CSPI01G14800 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G14800
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionReverse transcriptase
LocationChr1: 10477172 .. 10477701 (-)
RNA-Seq ExpressionCSPI01G14800
SyntenyCSPI01G14800
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGCGAGGAAGAGGACATGGACGACTAGGGAGCAAGCCAAGCACAACAACTGAGCCCACTATGGACTCAGAGGCCCAGTCAGTAAATTCAAATGTTAAGAGAGTGATAGAAGAGGAGTTATATAATCGAGTGGTGCAAAATTAAAAGATAGTTTATCAAGAAAGCCGAAGAAGAAGTATGGAATAGAGCAAGTCAAGGCATTTGGAGCCAAAGAGTTTTCAAGAACAGTAGAACTAGAGCCGGCGAATAAGTGGTTAAGAGCATTGAAGAAGTGTTTTAGGGTACTGCAGTGCCCCGAGGAGAGGAAGATTGACTTAGCAGCTTTCTTACTTCATAGGAAAGCAGAAGACCGGCAGGTGTTAGAAGAAAGTAGACGAGGCGAGGTGAATTGGGAGGAGTTTAAGGCAGTGTTTAATGAGAAGTATTTCTTAGAGACTTATAAAGAGGGGAGGCAAGATGAACTTTTCCACCTAGAGCAAGGTGAGCGAGGTCACAGAGTATGCGAAGAAGTTTATGGAGTTGGCTAA

mRNA sequence

ATGTTGCGAGGAAGAGGACATGGACGACTAGGGAGCAAGCCAAGCACAACAACTGAGCCCACTATGGACTCAGAGGCCCAGTCACCGAAGAAGAAGTATGGAATAGAGCAAGTCAAGGCATTTGGAGCCAAAGAGTTTTCAAGAACAGTAGAACTAGAGCCGGCGAATAAGTGGTTAAGAGCATTGAAGAAGTGTTTTAGGGTACTGCAGTGCCCCGAGGAGAGGAAGATTGACTTAGCAGCTTTCTTACTTCATAGGAAAGCAGAAGACCGGCAGGTGTTAGAAGAAAGTAGACGAGGCGAGGTGAATTGGGAGGAGTTTAAGGCAGTGTTTAATGAGAAGTATTTCTTAGAGACTTATAAAGAGGGGAGGCAAGATGAACTTTTCCACCTAGAGCAAGGTGAGCGAGGTCACAGAGTATGCGAAGAAGTTTATGGAGTTGGCTAA

Coding sequence (CDS)

ATGTTGCGAGGAAGAGGACATGGACGACTAGGGAGCAAGCCAAGCACAACAACTGAGCCCACTATGGACTCAGAGGCCCAGTCACCGAAGAAGAAGTATGGAATAGAGCAAGTCAAGGCATTTGGAGCCAAAGAGTTTTCAAGAACAGTAGAACTAGAGCCGGCGAATAAGTGGTTAAGAGCATTGAAGAAGTGTTTTAGGGTACTGCAGTGCCCCGAGGAGAGGAAGATTGACTTAGCAGCTTTCTTACTTCATAGGAAAGCAGAAGACCGGCAGGTGTTAGAAGAAAGTAGACGAGGCGAGGTGAATTGGGAGGAGTTTAAGGCAGTGTTTAATGAGAAGTATTTCTTAGAGACTTATAAAGAGGGGAGGCAAGATGAACTTTTCCACCTAGAGCAAGGTGAGCGAGGTCACAGAGTATGCGAAGAAGTTTATGGAGTTGGCTAA

Protein sequence

MLRGRGHGRLGSKPSTTTEPTMDSEAQSPKKKYGIEQVKAFGAKEFSRTVELEPANKWLRALKKCFRVLQCPEERKIDLAAFLLHRKAEDRQVLEESRRGEVNWEEFKAVFNEKYFLETYKEGRQDELFHLEQGERGHRVCEEVYGVG*
Homology
BLAST of CSPI01G14800 vs. ExPASy TrEMBL
Match: A0A0A0LVF8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G165220 PE=4 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 4.1e-22
Identity = 59/80 (73.75%), Postives = 62/80 (77.50%), Query Frame = 0

Query: 11  GSKPSTTTEPTMDSEAQSPKKKYGIEQVKAFGAKEFSRTVELEPANKWLRALKKCFRVLQ 70
           G+  +    P         KKKYGIEQVKAFG KEFSRTVELE ANKWLRAL+KCFRVLQ
Sbjct: 35  GASQAQQLSPLWTQRPSHRKKKYGIEQVKAFGDKEFSRTVELEQANKWLRALEKCFRVLQ 94

Query: 71  CPEERKIDLAAFLLHRKAED 91
           C EERKIDLAAFLLHRKAED
Sbjct: 95  CLEERKIDLAAFLLHRKAED 114

BLAST of CSPI01G14800 vs. ExPASy TrEMBL
Match: A0A5A7TBS0 (CCHC-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G002900 PE=4 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 2.3e-20
Identity = 52/115 (45.22%), Postives = 77/115 (66.96%), Query Frame = 0

Query: 23  DSEAQSPKKKYGIEQVKAFGAKEFSRTVELEPANKWLRALKKCFRVLQCPEERKIDLAAF 82
           +S    PKKKYGIE++KA GA  F+ T        WL  ++KCFRV +CPE+RK++LAAF
Sbjct: 140 ESAQSDPKKKYGIERLKALGATTFAGTTNPTDVEAWLTLIEKCFRVTRCPEDRKVELAAF 199

Query: 83  LLHRKAEDRQVLEESRR---GEVNWEEFKAVFNEKYFLETYKEGRQDELFHLEQG 135
           LL   AED   +EESRR   G+++W+EFK  F +K++  ++++ +++E   L QG
Sbjct: 200 LLQNGAEDWWRMEESRRRTTGDISWDEFKKAFFDKFYPRSFRDAKRNEFLRLTQG 254

BLAST of CSPI01G14800 vs. ExPASy TrEMBL
Match: A0A5D3CTK6 (CCHC-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold892G00030 PE=4 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 2.3e-20
Identity = 52/115 (45.22%), Postives = 77/115 (66.96%), Query Frame = 0

Query: 23  DSEAQSPKKKYGIEQVKAFGAKEFSRTVELEPANKWLRALKKCFRVLQCPEERKIDLAAF 82
           +S    PKKKYGIE++KA GA  F+ T        WL  ++KCFRV +CPE+RK++LAAF
Sbjct: 176 ESAQSDPKKKYGIERLKALGATTFAGTTNPTDVEAWLTLIEKCFRVTRCPEDRKVELAAF 235

Query: 83  LLHRKAEDRQVLEESRR---GEVNWEEFKAVFNEKYFLETYKEGRQDELFHLEQG 135
           LL   AED   +EESRR   G+++W+EFK  F +K++  ++++ +++E   L QG
Sbjct: 236 LLQNGAEDWWRMEESRRRTTGDISWDEFKKAFFDKFYPRSFRDAKRNEFLRLTQG 290

BLAST of CSPI01G14800 vs. ExPASy TrEMBL
Match: A0A5A7UZM6 (Gag protease polyprotein-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold119G00750 PE=4 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 3.9e-20
Identity = 52/115 (45.22%), Postives = 77/115 (66.96%), Query Frame = 0

Query: 23  DSEAQSPKKKYGIEQVKAFGAKEFSRTVELEPANKWLRALKKCFRVLQCPEERKIDLAAF 82
           +S    P+KKYGIE++KA GA  F+ T     A  WL  ++KCFRV +CPE+RK++LAAF
Sbjct: 46  ESTQSDPEKKYGIERLKALGATTFAGTTNPADAEAWLTLIEKCFRVTRCPEDRKVELAAF 105

Query: 83  LLHRKAEDRQVLEESRR---GEVNWEEFKAVFNEKYFLETYKEGRQDELFHLEQG 135
           LL   AED   +EESRR   G+++W EFK  F +K++  ++++ +++E   L QG
Sbjct: 106 LLQNGAEDWWRMEESRRRTTGDISWNEFKKAFFDKFYPRSFRDAKRNEFLRLTQG 160

BLAST of CSPI01G14800 vs. ExPASy TrEMBL
Match: A0A5A7SRT9 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold478G00220 PE=4 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 8.9e-17
Identity = 49/124 (39.52%), Postives = 73/124 (58.87%), Query Frame = 0

Query: 25  EAQSPKKKYGIEQVKAFGAKEFSRTVELEPANKWLRALKKCFRVLQCPEERKIDLAAFLL 84
           E   P+K YGIE++K  GA  F  +++   A  WL  L+KCF V+ CPEERK+ LA FLL
Sbjct: 41  EPSDPEKAYGIERLKKLGATVFEGSIDPADAENWLNMLEKCFDVMNCPEERKVRLAIFLL 100

Query: 85  HRKAEDRQVLEESRRGE---VNWEEFKAVFNEKYFLETYKEGRQDELFHLEQGERGHRVC 144
            ++AE       +RR +   ++W+ F+ +F +KY+  TY + ++DE   L+QG      C
Sbjct: 101 QKEAEGWWKCILARRCDARALDWQNFRGIFEDKYYPSTYCKAKRDEFLGLKQGSLSVAEC 160

Query: 145 EEVY 146
           E  Y
Sbjct: 161 ERKY 164

BLAST of CSPI01G14800 vs. NCBI nr
Match: KGN64939.1 (hypothetical protein Csa_022824 [Cucumis sativus])

HSP 1 Score: 114.4 bits (285), Expect = 8.5e-22
Identity = 59/80 (73.75%), Postives = 62/80 (77.50%), Query Frame = 0

Query: 11  GSKPSTTTEPTMDSEAQSPKKKYGIEQVKAFGAKEFSRTVELEPANKWLRALKKCFRVLQ 70
           G+  +    P         KKKYGIEQVKAFG KEFSRTVELE ANKWLRAL+KCFRVLQ
Sbjct: 35  GASQAQQLSPLWTQRPSHRKKKYGIEQVKAFGDKEFSRTVELEQANKWLRALEKCFRVLQ 94

Query: 71  CPEERKIDLAAFLLHRKAED 91
           C EERKIDLAAFLLHRKAED
Sbjct: 95  CLEERKIDLAAFLLHRKAED 114

BLAST of CSPI01G14800 vs. NCBI nr
Match: TYK15233.1 (uncharacterized protein E5676_scaffold892G00030 [Cucumis melo var. makuwa])

HSP 1 Score: 108.6 bits (270), Expect = 4.7e-20
Identity = 52/115 (45.22%), Postives = 77/115 (66.96%), Query Frame = 0

Query: 23  DSEAQSPKKKYGIEQVKAFGAKEFSRTVELEPANKWLRALKKCFRVLQCPEERKIDLAAF 82
           +S    PKKKYGIE++KA GA  F+ T        WL  ++KCFRV +CPE+RK++LAAF
Sbjct: 176 ESAQSDPKKKYGIERLKALGATTFAGTTNPTDVEAWLTLIEKCFRVTRCPEDRKVELAAF 235

Query: 83  LLHRKAEDRQVLEESRR---GEVNWEEFKAVFNEKYFLETYKEGRQDELFHLEQG 135
           LL   AED   +EESRR   G+++W+EFK  F +K++  ++++ +++E   L QG
Sbjct: 236 LLQNGAEDWWRMEESRRRTTGDISWDEFKKAFFDKFYPRSFRDAKRNEFLRLTQG 290

BLAST of CSPI01G14800 vs. NCBI nr
Match: KAA0039476.1 (uncharacterized protein E6C27_scaffold64G002900 [Cucumis melo var. makuwa])

HSP 1 Score: 108.6 bits (270), Expect = 4.7e-20
Identity = 52/115 (45.22%), Postives = 77/115 (66.96%), Query Frame = 0

Query: 23  DSEAQSPKKKYGIEQVKAFGAKEFSRTVELEPANKWLRALKKCFRVLQCPEERKIDLAAF 82
           +S    PKKKYGIE++KA GA  F+ T        WL  ++KCFRV +CPE+RK++LAAF
Sbjct: 140 ESAQSDPKKKYGIERLKALGATTFAGTTNPTDVEAWLTLIEKCFRVTRCPEDRKVELAAF 199

Query: 83  LLHRKAEDRQVLEESRR---GEVNWEEFKAVFNEKYFLETYKEGRQDELFHLEQG 135
           LL   AED   +EESRR   G+++W+EFK  F +K++  ++++ +++E   L QG
Sbjct: 200 LLQNGAEDWWRMEESRRRTTGDISWDEFKKAFFDKFYPRSFRDAKRNEFLRLTQG 254

BLAST of CSPI01G14800 vs. NCBI nr
Match: KAA0060484.1 (Gag protease polyprotein-like protein [Cucumis melo var. makuwa] >TYK18569.1 Gag protease polyprotein-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 107.8 bits (268), Expect = 8.0e-20
Identity = 52/115 (45.22%), Postives = 77/115 (66.96%), Query Frame = 0

Query: 23  DSEAQSPKKKYGIEQVKAFGAKEFSRTVELEPANKWLRALKKCFRVLQCPEERKIDLAAF 82
           +S    P+KKYGIE++KA GA  F+ T     A  WL  ++KCFRV +CPE+RK++LAAF
Sbjct: 46  ESTQSDPEKKYGIERLKALGATTFAGTTNPADAEAWLTLIEKCFRVTRCPEDRKVELAAF 105

Query: 83  LLHRKAEDRQVLEESRR---GEVNWEEFKAVFNEKYFLETYKEGRQDELFHLEQG 135
           LL   AED   +EESRR   G+++W EFK  F +K++  ++++ +++E   L QG
Sbjct: 106 LLQNGAEDWWRMEESRRRTTGDISWNEFKKAFFDKFYPRSFRDAKRNEFLRLTQG 160

BLAST of CSPI01G14800 vs. NCBI nr
Match: XP_038877203.1 (uncharacterized protein LOC120069501 [Benincasa hispida])

HSP 1 Score: 99.4 bits (246), Expect = 2.8e-17
Identity = 46/119 (38.66%), Postives = 80/119 (67.23%), Query Frame = 0

Query: 19  EPTMDSEAQSPKKKYGIEQVKAFGAKEFSRTVELEPANKWLRALKKCFRVLQCPEERKID 78
           + ++++    P+KKYGIE++KA GA  F  T +   A +W+  ++KCF+V++CP++RK+ 
Sbjct: 9   QASLENIQPDPEKKYGIERLKALGATNFEGTKDPADAEEWMNQIEKCFKVMRCPKKRKVS 68

Query: 79  LAAFLLHRKAEDRQVLEESRRG---EVNWEEFKAVFNEKYFLETYKEGRQDELFHLEQG 135
           LA FLL +K ED   L ++R+G   E+ WEEF+  F E+++  ++++ +QDE   + QG
Sbjct: 69  LATFLLQKKVEDWWKLLKNRKGDQEEICWEEFQKSFFERFYPRSFRDMKQDEFLQVMQG 127

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0LVF84.1e-2273.75Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G165220 PE=4 SV=1[more]
A0A5A7TBS02.3e-2045.22CCHC-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
A0A5D3CTK62.3e-2045.22CCHC-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A5A7UZM63.9e-2045.22Gag protease polyprotein-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A5A7SRT98.9e-1739.52Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold47... [more]
Match NameE-valueIdentityDescription
KGN64939.18.5e-2273.75hypothetical protein Csa_022824 [Cucumis sativus][more]
TYK15233.14.7e-2045.22uncharacterized protein E5676_scaffold892G00030 [Cucumis melo var. makuwa][more]
KAA0039476.14.7e-2045.22uncharacterized protein E6C27_scaffold64G002900 [Cucumis melo var. makuwa][more]
KAA0060484.18.0e-2045.22Gag protease polyprotein-like protein [Cucumis melo var. makuwa] >TYK18569.1 Gag... [more]
XP_038877203.12.8e-1738.66uncharacterized protein LOC120069501 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..31

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G14800.1CSPI01G14800.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043170 macromolecule metabolic process
biological_process GO:0006807 nitrogen compound metabolic process
biological_process GO:0044238 primary metabolic process