CSPI01G18550 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G18550
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionacyl-protein thioesterase 2
LocationChr1: 13984809 .. 13990100 (+)
RNA-Seq ExpressionCSPI01G18550
SyntenyCSPI01G18550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACGTAGGCTGTTTGAGGGCCCCGATAAACCCAAAAGCAAGACTCATTTTATAGCAAATTCAAGCATATCTTGACACCAATTATTATAATTGAACTTCCTCCAGGTTGAATCGTTCATCGAAGATTAAAAAATCTCAACCTTTCCTAACTTTGGGAGTACCTATCGCTGCAGCCATGGCAAATTAAACACAACAGGTGCGAACCGGGCGGGGCGGGGACCAAACGCACACACCCCTCGAAACAAAATTGTGTGTGAGAGAGAGAGAGAAAGAGAGAGAAACAATCAATACAGCGACGACAGCAACAACGAAAACAAGATATAGCCCATAACCACACCCCACTTCAAACCCCTCTCTCTGGCTCCTCCCCCGCCCAGGTTTGTTTTACTTTCTTTCTCTCAAATCCTCAATACCCCTTTTTTAATTTTTACTCTAGATTCATTTTTTCTTTATGGGTCATTGATGTTTTCCTGTTTTGATTCGTTTTCCTTGGTTTTTTGAAATGGAATACATGATGGGAGATTTTTTTTTCTTTTTCTGAATGTTTTTTTTTACTTGGTTTCTTGAATTTCTTTTGGGGGATGGGATGGTTTGGTGATGGTGAGAAGTGGTTTCTGAATCTTGGGTAGTGTTTTGTTTGTGGGTTTTTTTGAAGAGTTTTGGTTTATTGTGGCTGCTCTTGGGCTGTAGTCATAGGGGAAACTTGTTTTAATTTTGTGGGGGTTGTTTTGAAATGTACGTATTTTTGTGGTCTCTCGTTGTTCATTCTTTCTTCCATGGAGGTTTGAAAGGGGAGTCCAAGGGATTGAAATGAGTTATAATTTCTCAAGTGAAGCCGGGGTCCTTACATTGTTTTTAACGACCTTTGTTATTGGATGATGTTTAACGAGATTTCGTCTTTGGATGATTTTTGATTCAGTAAGTTTCTCAAAATAATTTAAACAGTTTCGAGGTGGCTTATTGTGATTTATTTTCTTTACCCCCAGCAGGATCAATTGATAGTCAATCTCTTAAGGTGGTGGTTTACTTGGCTTTTGTATTGCGAATATGAGTTTTGCTGGCTCTTCTGTTGCTGCTGGTCAGTGTACTTGTCCCTCTGCATTCTCCTGCTGTTGATTAGTTTTCTTCTTTTCTTTTGATTTTTAGATGTTTTATTATATTGTATATCTCATTTTGGATTTATCTCCTTGTCCATCTTTTGAATCCTTAAACATTACTGTTTTATTCAACAAACGATTCTGTATTTCCTGTTTGTGGGAGTAGCTCTGTATCATTCCAATTTTATGGGACGTCCCGAAGGAAGAGTTTTTTTTGGAGATTTTCGCTCTTTCTAGAGGACGTCCCGATGACTTTTTCTTTTCACGGGATAGGGGGAAAAGTTTTGTGGCAGGCTTGTTGTGCTATTTTGTGGGGCGTGTAGGTCGAGTGGAATGGTTGATTTTAAGAGGTCTCGAGAGGTCTTTACTCTTTAGAGTAGCTGTAGGAGAGATCAAGGTTTATTGCATCTTTATGGGTGTTTGCTATTTATTCTTCTTCTTCTTTTTTGTAATTATCAGCTTAGTTTCATTCTTTTAGATGGGAGACCTTTCCTCTCTTGTGGTTTCTTTCTTTGTTTTTCTTTTTCTTTTCTTTTACTACTTATCTATGGTCTTCTTTATTCTTTCATTTTTCTCGATGAAAGCTCGATGTCTTTGAAAAAAAATGATGGGAGTAACTAGCCTCTTTGTTGGCTTCGCTTTAAGATATAATATTTTGTGGAAATTGAGGTATCTTGCTTGAGCATGTCATGGTCACTTTCAGGTGGTAGAGCTGCCAAAAAGGCAATCGAGTTTGGTAAAACCTACGTAGTGAGGCCGAAGGGAAAACACCAAGCTACTGTTGTCTGGCTTCATGGTCTTGGTGACAATGGCTCAAGGTTCTGTTTCTTCTGTTTCTCTTCTTTTCTTCTTCTCTTAAATTTTTTACCTATAGCATCAATTCATCTCCGTAGTTCATCCGCATGTGGATCATTCAGTTAGTCATTTAATTACATAATTTCATTTATATGTATTCAAGTTTCATCACTTCTTGTCTTGGTCTTCACTGACAAATTTTCTTCAAGACTATAATATTTGACCTTTCTACATCAAATGTCAACAAACCTAAAAGCTTAGGCCAATGATTATGAAAAATTTAATATTATATCAACGCTCTAACATTTTTTCTCATTGCGGGTGAAAGTTCATTGAAGGTCTAACAAGTGAAATTCCCTATTAATTAGGGAGGAAATGACTTTACAGGGGTTCAATTCAAACTCGGGACCTTTTGCTTTGATACCTTGTTAAACACTAATTAACCCGAAAACTTGAATAAATGATTATGGTAAATTTAATGTTATATCAACACTTTGACATGGATCATGTTGTCCCTTGTCTTCCTAAATCCACCTAAACTCTAAACCCCACCTAACCTTTTTCTCATTTTGTGTTTCCAATTCAAAGATTGTTTACTCCAATACAAAATCATAGACTCCTTCTAAAGAAATTGAATTTTCGTTGTCTTCTCTGATCTTGTTAGTGTGATATGACAAATGTTCAGTTGTTAGAAGAACAGACCTTTTAAAGTTCCATATCCTATTTCTTTCCAAGTCACCTATATGTTATACAATACGCTCTCTGGGTTGGAGTCCACCGGCAGAGGGAGGGATCATCTTTCATCGTTCCTCAGCAATTGTTTCTTTTGTCCACAAATCCATGCAGGTTGTTTTGTTACTTATAGAAATTGGGGAATGGTGTGATTGAGTTGTTTCCTCAGTTGGAATGTAGAAATTGGGAATGGTATGATTGAGTTGTTTCCTCAGTTGGAAGTATTATATTATTATTGTCGAAGTGGCTTCGTAATCTGATCTTTTATCTCTCTATGAAAAGCTTACTCTGAAATTTTAACATAGCTGTATATAGTTTAGCTTTTTCACCACTTTTCAGTATGATTCATGAGACTGCTAAGGATACTTCTCCTGGTAGATTTTTAATTGAAACTTTTATCTGACAGACTTCCACGTTATGATTATCCTTAGGGTAATACCTTTGAAAAATATAATGGGCATCGTGAAATGTACTATTTTGTTATAAACTTTGCCATTCTATTTAGGAATGTCGAGCTTAGAAATGTTTTGATTTTGTTTTACAGCTGGTCGCAACTATTGGAGACGCTTCCTTTACCAAATGTAAGAAGCATCTATTCAAAAAAAAAAATTCTATAATTTCTTCTCAGTCCCGTTTCATTTTGTTACTCTTTTTAATAATCTAAGAGGTAAAAAATAGATGGAAGAATTCATTGCTATAAGATGAAATGGTATATTTAGTGTTATTTTATAAACTTGGAGTTTCTGATGCAGATTAAATGGATATGTCCGACTGCGCCTACTCGACCAATAGCTTTATTTGGTGGCTTTCCATCCACTGCATGTAAGCTTACTGAAATCCCTTTTTCTTTATATCAGATCATCACTCAACCAGGATCTCTTGTTATTTACGTATTTCACAATAGCAAGGGCATCAAGACTGTTGTCCTACCTGCTCTTCTATGAACCATCTCCTTGCGCCTCCTCTTATATTTGAAGCAAAAGCTTTATTGAAAGAAGCAAAACTCACTTGGACCAACATATATTACAACAGTGTGAAACAAAAGTTACAGTGAGATGTTCTTTTTAAACCAGGGTTTGATGTGGAAGATCTTTCAGAAGATGGTCCAGATGATTTGGAGGGTTTGGATGCTTCAGCCGCACATGTTGCATATTTGTTATCAACAGAGCCAGCTGACAGTATGTTTCTCTTGCAATTAATGTCTGGCTATCATTGATCCAACGTTTTCTTTAAATCCTAAGATAGAGTTTCAGTAGCATTTTAAGTGATTAGACTACTTTAACTCCTATTTCTTTTGCCTCTTCCATTGATTGTTGGTATAATTCTGCTGTAATCTTTATTCTTTCCTACAGTTAAGCTTGGTGTAGGAGGGTTCAGTATGGGCGCAGCTACTGCGCTTTACTCCGCAACCTGCCACGCGGTGGGAAAATACGGGAACGGAAATCCTTATCCAGCCAATCTGAGTGCAGTTGTTGGGCTTAGTGGCTGGCTTCCATGTTCAAAGTTGGTTTCTTAATCTTGTCATCTGGACAACCTTTGATTCTTTTACAATCAATACGAAGTTTCATTTAACGGGTTATCATGGTGATTCCAGGACTTTGAAAACCAATATGGAACAGAAGAACGCCGGTAATAGTGGAGCAGGATCCTTGCCTATTTTGCTATGCCATGGAAAAGGTATTTAATTTATAAGTTTATAATTGTTCTGCAGAAGATGGTTATTTTTTTTTACAGATCCTCTGTTCTATGGTTCGGCTGAATTTTACTATACAAATTTCAGTTTGTTGTGTCTTTACGGCTTATACTTTCACCTTAATTATGGTTTATCTGTTCATAAGACGATGGGTGTTATTGTCAATTTATCTTCGACTGTAGAATTGCATTTGAGAAAAGGAGAAAATGGAGTTTAAGAAAATATTTTTCTTATTACTTTTGTATGAACTTCTTAGGCTCAGCTAATTGCACATTGCACAACTTGCCTTCAAAGATGTTGGATTCTGATAATGGTTTGGTGTGAAACTCATTGCAGTTGATGATGTGGTTCTTTATAAATTTGGAGAGAAATCTTCAGAGGCATTACGTTCAAGCGGATTTAAAGATGTTACATTCAAATCCTACAACAGGTATGTTACGGGAGGCCGTAAAGTTCTCAACCTTGTGTGTGTTTCCTTTCTGAATTGAGGAACTAAGTATCGTTATGGGTTCATGTTTCGTTTGCAGCCTTGGCCACTACACAGTGCCTGAAGAGATGGATGAGGTCTGTGCTTGGTTAACTTCAAAGTTGGGGCTATAGAGTGAGGGGAGGAGGCTCTTCAAATTGCAGAATTTTTAACCTTGCTGTTTAGACATTCTTTGACCTATAGTTGATTGGGAACAAATTTGGTGTGATATTATTATTCCTTTTAGACCTTATATTGGATATGGTTTTTACACCAAGATTGAATCCTTTTGGTGAACTGCAGAGATACCCTGACATGCAATTGGGATGACCCAATCATACACTTGATTGTTACTATTTATAATATCATTCCCTCTATGTTTTTGCTTGATGTTTTCATTTTGATATTCCTCCAGTTAAAAGGTTTGTCTACAAATTGAAATTGCTGTATTGGCAATCAAAATGATATTTGGTATGTCAGACTAAACCTTGCCTATCTTCAGAACATAAG

mRNA sequence

CACGTAGGCTGTTTGAGGGCCCCGATAAACCCAAAAGCAAGACTCATTTTATAGCAAATTCAAGCATATCTTGACACCAATTATTATAATTGAACTTCCTCCAGGTTGAATCGTTCATCGAAGATTAAAAAATCTCAACCTTTCCTAACTTTGGGAGTACCTATCGCTGCAGCCATGGCAAATTAAACACAACAGGTGCGAACCGGGCGGGGCGGGGACCAAACGCACACACCCCTCGAAACAAAATTGTGTGTGAGAGAGAGAGAGAAAGAGAGAGAAACAATCAATACAGCGACGACAGCAACAACGAAAACAAGATATAGCCCATAACCACACCCCACTTCAAACCCCTCTCTCTGGCTCCTCCCCCGCCCAGGATCAATTGATAGTCAATCTCTTAAGGTGGTGGTTTACTTGGCTTTTGTATTGCGAATATGAGTTTTGCTGGCTCTTCTGTTGCTGCTGGTGGTAGAGCTGCCAAAAAGGCAATCGAGTTTGGTAAAACCTACGTAGTGAGGCCGAAGGGAAAACACCAAGCTACTGTTGTCTGGCTTCATGGTCTTGGTGACAATGGCTCAAGCTGGTCGCAACTATTGGAGACGCTTCCTTTACCAAATATTAAATGGATATGTCCGACTGCGCCTACTCGACCAATAGCTTTATTTGGTGGCTTTCCATCCACTGCATGGTTTGATGTGGAAGATCTTTCAGAAGATGGTCCAGATGATTTGGAGGGTTTGGATGCTTCAGCCGCACATGTTGCATATTTGTTATCAACAGAGCCAGCTGACATTAAGCTTGGTGTAGGAGGGTTCAGTATGGGCGCAGCTACTGCGCTTTACTCCGCAACCTGCCACGCGGTGGGAAAATACGGGAACGGAAATCCTTATCCAGCCAATCTGAGTGCAGTTGTTGGGCTTAGTGGCTGGCTTCCATGTTCAAAGACTTTGAAAACCAATATGGAACAGAAGAACGCCGGTAATAGTGGAGCAGGATCCTTGCCTATTTTGCTATGCCATGGAAAAGTTGATGATGTGGTTCTTTATAAATTTGGAGAGAAATCTTCAGAGGCATTACGTTCAAGCGGATTTAAAGATGTTACATTCAAATCCTACAACAGCCTTGGCCACTACACAGTGCCTGAAGAGATGGATGAGGTCTGTGCTTGGTTAACTTCAAAGTTGGGGCTATAGAGTGAGGGGAGGAGGCTCTTCAAATTGCAGAATTTTTAACCTTGCTGTTTAGACATTCTTTGACCTATAGTTGATTGGGAACAAATTTGGTGTGATATTATTATTCCTTTTAGACCTTATATTGGATATGGTTTTTACACCAAGATTGAATCCTTTTGGTGAACTGCAGAGATACCCTGACATGCAATTGGGATGACCCAATCATACACTTGATTGTTACTATTTATAATATCATTCCCTCTATGTTTTTGCTTGATGTTTTCATTTTGATATTCCTCCAGTTAAAAGGTTTGTCTACAAATTGAAATTGCTGTATTGGCAATCAAAATGATATTTGGTATGTCAGACTAAACCTTGCCTATCTTCAGAACATAAG

Coding sequence (CDS)

ATGAGTTTTGCTGGCTCTTCTGTTGCTGCTGGTGGTAGAGCTGCCAAAAAGGCAATCGAGTTTGGTAAAACCTACGTAGTGAGGCCGAAGGGAAAACACCAAGCTACTGTTGTCTGGCTTCATGGTCTTGGTGACAATGGCTCAAGCTGGTCGCAACTATTGGAGACGCTTCCTTTACCAAATATTAAATGGATATGTCCGACTGCGCCTACTCGACCAATAGCTTTATTTGGTGGCTTTCCATCCACTGCATGGTTTGATGTGGAAGATCTTTCAGAAGATGGTCCAGATGATTTGGAGGGTTTGGATGCTTCAGCCGCACATGTTGCATATTTGTTATCAACAGAGCCAGCTGACATTAAGCTTGGTGTAGGAGGGTTCAGTATGGGCGCAGCTACTGCGCTTTACTCCGCAACCTGCCACGCGGTGGGAAAATACGGGAACGGAAATCCTTATCCAGCCAATCTGAGTGCAGTTGTTGGGCTTAGTGGCTGGCTTCCATGTTCAAAGACTTTGAAAACCAATATGGAACAGAAGAACGCCGGTAATAGTGGAGCAGGATCCTTGCCTATTTTGCTATGCCATGGAAAAGTTGATGATGTGGTTCTTTATAAATTTGGAGAGAAATCTTCAGAGGCATTACGTTCAAGCGGATTTAAAGATGTTACATTCAAATCCTACAACAGCCTTGGCCACTACACAGTGCCTGAAGAGATGGATGAGGTCTGTGCTTGGTTAACTTCAAAGTTGGGGCTATAG

Protein sequence

MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPADIKLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKNAGNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALRSSGFKDVTFKSYNSLGHYTVPEEMDEVCAWLTSKLGL*
Homology
BLAST of CSPI01G18550 vs. ExPASy Swiss-Prot
Match: O95372 (Acyl-protein thioesterase 2 OS=Homo sapiens OX=9606 GN=LYPLA2 PE=1 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 4.2e-32
Identity = 85/226 (37.61%), Postives = 123/226 (54.42%), Query Frame = 0

Query: 32  KHQATVVWLHGLGDNGSSWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDL 91
           +  A V++LHGLGD G SW+  L T+ LP++K+ICP AP  P+ L       +WFD+  L
Sbjct: 22  RETAAVIFLHGLGDTGHSWADALSTIRLPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGL 81

Query: 92  SEDGPDDLEGLDASAAHVAYLLSTE-----PADIKLGVGGFSMGAATALYSA-TCHAVGK 151
           S D P+D  G+  +A ++  L+  E     PA+ ++ +GGFS G A +LY+A TC     
Sbjct: 82  SPDAPEDEAGIKKAAENIKALIEHEMKNGIPAN-RIVLGGFSQGGALSLYTALTC----- 141

Query: 152 YGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKNAGNSGAGSLPILLCHGKVDDVVLYK 211
                P+P  L+ +V LS WLP  +          A N  A  L IL CHG++D +V  +
Sbjct: 142 -----PHP--LAGIVALSCWLPLHRAF------PQAANGSAKDLAILQCHGELDPMVPVR 201

Query: 212 FGEKSSEALRSSGF-KDVTFKSYNSLGHYTVPEEMDEVCAWLTSKL 251
           FG  ++E LRS      V FK+Y  + H + P+EM  V  +L   L
Sbjct: 202 FGALTAEKLRSVVTPARVQFKTYPGVMHSSCPQEMAAVKEFLEKLL 228

BLAST of CSPI01G18550 vs. ExPASy Swiss-Prot
Match: Q9WTL7 (Acyl-protein thioesterase 2 OS=Mus musculus OX=10090 GN=Lypla2 PE=1 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 4.2e-32
Identity = 87/226 (38.50%), Postives = 125/226 (55.31%), Query Frame = 0

Query: 32  KHQATVVWLHGLGDNGSSWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDL 91
           +  A V++LHGLGD G SW+  L T+ LP++K+ICP AP  P+ L       +WFD+  L
Sbjct: 22  RETAAVIFLHGLGDTGHSWADALSTIRLPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGL 81

Query: 92  SEDGPDDLEGLDASAAHVAYLLSTE-----PADIKLGVGGFSMGAATALYSA-TCHAVGK 151
           S D P+D  G+  +A ++  L+  E     PA+ ++ +GGFS G A +LY+A TC     
Sbjct: 82  SPDAPEDEAGIKKAAENIKALIEHEMKNGIPAN-RIVLGGFSQGGALSLYTALTC----- 141

Query: 152 YGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKNAGNSGAGSLPILLCHGKVDDVVLYK 211
                P+P  L+ +V LS WLP    L  N  Q  A N  A  L IL CHG++D +V  +
Sbjct: 142 -----PHP--LAGIVALSCWLP----LHRNFPQ--AANGSAKDLAILQCHGELDPMVPVR 201

Query: 212 FGEKSSEALRSSGF-KDVTFKSYNSLGHYTVPEEMDEVCAWLTSKL 251
           FG  ++E LR+      V FK+Y  + H + P+EM  V  +L   L
Sbjct: 202 FGALTAEKLRTVVTPARVQFKTYPGVMHSSCPQEMAAVKEFLEKLL 228

BLAST of CSPI01G18550 vs. ExPASy Swiss-Prot
Match: Q9QYL8 (Acyl-protein thioesterase 2 OS=Rattus norvegicus OX=10116 GN=Lypla2 PE=1 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 4.2e-32
Identity = 87/226 (38.50%), Postives = 125/226 (55.31%), Query Frame = 0

Query: 32  KHQATVVWLHGLGDNGSSWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDL 91
           +  A V++LHGLGD G SW+  L T+ LP++K+ICP AP  P+ L       +WFD+  L
Sbjct: 22  RETAAVIFLHGLGDTGHSWADALSTIRLPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGL 81

Query: 92  SEDGPDDLEGLDASAAHVAYLLSTE-----PADIKLGVGGFSMGAATALYSA-TCHAVGK 151
           S D P+D  G+  +A ++  L+  E     PA+ ++ +GGFS G A +LY+A TC     
Sbjct: 82  SPDAPEDEAGIKKAAENIKALIEHEMKNGIPAN-RIVLGGFSQGGALSLYTALTC----- 141

Query: 152 YGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKNAGNSGAGSLPILLCHGKVDDVVLYK 211
                P+P  L+ +V LS WLP    L  N  Q  A N  A  L IL CHG++D +V  +
Sbjct: 142 -----PHP--LAGIVALSCWLP----LHRNFPQ--AANGSAKDLAILQCHGELDPMVPVR 201

Query: 212 FGEKSSEALRSSGF-KDVTFKSYNSLGHYTVPEEMDEVCAWLTSKL 251
           FG  ++E LR+      V FK+Y  + H + P+EM  V  +L   L
Sbjct: 202 FGALTAEKLRTVVTPARVQFKTYPGVMHSSCPQEMAAVKEFLEKLL 228

BLAST of CSPI01G18550 vs. ExPASy Swiss-Prot
Match: Q4I8Q4 (Acyl-protein thioesterase 1 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) OX=229533 GN=FGRAMPH1_01T20223 PE=3 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 5.3e-27
Identity = 76/233 (32.62%), Postives = 120/233 (51.50%), Query Frame = 0

Query: 32  KHQATVVWLHGLGDNGSSWSQLLET----LPLPNIKWICPTAPTRPIALFGGFPSTAWFD 91
           +H ATV+++HGLGD G  W+  +E       +  +K+I P AP  PI++  G     WFD
Sbjct: 16  QHTATVIFVHGLGDTGHGWASAVENWRRRQKMDEVKFILPHAPQIPISVNMGMRMPGWFD 75

Query: 92  VEDLSED-----GPDDLEGLDASAAHVAYLLSTE----PADIKLGVGGFSMGAATALYSA 151
           ++ L  D       +D EG+  S  +   L+  E        ++ +GGFS G A +L + 
Sbjct: 76  IKQLGGDVDSLIRNEDTEGIKLSQKYFHNLIQQEIDSGIVPERIVLGGFSQGGAMSLLAG 135

Query: 152 -TCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKNAGNSGAGSLPILLCHGK 211
            TC             + L  ++GLS WL  SKT    ++  +A        P+++ HG+
Sbjct: 136 LTC------------TSKLGGILGLSSWLLLSKTFADMVKPTDANR----QTPVMMFHGE 195

Query: 212 VDDVVLYKFGEKSSEALRSSGFKDVTFKSYNSLGHYTVPEEMDEVCAWLTSKL 251
            D +V  + G+ S+E L+  G+ DV +K+Y  +GH  VPEE+DEV A+L  +L
Sbjct: 196 EDPIVPCERGKLSAELLKGLGY-DVAWKTYPGMGHSAVPEELDEVEAFLRKQL 231

BLAST of CSPI01G18550 vs. ExPASy Swiss-Prot
Match: P0CL95 (Acyl-protein thioesterase 1 OS=Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) OX=283643 GN=CNBF2260 PE=3 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 4.5e-26
Identity = 75/245 (30.61%), Postives = 120/245 (48.98%), Query Frame = 0

Query: 27  VRPKGKHQATVVWLHGLGDNGSSWSQLLETL--PLPNIKWICPTAPTRPIALFGGFPSTA 86
           + PK  H ATV++LHGLGD+G  W  + + L    PN+KWI P APT P++L  G    +
Sbjct: 10  ISPKEAHTATVIFLHGLGDSGHGWLPVAKMLWSSFPNVKWILPHAPTIPVSLNHGMAMPS 69

Query: 87  WFDV---EDLSEDGPDDLEGLDASAAHVAYLLSTEP----ADIKLGVGGFSMGAATALYS 146
           WFD+   + L     DD +G+  +   V  L+  E      + ++ +GGFS G A ++  
Sbjct: 70  WFDIRHLDKLDNSENDDEQGMLETLKSVDELIQAEVDSGIPENRIVLGGFSQGGAISVL- 129

Query: 147 ATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKNAGNSGAGSLPILLCHGK 206
                     N       L+ VV LS W+P +  +   M +       A  +P+   HG 
Sbjct: 130 ----------NMLTTKRKLAGVVALSTWVPLNHKIVQMMSEH------AKDIPVFWGHGT 189

Query: 207 VDDVVLYKFGEKSSEAL-RSSGFK-----------DVTFKSYNSLGHYTVPEEMDEVCAW 251
            D VV Y+FG++S + L +  G+K            + F+SY  + H + P+E++++ +W
Sbjct: 190 NDPVVDYRFGQRSVDFLVQKCGYKLLSQGTTFARPGIRFESYPGMPHSSCPQEIEDLKSW 237

BLAST of CSPI01G18550 vs. ExPASy TrEMBL
Match: A0A0A0LU24 (Abhydrolase_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G305760 PE=4 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 8.5e-145
Identity = 252/252 (100.00%), Postives = 252/252 (100.00%), Query Frame = 0

Query: 1   MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLP 60
           MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLP
Sbjct: 1   MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPADI 120
           NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPADI 120

Query: 121 KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKN 180
           KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKN
Sbjct: 121 KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKN 180

Query: 181 AGNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALRSSGFKDVTFKSYNSLGHYTVPEEMD 240
           AGNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALRSSGFKDVTFKSYNSLGHYTVPEEMD
Sbjct: 181 AGNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALRSSGFKDVTFKSYNSLGHYTVPEEMD 240

Query: 241 EVCAWLTSKLGL 253
           EVCAWLTSKLGL
Sbjct: 241 EVCAWLTSKLGL 252

BLAST of CSPI01G18550 vs. ExPASy TrEMBL
Match: A0A1S3CMW5 (acyl-protein thioesterase 2 OS=Cucumis melo OX=3656 GN=LOC103502735 PE=4 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 1.1e-139
Identity = 244/252 (96.83%), Postives = 247/252 (98.02%), Query Frame = 0

Query: 1   MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLP 60
           MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLP
Sbjct: 1   MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPADI 120
           NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSE+GPDDLEGLDASAAHVAYLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSENGPDDLEGLDASAAHVAYLLSTEPADI 120

Query: 121 KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKN 180
           KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKT ME+K 
Sbjct: 121 KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTKMEEKK 180

Query: 181 AGNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALRSSGFKDVTFKSYNSLGHYTVPEEMD 240
           A N GAGSLPILLCHGKVDDVVLYKFGEKSSEAL SSGFKDVTFKSY+SLGHYTVPEEMD
Sbjct: 181 ASNIGAGSLPILLCHGKVDDVVLYKFGEKSSEALLSSGFKDVTFKSYSSLGHYTVPEEMD 240

Query: 241 EVCAWLTSKLGL 253
           EVCAWLTSKLGL
Sbjct: 241 EVCAWLTSKLGL 252

BLAST of CSPI01G18550 vs. ExPASy TrEMBL
Match: A0A6J1JJZ2 (acyl-protein thioesterase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111485265 PE=4 SV=1)

HSP 1 Score: 490.7 bits (1262), Expect = 3.6e-135
Identity = 232/252 (92.06%), Postives = 243/252 (96.43%), Query Frame = 0

Query: 1   MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLP 60
           MS AGSSVA+GG+AA++AIEFGKTYVVRPKGKHQAT+VWLHGLGDNGSSWSQLLE LPLP
Sbjct: 1   MSLAGSSVASGGKAARRAIEFGKTYVVRPKGKHQATIVWLHGLGDNGSSWSQLLEMLPLP 60

Query: 61  NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPADI 120
           NIKWICPTAPTRPIALFGGFPSTAWFDV+DLSEDGPDDLEGLDASAAHVAYLLSTEP+DI
Sbjct: 61  NIKWICPTAPTRPIALFGGFPSTAWFDVKDLSEDGPDDLEGLDASAAHVAYLLSTEPSDI 120

Query: 121 KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKN 180
           KLGVGGFSMGAATALYSATCHAVGKYGNGNPYP NLSAVVGLSGWLPCSKTLK+ ME+KN
Sbjct: 121 KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPTNLSAVVGLSGWLPCSKTLKSKMEEKN 180

Query: 181 AGNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALRSSGFKDVTFKSYNSLGHYTVPEEMD 240
           A NSGAGSLPILLCHGKVDDVVLYKFGEKSS+AL S GF+DV FKSYN LGHYTVPEEMD
Sbjct: 181 ASNSGAGSLPILLCHGKVDDVVLYKFGEKSSQALHSVGFQDVIFKSYNGLGHYTVPEEMD 240

Query: 241 EVCAWLTSKLGL 253
           EVCAWLTSKLGL
Sbjct: 241 EVCAWLTSKLGL 252

BLAST of CSPI01G18550 vs. ExPASy TrEMBL
Match: A0A6J1H9A6 (acyl-protein thioesterase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111461683 PE=4 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 3.0e-134
Identity = 231/252 (91.67%), Postives = 242/252 (96.03%), Query Frame = 0

Query: 1   MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLP 60
           MS AGSSVA+GG+AA++AIEFGKTYVVRPKGKHQAT+VWLHGLGDNGSSWSQLLE LPLP
Sbjct: 1   MSLAGSSVASGGKAARRAIEFGKTYVVRPKGKHQATIVWLHGLGDNGSSWSQLLEMLPLP 60

Query: 61  NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPADI 120
           NIKWICPTAPTR IALFGGFPSTAWFDV+DLSEDGPDDLEGLDASAAHVAYLLSTEP+DI
Sbjct: 61  NIKWICPTAPTRSIALFGGFPSTAWFDVKDLSEDGPDDLEGLDASAAHVAYLLSTEPSDI 120

Query: 121 KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKN 180
           KLGVGGFSMGAATALYSATCHAVGKYGNGNPYP NLSAVVGLSGWLPCSKTLK+ ME+KN
Sbjct: 121 KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPTNLSAVVGLSGWLPCSKTLKSKMEEKN 180

Query: 181 AGNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALRSSGFKDVTFKSYNSLGHYTVPEEMD 240
           A NSGAGSLPILLCHGKVDDVVLYKFGEKSS+AL S GF+DV FKSYN LGHYTVPEEMD
Sbjct: 181 ASNSGAGSLPILLCHGKVDDVVLYKFGEKSSQALHSVGFQDVIFKSYNGLGHYTVPEEMD 240

Query: 241 EVCAWLTSKLGL 253
           EVCAWLTSKLGL
Sbjct: 241 EVCAWLTSKLGL 252

BLAST of CSPI01G18550 vs. ExPASy TrEMBL
Match: A0A6J1EBV8 (acyl-protein thioesterase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111432778 PE=4 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 2.6e-133
Identity = 228/252 (90.48%), Postives = 243/252 (96.43%), Query Frame = 0

Query: 1   MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLP 60
           MSFAGSSV +G + A+KAIEFGKT+VVRPKG+HQATVVWLHGLGDNG+SWSQLLETLPLP
Sbjct: 1   MSFAGSSVVSGVKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPADI 120
           NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEP+DI
Sbjct: 61  NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDI 120

Query: 121 KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKN 180
           KL VGGFSMGAATALYSATCH++GKYGNGNPYPANLSAVVGLSGWLPCSKTLK+ ME+  
Sbjct: 121 KLAVGGFSMGAATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMK 180

Query: 181 AGNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALRSSGFKDVTFKSYNSLGHYTVPEEMD 240
           A NSGAGSLP+LLCHGKVDDVVLYKFGEKSS+ALRS GF+DVTFKSYN+LGHYTVPEEMD
Sbjct: 181 ASNSGAGSLPVLLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMD 240

Query: 241 EVCAWLTSKLGL 253
           EVCAWLTSKLGL
Sbjct: 241 EVCAWLTSKLGL 252

BLAST of CSPI01G18550 vs. NCBI nr
Match: XP_004150012.1 (acyl-protein thioesterase 2 [Cucumis sativus] >XP_031736139.1 acyl-protein thioesterase 2 [Cucumis sativus] >KGN65303.1 hypothetical protein Csa_019754 [Cucumis sativus])

HSP 1 Score: 522.7 bits (1345), Expect = 1.8e-144
Identity = 252/252 (100.00%), Postives = 252/252 (100.00%), Query Frame = 0

Query: 1   MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLP 60
           MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLP
Sbjct: 1   MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPADI 120
           NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPADI 120

Query: 121 KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKN 180
           KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKN
Sbjct: 121 KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKN 180

Query: 181 AGNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALRSSGFKDVTFKSYNSLGHYTVPEEMD 240
           AGNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALRSSGFKDVTFKSYNSLGHYTVPEEMD
Sbjct: 181 AGNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALRSSGFKDVTFKSYNSLGHYTVPEEMD 240

Query: 241 EVCAWLTSKLGL 253
           EVCAWLTSKLGL
Sbjct: 241 EVCAWLTSKLGL 252

BLAST of CSPI01G18550 vs. NCBI nr
Match: XP_038879397.1 (acyl-protein thioesterase 2 [Benincasa hispida])

HSP 1 Score: 506.9 bits (1304), Expect = 1.0e-139
Identity = 243/252 (96.43%), Postives = 246/252 (97.62%), Query Frame = 0

Query: 1   MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLP 60
           MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLP
Sbjct: 1   MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPADI 120
           NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPADI 120

Query: 121 KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKN 180
           KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKT ME+KN
Sbjct: 121 KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTKMEEKN 180

Query: 181 AGNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALRSSGFKDVTFKSYNSLGHYTVPEEMD 240
           A NSG GSLPILLCHGKVDDVVLYKFGEKSSEAL S GFKDVTFKSY+ +GHYTVPEEMD
Sbjct: 181 ANNSGVGSLPILLCHGKVDDVVLYKFGEKSSEALHSCGFKDVTFKSYSGVGHYTVPEEMD 240

Query: 241 EVCAWLTSKLGL 253
           EVCAWLTSKLGL
Sbjct: 241 EVCAWLTSKLGL 252

BLAST of CSPI01G18550 vs. NCBI nr
Match: XP_008465012.1 (PREDICTED: acyl-protein thioesterase 2 [Cucumis melo] >XP_008465013.1 PREDICTED: acyl-protein thioesterase 2 [Cucumis melo])

HSP 1 Score: 505.8 bits (1301), Expect = 2.2e-139
Identity = 244/252 (96.83%), Postives = 247/252 (98.02%), Query Frame = 0

Query: 1   MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLP 60
           MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLP
Sbjct: 1   MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPADI 120
           NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSE+GPDDLEGLDASAAHVAYLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSENGPDDLEGLDASAAHVAYLLSTEPADI 120

Query: 121 KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKN 180
           KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKT ME+K 
Sbjct: 121 KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTKMEEKK 180

Query: 181 AGNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALRSSGFKDVTFKSYNSLGHYTVPEEMD 240
           A N GAGSLPILLCHGKVDDVVLYKFGEKSSEAL SSGFKDVTFKSY+SLGHYTVPEEMD
Sbjct: 181 ASNIGAGSLPILLCHGKVDDVVLYKFGEKSSEALLSSGFKDVTFKSYSSLGHYTVPEEMD 240

Query: 241 EVCAWLTSKLGL 253
           EVCAWLTSKLGL
Sbjct: 241 EVCAWLTSKLGL 252

BLAST of CSPI01G18550 vs. NCBI nr
Match: XP_022987844.1 (acyl-protein thioesterase 2-like [Cucurbita maxima] >XP_022987845.1 acyl-protein thioesterase 2-like [Cucurbita maxima])

HSP 1 Score: 490.7 bits (1262), Expect = 7.4e-135
Identity = 232/252 (92.06%), Postives = 243/252 (96.43%), Query Frame = 0

Query: 1   MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLP 60
           MS AGSSVA+GG+AA++AIEFGKTYVVRPKGKHQAT+VWLHGLGDNGSSWSQLLE LPLP
Sbjct: 1   MSLAGSSVASGGKAARRAIEFGKTYVVRPKGKHQATIVWLHGLGDNGSSWSQLLEMLPLP 60

Query: 61  NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPADI 120
           NIKWICPTAPTRPIALFGGFPSTAWFDV+DLSEDGPDDLEGLDASAAHVAYLLSTEP+DI
Sbjct: 61  NIKWICPTAPTRPIALFGGFPSTAWFDVKDLSEDGPDDLEGLDASAAHVAYLLSTEPSDI 120

Query: 121 KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKN 180
           KLGVGGFSMGAATALYSATCHAVGKYGNGNPYP NLSAVVGLSGWLPCSKTLK+ ME+KN
Sbjct: 121 KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPTNLSAVVGLSGWLPCSKTLKSKMEEKN 180

Query: 181 AGNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALRSSGFKDVTFKSYNSLGHYTVPEEMD 240
           A NSGAGSLPILLCHGKVDDVVLYKFGEKSS+AL S GF+DV FKSYN LGHYTVPEEMD
Sbjct: 181 ASNSGAGSLPILLCHGKVDDVVLYKFGEKSSQALHSVGFQDVIFKSYNGLGHYTVPEEMD 240

Query: 241 EVCAWLTSKLGL 253
           EVCAWLTSKLGL
Sbjct: 241 EVCAWLTSKLGL 252

BLAST of CSPI01G18550 vs. NCBI nr
Match: XP_023515795.1 (acyl-protein thioesterase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 489.2 bits (1258), Expect = 2.2e-134
Identity = 232/252 (92.06%), Postives = 242/252 (96.03%), Query Frame = 0

Query: 1   MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLP 60
           MS AGSSVA+GG+AA++AIEFGKTYVVRPKGKHQAT+VWLHGLGDNGSSWSQLLE LPLP
Sbjct: 1   MSLAGSSVASGGKAARRAIEFGKTYVVRPKGKHQATIVWLHGLGDNGSSWSQLLEMLPLP 60

Query: 61  NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPADI 120
           NIKWICPTAPTRPIALFGGFPSTAWFDV+DLSE GPDDLEGLDASAAHVAYLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPIALFGGFPSTAWFDVKDLSEGGPDDLEGLDASAAHVAYLLSTEPADI 120

Query: 121 KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKN 180
           KLGVGGFSMGAATALYSATCHAVGKYGNGNPYP NLSAVVGLSGWLPCSKTLK+ ME+KN
Sbjct: 121 KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPTNLSAVVGLSGWLPCSKTLKSKMEEKN 180

Query: 181 AGNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALRSSGFKDVTFKSYNSLGHYTVPEEMD 240
           A NSGAGSLPILLCHGKVDDVVLYKFGEKSS+AL S GF+DV FKSYN LGHYTVPEEMD
Sbjct: 181 ASNSGAGSLPILLCHGKVDDVVLYKFGEKSSQALHSVGFQDVIFKSYNGLGHYTVPEEMD 240

Query: 241 EVCAWLTSKLGL 253
           EVCAWLTSKLGL
Sbjct: 241 EVCAWLTSKLGL 252

BLAST of CSPI01G18550 vs. TAIR 10
Match: AT5G20060.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 410.2 bits (1053), Expect = 1.2e-114
Identity = 189/252 (75.00%), Postives = 225/252 (89.29%), Query Frame = 0

Query: 1   MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLP 60
           MS +G++V + GR  ++A+EFGKT+VVRPKGKHQAT+VWLHGLGDNGSSWSQLLETLPLP
Sbjct: 1   MSISGAAVGS-GRNLRRAVEFGKTHVVRPKGKHQATIVWLHGLGDNGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPADI 120
           NIKWICPTAP++PI+LFGGFPSTAWFDV D++EDGPDD+EGLD +AAHVA LLS EPADI
Sbjct: 61  NIKWICPTAPSQPISLFGGFPSTAWFDVVDINEDGPDDMEGLDVAAAHVANLLSNEPADI 120

Query: 121 KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKN 180
           KLGVGGFSMGAAT+LYSATC A+GKYGNGNPYP NLSA++GLSGWLPC+KTL   +E++ 
Sbjct: 121 KLGVGGFSMGAATSLYSATCFALGKYGNGNPYPINLSAIIGLSGWLPCAKTLAGKLEEEQ 180

Query: 181 AGNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALRSSGFKDVTFKSYNSLGHYTVPEEMD 240
             N  A SLPI++CHGK DDVV +KFGEKSS+AL S+GFK VTFK Y++LGH+T+P+E+D
Sbjct: 181 IKNR-AASLPIVVCHGKADDVVPFKFGEKSSQALLSNGFKKVTFKPYSALGHHTIPQELD 240

Query: 241 EVCAWLTSKLGL 253
           E+CAWLTS L L
Sbjct: 241 ELCAWLTSTLSL 250

BLAST of CSPI01G18550 vs. TAIR 10
Match: AT5G20060.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 410.2 bits (1053), Expect = 1.2e-114
Identity = 189/252 (75.00%), Postives = 225/252 (89.29%), Query Frame = 0

Query: 1   MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLP 60
           MS +G++V + GR  ++A+EFGKT+VVRPKGKHQAT+VWLHGLGDNGSSWSQLLETLPLP
Sbjct: 1   MSISGAAVGS-GRNLRRAVEFGKTHVVRPKGKHQATIVWLHGLGDNGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPADI 120
           NIKWICPTAP++PI+LFGGFPSTAWFDV D++EDGPDD+EGLD +AAHVA LLS EPADI
Sbjct: 61  NIKWICPTAPSQPISLFGGFPSTAWFDVVDINEDGPDDMEGLDVAAAHVANLLSNEPADI 120

Query: 121 KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKN 180
           KLGVGGFSMGAAT+LYSATC A+GKYGNGNPYP NLSA++GLSGWLPC+KTL   +E++ 
Sbjct: 121 KLGVGGFSMGAATSLYSATCFALGKYGNGNPYPINLSAIIGLSGWLPCAKTLAGKLEEEQ 180

Query: 181 AGNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALRSSGFKDVTFKSYNSLGHYTVPEEMD 240
             N  A SLPI++CHGK DDVV +KFGEKSS+AL S+GFK VTFK Y++LGH+T+P+E+D
Sbjct: 181 IKNR-AASLPIVVCHGKADDVVPFKFGEKSSQALLSNGFKKVTFKPYSALGHHTIPQELD 240

Query: 241 EVCAWLTSKLGL 253
           E+CAWLTS L L
Sbjct: 241 ELCAWLTSTLSL 250

BLAST of CSPI01G18550 vs. TAIR 10
Match: AT5G20060.3 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 410.2 bits (1053), Expect = 1.2e-114
Identity = 189/252 (75.00%), Postives = 225/252 (89.29%), Query Frame = 0

Query: 1   MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLP 60
           MS +G++V + GR  ++A+EFGKT+VVRPKGKHQAT+VWLHGLGDNGSSWSQLLETLPLP
Sbjct: 1   MSISGAAVGS-GRNLRRAVEFGKTHVVRPKGKHQATIVWLHGLGDNGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPADI 120
           NIKWICPTAP++PI+LFGGFPSTAWFDV D++EDGPDD+EGLD +AAHVA LLS EPADI
Sbjct: 61  NIKWICPTAPSQPISLFGGFPSTAWFDVVDINEDGPDDMEGLDVAAAHVANLLSNEPADI 120

Query: 121 KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKN 180
           KLGVGGFSMGAAT+LYSATC A+GKYGNGNPYP NLSA++GLSGWLPC+KTL   +E++ 
Sbjct: 121 KLGVGGFSMGAATSLYSATCFALGKYGNGNPYPINLSAIIGLSGWLPCAKTLAGKLEEEQ 180

Query: 181 AGNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALRSSGFKDVTFKSYNSLGHYTVPEEMD 240
             N  A SLPI++CHGK DDVV +KFGEKSS+AL S+GFK VTFK Y++LGH+T+P+E+D
Sbjct: 181 IKNR-AASLPIVVCHGKADDVVPFKFGEKSSQALLSNGFKKVTFKPYSALGHHTIPQELD 240

Query: 241 EVCAWLTSKLGL 253
           E+CAWLTS L L
Sbjct: 241 ELCAWLTSTLSL 250

BLAST of CSPI01G18550 vs. TAIR 10
Match: AT3G15650.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 350.9 bits (899), Expect = 8.5e-97
Identity = 164/252 (65.08%), Postives = 200/252 (79.37%), Query Frame = 0

Query: 1   MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLP 60
           MS++  S+ +G R+  +  EFG+TYVVRPKGKHQAT+VWLHGLGDNGSS SQLLE+LPLP
Sbjct: 1   MSYSRQSMGSGSRST-RGYEFGRTYVVRPKGKHQATIVWLHGLGDNGSSSSQLLESLPLP 60

Query: 61  NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPADI 120
           NIKWICPTAP+RP++L GGFP TAWFDV ++SED  DD+EGLDASAAH+A LLS EP D+
Sbjct: 61  NIKWICPTAPSRPVSLLGGFPCTAWFDVGEISEDLHDDIEGLDASAAHIANLLSAEPTDV 120

Query: 121 KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKN 180
           K+G+GGFSMGAA ALYS TC+A+G+YG G+ Y  NL A VGLSGWLP  ++L++ +E  N
Sbjct: 121 KVGIGGFSMGAAIALYSTTCYALGRYGTGHAYTINLRATVGLSGWLPGWRSLRSKIESSN 180

Query: 181 AGNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALRSSGFKDVTFKSYNSLGHYTVPEEMD 240
                A S+PILL HG  DDVV Y+FGEKS+ +L  +GF+   FK Y  LGHYTVP+EMD
Sbjct: 181 EVARRAASIPILLAHGTSDDVVPYRFGEKSAHSLAMAGFRQTMFKPYEGLGHYTVPKEMD 240

Query: 241 EVCAWLTSKLGL 253
           EV  WL S+LGL
Sbjct: 241 EVVHWLVSRLGL 251

BLAST of CSPI01G18550 vs. TAIR 10
Match: AT1G52700.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 345.5 bits (885), Expect = 3.6e-95
Identity = 164/252 (65.08%), Postives = 201/252 (79.76%), Query Frame = 0

Query: 1   MSFAGSSVAAGGRAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLP 60
           MS++  S+ +G R A +  EFG+TYVVRPKGKHQAT+VWLHGLGDNGSS SQL+++L LP
Sbjct: 1   MSYSHQSMGSGSRNA-RGYEFGRTYVVRPKGKHQATLVWLHGLGDNGSSSSQLMDSLHLP 60

Query: 61  NIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPADI 120
           NIKWICPTAP+RP+   GGF  TAWFDV ++SEDG DDLEGLDASA+H+A LLS+EPAD+
Sbjct: 61  NIKWICPTAPSRPVTSLGGFTCTAWFDVGEISEDGHDDLEGLDASASHIANLLSSEPADV 120

Query: 121 KLGVGGFSMGAATALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKN 180
           K+G+GGFSMGAA +LYSATC+A+G+YG G+ YP NL AVVGLSGWLP  K+L++ +E   
Sbjct: 121 KVGIGGFSMGAAISLYSATCYALGRYGTGHAYPINLQAVVGLSGWLPGWKSLRSKIECSF 180

Query: 181 AGNSGAGSLPILLCHGKVDDVVLYKFGEKSSEALRSSGFKDVTFKSYNSLGHYTVPEEMD 240
                A SLPI+L HG  DDVV Y+FGEKS+++L  +GF+   FK Y  LGHYTVP EMD
Sbjct: 181 EAARRAASLPIILTHGTSDDVVPYRFGEKSAQSLGMAGFRLAMFKPYEGLGHYTVPREMD 240

Query: 241 EVCAWLTSKLGL 253
           EV  WLT+ LGL
Sbjct: 241 EVVHWLTTMLGL 251

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O953724.2e-3237.61Acyl-protein thioesterase 2 OS=Homo sapiens OX=9606 GN=LYPLA2 PE=1 SV=1[more]
Q9WTL74.2e-3238.50Acyl-protein thioesterase 2 OS=Mus musculus OX=10090 GN=Lypla2 PE=1 SV=1[more]
Q9QYL84.2e-3238.50Acyl-protein thioesterase 2 OS=Rattus norvegicus OX=10116 GN=Lypla2 PE=1 SV=1[more]
Q4I8Q45.3e-2732.62Acyl-protein thioesterase 1 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 12365... [more]
P0CL954.5e-2630.61Acyl-protein thioesterase 1 OS=Cryptococcus neoformans var. neoformans serotype ... [more]
Match NameE-valueIdentityDescription
A0A0A0LU248.5e-145100.00Abhydrolase_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G3057... [more]
A0A1S3CMW51.1e-13996.83acyl-protein thioesterase 2 OS=Cucumis melo OX=3656 GN=LOC103502735 PE=4 SV=1[more]
A0A6J1JJZ23.6e-13592.06acyl-protein thioesterase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111485265 PE=... [more]
A0A6J1H9A63.0e-13491.67acyl-protein thioesterase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111461683 P... [more]
A0A6J1EBV82.6e-13390.48acyl-protein thioesterase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111432778 P... [more]
Match NameE-valueIdentityDescription
XP_004150012.11.8e-144100.00acyl-protein thioesterase 2 [Cucumis sativus] >XP_031736139.1 acyl-protein thioe... [more]
XP_038879397.11.0e-13996.43acyl-protein thioesterase 2 [Benincasa hispida][more]
XP_008465012.12.2e-13996.83PREDICTED: acyl-protein thioesterase 2 [Cucumis melo] >XP_008465013.1 PREDICTED:... [more]
XP_022987844.17.4e-13592.06acyl-protein thioesterase 2-like [Cucurbita maxima] >XP_022987845.1 acyl-protein... [more]
XP_023515795.12.2e-13492.06acyl-protein thioesterase 2-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G20060.21.2e-11475.00alpha/beta-Hydrolases superfamily protein [more]
AT5G20060.11.2e-11475.00alpha/beta-Hydrolases superfamily protein [more]
AT5G20060.31.2e-11475.00alpha/beta-Hydrolases superfamily protein [more]
AT3G15650.18.5e-9765.08alpha/beta-Hydrolases superfamily protein [more]
AT1G52700.13.6e-9565.08alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 10..247
e-value: 9.2E-64
score: 217.1
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 21..246
IPR003140Phospholipase/carboxylesterase/thioesterasePFAMPF02230Abhydrolase_2coord: 21..242
e-value: 3.2E-39
score: 135.0
NoneNo IPR availablePANTHERPTHR46234:SF9ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 6..248
NoneNo IPR availablePANTHERPTHR46234ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 6..248

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G18550.1CSPI01G18550.1mRNA
CSPI01G18550.2CSPI01G18550.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity