CSPI01G23400 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G23400
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Metal tolerance protein B
Location	Chr1: 18986485 .. 18988412 (+)
RNA-Seq Expression	CSPI01G23400
Synteny	CSPI01G23400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCTTTATTAGGTAATGAATTGTTTTGCTATATAGTTCTGTAACTTTCCTTTTTTATGAATTCGTCTGTTTCCTTTTCTCAGTGGCTCGCGGTTATTTTGTACAAATTGATATCCTGGACCTCTTTCTTATTGTTGATTATACGATTGAATTGTGCATTCTATCGATTCCTTGTTCATTTGTTCGTTTTGGGGGCTGATCGACCCAGAGTAGCGATTGTTGCATATTTTCCTGGAGGGGCTTGGTTTTTGAATCTTTGAGCATTTTTTAATTGTTAGTTCGTGTTTGTTTTTTCTTCTTGAGATATTCTTATGTAGGTTAATGAAGCTTACTTTTGATTCTTCTTTTTGCAGGTTTTTTCGTATTGATTTATATATATTGGATAGGTCAAGGAAATGGGGGAGGAGGAAGTGCTTATTTTGAAAACAGAACACTCGGATGAAATTAACATCCCAATAGTTGCTAAGAAGATGAATGATGTTATTCCCACTTCCACTTCCTCAGAAGTTAAATGTTGCAGTTCTGGCTGTGCTTTTTCCAGATTAGAACATAGTAATTTGGAGTCATTAAAACGTTCTAAATCAGCTATGAAGCTTGGTGGATTGATATTGTTTTATACTATAGCTATAGTAGTTGAGATTATTGGTGGTTTGAGAGCTAACAGTCTTTCGGTAATGACAGATGCAGCTCACTTGCTTAGTGATGTTGCTGGTTTCTCTGTTTCCCTCTTTGCAGTTTGGGTTTCGGGTTGGGAGGCGACACCGCAACACTCGTTCGGGTATAACCGTCTTGAAGTTTTAGGTGCCCTTGTGTCTGTACAACTTATATGGCTGATTTCTGGGATATTAATCTACGAGGCGATTGACCGGATTCTTGCACCAAAGACTAAGGTGGATGGTTTCCTTATGTTTGCCGTTGCAGCTTTTGGATTCCTTCTGAACTTGTTTATGGTTATTTGGCTTGGCCATAGCCACCACCACCACCACTCTCATTCATCTCATTGTTGCCATCATGATCATCACTCTCATTCTCATCAGAATCACTTGGAGCATGAGGAGGAGGAGGTTTACACATTAACCAAACAAGAAGGTGCTTCTTTGGGATCAAAGGACAACAGTTCAACGTTGAACATAAATCTCCAAGGCGCTTATCTTCATGTCATTACTGATATGATTCAATCTATTGGTGTGATGATTGCTGGATTGGTTCTTTGGTTTAAACCCGAGTGGATCGTCGTAGATTTAATCTGCACTCTTGTATTTTCTGTTCTTGCTTTAGCTACTACTTTCTCTATGCTTAGACATACAGCTGTTATATTAATGGAAGGAACACCTAGGGAGGTCCATATTGAGAGCTTAGAAAATGACATCAAGAACATAAAAGGAGTTTATGATTTGCATGACTTGCACATTTGGTCCATAACGGTTGGAAAAGTCGTGTTGTCATGCCACGTTGTTGCTGAAGCCGGAGTTTGTTCTAGGGAGCTAATTTTGAAGATTAAAAGCCATTGTGAAAAGAGATACAATATAGTCCATACCACCATACAAGTTGAGTAAGGAGAAATGGGGAAACAAGAGCAAAAGAAAGGGAATGTAACAAAGTGTACTTGTTGCCTTGGTATAATTGAGTTATCATTCAGGTGACAGAACATCTACTCTTCTTCCAATTCAAGAGTTCAACAATATGCCATGTGAGGAGCTTGAACTTTTTATCTTTTGGCCGGGAGTAGATTATTCGTGTTCGTAACATCTTGTTATTTCTTGAGCATTCGAAAAGCTGACAGTTTGTACCAAGAGTTTTATACACAATGGACATTCATTTTATTTGTTCATTGTTCTTGAAGTTCCAATCACCAATAATTGTTCTATCAATGAATTCTCTGATCTCACATCCCCACAAATTTCTCAAGTTTGATAAAATTTCACAATGG

mRNA sequence

CCTTTATTAGGTAATGAATTGTTTTGCTATATAGTTCTGTAACTTTCCTTTTTTATGAATTCGTCTGTTTCCTTTTCTCAGTGGCTCGCGGTTATTTTGTACAAATTGATATCCTGGACCTCTTTCTTATTGTTGATTATACGATTGAATTGTGCATTCTATCGATTCCTTGTTCATTTGTTCGTTTTGGGGGCTGATCGACCCAGAGTAGCGATTGTTGCATATTTTCCTGGAGGGGCTTGGTTTTTGAATCTTTGAGCATTTTTTAATTGTTAGTTCGTGTTTGTTTTTTCTTCTTGAGATATTCTTATGTAGGTTAATGAAGCTTACTTTTGATTCTTCTTTTTGCAGGTTTTTTCGTATTGATTTATATATATTGGATAGGTCAAGGAAATGGGGGAGGAGGAAGTGCTTATTTTGAAAACAGAACACTCGGATGAAATTAACATCCCAATAGTTGCTAAGAAGATGAATGATGTTATTCCCACTTCCACTTCCTCAGAAGTTAAATGTTGCAGTTCTGGCTGTGCTTTTTCCAGATTAGAACATAGTAATTTGGAGTCATTAAAACGTTCTAAATCAGCTATGAAGCTTGGTGGATTGATATTGTTTTATACTATAGCTATAGTAGTTGAGATTATTGGTGGTTTGAGAGCTAACAGTCTTTCGGTAATGACAGATGCAGCTCACTTGCTTAGTGATGTTGCTGGTTTCTCTGTTTCCCTCTTTGCAGTTTGGGTTTCGGGTTGGGAGGCGACACCGCAACACTCGTTCGGGTATAACCGTCTTGAAGTTTTAGGTGCCCTTGTGTCTGTACAACTTATATGGCTGATTTCTGGGATATTAATCTACGAGGCGATTGACCGGATTCTTGCACCAAAGACTAAGGTGGATGGTTTCCTTATGTTTGCCGTTGCAGCTTTTGGATTCCTTCTGAACTTGTTTATGGTTATTTGGCTTGGCCATAGCCACCACCACCACCACTCTCATTCATCTCATTGTTGCCATCATGATCATCACTCTCATTCTCATCAGAATCACTTGGAGCATGAGGAGGAGGAGGTTTACACATTAACCAAACAAGAAGGTGCTTCTTTGGGATCAAAGGACAACAGTTCAACGTTGAACATAAATCTCCAAGGCGCTTATCTTCATGTCATTACTGATATGATTCAATCTATTGGTGTGATGATTGCTGGATTGGTTCTTTGGTTTAAACCCGAGTGGATCGTCGTAGATTTAATCTGCACTCTTGTATTTTCTGTTCTTGCTTTAGCTACTACTTTCTCTATGCTTAGACATACAGCTGTTATATTAATGGAAGGAACACCTAGGGAGGTCCATATTGAGAGCTTAGAAAATGACATCAAGAACATAAAAGGAGTTTATGATTTGCATGACTTGCACATTTGGTCCATAACGGTTGGAAAAGTCGTGTTGTCATGCCACGTTGTTGCTGAAGCCGGAGTTTGTTCTAGGGAGCTAATTTTGAAGATTAAAAGCCATTGTGAAAAGAGATACAATATAGTCCATACCACCATACAAGTTGAGTAAGGAGAAATGGGGAAACAAGAGCAAAAGAAAGGGAATGTAACAAAGTGTACTTGTTGCCTTGGTATAATTGAGTTATCATTCAGGTGACAGAACATCTACTCTTCTTCCAATTCAAGAGTTCAACAATATGCCATGTGAGGAGCTTGAACTTTTTATCTTTTGGCCGGGAGTAGATTATTCGTGTTCGTAACATCTTGTTATTTCTTGAGCATTCGAAAAGCTGACAGTTTGTACCAAGAGTTTTATACACAATGGACATTCATTTTATTTGTTCATTGTTCTTGAAGTTCCAATCACCAATAATTGTTCTATCAATGAATTCTCTGATCTCACATCCCCACAAATTTCTCAAGTTTGATAAAATTTCACAATGG

Coding sequence (CDS)

ATGGGGGAGGAGGAAGTGCTTATTTTGAAAACAGAACACTCGGATGAAATTAACATCCCAATAGTTGCTAAGAAGATGAATGATGTTATTCCCACTTCCACTTCCTCAGAAGTTAAATGTTGCAGTTCTGGCTGTGCTTTTTCCAGATTAGAACATAGTAATTTGGAGTCATTAAAACGTTCTAAATCAGCTATGAAGCTTGGTGGATTGATATTGTTTTATACTATAGCTATAGTAGTTGAGATTATTGGTGGTTTGAGAGCTAACAGTCTTTCGGTAATGACAGATGCAGCTCACTTGCTTAGTGATGTTGCTGGTTTCTCTGTTTCCCTCTTTGCAGTTTGGGTTTCGGGTTGGGAGGCGACACCGCAACACTCGTTCGGGTATAACCGTCTTGAAGTTTTAGGTGCCCTTGTGTCTGTACAACTTATATGGCTGATTTCTGGGATATTAATCTACGAGGCGATTGACCGGATTCTTGCACCAAAGACTAAGGTGGATGGTTTCCTTATGTTTGCCGTTGCAGCTTTTGGATTCCTTCTGAACTTGTTTATGGTTATTTGGCTTGGCCATAGCCACCACCACCACCACTCTCATTCATCTCATTGTTGCCATCATGATCATCACTCTCATTCTCATCAGAATCACTTGGAGCATGAGGAGGAGGAGGTTTACACATTAACCAAACAAGAAGGTGCTTCTTTGGGATCAAAGGACAACAGTTCAACGTTGAACATAAATCTCCAAGGCGCTTATCTTCATGTCATTACTGATATGATTCAATCTATTGGTGTGATGATTGCTGGATTGGTTCTTTGGTTTAAACCCGAGTGGATCGTCGTAGATTTAATCTGCACTCTTGTATTTTCTGTTCTTGCTTTAGCTACTACTTTCTCTATGCTTAGACATACAGCTGTTATATTAATGGAAGGAACACCTAGGGAGGTCCATATTGAGAGCTTAGAAAATGACATCAAGAACATAAAAGGAGTTTATGATTTGCATGACTTGCACATTTGGTCCATAACGGTTGGAAAAGTCGTGTTGTCATGCCACGTTGTTGCTGAAGCCGGAGTTTGTTCTAGGGAGCTAATTTTGAAGATTAAAAGCCATTGTGAAAAGAGATACAATATAGTCCATACCACCATACAAGTTGAGTAA

Protein sequence

MGEEEVLILKTEHSDEINIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFLLNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHQNHLEHEEEEVYTLTKQEGASLGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALATTFSMLRHTAVILMEGTPREVHIESLENDIKNIKGVYDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCEKRYNIVHTTIQVE*

Homology

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BLAST of CSPI01G23400 vs. ExPASy Swiss-Prot
Match: Q6DBM8 (Metal tolerance protein B OS=Arabidopsis thaliana OX=3702 GN=MTPB PE=2 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 1.8e-98
Identity = 190/384 (49.48%), Postives = 260/384 (67.71%), Query Frame = 0


Query: 4   EEVLILKTEHSDEINIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKRSKS 63
           E++ ILK +  +E+  P          P+ T   +      CAF+R EH   E+ +R +S
Sbjct: 4   EQICILKPDDEEEMESP---------SPSKTEENLGVVPLSCAFTRQEHCVSETKEREES 63

Query: 64  AMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATP 123
             +L  LI  Y I + V+I+GG +ANSL+VMTDAAHLLSDVAG  VSL A+ VS WEA P
Sbjct: 64  TRRLSSLIFLYLIVMSVQIVGGFKANSLAVMTDAAHLLSDVAGLCVSLLAIKVSSWEANP 123

Query: 124 QHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFLLNL 183
           ++SFG+ RLEVL A +SVQLIWL+SG++I+EAI R+L+   +V+G +MF ++AFGF +NL
Sbjct: 124 RNSFGFKRLEVLAAFLSVQLIWLVSGVIIHEAIQRLLSRSREVNGEIMFGISAFGFFMNL 183

Query: 184 FMVIWLGHSH-HHHHSHSSHCCHHDHHSHSHQNHLEHEEEEVYTLTKQEGASLGSKDNSS 243
            MV+WLGH+H HHHH H     HH HH+H HQ+   H+E       ++     G K +S 
Sbjct: 184 VMVLWLGHNHSHHHHDH-----HHHHHNHKHQHQHHHKEVVAEEEEEEMNPLKGEKSSSK 243

Query: 244 TLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALATTFSMLR 303
            +NIN+QGAYLH + DMIQS+GVMI G ++W KP+W++VDLICTLVFS  ALA T  +L+
Sbjct: 244 EMNINIQGAYLHAMADMIQSLGVMIGGGIIWVKPKWVLVDLICTLVFSAFALAATLPILK 303

Query: 304 HTAVILMEGTPREVHIESLENDIKNIKGVYDLHDLHIWSITVGKVVLSCHVVAEAGVCSR 363
           +   ILME  PR++ IE LE  +K I GV  ++DLH+W ITVG++VLSCH++ E G   +
Sbjct: 304 NIFGILMERVPRDMDIEKLERGLKRIDGVKIVYDLHVWEITVGRIVLSCHILPEPGASPK 363

Query: 364 ELILKIKSHCEKRYNIVHTTIQVE 387
           E+I  +++ C K Y I H T+QVE
Sbjct: 364 EIITGVRNFCRKSYGIYHATVQVE 373

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BLAST of CSPI01G23400 vs. ExPASy Swiss-Prot
Match: Q9ZT63 (Metal tolerance protein 1 OS=Arabidopsis thaliana OX=3702 GN=MTP1 PE=1 SV=2)

HSP 1 Score: 312.8 bits (800), Expect = 5.6e-84
Identity = 168/367 (45.78%), Postives = 244/367 (66.49%), Query Frame = 0


Query: 40  CCSSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAH 99
           C  + C FS  ++++ ++ +RS S  KL   ++   + + VE++GG++ANSL+++TDAAH
Sbjct: 31  CGEAPCGFSDSKNASGDAHERSASMRKLCIAVVLCLVFMSVEVVGGIKANSLAILTDAAH 90

Query: 100 LLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRI 159
           LLSDVA F++SLF++W +GWEATP+ ++G+ R+E+LGALVS+QLIWL++GIL+YEAI RI
Sbjct: 91  LLSDVAAFAISLFSLWAAGWEATPRQTYGFFRIEILGALVSIQLIWLLTGILVYEAIIRI 150

Query: 160 LAPKTKVDGFLMFAVAAFGFLLNLFMVIWLGHSH-------------HHHHSH----SSH 219
           +   ++V+GFLMF VAAFG ++N+ M + LGH H             HH+HSH    ++H
Sbjct: 151 VTETSEVNGFLMFLVAAFGLVVNIIMAVLLGHDHGHSHGHGHGHGHDHHNHSHGVTVTTH 210

Query: 220 CCHHDH---HSHSHQNHLEHEEEEVYTLTKQEGASLGSKDNSSTLNINLQGAYLHVITDM 279
             HHDH   HSH H     H   +V      +  +  +       NINLQGAYLHV+ D 
Sbjct: 211 HHHHDHEHGHSHGHGEDKHHAHGDVTEQLLDKSKTQVAAKEKRKRNINLQGAYLHVLGDS 270

Query: 280 IQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALATTFSMLRHTAVILMEGTPREVHIE 339
           IQS+GVMI G ++W+ PEW +VDLICTL FSV+ L TT +M+R+   +LME TPRE+   
Sbjct: 271 IQSVGVMIGGAIIWYNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTPREIDAT 330

Query: 340 SLENDIKNIKGVYDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCEKRYNIV 387
            LE  +  ++ V  +H+LHIW+ITVGKV+L+CHV       +  ++ K+  +  + YNI 
Sbjct: 331 KLEKGLLEMEEVVAVHELHIWAITVGKVLLACHVNIRPEADADMVLNKVIDYIRREYNIS 390

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BLAST of CSPI01G23400 vs. ExPASy Swiss-Prot
Match: Q688R1 (Metal tolerance protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP1 PE=2 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 2.1e-83
Identity = 177/406 (43.60%), Postives = 252/406 (62.07%), Query Frame = 0


Query: 21  IVAKKMNDVIPTSTSSEVKCC-SSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIV 80
           I   +M+    TS ++  K C  + C FS   +S+ ++ +R  S  KL   ++   I + 
Sbjct: 11  IAEVRMDISSSTSVAAGNKVCRGAACDFSDSSNSSKDARERMASMRKLIIAVILCIIFMA 70

Query: 81  VEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALV 140
           VE++GG++ANSL+++TDAAHLLSDVA F++SLF++W +GWEATPQ S+G+ R+E+LGALV
Sbjct: 71  VEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPQQSYGFFRIEILGALV 130

Query: 141 SVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFLLNLFMVIWLGHSHHHHHSH 200
           S+QLIWL++GIL+YEAI R++    +V G LMFAV+AFG  +N+ M + LGH H H H H
Sbjct: 131 SIQLIWLLAGILVYEAIVRLINESGEVQGSLMFAVSAFGLFVNIIMAVLLGHDHGHGHGH 190

Query: 201 SSHCCH----------HDHHSHSHQNH---LEHEEEEVYTLTKQ---------------- 260
                H          HDHH H  Q H     HE+    ++T                  
Sbjct: 191 GHGHGHSHDHDHGGSDHDHHHHEDQEHGHVHHHEDGHGNSITVNLHHHPGTGHHHHDAEE 250

Query: 261 ---------EGASLGSKD-NSSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIV 320
                    +    G+KD   +  NIN+  AYLHV+ D IQSIGVMI G ++W+KPEW +
Sbjct: 251 PLLKSDAGCDSTQSGAKDAKKARRNINVHSAYLHVLGDSIQSIGVMIGGAIIWYKPEWKI 310

Query: 321 VDLICTLVFSVLALATTFSMLRHTAVILMEGTPREVHIESLENDIKNIKGVYDLHDLHIW 380
           +DLICTL+FSV+ L TT  MLR+   +LME TPRE+   SLEN ++++ GV  +H+LHIW
Sbjct: 311 IDLICTLIFSVIVLFTTIKMLRNILEVLMESTPREIDATSLENGLRDMDGVVAVHELHIW 370

Query: 381 SITVGKVVLSCHVVAEAGVCSRELILKIKSHCEKRYNIVHTTIQVE 387
           +ITVGKV+L+CHV       + +++ K+  + +  YNI H TIQ+E
Sbjct: 371 AITVGKVLLACHVTITQDADADQMLDKVIGYIKSEYNISHVTIQIE 416

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BLAST of CSPI01G23400 vs. ExPASy Swiss-Prot
Match: Q9LXS1 (Metal tolerance protein A2 OS=Arabidopsis thaliana OX=3702 GN=MTPA2 PE=2 SV=2)

HSP 1 Score: 306.2 bits (783), Expect = 5.2e-82
Identity = 167/348 (47.99%), Postives = 236/348 (67.82%), Query Frame = 0


Query: 40  CCSSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAH 99
           C  + C FS  + S++E+ +R+ S  KL   +L   I IVVE++GG++ANSL+++TDAAH
Sbjct: 47  CGEAPCGFSDAKTSSIEAQERAASMRKLLIAVLLCAIFIVVEVVGGIKANSLAILTDAAH 106

Query: 100 LLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRI 159
           LLSDVA F++SLF++W SGW+A PQ S+G+ R+E+LGALVS+Q+IWL++GIL+YEAI R+
Sbjct: 107 LLSDVAAFAISLFSLWASGWKANPQQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRL 166

Query: 160 LAPKTKVDGFLMFAVAAFGFLLNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHQ-NHLE 219
                +V+G LMFAV+A G L+N+ M I LGH H H H HS    H   H H H     E
Sbjct: 167 NNGSGEVEGSLMFAVSAVGLLVNIAMAILLGHDHGHGHGHSHDNGHGHSHDHGHGIAATE 226

Query: 220 HEEEEVYTLTKQEGASLGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEW 279
           H  +  +  ++     +  K      N+N+QGAYLHV+ D IQS+GVMI G ++W+KPEW
Sbjct: 227 HHHDSGHDESQLSDVLIEQKKQR---NVNIQGAYLHVLGDSIQSVGVMIGGAIIWYKPEW 286

Query: 280 IVVDLICTLVFSVLALATTFSMLRHTAVILMEGTPREVHIESLENDIKNIKGVYDLHDLH 339
            ++DLICTLVFSV+ L TT  MLR+   +LME TPRE+    LE  +  I+ V  +H+LH
Sbjct: 287 KILDLICTLVFSVIVLGTTIGMLRNILEVLMESTPREIDPTMLEKGVCEIEEVVAVHELH 346

Query: 340 IWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCEKRYNIVHTTIQVE 387
           IW+ITVGK++L+CHV       +  ++ KI  + ++ +NI H TIQ+E
Sbjct: 347 IWAITVGKLLLACHVKIRPEAEADMVLDKIIDYIKREHNISHVTIQIE 391

[Close]

BLAST of CSPI01G23400 vs. ExPASy Swiss-Prot
Match: Q9M271 (Metal tolerance protein A1 OS=Arabidopsis thaliana OX=3702 GN=MTPA1 PE=2 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 1.8e-74
Identity = 151/348 (43.39%), Postives = 220/348 (63.22%), Query Frame = 0


Query: 40  CCSSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAH 99
           C  + C FS    S+ ++ KR+ S  KL  +++   + + +E++ G++ANSL+++ DAAH
Sbjct: 9   CGETACGFST---SSSDAKKRAASMRKLCFVVVLCLLFMSIEVVCGIKANSLAILADAAH 68

Query: 100 LLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRI 159
           LL+DV  F++S+ ++W S WEA P+ S+G+ R+E+LG LVS+QLIWL++GIL+YEA+ R+
Sbjct: 69  LLTDVGAFAISMLSLWASSWEANPRQSYGFFRIEILGTLVSIQLIWLLTGILVYEAVTRL 128

Query: 160 L-APKTKVDGFLMFAVAAFGFLLNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHQNHLE 219
           +      VDGF M  VAAFG ++N+ M++ LGH H H H H         HSH H +   
Sbjct: 129 VQETNDDVDGFFMVLVAAFGLVVNIIMIVVLGHDHGHGHDHG--------HSHDHGHSYG 188

Query: 220 HEEEEVYTLTKQEGASLGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEW 279
              E++   +K+              NIN+QGAYLHV+ D+IQSIGVMI G ++W+ P+W
Sbjct: 189 ERAEQLLEKSKE------------IRNINVQGAYLHVLGDLIQSIGVMIGGGMIWYNPKW 248

Query: 280 IVVDLICTLVFSVLALATTFSMLRHTAVILMEGTPREVHIESLENDIKNIKGVYDLHDLH 339
            V+DLICTL FSV+ L TT  MLR    +LME TPRE+    LE  +  I+ V D+H+LH
Sbjct: 249 KVIDLICTLFFSVIVLGTTIKMLRSILEVLMESTPREIDARQLEKGLMEIEEVVDVHELH 308

Query: 340 IWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCEKRYNIVHTTIQVE 387
           IW+ITVGK + SCHV          ++ K+  +  + Y I H TIQ+E
Sbjct: 309 IWAITVGKALFSCHVKVRPEAGDEMVLNKVIDYIWREYRISHVTIQIE 333

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BLAST of CSPI01G23400 vs. ExPASy TrEMBL
Match: I1ZI47 (Metal transport protein 4 OS=Cucumis sativus OX=3659 GN=MTP4 PE=2 SV=1)

HSP 1 Score: 742.3 bits (1915), Expect = 1.0e-210
Identity = 385/386 (99.74%), Postives = 386/386 (100.00%), Query Frame = 0


Query: 1   MGEEEVLILKTEHSDEINIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKR 60
           MGEEEVLILKTEHSDEINIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKR
Sbjct: 1   MGEEEVLILKTEHSDEINIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKR 60

Query: 61  SKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120
           SKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE
Sbjct: 61  SKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120

Query: 121 ATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFL 180
           ATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFL
Sbjct: 121 ATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFL 180

Query: 181 LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHQNHLEHEEEEVYTLTKQEGASLGSKDN 240
           LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHQNHLEHEEEEVYTLTKQEGASLGSKDN
Sbjct: 181 LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHQNHLEHEEEEVYTLTKQEGASLGSKDN 240

Query: 241 SSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALATTFSM 300
           SSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALATTFSM
Sbjct: 241 SSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALATTFSM 300

Query: 301 LRHTAVILMEGTPREVHIESLENDIKNIKGVYDLHDLHIWSITVGKVVLSCHVVAEAGVC 360
           LRHTAVILMEGTPREVHIESLENDIKN+KGVYDLHDLHIWSITVGKVVLSCHVVAEAGVC
Sbjct: 301 LRHTAVILMEGTPREVHIESLENDIKNMKGVYDLHDLHIWSITVGKVVLSCHVVAEAGVC 360

Query: 361 SRELILKIKSHCEKRYNIVHTTIQVE 387
           SRELILKIKSHCEKRYNIVHTTIQVE
Sbjct: 361 SRELILKIKSHCEKRYNIVHTTIQVE 386

[Close]

BLAST of CSPI01G23400 vs. ExPASy TrEMBL
Match: A0A0A0M0L3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G525270 PE=4 SV=1)

HSP 1 Score: 740.7 bits (1911), Expect = 3.0e-210
Identity = 384/386 (99.48%), Postives = 385/386 (99.74%), Query Frame = 0


Query: 1   MGEEEVLILKTEHSDEINIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKR 60
           MGEEEVLILKTEHSDEINIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKR
Sbjct: 1   MGEEEVLILKTEHSDEINIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKR 60

Query: 61  SKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120
           SKSAMKLGGLILFYTIAIVVEIIGG RANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE
Sbjct: 61  SKSAMKLGGLILFYTIAIVVEIIGGFRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120

Query: 121 ATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFL 180
           ATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFL
Sbjct: 121 ATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFL 180

Query: 181 LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHQNHLEHEEEEVYTLTKQEGASLGSKDN 240
           LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHQNHLEHEEEEVYTLTKQEGASLGSKDN
Sbjct: 181 LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHQNHLEHEEEEVYTLTKQEGASLGSKDN 240

Query: 241 SSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALATTFSM 300
           SSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALATTFSM
Sbjct: 241 SSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALATTFSM 300

Query: 301 LRHTAVILMEGTPREVHIESLENDIKNIKGVYDLHDLHIWSITVGKVVLSCHVVAEAGVC 360
           LRHTAVILMEGTPREVHIESLENDIKN+KGVYDLHDLHIWSITVGKVVLSCHVVAEAGVC
Sbjct: 301 LRHTAVILMEGTPREVHIESLENDIKNMKGVYDLHDLHIWSITVGKVVLSCHVVAEAGVC 360

Query: 361 SRELILKIKSHCEKRYNIVHTTIQVE 387
           SRELILKIKSHCEKRYNIVHTTIQVE
Sbjct: 361 SRELILKIKSHCEKRYNIVHTTIQVE 386

[Close]

BLAST of CSPI01G23400 vs. ExPASy TrEMBL
Match: A0A1S3C537 (metal tolerance protein B OS=Cucumis melo OX=3656 GN=LOC103497126 PE=4 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 1.2e-195
Identity = 365/392 (93.11%), Postives = 375/392 (95.66%), Query Frame = 0


Query: 1   MGEEEVLILKTEHSDEINIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKR 60
           M EEEVLILKTEH DE+ +PIVAKK N VIP  TSSEVKCC+SGCAFSRLEHSNLESLKR
Sbjct: 1   MAEEEVLILKTEHWDEVKMPIVAKKRNVVIP--TSSEVKCCNSGCAFSRLEHSNLESLKR 60

Query: 61  SKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120
           SKSAMKLGG+ILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE
Sbjct: 61  SKSAMKLGGMILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120

Query: 121 ATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFL 180
           ATPQHSFGYNRLEVLGALVSVQLIWLISG+LIYEAIDRILA KTKVDGFLMFAVAAFGF+
Sbjct: 121 ATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFI 180

Query: 181 LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSH--QNHL----EHEEEEVYTLTKQEGAS 240
           LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSH  QNHL    +HE+EE+YTLTK EGAS
Sbjct: 181 LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTKLEGAS 240

Query: 241 LGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLAL 300
           LGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAG VLWFKPEWIVVDLICTLVFSVLAL
Sbjct: 241 LGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGFVLWFKPEWIVVDLICTLVFSVLAL 300

Query: 301 ATTFSMLRHTAVILMEGTPREVHIESLENDIKNIKGVYDLHDLHIWSITVGKVVLSCHVV 360
           ATTFSMLRH AVILMEGTPREVHIESLENDIKNIKGV+DLHDLHIWSITVGKVVLSCHVV
Sbjct: 301 ATTFSMLRHIAVILMEGTPREVHIESLENDIKNIKGVHDLHDLHIWSITVGKVVLSCHVV 360

Query: 361 AEAGVCSRELILKIKSHCEKRYNIVHTTIQVE 387
           AEAGVCSRELILKIKSHCEKRYNIVHTTIQVE
Sbjct: 361 AEAGVCSRELILKIKSHCEKRYNIVHTTIQVE 390

[Close]

BLAST of CSPI01G23400 vs. ExPASy TrEMBL
Match: A0A5D3BGQ9 (Metal tolerance protein B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001790 PE=4 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 1.2e-195
Identity = 365/392 (93.11%), Postives = 375/392 (95.66%), Query Frame = 0


Query: 1   MGEEEVLILKTEHSDEINIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKR 60
           M EEEVLILKTEH DE+ +PIVAKK N VIP  TSSEVKCC+SGCAFSRLEHSNLESLKR
Sbjct: 1   MAEEEVLILKTEHWDEVKMPIVAKKRNVVIP--TSSEVKCCNSGCAFSRLEHSNLESLKR 60

Query: 61  SKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120
           SKSAMKLGG+ILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE
Sbjct: 61  SKSAMKLGGMILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120

Query: 121 ATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFL 180
           ATPQHSFGYNRLEVLGALVSVQLIWLISG+LIYEAIDRILA KTKVDGFLMFAVAAFGF+
Sbjct: 121 ATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFI 180

Query: 181 LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSH--QNHL----EHEEEEVYTLTKQEGAS 240
           LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSH  QNHL    +HE+EE+YTLTK EGAS
Sbjct: 181 LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTKLEGAS 240

Query: 241 LGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLAL 300
           LGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAG VLWFKPEWIVVDLICTLVFSVLAL
Sbjct: 241 LGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGFVLWFKPEWIVVDLICTLVFSVLAL 300

Query: 301 ATTFSMLRHTAVILMEGTPREVHIESLENDIKNIKGVYDLHDLHIWSITVGKVVLSCHVV 360
           ATTFSMLRH AVILMEGTPREVHIESLENDIKNIKGV+DLHDLHIWSITVGKVVLSCHVV
Sbjct: 301 ATTFSMLRHIAVILMEGTPREVHIESLENDIKNIKGVHDLHDLHIWSITVGKVVLSCHVV 360

Query: 361 AEAGVCSRELILKIKSHCEKRYNIVHTTIQVE 387
           AEAGVCSRELILKIKSHCEKRYNIVHTTIQVE
Sbjct: 361 AEAGVCSRELILKIKSHCEKRYNIVHTTIQVE 390

[Close]

BLAST of CSPI01G23400 vs. ExPASy TrEMBL
Match: A0A5A7SNE8 (Metal tolerance protein B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold848G00010 PE=4 SV=1)

HSP 1 Score: 688.0 bits (1774), Expect = 2.3e-194
Identity = 362/392 (92.35%), Postives = 373/392 (95.15%), Query Frame = 0


Query: 1   MGEEEVLILKTEHSDEINIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKR 60
           M EEEVLILKTEH DE+ +PIVAKK N VIP  TSSEVKCC+SGCAFSRLEHSNLESLKR
Sbjct: 1   MAEEEVLILKTEHWDEVKMPIVAKKRNVVIP--TSSEVKCCNSGCAFSRLEHSNLESLKR 60

Query: 61  SKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120
           SKSAMKLGG+ILFYTIAIVVEIIGG RANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE
Sbjct: 61  SKSAMKLGGMILFYTIAIVVEIIGGFRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120

Query: 121 ATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFL 180
            TPQHSFGYNRLEVLGALVSVQLIWLISG+LIYEAIDRILA KTKVDGFLMFAVAAFGF+
Sbjct: 121 VTPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFI 180

Query: 181 LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSH--QNHL----EHEEEEVYTLTKQEGAS 240
           LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSH  QNHL    +HE+EE+YTLTK EGAS
Sbjct: 181 LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTKLEGAS 240

Query: 241 LGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLAL 300
           LGSKDNSSTLNINLQGAYLHVITD+IQSIGVMIAG VLWFKPEWIVVDLICTLVFSVLAL
Sbjct: 241 LGSKDNSSTLNINLQGAYLHVITDLIQSIGVMIAGFVLWFKPEWIVVDLICTLVFSVLAL 300

Query: 301 ATTFSMLRHTAVILMEGTPREVHIESLENDIKNIKGVYDLHDLHIWSITVGKVVLSCHVV 360
           ATTFSMLRH AVILMEGTPREVHIESLENDIKNIKGV+DLHDLHIWSITVGKVVLSCHVV
Sbjct: 301 ATTFSMLRHIAVILMEGTPREVHIESLENDIKNIKGVHDLHDLHIWSITVGKVVLSCHVV 360

Query: 361 AEAGVCSRELILKIKSHCEKRYNIVHTTIQVE 387
           AEAGVCSRELILKIKSHCEKRYNIVHTTIQVE
Sbjct: 361 AEAGVCSRELILKIKSHCEKRYNIVHTTIQVE 390

[Close]

BLAST of CSPI01G23400 vs. NCBI nr
Match: NP_001295861.1 (metal tolerance protein B [Cucumis sativus] >XP_004145281.2 metal tolerance protein B isoform X1 [Cucumis sativus] >XP_011658317.2 metal tolerance protein B isoform X1 [Cucumis sativus] >AFJ24701.1 metal transport protein 4 [Cucumis sativus] >KAE8653262.1 hypothetical protein Csa_019821 [Cucumis sativus])

HSP 1 Score: 742.3 bits (1915), Expect = 2.2e-210
Identity = 385/386 (99.74%), Postives = 386/386 (100.00%), Query Frame = 0


Query: 1   MGEEEVLILKTEHSDEINIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKR 60
           MGEEEVLILKTEHSDEINIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKR
Sbjct: 1   MGEEEVLILKTEHSDEINIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKR 60

Query: 61  SKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120
           SKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE
Sbjct: 61  SKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120

Query: 121 ATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFL 180
           ATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFL
Sbjct: 121 ATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFL 180

Query: 181 LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHQNHLEHEEEEVYTLTKQEGASLGSKDN 240
           LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHQNHLEHEEEEVYTLTKQEGASLGSKDN
Sbjct: 181 LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHQNHLEHEEEEVYTLTKQEGASLGSKDN 240

Query: 241 SSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALATTFSM 300
           SSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALATTFSM
Sbjct: 241 SSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALATTFSM 300

Query: 301 LRHTAVILMEGTPREVHIESLENDIKNIKGVYDLHDLHIWSITVGKVVLSCHVVAEAGVC 360
           LRHTAVILMEGTPREVHIESLENDIKN+KGVYDLHDLHIWSITVGKVVLSCHVVAEAGVC
Sbjct: 301 LRHTAVILMEGTPREVHIESLENDIKNMKGVYDLHDLHIWSITVGKVVLSCHVVAEAGVC 360

Query: 361 SRELILKIKSHCEKRYNIVHTTIQVE 387
           SRELILKIKSHCEKRYNIVHTTIQVE
Sbjct: 361 SRELILKIKSHCEKRYNIVHTTIQVE 386

[Close]

BLAST of CSPI01G23400 vs. NCBI nr
Match: XP_031741134.1 (metal tolerance protein B-like [Cucumis sativus] >XP_031741135.1 metal tolerance protein B-like [Cucumis sativus] >KAE8653268.1 hypothetical protein Csa_023325 [Cucumis sativus])

HSP 1 Score: 735.7 bits (1898), Expect = 2.0e-208
Identity = 384/386 (99.48%), Postives = 385/386 (99.74%), Query Frame = 0


Query: 1   MGEEEVLILKTEHSDEINIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKR 60
           MGEEEVLILKTEHSDEINIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKR
Sbjct: 1   MGEEEVLILKTEHSDEINIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKR 60

Query: 61  SKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120
           SKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE
Sbjct: 61  SKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120

Query: 121 ATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFL 180
           ATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFL
Sbjct: 121 ATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFL 180

Query: 181 LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHQNHLEHEEEEVYTLTKQEGASLGSKDN 240
           LNLFMVIWLGHS HHHHSHSSHCCHHDHHSHSHQNHLEHEEEEVYTLTKQEGASLGSKDN
Sbjct: 181 LNLFMVIWLGHS-HHHHSHSSHCCHHDHHSHSHQNHLEHEEEEVYTLTKQEGASLGSKDN 240

Query: 241 SSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALATTFSM 300
           SSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALATTFSM
Sbjct: 241 SSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALATTFSM 300

Query: 301 LRHTAVILMEGTPREVHIESLENDIKNIKGVYDLHDLHIWSITVGKVVLSCHVVAEAGVC 360
           LRHTAVILMEGTPREVHIESLENDIKN+KGVYDLHDLHIWSITVGKVVLSCHVVAEAGVC
Sbjct: 301 LRHTAVILMEGTPREVHIESLENDIKNMKGVYDLHDLHIWSITVGKVVLSCHVVAEAGVC 360

Query: 361 SRELILKIKSHCEKRYNIVHTTIQVE 387
           SRELILKIKSHCEKRYNIVHTTIQVE
Sbjct: 361 SRELILKIKSHCEKRYNIVHTTIQVE 385

[Close]

BLAST of CSPI01G23400 vs. NCBI nr
Match: TYJ97368.1 (metal tolerance protein B [Cucumis melo var. makuwa])

HSP 1 Score: 692.2 bits (1785), Expect = 2.6e-195
Identity = 365/392 (93.11%), Postives = 375/392 (95.66%), Query Frame = 0


Query: 1   MGEEEVLILKTEHSDEINIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKR 60
           M EEEVLILKTEH DE+ +PIVAKK N VIP  TSSEVKCC+SGCAFSRLEHSNLESLKR
Sbjct: 1   MAEEEVLILKTEHWDEVKMPIVAKKRNVVIP--TSSEVKCCNSGCAFSRLEHSNLESLKR 60

Query: 61  SKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120
           SKSAMKLGG+ILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE
Sbjct: 61  SKSAMKLGGMILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120

Query: 121 ATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFL 180
           ATPQHSFGYNRLEVLGALVSVQLIWLISG+LIYEAIDRILA KTKVDGFLMFAVAAFGF+
Sbjct: 121 ATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFI 180

Query: 181 LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSH--QNHL----EHEEEEVYTLTKQEGAS 240
           LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSH  QNHL    +HE+EE+YTLTK EGAS
Sbjct: 181 LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTKLEGAS 240

Query: 241 LGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLAL 300
           LGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAG VLWFKPEWIVVDLICTLVFSVLAL
Sbjct: 241 LGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGFVLWFKPEWIVVDLICTLVFSVLAL 300

Query: 301 ATTFSMLRHTAVILMEGTPREVHIESLENDIKNIKGVYDLHDLHIWSITVGKVVLSCHVV 360
           ATTFSMLRH AVILMEGTPREVHIESLENDIKNIKGV+DLHDLHIWSITVGKVVLSCHVV
Sbjct: 301 ATTFSMLRHIAVILMEGTPREVHIESLENDIKNIKGVHDLHDLHIWSITVGKVVLSCHVV 360

Query: 361 AEAGVCSRELILKIKSHCEKRYNIVHTTIQVE 387
           AEAGVCSRELILKIKSHCEKRYNIVHTTIQVE
Sbjct: 361 AEAGVCSRELILKIKSHCEKRYNIVHTTIQVE 390

[Close]

BLAST of CSPI01G23400 vs. NCBI nr
Match: XP_008457438.1 (PREDICTED: metal tolerance protein B [Cucumis melo] >XP_008457439.1 PREDICTED: metal tolerance protein B [Cucumis melo] >XP_008457440.1 PREDICTED: metal tolerance protein B [Cucumis melo])

HSP 1 Score: 692.2 bits (1785), Expect = 2.6e-195
Identity = 365/392 (93.11%), Postives = 375/392 (95.66%), Query Frame = 0


Query: 1   MGEEEVLILKTEHSDEINIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKR 60
           M EEEVLILKTEH DE+ +PIVAKK N VIP  TSSEVKCC+SGCAFSRLEHSNLESLKR
Sbjct: 1   MAEEEVLILKTEHWDEVKMPIVAKKRNVVIP--TSSEVKCCNSGCAFSRLEHSNLESLKR 60

Query: 61  SKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120
           SKSAMKLGG+ILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE
Sbjct: 61  SKSAMKLGGMILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120

Query: 121 ATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFL 180
           ATPQHSFGYNRLEVLGALVSVQLIWLISG+LIYEAIDRILA KTKVDGFLMFAVAAFGF+
Sbjct: 121 ATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFI 180

Query: 181 LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSH--QNHL----EHEEEEVYTLTKQEGAS 240
           LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSH  QNHL    +HE+EE+YTLTK EGAS
Sbjct: 181 LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTKLEGAS 240

Query: 241 LGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLAL 300
           LGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAG VLWFKPEWIVVDLICTLVFSVLAL
Sbjct: 241 LGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGFVLWFKPEWIVVDLICTLVFSVLAL 300

Query: 301 ATTFSMLRHTAVILMEGTPREVHIESLENDIKNIKGVYDLHDLHIWSITVGKVVLSCHVV 360
           ATTFSMLRH AVILMEGTPREVHIESLENDIKNIKGV+DLHDLHIWSITVGKVVLSCHVV
Sbjct: 301 ATTFSMLRHIAVILMEGTPREVHIESLENDIKNIKGVHDLHDLHIWSITVGKVVLSCHVV 360

Query: 361 AEAGVCSRELILKIKSHCEKRYNIVHTTIQVE 387
           AEAGVCSRELILKIKSHCEKRYNIVHTTIQVE
Sbjct: 361 AEAGVCSRELILKIKSHCEKRYNIVHTTIQVE 390

[Close]

BLAST of CSPI01G23400 vs. NCBI nr
Match: KAA0031763.1 (metal tolerance protein B [Cucumis melo var. makuwa])

HSP 1 Score: 688.0 bits (1774), Expect = 4.8e-194
Identity = 362/392 (92.35%), Postives = 373/392 (95.15%), Query Frame = 0


Query: 1   MGEEEVLILKTEHSDEINIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKR 60
           M EEEVLILKTEH DE+ +PIVAKK N VIP  TSSEVKCC+SGCAFSRLEHSNLESLKR
Sbjct: 1   MAEEEVLILKTEHWDEVKMPIVAKKRNVVIP--TSSEVKCCNSGCAFSRLEHSNLESLKR 60

Query: 61  SKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120
           SKSAMKLGG+ILFYTIAIVVEIIGG RANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE
Sbjct: 61  SKSAMKLGGMILFYTIAIVVEIIGGFRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120

Query: 121 ATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFL 180
            TPQHSFGYNRLEVLGALVSVQLIWLISG+LIYEAIDRILA KTKVDGFLMFAVAAFGF+
Sbjct: 121 VTPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFI 180

Query: 181 LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSH--QNHL----EHEEEEVYTLTKQEGAS 240
           LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSH  QNHL    +HE+EE+YTLTK EGAS
Sbjct: 181 LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTKLEGAS 240

Query: 241 LGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLAL 300
           LGSKDNSSTLNINLQGAYLHVITD+IQSIGVMIAG VLWFKPEWIVVDLICTLVFSVLAL
Sbjct: 241 LGSKDNSSTLNINLQGAYLHVITDLIQSIGVMIAGFVLWFKPEWIVVDLICTLVFSVLAL 300

Query: 301 ATTFSMLRHTAVILMEGTPREVHIESLENDIKNIKGVYDLHDLHIWSITVGKVVLSCHVV 360
           ATTFSMLRH AVILMEGTPREVHIESLENDIKNIKGV+DLHDLHIWSITVGKVVLSCHVV
Sbjct: 301 ATTFSMLRHIAVILMEGTPREVHIESLENDIKNIKGVHDLHDLHIWSITVGKVVLSCHVV 360

Query: 361 AEAGVCSRELILKIKSHCEKRYNIVHTTIQVE 387
           AEAGVCSRELILKIKSHCEKRYNIVHTTIQVE
Sbjct: 361 AEAGVCSRELILKIKSHCEKRYNIVHTTIQVE 390

[Close]

BLAST of CSPI01G23400 vs. TAIR 10
Match: AT2G29410.1 (metal tolerance protein B1 )

HSP 1 Score: 360.9 bits (925), Expect = 1.3e-99
Identity = 190/384 (49.48%), Postives = 260/384 (67.71%), Query Frame = 0


Query: 4   EEVLILKTEHSDEINIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKRSKS 63
           E++ ILK +  +E+  P          P+ T   +      CAF+R EH   E+ +R +S
Sbjct: 4   EQICILKPDDEEEMESP---------SPSKTEENLGVVPLSCAFTRQEHCVSETKEREES 63

Query: 64  AMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATP 123
             +L  LI  Y I + V+I+GG +ANSL+VMTDAAHLLSDVAG  VSL A+ VS WEA P
Sbjct: 64  TRRLSSLIFLYLIVMSVQIVGGFKANSLAVMTDAAHLLSDVAGLCVSLLAIKVSSWEANP 123

Query: 124 QHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFLLNL 183
           ++SFG+ RLEVL A +SVQLIWL+SG++I+EAI R+L+   +V+G +MF ++AFGF +NL
Sbjct: 124 RNSFGFKRLEVLAAFLSVQLIWLVSGVIIHEAIQRLLSRSREVNGEIMFGISAFGFFMNL 183

Query: 184 FMVIWLGHSH-HHHHSHSSHCCHHDHHSHSHQNHLEHEEEEVYTLTKQEGASLGSKDNSS 243
            MV+WLGH+H HHHH H     HH HH+H HQ+   H+E       ++     G K +S 
Sbjct: 184 VMVLWLGHNHSHHHHDH-----HHHHHNHKHQHQHHHKEVVAEEEEEEMNPLKGEKSSSK 243

Query: 244 TLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALATTFSMLR 303
            +NIN+QGAYLH + DMIQS+GVMI G ++W KP+W++VDLICTLVFS  ALA T  +L+
Sbjct: 244 EMNINIQGAYLHAMADMIQSLGVMIGGGIIWVKPKWVLVDLICTLVFSAFALAATLPILK 303

Query: 304 HTAVILMEGTPREVHIESLENDIKNIKGVYDLHDLHIWSITVGKVVLSCHVVAEAGVCSR 363
           +   ILME  PR++ IE LE  +K I GV  ++DLH+W ITVG++VLSCH++ E G   +
Sbjct: 304 NIFGILMERVPRDMDIEKLERGLKRIDGVKIVYDLHVWEITVGRIVLSCHILPEPGASPK 363

Query: 364 ELILKIKSHCEKRYNIVHTTIQVE 387
           E+I  +++ C K Y I H T+QVE
Sbjct: 364 EIITGVRNFCRKSYGIYHATVQVE 373

[Close]

BLAST of CSPI01G23400 vs. TAIR 10
Match: AT2G46800.1 (zinc transporter of Arabidopsis thaliana )

HSP 1 Score: 312.8 bits (800), Expect = 3.9e-85
Identity = 168/367 (45.78%), Postives = 244/367 (66.49%), Query Frame = 0


Query: 40  CCSSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAH 99
           C  + C FS  ++++ ++ +RS S  KL   ++   + + VE++GG++ANSL+++TDAAH
Sbjct: 31  CGEAPCGFSDSKNASGDAHERSASMRKLCIAVVLCLVFMSVEVVGGIKANSLAILTDAAH 90

Query: 100 LLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRI 159
           LLSDVA F++SLF++W +GWEATP+ ++G+ R+E+LGALVS+QLIWL++GIL+YEAI RI
Sbjct: 91  LLSDVAAFAISLFSLWAAGWEATPRQTYGFFRIEILGALVSIQLIWLLTGILVYEAIIRI 150

Query: 160 LAPKTKVDGFLMFAVAAFGFLLNLFMVIWLGHSH-------------HHHHSH----SSH 219
           +   ++V+GFLMF VAAFG ++N+ M + LGH H             HH+HSH    ++H
Sbjct: 151 VTETSEVNGFLMFLVAAFGLVVNIIMAVLLGHDHGHSHGHGHGHGHDHHNHSHGVTVTTH 210

Query: 220 CCHHDH---HSHSHQNHLEHEEEEVYTLTKQEGASLGSKDNSSTLNINLQGAYLHVITDM 279
             HHDH   HSH H     H   +V      +  +  +       NINLQGAYLHV+ D 
Sbjct: 211 HHHHDHEHGHSHGHGEDKHHAHGDVTEQLLDKSKTQVAAKEKRKRNINLQGAYLHVLGDS 270

Query: 280 IQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALATTFSMLRHTAVILMEGTPREVHIE 339
           IQS+GVMI G ++W+ PEW +VDLICTL FSV+ L TT +M+R+   +LME TPRE+   
Sbjct: 271 IQSVGVMIGGAIIWYNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTPREIDAT 330

Query: 340 SLENDIKNIKGVYDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCEKRYNIV 387
            LE  +  ++ V  +H+LHIW+ITVGKV+L+CHV       +  ++ K+  +  + YNI 
Sbjct: 331 KLEKGLLEMEEVVAVHELHIWAITVGKVLLACHVNIRPEADADMVLNKVIDYIRREYNIS 390

[Close]

BLAST of CSPI01G23400 vs. TAIR 10
Match: AT2G46800.2 (zinc transporter of Arabidopsis thaliana )

HSP 1 Score: 312.8 bits (800), Expect = 3.9e-85
Identity = 168/367 (45.78%), Postives = 244/367 (66.49%), Query Frame = 0


Query: 40  CCSSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAH 99
           C  + C FS  ++++ ++ +RS S  KL   ++   + + VE++GG++ANSL+++TDAAH
Sbjct: 31  CGEAPCGFSDSKNASGDAHERSASMRKLCIAVVLCLVFMSVEVVGGIKANSLAILTDAAH 90

Query: 100 LLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRI 159
           LLSDVA F++SLF++W +GWEATP+ ++G+ R+E+LGALVS+QLIWL++GIL+YEAI RI
Sbjct: 91  LLSDVAAFAISLFSLWAAGWEATPRQTYGFFRIEILGALVSIQLIWLLTGILVYEAIIRI 150

Query: 160 LAPKTKVDGFLMFAVAAFGFLLNLFMVIWLGHSH-------------HHHHSH----SSH 219
           +   ++V+GFLMF VAAFG ++N+ M + LGH H             HH+HSH    ++H
Sbjct: 151 VTETSEVNGFLMFLVAAFGLVVNIIMAVLLGHDHGHSHGHGHGHGHDHHNHSHGVTVTTH 210

Query: 220 CCHHDH---HSHSHQNHLEHEEEEVYTLTKQEGASLGSKDNSSTLNINLQGAYLHVITDM 279
             HHDH   HSH H     H   +V      +  +  +       NINLQGAYLHV+ D 
Sbjct: 211 HHHHDHEHGHSHGHGEDKHHAHGDVTEQLLDKSKTQVAAKEKRKRNINLQGAYLHVLGDS 270

Query: 280 IQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALATTFSMLRHTAVILMEGTPREVHIE 339
           IQS+GVMI G ++W+ PEW +VDLICTL FSV+ L TT +M+R+   +LME TPRE+   
Sbjct: 271 IQSVGVMIGGAIIWYNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTPREIDAT 330

Query: 340 SLENDIKNIKGVYDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCEKRYNIV 387
            LE  +  ++ V  +H+LHIW+ITVGKV+L+CHV       +  ++ K+  +  + YNI 
Sbjct: 331 KLEKGLLEMEEVVAVHELHIWAITVGKVLLACHVNIRPEADADMVLNKVIDYIRREYNIS 390

[Close]

BLAST of CSPI01G23400 vs. TAIR 10
Match: AT3G58810.1 (metal tolerance protein A2 )

HSP 1 Score: 306.2 bits (783), Expect = 3.7e-83
Identity = 167/348 (47.99%), Postives = 236/348 (67.82%), Query Frame = 0


Query: 40  CCSSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAH 99
           C  + C FS  + S++E+ +R+ S  KL   +L   I IVVE++GG++ANSL+++TDAAH
Sbjct: 86  CGEAPCGFSDAKTSSIEAQERAASMRKLLIAVLLCAIFIVVEVVGGIKANSLAILTDAAH 145

Query: 100 LLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRI 159
           LLSDVA F++SLF++W SGW+A PQ S+G+ R+E+LGALVS+Q+IWL++GIL+YEAI R+
Sbjct: 146 LLSDVAAFAISLFSLWASGWKANPQQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRL 205

Query: 160 LAPKTKVDGFLMFAVAAFGFLLNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHQ-NHLE 219
                +V+G LMFAV+A G L+N+ M I LGH H H H HS    H   H H H     E
Sbjct: 206 NNGSGEVEGSLMFAVSAVGLLVNIAMAILLGHDHGHGHGHSHDNGHGHSHDHGHGIAATE 265

Query: 220 HEEEEVYTLTKQEGASLGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEW 279
           H  +  +  ++     +  K      N+N+QGAYLHV+ D IQS+GVMI G ++W+KPEW
Sbjct: 266 HHHDSGHDESQLSDVLIEQKKQR---NVNIQGAYLHVLGDSIQSVGVMIGGAIIWYKPEW 325

Query: 280 IVVDLICTLVFSVLALATTFSMLRHTAVILMEGTPREVHIESLENDIKNIKGVYDLHDLH 339
            ++DLICTLVFSV+ L TT  MLR+   +LME TPRE+    LE  +  I+ V  +H+LH
Sbjct: 326 KILDLICTLVFSVIVLGTTIGMLRNILEVLMESTPREIDPTMLEKGVCEIEEVVAVHELH 385

Query: 340 IWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCEKRYNIVHTTIQVE 387
           IW+ITVGK++L+CHV       +  ++ KI  + ++ +NI H TIQ+E
Sbjct: 386 IWAITVGKLLLACHVKIRPEAEADMVLDKIIDYIKREHNISHVTIQIE 430

[Close]

BLAST of CSPI01G23400 vs. TAIR 10
Match: AT3G58810.2 (metal tolerance protein A2 )

HSP 1 Score: 306.2 bits (783), Expect = 3.7e-83
Identity = 167/348 (47.99%), Postives = 236/348 (67.82%), Query Frame = 0


Query: 40  CCSSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAH 99
           C  + C FS  + S++E+ +R+ S  KL   +L   I IVVE++GG++ANSL+++TDAAH
Sbjct: 47  CGEAPCGFSDAKTSSIEAQERAASMRKLLIAVLLCAIFIVVEVVGGIKANSLAILTDAAH 106

Query: 100 LLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRI 159
           LLSDVA F++SLF++W SGW+A PQ S+G+ R+E+LGALVS+Q+IWL++GIL+YEAI R+
Sbjct: 107 LLSDVAAFAISLFSLWASGWKANPQQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRL 166

Query: 160 LAPKTKVDGFLMFAVAAFGFLLNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHQ-NHLE 219
                +V+G LMFAV+A G L+N+ M I LGH H H H HS    H   H H H     E
Sbjct: 167 NNGSGEVEGSLMFAVSAVGLLVNIAMAILLGHDHGHGHGHSHDNGHGHSHDHGHGIAATE 226

Query: 220 HEEEEVYTLTKQEGASLGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEW 279
           H  +  +  ++     +  K      N+N+QGAYLHV+ D IQS+GVMI G ++W+KPEW
Sbjct: 227 HHHDSGHDESQLSDVLIEQKKQR---NVNIQGAYLHVLGDSIQSVGVMIGGAIIWYKPEW 286

Query: 280 IVVDLICTLVFSVLALATTFSMLRHTAVILMEGTPREVHIESLENDIKNIKGVYDLHDLH 339
            ++DLICTLVFSV+ L TT  MLR+   +LME TPRE+    LE  +  I+ V  +H+LH
Sbjct: 287 KILDLICTLVFSVIVLGTTIGMLRNILEVLMESTPREIDPTMLEKGVCEIEEVVAVHELH 346

Query: 340 IWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCEKRYNIVHTTIQVE 387
           IW+ITVGK++L+CHV       +  ++ KI  + ++ +NI H TIQ+E
Sbjct: 347 IWAITVGKLLLACHVKIRPEAEADMVLDKIIDYIKREHNISHVTIQIE 391

The following BLAST results are available for this feature:

BLAST of CSPI01G23400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6DBM8	1.8e-98	49.48	Metal tolerance protein B OS=Arabidopsis thaliana OX=3702 GN=MTPB PE=2 SV=1	[more]
Q9ZT63	5.6e-84	45.78	Metal tolerance protein 1 OS=Arabidopsis thaliana OX=3702 GN=MTP1 PE=1 SV=2	[more]
Q688R1	2.1e-83	43.60	Metal tolerance protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP1 PE=2 ...	[more]
Q9LXS1	5.2e-82	47.99	Metal tolerance protein A2 OS=Arabidopsis thaliana OX=3702 GN=MTPA2 PE=2 SV=2	[more]
Q9M271	1.8e-74	43.39	Metal tolerance protein A1 OS=Arabidopsis thaliana OX=3702 GN=MTPA1 PE=2 SV=1	[more]

BLAST of CSPI01G23400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
I1ZI47	1.0e-210	99.74	Metal transport protein 4 OS=Cucumis sativus OX=3659 GN=MTP4 PE=2 SV=1	[more]
A0A0A0M0L3	3.0e-210	99.48	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G525270 PE=4 SV=1	[more]
A0A1S3C537	1.2e-195	93.11	metal tolerance protein B OS=Cucumis melo OX=3656 GN=LOC103497126 PE=4 SV=1	[more]
A0A5D3BGQ9	1.2e-195	93.11	Metal tolerance protein B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A5A7SNE8	2.3e-194	92.35	Metal tolerance protein B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo...	[more]

BLAST of CSPI01G23400 vs. NCBI nr


Match Name	E-value	Identity	Description
NP_001295861.1	2.2e-210	99.74	metal tolerance protein B [Cucumis sativus] >XP_004145281.2 metal tolerance prot...	[more]
XP_031741134.1	2.0e-208	99.48	metal tolerance protein B-like [Cucumis sativus] >XP_031741135.1 metal tolerance...	[more]
TYJ97368.1	2.6e-195	93.11	metal tolerance protein B [Cucumis melo var. makuwa]	[more]
XP_008457438.1	2.6e-195	93.11	PREDICTED: metal tolerance protein B [Cucumis melo] >XP_008457439.1 PREDICTED: m...	[more]
KAA0031763.1	4.8e-194	92.35	metal tolerance protein B [Cucumis melo var. makuwa]	[more]

BLAST of CSPI01G23400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G29410.1	1.3e-99	49.48	metal tolerance protein B1	[more]
AT2G46800.1	3.9e-85	45.78	zinc transporter of Arabidopsis thaliana	[more]
AT2G46800.2	3.9e-85	45.78	zinc transporter of Arabidopsis thaliana	[more]
AT3G58810.1	3.7e-83	47.99	metal tolerance protein A2	[more]
AT3G58810.2	3.7e-83	47.99	metal tolerance protein A2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002524	Cation efflux protein	PFAM	PF01545	Cation_efflux	coord: 71..309 e-value: 4.2E-41 score: 140.9
IPR002524	Cation efflux protein	TIGRFAM	TIGR01297	TIGR01297	coord: 71..386 e-value: 1.2E-79 score: 265.5
IPR027469	Cation efflux transmembrane domain superfamily	GENE3D	1.20.1510.10	Cation efflux protein transmembrane domain	coord: 67..308 e-value: 5.0E-48 score: 165.3
IPR027469	Cation efflux transmembrane domain superfamily	SUPERFAMILY	161111	Cation efflux protein transmembrane domain-like	coord: 68..310
None	No IPR available	PANTHER	PTHR11562	CATION EFFLUX PROTEIN/ ZINC TRANSPORTER	coord: 5..386
None	No IPR available	PANTHER	PTHR11562:SF54	METAL TOLERANCE PROTEIN B	coord: 5..386
IPR036837	Cation efflux protein, cytoplasmic domain superfamily	SUPERFAMILY	160240	Cation efflux protein cytoplasmic domain-like	coord: 315..386

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G23400.1	CSPI01G23400.1	mRNA
CSPI01G23400.2	CSPI01G23400.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071577	zinc ion transmembrane transport
biological_process	GO:0006812	cation transport
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0005773	vacuole
molecular_function	GO:0005385	zinc ion transmembrane transporter activity
molecular_function	GO:0008324	cation transmembrane transporter activity