. NCBI nr
Match: XP_008457389.1 (PREDICTED: phosphomethylpyrimidine synthase, chloroplastic isoform X3 [Cucumis melo])

HSP 1 Score: 1309.3 bits (3387), Expect = 0.0e+00
Identity = 636/649 (98.00%), Postives = 642/649 (98.92%), Query Frame = 0

Query: 1    MASVHATITPAAAVGKSGNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRAT 60
            MASVHATITP  AVGKS NRSSPTKSLNTAFLPGFDVVGRVAS CKDLHPSSITLAPRAT
Sbjct: 421  MASVHATITP--AVGKSVNRSSPTKSLNTAFLPGFDVVGRVASTCKDLHPSSITLAPRAT 480

Query: 61   LTFDPPETSTEKAKDRKHTIDPSSPDFLPLPSFEQCFPKSTKEHTEVVHEETGHVLKVPF 120
            LTFDPPETSTEKAK+ KHTIDPSSPDFLPLPSFEQCFPKSTKEH EVVHEETGHVLKVPF
Sbjct: 481  LTFDPPETSTEKAKNSKHTIDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPF 540

Query: 121  RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGSPRYTQMYYAKQGIIT 180
            RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLG+PR TQMYYAKQGIIT
Sbjct: 541  RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGAPRCTQMYYAKQGIIT 600

Query: 181  EEMLYCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA 240
            EEML+CATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA
Sbjct: 601  EEMLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA 660

Query: 241  SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI 300
            SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI
Sbjct: 661  SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI 720

Query: 301  AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH 360
            AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH
Sbjct: 721  AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH 780

Query: 361  HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 420
            HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK
Sbjct: 781  HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 840

Query: 421  DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG 480
            DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG
Sbjct: 841  DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG 900

Query: 481  ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE 540
            ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE
Sbjct: 901  ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE 960

Query: 541  FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY 600
            FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY
Sbjct: 961  FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY 1020

Query: 601  GSAEEALQEGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 650
            G+AEEALQ+GMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYV+SQKR
Sbjct: 1021 GTAEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVESQKR 1067

BLAST of CSPI01G23750 vs. NCBI nr
Match: XP_008457386.1 (PREDICTED: phosphomethylpyrimidine synthase, chloroplastic isoform X2 [Cucumis melo] >XP_008457388.1 PREDICTED: phosphomethylpyrimidine synthase, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 1309.3 bits (3387), Expect = 0.0e+00
Identity = 636/649 (98.00%), Postives = 642/649 (98.92%), Query Frame = 0

Query: 1   MASVHATITPAAAVGKSGNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRAT 60
           MASVHATITP  AVGKS NRSSPTKSLNTAFLPGFDVVGRVAS CKDLHPSSITLAPRAT
Sbjct: 1   MASVHATITP--AVGKSVNRSSPTKSLNTAFLPGFDVVGRVASTCKDLHPSSITLAPRAT 60

Query: 61  LTFDPPETSTEKAKDRKHTIDPSSPDFLPLPSFEQCFPKSTKEHTEVVHEETGHVLKVPF 120
           LTFDPPETSTEKAK+ KHTIDPSSPDFLPLPSFEQCFPKSTKEH EVVHEETGHVLKVPF
Sbjct: 61  LTFDPPETSTEKAKNSKHTIDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPF 120

Query: 121 RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGSPRYTQMYYAKQGIIT 180
           RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLG+PR TQMYYAKQGIIT
Sbjct: 121 RRVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGAPRCTQMYYAKQGIIT 180

Query: 181 EEMLYCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA 240
           EEML+CATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA
Sbjct: 181 EEMLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVA 240

Query: 241 SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI 300
           SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI
Sbjct: 241 SSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGI 300

Query: 301 AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH 360
           AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH
Sbjct: 301 AENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAH 360

Query: 361 HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 420
           HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK
Sbjct: 361 HKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEK 420

Query: 421 DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG 480
           DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG
Sbjct: 421 DVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIG 480

Query: 481 ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE 540
           ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE
Sbjct: 481 ALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFE 540

Query: 541 FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY 600
           FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY
Sbjct: 541 FRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGY 600

Query: 601 GSAEEALQEGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 650
           G+AEEALQ+GMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYV+SQKR
Sbjct: 601 GTAEEALQQGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVESQKR 647

BLAST of CSPI01G23750 vs. TAIR 10
Match: AT2G29630.2 (thiaminC )

HSP 1 Score: 1163.7 bits (3009), Expect = 0.0e+00
Identity = 559/648 (86.27%), Postives = 599/648 (92.44%), Query Frame = 0

Query: 2   ASVHATITPAAAVGKSGNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRATL 61
           ASVH T+   + V  + N S+  K  N++ LPGFDVV + A+          T   RATL
Sbjct: 3   ASVHCTL--MSVVCNNKNHSARPKLPNSSLLPGFDVVVQAAAT----RFKKETTTTRATL 62

Query: 62  TFDPPETSTEKAKDRKHTIDPSSPDFLPLPSFEQCFPKSTKEHTEVVHEETGHVLKVPFR 121
           TFDPP T++E+AK RKHTIDPSSPDF P+PSFE+CFPKSTKEH EVVHEE+GHVLKVPFR
Sbjct: 63  TFDPPTTNSERAKQRKHTIDPSSPDFQPIPSFEECFPKSTKEHKEVVHEESGHVLKVPFR 122

Query: 122 RVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGSPRYTQMYYAKQGIITE 181
           RVHLSG EP FDNYDTSGPQN+N  IGL KLRK+W+DRR+KLG+PRYTQMYYAKQGIITE
Sbjct: 123 RVHLSGGEPAFDNYDTSGPQNVNAHIGLAKLRKEWIDRREKLGTPRYTQMYYAKQGIITE 182

Query: 182 EMLYCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVAS 241
           EMLYCATREKLDPEFVRSEVARGRAIIPSNK HLELEPMIVGR FLVKVNANIGNSAVAS
Sbjct: 183 EMLYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVAS 242

Query: 242 SIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGIA 301
           SIEEEVYKVQWATMWGADT+MDLSTGRHIHETREWILRNS+VPVGTVPIYQALEKV+GIA
Sbjct: 243 SIEEEVYKVQWATMWGADTIMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVDGIA 302

Query: 302 ENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHH 361
           ENL WE+FRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKR+TGIVSRGGSIHAKWCLA+H
Sbjct: 303 ENLNWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRLTGIVSRGGSIHAKWCLAYH 362

Query: 362 KENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKD 421
           KENFAYEHWDDILDICNQYD++LSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKD
Sbjct: 363 KENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKD 422

Query: 422 VQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGA 481
           VQVMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTD+APGYDHITSAIGAANIGA
Sbjct: 423 VQVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDIAPGYDHITSAIGAANIGA 482

Query: 482 LGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEF 541
           LGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK HPHAQ+WDDALSKARFEF
Sbjct: 483 LGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKQHPHAQAWDDALSKARFEF 542

Query: 542 RWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYG 601
           RWMDQFALSLDPMTAMSFHDETLP++GAKVAHFCSMCGPKFCSMKITED+RKYAEE+GYG
Sbjct: 543 RWMDQFALSLDPMTAMSFHDETLPADGAKVAHFCSMCGPKFCSMKITEDIRKYAEENGYG 602

Query: 602 SAEEALQEGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 650
           SAEEA+++GMDAMS EF  AKKTISGEQHGE GGEIYLP SYV + ++
Sbjct: 603 SAEEAIRQGMDAMSEEFNIAKKTISGEQHGEVGGEIYLPESYVKAAQK 644

BLAST of CSPI01G23750 vs. TAIR 10
Match: AT2G29630.1 (thiaminC )

HSP 1 Score: 1163.7 bits (3009), Expect = 0.0e+00
Identity = 559/648 (86.27%), Postives = 599/648 (92.44%), Query Frame = 0

Query: 2   ASVHATITPAAAVGKSGNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRATL 61
           ASVH T+   + V  + N S+  K  N++ LPGFDVV + A+          T   RATL
Sbjct: 3   ASVHCTL--MSVVCNNKNHSARPKLPNSSLLPGFDVVVQAAAT----RFKKETTTTRATL 62

Query: 62  TFDPPETSTEKAKDRKHTIDPSSPDFLPLPSFEQCFPKSTKEHTEVVHEETGHVLKVPFR 121
           TFDPP T++E+AK RKHTIDPSSPDF P+PSFE+CFPKSTKEH EVVHEE+GHVLKVPFR
Sbjct: 63  TFDPPTTNSERAKQRKHTIDPSSPDFQPIPSFEECFPKSTKEHKEVVHEESGHVLKVPFR 122

Query: 122 RVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGSPRYTQMYYAKQGIITE 181
           RVHLSG EP FDNYDTSGPQN+N  IGL KLRK+W+DRR+KLG+PRYTQMYYAKQGIITE
Sbjct: 123 RVHLSGGEPAFDNYDTSGPQNVNAHIGLAKLRKEWIDRREKLGTPRYTQMYYAKQGIITE 182

Query: 182 EMLYCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVAS 241
           EMLYCATREKLDPEFVRSEVARGRAIIPSNK HLELEPMIVGR FLVKVNANIGNSAVAS
Sbjct: 183 EMLYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVAS 242

Query: 242 SIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGIA 301
           SIEEEVYKVQWATMWGADT+MDLSTGRHIHETREWILRNS+VPVGTVPIYQALEKV+GIA
Sbjct: 243 SIEEEVYKVQWATMWGADTIMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVDGIA 302

Query: 302 ENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHH 361
           ENL WE+FRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKR+TGIVSRGGSIHAKWCLA+H
Sbjct: 303 ENLNWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRLTGIVSRGGSIHAKWCLAYH 362

Query: 362 KENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKD 421
           KENFAYEHWDDILDICNQYD++LSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKD
Sbjct: 363 KENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKD 422

Query: 422 VQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGA 481
           VQVMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTD+APGYDHITSAIGAANIGA
Sbjct: 423 VQVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDIAPGYDHITSAIGAANIGA 482

Query: 482 LGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEF 541
           LGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK HPHAQ+WDDALSKARFEF
Sbjct: 483 LGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKQHPHAQAWDDALSKARFEF 542

Query: 542 RWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYG 601
           RWMDQFALSLDPMTAMSFHDETLP++GAKVAHFCSMCGPKFCSMKITED+RKYAEE+GYG
Sbjct: 543 RWMDQFALSLDPMTAMSFHDETLPADGAKVAHFCSMCGPKFCSMKITEDIRKYAEENGYG 602

Query: 602 SAEEALQEGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 650
           SAEEA+++GMDAMS EF  AKKTISGEQHGE GGEIYLP SYV + ++
Sbjct: 603 SAEEAIRQGMDAMSEEFNIAKKTISGEQHGEVGGEIYLPESYVKAAQK 644

BLAST of CSPI01G23750 vs. TAIR 10
Match: AT2G29630.3 (thiaminC )

HSP 1 Score: 1163.7 bits (3009), Expect = 0.0e+00
Identity = 559/648 (86.27%), Postives = 599/648 (92.44%), Query Frame = 0

Query: 2   ASVHATITPAAAVGKSGNRSSPTKSLNTAFLPGFDVVGRVASACKDLHPSSITLAPRATL 61
           ASVH T+   + V  + N S+  K  N++ LPGFDVV + A+          T   RATL
Sbjct: 3   ASVHCTL--MSVVCNNKNHSARPKLPNSSLLPGFDVVVQAAAT----RFKKETTTTRATL 62

Query: 62  TFDPPETSTEKAKDRKHTIDPSSPDFLPLPSFEQCFPKSTKEHTEVVHEETGHVLKVPFR 121
           TFDPP T++E+AK RKHTIDPSSPDF P+PSFE+CFPKSTKEH EVVHEE+GHVLKVPFR
Sbjct: 63  TFDPPTTNSERAKQRKHTIDPSSPDFQPIPSFEECFPKSTKEHKEVVHEESGHVLKVPFR 122

Query: 122 RVHLSGDEPNFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGSPRYTQMYYAKQGIITE 181
           RVHLSG EP FDNYDTSGPQN+N  IGL KLRK+W+DRR+KLG+PRYTQMYYAKQGIITE
Sbjct: 123 RVHLSGGEPAFDNYDTSGPQNVNAHIGLAKLRKEWIDRREKLGTPRYTQMYYAKQGIITE 182

Query: 182 EMLYCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVAS 241
           EMLYCATREKLDPEFVRSEVARGRAIIPSNK HLELEPMIVGR FLVKVNANIGNSAVAS
Sbjct: 183 EMLYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVAS 242

Query: 242 SIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGIA 301
           SIEEEVYKVQWATMWGADT+MDLSTGRHIHETREWILRNS+VPVGTVPIYQALEKV+GIA
Sbjct: 243 SIEEEVYKVQWATMWGADTIMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVDGIA 302

Query: 302 ENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHH 361
           ENL WE+FRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKR+TGIVSRGGSIHAKWCLA+H
Sbjct: 303 ENLNWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRLTGIVSRGGSIHAKWCLAYH 362

Query: 362 KENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKD 421
           KENFAYEHWDDILDICNQYD++LSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKD
Sbjct: 363 KENFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKD 422

Query: 422 VQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGA 481
           VQVMNEGPGH+PMHKIPENMQKQLEWCNEAPFYTLGPLTTD+APGYDHITSAIGAANIGA
Sbjct: 423 VQVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDIAPGYDHITSAIGAANIGA 482

Query: 482 LGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEF 541
           LGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAK HPHAQ+WDDALSKARFEF
Sbjct: 483 LGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKQHPHAQAWDDALSKARFEF 542

Query: 542 RWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYG 601
           RWMDQFALSLDPMTAMSFHDETLP++GAKVAHFCSMCGPKFCSMKITED+RKYAEE+GYG
Sbjct: 543 RWMDQFALSLDPMTAMSFHDETLPADGAKVAHFCSMCGPKFCSMKITEDIRKYAEENGYG 602

Query: 602 SAEEALQEGMDAMSAEFLAAKKTISGEQHGETGGEIYLPASYVDSQKR 650
           SAEEA+++GMDAMS EF  AKKTISGEQHGE GGEIYLP SYV + ++
Sbjct: 603 SAEEAIRQGMDAMSEEFNIAKKTISGEQHGEVGGEIYLPESYVKAAQK 644

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O823920.0e+0086.27Phosphomethylpyrimidine synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
Q9KBJ41.1e-20963.64Phosphomethylpyrimidine synthase OS=Bacillus halodurans (strain ATCC BAA-125 / D... [more]
A7GUZ51.5e-20664.12Phosphomethylpyrimidine synthase OS=Bacillus cytotoxicus (strain DSM 22905 / CIP... [more]
A4VR381.5e-20661.50Phosphomethylpyrimidine synthase OS=Pseudomonas stutzeri (strain A1501) OX=37973... [more]
A6VD864.3e-20661.33Phosphomethylpyrimidine synthase OS=Pseudomonas aeruginosa (strain PA7) OX=38175... [more]
Match NameE-valueIdentityDescription
A0A0A0LXK60.0e+00100.00Catalytic OS=Cucumis sativus OX=3659 GN=Csa_1G528590 PE=3 SV=1[more]
A0A5A7SPA00.0e+0098.15Phosphomethylpyrimidine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A1S3C5I70.0e+0098.00phosphomethylpyrimidine synthase, chloroplastic isoform X2 OS=Cucumis melo OX=36... [more]
A0A5D3BDX20.0e+0098.00Phosphomethylpyrimidine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3C5D60.0e+0098.00phosphomethylpyrimidine synthase, chloroplastic isoform X3 OS=Cucumis melo OX=36... [more]
Match NameE-valueIdentityDescription
XP_011658531.10.0e+00100.00phosphomethylpyrimidine synthase, chloroplastic isoform X3 [Cucumis sativus][more]
XP_004150622.10.0e+00100.00phosphomethylpyrimidine synthase, chloroplastic isoform X2 [Cucumis sativus] >XP... [more]
KAA0031806.10.0e+0098.15phosphomethylpyrimidine synthase [Cucumis melo var. makuwa][more]
XP_008457389.10.0e+0098.00PREDICTED: phosphomethylpyrimidine synthase, chloroplastic isoform X3 [Cucumis m... [more]
XP_008457386.10.0e+0098.00PREDICTED: phosphomethylpyrimidine synthase, chloroplastic isoform X2 [Cucumis m... [more]
Match NameE-valueIdentityDescription
AT2G29630.20.0e+0086.27thiaminC [more]
AT2G29630.10.0e+0086.27thiaminC [more]
AT2G29630.30.0e+0086.27thiaminC [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002817Phosphomethylpyrimidine synthase ThiC/5-hydroxybenzimidazole synthase BzaA/BTIGRFAMTIGR00190TIGR00190coord: 169..592
e-value: 1.6E-204
score: 677.4
IPR002817Phosphomethylpyrimidine synthase ThiC/5-hydroxybenzimidazole synthase BzaA/BPFAMPF01964ThiC_Rad_SAMcoord: 169..589
e-value: 6.2E-198
score: 657.7
IPR002817Phosphomethylpyrimidine synthase ThiC/5-hydroxybenzimidazole synthase BzaA/BSFLDSFLDF00407phosphomethylpyrimidine_synthcoord: 169..589
e-value: 0.0
score: 658.9
IPR002817Phosphomethylpyrimidine synthase ThiC/5-hydroxybenzimidazole synthase BzaA/BPANTHERPTHR30557THIAMINE BIOSYNTHESIS PROTEIN THICcoord: 1..649
NoneNo IPR availableGENE3D6.10.250.620coord: 529..561
e-value: 3.9E-18
score: 67.0
NoneNo IPR availableSFLDSFLDS00113Radical_SAM_Phosphomethylpyricoord: 167..625
e-value: 0.0
score: 858.5
NoneNo IPR availableSFLDSFLDG01114phosphomethylpyrimidine_synthcoord: 167..623
e-value: 0.0
score: 839.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 61..85
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 68..82
NoneNo IPR availablePANTHERPTHR30557:SF2SUBFAMILY NOT NAMEDcoord: 1..649
IPR038521ThiC/Bza, core domainGENE3D3.20.20.540Radical SAM ThiC family, central domaincoord: 211..525
e-value: 8.5E-169
score: 562.2
IPR037509Phosphomethylpyrimidine synthaseHAMAPMF_00089ThiCcoord: 168..594
score: 95.084618

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G23750.1CSPI01G23750.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009228 thiamine biosynthetic process
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0016830 carbon-carbon lyase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0051536 iron-sulfur cluster binding