CSPI01G23220 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G23220
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionHaloacid dehalogenase-like hydrolase (HAD) superfamily protein
LocationChr1: 18862703 .. 18865612 (-)
RNA-Seq ExpressionCSPI01G23220
SyntenyCSPI01G23220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCGAATTCAAATTCATCATAGACGTTACTGATTATCGAAGGAAATTCTCCGCCCCCAGCGGCGCCGACCACCGCTGAGTACTGCATTCATTGCGGCGGAGCGATCTCCATTCTAACTCCACGCTCAACTCTTCAATTTTAAAATTCAATCAACCAAAAAAGGAAATTGCCAACTTTTTTTTATTGTAATTTAAAAGGCTTCGATTTCACTCACTCCCTCGATGACTGTTCATGAAAAGGCGTGAAAAGAACTGAGATCGTGTCTATATCTTATAAGTCCACGATATTGCATTGCATCCTCCCATTTCTTTTTCATTCTTCAACAACTTTTTGTGTATTCTCATTGGTAATTTCTCTTCTTCAGGCGGATTTCTCCATAGGCGGTTCTATTTGTATTTTTTGTGAGTATTGGTTTCCATTCTGATAGTTCATTTTTTAGCACCATTTGGAGAATACTTTCATGGGTTTTGGTTGTTTCGTTGGAATTTTTTTTTTCCAGTGATTTTGGGGTCAATCCACATTTTGTTATGACCATTTTCTTGTTTCATTTGCATGGGTTTGAGTTTGTTGAATCTAAATCAGTTGATAGATGAAATGCATTTTTTGTTTTTTTGTGGTTATTTTTGGGGGGATTTTGTTTAGTTTGAGATCTGTTTGATCAATGTTTTTGGGCATTGCAGATTGAAGTGTTCTTGAGTTTTGTGGGTACCTGGAAATGGAGAATGGTGATGAGTTCAATCAGATTTCTGATGCTAAATATGAATGTCTTCTCTTTGGTATTACATTTTGGACTTCTTATCTTTTTCAAGTTCAAAGTTGGATTCGAGAGTTTTTTATAACAAAGAATTATTTGTTTACTTTCAGATCTAGATGACACTCTTTATCCTTTTAACTCTGGTTTGGCAAAGGAAATCACCAAGAACATTCAAGGTTAGATGGAATTTCCTTTCCCCTTTCCCCTCTGGATTCAGAAACATTTGGGGCTGATAGTGTTTAGAGAAATGAGGCTCTTCGTATTTCTATGATATTGTCCATTTTGAACTTGAGTTTGCATGGATTTATCTTTTTGAAATGTTATCTTATGTGAATTGACATGGGATTATGTTCAAATCTCCAACTGATAACGAGTTAGAAGTTAGGTTTCTTTGTTGTTTTATTGATTGGATGAGTTTTTGTTACTTCTCTTGTAGAATATATGATTGAGAAGTTAGGAATGGAAGAGAACGTGCCTGAATTGTGCATTTCCTTGTACAAGATTTATGGGACAACAATGGCTGGTCTTAGAGTAAGTTAAAACATCATAGCCTTTTACATGATCTTTCTTCAAACCAACCATTGCAGTAAATTATTGGATACTATGTCCTGGTCGTTTTTCCTGGTTGCAGGCTATTGGGTATAACTTCGATTACGATGATTTCCATAGGTAACTAACTTTTTATCTACATTATTCTATGTTTGAGAATTTTGTGTTGTATTCACAATTCTTTTTCTTCCTGTTCTGCAGTTTTGTTCATGGAAGATTGCCATATGATATGCTCAAGCCTGACCCGTTATTGAGAAACCTACTTCACAGCTTACCAATTCGAAAATTTGTAAGTAGCTTGTTATGGAATTGACAAATGAAGAAAACAGAAAACATAAATCAATAGTGAATCAACACAGAGACATTTTAACCTCCCTTACCAGACTTTAGTTACACTATTTGGTGTCTATTTTAGTTTTAACTAGTGTTTTGATTTTTACCCTTTCTTTTTGTGTTTCTCTCTGATTTCTGACCTCAGATATTCACAAATGGTGATATGGCCCATGCAAACAGAGCGCTGAAGAGACTAGGATTAGAGGACTGCTTTGAAGGGATTTTATGCTTTGAGACTTTGAATCCTGATAAGGGAACGGTTGATGAGGAAGCAGGGTCTGTAATTTTTGACATCAATCAGTACATGAGCAATCCAAATTCTGATCTTGATCTACCGAAGACGCCAGTTGTCTGCAAACCATTCGAAGAGGCATATAAGCAAGTTTTCGAGATAGCCAATATCAACCCGAAAAAAACTGTAAGTTGAGAACAGATCAGAGTAACTTTATGTGACCTTTTCCAAGAGTTCGATGATTATAATTCTGTTGTGTTGAAAACAAAACATCCAATATTTAAAACTAGAATCAATAAACTTATTATTCATTTAATATATAAGATCTTAACAACTTACTGGGCCTGCCAAAAGCCATTCTTCTTAAGTGCCTAATACATTAACAAGAATAAATTTTAGCCTTTTAACTTGCAAAGCCTGCTATCTTGACCATCAAGAACATTGATCGTTTAATACTTAGCCTGATCTGTTATCTAACTCCATTTTCATTTCACAATACTACATTCTTCTTATTTTCTTGTTTCCAGTTGTTCTTTGATGATAGTGTTCGAAATCTACAAACGGGGAAGTTGGTGGGCCTTCACACAGTGCTTGTAAGTGGCAATACCATTGGGATTTCTTTAAAGCTTGCTGCTGAATTCACTTACATTCTAACCTAATCATGAATTCAGGTTGGAAATTCGCAACGAATCAAGGGGGTGGATCACGCTTTTGAGAGCATCCATAATATCAAGGAAGGATTGCCTGAGCTATGGGAAGACATGGAGAAGCTCAAGAGCGTCACATACTCCAGAAAGGAGGTAGCTATCGAGACTTCAGTTAGAGCATAATTATTAATTAGTATTTATGATCTTAACTTACAAGTGGACAGTGCAAAAACTGTTGGCAATTGCTGCAATGAATGCAAAATATGAGAACTTGTTAATAGAACAATATTATTGGCTCATGATAAGAGGGAAAATTTGTCTCCTTTTAGATTTATGTTGATCATAAACTCTTTCTTTCTTTCTTTCTTTCTTTCTTTTTAAGAGTTCAACATGAGG

mRNA sequence

CCGAATTCAAATTCATCATAGACGTTACTGATTATCGAAGGAAATTCTCCGCCCCCAGCGGCGCCGACCACCGCTGAGTACTGCATTCATTGCGGCGGAGCGATCTCCATTCTAACTCCACGCTCAACTCTTCAATTTTAAAATTCAATCAACCAAAAAAGGAAATTGCCAACTTTTTTTTATTGTAATTTAAAAGGCTTCGATTTCACTCACTCCCTCGATGACTGTTCATGAAAAGGCGTGAAAAGAACTGAGATCGTGTCTATATCTTATAAGTCCACGATATTGCATTGCATCCTCCCATTTCTTTTTCATTCTTCAACAACTTTTTGTGTATTCTCATTGGTAATTTCTCTTCTTCAGGCGGATTTCTCCATAGGCGGTTCTATTTGTATTTTTTATTGAAGTGTTCTTGAGTTTTGTGGGTACCTGGAAATGGAGAATGGTGATGAGTTCAATCAGATTTCTGATGCTAAATATGAATGTCTTCTCTTTGATCTAGATGACACTCTTTATCCTTTTAACTCTGGTTTGGCAAAGGAAATCACCAAGAACATTCAAGAATATATGATTGAGAAGTTAGGAATGGAAGAGAACGTGCCTGAATTGTGCATTTCCTTGTACAAGATTTATGGGACAACAATGGCTGGTCTTAGAGCTATTGGGTATAACTTCGATTACGATGATTTCCATAGTTTTGTTCATGGAAGATTGCCATATGATATGCTCAAGCCTGACCCGTTATTGAGAAACCTACTTCACAGCTTACCAATTCGAAAATTTATATTCACAAATGGTGATATGGCCCATGCAAACAGAGCGCTGAAGAGACTAGGATTAGAGGACTGCTTTGAAGGGATTTTATGCTTTGAGACTTTGAATCCTGATAAGGGAACGGTTGATGAGGAAGCAGGGTCTGTAATTTTTGACATCAATCAGTACATGAGCAATCCAAATTCTGATCTTGATCTACCGAAGACGCCAGTTGTCTGCAAACCATTCGAAGAGGCATATAAGCAAGTTTTCGAGATAGCCAATATCAACCCGAAAAAAACTTTGTTCTTTGATGATAGTGTTCGAAATCTACAAACGGGGAAGTTGGTGGGCCTTCACACAGTGCTTGTTGGAAATTCGCAACGAATCAAGGGGGTGGATCACGCTTTTGAGAGCATCCATAATATCAAGGAAGGATTGCCTGAGCTATGGGAAGACATGGAGAAGCTCAAGAGCGTCACATACTCCAGAAAGGAGGTAGCTATCGAGACTTCAGTTAGAGCATAATTATTAATTAGTATTTATGATCTTAACTTACAAGTGGACAGTGCAAAAACTGTTGGCAATTGCTGCAATGAATGCAAAATATGAGAACTTGTTAATAGAACAATATTATTGGCTCATGATAAGAGGGAAAATTTGTCTCCTTTTAGATTTATGTTGATCATAAACTCTTTCTTTCTTTCTTTCTTTCTTTCTTTTTAAGAGTTCAACATGAGG

Coding sequence (CDS)

ATGGAGAATGGTGATGAGTTCAATCAGATTTCTGATGCTAAATATGAATGTCTTCTCTTTGATCTAGATGACACTCTTTATCCTTTTAACTCTGGTTTGGCAAAGGAAATCACCAAGAACATTCAAGAATATATGATTGAGAAGTTAGGAATGGAAGAGAACGTGCCTGAATTGTGCATTTCCTTGTACAAGATTTATGGGACAACAATGGCTGGTCTTAGAGCTATTGGGTATAACTTCGATTACGATGATTTCCATAGTTTTGTTCATGGAAGATTGCCATATGATATGCTCAAGCCTGACCCGTTATTGAGAAACCTACTTCACAGCTTACCAATTCGAAAATTTATATTCACAAATGGTGATATGGCCCATGCAAACAGAGCGCTGAAGAGACTAGGATTAGAGGACTGCTTTGAAGGGATTTTATGCTTTGAGACTTTGAATCCTGATAAGGGAACGGTTGATGAGGAAGCAGGGTCTGTAATTTTTGACATCAATCAGTACATGAGCAATCCAAATTCTGATCTTGATCTACCGAAGACGCCAGTTGTCTGCAAACCATTCGAAGAGGCATATAAGCAAGTTTTCGAGATAGCCAATATCAACCCGAAAAAAACTTTGTTCTTTGATGATAGTGTTCGAAATCTACAAACGGGGAAGTTGGTGGGCCTTCACACAGTGCTTGTTGGAAATTCGCAACGAATCAAGGGGGTGGATCACGCTTTTGAGAGCATCCATAATATCAAGGAAGGATTGCCTGAGCTATGGGAAGACATGGAGAAGCTCAAGAGCGTCACATACTCCAGAAAGGAGGTAGCTATCGAGACTTCAGTTAGAGCATAA

Protein sequence

MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTNGDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEAGSVIFDINQYMSNPNSDLDLPKTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVDHAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA*
Homology
BLAST of CSPI01G23220 vs. ExPASy Swiss-Prot
Match: Q09893 (Uncharacterized protein C24B11.05 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC24B11.05 PE=3 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 4.7e-16
Identity = 74/251 (29.48%), Postives = 111/251 (44.22%), Query Frame = 0

Query: 18  LLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGM-EENVPELCISLYKIYGTTMAGLRAI 77
           + FDLD+ LYP +  +   +   I  +  +KLG+  E    L    Y+ YG  + GL  +
Sbjct: 8   IFFDLDNCLYPKSYKIHNMMAARITAFFSDKLGIPTEEAERLREVYYRHYGIAIRGL-VL 67

Query: 78  GYNFDYDDFHSFVHGRLPYD-MLKPDPLLRNLLHSLPIRK----FIFTNGDMAHANRALK 137
            +  D  D+   V   LP + ++K D +LR +L  L +RK    +IFTN  + HANR LK
Sbjct: 68  HHEIDAVDYDQRVDQSLPLEKVIKKDEVLREML--LELRKKYKCWIFTNAYIVHANRVLK 127

Query: 138 RLGLEDCFEGILCFETLNPDKGTVDEEAGSVIFDINQYMSNPNSDLDLPKTPVVCKPFEE 197
            LG+EDCF+GI                                +  D     ++ KP  E
Sbjct: 128 YLGIEDCFDGI--------------------------------TYCDYNAKDLIAKPMPE 187

Query: 198 AYKQVFEIANINPK-KTLFFDDSVRNLQTGKLVG-LHTV-LVGNSQRI---KGVDHAFES 257
            Y++V   A +  K K +F DDS  N+   +  G  +TV LV +   +   +   H    
Sbjct: 188 MYERVMREAGVTDKDKCIFVDDSYGNILGAREFGWKYTVQLVEHGDPLPQPQAGSHVIRD 223

BLAST of CSPI01G23220 vs. ExPASy Swiss-Prot
Match: P40025 (Phosphate metabolism protein 8 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PHM8 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 5.1e-10
Identity = 45/140 (32.14%), Postives = 74/140 (52.86%), Query Frame = 0

Query: 12  DAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI-SLYKIYGTTM 71
           D   +   FD+D+TLY  ++ +   + +++  +   +LG +++  E  I S Y+ YG ++
Sbjct: 49  DPARKVFFFDIDNTLYRKSTKVQLLMQQSLSNFFKYELGFDDDEAERLIESYYQEYGLSV 108

Query: 72  AGLRAIGYNFDYDDFHSFVHGRLP-YDMLKPDPLLRNLLHSLPIRK-------FIFTNGD 131
            GL       D   +++F+   LP  D LKPD  LR LL +L  +K       ++FTN  
Sbjct: 109 KGLIKNKQIDDVLQYNTFIDDSLPLQDYLKPDWKLRELLINLKKKKLGKFDKLWLFTNSY 168

Query: 132 MAHANRALKRLGLEDCFEGI 143
             HA R +K LG+ D F+GI
Sbjct: 169 KNHAIRCVKILGIADLFDGI 188

BLAST of CSPI01G23220 vs. ExPASy Swiss-Prot
Match: P53078 (Suppressor of disruption of TFIIS OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SDT1 PE=1 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 6.6e-10
Identity = 57/218 (26.15%), Postives = 100/218 (45.87%), Query Frame = 0

Query: 20  FDLDDTLYPFNSGLAKEITKNIQEYMIEKLGME-ENVPELCISLYKIYGTTMAGLRAIGY 79
           FD+D+ LY  ++ +   + ++I  +    L +  E+   L  S YK YG  + GL  + +
Sbjct: 60  FDIDNCLYKSSTRIHDLMQQSILRFFQTHLKLSPEDAHVLNNSYYKEYGLAIRGL-VMFH 119

Query: 80  NFDYDDFHSFVHGRLP-YDMLKPDPLLRNLLHSLP-----IRKFIFTNGDMAHANRALKR 139
             +  +++  V   LP  D+LKPD  LRN+L  L       + ++FTN    HA R L+ 
Sbjct: 120 KVNALEYNRLVDDSLPLQDILKPDIPLRNMLLRLRQSGKIDKLWLFTNAYKNHAIRCLRL 179

Query: 140 LGLEDCFEGILCFETLNPDKGTVDEEAGSVIFDINQYMSNPNSDLDLPKTPVVCKPFEEA 199
           LG+ D F+G+                          Y     +D       +VCKP  +A
Sbjct: 180 LGIADLFDGL-------------------------TYCDYSRTD------TLVCKPHVKA 239

Query: 200 YKQVFEIANI-NPKKTLFFDDSVRNLQTGKLVGLHTVL 230
           +++  + + +   +   F DDS +N++TG  +G+ T +
Sbjct: 240 FEKAMKESGLARYENAYFIDDSGKNIETGIKLGMKTCI 245

BLAST of CSPI01G23220 vs. ExPASy TrEMBL
Match: A0A0A0LVD2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G523620 PE=4 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 8.6e-162
Identity = 280/281 (99.64%), Postives = 280/281 (99.64%), Query Frame = 0

Query: 1   MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI 60
           MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI
Sbjct: 1   MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI 60

Query: 61  SLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTN 120
           SLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTN
Sbjct: 61  SLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTN 120

Query: 121 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEAGSVIFDINQYMSNPNSDLDLP 180
           GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEE GSVIFDINQYMSNPNSDLDLP
Sbjct: 121 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEGSVIFDINQYMSNPNSDLDLP 180

Query: 181 KTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVD 240
           KTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVD
Sbjct: 181 KTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVD 240

Query: 241 HAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 282
           HAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA
Sbjct: 241 HAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 281

BLAST of CSPI01G23220 vs. ExPASy TrEMBL
Match: A0A1S4E1J3 (uncharacterized protein C24B11.05-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497146 PE=4 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 6.3e-157
Identity = 274/282 (97.16%), Postives = 279/282 (98.94%), Query Frame = 0

Query: 1   MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI 60
           MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI
Sbjct: 1   MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI 60

Query: 61  SLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTN 120
           SLYKIYGTTMAGL+AIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRK IFTN
Sbjct: 61  SLYKIYGTTMAGLKAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKLIFTN 120

Query: 121 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVD-EEAGSVIFDINQYMSNPNSDLDL 180
           GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVD EEAGSVIFDINQYMSNPNSD+DL
Sbjct: 121 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEAGSVIFDINQYMSNPNSDIDL 180

Query: 181 PKTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGV 240
           PKTPVVCKPFEEA+KQVFE+ANINPKKTLFFDDSVRNLQTGKLVGLHTVL+GNSQRIKGV
Sbjct: 181 PKTPVVCKPFEEAFKQVFEMANINPKKTLFFDDSVRNLQTGKLVGLHTVLIGNSQRIKGV 240

Query: 241 DHAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 282
           DHAFESIHNIKEGLPELWEDMEKLKSVTYSRK VAIETSVRA
Sbjct: 241 DHAFESIHNIKEGLPELWEDMEKLKSVTYSRK-VAIETSVRA 281

BLAST of CSPI01G23220 vs. ExPASy TrEMBL
Match: A0A5D3BED8 (Haloacid dehalogenase-like hydrolase superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G002080 PE=4 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.2e-155
Identity = 272/280 (97.14%), Postives = 277/280 (98.93%), Query Frame = 0

Query: 3   NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL 62
           NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL
Sbjct: 199 NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL 258

Query: 63  YKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTNGD 122
           YKIYGTTMAGL+AIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRK IFTNGD
Sbjct: 259 YKIYGTTMAGLKAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKLIFTNGD 318

Query: 123 MAHANRALKRLGLEDCFEGILCFETLNPDKGTVD-EEAGSVIFDINQYMSNPNSDLDLPK 182
           MAHANRALKRLGLEDCFEGILCFETLNPDKGTVD EEAGSVIFDINQYMSNPNSD+DLPK
Sbjct: 319 MAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEAGSVIFDINQYMSNPNSDIDLPK 378

Query: 183 TPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVDH 242
           TPVVCKPFEEA+KQVFE+ANINPKKTLFFDDSVRNLQTGKLVGLHTVL+GNSQRIKGVDH
Sbjct: 379 TPVVCKPFEEAFKQVFEMANINPKKTLFFDDSVRNLQTGKLVGLHTVLIGNSQRIKGVDH 438

Query: 243 AFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 282
           AFESIHNIKEGLPELWEDMEKLKSVTYSRK VAIETSVRA
Sbjct: 439 AFESIHNIKEGLPELWEDMEKLKSVTYSRK-VAIETSVRA 477

BLAST of CSPI01G23220 vs. ExPASy TrEMBL
Match: A0A5A7VPY6 (Haloacid dehalogenase-like hydrolase superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold352G00750 PE=4 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.2e-155
Identity = 272/280 (97.14%), Postives = 277/280 (98.93%), Query Frame = 0

Query: 3   NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL 62
           NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL
Sbjct: 280 NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL 339

Query: 63  YKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTNGD 122
           YKIYGTTMAGL+AIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRK IFTNGD
Sbjct: 340 YKIYGTTMAGLKAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKLIFTNGD 399

Query: 123 MAHANRALKRLGLEDCFEGILCFETLNPDKGTVD-EEAGSVIFDINQYMSNPNSDLDLPK 182
           MAHANRALKRLGLEDCFEGILCFETLNPDKGTVD EEAGSVIFDINQYMSNPNSD+DLPK
Sbjct: 400 MAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEAGSVIFDINQYMSNPNSDIDLPK 459

Query: 183 TPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVDH 242
           TPVVCKPFEEA+KQVFE+ANINPKKTLFFDDSVRNLQTGKLVGLHTVL+GNSQRIKGVDH
Sbjct: 460 TPVVCKPFEEAFKQVFEMANINPKKTLFFDDSVRNLQTGKLVGLHTVLIGNSQRIKGVDH 519

Query: 243 AFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 282
           AFESIHNIKEGLPELWEDMEKLKSVTYSRK VAIETSVRA
Sbjct: 520 AFESIHNIKEGLPELWEDMEKLKSVTYSRK-VAIETSVRA 558

BLAST of CSPI01G23220 vs. ExPASy TrEMBL
Match: A0A1S4E293 (uncharacterized protein C24B11.05-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103497146 PE=4 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 2.8e-144
Identity = 254/262 (96.95%), Postives = 259/262 (98.85%), Query Frame = 0

Query: 21  DLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISLYKIYGTTMAGLRAIGYNF 80
           DLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISLYKIYGTTMAGL+AIGYNF
Sbjct: 4   DLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISLYKIYGTTMAGLKAIGYNF 63

Query: 81  DYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTNGDMAHANRALKRLGLEDCFE 140
           DYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRK IFTNGDMAHANRALKRLGLEDCFE
Sbjct: 64  DYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKLIFTNGDMAHANRALKRLGLEDCFE 123

Query: 141 GILCFETLNPDKGTVD-EEAGSVIFDINQYMSNPNSDLDLPKTPVVCKPFEEAYKQVFEI 200
           GILCFETLNPDKGTVD EEAGSVIFDINQYMSNPNSD+DLPKTPVVCKPFEEA+KQVFE+
Sbjct: 124 GILCFETLNPDKGTVDEEEAGSVIFDINQYMSNPNSDIDLPKTPVVCKPFEEAFKQVFEM 183

Query: 201 ANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVDHAFESIHNIKEGLPELWED 260
           ANINPKKTLFFDDSVRNLQTGKLVGLHTVL+GNSQRIKGVDHAFESIHNIKEGLPELWED
Sbjct: 184 ANINPKKTLFFDDSVRNLQTGKLVGLHTVLIGNSQRIKGVDHAFESIHNIKEGLPELWED 243

Query: 261 MEKLKSVTYSRKEVAIETSVRA 282
           MEKLKSVTYSRK VAIETSVRA
Sbjct: 244 MEKLKSVTYSRK-VAIETSVRA 264

BLAST of CSPI01G23220 vs. NCBI nr
Match: XP_011658227.1 (uncharacterized protein C24B11.05 [Cucumis sativus] >XP_011658231.1 uncharacterized protein C24B11.05 [Cucumis sativus] >KGN65738.1 hypothetical protein Csa_019919 [Cucumis sativus])

HSP 1 Score: 579.3 bits (1492), Expect = 1.8e-161
Identity = 280/281 (99.64%), Postives = 280/281 (99.64%), Query Frame = 0

Query: 1   MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI 60
           MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI
Sbjct: 1   MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI 60

Query: 61  SLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTN 120
           SLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTN
Sbjct: 61  SLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTN 120

Query: 121 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEAGSVIFDINQYMSNPNSDLDLP 180
           GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEE GSVIFDINQYMSNPNSDLDLP
Sbjct: 121 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEGSVIFDINQYMSNPNSDLDLP 180

Query: 181 KTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVD 240
           KTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVD
Sbjct: 181 KTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVD 240

Query: 241 HAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 282
           HAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA
Sbjct: 241 HAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 281

BLAST of CSPI01G23220 vs. NCBI nr
Match: XP_016902092.1 (PREDICTED: uncharacterized protein C24B11.05-like isoform X1 [Cucumis melo] >XP_016902093.1 PREDICTED: uncharacterized protein C24B11.05-like isoform X1 [Cucumis melo] >XP_016902094.1 PREDICTED: uncharacterized protein C24B11.05-like isoform X1 [Cucumis melo])

HSP 1 Score: 563.1 bits (1450), Expect = 1.3e-156
Identity = 274/282 (97.16%), Postives = 279/282 (98.94%), Query Frame = 0

Query: 1   MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI 60
           MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI
Sbjct: 1   MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI 60

Query: 61  SLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTN 120
           SLYKIYGTTMAGL+AIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRK IFTN
Sbjct: 61  SLYKIYGTTMAGLKAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKLIFTN 120

Query: 121 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVD-EEAGSVIFDINQYMSNPNSDLDL 180
           GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVD EEAGSVIFDINQYMSNPNSD+DL
Sbjct: 121 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEAGSVIFDINQYMSNPNSDIDL 180

Query: 181 PKTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGV 240
           PKTPVVCKPFEEA+KQVFE+ANINPKKTLFFDDSVRNLQTGKLVGLHTVL+GNSQRIKGV
Sbjct: 181 PKTPVVCKPFEEAFKQVFEMANINPKKTLFFDDSVRNLQTGKLVGLHTVLIGNSQRIKGV 240

Query: 241 DHAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 282
           DHAFESIHNIKEGLPELWEDMEKLKSVTYSRK VAIETSVRA
Sbjct: 241 DHAFESIHNIKEGLPELWEDMEKLKSVTYSRK-VAIETSVRA 281

BLAST of CSPI01G23220 vs. NCBI nr
Match: KAA0067755.1 (Haloacid dehalogenase-like hydrolase superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 558.9 bits (1439), Expect = 2.5e-155
Identity = 272/280 (97.14%), Postives = 277/280 (98.93%), Query Frame = 0

Query: 3   NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL 62
           NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL
Sbjct: 280 NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL 339

Query: 63  YKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTNGD 122
           YKIYGTTMAGL+AIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRK IFTNGD
Sbjct: 340 YKIYGTTMAGLKAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKLIFTNGD 399

Query: 123 MAHANRALKRLGLEDCFEGILCFETLNPDKGTVD-EEAGSVIFDINQYMSNPNSDLDLPK 182
           MAHANRALKRLGLEDCFEGILCFETLNPDKGTVD EEAGSVIFDINQYMSNPNSD+DLPK
Sbjct: 400 MAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEAGSVIFDINQYMSNPNSDIDLPK 459

Query: 183 TPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVDH 242
           TPVVCKPFEEA+KQVFE+ANINPKKTLFFDDSVRNLQTGKLVGLHTVL+GNSQRIKGVDH
Sbjct: 460 TPVVCKPFEEAFKQVFEMANINPKKTLFFDDSVRNLQTGKLVGLHTVLIGNSQRIKGVDH 519

Query: 243 AFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 282
           AFESIHNIKEGLPELWEDMEKLKSVTYSRK VAIETSVRA
Sbjct: 520 AFESIHNIKEGLPELWEDMEKLKSVTYSRK-VAIETSVRA 558

BLAST of CSPI01G23220 vs. NCBI nr
Match: TYJ97389.1 (Haloacid dehalogenase-like hydrolase superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 558.9 bits (1439), Expect = 2.5e-155
Identity = 272/280 (97.14%), Postives = 277/280 (98.93%), Query Frame = 0

Query: 3   NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL 62
           NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL
Sbjct: 199 NGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCISL 258

Query: 63  YKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTNGD 122
           YKIYGTTMAGL+AIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRK IFTNGD
Sbjct: 259 YKIYGTTMAGLKAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKLIFTNGD 318

Query: 123 MAHANRALKRLGLEDCFEGILCFETLNPDKGTVD-EEAGSVIFDINQYMSNPNSDLDLPK 182
           MAHANRALKRLGLEDCFEGILCFETLNPDKGTVD EEAGSVIFDINQYMSNPNSD+DLPK
Sbjct: 319 MAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEEAGSVIFDINQYMSNPNSDIDLPK 378

Query: 183 TPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVDH 242
           TPVVCKPFEEA+KQVFE+ANINPKKTLFFDDSVRNLQTGKLVGLHTVL+GNSQRIKGVDH
Sbjct: 379 TPVVCKPFEEAFKQVFEMANINPKKTLFFDDSVRNLQTGKLVGLHTVLIGNSQRIKGVDH 438

Query: 243 AFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 282
           AFESIHNIKEGLPELWEDMEKLKSVTYSRK VAIETSVRA
Sbjct: 439 AFESIHNIKEGLPELWEDMEKLKSVTYSRK-VAIETSVRA 477

BLAST of CSPI01G23220 vs. NCBI nr
Match: XP_038893602.1 (uncharacterized protein LOC120082486 [Benincasa hispida] >XP_038893603.1 uncharacterized protein LOC120082486 [Benincasa hispida] >XP_038893604.1 uncharacterized protein LOC120082486 [Benincasa hispida])

HSP 1 Score: 551.6 bits (1420), Expect = 3.9e-153
Identity = 266/281 (94.66%), Postives = 274/281 (97.51%), Query Frame = 0

Query: 1   MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEENVPELCI 60
           MENGD+FNQISDAKYECLLFDLDDTLYPFNSGLA EITKNIQEYMIEKLGMEENVPELCI
Sbjct: 1   MENGDQFNQISDAKYECLLFDLDDTLYPFNSGLANEITKNIQEYMIEKLGMEENVPELCI 60

Query: 61  SLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTN 120
           SLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDP+LRNLLHSLPIRK IFTN
Sbjct: 61  SLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPVLRNLLHSLPIRKLIFTN 120

Query: 121 GDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEAGSVIFDINQYMSNPNSDLDLP 180
           GDMAHA RALKRLGLEDCFEGILCFETLNPDKGTVDEEAGSVIF+INQYMSNPNSD+DLP
Sbjct: 121 GDMAHATRALKRLGLEDCFEGILCFETLNPDKGTVDEEAGSVIFNINQYMSNPNSDIDLP 180

Query: 181 KTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVD 240
           KTPVVCKPFEEA+KQVFEIANINP+KTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVD
Sbjct: 181 KTPVVCKPFEEAFKQVFEIANINPQKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVD 240

Query: 241 HAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 282
           HAFESIHNIKEGLPELWE MEKL S++YS K VAIETSVRA
Sbjct: 241 HAFESIHNIKEGLPELWEAMEKLNSISYSEK-VAIETSVRA 280

BLAST of CSPI01G23220 vs. TAIR 10
Match: AT5G59480.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 375.2 bits (962), Expect = 4.7e-104
Identity = 180/277 (64.98%), Postives = 222/277 (80.14%), Query Frame = 0

Query: 5   DEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEEN-VPELCISLY 64
           + F Q S+AKY+CLLFD+DDTLYP +SGLA E+ KNIQEYM++KLG+EE+ V ELC+SLY
Sbjct: 5   EHFQQTSEAKYDCLLFDIDDTLYPLSSGLAMEVKKNIQEYMVQKLGIEEDKVQELCLSLY 64

Query: 65  KIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTNGDM 124
           KIYGTTMAGL+A+GY+FDYDDFH FVHGRLPY  LKPDP+LRN++ SLPIRK +FTN D 
Sbjct: 65  KIYGTTMAGLKAVGYDFDYDDFHRFVHGRLPYSTLKPDPILRNIILSLPIRKVVFTNADK 124

Query: 125 AHANRALKRLGLEDCFEGILCFETLNP---DKGTVDEEAGSVIFDINQYMSNPNSDLDLP 184
           AHA + + RLGLE CFE I+ FETLNP    +  VD +    IFDI  YM+NP+S ++LP
Sbjct: 125 AHAAKIIARLGLEGCFEKIISFETLNPITKTESPVDTKTRE-IFDIISYMANPDSSIELP 184

Query: 185 KTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVD 244
           KT VVCKP E A++QVF++ANINPKKTLFFDDS+RN+QTGK VGLHTV VG S R +GVD
Sbjct: 185 KTSVVCKPSEGAFEQVFKMANINPKKTLFFDDSIRNIQTGKRVGLHTVWVGTSHREEGVD 244

Query: 245 HAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIET 278
            A E IHNI+E LP+LW+ ++       +R++VAIET
Sbjct: 245 IALEHIHNIREALPQLWDAVDDKAKEIRTRQKVAIET 280

BLAST of CSPI01G23220 vs. TAIR 10
Match: AT5G59480.2 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 370.9 bits (951), Expect = 8.9e-103
Identity = 180/277 (64.98%), Postives = 222/277 (80.14%), Query Frame = 0

Query: 5   DEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEEN-VPELCISLY 64
           + F Q S+AKY+CLLFD+DDTLYP +SGLA E+ KNIQEYM++KLG+EE+ V ELC+SLY
Sbjct: 5   EHFQQTSEAKYDCLLFDIDDTLYPLSSGLAMEVKKNIQEYMVQKLGIEEDKVQELCLSLY 64

Query: 65  KIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTNGDM 124
           KIYGTTMAGL+A+GY+FDYDDFH FVHGRLPY  LKPDP+LRN++ SLPIRK +FTN D 
Sbjct: 65  KIYGTTMAGLKAVGYDFDYDDFHRFVHGRLPYSTLKPDPILRNIILSLPIRK-VFTNADK 124

Query: 125 AHANRALKRLGLEDCFEGILCFETLNP---DKGTVDEEAGSVIFDINQYMSNPNSDLDLP 184
           AHA + + RLGLE CFE I+ FETLNP    +  VD +    IFDI  YM+NP+S ++LP
Sbjct: 125 AHAAKIIARLGLEGCFEKIISFETLNPITKTESPVDTKTRE-IFDIISYMANPDSSIELP 184

Query: 185 KTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVD 244
           KT VVCKP E A++QVF++ANINPKKTLFFDDS+RN+QTGK VGLHTV VG S R +GVD
Sbjct: 185 KTSVVCKPSEGAFEQVFKMANINPKKTLFFDDSIRNIQTGKRVGLHTVWVGTSHREEGVD 244

Query: 245 HAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIET 278
            A E IHNI+E LP+LW+ ++       +R++VAIET
Sbjct: 245 IALEHIHNIREALPQLWDAVDDKAKEIRTRQKVAIET 279

BLAST of CSPI01G23220 vs. TAIR 10
Match: AT5G02230.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 342.0 bits (876), Expect = 4.4e-94
Identity = 172/282 (60.99%), Postives = 210/282 (74.47%), Query Frame = 0

Query: 1   MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGM-EENVPELC 60
           ME  + +   +  KY+CLLFDLDDTLYP +SG+A+E   NI++YM EKLG+ ++ + EL 
Sbjct: 1   MEFENRYGLATSQKYDCLLFDLDDTLYPLSSGIARECGNNIKDYMTEKLGIPKDKIVELS 60

Query: 61  ISLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFT 120
             LYK YGTTMAGLRAIGY FDYD++HSFVHGRLPYD +KPD +LR+LL SLP+RK IFT
Sbjct: 61  DLLYKNYGTTMAGLRAIGYEFDYDEYHSFVHGRLPYDNIKPDLVLRSLLLSLPLRKVIFT 120

Query: 121 NGDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEAGSVIFDINQYMSNPNSDLDL 180
           N D  HA +ALK+LGLEDCFEGI+CFETLN    T      S IFDI  + +       L
Sbjct: 121 NADRVHAAKALKKLGLEDCFEGIICFETLNL-MHTNAASNNSEIFDIVGHFNRSEPVGSL 180

Query: 181 PKTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGV 240
           PKTPVVCKP E A ++  EIANI+P +TLFF+DSVRN+Q GK VGL+TVLVG S ++KG 
Sbjct: 181 PKTPVVCKPSESAIEKALEIANIDPNRTLFFEDSVRNVQAGKRVGLYTVLVGKSTKVKGA 240

Query: 241 DHAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 282
           D+A E+IHN+KE +PELWE   K   V YS K VA+ETSVRA
Sbjct: 241 DYALENIHNMKEAIPELWESDRKSSDVGYSGK-VAVETSVRA 280

BLAST of CSPI01G23220 vs. TAIR 10
Match: AT5G02230.2 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 342.0 bits (876), Expect = 4.4e-94
Identity = 172/282 (60.99%), Postives = 210/282 (74.47%), Query Frame = 0

Query: 1   MENGDEFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGM-EENVPELC 60
           ME  + +   +  KY+CLLFDLDDTLYP +SG+A+E   NI++YM EKLG+ ++ + EL 
Sbjct: 1   MEFENRYGLATSQKYDCLLFDLDDTLYPLSSGIARECGNNIKDYMTEKLGIPKDKIVELS 60

Query: 61  ISLYKIYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFT 120
             LYK YGTTMAGLRAIGY FDYD++HSFVHGRLPYD +KPD +LR+LL SLP+RK IFT
Sbjct: 61  DLLYKNYGTTMAGLRAIGYEFDYDEYHSFVHGRLPYDNIKPDLVLRSLLLSLPLRKVIFT 120

Query: 121 NGDMAHANRALKRLGLEDCFEGILCFETLNPDKGTVDEEAGSVIFDINQYMSNPNSDLDL 180
           N D  HA +ALK+LGLEDCFEGI+CFETLN    T      S IFDI  + +       L
Sbjct: 121 NADRVHAAKALKKLGLEDCFEGIICFETLNL-MHTNAASNNSEIFDIVGHFNRSEPVGSL 180

Query: 181 PKTPVVCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGV 240
           PKTPVVCKP E A ++  EIANI+P +TLFF+DSVRN+Q GK VGL+TVLVG S ++KG 
Sbjct: 181 PKTPVVCKPSESAIEKALEIANIDPNRTLFFEDSVRNVQAGKRVGLYTVLVGKSTKVKGA 240

Query: 241 DHAFESIHNIKEGLPELWEDMEKLKSVTYSRKEVAIETSVRA 282
           D+A E+IHN+KE +PELWE   K   V YS K VA+ETSVRA
Sbjct: 241 DYALENIHNMKEAIPELWESDRKSSDVGYSGK-VAVETSVRA 280

BLAST of CSPI01G23220 vs. TAIR 10
Match: AT5G59490.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 310.5 bits (794), Expect = 1.4e-84
Identity = 155/281 (55.16%), Postives = 203/281 (72.24%), Query Frame = 0

Query: 6   EFNQISDAKYECLLFDLDDTLYPFNSGLAKEITKNIQEYMIEKLGMEEN-VPELCISLYK 65
           EF   S  +YECLLFDLDDTLYP +SGL+   + NI EYM+EKLG++E+ V EL   LYK
Sbjct: 2   EFVNSSPPRYECLLFDLDDTLYPLSSGLSDACSNNIIEYMVEKLGIDEDGVVELNQILYK 61

Query: 66  IYGTTMAGLRAIGYNFDYDDFHSFVHGRLPYDMLKPDPLLRNLLHSLPIRKFIFTNGDMA 125
            YGT+MAGL+A+GY FD D++H +VHGRLPY+ LKPDP+LR+LL  LP+RK +F+NGD  
Sbjct: 62  KYGTSMAGLKAVGYEFDNDEYHRYVHGRLPYENLKPDPVLRSLLLGLPLRKLVFSNGDEV 121

Query: 126 HANRALKRLGLEDCFEGILCFETLNPDKGTVDEEAGSVIFDINQYMSNPNSDLDLPKTPV 185
           H  +AL RLG+EDCFE I+ FETLNPD   ++E   S +               LP+ PV
Sbjct: 122 HVMKALTRLGIEDCFERIISFETLNPD---INEAELSCVTG------------HLPENPV 181

Query: 186 VCKPFEEAYKQVFEIANINPKKTLFFDDSVRNLQTGKLVGLHTVLVGNSQRIKGVDHAFE 245
           +CKP E A+++ F+IA +NP KTLFFDDS RN+QTGK VGLHTVLVG S++I G D+A E
Sbjct: 182 ICKPTEIAFEKAFDIAQLNPHKTLFFDDSTRNIQTGKAVGLHTVLVGKSEKIDGSDYALE 241

Query: 246 SIHNIKEGLPELWEDM----EKLKSVTYSRKEVAIETSVRA 282
           SIHN+KE  PELW +     ++ + + Y+  +++IETSV+A
Sbjct: 242 SIHNMKEAFPELWSESIINNKETERIDYA-SQISIETSVQA 266

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q098934.7e-1629.48Uncharacterized protein C24B11.05 OS=Schizosaccharomyces pombe (strain 972 / ATC... [more]
P400255.1e-1032.14Phosphate metabolism protein 8 OS=Saccharomyces cerevisiae (strain ATCC 204508 /... [more]
P530786.6e-1026.15Suppressor of disruption of TFIIS OS=Saccharomyces cerevisiae (strain ATCC 20450... [more]
Match NameE-valueIdentityDescription
A0A0A0LVD28.6e-16299.64Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G523620 PE=4 SV=1[more]
A0A1S4E1J36.3e-15797.16uncharacterized protein C24B11.05-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A5D3BED81.2e-15597.14Haloacid dehalogenase-like hydrolase superfamily protein OS=Cucumis melo var. ma... [more]
A0A5A7VPY61.2e-15597.14Haloacid dehalogenase-like hydrolase superfamily protein OS=Cucumis melo var. ma... [more]
A0A1S4E2932.8e-14496.95uncharacterized protein C24B11.05-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC... [more]
Match NameE-valueIdentityDescription
XP_011658227.11.8e-16199.64uncharacterized protein C24B11.05 [Cucumis sativus] >XP_011658231.1 uncharacteri... [more]
XP_016902092.11.3e-15697.16PREDICTED: uncharacterized protein C24B11.05-like isoform X1 [Cucumis melo] >XP_... [more]
KAA0067755.12.5e-15597.14Haloacid dehalogenase-like hydrolase superfamily protein [Cucumis melo var. maku... [more]
TYJ97389.12.5e-15597.14Haloacid dehalogenase-like hydrolase superfamily protein [Cucumis melo var. maku... [more]
XP_038893602.13.9e-15394.66uncharacterized protein LOC120082486 [Benincasa hispida] >XP_038893603.1 unchara... [more]
Match NameE-valueIdentityDescription
AT5G59480.14.7e-10464.98Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
AT5G59480.28.9e-10364.98Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
AT5G02230.14.4e-9460.99Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
AT5G02230.24.4e-9460.99Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
AT5G59490.11.4e-8455.16Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 34..54
NoneNo IPR availableSFLDSFLDS00003Haloacid_Dehalogenasecoord: 12..263
e-value: 0.0
score: 229.2
NoneNo IPR availablePANTHERPTHR12725HALOACID DEHALOGENASE-LIKE HYDROLASEcoord: 1..281
NoneNo IPR availablePANTHERPTHR12725:SF72HALOACID DEHALOGENASE-LIKE HYDROLASEcoord: 1..281
IPR023214HAD superfamilyGENE3D3.40.50.1000coord: 78..257
e-value: 1.7E-42
score: 146.7
IPR010237Pyrimidine 5-nucleotidaseTIGRFAMTIGR01993TIGR01993coord: 16..230
e-value: 2.4E-66
score: 221.0
IPR010237Pyrimidine 5-nucleotidaseSFLDSFLDG01132C1.5.3:_5'-Nucleotidase_Likecoord: 12..263
e-value: 0.0
score: 229.2
IPR010237Pyrimidine 5-nucleotidaseCDDcd02604HAD_5NTcoord: 17..232
e-value: 2.33293E-79
score: 235.991
IPR006439HAD hydrolase, subfamily IATIGRFAMTIGR01509TIGR01509coord: 183..230
e-value: 3.8E-11
score: 41.4
IPR041492Haloacid dehalogenase-like hydrolasePFAMPF13419HAD_2coord: 18..152
e-value: 3.6E-5
score: 23.9
IPR036412HAD-like superfamilySUPERFAMILY56784HAD-likecoord: 17..234

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G23220.1CSPI01G23220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity