CSPI01G21770 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G21770
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionEndoglucanase
LocationChr1: 17346180 .. 17348084 (+)
RNA-Seq ExpressionCSPI01G21770
SyntenyCSPI01G21770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGCAACCAGAGAGATCTGTCCACACAGAACATGAAGCAGATCGTTTTCTTAGCACGACACGACTTAACTTCAATGCCTTTTCTGAATCTTCTATTGAATATAATTCAATTCCTTCACCATACTCCAAGTCCTTTGACTTCAAGATCGTTATATCAAATCAAAGACGTTTCAAATGGTGTTCTTACATTTCAGCTTTGCTGCTACTGCTAATCATGGCACTTACACTTCTCCTTCAGTTTTTGCCTCACAAACATAATCTTCACGAAGCATCAAACAATTACACAGTTGCAGTAAATCAAGCACTAAAGTTTTTTGATGCTCAAAAATGTAGAAATCCTCCTTAACTAAAAATGTTGTGATGAACTTTTCATGCCCGACTGATAAAAAGCTTTTCTCAGCTGGTAGGTACCCGAAAAGTAGTCCAGTCAAGTTTCGGGGAGATTCAGGCTTGGAAGATGGGGTTTCAAGCAATAAACCAGATGGTCTCATTGGTGGTTTCTATGATTCTGGAAACAACATTAAGTTCACTTTCCCTACAGCTTATACCATTACTCTTTTAAGCTGGAGTGTGATTGAGTATCATCCGAAGTATGCAGACATGAATGAGCTTGATCATGTAAAGGACATCATCAGATGGGGAACTGAATATTTGCTCAAAATTTTTGTAGCCCCAAATGCAACTTCGGATCAAACCATAATATATTCTCAGGTAAGTCATATGAGCTCCTGATCAAGATACTTTTAGTGCAATTACCTCAACATTGAATTCTCCATGTCATGTTTATTACGTAACAGTCAACACTATCTCCAATGTGTGATCCTAGGTTGGCAGTTCCAGTAATGATAGTAATGCTCAAACCAATGACAACTGCTGGCAAAGACCAGAAAACATGATGTACCCCAGACCTATTTCAACATGTGATGCCCGGGCTTCGGATCTTGCTGGAGAAATCGTTGCAGCATTATCAGCTTCATCATTAGTATTCAGAGAAGATACCAACTATTCAAGAGAATTAGCAAAAGCTGCAGAGAAATTGTTTCAGCAAGTGACTAGATTAGACCCAATTGAGCAAGGAACTTACAGCTCAGTTGATTCTTGTGGAGGAGAAGCAAGAAAGTTCTACAACTCATCAAGTTACACGGATGAATTAATATGGGCAGGAACTTGGCTGTTCTTTGCAACTGGAAACACTTCATATCTTTCATATGCCACTGATGCTGTCCGATTTCAGTTAGCACAGAGTGAGGAAGCAAGTATTGGCAGAGGAATTTTCAATTGGAACAATAAGTTCAGTGCAACCGCGGTAACTCCAAATGTCTTATTTGAATTTCTGATAAAATGTAAAAGATCGAAGACAGATTTGACAAATGGCTTACATTACCACATTTTCCATAGGTATTATTGACACGCCTTCTCTACTTTCATGATACTGGCTATCCATATGAATATGCCTTAGGAGTATCATCAAATATGACAGAAATCCTCATGTGTTCTTATCTCATTGATCAACACTACGACAGGACACCTGGTAAACCATCCGTTCAGATCCCATGCATCAAAAACTTACTATCCCCACTGATTATTTTGTCAACAACAAGAATATGTCTAAGTTTGTTTCCAGTAATTGGGTAATCTTCCAATTGTTAATGTAGGTGGATTGATCCTCCTAAGGCCTGATGATGGAGCACCACTCCAATTTGCTGCAACAGCATCATTTCTCAGTAAATTGTACAGTGATTACCTTGATCTTTTGGGAGCATCTTACATGAGTTGCATTTTTGCCAATCCTGGCTTTTCTTTGGAAAAGTTGCGGAGCTTCTCCAACTCTCAGGCAAGTGCTGTATTAAGAATCATTTCTTTGTCTTCCATTTCAAAAACAGAAAAACGTAGAGCATTATAA

mRNA sequence

ATGATGCAACCAGAGAGATCTGTCCACACAGAACATGAAGCAGATCGTTTTCTTAGCACGACACGACTTAACTTCAATGCCTTTTCTGAATCTTCTATTGAATATAATTCAATTCCTTCACCATACTCCAAGTCCTTTGACTTCAAGATCGTTATATCAAATCAAAGACGTTTCAAATGGTGTTCTTACATTTCAGCTTTGCTGCTACTGCTAATCATGGCACTTACACTTCTCCTTCAGTTTTTGCCTCACAAACATAATCTTCACGAAGCATCAAACAATTACACAGTTGCAAAATCCTCCTTAACTAAAAATGTTGTGATGAACTTTTCATGCCCGACTGATAAAAAGCTTTTCTCAGCTGGTAGGTACCCGAAAAGTAGTCCAGTCAAGTTTCGGGGAGATTCAGGCTTGGAAGATGGGGTTTCAAGCAATAAACCAGATGGTCTCATTGGTGGTTTCTATGATTCTGGAAACAACATTAAGTTCACTTTCCCTACAGCTTATACCATTACTCTTTTAAGCTGGAGTGTGATTGAGTATCATCCGAAGTATGCAGACATGAATGAGCTTGATCATGTAAAGGACATCATCAGATGGGGAACTGAATATTTGCTCAAAATTTTTGTAGCCCCAAATGCAACTTCGGATCAAACCATAATATATTCTCAGGTTGGCAGTTCCAGTAATGATAGTAATGCTCAAACCAATGACAACTGCTGGCAAAGACCAGAAAACATGATGTACCCCAGACCTATTTCAACATGTGATGCCCGGGCTTCGGATCTTGCTGGAGAAATCGTTGCAGCATTATCAGCTTCATCATTAGTATTCAGAGAAGATACCAACTATTCAAGAGAATTAGCAAAAGCTGCAGAGAAATTGTTTCAGCAAGTGACTAGATTAGACCCAATTGAGCAAGGAACTTACAGCTCAGTTGATTCTTGTGGAGGAGAAGCAAGAAAGTTCTACAACTCATCAAGTTACACGGATGAATTAATATGGGCAGGAACTTGGCTGTTCTTTGCAACTGGAAACACTTCATATCTTTCATATGCCACTGATGCTGTCCGATTTCAGTTAGCACAGAGTGAGGAAGCAAGTATTGGCAGAGGAATTTTCAATTGGAACAATAAGTTCAGTGCAACCGCGGTATTATTGACACGCCTTCTCTACTTTCATGATACTGGCTATCCATATGAATATGCCTTAGGAGTATCATCAAATATGACAGAAATCCTCATGTGTTCTTATCTCATTGATCAACACTACGACAGGACACCTGGTGGATTGATCCTCCTAAGGCCTGATGATGGAGCACCACTCCAATTTGCTGCAACAGCATCATTTCTCAGTAAATTGTACAGTGATTACCTTGATCTTTTGGGAGCATCTTACATGAGTTGCATTTTTGCCAATCCTGGCTTTTCTTTGGAAAAGTTGCGGAGCTTCTCCAACTCTCAGGCAAGTGCTGTATTAAGAATCATTTCTTTGTCTTCCATTTCAAAAACAGAAAAACGTAGAGCATTATAA

Coding sequence (CDS)

ATGATGCAACCAGAGAGATCTGTCCACACAGAACATGAAGCAGATCGTTTTCTTAGCACGACACGACTTAACTTCAATGCCTTTTCTGAATCTTCTATTGAATATAATTCAATTCCTTCACCATACTCCAAGTCCTTTGACTTCAAGATCGTTATATCAAATCAAAGACGTTTCAAATGGTGTTCTTACATTTCAGCTTTGCTGCTACTGCTAATCATGGCACTTACACTTCTCCTTCAGTTTTTGCCTCACAAACATAATCTTCACGAAGCATCAAACAATTACACAGTTGCAAAATCCTCCTTAACTAAAAATGTTGTGATGAACTTTTCATGCCCGACTGATAAAAAGCTTTTCTCAGCTGGTAGGTACCCGAAAAGTAGTCCAGTCAAGTTTCGGGGAGATTCAGGCTTGGAAGATGGGGTTTCAAGCAATAAACCAGATGGTCTCATTGGTGGTTTCTATGATTCTGGAAACAACATTAAGTTCACTTTCCCTACAGCTTATACCATTACTCTTTTAAGCTGGAGTGTGATTGAGTATCATCCGAAGTATGCAGACATGAATGAGCTTGATCATGTAAAGGACATCATCAGATGGGGAACTGAATATTTGCTCAAAATTTTTGTAGCCCCAAATGCAACTTCGGATCAAACCATAATATATTCTCAGGTTGGCAGTTCCAGTAATGATAGTAATGCTCAAACCAATGACAACTGCTGGCAAAGACCAGAAAACATGATGTACCCCAGACCTATTTCAACATGTGATGCCCGGGCTTCGGATCTTGCTGGAGAAATCGTTGCAGCATTATCAGCTTCATCATTAGTATTCAGAGAAGATACCAACTATTCAAGAGAATTAGCAAAAGCTGCAGAGAAATTGTTTCAGCAAGTGACTAGATTAGACCCAATTGAGCAAGGAACTTACAGCTCAGTTGATTCTTGTGGAGGAGAAGCAAGAAAGTTCTACAACTCATCAAGTTACACGGATGAATTAATATGGGCAGGAACTTGGCTGTTCTTTGCAACTGGAAACACTTCATATCTTTCATATGCCACTGATGCTGTCCGATTTCAGTTAGCACAGAGTGAGGAAGCAAGTATTGGCAGAGGAATTTTCAATTGGAACAATAAGTTCAGTGCAACCGCGGTATTATTGACACGCCTTCTCTACTTTCATGATACTGGCTATCCATATGAATATGCCTTAGGAGTATCATCAAATATGACAGAAATCCTCATGTGTTCTTATCTCATTGATCAACACTACGACAGGACACCTGGTGGATTGATCCTCCTAAGGCCTGATGATGGAGCACCACTCCAATTTGCTGCAACAGCATCATTTCTCAGTAAATTGTACAGTGATTACCTTGATCTTTTGGGAGCATCTTACATGAGTTGCATTTTTGCCAATCCTGGCTTTTCTTTGGAAAAGTTGCGGAGCTTCTCCAACTCTCAGGCAAGTGCTGTATTAAGAATCATTTCTTTGTCTTCCATTTCAAAAACAGAAAAACGTAGAGCATTATAA

Protein sequence

MMQPERSVHTEHEADRFLSTTRLNFNAFSESSIEYNSIPSPYSKSFDFKIVISNQRRFKWCSYISALLLLLIMALTLLLQFLPHKHNLHEASNNYTVAKSSLTKNVVMNFSCPTDKKLFSAGRYPKSSPVKFRGDSGLEDGVSSNKPDGLIGGFYDSGNNIKFTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDIIRWGTEYLLKIFVAPNATSDQTIIYSQVGSSSNDSNAQTNDNCWQRPENMMYPRPISTCDARASDLAGEIVAALSASSLVFREDTNYSRELAKAAEKLFQQVTRLDPIEQGTYSSVDSCGGEARKFYNSSSYTDELIWAGTWLFFATGNTSYLSYATDAVRFQLAQSEEASIGRGIFNWNNKFSATAVLLTRLLYFHDTGYPYEYALGVSSNMTEILMCSYLIDQHYDRTPGGLILLRPDDGAPLQFAATASFLSKLYSDYLDLLGASYMSCIFANPGFSLEKLRSFSNSQASAVLRIISLSSISKTEKRRAL*
Homology
BLAST of CSPI01G21770 vs. ExPASy Swiss-Prot
Match: Q38890 (Endoglucanase 25 OS=Arabidopsis thaliana OX=3702 GN=KOR PE=1 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 7.6e-81
Identity = 187/460 (40.65%), Postives = 265/460 (57.61%), Query Frame = 0

Query: 44  KSFDFKIVISNQRRFKWCSYISALLLLLIMALTLLLQFLPHKHNLHEASNNYTVAKSSLT 103
           K  D   +I +++ F W         LL   +TL+++ +P  H      +NYT+A   L 
Sbjct: 58  KYVDLGCIIVSRKIFVWTVGTLVAAALLAGFITLIVKTVPRHHPKTPPPDNYTIA---LH 117

Query: 104 KNVVMNFSCPTDKKLFSA---GRYPKSSPVKFRGDSGLEDGVSSNKP--DGLIGGFYDSG 163
           K +          K F+A   G+ PK + V +RG+SGL+DG          L+GG+YD+G
Sbjct: 118 KAL----------KFFNAQKSGKLPKHNNVSWRGNSGLQDGKGETGSFYKDLVGGYYDAG 177

Query: 164 NNIKFTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDIIRWGTEYLLKIFVAPNATSDQ 223
           + IKF FP AY +T+LSWSVIEY  KY    EL HVK++I+WGT+Y LK F   N+T+D 
Sbjct: 178 DAIKFNFPMAYAMTMLSWSVIEYSAKYEAAGELTHVKELIKWGTDYFLKTF---NSTADS 237

Query: 224 -TIIYSQVGS-SSNDSNAQTNDN-CWQRPENMMYPRPISTCDARASDLAGEIVAALSASS 283
              + SQVGS +++D N   ND+ CW RPE+M Y RP++TC+   SDLA E+ AAL+++S
Sbjct: 238 IDDLVSQVGSGNTDDGNTDPNDHYCWMRPEDMDYKRPVTTCNGGCSDLAAEMAAALASAS 297

Query: 284 LVFREDTNYSRELAKAAEKLFQQVTRLDPIEQGTYSSVDSCGGEARKFYNSSSYTDELIW 343
           +VF+++  YS++L   A+ ++Q         +G YS+  +   E+ KFYNSS Y DE IW
Sbjct: 298 IVFKDNKEYSKKLVHGAKVVYQ----FGRTRRGRYSAGTA---ESSKFYNSSMYWDEFIW 357

Query: 344 AGTWLFFATGNTSYLSYATDAVRFQLAQSEEASIGRGIFNWNNKFSATAVLLTRLLYFHD 403
            G W+++ATGN +YL+  T     + A +       G+F+W+NK +   +LL+RL  F  
Sbjct: 358 GGAWMYYATGNVTYLNLITQPTMAKHAGAFWGGPYYGVFSWDNKLAGAQLLLSRLRLFLS 417

Query: 404 TGYPYEYALGVSSNMTEILMCSYL-IDQHYDRTPGGLILLRPDDGAPLQFAATASFLSKL 463
            GYPYE  L    N T I+MCSYL I   ++RT GGLI L      PLQ++  A+FL+ L
Sbjct: 418 PGYPYEEILRTFHNQTSIVMCSYLPIFNKFNRTNGGLIELNHGAPQPLQYSVNAAFLATL 477

Query: 464 YSDYLDLLGASYMSCIFANPGF-SLEKLRSFSNSQASAVL 494
           YSDYLD        C    P F S   LR F+ SQ   +L
Sbjct: 478 YSDYLDAADTPGWYC---GPNFYSTSVLRDFARSQIDYIL 491

BLAST of CSPI01G21770 vs. ExPASy Swiss-Prot
Match: P0C1U4 (Endoglucanase 9 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU1 PE=2 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 7.3e-76
Identity = 171/455 (37.58%), Postives = 261/455 (57.36%), Query Frame = 0

Query: 44  KSFDFKIVISNQRRFKWCSYISALLLLLIMA--LTLLLQFLPHKHNLHEASNNYTVAKSS 103
           K  D   ++ +++ F W   +  LL   + A  +  + + +P  H      +++TVA   
Sbjct: 61  KYVDLGCLVVSRKLFVWT--VGVLLAAAVFAGLVAGIAKAIPRHHRPPPPPDDFTVA--- 120

Query: 104 LTKNVVMNFSCPTDKKLFSAGRYPKSSPVKFRGDSGLEDGVSSNKPD-GLIGGFYDSGNN 163
             +  +M F+         +G+ PK++ V +RG+S ++DG+S       L+GG+YD+G+ 
Sbjct: 121 -LRKALMFFNAQ------KSGKLPKNNNVHWRGNSCMKDGLSDPAVGRSLVGGYYDAGDA 180

Query: 164 IKFTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDIIRWGTEYLLKIFVAPNATSDQTI 223
           +KF FP A+++TLLSWSVIEY  KY  + EL H++D I+WG +Y LK F +   T D+ +
Sbjct: 181 VKFNFPAAFSMTLLSWSVIEYSAKYEAVGELGHIRDTIKWGADYFLKTFNSTADTIDRVV 240

Query: 224 IYSQVGSSSNDSNAQTNDNCWQRPENMMYPRPISTCDARASDLAGEIVAALSASSLVFRE 283
           +    G++S  S    +  CW RPE++ YPRP+  C A  SDLA E+ A+L+A+S+VF++
Sbjct: 241 MQVGSGATSPGSTQPNDHYCWMRPEDIDYPRPVVECHA-CSDLAAEMAASLAAASIVFKD 300

Query: 284 DTNYSRELAKAAEKLFQQVTRLDPIEQGTYSSVDSCGGEARKFYNSSSYTDELIWAGTWL 343
           +  YS++L   A  LF+   +     +G YS   + G +A KFYNS+SY DE +W G+W+
Sbjct: 301 NKAYSQKLVHGATTLFKFARQ----NRGRYS---AGGSDAAKFYNSTSYWDEFVWGGSWM 360

Query: 344 FFATGNTSYLSYATDAVRFQLAQSEEASIGRGIFNWNNKFSATAVLLTRLLYFHDTGYPY 403
           + ATGN+SYL  AT     + A +       G+F+W+NK +   VLL+RL  F   GYPY
Sbjct: 361 YLATGNSSYLQLATHPKLAKHAGAYWGGPDYGVFSWDNKLTGAQVLLSRLRLFLSPGYPY 420

Query: 404 EYALGVSSNMTEILMCSYL-IDQHYDRTPGGLILLRPDDGAPLQFAATASFLSKLYSDYL 463
           E  L    N T I+MCSYL I + ++RT GGLI L      PLQ+   A+FL+ LY DYL
Sbjct: 421 EEILRTFHNQTSIIMCSYLPIFKSFNRTKGGLIQLNHGRPQPLQYVVNAAFLASLYGDYL 480

Query: 464 DLLGASYMSCIFANPGF-SLEKLRSFSNSQASAVL 494
           +        C    P F  +E LR+F+ +Q   +L
Sbjct: 481 EAADTPGWYC---GPHFYPIETLRNFARTQIEYIL 492

BLAST of CSPI01G21770 vs. ExPASy Swiss-Prot
Match: Q84R49 (Endoglucanase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU2 PE=2 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 4.8e-75
Identity = 178/455 (39.12%), Postives = 260/455 (57.14%), Query Frame = 0

Query: 44  KSFDFKIVISNQRRFKWCSYISALLLLLIMALTLLLQFLPHKHNLHEASNNYTVAKSSLT 103
           K  D   ++ +++ F W       + L I  + ++++ +PHK       + YT A     
Sbjct: 59  KYVDLGCMVLDRKIFMWTVGTILGVGLFIGFVMMIVKLVPHKRPPPPPPDQYTQA----L 118

Query: 104 KNVVMNFSCPTDKKLFSAGRYPKSSPVKFRGDSGLEDGVS-SNKPDGLIGGFYDSGNNIK 163
              +M F+         +G  PK + V +RG+S ++DG+S S     L+GGFYD+G+ IK
Sbjct: 119 HKALMFFNAQ------RSGPLPKHNGVSWRGNSCMKDGLSDSTVRKSLVGGFYDAGDAIK 178

Query: 164 FTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDIIRWGTEYLLKIFVAPNATSDQTIIY 223
           F +P A+++T+LSWSVIEY  KY  + ELDHVK++I+WGT+YLLK F +   T D+ +  
Sbjct: 179 FNYPMAWSMTMLSWSVIEYKAKYEAIGELDHVKELIKWGTDYLLKTFNSSADTIDRIVAQ 238

Query: 224 SQVGSSSNDSNAQTNDN-CWQRPENMMYPRPISTCDARASDLAGEIVAALSASSLVFRED 283
             VG +S    AQ ND+ CW RPE++ YPRP++ C +  SDLA E+ AAL+A+S+VF++ 
Sbjct: 239 VGVGDTSK-GGAQPNDHYCWMRPEDIDYPRPVTECHS-CSDLASEMAAALAAASIVFKDS 298

Query: 284 TNYSRELAKAAEKLFQQVTRLDPIEQGTYSSVDSCGGEARKFYNSSSYTDELIWAGTWLF 343
             YS +L + A+ L+    +   +++G YS     G +   FYNS+SY DE +W G W++
Sbjct: 299 KTYSDKLVRGAKALY----KFGRLQRGRYS---PNGSDQAIFYNSTSYWDEFVWGGAWMY 358

Query: 344 FATGNTSYLSYATDAVRFQLAQSEEA-SIGRGIFNWNNKFSATAVLLTRLLYFHDTGYPY 403
           FATGN +YLS AT     + A +    S   G+F W++K     VLL+RL  F   GYPY
Sbjct: 359 FATGNNTYLSVATAPGMAKHAGAYWLDSPNYGVFTWDDKLPGAQVLLSRLRLFLSPGYPY 418

Query: 404 EYALGVSSNMTEILMCSYL-IDQHYDRTPGGLILLRPDDGAPLQFAATASFLSKLYSDYL 463
           E  L    N T+ +MCSYL +   ++ T GG+I L      PLQ+   A+FL+ LYSDYL
Sbjct: 419 EEILRTFHNQTDNVMCSYLPMYNSFNFTKGGMIQLNHGRPQPLQYVVNAAFLASLYSDYL 478

Query: 464 DLLGASYMSCIFANPGF-SLEKLRSFSNSQASAVL 494
           D        C    P F + E LR F+ SQ   VL
Sbjct: 479 DAADTPGWYC---GPTFYTTEVLRKFARSQLDYVL 491

BLAST of CSPI01G21770 vs. ExPASy Swiss-Prot
Match: Q9STW8 (Endoglucanase 21 OS=Arabidopsis thaliana OX=3702 GN=KOR3 PE=2 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 8.1e-75
Identity = 179/461 (38.83%), Postives = 258/461 (55.97%), Query Frame = 0

Query: 44  KSFDFKIVISNQRRFKWCSYISALLLLLIMALTLLLQFLPHKHNLHEASNNYTVAKSSLT 103
           K  D   ++ +++ F W      +  LL   +TL+++ LPH H+     +NYT+A     
Sbjct: 58  KYVDLGCILVSRKIFLWTLGTIVVTALLSGFITLIVKTLPHHHHKEPPPDNYTIA----- 117

Query: 104 KNVVMNFSCPTDKKLFSA---GRYPKS-SPVKFRGDSGLEDGVSSNKP--DGLIGGFYDS 163
                     T  K F+A   G+ PK+   V +R DS L+DG          L+GG+YD+
Sbjct: 118 --------LRTALKFFNAQQSGKLPKNIYNVSWRHDSCLQDGKGDPGQCYKDLVGGYYDA 177

Query: 164 GNNIKFTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDIIRWGTEYLLKIFVAPNATSD 223
           G++IKF FP +Y +T+LSWSVIEY  KY    EL+HVK++I+WGT+Y LK F   N+++D
Sbjct: 178 GDSIKFNFPMSYAMTMLSWSVIEYSAKYQAAGELEHVKELIKWGTDYFLKTF---NSSAD 237

Query: 224 QT-IIYSQVGS--SSNDSNAQTNDNCWQRPENMMYPRPISTCDARASDLAGEIVAALSAS 283
              ++  QVGS  S   S    +  CW RPE++ Y R +S C +  SDLA E+ AAL+++
Sbjct: 238 NIYVMVEQVGSGVSGRGSELHNDHYCWMRPEDIHYKRTVSQCYSSCSDLAAEMAAALASA 297

Query: 284 SLVFREDTNYSRELAKAAEKLFQQVTRLDPIEQGTYSSVDSCGGEARKFYNSSSYTDELI 343
           S+VF+++  YS+ L   A+ L++  T      +  YS     G E+ KFYNSS + DEL+
Sbjct: 298 SIVFKDNRLYSKNLVHGAKTLYRFAT----TSRNRYS---QNGKESSKFYNSSMFEDELL 357

Query: 344 WAGTWLFFATGNTSYLSYATDAVRFQLAQSEEASIGRGIFNWNNKFSATAVLLTRLLYFH 403
           W G WL++ATGN +YL   T     + A +   S   G+F+W+NK     +LLTR+  F 
Sbjct: 358 WGGAWLYYATGNVTYLERVTSHHMAEKAGAFGNSPYYGVFSWDNKLPGAQLLLTRMRLFL 417

Query: 404 DTGYPYEYALGVSSNMTEILMCSYL-IDQHYDRTPGGLILLRPDDGAPLQFAATASFLSK 463
             GYPYE  L    N T  +MCSYL   + ++RT GGLI L      PLQ+ A A+FL+ 
Sbjct: 418 SPGYPYEDMLSEFHNQTGRVMCSYLPYYKKFNRTNGGLIQLNHGAPQPLQYVANAAFLAA 477

Query: 464 LYSDYLDLLGASYMSCIFANPGF-SLEKLRSFSNSQASAVL 494
           L+SDYL+        C    P F + E LR+FS SQ   +L
Sbjct: 478 LFSDYLEAADTPGWYC---GPNFYTTEFLRNFSRSQIDYIL 492

BLAST of CSPI01G21770 vs. ExPASy Swiss-Prot
Match: Q7XUK4 (Endoglucanase 12 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU3 PE=2 SV=2)

HSP 1 Score: 272.3 bits (695), Expect = 1.1e-71
Identity = 164/456 (35.96%), Postives = 258/456 (56.58%), Query Frame = 0

Query: 44  KSFDFKIVISNQRRFKWCSYISALLLLLIMALTLLLQFLPHKHNLHEASNNYTVAKSSLT 103
           K  D   V+  ++   W  +      +LI    ++ + +P K       + YT A     
Sbjct: 60  KYVDLGCVVVKRKLLWWVLWTLLAAFILIGLPVIIAKSIPKKKPHAPPPDQYTDA----L 119

Query: 104 KNVVMNFSCPTDKKLFSAGRYPKSSPVKFRGDSGLEDGVS-SNKPDGLIGGFYDSGNNIK 163
              ++ F+         +GR PK++ +K+RG+SGL DG   ++   GL+GG+YD+G+NIK
Sbjct: 120 HKALLFFNAQ------KSGRLPKNNGIKWRGNSGLSDGSDLTDVKGGLVGGYYDAGDNIK 179

Query: 164 FTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDIIRWGTEYLLKIFVAPNATSDQTIIY 223
           F FP A+++T+LSWSVIEY  KY  + E DHV+++I+WGT+YLL  F +  +T D+  +Y
Sbjct: 180 FHFPLAFSMTMLSWSVIEYSAKYKAVGEYDHVRELIKWGTDYLLLTFNSSASTIDK--VY 239

Query: 224 SQVGSSSNDSNAQTNDNCWQRPENMMYPRPISTCDARASDLAGEIVAALSASSLVFREDT 283
           SQVG +  +     +  CW RPE+M YPRP+ T  + A DL GE+ AAL+A+S+VFR++ 
Sbjct: 240 SQVGIAKINGTQPDDHYCWNRPEDMAYPRPVQTAGS-APDLGGEMAAALAAASIVFRDNA 299

Query: 284 NYSREL---AKAAEKLFQQVTRLDPIEQGTYSSVDSCGGEARKFYNSSSYTDELIWAGTW 343
            YS++L   A A  K  +   R  P  +G              +YNS+SY DE +W+  W
Sbjct: 300 AYSKKLVNGAAAVYKFARSSGRRTPYSRGNQ--------YIEYYYNSTSYWDEYMWSAAW 359

Query: 344 LFFATGNTSYLSYATDAVRFQLAQSEEASIGRGIFNWNNKFSATAVLLTRLLYFHDTGYP 403
           +++ATGN +Y+++ATD    + A++  + +   +F+W+NK     +LL+RL  F + GYP
Sbjct: 360 MYYATGNNTYITFATDPRLPKNAKAFYSILDFSVFSWDNKLPGAELLLSRLRMFLNPGYP 419

Query: 404 YEYALGVSSNMTEILMCSYLID-QHYDRTPGGLILLRPDDGAPLQFAATASFLSKLYSDY 463
           YE +L    N T + MC+Y      ++ T GGL       G PLQ+    SFL+ LY+DY
Sbjct: 420 YEESLIGYHNTTSMNMCTYFPRFGAFNFTKGGLAQFNHGKGQPLQYTVANSFLAALYADY 479

Query: 464 LDLLGASYMSCIFANPGF-SLEKLRSFSNSQASAVL 494
           ++ +      C    P F +++ LRSF+ SQ + +L
Sbjct: 480 MESVNVPGWYC---GPYFMTVDDLRSFARSQVNYIL 491

BLAST of CSPI01G21770 vs. ExPASy TrEMBL
Match: A0A5A7UU46 (Endoglucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G002590 PE=3 SV=1)

HSP 1 Score: 847.8 bits (2189), Expect = 2.3e-242
Identity = 431/476 (90.55%), Postives = 444/476 (93.28%), Query Frame = 0

Query: 21  TRLNFNAFSESSIEYNSIPSPYSKSFDFKIVISNQRRFKWCSYISALLLLLIMALTLLLQ 80
           TR NFN FS+SS EYNSIPSPYSKSFDFKIVISNQRRFK CSYISALLLLLI+ALTLLLQ
Sbjct: 2   TRHNFNVFSKSSTEYNSIPSPYSKSFDFKIVISNQRRFKCCSYISALLLLLIIALTLLLQ 61

Query: 81  FLPHKHNLHEASNNYTVAKSSLTKNVVMNFSCPTDKKLFSAGRYPKSSPVKFRGDSGLED 140
           FLPHKHNLHEASNNYTVA                   LFSAGRYPKSSPVKFRGDSGL+D
Sbjct: 62  FLPHKHNLHEASNNYTVA-------------------LFSAGRYPKSSPVKFRGDSGLKD 121

Query: 141 GVSSNKPDGLIGGFYDSGNNIKFTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDIIRW 200
           GVSSNKPDGLIGGFYDSGNN+KFTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDIIRW
Sbjct: 122 GVSSNKPDGLIGGFYDSGNNMKFTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDIIRW 181

Query: 201 GTEYLLKIFVAPNATSDQTIIYSQVGSSSNDSNAQTNDNCWQRPENMMYPRPISTCDARA 260
           GTEYLLK+FVAPNATSDQTIIYSQVGSSSN+S AQTNDNCWQRPE+MMYPRP+STCDARA
Sbjct: 182 GTEYLLKVFVAPNATSDQTIIYSQVGSSSNESKAQTNDNCWQRPEDMMYPRPVSTCDARA 241

Query: 261 SDLAGEIVAALSASSLVFREDTNYSRELAKAAEKLFQQVTRLDPIEQGTYSSVDSCGGEA 320
           SDLAGEIVAALSASSLVFREDTNYS ELAKAAEKLFQQVT+LDPIEQGTYSSVDSCGGEA
Sbjct: 242 SDLAGEIVAALSASSLVFREDTNYSGELAKAAEKLFQQVTKLDPIEQGTYSSVDSCGGEA 301

Query: 321 RKFYNSSSYTDELIWAGTWLFFATGNTSYLSYATDAVRFQLAQSEEASIGRGIFNWNNKF 380
           RKFYNSSSYTDELIWAGTWLFFATGNTSYLSYATDAVRFQLAQSEEASIGRGIFNWNNKF
Sbjct: 302 RKFYNSSSYTDELIWAGTWLFFATGNTSYLSYATDAVRFQLAQSEEASIGRGIFNWNNKF 361

Query: 381 SATAVLLTRLLYFHDTGYPYEYALGVSSNMTEILMCSYLIDQHYDRTPGGLILLRPDDGA 440
           SATAVLLTRLLYFHDTGYPYEYALGVSSNMTEILMCSYLIDQH++RTP GLILL PDD A
Sbjct: 362 SATAVLLTRLLYFHDTGYPYEYALGVSSNMTEILMCSYLIDQHFNRTPSGLILLSPDDKA 421

Query: 441 PLQFAATASFLSKLYSDYLDLLGASYMSCIFANPGFSLEKLRSFSNSQASAVLRII 497
           PLQFAATASFLSKLYSDYLDLLGASYMSCIFANP FSLEKLRSFS SQASA+  I+
Sbjct: 422 PLQFAATASFLSKLYSDYLDLLGASYMSCIFANPRFSLEKLRSFSKSQASALNYIL 458

BLAST of CSPI01G21770 vs. ExPASy TrEMBL
Match: A0A6J1CED2 (Endoglucanase OS=Momordica charantia OX=3673 GN=LOC111010902 PE=3 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 4.0e-226
Identity = 395/492 (80.28%), Postives = 435/492 (88.41%), Query Frame = 0

Query: 1   MMQPERSVHTEHEADRFLSTTRLNFNAFSESSIEYNSIPSPYSKSFDFKIVISNQRRFKW 60
           MMQP R VH EHEA+R LS+TRL+ N    +SI Y+SIPSPYSKSFDFK+VISN+ RF+W
Sbjct: 1   MMQPVRPVHAEHEANRLLSSTRLDSNVSPRASIGYDSIPSPYSKSFDFKMVISNRTRFRW 60

Query: 61  CSYISALLLLLIMALTLLLQFLPHKHNLHEASNNYTVAKSSLTKNVVMNFSCPTDKKLFS 120
           CSYISALLLLLI+A++ LL FLPHKHN HEASNN+TVA +   K                
Sbjct: 61  CSYISALLLLLIIAVSFLLHFLPHKHNHHEASNNHTVAMNQALKFFDAQ----------K 120

Query: 121 AGRYPKSSPVKFRGDSGLEDGVSSNKPDGLIGGFYDSGNNIKFTFPTAYTITLLSWSVIE 180
           +GRYPK+SPVKFRGDSGLEDGV  NK DGL+GGFYDSGNNIKFTFPTAYTITLLSWSVIE
Sbjct: 121 SGRYPKNSPVKFRGDSGLEDGVVDNKLDGLVGGFYDSGNNIKFTFPTAYTITLLSWSVIE 180

Query: 181 YHPKYADMNELDHVKDIIRWGTEYLLKIFVAPNATSDQTIIYSQVGSSSNDSNAQTNDNC 240
           YHPKYADMNELDHV+DIIRWGT+YLLK+FVAPN TSDQ IIYSQVGS+SNDSN QTNDNC
Sbjct: 181 YHPKYADMNELDHVEDIIRWGTDYLLKVFVAPNGTSDQAIIYSQVGSASNDSNVQTNDNC 240

Query: 241 WQRPENMMYPRPISTCDARASDLAGEIVAALSASSLVFREDTNYSRELAKAAEKLFQQVT 300
           WQRPE+  YPRP+S CD RASDLAGEIVAALSA+SLVF+ED NYS ELAKAAEKLF++VT
Sbjct: 241 WQRPEDTRYPRPVSKCDTRASDLAGEIVAALSAASLVFKEDNNYSGELAKAAEKLFEEVT 300

Query: 301 RLDPIEQGTYSSVDSCGGEARKFYNSSSYTDELIWAGTWLFFATGNTSYLSYATDAVRFQ 360
           +LDP EQGTY+ VDSCGGEAR FYNSSSY DELIWAGTWLF+ATGNTSYL+YATDAVRFQ
Sbjct: 301 KLDPSEQGTYTLVDSCGGEARNFYNSSSYMDELIWAGTWLFYATGNTSYLAYATDAVRFQ 360

Query: 361 LAQSEEASIGRGIFNWNNKFSATAVLLTRLLYFHDTGYPYEYALGVSSNMTEILMCSYLI 420
           LAQS+E+SI RGIF+WNNKFSATAVLLTRLLYFHD  YPYE+ALG SSN T+ILMCSYLI
Sbjct: 361 LAQSKESSIDRGIFDWNNKFSATAVLLTRLLYFHDIVYPYEHALGASSNKTDILMCSYLI 420

Query: 421 DQHYDRTPGGLILLRPDDGAPLQFAATASFLSKLYSDYLDLLGASYMSCIFANPGFSLEK 480
           DQH++RTPGGLI+LRPD GAPLQFAATASFLSKLYSDYLDLLGASYMSCIFANPGFSLEK
Sbjct: 421 DQHFNRTPGGLIILRPDGGAPLQFAATASFLSKLYSDYLDLLGASYMSCIFANPGFSLEK 480

Query: 481 LRSFSNSQASAV 493
           LR+FS SQASA+
Sbjct: 481 LRTFSRSQASAI 482

BLAST of CSPI01G21770 vs. ExPASy TrEMBL
Match: A0A6J1ACP6 (Endoglucanase OS=Herrania umbratica OX=108875 GN=LOC110416795 PE=3 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 1.3e-152
Identity = 293/495 (59.19%), Postives = 361/495 (72.93%), Query Frame = 0

Query: 8   VHTEHEADRFL------STTRLNFNAFSESSIEYNSIPSPYSKSFDFKIVISNQRRFKWC 67
           VH   EA R L      ++  L+F    +SS  Y+S+PS YSKS+D+++VI+++  +K  
Sbjct: 19  VHAISEAGRLLPSASKWNSIELDFKVLPQSSTGYDSLPSAYSKSYDYELVITDKAHYKRF 78

Query: 68  SYISALLLLLIMALTLLLQFLPHKHNLHEASNNYTVAKSSLTKNVVMNFSCPTDKKLFSA 127
            YIS  +  LI+AL L+L FLP K++ HE+S N      SL  N  + F          +
Sbjct: 79  LYISLTVAFLILALGLVLHFLPRKNHHHESSKNL-----SLAVNQAITFF-----DAQKS 138

Query: 128 GRYPKSSPVKFRGDSGLEDGVSSNKPDGLIGGFYDSGNNIKFTFPTAYTITLLSWSVIEY 187
           G YP  SP+KFRG SGL DG + N    L+GGFYDSGNNIKFTFPTAYTITLLSWSVIEY
Sbjct: 139 GNYPSKSPIKFRGSSGLRDGNTRNTRADLVGGFYDSGNNIKFTFPTAYTITLLSWSVIEY 198

Query: 188 HPKYADMNELDHVKDIIRWGTEYLLKIFVAPNATSDQTIIYSQVGSSSNDS-NAQTND-N 247
           H KYAD+ EL+H+KDIIRWG++YLLK+FVAPNATS+ TI+YSQVGS+ ND+ N  +ND N
Sbjct: 199 HQKYADIGELEHIKDIIRWGSDYLLKVFVAPNATSEPTILYSQVGSAGNDTRNPGSNDIN 258

Query: 248 CWQRPENMMYPRPISTCDARASDLAGEIVAALSASSLVFREDTNYSRELAKAAEKLFQQV 307
           CWQRPE+M Y RP+S CD  ASDLAGEIVAALSA+S+VF+E+  +S+ L KAAEKL+   
Sbjct: 259 CWQRPEDMSYYRPVSVCDETASDLAGEIVAALSAASIVFKEENEHSQRLTKAAEKLYGIA 318

Query: 308 TRLDPIEQG-TYSSVDSCGGEARKFYNSSSYTDELIWAGTWLFFATGNTSYLSYATDAVR 367
            + D I +  TY+++D+CGGEARKFYNSS Y DEL+W GTWLFFATGN +YL YAT    
Sbjct: 319 EKKDKIHKAVTYTTIDACGGEARKFYNSSGYKDELVWGGTWLFFATGNYTYLDYAT--TN 378

Query: 368 FQLAQSEEASIGRGIFNWNNKFSATAVLLTRLLYFHDTGYPYEYALGVSSNMTEILMCSY 427
           F  A + E    +GIF WNNK +ATA LLTRL +FHD G+PYE ALG+SS MT+ LMCSY
Sbjct: 379 FAAASNNETIADKGIFYWNNKLTATAALLTRLRFFHDLGFPYEKALGLSSEMTDQLMCSY 438

Query: 428 LIDQHYDRTPGGLILLRPDDGAPLQFAATASFLSKLYSDYLDLLGASYMSCIFANPGFSL 487
           L +Q+++RTPGGLILLRPD G PLQFAATASFLSKLY DYL LLG S  +C     GFSL
Sbjct: 439 LSEQNFNRTPGGLILLRPDYGEPLQFAATASFLSKLYKDYLTLLGRSGGNCT-KYDGFSL 498

Query: 488 EKLRSFSNSQASAVL 494
           E L+SFS SQ + +L
Sbjct: 499 EMLQSFSISQVNYIL 500

BLAST of CSPI01G21770 vs. ExPASy TrEMBL
Match: A0A061FQ48 (Endoglucanase OS=Theobroma cacao OX=3641 GN=TCM_035466 PE=3 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 2.2e-152
Identity = 291/495 (58.79%), Postives = 361/495 (72.93%), Query Frame = 0

Query: 8   VHTEHEADRFL------STTRLNFNAFSESSIEYNSIPSPYSKSFDFKIVISNQRRFKWC 67
           VHT  EA R L      ++  L+F    +SS  Y+S+PS YSKS+D+++VI+++  +K  
Sbjct: 19  VHTISEAGRLLPSASKWNSIELDFKVLPQSSTGYDSLPSSYSKSYDYELVITDKTHYKRF 78

Query: 68  SYISALLLLLIMALTLLLQFLPHKHNLHEASNNYTVAKSSLTKNVVMNFSCPTDKKLFSA 127
            YIS+ +  LI+AL L+L FLP K++ HE++ N      SL  N  + F          +
Sbjct: 79  LYISSTVAFLILALGLVLHFLPRKNHHHESAKNL-----SLAVNQAITFF-----DAQKS 138

Query: 128 GRYPKSSPVKFRGDSGLEDGVSSNKPDGLIGGFYDSGNNIKFTFPTAYTITLLSWSVIEY 187
           G YP  SP+KFRG SGL DG + N    L+GGFYDSGNNIKFTFP AYTITLLSWSVIEY
Sbjct: 139 GNYPSKSPIKFRGSSGLRDGNTGNTRADLVGGFYDSGNNIKFTFPAAYTITLLSWSVIEY 198

Query: 188 HPKYADMNELDHVKDIIRWGTEYLLKIFVAPNATSDQTIIYSQVGSSSNDS-NAQTND-N 247
           H KY D+ EL+H+KD+IRWG++YLLK+FVAPNATS+ TI+YSQVGS+ ND+ N  +ND N
Sbjct: 199 HQKYEDIGELEHIKDVIRWGSDYLLKVFVAPNATSEPTILYSQVGSAGNDTQNPGSNDIN 258

Query: 248 CWQRPENMMYPRPISTCDARASDLAGEIVAALSASSLVFREDTNYSRELAKAAEKLFQQV 307
           CWQRPE+M Y RP+S CD  ASDLAGEIVAALSA+S+VF+E+  YS+ L KAAEKL+   
Sbjct: 259 CWQRPEDMNYERPVSVCDETASDLAGEIVAALSAASIVFKEENEYSQRLTKAAEKLYGIT 318

Query: 308 TRLDPIEQG-TYSSVDSCGGEARKFYNSSSYTDELIWAGTWLFFATGNTSYLSYATDAVR 367
            + D I +  TY+++D+CGGEARKFYNSS Y DEL+W GTWLFFATGN +YL YAT    
Sbjct: 319 EKKDKIHKAVTYTTIDACGGEARKFYNSSGYKDELVWGGTWLFFATGNYTYLDYAT--TN 378

Query: 368 FQLAQSEEASIGRGIFNWNNKFSATAVLLTRLLYFHDTGYPYEYALGVSSNMTEILMCSY 427
           F  A + E    +GIF WNNK +ATA LLTRL +FHD G+PYE ALG+SS MT+ LMCSY
Sbjct: 379 FAAASNNETIADKGIFYWNNKLTATAALLTRLRFFHDLGFPYEKALGLSSKMTDQLMCSY 438

Query: 428 LIDQHYDRTPGGLILLRPDDGAPLQFAATASFLSKLYSDYLDLLGASYMSCIFANPGFSL 487
           L  Q+++RTPGGLILLRPD G PLQFAATASFLSKLY DYL LLG S  +C   + GFSL
Sbjct: 439 LSKQNFNRTPGGLILLRPDYGEPLQFAATASFLSKLYKDYLTLLGRSGGNCTKCD-GFSL 498

Query: 488 EKLRSFSNSQASAVL 494
           E L+SFS SQ + +L
Sbjct: 499 EMLQSFSISQVNYIL 500

BLAST of CSPI01G21770 vs. ExPASy TrEMBL
Match: A0A6P6ANB4 (Endoglucanase OS=Durio zibethinus OX=66656 GN=LOC111311196 PE=3 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 5.5e-151
Identity = 287/497 (57.75%), Postives = 357/497 (71.83%), Query Frame = 0

Query: 6   RSVHTEHEADRFLSTT------RLNFNAFSESSIEYNSIPSPYSKSFDFKIVISNQRRFK 65
           R VH   E+ R LS+         +FN    SS    S+PSPYSKS+D+++VI+++  +K
Sbjct: 14  RFVHASSESSRLLSSASKRNSIEFDFNVRPSSSTGNGSLPSPYSKSYDYELVITDKTYYK 73

Query: 66  WCSYISALLLLLIMALTLLLQFLPHKHNLHEASNNYTVAKSSLTKNVVMNFSCPTDKKLF 125
              YIS  +  +I+A+ LL  FLPHK+N H  S N T+A        +  F         
Sbjct: 74  RFLYISLTVAFVILAIGLLPHFLPHKNNQHGPSKNLTLA----VNQAITFFDAQ------ 133

Query: 126 SAGRYPKSSPVKFRGDSGLEDGVSSNKPDGLIGGFYDSGNNIKFTFPTAYTITLLSWSVI 185
            +G YP +SP++FRG SGL+DG  SN P  L+GGFYDSGNNIKFTFPTAYTITLLSWSVI
Sbjct: 134 KSGNYPSNSPIRFRGRSGLQDGNLSNTPADLVGGFYDSGNNIKFTFPTAYTITLLSWSVI 193

Query: 186 EYHPKYADMNELDHVKDIIRWGTEYLLKIFVAPNATSDQTIIYSQVGSSSNDS-NAQTND 245
           EYH KYAD+ EL H+KD+I+WG++YLLK+F+APNATSD TI+YSQVGS+ NDS N+  ND
Sbjct: 194 EYHQKYADIGELGHIKDVIKWGSDYLLKVFIAPNATSDPTILYSQVGSAGNDSQNSVPND 253

Query: 246 -NCWQRPENMMYPRPISTCDARASDLAGEIVAALSASSLVFREDTNYSRELAKAAEKLFQ 305
            NCWQRPE M Y RP+S CDA ASDLAGEIVAAL+A+S+VF+E+  YS+ L KAA+KL++
Sbjct: 254 INCWQRPEEMSYKRPVSVCDATASDLAGEIVAALAAASIVFKEENEYSQGLIKAAKKLYE 313

Query: 306 QVTRLDPIEQ-GTYSSVDSCGGEARKFYNSSSYTDELIWAGTWLFFATGNTSYLSYATDA 365
              + D I +  TY+++D+CGGEARKFYNSS Y DEL+W  TWLFFATGN +YL YAT  
Sbjct: 314 ITEKEDQIHKAATYTTIDACGGEARKFYNSSGYKDELVWGETWLFFATGNYTYLDYAT-- 373

Query: 366 VRFQLAQSEEASIGRGIFNWNNKFSATAVLLTRLLYFHDTGYPYEYALGVSSNMTEILMC 425
             F  A + E    +GIF WNNK +A  VLLTRL +F D G+PYE ALG+SSNMT+ +MC
Sbjct: 374 TNFASAANNETIDDKGIFYWNNKLTANTVLLTRLRFFRDLGFPYEEALGISSNMTDHIMC 433

Query: 426 SYLIDQHYDRTPGGLILLRPDDGAPLQFAATASFLSKLYSDYLDLLGASYMSCIFANPGF 485
           SYL +Q++ RTPGGLILLRPD   PLQFAATASFLSKLY+DYL LL  S  +C   N  F
Sbjct: 434 SYLSEQNFYRTPGGLILLRPDYSGPLQFAATASFLSKLYNDYLTLLHRSGWNC--TNDAF 493

Query: 486 SLEKLRSFSNSQASAVL 494
           SLE L+SFS SQ + +L
Sbjct: 494 SLEMLQSFSTSQVNYIL 496

BLAST of CSPI01G21770 vs. NCBI nr
Match: KAE8653204.1 (hypothetical protein Csa_019838 [Cucumis sativus])

HSP 1 Score: 928.3 bits (2398), Expect = 2.8e-266
Identity = 471/496 (94.96%), Postives = 475/496 (95.77%), Query Frame = 0

Query: 1   MMQPERSVHTEHEADRFLSTTRLNFNAFSESSIEYNSIPSPYSKSFDFKIVISNQRRFKW 60
           MMQPERSVHTEHEADRFLSTTRLNFNAFSESSIEYNSIPSPYSKSFDFKIVISNQRRFKW
Sbjct: 1   MMQPERSVHTEHEADRFLSTTRLNFNAFSESSIEYNSIPSPYSKSFDFKIVISNQRRFKW 60

Query: 61  CSYISALLLLLIMALTLLLQFLPHKHNLHEASNNYTVAKSSLTKNVVMNFSCPTDKKLFS 120
           CSYISALLLLLIMALTLLLQFLPHKHNLHEASNNYTVA                   LFS
Sbjct: 61  CSYISALLLLLIMALTLLLQFLPHKHNLHEASNNYTVA-------------------LFS 120

Query: 121 AGRYPKSSPVKFRGDSGLEDGVSSNKPDGLIGGFYDSGNNIKFTFPTAYTITLLSWSVIE 180
           AGRYPKSSPVKFRGDSGLEDGVSSNKPDGLIGGFYDSGNNIKFTFPTAYTITLLSWSVIE
Sbjct: 121 AGRYPKSSPVKFRGDSGLEDGVSSNKPDGLIGGFYDSGNNIKFTFPTAYTITLLSWSVIE 180

Query: 181 YHPKYADMNELDHVKDIIRWGTEYLLKIFVAPNATSDQTIIYSQVGSSSNDSNAQTNDNC 240
           YHPKYADMNELDHVKDIIRWGTEYLLKIFVAPNATSDQTIIYSQVGSSSNDSNAQTNDNC
Sbjct: 181 YHPKYADMNELDHVKDIIRWGTEYLLKIFVAPNATSDQTIIYSQVGSSSNDSNAQTNDNC 240

Query: 241 WQRPENMMYPRPISTCDARASDLAGEIVAALSASSLVFREDTNYSRELAKAAEKLFQQVT 300
           WQRPE+MMYPRPISTCDARASDLAGEIVAALSASSLVFREDTNYSRELAKAAEKLFQQVT
Sbjct: 241 WQRPEDMMYPRPISTCDARASDLAGEIVAALSASSLVFREDTNYSRELAKAAEKLFQQVT 300

Query: 301 RLDPIEQGTYSSVDSCGGEARKFYNSSSYTDELIWAGTWLFFATGNTSYLSYATDAVRFQ 360
           +LDPIEQGTYSSVDSCGGEARKFYNSSSYTDELIWAGTWLFFATGNTSYLSYATDAVRFQ
Sbjct: 301 KLDPIEQGTYSSVDSCGGEARKFYNSSSYTDELIWAGTWLFFATGNTSYLSYATDAVRFQ 360

Query: 361 LAQSEEASIGRGIFNWNNKFSATAVLLTRLLYFHDTGYPYEYALGVSSNMTEILMCSYLI 420
           LAQSEEASIGRGIFNWNNKFSATAVLLTRLLYFHDTGYPYEYALGVSSNMTEILMCSYLI
Sbjct: 361 LAQSEEASIGRGIFNWNNKFSATAVLLTRLLYFHDTGYPYEYALGVSSNMTEILMCSYLI 420

Query: 421 DQHYDRTPGGLILLRPDDGAPLQFAATASFLSKLYSDYLDLLGASYMSCIFANPGFSLEK 480
           DQHYDRTPGGLILLRPDDGAPLQFAATASFLSKLYSDYLDLLGASYMSCIFANPGFSLEK
Sbjct: 421 DQHYDRTPGGLILLRPDDGAPLQFAATASFLSKLYSDYLDLLGASYMSCIFANPGFSLEK 477

Query: 481 LRSFSNSQASAVLRII 497
           LRSFSNSQASA+  I+
Sbjct: 481 LRSFSNSQASALNYIL 477

BLAST of CSPI01G21770 vs. NCBI nr
Match: KAA0057069.1 (endoglucanase 25 [Cucumis melo var. makuwa])

HSP 1 Score: 847.8 bits (2189), Expect = 4.8e-242
Identity = 431/476 (90.55%), Postives = 444/476 (93.28%), Query Frame = 0

Query: 21  TRLNFNAFSESSIEYNSIPSPYSKSFDFKIVISNQRRFKWCSYISALLLLLIMALTLLLQ 80
           TR NFN FS+SS EYNSIPSPYSKSFDFKIVISNQRRFK CSYISALLLLLI+ALTLLLQ
Sbjct: 2   TRHNFNVFSKSSTEYNSIPSPYSKSFDFKIVISNQRRFKCCSYISALLLLLIIALTLLLQ 61

Query: 81  FLPHKHNLHEASNNYTVAKSSLTKNVVMNFSCPTDKKLFSAGRYPKSSPVKFRGDSGLED 140
           FLPHKHNLHEASNNYTVA                   LFSAGRYPKSSPVKFRGDSGL+D
Sbjct: 62  FLPHKHNLHEASNNYTVA-------------------LFSAGRYPKSSPVKFRGDSGLKD 121

Query: 141 GVSSNKPDGLIGGFYDSGNNIKFTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDIIRW 200
           GVSSNKPDGLIGGFYDSGNN+KFTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDIIRW
Sbjct: 122 GVSSNKPDGLIGGFYDSGNNMKFTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDIIRW 181

Query: 201 GTEYLLKIFVAPNATSDQTIIYSQVGSSSNDSNAQTNDNCWQRPENMMYPRPISTCDARA 260
           GTEYLLK+FVAPNATSDQTIIYSQVGSSSN+S AQTNDNCWQRPE+MMYPRP+STCDARA
Sbjct: 182 GTEYLLKVFVAPNATSDQTIIYSQVGSSSNESKAQTNDNCWQRPEDMMYPRPVSTCDARA 241

Query: 261 SDLAGEIVAALSASSLVFREDTNYSRELAKAAEKLFQQVTRLDPIEQGTYSSVDSCGGEA 320
           SDLAGEIVAALSASSLVFREDTNYS ELAKAAEKLFQQVT+LDPIEQGTYSSVDSCGGEA
Sbjct: 242 SDLAGEIVAALSASSLVFREDTNYSGELAKAAEKLFQQVTKLDPIEQGTYSSVDSCGGEA 301

Query: 321 RKFYNSSSYTDELIWAGTWLFFATGNTSYLSYATDAVRFQLAQSEEASIGRGIFNWNNKF 380
           RKFYNSSSYTDELIWAGTWLFFATGNTSYLSYATDAVRFQLAQSEEASIGRGIFNWNNKF
Sbjct: 302 RKFYNSSSYTDELIWAGTWLFFATGNTSYLSYATDAVRFQLAQSEEASIGRGIFNWNNKF 361

Query: 381 SATAVLLTRLLYFHDTGYPYEYALGVSSNMTEILMCSYLIDQHYDRTPGGLILLRPDDGA 440
           SATAVLLTRLLYFHDTGYPYEYALGVSSNMTEILMCSYLIDQH++RTP GLILL PDD A
Sbjct: 362 SATAVLLTRLLYFHDTGYPYEYALGVSSNMTEILMCSYLIDQHFNRTPSGLILLSPDDKA 421

Query: 441 PLQFAATASFLSKLYSDYLDLLGASYMSCIFANPGFSLEKLRSFSNSQASAVLRII 497
           PLQFAATASFLSKLYSDYLDLLGASYMSCIFANP FSLEKLRSFS SQASA+  I+
Sbjct: 422 PLQFAATASFLSKLYSDYLDLLGASYMSCIFANPRFSLEKLRSFSKSQASALNYIL 458

BLAST of CSPI01G21770 vs. NCBI nr
Match: XP_022140170.1 (endoglucanase 25-like [Momordica charantia])

HSP 1 Score: 793.9 bits (2049), Expect = 8.3e-226
Identity = 395/492 (80.28%), Postives = 435/492 (88.41%), Query Frame = 0

Query: 1   MMQPERSVHTEHEADRFLSTTRLNFNAFSESSIEYNSIPSPYSKSFDFKIVISNQRRFKW 60
           MMQP R VH EHEA+R LS+TRL+ N    +SI Y+SIPSPYSKSFDFK+VISN+ RF+W
Sbjct: 1   MMQPVRPVHAEHEANRLLSSTRLDSNVSPRASIGYDSIPSPYSKSFDFKMVISNRTRFRW 60

Query: 61  CSYISALLLLLIMALTLLLQFLPHKHNLHEASNNYTVAKSSLTKNVVMNFSCPTDKKLFS 120
           CSYISALLLLLI+A++ LL FLPHKHN HEASNN+TVA +   K                
Sbjct: 61  CSYISALLLLLIIAVSFLLHFLPHKHNHHEASNNHTVAMNQALKFFDAQ----------K 120

Query: 121 AGRYPKSSPVKFRGDSGLEDGVSSNKPDGLIGGFYDSGNNIKFTFPTAYTITLLSWSVIE 180
           +GRYPK+SPVKFRGDSGLEDGV  NK DGL+GGFYDSGNNIKFTFPTAYTITLLSWSVIE
Sbjct: 121 SGRYPKNSPVKFRGDSGLEDGVVDNKLDGLVGGFYDSGNNIKFTFPTAYTITLLSWSVIE 180

Query: 181 YHPKYADMNELDHVKDIIRWGTEYLLKIFVAPNATSDQTIIYSQVGSSSNDSNAQTNDNC 240
           YHPKYADMNELDHV+DIIRWGT+YLLK+FVAPN TSDQ IIYSQVGS+SNDSN QTNDNC
Sbjct: 181 YHPKYADMNELDHVEDIIRWGTDYLLKVFVAPNGTSDQAIIYSQVGSASNDSNVQTNDNC 240

Query: 241 WQRPENMMYPRPISTCDARASDLAGEIVAALSASSLVFREDTNYSRELAKAAEKLFQQVT 300
           WQRPE+  YPRP+S CD RASDLAGEIVAALSA+SLVF+ED NYS ELAKAAEKLF++VT
Sbjct: 241 WQRPEDTRYPRPVSKCDTRASDLAGEIVAALSAASLVFKEDNNYSGELAKAAEKLFEEVT 300

Query: 301 RLDPIEQGTYSSVDSCGGEARKFYNSSSYTDELIWAGTWLFFATGNTSYLSYATDAVRFQ 360
           +LDP EQGTY+ VDSCGGEAR FYNSSSY DELIWAGTWLF+ATGNTSYL+YATDAVRFQ
Sbjct: 301 KLDPSEQGTYTLVDSCGGEARNFYNSSSYMDELIWAGTWLFYATGNTSYLAYATDAVRFQ 360

Query: 361 LAQSEEASIGRGIFNWNNKFSATAVLLTRLLYFHDTGYPYEYALGVSSNMTEILMCSYLI 420
           LAQS+E+SI RGIF+WNNKFSATAVLLTRLLYFHD  YPYE+ALG SSN T+ILMCSYLI
Sbjct: 361 LAQSKESSIDRGIFDWNNKFSATAVLLTRLLYFHDIVYPYEHALGASSNKTDILMCSYLI 420

Query: 421 DQHYDRTPGGLILLRPDDGAPLQFAATASFLSKLYSDYLDLLGASYMSCIFANPGFSLEK 480
           DQH++RTPGGLI+LRPD GAPLQFAATASFLSKLYSDYLDLLGASYMSCIFANPGFSLEK
Sbjct: 421 DQHFNRTPGGLIILRPDGGAPLQFAATASFLSKLYSDYLDLLGASYMSCIFANPGFSLEK 480

Query: 481 LRSFSNSQASAV 493
           LR+FS SQASA+
Sbjct: 481 LRTFSRSQASAI 482

BLAST of CSPI01G21770 vs. NCBI nr
Match: XP_031745535.1 (endoglucanase 9-like [Cucumis sativus])

HSP 1 Score: 770.4 bits (1988), Expect = 9.8e-219
Identity = 380/387 (98.19%), Postives = 385/387 (99.48%), Query Frame = 0

Query: 107 VMNFSCPTDKKLFSAGRYPKSSPVKFRGDSGLEDGVSSNKPDGLIGGFYDSGNNIKFTFP 166
           +MNFSCPTDKKLFSAGRYPKSSPVKFRGDSGLEDGVSSNKPDGLIGGFYDSGNNIKFTFP
Sbjct: 1   MMNFSCPTDKKLFSAGRYPKSSPVKFRGDSGLEDGVSSNKPDGLIGGFYDSGNNIKFTFP 60

Query: 167 TAYTITLLSWSVIEYHPKYADMNELDHVKDIIRWGTEYLLKIFVAPNATSDQTIIYSQVG 226
           TAYTITLLSWSVIEYHPKYADMNELDHVKDIIRWGTEYLLKIFVAPNATSDQTIIYSQVG
Sbjct: 61  TAYTITLLSWSVIEYHPKYADMNELDHVKDIIRWGTEYLLKIFVAPNATSDQTIIYSQVG 120

Query: 227 SSSNDSNAQTNDNCWQRPENMMYPRPISTCDARASDLAGEIVAALSASSLVFREDTNYSR 286
           SSSNDSNAQTNDNCWQRPE+MMYPRPISTCDARASDLAGEIVAALSASSLVFREDTNYSR
Sbjct: 121 SSSNDSNAQTNDNCWQRPEDMMYPRPISTCDARASDLAGEIVAALSASSLVFREDTNYSR 180

Query: 287 ELAKAAEKLFQQVTRLDPIEQGTYSSVDSCGGEARKFYNSSSYTDELIWAGTWLFFATGN 346
           ELAKAAEKLFQQVT+LDPIEQGTYSSVDSCGGEARKFYNSSSYTDELIWAGTWLFFATGN
Sbjct: 181 ELAKAAEKLFQQVTKLDPIEQGTYSSVDSCGGEARKFYNSSSYTDELIWAGTWLFFATGN 240

Query: 347 TSYLSYATDAVRFQLAQSEEASIGRGIFNWNNKFSATAVLLTRLLYFHDTGYPYEYALGV 406
           TSYLSYATDAVRFQLAQSEEASIGRGIFNWNNKFSATAVLLTRLLYFHDTGYPYEYALGV
Sbjct: 241 TSYLSYATDAVRFQLAQSEEASIGRGIFNWNNKFSATAVLLTRLLYFHDTGYPYEYALGV 300

Query: 407 SSNMTEILMCSYLIDQHYDRTPGGLILLRPDDGAPLQFAATASFLSKLYSDYLDLLGASY 466
           SSNMTEILMCSYLIDQHYDRTPGGLILLRPDDGAPLQFAATASFLSKLYSDYLDLLGASY
Sbjct: 301 SSNMTEILMCSYLIDQHYDRTPGGLILLRPDDGAPLQFAATASFLSKLYSDYLDLLGASY 360

Query: 467 MSCIFANPGFSLEKLRSFSNSQASAVL 494
           MSCIFANPGFSLEKLRSFSNSQ + +L
Sbjct: 361 MSCIFANPGFSLEKLRSFSNSQLNYIL 387

BLAST of CSPI01G21770 vs. NCBI nr
Match: KAG6573332.1 (Endoglucanase 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 755.7 bits (1950), Expect = 2.5e-214
Identity = 377/486 (77.57%), Postives = 423/486 (87.04%), Query Frame = 0

Query: 1   MMQPERSVHTEH-EADRFLSTTRLNFNAFSESSIEYNSIPSPYSKSFDFKIVISNQRRFK 60
           MMQPER VH EH EAD  +ST R++FN  S S++ Y+SI SPY KS DFKIVISN+ RFK
Sbjct: 1   MMQPERFVHAEHDEADYLVSTARVSFNVSSTSTVVYDSIRSPYYKSIDFKIVISNRTRFK 60

Query: 61  WCSYISALLLLLIMALTLLLQFLPHKHNLHEASNNYTVAKSSLTKNVVMNFSCPTDKKLF 120
           WCSYISA +LL+I+AL LLL FLPHKH+ HEASNN+TVA        +  F         
Sbjct: 61  WCSYISAFVLLVIIALALLLNFLPHKHDSHEASNNHTVA----VHQALEFFDAQ------ 120

Query: 121 SAGRYPKSSPVKFRGDSGLEDGVSSNKPDGLIGGFYDSGNNIKFTFPTAYTITLLSWSVI 180
            +GRYP++SPV FRGDSGL+DGVSS+KPDGL+GGFYDSGNNIKFTFPTAYTITLL WSVI
Sbjct: 121 KSGRYPENSPVDFRGDSGLDDGVSSSKPDGLVGGFYDSGNNIKFTFPTAYTITLLGWSVI 180

Query: 181 EYHPKYADMNELDHVKDIIRWGTEYLLKIFVAPNATSDQTIIYSQVGSSSNDSNAQTNDN 240
           EYHPKYADMNELDHVKDII+WGT+YLLK+FVAPN+TSD+TIIYSQVGS SNDS AQ NDN
Sbjct: 181 EYHPKYADMNELDHVKDIIKWGTDYLLKVFVAPNSTSDRTIIYSQVGSVSNDSKAQNNDN 240

Query: 241 CWQRPENMMYPRPISTCDARASDLAGEIVAALSASSLVFREDTNYSRELAKAAEKLFQQV 300
           CWQRPE+M Y RP+S CDARASDLAGE+VAALSA+SLVF+ED NYS ELAKA EKLF+QV
Sbjct: 241 CWQRPEDMRYTRPVSECDARASDLAGEVVAALSAASLVFKEDNNYSGELAKAVEKLFEQV 300

Query: 301 TRLDPIEQGTYSSVDSCGGEARKFYNSSSYTDELIWAGTWLFFATGNTSYLSYATDAVRF 360
           T+LDP EQGTY+ VD CGGEAR FYNSS + DELIWAG+WLFFATGN SYL+Y+TDAVRF
Sbjct: 301 TKLDPSEQGTYTLVDLCGGEARNFYNSSGFMDELIWAGSWLFFATGNASYLAYSTDAVRF 360

Query: 361 QLAQSEEASIGRGIFNWNNKFSATAVLLTRLLYFHDTGYPYEYALGVSSNMTEILMCSYL 420
           QLA++E ASI +GIF+WNNKFSATAVLLTRLLYFHD GYPYE+ L  SSNMTEILMCSYL
Sbjct: 361 QLARTEVASIDQGIFDWNNKFSATAVLLTRLLYFHDIGYPYEHVLRASSNMTEILMCSYL 420

Query: 421 IDQHYDRTPGGLILLRPDDGAPLQFAATASFLSKLYSDYLDLLGASYMSCIFANPGFSLE 480
           I+QH++RTPGGLILLRP DGAPLQFAATASFLSKLYSDYLDLLGASYMSCIFANPGFSLE
Sbjct: 421 IEQHFNRTPGGLILLRPADGAPLQFAATASFLSKLYSDYLDLLGASYMSCIFANPGFSLE 476

Query: 481 KLRSFS 486
           KL++FS
Sbjct: 481 KLKAFS 476

BLAST of CSPI01G21770 vs. TAIR 10
Match: AT5G49720.1 (glycosyl hydrolase 9A1 )

HSP 1 Score: 302.8 bits (774), Expect = 5.4e-82
Identity = 187/460 (40.65%), Postives = 265/460 (57.61%), Query Frame = 0

Query: 44  KSFDFKIVISNQRRFKWCSYISALLLLLIMALTLLLQFLPHKHNLHEASNNYTVAKSSLT 103
           K  D   +I +++ F W         LL   +TL+++ +P  H      +NYT+A   L 
Sbjct: 58  KYVDLGCIIVSRKIFVWTVGTLVAAALLAGFITLIVKTVPRHHPKTPPPDNYTIA---LH 117

Query: 104 KNVVMNFSCPTDKKLFSA---GRYPKSSPVKFRGDSGLEDGVSSNKP--DGLIGGFYDSG 163
           K +          K F+A   G+ PK + V +RG+SGL+DG          L+GG+YD+G
Sbjct: 118 KAL----------KFFNAQKSGKLPKHNNVSWRGNSGLQDGKGETGSFYKDLVGGYYDAG 177

Query: 164 NNIKFTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDIIRWGTEYLLKIFVAPNATSDQ 223
           + IKF FP AY +T+LSWSVIEY  KY    EL HVK++I+WGT+Y LK F   N+T+D 
Sbjct: 178 DAIKFNFPMAYAMTMLSWSVIEYSAKYEAAGELTHVKELIKWGTDYFLKTF---NSTADS 237

Query: 224 -TIIYSQVGS-SSNDSNAQTNDN-CWQRPENMMYPRPISTCDARASDLAGEIVAALSASS 283
              + SQVGS +++D N   ND+ CW RPE+M Y RP++TC+   SDLA E+ AAL+++S
Sbjct: 238 IDDLVSQVGSGNTDDGNTDPNDHYCWMRPEDMDYKRPVTTCNGGCSDLAAEMAAALASAS 297

Query: 284 LVFREDTNYSRELAKAAEKLFQQVTRLDPIEQGTYSSVDSCGGEARKFYNSSSYTDELIW 343
           +VF+++  YS++L   A+ ++Q         +G YS+  +   E+ KFYNSS Y DE IW
Sbjct: 298 IVFKDNKEYSKKLVHGAKVVYQ----FGRTRRGRYSAGTA---ESSKFYNSSMYWDEFIW 357

Query: 344 AGTWLFFATGNTSYLSYATDAVRFQLAQSEEASIGRGIFNWNNKFSATAVLLTRLLYFHD 403
            G W+++ATGN +YL+  T     + A +       G+F+W+NK +   +LL+RL  F  
Sbjct: 358 GGAWMYYATGNVTYLNLITQPTMAKHAGAFWGGPYYGVFSWDNKLAGAQLLLSRLRLFLS 417

Query: 404 TGYPYEYALGVSSNMTEILMCSYL-IDQHYDRTPGGLILLRPDDGAPLQFAATASFLSKL 463
            GYPYE  L    N T I+MCSYL I   ++RT GGLI L      PLQ++  A+FL+ L
Sbjct: 418 PGYPYEEILRTFHNQTSIVMCSYLPIFNKFNRTNGGLIELNHGAPQPLQYSVNAAFLATL 477

Query: 464 YSDYLDLLGASYMSCIFANPGF-SLEKLRSFSNSQASAVL 494
           YSDYLD        C    P F S   LR F+ SQ   +L
Sbjct: 478 YSDYLDAADTPGWYC---GPNFYSTSVLRDFARSQIDYIL 491

BLAST of CSPI01G21770 vs. TAIR 10
Match: AT4G24260.1 (glycosyl hydrolase 9A3 )

HSP 1 Score: 282.7 bits (722), Expect = 5.8e-76
Identity = 179/461 (38.83%), Postives = 258/461 (55.97%), Query Frame = 0

Query: 44  KSFDFKIVISNQRRFKWCSYISALLLLLIMALTLLLQFLPHKHNLHEASNNYTVAKSSLT 103
           K  D   ++ +++ F W      +  LL   +TL+++ LPH H+     +NYT+A     
Sbjct: 58  KYVDLGCILVSRKIFLWTLGTIVVTALLSGFITLIVKTLPHHHHKEPPPDNYTIA----- 117

Query: 104 KNVVMNFSCPTDKKLFSA---GRYPKS-SPVKFRGDSGLEDGVSSNKP--DGLIGGFYDS 163
                     T  K F+A   G+ PK+   V +R DS L+DG          L+GG+YD+
Sbjct: 118 --------LRTALKFFNAQQSGKLPKNIYNVSWRHDSCLQDGKGDPGQCYKDLVGGYYDA 177

Query: 164 GNNIKFTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDIIRWGTEYLLKIFVAPNATSD 223
           G++IKF FP +Y +T+LSWSVIEY  KY    EL+HVK++I+WGT+Y LK F   N+++D
Sbjct: 178 GDSIKFNFPMSYAMTMLSWSVIEYSAKYQAAGELEHVKELIKWGTDYFLKTF---NSSAD 237

Query: 224 QT-IIYSQVGS--SSNDSNAQTNDNCWQRPENMMYPRPISTCDARASDLAGEIVAALSAS 283
              ++  QVGS  S   S    +  CW RPE++ Y R +S C +  SDLA E+ AAL+++
Sbjct: 238 NIYVMVEQVGSGVSGRGSELHNDHYCWMRPEDIHYKRTVSQCYSSCSDLAAEMAAALASA 297

Query: 284 SLVFREDTNYSRELAKAAEKLFQQVTRLDPIEQGTYSSVDSCGGEARKFYNSSSYTDELI 343
           S+VF+++  YS+ L   A+ L++  T      +  YS     G E+ KFYNSS + DEL+
Sbjct: 298 SIVFKDNRLYSKNLVHGAKTLYRFAT----TSRNRYS---QNGKESSKFYNSSMFEDELL 357

Query: 344 WAGTWLFFATGNTSYLSYATDAVRFQLAQSEEASIGRGIFNWNNKFSATAVLLTRLLYFH 403
           W G WL++ATGN +YL   T     + A +   S   G+F+W+NK     +LLTR+  F 
Sbjct: 358 WGGAWLYYATGNVTYLERVTSHHMAEKAGAFGNSPYYGVFSWDNKLPGAQLLLTRMRLFL 417

Query: 404 DTGYPYEYALGVSSNMTEILMCSYL-IDQHYDRTPGGLILLRPDDGAPLQFAATASFLSK 463
             GYPYE  L    N T  +MCSYL   + ++RT GGLI L      PLQ+ A A+FL+ 
Sbjct: 418 SPGYPYEDMLSEFHNQTGRVMCSYLPYYKKFNRTNGGLIQLNHGAPQPLQYVANAAFLAA 477

Query: 464 LYSDYLDLLGASYMSCIFANPGF-SLEKLRSFSNSQASAVL 494
           L+SDYL+        C    P F + E LR+FS SQ   +L
Sbjct: 478 LFSDYLEAADTPGWYC---GPNFYTTEFLRNFSRSQIDYIL 492

BLAST of CSPI01G21770 vs. TAIR 10
Match: AT1G65610.1 (Six-hairpin glycosidases superfamily protein )

HSP 1 Score: 266.9 bits (681), Expect = 3.3e-71
Identity = 164/444 (36.94%), Postives = 250/444 (56.31%), Query Frame = 0

Query: 58  FKWCSYISALLLLLIMALTLLLQFLPHKHNLHEASNNYTVAKSSLTKNVVMNFSCPTDKK 117
           F W     A+L L++    ++++ LP   +     +NYT+A        +  F       
Sbjct: 80  FLWTVGSIAVLFLVVALPIIIVKSLPRHKSAPPPPDNYTLA----LHKALQFFDAQ---- 139

Query: 118 LFSAGRYPKSSPVKFRGDSGLEDGVSSNKPD---GLIGGFYDSGNNIKFTFPTAYTITLL 177
              +G+ PK + V +RGDSG +DG+    PD   GL+GG+YD G+N+KF FP A+++T+L
Sbjct: 140 --KSGKLPKKNKVSWRGDSGTKDGL----PDVVGGLVGGYYDGGSNVKFHFPMAFSMTML 199

Query: 178 SWSVIEYHPKYADMNELDHVKDIIRWGTEYLLKIFVAPNATSDQTIIYSQVGSSSNDSNA 237
           SWS+IEY  KY  ++E DH++D+++WGT+YLL  F   N+ +    IY+QVG    DS +
Sbjct: 200 SWSLIEYSHKYKAIDEYDHMRDVLKWGTDYLLLTF--NNSATRLDHIYTQVGGGLRDSES 259

Query: 238 QTNDNCWQRPENMMYPRPISTCDARASDLAGEIVAALSASSLVFREDTNYSRELAKAAEK 297
             +  CWQ+PE+M Y RP+ +    A+DL  E+ AAL+A+S+VF +  +Y+++L K AE 
Sbjct: 260 PDDIYCWQKPEDMSYDRPVLS-STSAADLGAEVSAALAAASIVFTDKPDYAKKLKKGAET 319

Query: 298 L---FQQVTRLDPIEQGTYSSVDSCGGEARKFYNSSSYTDELIWAGTWLFFATGNTSYLS 357
           L   F+  +R      G  +        A+ FYNS+S  DE +WAG WL++ATGN +Y+ 
Sbjct: 320 LYPFFRSKSRRKRYSDGQPT--------AQAFYNSTSMFDEFMWAGAWLYYATGNKTYIQ 379

Query: 358 YATDAVRFQLAQSEEASIGRGIFNWNNKFSATAVLLTRLLYFHDTGYPYEYALGVSSNMT 417
           +AT     Q A++        + +WNNK     +L+TR   F + G+PYE  L    N T
Sbjct: 380 FATTPSVPQTAKAFANRPELMVPSWNNKLPGAMLLMTRYRLFLNPGFPYENMLNRYHNAT 439

Query: 418 EILMCSYLIDQH-YDRTPGGLILLRPDDGAPLQFAATASFLSKLYSDYLDLLGASYMSCI 477
            I MC+YL   + ++RT GGL+ L      PL++ A ASFL+ L++DYL+  G     C 
Sbjct: 440 GITMCAYLKQYNVFNRTSGGLMQLNLGKPRPLEYVAHASFLASLFADYLNSTGVPGWYC- 495

Query: 478 FANPGF-SLEKLRSFSNSQASAVL 494
              P F     L+ F+ SQ   +L
Sbjct: 500 --GPTFVENHVLKDFAQSQIDYIL 495

BLAST of CSPI01G21770 vs. TAIR 10
Match: AT1G75680.1 (glycosyl hydrolase 9B7 )

HSP 1 Score: 201.1 bits (510), Expect = 2.2e-51
Identity = 136/375 (36.27%), Postives = 210/375 (56.00%), Query Frame = 0

Query: 126 KSSPVKFRGDSGLEDGVSSNKPDGLIGGFYDSGNNIKFTFPTAYTITLLSWSVIEYHPKY 185
           +++ + +RGDSGL+DG   N    L  G YD+G++IKF FP A+T T+LSWS++EY  + 
Sbjct: 78  ENNKIPWRGDSGLKDGSEDNL--DLSKGLYDAGDHIKFGFPMAFTATVLSWSILEYGDQM 137

Query: 186 ADMNELDHVKDIIRWGTEYLLKIFVAPNATSDQTIIYSQVGSSSNDSNAQTNDNCWQRPE 245
             +N+LD  KD +RW T+YL+K   + N      ++Y QVG      + + +  CW+RPE
Sbjct: 138 NAVNQLDPAKDSLRWITDYLIKAHPSDN------VLYIQVG------DPKVDHPCWERPE 197

Query: 246 NMMYPRPIS--TCDARASDLAGEIVAALSASSLVFRE-DTNYSRELAKAAEKLFQQVTRL 305
           +M   RP++    D   +++A E  AA++++SLVF++ D  YS  L K A++LF      
Sbjct: 198 DMKEKRPLTKIDVDTPGTEVAAETAAAMASASLVFKDSDPTYSATLLKHAKQLF----NF 257

Query: 306 DPIEQGTYSSVDSCGGEARKFYNSSSYTDELIWAGTWLFFATGNTSYLSYATDAVRFQLA 365
              ++G+YS       E +KFYNS+ Y DEL+WA +WL+ AT + +YL Y ++  +    
Sbjct: 258 ADTKRGSYSVNIP---EVQKFYNSTGYGDELLWAASWLYHATEDKTYLDYVSNHGK---- 317

Query: 366 QSEEASIGRGI-FNWNNKFSATAVLLTRLLYFHDTGYPYEYALGVSSNMTEILMCSYL-- 425
             E AS G    F+W+NK + T VLL+RLL+F          LG   N  + +MC  L  
Sbjct: 318 --EFASFGNPTWFSWDNKLAGTQVLLSRLLFF-KKDLSGSKGLGNYRNTAKAVMCGLLPK 377

Query: 426 -IDQHYDRTPGGLILLRPDDGAPLQFAATASFLSKLYSDYLDLLGASYMSCIFANPGFSL 485
                  RT GGLI +   +   +Q + +++FL+ L+SDY+       +SC      F  
Sbjct: 378 SPTSTASRTNGGLIWV--SEWNSMQQSVSSAFLASLFSDYMLTSRIHKISC--DGKIFKA 420

Query: 486 EKLRSFSNSQASAVL 494
            +LR F+ SQA  +L
Sbjct: 438 TELRDFAKSQADYML 420

BLAST of CSPI01G21770 vs. TAIR 10
Match: AT1G19940.1 (glycosyl hydrolase 9B5 )

HSP 1 Score: 199.9 bits (507), Expect = 4.9e-51
Identity = 142/440 (32.27%), Postives = 228/440 (51.82%), Query Frame = 0

Query: 61  CSYISALLLLLIMALTLLLQFLPHKHNLHEASNNYTVAKSSLTKNVVMNFSCPTDKKLFS 120
           CS    +++L+ + + ++   + H+ N  +   +     ++  K  +  F      KL  
Sbjct: 12  CSVFIGVIILIAIIIAVIFT-IRHRSNHSDDDGSNVKNYANALKIAMQFFDIQKSGKL-- 71

Query: 121 AGRYPKSSPVKFRGDSGLEDGVSSNKPDGLIGGFYDSGNNIKFTFPTAYTITLLSWSVIE 180
                +++ + +RGDSGL+DG  S     L  G YD+G+++KF FP A+T T+LSWS++E
Sbjct: 72  -----ENNEISWRGDSGLKDG--SEASIDLSKGLYDAGDHMKFGFPMAFTATVLSWSILE 131

Query: 181 YHPKYADMNELDHVKDIIRWGTEYLLKIFVAPNATSDQTIIYSQVGSSSNDSNAQTNDNC 240
           Y  + A +N LDH KD ++W T++L+    +PN      ++Y QVG      +  T+  C
Sbjct: 132 YGDQMASLNLLDHAKDSLKWTTDFLINAHPSPN------VLYIQVG------DPVTDHKC 191

Query: 241 WQRPENMMYPRPISTCDAR--ASDLAGEIVAALSASSLVFRE-DTNYSRELAKAAEKLFQ 300
           W RPE M   R ++  D +   +++A E  AA++A+SLVF+E DT YS  L K A++LF 
Sbjct: 192 WDRPETMTRKRTLTKIDTKTPGTEVAAETAAAMAAASLVFKESDTKYSSTLLKHAKQLFD 251

Query: 301 QVTRLDPIEQGTYSSVDSCGGEARKFYNSSSYTDELIWAGTWLFFATGNTSYLSYATDAV 360
                    +G+YS       E + +YNS+ Y DEL+WA +WL+ AT + +YL + ++  
Sbjct: 252 FADN----NRGSYSVNIP---EVQSYYNSTGYGDELLWAASWLYHATEDQTYLDFVSE-- 311

Query: 361 RFQLAQSEEASIGR-GIFNWNNKFSATAVLLTRLLYFHDTGYPYEYALGVSSNMTEILMC 420
                  E  + G    F+W+NK   T +LL+RL +F   G      L       E +MC
Sbjct: 312 ----NGEEFGNFGSPSWFSWDNKLPGTHILLSRLTFF-KKGLSGSKGLQGFKETAEAVMC 371

Query: 421 SYLIDQ---HYDRTPGGLILLRPDDGAPLQFAATASFLSKLYSDYLDLLGASYMSCIFAN 480
             +         RT GGLI +   +   LQ   +++FL+ LYSDY+   G   +SC  ++
Sbjct: 372 GLIPSSPTATSSRTDGGLIWV--SEWNALQHPVSSAFLATLYSDYMLTSGVKELSC--SD 411

Query: 481 PGFSLEKLRSFSNSQASAVL 494
             F    LR F+ SQA  +L
Sbjct: 432 QSFKPSDLRKFARSQADYML 411

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q388907.6e-8140.65Endoglucanase 25 OS=Arabidopsis thaliana OX=3702 GN=KOR PE=1 SV=1[more]
P0C1U47.3e-7637.58Endoglucanase 9 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU1 PE=2 SV=1[more]
Q84R494.8e-7539.12Endoglucanase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU2 PE=2 SV=1[more]
Q9STW88.1e-7538.83Endoglucanase 21 OS=Arabidopsis thaliana OX=3702 GN=KOR3 PE=2 SV=1[more]
Q7XUK41.1e-7135.96Endoglucanase 12 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU3 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A5A7UU462.3e-24290.55Endoglucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G002590 ... [more]
A0A6J1CED24.0e-22680.28Endoglucanase OS=Momordica charantia OX=3673 GN=LOC111010902 PE=3 SV=1[more]
A0A6J1ACP61.3e-15259.19Endoglucanase OS=Herrania umbratica OX=108875 GN=LOC110416795 PE=3 SV=1[more]
A0A061FQ482.2e-15258.79Endoglucanase OS=Theobroma cacao OX=3641 GN=TCM_035466 PE=3 SV=1[more]
A0A6P6ANB45.5e-15157.75Endoglucanase OS=Durio zibethinus OX=66656 GN=LOC111311196 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAE8653204.12.8e-26694.96hypothetical protein Csa_019838 [Cucumis sativus][more]
KAA0057069.14.8e-24290.55endoglucanase 25 [Cucumis melo var. makuwa][more]
XP_022140170.18.3e-22680.28endoglucanase 25-like [Momordica charantia][more]
XP_031745535.19.8e-21998.19endoglucanase 9-like [Cucumis sativus][more]
KAG6573332.12.5e-21477.57Endoglucanase 7, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT5G49720.15.4e-8240.65glycosyl hydrolase 9A1 [more]
AT4G24260.15.8e-7638.83glycosyl hydrolase 9A3 [more]
AT1G65610.13.3e-7136.94Six-hairpin glycosidases superfamily protein [more]
AT1G75680.12.2e-5136.27glycosyl hydrolase 9B7 [more]
AT1G19940.14.9e-5132.27glycosyl hydrolase 9B5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001701Glycoside hydrolase family 9PFAMPF00759Glyco_hydro_9coord: 121..471
e-value: 3.3E-66
score: 224.5
IPR012341Six-hairpin glycosidase-like superfamilyGENE3D1.50.10.10coord: 118..500
e-value: 1.1E-79
score: 270.7
NoneNo IPR availablePANTHERPTHR22298ENDO-1,4-BETA-GLUCANASEcoord: 24..492
NoneNo IPR availablePANTHERPTHR22298:SF109ENDOGLUCANASEcoord: 24..492
IPR008928Six-hairpin glycosidase superfamilySUPERFAMILY48208Six-hairpin glycosidasescoord: 121..459

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G21770.1CSPI01G21770.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030245 cellulose catabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008810 cellulase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds