CSPI01G14660 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G14660
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Aspergillus nuclease S(1)
Location	Chr1: 10374403 .. 10378231 (+)
RNA-Seq Expression	CSPI01G14660
Synteny	CSPI01G14660

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTAACTGTCCGTTTTTGTGATTCTAATTTTCGTAGCCTCTGATTTGGTGAGAGGCAAGCGTCGAAAAACGAGAAACAAGGCTGGTTTAAATTTGAATTTGGAGACAAGCTTCTAAGCCAGAGGTCAAGCAGCTGAAGTCGGAGCTGCAATCGCTGCCAGCAACTGATTTCTGATTTCATTGTGTTTATCATTACCGTATTTTGTTTCTAGGGAGCAGACATGGGTCAGTCTGAGCTTTGTTGGACTGCCAATGCTTTCCTTTTTCTCTTACTTCTACCTGGAATCCTCGGCTGGGGAAGGGAAGGTCACTATATGATTTGCAAGATAGCAGAGGTAACCATACTTTTGAGCATTATTTCCGTTTAGTTTTATCTAGCCTTTCGGTGTAACGCTGTTAATAGGCAAGGATTGCAATTGAAGGGATTTGGGTTTCGAATTCTTGAAACTGTAATAGCGTAGACAAGGACTCAAATTAAAAGAAACATGACAAAGGGTTTTGGAGCTGATTATGACTCAAACCCTATCATTAAGATTCCAACCATTAGATCCTGTATAAAGTTTGTTGAAGCCAAAATCTGATATCACTTCCTTTGGTTTAGAAATATTTGACTGAAGATGCTCTATCAATGGTCAAAGAATTGCTTCCATCTTATGCTGAGGGCGATCTTGCAGCTGTATGCTCCTGGGCTGATGAACTTCGAGCACATCCTGATTATCACTGGAGTGGCGCCTTACACTATGTTGACACGCCAGATTTCTTTTGTAATTATAAATGCTCGAGTAAGGTTAAGCTTTATGTTATTTTCAGTTTCTCTTGCAGTCTATCATGCCGTGTCGCTCTTTGATGTAATTCTTATTATCATTGTGTTCATGAGCTTTAAATTCACATTCATTGTTGAATCACAAGAGCACTCTTGATACTCGTAAATTTTGTAGTTCATGATCACATATATGATGAATCCTCCGATGTTATACCGTTAATTTCACCATGGTCTGTCTGATTTGGATATATATAGTATCAGTGTCTTCCTAACAAACTCGTTCTGCAATTTAGTGAGTGTGTGTGTCATTATGGCTATGTCATATATGATGGTTATTAAATTCAATTGGTTTTTTATTTTGGTCATTGAACGAGGTATCAAGATTTATCTCAAATAACTCATTTTCAATACTTGAAACTTGAAAGTATATTTGATGATGTTGGCCTATGTAACAGTATTTTGTGCTTCTTGACAGGAGACTGTCATGACAATTATAGACACAAAGGTAGATGTGTGACAGCAGCAATTTACAACTACACTATGCAACTCGAATCAGCTTACAAGGAAATAACTTCAGAAATTAAATGTAAGCATCCTAGGTGACTGACGTTGTATCTGTACACTTCTCGAACAAATGTCATTTTTATTCACATGATATTTTCAGATAACTTAACAGAGGCTCTTATGTTCTTGTCCCATTTTATCGGAGACGTTCATCAGGTTTGCACTTTCTACAAATTTGTACTTAAAGGCAGAGATAAATGAAAATAGAAAATAGATTCATGAAATTTGTATAGGTGCAAAATTTACTGCTCTGAGTGTTGTTGAAAGTTCACTTTCACTCGATTGGCTGTTCAAGTCCCTCATCCTTTTTAAATAAGATTTCTTGGAGTTGGACTTGCAGGTTTGTACATTGATTACATAGATAAATTATAGCCTCATTAATGGAGTTTCACCTTTTTCCATTACAGCACTTAAATTCTATAATAGTACTTCTTGAAAGTCTTCAATGGTACTGGTTGCTAATAATAACAATGTCTTCTTTATTTTATTTTTCGAAAAAGAGTTGGATAATCAATGGTTTTATCTCTTGCATCTGACTTTCCCGATATTCAATGGCAGCCCCTTCATGTCGGTTTTGTCGGAGATATAGGAGGTAATTTAATAAAAGTCAGTTGGTACCGAAGAAGGACCAATCTCCATCATGTAAGTGTCATGTAGAGATTTCAAATCCCTGCTACACCACAAGTGCCCTGCCAAGTTTGGAAATCTCACAACATTCTGCAGAATGTTCTGTTTTTATTTATTTTTTATTTTCTTTATTTAATGTTGGAATAGTTCTCCCCATAAAAAGTAAATAGTTTTGCTTTAGGTCTGGGATACCATGATCATCGATTCTGCCTTGAAGAGATTCTACCATTCAAATCTTTTGCTCATGATTCAAGCCATTCAAAACAACATTTCGGTATATATGCCAACTCTTTCTACTTTTATGTCCTTATGTACCATATACGATAGAAGTAAACCTGTTTTTCTTAGGTTTTGCTCTTAGTTCTTTTTCCTTATGGCTTCATCTTTCTAAATTTCTGAAGGATGAATGGCATAATGAGGTCTCAGCTTGGAGAAATTGCACAGTGAACCAAACAACTTGTCCAAACCCGTGAGTATATTCTTCTCCCTTATCCATGTTTTGTGAGATTTTTTAGATAAAGAAGAATGAAATATGAGAAATGTATATGGTGAGATGCTAATGATCAAATCATATATACAAATGGTATTCGATGATACAAACATCTTTGATCAGTATACGAAAGTTAGATGATGCTTGTGATAGAAATGAACTTGCTCCTTTCATGTCTCCAAGAAGCAGCGAAGTATAACATGTTGCTGAGACTTGAAACTGCCATTTTCTTTAGATTAGAAGGGGAGAAAAACATTCAGAGGATGAATAGTAACCTTCATTTGACTACCTACCATAAAGTTGTAGGCATAACAAAATTTTATCGGGGAGCCTCTTATTTTTCAGGTATGCTTCTGAAAGTGTTAGCATGGCATGTAAATATGCATACAAGAACGCCACTCCAGGAAGCGTACTAGAAGGTATGGTCGGAAACTTTGAATCACGTTATCTTACCAATGCTGTGATTATTATAAGTGGGGAAAATCTGATCTTTTTAACTGGGATTAGAGGTGGGCTTGGGAGTGATGCAGAAAGTGAAACCTCTTGATATCGTGGTGATGAGAAAGACAGAAAAGGGTGTAGTCCTAAGGGAATTGATCAGATAGGTCACTTGGTTTGGTGTTGCGTTTGCAATCATTTCTTCCCATAAAAAGATACACATTTGCCTGTCATAAAAATAGGATGAGACTGATAAGTTGCTTTTGTTAAATCTTCCAGTTTCTATATTCGAAATTTTAAAACCCGTCTTTCTTTATCCTTGGGAATGTCTCTGTAAGACCTCCAGTTGTGCATACGTATCTACTGAAGGGTAAGAAAGGAAGAAAGTGTAAAAACATCAGTAAAGAAAATGTTGACAACGAAAATCAGTTGGTTGGTGGGAGAGGTTTAAGAGAGGTTTTGGCTGTAGGGGAGAGGCATTGGGTGTTTTGTGATAAAAAAATTTCCTGTTTGTTGCAGACAGTTATTTTCTTAGCCGGTTACCAGTCATAGAGAAGAGGTTAGCACAAGGTGGCATTAGATTGGCTTCTACCCTCAATCGTATCTTTGCTTCTGAAGGGAAAGTGGCTGAAATTTGAAGACAGTGACTTGGAGAGATCAGATCAATTTCCATAAGATAGATGTCAAAATGGTTTCTCACATGAATTGATTTGTGCTCTTGTCTTTTGTCTTCGTATTAATATACTTGCTATTGCTCCAAAAGAACCCTAAATGTGGAAACTCATTTAGTAAAAGAACAAGATATTCATAAATAAACCAGGTCGTTTGATATAAAATCCTAACATATAGTACTTATAGTATAAACTAAAGTGATTAGATCATTGAGCCATTCATTTTTACCTTGTCTTATGAAAGAAAAAAAACCTCGAATTGAGGTGGTTAATGGGG

mRNA sequence

GTTAACTGTCCGTTTTTGTGATTCTAATTTTCGTAGCCTCTGATTTGGTGAGAGGCAAGCGTCGAAAAACGAGAAACAAGGCTGGTTTAAATTTGAATTTGGAGACAAGCTTCTAAGCCAGAGGTCAAGCAGCTGAAGTCGGAGCTGCAATCGCTGCCAGCAACTGATTTCTGATTTCATTGTGTTTATCATTACCGTATTTTGTTTCTAGGGAGCAGACATGGGTCAGTCTGAGCTTTGTTGGACTGCCAATGCTTTCCTTTTTCTCTTACTTCTACCTGGAATCCTCGGCTGGGGAAGGGAAGGTCACTATATGATTTGCAAGATAGCAGAGAAATATTTGACTGAAGATGCTCTATCAATGGTCAAAGAATTGCTTCCATCTTATGCTGAGGGCGATCTTGCAGCTGTATGCTCCTGGGCTGATGAACTTCGAGCACATCCTGATTATCACTGGAGTGGCGCCTTACACTATGTTGACACGCCAGATTTCTTTTGTAATTATAAATGCTCGAGAGACTGTCATGACAATTATAGACACAAAGGTAGATGTGTGACAGCAGCAATTTACAACTACACTATGCAACTCGAATCAGCTTACAAGGAAATAACTTCAGAAATTAAATATAACTTAACAGAGGCTCTTATGTTCTTGTCCCATTTTATCGGAGACGTTCATCAGCCCCTTCATGTCGGTTTTGTCGGAGATATAGGAGGTAATTTAATAAAAGTCAGTTGGTACCGAAGAAGGACCAATCTCCATCATGTCTGGGATACCATGATCATCGATTCTGCCTTGAAGAGATTCTACCATTCAAATCTTTTGCTCATGATTCAAGCCATTCAAAACAACATTTCGGATGAATGGCATAATGAGGTCTCAGCTTGGAGAAATTGCACAGTGAACCAAACAACTTGTCCAAACCCGTATGCTTCTGAAAGTGTTAGCATGGCATGTAAATATGCATACAAGAACGCCACTCCAGGAAGCGTACTAGAAGACAGTTATTTTCTTAGCCGGTTACCAGTCATAGAGAAGAGGTTAGCACAAGGTGGCATTAGATTGGCTTCTACCCTCAATCGTATCTTTGCTTCTGAAGGGAAAGTGGCTGAAATTTGAAGACAGTGACTTGGAGAGATCAGATCAATTTCCATAAGATAGATGTCAAAATGGTTTCTCACATGAATTGATTTGTGCTCTTGTCTTTTGTCTTCGTATTAATATACTTGCTATTGCTCCAAAAGAACCCTAAATGTGGAAACTCATTTAGTAAAAGAACAAGATATTCATAAATAAACCAGGTCGTTTGATATAAAATCCTAACATATAGTACTTATAGTATAAACTAAAGTGATTAGATCATTGAGCCATTCATTTTTACCTTGTCTTATGAAAGAAAAAAAACCTCGAATTGAGGTGGTTAATGGGG

Coding sequence (CDS)

ATGGGTCAGTCTGAGCTTTGTTGGACTGCCAATGCTTTCCTTTTTCTCTTACTTCTACCTGGAATCCTCGGCTGGGGAAGGGAAGGTCACTATATGATTTGCAAGATAGCAGAGAAATATTTGACTGAAGATGCTCTATCAATGGTCAAAGAATTGCTTCCATCTTATGCTGAGGGCGATCTTGCAGCTGTATGCTCCTGGGCTGATGAACTTCGAGCACATCCTGATTATCACTGGAGTGGCGCCTTACACTATGTTGACACGCCAGATTTCTTTTGTAATTATAAATGCTCGAGAGACTGTCATGACAATTATAGACACAAAGGTAGATGTGTGACAGCAGCAATTTACAACTACACTATGCAACTCGAATCAGCTTACAAGGAAATAACTTCAGAAATTAAATATAACTTAACAGAGGCTCTTATGTTCTTGTCCCATTTTATCGGAGACGTTCATCAGCCCCTTCATGTCGGTTTTGTCGGAGATATAGGAGGTAATTTAATAAAAGTCAGTTGGTACCGAAGAAGGACCAATCTCCATCATGTCTGGGATACCATGATCATCGATTCTGCCTTGAAGAGATTCTACCATTCAAATCTTTTGCTCATGATTCAAGCCATTCAAAACAACATTTCGGATGAATGGCATAATGAGGTCTCAGCTTGGAGAAATTGCACAGTGAACCAAACAACTTGTCCAAACCCGTATGCTTCTGAAAGTGTTAGCATGGCATGTAAATATGCATACAAGAACGCCACTCCAGGAAGCGTACTAGAAGACAGTTATTTTCTTAGCCGGTTACCAGTCATAGAGAAGAGGTTAGCACAAGGTGGCATTAGATTGGCTTCTACCCTCAATCGTATCTTTGCTTCTGAAGGGAAAGTGGCTGAAATTTGA

Protein sequence

MGQSELCWTANAFLFLLLLPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAEGDLAAVCSWADELRAHPDYHWSGALHYVDTPDFFCNYKCSRDCHDNYRHKGRCVTAAIYNYTMQLESAYKEITSEIKYNLTEALMFLSHFIGDVHQPLHVGFVGDIGGNLIKVSWYRRRTNLHHVWDTMIIDSALKRFYHSNLLLMIQAIQNNISDEWHNEVSAWRNCTVNQTTCPNPYASESVSMACKYAYKNATPGSVLEDSYFLSRLPVIEKRLAQGGIRLASTLNRIFASEGKVAEI*

Homology

[Close]

BLAST of CSPI01G14660 vs. ExPASy Swiss-Prot
Match: F4JJL0 (Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 2.3e-114
Identity = 184/297 (61.95%), Postives = 234/297 (78.79%), Query Frame = 0


Query: 1   MGQSELCWTANAFLFLLLLPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAEGD 60
           M  S   W A   +   L+ G L WG+EGHY +CKIAE Y  E+ ++ VK+LLP  A+GD
Sbjct: 1   MSSSLRQWFARVLVLTQLINGALCWGKEGHYTVCKIAESYFEEETVAAVKKLLPKSADGD 60

Query: 61  LAAVCSWADELRAHPDYHWSGALHYVDTPDFFCNYKCSRDCHDNYRHKGRCVTAAIYNYT 120
           LA+VCSW DE++ H  + W+  LHYVDTPD+ CNY+  RDCHD ++++ RCVT AI+NYT
Sbjct: 61  LASVCSWPDEIKHHWQWRWTSPLHYVDTPDYRCNYEYCRDCHDTHKNQDRCVTGAIFNYT 120

Query: 121 MQLESAYKEITSEIKYNLTEALMFLSHFIGDVHQPLHVGFVGDIGGNLIKVSWYRRRTNL 180
           MQL SA +   + + YNLTEALMFLSHFIGD+HQPLHVGF+GD GGN I V WYRR+TNL
Sbjct: 121 MQLMSASENSDTIVHYNLTEALMFLSHFIGDIHQPLHVGFLGDEGGNTITVRWYRRKTNL 180

Query: 181 HHVWDTMIIDSALKRFYHSNLLLMIQAIQNNISDEWHNEVSAWRNCTVNQTTCPNPYASE 240
           HHVWD MII+SALK +Y+ +L LMI+A+Q N++++W N+V  W +C +NQT CPNPYASE
Sbjct: 181 HHVWDNMIIESALKTYYNKSLPLMIEALQANLTNDWSNDVPLWESCQLNQTACPNPYASE 240

Query: 241 SVSMACKYAYKNATPGSVLEDSYFLSRLPVIEKRLAQGGIRLASTLNRIFASEGKVA 298
           S+++ACKYAY+NATPG+ L D YFLSRLP++EKRLAQGGIRLA+TLNRIF+S+ K A
Sbjct: 241 SINLACKYAYRNATPGTTLGDDYFLSRLPIVEKRLAQGGIRLAATLNRIFSSKPKHA 297

[Close]

BLAST of CSPI01G14660 vs. ExPASy Swiss-Prot
Match: Q8LDW6 (Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 3.9e-101
Identity = 169/297 (56.90%), Postives = 217/297 (73.06%), Query Frame = 0


Query: 1   MGQSELCWTANAFLFLLLLPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAEGD 60
           MG S   W  +  +   L+ G L WG  GHY +CKIA+ Y  ED +  VK+LLP  A G+
Sbjct: 1   MGWSLRMWIVSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGE 60

Query: 61  LAAVCSWADELRAHPDYHWSGALHYVDTPDFFCNYKCSRDCHDNYRHKGRCVTAAIYNYT 120
           LAAVCSW DE++  P + W+ ALH+ DTPD+ CNY+ SRDC  ++     CVT AI+NYT
Sbjct: 61  LAAVCSWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSRDCPKDW-----CVTGAIFNYT 120

Query: 121 MQLESAYKEITSEIKYNLTEALMFLSHFIGDVHQPLHVGFVGDIGGNLIKVSWYRRRTNL 180
            QL S  +   S + YNLTEALMFLSH++GD+HQPLH GF+GD+GGN IKV WY + TNL
Sbjct: 121 NQLMSTSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWYNQETNL 180

Query: 181 HHVWDTMIIDSALKRFYHSNLLLMIQAIQNNISDEWHNEVSAWRNCTVNQTTCPNPYASE 240
           H VWD MII+SAL+ +Y+S+L  MI  +Q  + + W N+V +W +C +NQT CPNPYASE
Sbjct: 181 HRVWDDMIIESALETYYNSSLPRMIHELQAKLKNGWSNDVPSWESCQLNQTACPNPYASE 240

Query: 241 SVSMACKYAYKNATPGSVLEDSYFLSRLPVIEKRLAQGGIRLASTLNRIFASEGKVA 298
           S+ +ACKYAY+NAT G+ L D YF+SRLPV+EKRLAQGGIRLA TLNRIF+++ K+A
Sbjct: 241 SIDLACKYAYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIFSAKRKLA 292

[Close]

BLAST of CSPI01G14660 vs. ExPASy Swiss-Prot
Match: F4JJL3 (Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.1e-100
Identity = 163/291 (56.01%), Postives = 220/291 (75.60%), Query Frame = 0


Query: 8   WTANAFLFLLLLPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAE-GDLAAVCS 67
           W  +  +   L+ G L WG++GHY +CK+AE +  +D ++ VK+LLP   + G LA  CS
Sbjct: 4   WIVSVLVLTHLVHGALCWGKDGHYTVCKLAEGFFEDDTIAAVKKLLPESVDGGGLADFCS 63

Query: 68  WADELRAHPDYHWSGALHYVDTPDFFCNYKCSRDCHDNYRHKGRCVTAAIYNYTMQLESA 127
           W DE++    + W+  LHYV+TP++ CNY+  RDCHD ++HK  CVT AI+NYT QL SA
Sbjct: 64  WPDEIKKLSQWQWTSTLHYVNTPEYRCNYEYCRDCHDTHKHKDWCVTGAIFNYTNQLMSA 123

Query: 128 YKEITSEIKYNLTEALMFLSHFIGDVHQPLHVGFVGDIGGNLIKVSWYRRRTNLHHVWDT 187
            +   + + YNLTEAL+FLSH++GDVHQPLH GF+GD+GGN I V+WY  ++NLHHVWD 
Sbjct: 124 SENSQNIVHYNLTEALLFLSHYMGDVHQPLHTGFLGDLGGNTIIVNWYHNKSNLHHVWDN 183

Query: 188 MIIDSALKRFYHSNLLLMIQAIQNNISDEWHNEVSAWRNCTVNQTTCPNPYASESVSMAC 247
           MIIDSAL+ +Y+S+L  MIQA+Q  + + W N+V +W++C  +Q  CPN YASES+ +AC
Sbjct: 184 MIIDSALETYYNSSLPHMIQALQAKLKNGWSNDVPSWKSCHFHQKACPNLYASESIDLAC 243

Query: 248 KYAYKNATPGSVLEDSYFLSRLPVIEKRLAQGGIRLASTLNRIFASEGKVA 298
           KYAY+NATPG+ L D YFLSRLPV+EKRLAQGGIRLA+TLNRIF+++ K+A
Sbjct: 244 KYAYRNATPGTTLGDEYFLSRLPVVEKRLAQGGIRLAATLNRIFSAKPKLA 294

[Close]

BLAST of CSPI01G14660 vs. ExPASy Swiss-Prot
Match: Q9C9G4 (Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 9.3e-87
Identity = 151/275 (54.91%), Postives = 194/275 (70.55%), Query Frame = 0


Query: 16  LLLLPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAEGDLAAVCSWADELRAHP 75
           L   P I GWG+EGH +ICKIA+  L E A   VKELLP  AEGDL+++C WAD ++   
Sbjct: 19  LYAAPNIHGWGKEGHEIICKIAQTRLDETAAKAVKELLPESAEGDLSSLCLWADRVKFR- 78

Query: 76  DYHWSGALHYVDTPDFFCNYKCSRDCHDNYRHKGRCVTAAIYNYTMQLESAYKEITSEIK 135
            YHWS  LHY++TPD  C+Y+ +RDC D    KGRCV  AIYNYT QL S     +S+ +
Sbjct: 79  -YHWSSPLHYINTPD-ACSYQYNRDCKDESGEKGRCVAGAIYNYTTQLLSYKTAASSQSQ 138

Query: 136 YNLTEALMFLSHFIGDVHQPLHVGFVGDIGGNLIKVSWYRRRTNLHHVWDTMIIDSALKR 195
           YNLTEAL+F+SHF+GD+HQPLHV +  D GGN I+V WY R+ NLHH+WD+ II++A   
Sbjct: 139 YNLTEALLFVSHFMGDIHQPLHVSYASDKGGNTIEVHWYTRKANLHHIWDSNIIETAEAD 198

Query: 196 FYHSNLLLMIQAIQNNISDEWHNEVSAWRNCTVNQTTCPNPYASESVSMACKYAYKNATP 255
            Y+S L  M+ A++ NI+ EW ++V  W  CT  +T CP+ YASE +  AC +AYK  T 
Sbjct: 199 LYNSALEGMVDALKKNITTEWADQVKRWETCT-KKTACPDIYASEGIQAACDWAYKGVTE 258

Query: 256 GSVLEDSYFLSRLPVIEKRLAQGGIRLASTLNRIF 291
           G  LED YF SRLP++ +RLAQGG+RLA+TLNRIF
Sbjct: 259 GDTLEDEYFYSRLPIVYQRLAQGGVRLAATLNRIF 289

[Close]

BLAST of CSPI01G14660 vs. ExPASy Swiss-Prot
Match: Q9SXA6 (Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 3.8e-80
Identity = 135/287 (47.04%), Postives = 193/287 (67.25%), Query Frame = 0


Query: 14  LFLLLLPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAEGDLAAVCSWADELRA 73
           L L  +  +  W +EGH + C+IA+  L      +V+ LLP Y +GDL+A+C W D++R 
Sbjct: 18  LILCSVSSVRSWSKEGHILTCRIAQNLLEAGPAHVVENLLPDYVKGDLSALCVWPDQIRH 77

Query: 74  HPDYHWSGALHYVDTPDFFCNYKCSRDCHDNYRHKGRCVTAAIYNYTMQLESAYKEITSE 133
              Y W+  LHY+DTPD  C+Y+ SRDCHD +  K  CV  AI N+T QL+  Y E TS+
Sbjct: 78  WYKYRWTSHLHYIDTPDQACSYEYSRDCHDQHGLKDMCVDGAIQNFTSQLQH-YGEGTSD 137

Query: 134 IKYNLTEALMFLSHFIGDVHQPLHVGFVGDIGGNLIKVSWYRRRTNLHHVWDTMIIDSAL 193
            +YN+TEAL+FLSHF+GD+HQP+HVGF  D GGN I + WY+ ++NLHHVWD  II +AL
Sbjct: 138 RRYNMTEALLFLSHFMGDIHQPMHVGFTSDEGGNTIDLRWYKHKSNLHHVWDREIILTAL 197

Query: 194 KRFYHSNLLLMIQAIQNNISDE-WHNEVSAWRNCTVNQTTCPNPYASESVSMACKYAYKN 253
           K  Y  NL L+ + ++ NI++  WH+++S+W  C  +   CP+ YASES+ +ACK+ YK 
Sbjct: 198 KENYDKNLDLLQEDLEKNITNGLWHDDLSSWTECN-DLIACPHKYASESIKLACKWGYKG 257

Query: 254 ATPGSVLEDSYFLSRLPVIEKRLAQGGIRLASTLNRIFASEGKVAEI 300
              G  L + YF +RLP++ KR+ QGG+RLA  LNR+F+ +  +A +
Sbjct: 258 VKSGETLSEEYFNTRLPIVMKRIVQGGVRLAMILNRVFSDDHAIAGV 302

[Close]

BLAST of CSPI01G14660 vs. ExPASy TrEMBL
Match: A0A0A0LY83 (Aspergillus nuclease S(1) OS=Cucumis sativus OX=3659 GN=Csa_1G163110 PE=3 SV=1)

HSP 1 Score: 630.2 bits (1624), Expect = 4.5e-177
Identity = 298/299 (99.67%), Postives = 298/299 (99.67%), Query Frame = 0


Query: 1   MGQSELCWTANAFLFLLLLPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAEGD 60
           MGQSELCWTANAFLFLL LPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAEGD
Sbjct: 1   MGQSELCWTANAFLFLLFLPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAEGD 60

Query: 61  LAAVCSWADELRAHPDYHWSGALHYVDTPDFFCNYKCSRDCHDNYRHKGRCVTAAIYNYT 120
           LAAVCSWADELRAHPDYHWSGALHYVDTPDFFCNYKCSRDCHDNYRHKGRCVTAAIYNYT
Sbjct: 61  LAAVCSWADELRAHPDYHWSGALHYVDTPDFFCNYKCSRDCHDNYRHKGRCVTAAIYNYT 120

Query: 121 MQLESAYKEITSEIKYNLTEALMFLSHFIGDVHQPLHVGFVGDIGGNLIKVSWYRRRTNL 180
           MQLESAYKEITSEIKYNLTEALMFLSHFIGDVHQPLHVGFVGDIGGNLIKVSWYRRRTNL
Sbjct: 121 MQLESAYKEITSEIKYNLTEALMFLSHFIGDVHQPLHVGFVGDIGGNLIKVSWYRRRTNL 180

Query: 181 HHVWDTMIIDSALKRFYHSNLLLMIQAIQNNISDEWHNEVSAWRNCTVNQTTCPNPYASE 240
           HHVWDTMIIDSALKRFYHSNLLLMIQAIQNNISDEWHNEVSAWRNCTVNQTTCPNPYASE
Sbjct: 181 HHVWDTMIIDSALKRFYHSNLLLMIQAIQNNISDEWHNEVSAWRNCTVNQTTCPNPYASE 240

Query: 241 SVSMACKYAYKNATPGSVLEDSYFLSRLPVIEKRLAQGGIRLASTLNRIFASEGKVAEI 300
           SVSMACKYAYKNATPGSVLEDSYFLSRLPVIEKRLAQGGIRLASTLNRIFASEGKVAEI
Sbjct: 241 SVSMACKYAYKNATPGSVLEDSYFLSRLPVIEKRLAQGGIRLASTLNRIFASEGKVAEI 299

[Close]

BLAST of CSPI01G14660 vs. ExPASy TrEMBL
Match: A0A1S3CEU3 (Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103500060 PE=3 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 9.4e-159
Identity = 262/299 (87.63%), Postives = 282/299 (94.31%), Query Frame = 0


Query: 1   MGQSELCWTANAFLFLLLLPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAEGD 60
           MGQSELCWTAN F+FLLLLPGILGWGREGHY +CKIAE YLTEDALSMV+ELLP+ AEGD
Sbjct: 21  MGQSELCWTANVFVFLLLLPGILGWGREGHYAVCKIAEGYLTEDALSMVRELLPASAEGD 80

Query: 61  LAAVCSWADELRAHPDYHWSGALHYVDTPDFFCNYKCSRDCHDNYRHKGRCVTAAIYNYT 120
           LAAVCSWADELR + DYHWS  LH+VDTPDFFCNY CSRDCHD++RHKGRCVTAAIYNYT
Sbjct: 81  LAAVCSWADELRENHDYHWSSVLHFVDTPDFFCNYNCSRDCHDSHRHKGRCVTAAIYNYT 140

Query: 121 MQLESAYKEITSEIKYNLTEALMFLSHFIGDVHQPLHVGFVGDIGGNLIKVSWYRRRTNL 180
           MQLESAYKEITSE++YNLTEALMFLSHFIGDVHQPLHVGFVGD+GGNLIKV WY+R TNL
Sbjct: 141 MQLESAYKEITSEVRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNLIKVGWYQRSTNL 200

Query: 181 HHVWDTMIIDSALKRFYHSNLLLMIQAIQNNISDEWHNEVSAWRNCTVNQTTCPNPYASE 240
           HHVWDTMII+SALKRFYHSNL+LMIQAIQ+NISDEWHNEVSAWRNCT N T CPNPYASE
Sbjct: 201 HHVWDTMIIESALKRFYHSNLMLMIQAIQSNISDEWHNEVSAWRNCTGNHTACPNPYASE 260

Query: 241 SVSMACKYAYKNATPGSVLEDSYFLSRLPVIEKRLAQGGIRLASTLNRIFASEGKVAEI 300
           S+S+ACKYAYKNATPGS LEDSYFL+RLPV+EKRLAQ GIRLASTLNRIFASE KVAE+
Sbjct: 261 SISLACKYAYKNATPGSTLEDSYFLTRLPVVEKRLAQSGIRLASTLNRIFASEVKVAEV 319

[Close]

BLAST of CSPI01G14660 vs. ExPASy TrEMBL
Match: A0A1S3CF84 (Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103500060 PE=3 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 9.4e-159
Identity = 262/299 (87.63%), Postives = 282/299 (94.31%), Query Frame = 0


Query: 1   MGQSELCWTANAFLFLLLLPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAEGD 60
           MGQSELCWTAN F+FLLLLPGILGWGREGHY +CKIAE YLTEDALSMV+ELLP+ AEGD
Sbjct: 1   MGQSELCWTANVFVFLLLLPGILGWGREGHYAVCKIAEGYLTEDALSMVRELLPASAEGD 60

Query: 61  LAAVCSWADELRAHPDYHWSGALHYVDTPDFFCNYKCSRDCHDNYRHKGRCVTAAIYNYT 120
           LAAVCSWADELR + DYHWS  LH+VDTPDFFCNY CSRDCHD++RHKGRCVTAAIYNYT
Sbjct: 61  LAAVCSWADELRENHDYHWSSVLHFVDTPDFFCNYNCSRDCHDSHRHKGRCVTAAIYNYT 120

Query: 121 MQLESAYKEITSEIKYNLTEALMFLSHFIGDVHQPLHVGFVGDIGGNLIKVSWYRRRTNL 180
           MQLESAYKEITSE++YNLTEALMFLSHFIGDVHQPLHVGFVGD+GGNLIKV WY+R TNL
Sbjct: 121 MQLESAYKEITSEVRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNLIKVGWYQRSTNL 180

Query: 181 HHVWDTMIIDSALKRFYHSNLLLMIQAIQNNISDEWHNEVSAWRNCTVNQTTCPNPYASE 240
           HHVWDTMII+SALKRFYHSNL+LMIQAIQ+NISDEWHNEVSAWRNCT N T CPNPYASE
Sbjct: 181 HHVWDTMIIESALKRFYHSNLMLMIQAIQSNISDEWHNEVSAWRNCTGNHTACPNPYASE 240

Query: 241 SVSMACKYAYKNATPGSVLEDSYFLSRLPVIEKRLAQGGIRLASTLNRIFASEGKVAEI 300
           S+S+ACKYAYKNATPGS LEDSYFL+RLPV+EKRLAQ GIRLASTLNRIFASE KVAE+
Sbjct: 241 SISLACKYAYKNATPGSTLEDSYFLTRLPVVEKRLAQSGIRLASTLNRIFASEVKVAEV 299

[Close]

BLAST of CSPI01G14660 vs. ExPASy TrEMBL
Match: A0A6J1IVA4 (Aspergillus nuclease S(1) OS=Cucurbita maxima OX=3661 GN=LOC111478715 PE=3 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 2.4e-146
Identity = 247/299 (82.61%), Postives = 265/299 (88.63%), Query Frame = 0


Query: 1   MGQSELCWTANAFLFLLLLPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAEGD 60
           MGQSELC  A AF FLLL+PGILGWG+EGHY+ICKIAE Y TED LSMVKELLP+ AEGD
Sbjct: 1   MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60

Query: 61  LAAVCSWADELRAHPDYHWSGALHYVDTPDFFCNYKCSRDCHDNYRHKGRCVTAAIYNYT 120
           LAAVCSW DE+R    Y WS ALHYVDTPDFFCNY  SRDCHD + HKGRCVTAAIYNYT
Sbjct: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHEHKGRCVTAAIYNYT 120

Query: 121 MQLESAYKEITSEIKYNLTEALMFLSHFIGDVHQPLHVGFVGDIGGNLIKVSWYRRRTNL 180
            QLESAYKE+TSEI+YNLTEALMFLSHFIGDVHQPLHVGFVGD+GGN I V WYRR+TNL
Sbjct: 121 RQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180

Query: 181 HHVWDTMIIDSALKRFYHSNLLLMIQAIQNNISDEWHNEVSAWRNCTVNQTTCPNPYASE 240
           HHVWD MII+SALK FYHSNL LMIQAIQ NIS EW+N+VSAWRNC +N T CPNPYASE
Sbjct: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQTNISGEWYNDVSAWRNCALNHTACPNPYASE 240

Query: 241 SVSMACKYAYKNATPGSVLEDSYFLSRLPVIEKRLAQGGIRLASTLNRIFASEGKVAEI 300
           S+S+ACKYAYKNATPGS LEDSYFLSRLP++EKRLAQ GIRLASTLNRIFASE KVAEI
Sbjct: 241 SISLACKYAYKNATPGSTLEDSYFLSRLPIVEKRLAQSGIRLASTLNRIFASEVKVAEI 299

[Close]

BLAST of CSPI01G14660 vs. ExPASy TrEMBL
Match: A0A6J1GEX6 (Aspergillus nuclease S(1) OS=Cucurbita moschata OX=3662 GN=LOC111453339 PE=3 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 1.7e-144
Identity = 244/299 (81.61%), Postives = 263/299 (87.96%), Query Frame = 0


Query: 1   MGQSELCWTANAFLFLLLLPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAEGD 60
           MGQ ELC  A AF FLLL+PGILGWG+EGHY+ICKIAE Y TED LSMVKELLP+ AEGD
Sbjct: 1   MGQFELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60

Query: 61  LAAVCSWADELRAHPDYHWSGALHYVDTPDFFCNYKCSRDCHDNYRHKGRCVTAAIYNYT 120
           LAAVCSW DE+R    Y WS ALHYVDTPDFFCNY  SRDCHD + HKGRCVTAAIYNYT
Sbjct: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYT 120

Query: 121 MQLESAYKEITSEIKYNLTEALMFLSHFIGDVHQPLHVGFVGDIGGNLIKVSWYRRRTNL 180
            QLESAYKE+TSEI+YNLTEALMFLSHFIGDVHQPLHVGFVGD+GGN I V WYRR+TNL
Sbjct: 121 RQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180

Query: 181 HHVWDTMIIDSALKRFYHSNLLLMIQAIQNNISDEWHNEVSAWRNCTVNQTTCPNPYASE 240
           HHVWD MII+SALK FYHSNL LMIQAIQ NIS EW+N+VSAWRNC +N T CPNPYASE
Sbjct: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQTNISGEWYNDVSAWRNCALNHTACPNPYASE 240

Query: 241 SVSMACKYAYKNATPGSVLEDSYFLSRLPVIEKRLAQGGIRLASTLNRIFASEGKVAEI 300
           S+ +ACKYAY+NATPGS LEDSYFLSRLP++EKRLAQ GIRLASTLNRIFASE KVAEI
Sbjct: 241 SIRLACKYAYRNATPGSTLEDSYFLSRLPIVEKRLAQSGIRLASTLNRIFASEVKVAEI 299

[Close]

BLAST of CSPI01G14660 vs. NCBI nr
Match: XP_004139483.1 (endonuclease 4 isoform X1 [Cucumis sativus])

HSP 1 Score: 630.2 bits (1624), Expect = 9.3e-177
Identity = 298/299 (99.67%), Postives = 298/299 (99.67%), Query Frame = 0


Query: 1   MGQSELCWTANAFLFLLLLPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAEGD 60
           MGQSELCWTANAFLFLL LPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAEGD
Sbjct: 1   MGQSELCWTANAFLFLLFLPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAEGD 60

Query: 61  LAAVCSWADELRAHPDYHWSGALHYVDTPDFFCNYKCSRDCHDNYRHKGRCVTAAIYNYT 120
           LAAVCSWADELRAHPDYHWSGALHYVDTPDFFCNYKCSRDCHDNYRHKGRCVTAAIYNYT
Sbjct: 61  LAAVCSWADELRAHPDYHWSGALHYVDTPDFFCNYKCSRDCHDNYRHKGRCVTAAIYNYT 120

Query: 121 MQLESAYKEITSEIKYNLTEALMFLSHFIGDVHQPLHVGFVGDIGGNLIKVSWYRRRTNL 180
           MQLESAYKEITSEIKYNLTEALMFLSHFIGDVHQPLHVGFVGDIGGNLIKVSWYRRRTNL
Sbjct: 121 MQLESAYKEITSEIKYNLTEALMFLSHFIGDVHQPLHVGFVGDIGGNLIKVSWYRRRTNL 180

Query: 181 HHVWDTMIIDSALKRFYHSNLLLMIQAIQNNISDEWHNEVSAWRNCTVNQTTCPNPYASE 240
           HHVWDTMIIDSALKRFYHSNLLLMIQAIQNNISDEWHNEVSAWRNCTVNQTTCPNPYASE
Sbjct: 181 HHVWDTMIIDSALKRFYHSNLLLMIQAIQNNISDEWHNEVSAWRNCTVNQTTCPNPYASE 240

Query: 241 SVSMACKYAYKNATPGSVLEDSYFLSRLPVIEKRLAQGGIRLASTLNRIFASEGKVAEI 300
           SVSMACKYAYKNATPGSVLEDSYFLSRLPVIEKRLAQGGIRLASTLNRIFASEGKVAEI
Sbjct: 241 SVSMACKYAYKNATPGSVLEDSYFLSRLPVIEKRLAQGGIRLASTLNRIFASEGKVAEI 299

[Close]

BLAST of CSPI01G14660 vs. NCBI nr
Match: XP_008461475.1 (PREDICTED: endonuclease 4-like isoform X1 [Cucumis melo])

HSP 1 Score: 569.3 bits (1466), Expect = 1.9e-158
Identity = 262/299 (87.63%), Postives = 282/299 (94.31%), Query Frame = 0


Query: 1   MGQSELCWTANAFLFLLLLPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAEGD 60
           MGQSELCWTAN F+FLLLLPGILGWGREGHY +CKIAE YLTEDALSMV+ELLP+ AEGD
Sbjct: 21  MGQSELCWTANVFVFLLLLPGILGWGREGHYAVCKIAEGYLTEDALSMVRELLPASAEGD 80

Query: 61  LAAVCSWADELRAHPDYHWSGALHYVDTPDFFCNYKCSRDCHDNYRHKGRCVTAAIYNYT 120
           LAAVCSWADELR + DYHWS  LH+VDTPDFFCNY CSRDCHD++RHKGRCVTAAIYNYT
Sbjct: 81  LAAVCSWADELRENHDYHWSSVLHFVDTPDFFCNYNCSRDCHDSHRHKGRCVTAAIYNYT 140

Query: 121 MQLESAYKEITSEIKYNLTEALMFLSHFIGDVHQPLHVGFVGDIGGNLIKVSWYRRRTNL 180
           MQLESAYKEITSE++YNLTEALMFLSHFIGDVHQPLHVGFVGD+GGNLIKV WY+R TNL
Sbjct: 141 MQLESAYKEITSEVRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNLIKVGWYQRSTNL 200

Query: 181 HHVWDTMIIDSALKRFYHSNLLLMIQAIQNNISDEWHNEVSAWRNCTVNQTTCPNPYASE 240
           HHVWDTMII+SALKRFYHSNL+LMIQAIQ+NISDEWHNEVSAWRNCT N T CPNPYASE
Sbjct: 201 HHVWDTMIIESALKRFYHSNLMLMIQAIQSNISDEWHNEVSAWRNCTGNHTACPNPYASE 260

Query: 241 SVSMACKYAYKNATPGSVLEDSYFLSRLPVIEKRLAQGGIRLASTLNRIFASEGKVAEI 300
           S+S+ACKYAYKNATPGS LEDSYFL+RLPV+EKRLAQ GIRLASTLNRIFASE KVAE+
Sbjct: 261 SISLACKYAYKNATPGSTLEDSYFLTRLPVVEKRLAQSGIRLASTLNRIFASEVKVAEV 319

[Close]

BLAST of CSPI01G14660 vs. NCBI nr
Match: XP_008461483.1 (PREDICTED: endonuclease 4-like isoform X2 [Cucumis melo])

HSP 1 Score: 569.3 bits (1466), Expect = 1.9e-158
Identity = 262/299 (87.63%), Postives = 282/299 (94.31%), Query Frame = 0


Query: 1   MGQSELCWTANAFLFLLLLPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAEGD 60
           MGQSELCWTAN F+FLLLLPGILGWGREGHY +CKIAE YLTEDALSMV+ELLP+ AEGD
Sbjct: 1   MGQSELCWTANVFVFLLLLPGILGWGREGHYAVCKIAEGYLTEDALSMVRELLPASAEGD 60

Query: 61  LAAVCSWADELRAHPDYHWSGALHYVDTPDFFCNYKCSRDCHDNYRHKGRCVTAAIYNYT 120
           LAAVCSWADELR + DYHWS  LH+VDTPDFFCNY CSRDCHD++RHKGRCVTAAIYNYT
Sbjct: 61  LAAVCSWADELRENHDYHWSSVLHFVDTPDFFCNYNCSRDCHDSHRHKGRCVTAAIYNYT 120

Query: 121 MQLESAYKEITSEIKYNLTEALMFLSHFIGDVHQPLHVGFVGDIGGNLIKVSWYRRRTNL 180
           MQLESAYKEITSE++YNLTEALMFLSHFIGDVHQPLHVGFVGD+GGNLIKV WY+R TNL
Sbjct: 121 MQLESAYKEITSEVRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNLIKVGWYQRSTNL 180

Query: 181 HHVWDTMIIDSALKRFYHSNLLLMIQAIQNNISDEWHNEVSAWRNCTVNQTTCPNPYASE 240
           HHVWDTMII+SALKRFYHSNL+LMIQAIQ+NISDEWHNEVSAWRNCT N T CPNPYASE
Sbjct: 181 HHVWDTMIIESALKRFYHSNLMLMIQAIQSNISDEWHNEVSAWRNCTGNHTACPNPYASE 240

Query: 241 SVSMACKYAYKNATPGSVLEDSYFLSRLPVIEKRLAQGGIRLASTLNRIFASEGKVAEI 300
           S+S+ACKYAYKNATPGS LEDSYFL+RLPV+EKRLAQ GIRLASTLNRIFASE KVAE+
Sbjct: 241 SISLACKYAYKNATPGSTLEDSYFLTRLPVVEKRLAQSGIRLASTLNRIFASEVKVAEV 299

[Close]

BLAST of CSPI01G14660 vs. NCBI nr
Match: XP_038883252.1 (endonuclease 4-like isoform X2 [Benincasa hispida])

HSP 1 Score: 547.7 bits (1410), Expect = 6.1e-152
Identity = 253/299 (84.62%), Postives = 273/299 (91.30%), Query Frame = 0


Query: 1   MGQSELCWTANAFLFLLLLPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAEGD 60
           MGQS+LCWTANAF+FLLLLPGILGWG+EGHY+ICKIAE Y TED LS VKELLP+ AEGD
Sbjct: 1   MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGD 60

Query: 61  LAAVCSWADELRAHPDYHWSGALHYVDTPDFFCNYKCSRDCHDNYRHKGRCVTAAIYNYT 120
           LAAVCSW DE++    Y WS ALHYVDTPDFFCNY CSRDCHD + HKGRCVTAAIYNYT
Sbjct: 61  LAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSRDCHDTHGHKGRCVTAAIYNYT 120

Query: 121 MQLESAYKEITSEIKYNLTEALMFLSHFIGDVHQPLHVGFVGDIGGNLIKVSWYRRRTNL 180
           MQLESAYKE+TSEI+YNLTEALMFLSHFIGDVHQPLHVGFVGD+GGN I V WYRR+TNL
Sbjct: 121 MQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180

Query: 181 HHVWDTMIIDSALKRFYHSNLLLMIQAIQNNISDEWHNEVSAWRNCTVNQTTCPNPYASE 240
           HHVWD MII+SALK FYHSNL LMIQAIQNNISDEW+N+VSAWRNCT+N T CPNPYASE
Sbjct: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACPNPYASE 240

Query: 241 SVSMACKYAYKNATPGSVLEDSYFLSRLPVIEKRLAQGGIRLASTLNRIFASEGKVAEI 300
           S+S+ACKYAYKNATPGS LEDSYFLSRLPV+EKRLAQ GIRLASTLNRIFASE KVAE+
Sbjct: 241 SISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEVKVAEV 299

[Close]

BLAST of CSPI01G14660 vs. NCBI nr
Match: XP_038883248.1 (endonuclease 4-like isoform X1 [Benincasa hispida] >XP_038883249.1 endonuclease 4-like isoform X1 [Benincasa hispida] >XP_038883250.1 endonuclease 4-like isoform X1 [Benincasa hispida] >XP_038883251.1 endonuclease 4-like isoform X1 [Benincasa hispida])

HSP 1 Score: 538.9 bits (1387), Expect = 2.8e-149
Identity = 252/305 (82.62%), Postives = 272/305 (89.18%), Query Frame = 0


Query: 1   MGQSELCWTANAFLFLLLLPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAEGD 60
           MGQS+LCWTANAF+FLLLLPGILGWG+EGHY+ICKIAE Y TED LS VKELLP+ AEGD
Sbjct: 1   MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGD 60

Query: 61  LAAVCSWADELRAHPDYHWSGALHYVDTPDFFCNYKCSR------DCHDNYRHKGRCVTA 120
           LAAVCSW DE++    Y WS ALHYVDTPDFFCNY CS       DCHD + HKGRCVTA
Sbjct: 61  LAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSSKLKLYGDCHDTHGHKGRCVTA 120

Query: 121 AIYNYTMQLESAYKEITSEIKYNLTEALMFLSHFIGDVHQPLHVGFVGDIGGNLIKVSWY 180
           AIYNYTMQLESAYKE+TSEI+YNLTEALMFLSHFIGDVHQPLHVGFVGD+GGN I V WY
Sbjct: 121 AIYNYTMQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWY 180

Query: 181 RRRTNLHHVWDTMIIDSALKRFYHSNLLLMIQAIQNNISDEWHNEVSAWRNCTVNQTTCP 240
           RR+TNLHHVWD MII+SALK FYHSNL LMIQAIQNNISDEW+N+VSAWRNCT+N T CP
Sbjct: 181 RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACP 240

Query: 241 NPYASESVSMACKYAYKNATPGSVLEDSYFLSRLPVIEKRLAQGGIRLASTLNRIFASEG 300
           NPYASES+S+ACKYAYKNATPGS LEDSYFLSRLPV+EKRLAQ GIRLASTLNRIFASE 
Sbjct: 241 NPYASESISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEV 300

[Close]

BLAST of CSPI01G14660 vs. TAIR 10
Match: AT4G21585.1 (endonuclease 4 )

HSP 1 Score: 413.3 bits (1061), Expect = 1.7e-115
Identity = 184/297 (61.95%), Postives = 234/297 (78.79%), Query Frame = 0


Query: 1   MGQSELCWTANAFLFLLLLPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAEGD 60
           M  S   W A   +   L+ G L WG+EGHY +CKIAE Y  E+ ++ VK+LLP  A+GD
Sbjct: 1   MSSSLRQWFARVLVLTQLINGALCWGKEGHYTVCKIAESYFEEETVAAVKKLLPKSADGD 60

Query: 61  LAAVCSWADELRAHPDYHWSGALHYVDTPDFFCNYKCSRDCHDNYRHKGRCVTAAIYNYT 120
           LA+VCSW DE++ H  + W+  LHYVDTPD+ CNY+  RDCHD ++++ RCVT AI+NYT
Sbjct: 61  LASVCSWPDEIKHHWQWRWTSPLHYVDTPDYRCNYEYCRDCHDTHKNQDRCVTGAIFNYT 120

Query: 121 MQLESAYKEITSEIKYNLTEALMFLSHFIGDVHQPLHVGFVGDIGGNLIKVSWYRRRTNL 180
           MQL SA +   + + YNLTEALMFLSHFIGD+HQPLHVGF+GD GGN I V WYRR+TNL
Sbjct: 121 MQLMSASENSDTIVHYNLTEALMFLSHFIGDIHQPLHVGFLGDEGGNTITVRWYRRKTNL 180

Query: 181 HHVWDTMIIDSALKRFYHSNLLLMIQAIQNNISDEWHNEVSAWRNCTVNQTTCPNPYASE 240
           HHVWD MII+SALK +Y+ +L LMI+A+Q N++++W N+V  W +C +NQT CPNPYASE
Sbjct: 181 HHVWDNMIIESALKTYYNKSLPLMIEALQANLTNDWSNDVPLWESCQLNQTACPNPYASE 240

Query: 241 SVSMACKYAYKNATPGSVLEDSYFLSRLPVIEKRLAQGGIRLASTLNRIFASEGKVA 298
           S+++ACKYAY+NATPG+ L D YFLSRLP++EKRLAQGGIRLA+TLNRIF+S+ K A
Sbjct: 241 SINLACKYAYRNATPGTTLGDDYFLSRLPIVEKRLAQGGIRLAATLNRIFSSKPKHA 297

[Close]

BLAST of CSPI01G14660 vs. TAIR 10
Match: AT4G21590.1 (endonuclease 3 )

HSP 1 Score: 369.4 bits (947), Expect = 2.8e-102
Identity = 169/297 (56.90%), Postives = 217/297 (73.06%), Query Frame = 0


Query: 1   MGQSELCWTANAFLFLLLLPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAEGD 60
           MG S   W  +  +   L+ G L WG  GHY +CKIA+ Y  ED +  VK+LLP  A G+
Sbjct: 1   MGWSLRMWIVSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGE 60

Query: 61  LAAVCSWADELRAHPDYHWSGALHYVDTPDFFCNYKCSRDCHDNYRHKGRCVTAAIYNYT 120
           LAAVCSW DE++  P + W+ ALH+ DTPD+ CNY+ SRDC  ++     CVT AI+NYT
Sbjct: 61  LAAVCSWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSRDCPKDW-----CVTGAIFNYT 120

Query: 121 MQLESAYKEITSEIKYNLTEALMFLSHFIGDVHQPLHVGFVGDIGGNLIKVSWYRRRTNL 180
            QL S  +   S + YNLTEALMFLSH++GD+HQPLH GF+GD+GGN IKV WY + TNL
Sbjct: 121 NQLMSTSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWYNQETNL 180

Query: 181 HHVWDTMIIDSALKRFYHSNLLLMIQAIQNNISDEWHNEVSAWRNCTVNQTTCPNPYASE 240
           H VWD MII+SAL+ +Y+S+L  MI  +Q  + + W N+V +W +C +NQT CPNPYASE
Sbjct: 181 HRVWDDMIIESALETYYNSSLPRMIHELQAKLKNGWSNDVPSWESCQLNQTACPNPYASE 240

Query: 241 SVSMACKYAYKNATPGSVLEDSYFLSRLPVIEKRLAQGGIRLASTLNRIFASEGKVA 298
           S+ +ACKYAY+NAT G+ L D YF+SRLPV+EKRLAQGGIRLA TLNRIF+++ K+A
Sbjct: 241 SIDLACKYAYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIFSAKRKLA 292

[Close]

BLAST of CSPI01G14660 vs. TAIR 10
Match: AT4G21590.2 (endonuclease 3 )

HSP 1 Score: 369.4 bits (947), Expect = 2.8e-102
Identity = 169/297 (56.90%), Postives = 217/297 (73.06%), Query Frame = 0


Query: 1   MGQSELCWTANAFLFLLLLPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAEGD 60
           MG S   W  +  +   L+ G L WG  GHY +CKIA+ Y  ED +  VK+LLP  A G+
Sbjct: 1   MGWSLRMWIVSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGE 60

Query: 61  LAAVCSWADELRAHPDYHWSGALHYVDTPDFFCNYKCSRDCHDNYRHKGRCVTAAIYNYT 120
           LAAVCSW DE++  P + W+ ALH+ DTPD+ CNY+ SRDC  ++     CVT AI+NYT
Sbjct: 61  LAAVCSWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSRDCPKDW-----CVTGAIFNYT 120

Query: 121 MQLESAYKEITSEIKYNLTEALMFLSHFIGDVHQPLHVGFVGDIGGNLIKVSWYRRRTNL 180
            QL S  +   S + YNLTEALMFLSH++GD+HQPLH GF+GD+GGN IKV WY + TNL
Sbjct: 121 NQLMSTSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWYNQETNL 180

Query: 181 HHVWDTMIIDSALKRFYHSNLLLMIQAIQNNISDEWHNEVSAWRNCTVNQTTCPNPYASE 240
           H VWD MII+SAL+ +Y+S+L  MI  +Q  + + W N+V +W +C +NQT CPNPYASE
Sbjct: 181 HRVWDDMIIESALETYYNSSLPRMIHELQAKLKNGWSNDVPSWESCQLNQTACPNPYASE 240

Query: 241 SVSMACKYAYKNATPGSVLEDSYFLSRLPVIEKRLAQGGIRLASTLNRIFASEGKVA 298
           S+ +ACKYAY+NAT G+ L D YF+SRLPV+EKRLAQGGIRLA TLNRIF+++ K+A
Sbjct: 241 SIDLACKYAYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIFSAKRKLA 292

[Close]

BLAST of CSPI01G14660 vs. TAIR 10
Match: AT4G21600.1 (endonuclease 5 )

HSP 1 Score: 367.9 bits (943), Expect = 8.0e-102
Identity = 163/291 (56.01%), Postives = 220/291 (75.60%), Query Frame = 0


Query: 8   WTANAFLFLLLLPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAE-GDLAAVCS 67
           W  +  +   L+ G L WG++GHY +CK+AE +  +D ++ VK+LLP   + G LA  CS
Sbjct: 4   WIVSVLVLTHLVHGALCWGKDGHYTVCKLAEGFFEDDTIAAVKKLLPESVDGGGLADFCS 63

Query: 68  WADELRAHPDYHWSGALHYVDTPDFFCNYKCSRDCHDNYRHKGRCVTAAIYNYTMQLESA 127
           W DE++    + W+  LHYV+TP++ CNY+  RDCHD ++HK  CVT AI+NYT QL SA
Sbjct: 64  WPDEIKKLSQWQWTSTLHYVNTPEYRCNYEYCRDCHDTHKHKDWCVTGAIFNYTNQLMSA 123

Query: 128 YKEITSEIKYNLTEALMFLSHFIGDVHQPLHVGFVGDIGGNLIKVSWYRRRTNLHHVWDT 187
            +   + + YNLTEAL+FLSH++GDVHQPLH GF+GD+GGN I V+WY  ++NLHHVWD 
Sbjct: 124 SENSQNIVHYNLTEALLFLSHYMGDVHQPLHTGFLGDLGGNTIIVNWYHNKSNLHHVWDN 183

Query: 188 MIIDSALKRFYHSNLLLMIQAIQNNISDEWHNEVSAWRNCTVNQTTCPNPYASESVSMAC 247
           MIIDSAL+ +Y+S+L  MIQA+Q  + + W N+V +W++C  +Q  CPN YASES+ +AC
Sbjct: 184 MIIDSALETYYNSSLPHMIQALQAKLKNGWSNDVPSWKSCHFHQKACPNLYASESIDLAC 243

Query: 248 KYAYKNATPGSVLEDSYFLSRLPVIEKRLAQGGIRLASTLNRIFASEGKVA 298
           KYAY+NATPG+ L D YFLSRLPV+EKRLAQGGIRLA+TLNRIF+++ K+A
Sbjct: 244 KYAYRNATPGTTLGDEYFLSRLPVVEKRLAQGGIRLAATLNRIFSAKPKLA 294

[Close]

BLAST of CSPI01G14660 vs. TAIR 10
Match: AT1G68290.1 (endonuclease 2 )

HSP 1 Score: 321.6 bits (823), Expect = 6.6e-88
Identity = 151/275 (54.91%), Postives = 194/275 (70.55%), Query Frame = 0


Query: 16  LLLLPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAEGDLAAVCSWADELRAHP 75
           L   P I GWG+EGH +ICKIA+  L E A   VKELLP  AEGDL+++C WAD ++   
Sbjct: 19  LYAAPNIHGWGKEGHEIICKIAQTRLDETAAKAVKELLPESAEGDLSSLCLWADRVKFR- 78

Query: 76  DYHWSGALHYVDTPDFFCNYKCSRDCHDNYRHKGRCVTAAIYNYTMQLESAYKEITSEIK 135
            YHWS  LHY++TPD  C+Y+ +RDC D    KGRCV  AIYNYT QL S     +S+ +
Sbjct: 79  -YHWSSPLHYINTPD-ACSYQYNRDCKDESGEKGRCVAGAIYNYTTQLLSYKTAASSQSQ 138

Query: 136 YNLTEALMFLSHFIGDVHQPLHVGFVGDIGGNLIKVSWYRRRTNLHHVWDTMIIDSALKR 195
           YNLTEAL+F+SHF+GD+HQPLHV +  D GGN I+V WY R+ NLHH+WD+ II++A   
Sbjct: 139 YNLTEALLFVSHFMGDIHQPLHVSYASDKGGNTIEVHWYTRKANLHHIWDSNIIETAEAD 198

Query: 196 FYHSNLLLMIQAIQNNISDEWHNEVSAWRNCTVNQTTCPNPYASESVSMACKYAYKNATP 255
            Y+S L  M+ A++ NI+ EW ++V  W  CT  +T CP+ YASE +  AC +AYK  T 
Sbjct: 199 LYNSALEGMVDALKKNITTEWADQVKRWETCT-KKTACPDIYASEGIQAACDWAYKGVTE 258

Query: 256 GSVLEDSYFLSRLPVIEKRLAQGGIRLASTLNRIF 291
           G  LED YF SRLP++ +RLAQGG+RLA+TLNRIF
Sbjct: 259 GDTLEDEYFYSRLPIVYQRLAQGGVRLAATLNRIF 289

The following BLAST results are available for this feature:

BLAST of CSPI01G14660 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JJL0	2.3e-114	61.95	Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1	[more]
Q8LDW6	3.9e-101	56.90	Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1	[more]
F4JJL3	1.1e-100	56.01	Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1	[more]
Q9C9G4	9.3e-87	54.91	Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1	[more]
Q9SXA6	3.8e-80	47.04	Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1	[more]

BLAST of CSPI01G14660 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LY83	4.5e-177	99.67	Aspergillus nuclease S(1) OS=Cucumis sativus OX=3659 GN=Csa_1G163110 PE=3 SV=1	[more]
A0A1S3CEU3	9.4e-159	87.63	Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103500060 PE=3 SV=1	[more]
A0A1S3CF84	9.4e-159	87.63	Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103500060 PE=3 SV=1	[more]
A0A6J1IVA4	2.4e-146	82.61	Aspergillus nuclease S(1) OS=Cucurbita maxima OX=3661 GN=LOC111478715 PE=3 SV=1	[more]
A0A6J1GEX6	1.7e-144	81.61	Aspergillus nuclease S(1) OS=Cucurbita moschata OX=3662 GN=LOC111453339 PE=3 SV=...	[more]

BLAST of CSPI01G14660 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004139483.1	9.3e-177	99.67	endonuclease 4 isoform X1 [Cucumis sativus]	[more]
XP_008461475.1	1.9e-158	87.63	PREDICTED: endonuclease 4-like isoform X1 [Cucumis melo]	[more]
XP_008461483.1	1.9e-158	87.63	PREDICTED: endonuclease 4-like isoform X2 [Cucumis melo]	[more]
XP_038883252.1	6.1e-152	84.62	endonuclease 4-like isoform X2 [Benincasa hispida]	[more]
XP_038883248.1	2.8e-149	82.62	endonuclease 4-like isoform X1 [Benincasa hispida] >XP_038883249.1 endonuclease ...	[more]

BLAST of CSPI01G14660 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G21585.1	1.7e-115	61.95	endonuclease 4	[more]
AT4G21590.1	2.8e-102	56.90	endonuclease 3	[more]
AT4G21590.2	2.8e-102	56.90	endonuclease 3	[more]
AT4G21600.1	8.0e-102	56.01	endonuclease 5	[more]
AT1G68290.1	6.6e-88	54.91	endonuclease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003154	S1/P1 nuclease	PFAM	PF02265	S1-P1_nuclease	coord: 25..290 e-value: 6.4E-78 score: 262.1
IPR003154	S1/P1 nuclease	PANTHER	PTHR33146	ENDONUCLEASE 4	coord: 10..293
IPR003154	S1/P1 nuclease	CDD	cd11010	S1-P1_nuclease	coord: 25..290 e-value: 8.65262E-85 score: 253.076
IPR008947	Phospholipase C/P1 nuclease domain superfamily	GENE3D	1.10.575.10	P1 Nuclease	coord: 25..295 e-value: 3.1E-99 score: 334.1
IPR008947	Phospholipase C/P1 nuclease domain superfamily	SUPERFAMILY	48537	Phospholipase C/P1 nuclease	coord: 25..289

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G14660.1	CSPI01G14660.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006308	DNA catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004521	endoribonuclease activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0000014	single-stranded DNA endodeoxyribonuclease activity
molecular_function	GO:0004519	endonuclease activity
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds