CSPI01G17050 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G17050
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Chr1: 12847722 .. 12849615 (+)
RNA-Seq Expression	CSPI01G17050
Synteny	CSPI01G17050

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCACAAAATTCTCAGAACACACAAACACAAACACTCCAACAACAACTTCTCATCAACGGCGGCCACACGCCGGAAAGTTATATTTACAAAGGCGGCTACCACGGCGGAGGTTCCAACAATAATACTCCACTTCCACTGGCAGAGATTCCGGTCGTTGACCTCTCTCAACTTTCATTGCCGTCTGCCGGCGAGGGTCCGTTGAACGACCTCCGTTTGGCTCTTAGTACTTGGGGATGCTTTCAGGTGAGCTTTCATGAACCTCAACACTGCCCTTTTCATATCAACTTTATTTTTAATTCTATTTTCTTCTAATTTTCTGCTTCAAACTTTTAATTTTACATTTCGATTTTTTTTAAAAATATATTTGAGTTTTTAGCTAAATTTTGAGAGGAAAGATTAGTAAATGGAGACAATTATTTTTAAGTTTCAAAACTTATTATTAATATTATGTTTGTGGTTTTTAAAAGATGGGATAATATAAAGAAATACATTATTAGATATACTTTTGTTTATTTGCTTGTGTCCAACATTATTGATGACACATATCTGTTTGTTTTTTTGTATGTTAATTCTATATATTTTTAGACAAAAGTATTGAATGATCAACAATATACAATTGCATAACACTTTTTTCATATTATAAACGTATGACTAGTATAGAGGGTAATCAGATATTAATTTACTTTTACATTTTTTATTTTTACAATTAAAAAAATATAGTGACATATACTCTATTATTATAAATGTTTTTTTTGCAAACTCCCTACTTTTTTGGTTGTATTTACAAATCGATTATGGTACAATTAGTCTTACCTATACTTATGTAACATCAAGAATATATATTTATATATATATATATATATGGAATGTTTGGCAACTAATTATATCTCCATTTTCTAACCATTTGGATTTTTTATTTTTATTTTTACAATTAAATCATTTTCTTGTAATTATTTAAATCTTTCTTGAATTTTTAGCAAAATTCAAAAATAAAATAATATTGACAATAATAAGGAATCCTTTGGTTTTCAAATTTTTATTTGATTTTTTAAAAATATTAATTTAAAACGTAGATTACAGTACAAACTATTTAAACATGGAAATAGTATTCATGTGCTTAATTAAAAATTAAAAATTAAAAAAAAAAAAACTTAAAAAGAAAAACCAACTATCCCAATATTTCCAAAAATGAGCTCTAATTCGAAATAGTAAGTTATATTAAGAATTCCTTTTTTTTTTCAAGAAATGAGAAACCATAGTATGTAGATATGAATTATAGTTTTTTCTTAAAAAATTGAAATAAAATAGTAATGGCTAAATGGTCATGAAACGGAGCAAAAGGGAAAGAGGAGTGATTGTTGTAAATTAATTTAGATTAACTAATTTGAGAGTGTGGTTTCAGGCTATTAATCACAGCATTTCAAGTTCATTTTTGGAGAAGATGAGAAAAATAAGTGAGCAATTTTTCTCATTGCCCATTGAAGAGAAGATGAGATATGGAAGAGAAGTTGATGGAATGGAAGGATATGGGAATGATTTAATTCTTTCAGAACAACAAATTCTAGATTGGTCTGATCGTTTATATTTCGTGACAAATCCTGAAGATGAAAGACGTCTCGAGCTTTGGCCTTTAAATCCTCCATCTTTCAGGTTAGTTTCAACGCTAATCTTTCATATATCTAAATCAGTTCAACATTCTCAAAAATTGTCACTACAAGAATGAAAACCTTTCTCGACGCAAAAATACGTCTAAAAAAACACAAAATGCGTCGGAGAAAGTATTTCCAACATCCATATGCAACGTCGGCTTGAACGTCGGAGAAACAACGTTGGGGACACCTTCCCCGGCACGTGCGACGCGAACGCCAGTGTAATTTTTACCGATGCATAA

mRNA sequence

ATGGCACAAAATTCTCAGAACACACAAACACAAACACTCCAACAACAACTTCTCATCAACGGCGGCCACACGCCGGAAAGTTATATTTACAAAGGCGGCTACCACGGCGGAGGTTCCAACAATAATACTCCACTTCCACTGGCAGAGATTCCGGTCGTTGACCTCTCTCAACTTTCATTGCCGTCTGCCGGCGAGGGTCCGTTGAACGACCTCCGTTTGGCTCTTAGTACTTGGGGATGCTTTCAGGCTATTAATCACAGCATTTCAAGTTCATTTTTGGAGAAGATGAGAAAAATAAGTGAGCAATTTTTCTCATTGCCCATTGAAGAGAAGATGAGATATGGAAGAGAAGTTGATGGAATGGAAGGATATGGGAATGATTTAATTCTTTCAGAACAACAAATTCTAGATTGGTCTGATCGTTTATATTTCGTGACAAATCCTGAAGATGAAAGACGTCTCGAGCTTTGGCCTTTAAATCCTCCATCTTTCAGAAACAACGTTGGGGACACCTTCCCCGGCACGTGCGACGCGAACGCCAGTGTAATTTTTACCGATGCATAA

Coding sequence (CDS)

ATGGCACAAAATTCTCAGAACACACAAACACAAACACTCCAACAACAACTTCTCATCAACGGCGGCCACACGCCGGAAAGTTATATTTACAAAGGCGGCTACCACGGCGGAGGTTCCAACAATAATACTCCACTTCCACTGGCAGAGATTCCGGTCGTTGACCTCTCTCAACTTTCATTGCCGTCTGCCGGCGAGGGTCCGTTGAACGACCTCCGTTTGGCTCTTAGTACTTGGGGATGCTTTCAGGCTATTAATCACAGCATTTCAAGTTCATTTTTGGAGAAGATGAGAAAAATAAGTGAGCAATTTTTCTCATTGCCCATTGAAGAGAAGATGAGATATGGAAGAGAAGTTGATGGAATGGAAGGATATGGGAATGATTTAATTCTTTCAGAACAACAAATTCTAGATTGGTCTGATCGTTTATATTTCGTGACAAATCCTGAAGATGAAAGACGTCTCGAGCTTTGGCCTTTAAATCCTCCATCTTTCAGAAACAACGTTGGGGACACCTTCCCCGGCACGTGCGACGCGAACGCCAGTGTAATTTTTACCGATGCATAA

Protein sequence

MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSLPSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDGMEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSFRNNVGDTFPGTCDANASVIFTDA*

Homology

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BLAST of CSPI01G17050 vs. ExPASy Swiss-Prot
Match: A2A1A0 (S-norcoclaurine synthase 1 OS=Coptis japonica OX=3442 GN=NCS1 PE=1 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.7e-22
Identity = 51/120 (42.50%), Postives = 68/120 (56.67%), Query Frame = 0


Query: 49  EIPVVDLSQLSLPSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPI 108
           EIPV+DLS+L         L     A   WG FQ INH +    +EKM+  +E FF LP 
Sbjct: 50  EIPVIDLSRLLDQQYACDELAKFHSACLDWGFFQLINHGVREEVIEKMKVDTEDFFRLPF 109

Query: 109 EEKMRYGREVDGMEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSFRNNV 168
           +EK  Y +  +GMEGYG   + SE+Q LDW+D  + +T P  ER +  WP +P SFR  +
Sbjct: 110 KEKNAYRQLPNGMEGYGQAFVTSEEQKLDWADMHFLITKPVQERNMRFWPTSPTSFRETM 169

[Close]

BLAST of CSPI01G17050 vs. ExPASy Swiss-Prot
Match: Q94LP4 (2-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD11 PE=1 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 9.4e-21
Identity = 53/146 (36.30%), Postives = 75/146 (51.37%), Query Frame = 0


Query: 23  HTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSLPSAGEGPLNDLRLALSTWGCFQ 82
           H PE YI +         NN    +A IP++DL +L  P + E     LR A   WG F 
Sbjct: 28  HIPERYI-RPEASSEEVINNYQGDMA-IPIIDLKKLLCPQSSEEECVKLRSACQYWGFFL 87

Query: 83  AINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDGMEGYGNDLILSEQQILDWSDRL 142
            INH +    +  +++    FFS P++ K  Y +  + +EGYG   + SE Q LDW+D L
Sbjct: 88  LINHGVPDEVIANLKRDIVDFFSQPLDTKKEYTQLPNSLEGYGQSFVFSEDQKLDWADML 147

Query: 143 YFVTNPEDERRLELWPLNPPSFRNNV 169
           Y   +P D R L  WP +P SFR ++
Sbjct: 148 YLHVHPSDSRDLRFWPTSPASFRQSI 171

[Close]

BLAST of CSPI01G17050 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 2.7e-20
Identity = 44/120 (36.67%), Postives = 72/120 (60.00%), Query Frame = 0


Query: 49  EIPVVDLSQLSLPSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPI 108
           EIP++D+ +L   +  +  +  L  A   WG FQ +NH I SSFL+K++   + FF+LP+
Sbjct: 52  EIPIIDMKRLCSSTTMDSEVEKLDFACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPM 111

Query: 109 EEKMRYGREVDGMEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSFRNNV 168
           EEK ++ +  D +EG+G   ++SE Q LDW+D  +    P + R+  L+P  P  FR+ +
Sbjct: 112 EEKKKFWQRPDEIEGFGQAFVVSEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTL 171

[Close]

BLAST of CSPI01G17050 vs. ExPASy Swiss-Prot
Match: O80449 (Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 2.4e-16
Identity = 44/117 (37.61%), Postives = 62/117 (52.99%), Query Frame = 0


Query: 49  EIPVVDLSQLSLPSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPI 108
           EIPV+D++ +     G   L  +R A   WG FQ +NH ++ S +E++R    +FF LP+
Sbjct: 47  EIPVLDMNDVWGKPEG---LRLVRSACEEWGFFQMVNHGVTHSLMERVRGAWREFFELPL 106

Query: 109 EEKMRYGREVDGMEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSFR 166
           EEK +Y    D  EGYG+ L + +   LDWSD  +    P   R    WP  PP  R
Sbjct: 107 EEKRKYANSPDTYEGYGSRLGVVKDAKLDWSDYFFLNYLPSSIRNPSKWPSQPPKIR 160

[Close]

BLAST of CSPI01G17050 vs. ExPASy Swiss-Prot
Match: A0A4D6Q440 (Flavonol synthase 1 OS=Crocosmia x crocosmiiflora OX=1053288 GN=FLS1 PE=1 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.6e-15
Identity = 42/120 (35.00%), Postives = 61/120 (50.83%), Query Frame = 0


Query: 46  PLAEIPVVDLSQLSLPSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFS 105
           P+ EIPV+DL         +G +  +  A   WG FQ INH I    +++++++ ++FF 
Sbjct: 39  PIPEIPVIDLGD------KDGIVGAVAEAAREWGLFQIINHGIPVEVIKELQRVGKEFFE 98

Query: 106 LPIEEKMRYGREVDGMEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSFR 165
           LP EEK  Y  ++  MEGYG  L    +    W D L+    PE       WP NPP +R
Sbjct: 99  LPQEEKEVYAMDMVTMEGYGTKLQKETEGKKAWVDHLFHNIWPESRINYNFWPQNPPDYR 152

[Close]

BLAST of CSPI01G17050 vs. ExPASy TrEMBL
Match: A0A0A0LWH8 (DIOX_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G263960 PE=4 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 3.0e-86
Identity = 159/164 (96.95%), Postives = 159/164 (96.95%), Query Frame = 0


Query: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSL 60
           MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLS 
Sbjct: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSS 60

Query: 61  PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120
           PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG
Sbjct: 61  PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120

Query: 121 MEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSF 165
           MEGYGNDL  S QQ LDWSDRLYFVTNPEDERRLELWPLNPPSF
Sbjct: 121 MEGYGNDLTFSNQQTLDWSDRLYFVTNPEDERRLELWPLNPPSF 164

[Close]

BLAST of CSPI01G17050 vs. ExPASy TrEMBL
Match: A0A1S3CP07 (protein SRG1-like OS=Cucumis melo OX=3656 GN=LOC103503159 PE=3 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 2.1e-79
Identity = 148/168 (88.10%), Postives = 153/168 (91.07%), Query Frame = 0


Query: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSL 60
           MAQNSQ  QTQTLQQQLLINGGHTPESYIYKGGYHGG SNNNTPLPLA+IPV+DLSQLS 
Sbjct: 1   MAQNSQIPQTQTLQQQLLINGGHTPESYIYKGGYHGGDSNNNTPLPLAQIPVLDLSQLSS 60

Query: 61  PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120
            S GE PLN LRLALSTWGCFQA NH ISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG
Sbjct: 61  ASVGEAPLNHLRLALSTWGCFQATNHGISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120

Query: 121 MEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSFRNNV 169
           MEGYGNDLI S QQILDWSDRLY VTNP+DERRLE WPLNPPSFR ++
Sbjct: 121 MEGYGNDLIFSNQQILDWSDRLYLVTNPKDERRLEFWPLNPPSFREDL 168

[Close]

BLAST of CSPI01G17050 vs. ExPASy TrEMBL
Match: A0A5D3CEG2 (Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold367G00240 PE=3 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 1.3e-78
Identity = 147/164 (89.63%), Postives = 150/164 (91.46%), Query Frame = 0


Query: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSL 60
           MAQNSQ  QTQTLQQQLLINGGHTPESYIYKGGYHGG SNNNTPLPLA+IPV+DLSQLS 
Sbjct: 1   MAQNSQIPQTQTLQQQLLINGGHTPESYIYKGGYHGGDSNNNTPLPLAQIPVLDLSQLSS 60

Query: 61  PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120
            S GE PLN LRLALSTWGCFQA NH ISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG
Sbjct: 61  ASVGEAPLNHLRLALSTWGCFQATNHGISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120

Query: 121 MEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSF 165
           MEGYGNDLI S QQILDWSDRLY VTNP+DERRLE WPLNPPSF
Sbjct: 121 MEGYGNDLIFSNQQILDWSDRLYLVTNPKDERRLEFWPLNPPSF 164

[Close]

BLAST of CSPI01G17050 vs. ExPASy TrEMBL
Match: A0A5A7U2F3 (Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00670 PE=3 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 3.0e-78
Identity = 147/164 (89.63%), Postives = 149/164 (90.85%), Query Frame = 0


Query: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSL 60
           MAQNSQ  QTQTLQQQLLINGGHTPESYIYKGGYHGG SNNNTPLPLA+IPV+DLSQLS 
Sbjct: 1   MAQNSQIPQTQTLQQQLLINGGHTPESYIYKGGYHGGDSNNNTPLPLAQIPVLDLSQLSS 60

Query: 61  PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120
            S GE PLN LRLALSTWGCFQA NH ISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG
Sbjct: 61  ASVGEAPLNHLRLALSTWGCFQATNHGISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120

Query: 121 MEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSF 165
           MEGYGNDLI S QQILDWSDRLY VTNPED RRLE WPLNPPSF
Sbjct: 121 MEGYGNDLIFSNQQILDWSDRLYLVTNPEDGRRLEFWPLNPPSF 164

[Close]

BLAST of CSPI01G17050 vs. ExPASy TrEMBL
Match: A0A6J1BYU1 (uncharacterized protein LOC111006992 OS=Momordica charantia OX=3673 GN=LOC111006992 PE=4 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 2.7e-55
Identity = 111/168 (66.07%), Postives = 131/168 (77.98%), Query Frame = 0


Query: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSL 60
           MA+      T+T QQ+LLINGG TPESYIYK GY GGG +NN PLPLAEIPVVDL+QLS 
Sbjct: 353 MAELPDYFHTKTFQQKLLINGGDTPESYIYKDGY-GGGDSNNNPLPLAEIPVVDLAQLSS 412

Query: 61  PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120
                  L DLRLAL++WGCFQAINHSISSSFL K+ +IS QFFSLP+EEK +  RE+ G
Sbjct: 413 SPPSTAALEDLRLALTSWGCFQAINHSISSSFLNKIHQISNQFFSLPMEEKNKCCRELYG 472

Query: 121 MEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSFRNNV 169
           +EGYG D++ SEQQILDW+DRLY   NPEDER+L+ WP NP SFR ++
Sbjct: 473 LEGYGTDMVFSEQQILDWTDRLYLKVNPEDERQLKYWPQNPQSFREDL 519

[Close]

BLAST of CSPI01G17050 vs. NCBI nr
Match: XP_011655698.2 (probable 2-oxoglutarate-dependent dioxygenase ANS [Cucumis sativus] >KAE8653028.1 hypothetical protein Csa_020037 [Cucumis sativus])

HSP 1 Score: 338.2 bits (866), Expect = 4.6e-89
Identity = 164/170 (96.47%), Postives = 167/170 (98.24%), Query Frame = 0


Query: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSL 60
           MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLS 
Sbjct: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSS 60

Query: 61  PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120
           PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG
Sbjct: 61  PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120

Query: 121 MEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSFRNNVGD 171
           MEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSFR ++ +
Sbjct: 121 MEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSFREDLDE 170

[Close]

BLAST of CSPI01G17050 vs. NCBI nr
Match: XP_011655280.1 (probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 [Cucumis sativus] >KAE8653014.1 hypothetical protein Csa_004557 [Cucumis sativus])

HSP 1 Score: 324.3 bits (830), Expect = 6.8e-85
Identity = 156/168 (92.86%), Postives = 161/168 (95.83%), Query Frame = 0


Query: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSL 60
           MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLS 
Sbjct: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSS 60

Query: 61  PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120
           PSAGEGPLNDLRLALSTWGCFQA NHSISSSFLEK+RKISEQFFSLPIEEKMRYGREVDG
Sbjct: 61  PSAGEGPLNDLRLALSTWGCFQATNHSISSSFLEKLRKISEQFFSLPIEEKMRYGREVDG 120

Query: 121 MEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSFRNNV 169
           MEGYGNDL  S QQ LDWSDRLYFVT+PEDERRL+LWPLNPPSFR ++
Sbjct: 121 MEGYGNDLTFSNQQTLDWSDRLYFVTSPEDERRLDLWPLNPPSFREDL 168

[Close]

BLAST of CSPI01G17050 vs. NCBI nr
Match: XP_031736054.1 (probable 2-oxoglutarate-dependent dioxygenase ANS isoform X1 [Cucumis sativus])

HSP 1 Score: 321.6 bits (823), Expect = 4.4e-84
Identity = 155/164 (94.51%), Postives = 158/164 (96.34%), Query Frame = 0


Query: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSL 60
           MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLS 
Sbjct: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSS 60

Query: 61  PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120
           PSAGEGPLNDLRLALSTWGCFQA NHSISSSFLEK+RKISEQFFSLPIEEKMRYGREVDG
Sbjct: 61  PSAGEGPLNDLRLALSTWGCFQATNHSISSSFLEKLRKISEQFFSLPIEEKMRYGREVDG 120

Query: 121 MEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSF 165
           MEGYGNDL  S QQ LDWSDRLYFVT+PEDERRL+LWPLNPPSF
Sbjct: 121 MEGYGNDLTFSNQQTLDWSDRLYFVTSPEDERRLDLWPLNPPSF 164

[Close]

BLAST of CSPI01G17050 vs. NCBI nr
Match: XP_008465534.1 (PREDICTED: protein SRG1-like [Cucumis melo])

HSP 1 Score: 305.1 bits (780), Expect = 4.3e-79
Identity = 148/168 (88.10%), Postives = 153/168 (91.07%), Query Frame = 0


Query: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSL 60
           MAQNSQ  QTQTLQQQLLINGGHTPESYIYKGGYHGG SNNNTPLPLA+IPV+DLSQLS 
Sbjct: 1   MAQNSQIPQTQTLQQQLLINGGHTPESYIYKGGYHGGDSNNNTPLPLAQIPVLDLSQLSS 60

Query: 61  PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120
            S GE PLN LRLALSTWGCFQA NH ISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG
Sbjct: 61  ASVGEAPLNHLRLALSTWGCFQATNHGISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120

Query: 121 MEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSFRNNV 169
           MEGYGNDLI S QQILDWSDRLY VTNP+DERRLE WPLNPPSFR ++
Sbjct: 121 MEGYGNDLIFSNQQILDWSDRLYLVTNPKDERRLEFWPLNPPSFREDL 168

[Close]

BLAST of CSPI01G17050 vs. NCBI nr
Match: TYK10347.1 (protein SRG1-like [Cucumis melo var. makuwa])

HSP 1 Score: 302.4 bits (773), Expect = 2.8e-78
Identity = 147/164 (89.63%), Postives = 150/164 (91.46%), Query Frame = 0


Query: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSL 60
           MAQNSQ  QTQTLQQQLLINGGHTPESYIYKGGYHGG SNNNTPLPLA+IPV+DLSQLS 
Sbjct: 1   MAQNSQIPQTQTLQQQLLINGGHTPESYIYKGGYHGGDSNNNTPLPLAQIPVLDLSQLSS 60

Query: 61  PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120
            S GE PLN LRLALSTWGCFQA NH ISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG
Sbjct: 61  ASVGEAPLNHLRLALSTWGCFQATNHGISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120

Query: 121 MEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSF 165
           MEGYGNDLI S QQILDWSDRLY VTNP+DERRLE WPLNPPSF
Sbjct: 121 MEGYGNDLIFSNQQILDWSDRLYLVTNPKDERRLEFWPLNPPSF 164

[Close]

BLAST of CSPI01G17050 vs. TAIR 10
Match: AT5G20550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 142.5 bits (358), Expect = 3.4e-34
Identity = 68/155 (43.87%), Postives = 98/155 (63.23%), Query Frame = 0


Query: 15  QQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSLPS-AGEGPLNDLRL 74
           Q+++  G   PE Y+        G + N  +P+ +IP +DLS L  PS  G   L+ L  
Sbjct: 10  QEVVAAGEGIPERYLQPPAVDDNGQHLNAAVPVMDIPAIDLSLLLSPSDDGREELSKLHS 69

Query: 75  ALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDGMEGYGNDLILSEQ 134
           ALSTWG  Q INH I+ + L+K+ K++++F +LP EEK +Y RE+  ++GYGND+IL + 
Sbjct: 70  ALSTWGVVQVINHGITKALLDKIYKLTKEFCALPSEEKQKYAREIGSIQGYGNDMILWDD 129

Query: 135 QILDWSDRLYFVTNPEDERRLELWPLNPPSFRNNV 169
           Q+LDW DRLY  T PED+R+L+ WP  P  FR  +
Sbjct: 130 QVLDWIDRLYITTYPEDQRQLKFWPDVPVGFRETL 164

[Close]

BLAST of CSPI01G17050 vs. TAIR 10
Match: AT5G54000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 141.0 bits (354), Expect = 1.0e-33
Identity = 68/156 (43.59%), Postives = 99/156 (63.46%), Query Frame = 0


Query: 15  QQLLINGGHTPESYIYKGGYHGGGSNN-NTPLPLAEIPVVDLSQL-SLPSAGEGPLNDLR 74
           Q+++  G   PE Y+Y     G G    N  LP  +I ++DL+ L S    G   L+ L 
Sbjct: 10  QEVVAAGEKLPERYLYTPTGDGEGDQPFNGLLPEMKISIIDLNLLFSSSDDGREELSKLH 69

Query: 75  LALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDGMEGYGNDLILSE 134
            A+STWG  Q +NH IS + L+K+ ++++QFF LP +EK +Y RE+   +G+GND+ILS+
Sbjct: 70  SAISTWGVVQVMNHGISEALLDKIHELTKQFFVLPTKEKQKYAREISSFQGFGNDMILSD 129

Query: 135 QQILDWSDRLYFVTNPEDERRLELWPLNPPSFRNNV 169
            Q+LDW DRLY +T PED+R+L+ WP NP  FR  +
Sbjct: 130 DQVLDWVDRLYLITYPEDQRQLKFWPENPSGFRETL 165

[Close]

BLAST of CSPI01G17050 vs. TAIR 10
Match: AT5G20400.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 141.0 bits (354), Expect = 1.0e-33
Identity = 68/155 (43.87%), Postives = 99/155 (63.87%), Query Frame = 0


Query: 15  QQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLS-QLSLPSAGEGPLNDLRL 74
           Q+++  G   PE Y++     G     N  +P  +IP +DL+  LS   AG+  L+ L  
Sbjct: 10  QEVVAAGEGLPERYLHAPTGDGEVQPLNAAVPEMDIPAIDLNLLLSSSEAGQQELSKLHS 69

Query: 75  ALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDGMEGYGNDLILSEQ 134
           ALSTWG  Q +NH I+ +FL+K+ K++++FF+LP EEK +  RE+D ++GYGND+IL + 
Sbjct: 70  ALSTWGVVQVMNHGITKAFLDKIYKLTKEFFALPTEEKQKCAREIDSIQGYGNDMILWDD 129

Query: 135 QILDWSDRLYFVTNPEDERRLELWPLNPPSFRNNV 169
           Q+LDW DRLY  T PED+R+L  WP  P  FR  +
Sbjct: 130 QVLDWIDRLYITTYPEDQRQLNFWPEVPLGFRETL 164

[Close]

BLAST of CSPI01G17050 vs. TAIR 10
Match: AT1G49390.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 136.0 bits (341), Expect = 3.2e-32
Identity = 66/157 (42.04%), Postives = 97/157 (61.78%), Query Frame = 0


Query: 15  QQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSLPSA-GEGPLNDLRL 74
           Q+++  G   PE Y++     G     N  +P  +IP +DLS L   S  G+  +  L  
Sbjct: 10  QEVVAAGQGLPERYLHAPTGEGESQPLNGAVPEMDIPAIDLSLLFSSSVDGQEEMKKLHS 69

Query: 75  ALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDGMEGYGNDLILSEQ 134
           ALSTWG  Q +NH I+ +FL+K+ K+++QFF+LP EEK +  RE   ++GYGND+ILS+ 
Sbjct: 70  ALSTWGVVQVMNHGITEAFLDKIYKLTKQFFALPTEEKHKCARETGNIQGYGNDMILSDN 129

Query: 135 QILDWSDRLYFVTNPEDERRLELWPLNPPSFRNNVGD 171
           Q+LDW DRL+  T PED+R+L+ WP  P  F   + +
Sbjct: 130 QVLDWIDRLFLTTYPEDKRQLKFWPQVPVGFSETLDE 166

[Close]

BLAST of CSPI01G17050 vs. TAIR 10
Match: AT1G78550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 103.2 bits (256), Expect = 2.3e-22
Identity = 52/137 (37.96%), Postives = 77/137 (56.20%), Query Frame = 0


Query: 48  AEIPVVDLSQLSLPSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLP 107
           +EIPV+D+++L   SA +  L  L  A   WG FQ +NH I SSFLEK+    ++FF+LP
Sbjct: 51  SEIPVIDMTRLCSVSAMDSELKKLDFACQDWGFFQLVNHGIDSSFLEKLETEVQEFFNLP 110

Query: 108 IEEKMRYGREVDGMEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSFRNN 167
           ++EK +  +     EG+G   I+SE Q LDW D     T P   R+  L+   PP FR  
Sbjct: 111 MKEKQKLWQRSGEFEGFGQVNIVSENQKLDWGDMFILTTEPIRSRKSHLFSKLPPPFRET 170

Query: 168 VGDTFPGTCDANASVIF 185
           + +T+     + A ++F
Sbjct: 171 L-ETYSSEVKSIAKILF 186

The following BLAST results are available for this feature:

BLAST of CSPI01G17050 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A2A1A0	1.7e-22	42.50	S-norcoclaurine synthase 1 OS=Coptis japonica OX=3442 GN=NCS1 PE=1 SV=1	[more]
Q94LP4	9.4e-21	36.30	2-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947...	[more]
Q39224	2.7e-20	36.67	Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1	[more]
O80449	2.4e-16	37.61	Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1	[more]
A0A4D6Q440	1.6e-15	35.00	Flavonol synthase 1 OS=Crocosmia x crocosmiiflora OX=1053288 GN=FLS1 PE=1 SV=1	[more]

BLAST of CSPI01G17050 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LWH8	3.0e-86	96.95	DIOX_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G263960 PE=4...	[more]
A0A1S3CP07	2.1e-79	88.10	protein SRG1-like OS=Cucumis melo OX=3656 GN=LOC103503159 PE=3 SV=1	[more]
A0A5D3CEG2	1.3e-78	89.63	Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold367G00...	[more]
A0A5A7U2F3	3.0e-78	89.63	Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00...	[more]
A0A6J1BYU1	2.7e-55	66.07	uncharacterized protein LOC111006992 OS=Momordica charantia OX=3673 GN=LOC111006...	[more]

BLAST of CSPI01G17050 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011655698.2	4.6e-89	96.47	probable 2-oxoglutarate-dependent dioxygenase ANS [Cucumis sativus] >KAE8653028....	[more]
XP_011655280.1	6.8e-85	92.86	probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 [Cucumis sativus] >...	[more]
XP_031736054.1	4.4e-84	94.51	probable 2-oxoglutarate-dependent dioxygenase ANS isoform X1 [Cucumis sativus]	[more]
XP_008465534.1	4.3e-79	88.10	PREDICTED: protein SRG1-like [Cucumis melo]	[more]
TYK10347.1	2.8e-78	89.63	protein SRG1-like [Cucumis melo var. makuwa]	[more]

BLAST of CSPI01G17050 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20550.1	3.4e-34	43.87	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G54000.1	1.0e-33	43.59	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G20400.1	1.0e-33	43.87	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G49390.1	3.2e-32	42.04	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G78550.1	2.3e-22	37.96	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 10..182 e-value: 3.8E-31 score: 110.5
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 50..158 e-value: 5.7E-23 score: 81.9
None	No IPR available	PANTHER	PTHR10209:SF454	SUBFAMILY NOT NAMED	coord: 1..166
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 1..166
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 13..168

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G17050.1	CSPI01G17050.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016491	oxidoreductase activity