CSPI01G14110 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G14110
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Magnesium transporter
Location	Chr1: 9687060 .. 9689854 (-)
RNA-Seq Expression	CSPI01G14110
Synteny	CSPI01G14110

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGCTCCCATTTCTTCCAACAACCAATCCTTCTTTAATGTTCATAACCAAAGAGCTCAAACCGCTCTCCAATGGCCCTCTCTTTATCCCTACAACAACTCATTCGCTTCCCTCCTCAACCTCCATCTCCATCCTCTCACCTCGCCTCTCTCTCCCGCTTCTTCCCATCTCCATGCCTTTCCTCTTTCGCTCCGTCCCGAGACTATGCCGTCAAGTTATCTTCCAGAACAAAGTGTTTCTCCATGTCCACTGAGGAGGACAAGTGGAGTGAATCTGATGCGTTTGTCTCCGATGTCGACGAAGGGGGAGATCTTGAGGATGACATTGATATTAGTGTTCGGGAAGACAGCCCTGTGGCCACTGCTGCTTCTGCGCAGAGGATTTCTTCTTCACCCAGTGATTATCTCTCGCTTGCGATTCGTGAGCAGGTTTATGAGGTCTGTTTCTTTGATGCATTTTTCTATTTTTAGTTCATTTTGGATGTGTTTGCTGCTACTTTGAATTGCGTTCGATTGAAGTGGGGAATTTGACTCTCAAACCGAAAATTTCAATCAAGTTGAGATAGCCTGTTCAAATGCCTAACTAATTATTTGATTCTTGAGGGAAATTAGCTGAGCAGTGTTGTGTCTACCAATGCCTAATCGTCGCTAACTGGGGTCTTTTTTCTTTTTGTTGATGTTGAAGAACGTTTAAACGGGTTAATAGTAACTAACTGTAAGGTTCCTCGCATATGATGCTATGTTATAAAAGGTTTAGTATTAAAATTTTAAACGGTTGTAAGTGCTTCTAATCTTGTAGTAATTTAATGTTTCCTTCATTGATTTTTGAACATAAAAAACATTGACAGCATACAACTTTATTTTATTTTATTTTTTTATTTTAAAGGAGGACAGATTCAAACCTCTAAAGATATCTTTAGAGCGAACAAATGATGTAAAGTTATGACATTATATTGTTTTTTTTGGATGAAAATAACACCTTTAATTGGGAAAGAATAAAAGAATACAAGTACATACAAAAAAAAACCAACCCAACAAAACACTTCATTAAAAAATAACCAAGTAAAATGTTGCCTATGCAATAATTATAGAAGCTCCTTGAAATCAAAGCTTAAGGGGACATGTGAAATCTAAGCAAACAAATCTCACTAAGCCCTCTCTCCCTACCCTGAAAAACTCGATCGTTTCTCTCCTCCAAATGTCCCAAATAACAGCGCACACCCCGGCAAGCCATAAGAAACCCCTTTGTCTCTTTGGTGACCATACGTACATTTGATTAGTTTAGAGAATAAATCATATAAAATAATCACTACACCCATCAAAGAAATAATACAAAGTAATTACTTTTGAGGGGTTCTACTTTTGCACTCTTCTTTGAAGTCACTATTTTTTTTGGATATGAAAAAAGGTTCTGTTTGAAGGGCGGAACATTTAAATTTTTTTAGCTCAAGTTAAGACCTTGTGCTGTAATCCTTCCACCGGTTGGAGTTAATGGTTTGGAGGTAGGCTAGTTATAGTTTTGATTCCAAGATCAAATCTTTTCCTGTTAGAGATATATTTATGGGTTTCTTTCTTTGATAAAGGGAAATTCTTACAGATGTTCATCTTTATTGACCACCTAGGTGTTAGAGGTTAAAGCTGATGGAACCGTATCCACCAGGAAGGTTAACAGACGGCAGCTGTTGAAGTCAAGTGGTGGGTAGATATTTCCAGAAAGTTCAGCTTCACATTTCAATGCTTAGTTTCTGAATTTATGCTGTTGAAAACTTTTAGCCTGCATTGACTTATGAATACTTTCTTTTTTGGAATATCAATTACTACTGGATGATCACTGTTGTTTTTTCTGGATTCACACTGATAGATAACCTGGGATGACAAATAATATTACGACCTACTTTTTGTTAAGAAAATATATCTTGTCGTTTCATTATTTTCCTCTTGCTGATATATGTATTTTCTAATTGTCAGGGCTTCGTCCTCGTGATGTCCGAAGTGTTGATCCATCTTTATTTTTGACAAACTCAATGCCTACATTACTGGTACATTAATTTTCATCTTTTTTAATTTGTGGTCTTCTTTTTATGTTGAAGAATTAGCATATGGGATAAACATATGAAATTAAGAAATTGGAGTCTATAAATAATACTCAGCAACAATTATATTTGTGGTTCACATCCCAGGTACGTGAACATGCTATTTTGCTTAATCTGGGATCGTTGCGAGCAATAGCCATGCAGGATTGTGTCCTCATATTCGATCATAACCGGTATCCATCTGACTTGAAAATGGTGCCATGTTTATTTCTCATACAAAATTTCGATATTGCATTTCTTTTTGCATGATGGTGATCATTTTCAGTCCAGGAGGACAAGCTTTTATTGAATCATTGTTGCCTCGATTGAACCCAAAAAACATGAATGGAGTGCCAGCAATGCCATTTGAACTCGAGGTTAGTGATTCTTGTTAATATAATGCGTCTTTACATCATATAAATAGAGTTAGCAGATGAAAGCAAAATTATCTTACAAACTGTACTTCTGTAGCTCTCTACATCTAACAATAAGATGTTTCCTGGTTGGAATTTGGTCCCCGATAAGCTTACCCCTCAAAGCAAAGAAAAATTACCTCTAACTGCCTTTCAGCATTCTTATGTCATTCTGTGAATCCCAATGGAAATTGCTGAGAAGATCAGATGATTATTCTAAGAAGCAGGGACACGGATACGAGACACGAATACGACACAGAGACTGCGACATGCCATATTTTAACAATCTAGAATACGACACGTGAAGGGCGC

mRNA sequence

AAGCTCCCATTTCTTCCAACAACCAATCCTTCTTTAATGTTCATAACCAAAGAGCTCAAACCGCTCTCCAATGGCCCTCTCTTTATCCCTACAACAACTCATTCGCTTCCCTCCTCAACCTCCATCTCCATCCTCTCACCTCGCCTCTCTCTCCCGCTTCTTCCCATCTCCATGCCTTTCCTCTTTCGCTCCGTCCCGAGACTATGCCGTCAAGTTATCTTCCAGAACAAAGTGTTTCTCCATGTCCACTGAGGAGGACAAGTGGAGTGAATCTGATGCGTTTGTCTCCGATGTCGACGAAGGGGGAGATCTTGAGGATGACATTGATATTAGTGTTCGGGAAGACAGCCCTGTGGCCACTGCTGCTTCTGCGCAGAGGATTTCTTCTTCACCCAGTGATTATCTCTCGCTTGCGATTCGTGAGCAGGTTTATGAGGTGTTAGAGGTTAAAGCTGATGGAACCGTATCCACCAGGAAGGTTAACAGACGGCAGCTGTTGAAGTCAAGTGGGCTTCGTCCTCGTGATGTCCGAAGTGTTGATCCATCTTTATTTTTGACAAACTCAATGCCTACATTACTGGTACGTGAACATGCTATTTTGCTTAATCTGGGATCGTTGCGAGCAATAGCCATGCAGGATTGTGTCCTCATATTCGATCATAACCGTCCAGGAGGACAAGCTTTTATTGAATCATTGTTGCCTCGATTGAACCCAAAAAACATGAATGGAGTGCCAGCAATGCCATTTGAACTCGAGCTCTCTACATCTAACAATAAGATGTTTCCTGGTTGGAATTTGGTCCCCGATAAGCTTACCCCTCAAAGCAAAGAAAAATTACCTCTAACTGCCTTTCAGCATTCTTATGTCATTCTGTGAATCCCAATGGAAATTGCTGAGAAGATCAGATGATTATTCTAAGAAGCAGGGACACGGATACGAGACACGAATACGACACAGAGACTGCGACATGCCATATTTTAACAATCTAGAATACGACACGTGAAGGGCGC

Coding sequence (CDS)

ATGGCCCTCTCTTTATCCCTACAACAACTCATTCGCTTCCCTCCTCAACCTCCATCTCCATCCTCTCACCTCGCCTCTCTCTCCCGCTTCTTCCCATCTCCATGCCTTTCCTCTTTCGCTCCGTCCCGAGACTATGCCGTCAAGTTATCTTCCAGAACAAAGTGTTTCTCCATGTCCACTGAGGAGGACAAGTGGAGTGAATCTGATGCGTTTGTCTCCGATGTCGACGAAGGGGGAGATCTTGAGGATGACATTGATATTAGTGTTCGGGAAGACAGCCCTGTGGCCACTGCTGCTTCTGCGCAGAGGATTTCTTCTTCACCCAGTGATTATCTCTCGCTTGCGATTCGTGAGCAGGTTTATGAGGTGTTAGAGGTTAAAGCTGATGGAACCGTATCCACCAGGAAGGTTAACAGACGGCAGCTGTTGAAGTCAAGTGGGCTTCGTCCTCGTGATGTCCGAAGTGTTGATCCATCTTTATTTTTGACAAACTCAATGCCTACATTACTGGTACGTGAACATGCTATTTTGCTTAATCTGGGATCGTTGCGAGCAATAGCCATGCAGGATTGTGTCCTCATATTCGATCATAACCGTCCAGGAGGACAAGCTTTTATTGAATCATTGTTGCCTCGATTGAACCCAAAAAACATGAATGGAGTGCCAGCAATGCCATTTGAACTCGAGCTCTCTACATCTAACAATAAGATGTTTCCTGGTTGGAATTTGGTCCCCGATAAGCTTACCCCTCAAAGCAAAGAAAAATTACCTCTAACTGCCTTTCAGCATTCTTATGTCATTCTGTGA

Protein sequence

MALSLSLQQLIRFPPQPPSPSSHLASLSRFFPSPCLSSFAPSRDYAVKLSSRTKCFSMSTEEDKWSESDAFVSDVDEGGDLEDDIDISVREDSPVATAASAQRISSSPSDYLSLAIREQVYEVLEVKADGTVSTRKVNRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDHNRPGGQAFIESLLPRLNPKNMNGVPAMPFELELSTSNNKMFPGWNLVPDKLTPQSKEKLPLTAFQHSYVIL*

Homology

[Close]

BLAST of CSPI01G14110 vs. ExPASy Swiss-Prot
Match: Q058N4 (Magnesium transporter MRS2-11, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MRS2-11 PE=1 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 7.8e-53
Identity = 133/231 (57.58%), Postives = 165/231 (71.43%), Query Frame = 0


Query: 4   SLSLQQLIRFPPQPPSPSSHLASLSRFFPSPCLSSFAPSRDYAVKLSSRTKCFSMS--TE 63
           S SL  L+  P   PS S  L++LSR           P    A+K+ SR+KCF+ S  T 
Sbjct: 24  SPSLHYLL--PGSSPSFSLQLSALSR----------TPIYFEALKVLSRSKCFAKSPTTA 83

Query: 64  EDKWSESDAF-VSDVDEGGD--LEDDIDISVREDSPVATAASAQRISSSPSDYLSLAIRE 123
           ED   + ++  VSD D+G D    D  +   R+DS    ++S    SSS SD  SL IRE
Sbjct: 84  EDFVGDYESLNVSDDDDGSDSNSSDGDNGGGRDDSKKIDSSS----SSSSSDSTSLGIRE 143

Query: 124 QVYEVLEVKADGTVSTRKVNRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVREHAILL 183
            VYEV+EVKA G +STRK+NRRQLLKSSGLRPRD+RSVDPSLF+TNS+P+LLVREHAILL
Sbjct: 144 PVYEVVEVKATGAISTRKINRRQLLKSSGLRPRDIRSVDPSLFMTNSVPSLLVREHAILL 203

Query: 184 NLGSLRAIAMQDCVLIFDHNRPGGQAFIESLLPRLNPKNMNGVPAMPFELE 230
           NLGSLRAIAM+D VLIFD+NR GG+AF+++L+PRLNP++MNG P+MPFELE
Sbjct: 204 NLGSLRAIAMRDRVLIFDYNRRGGRAFVDTLMPRLNPRSMNGGPSMPFELE 238

[Close]

BLAST of CSPI01G14110 vs. ExPASy Swiss-Prot
Match: B8APK3 (Magnesium transporter MRS2-A, chloroplastic OS=Oryza sativa subsp. indica OX=39946 GN=MRS2-A PE=3 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 6.0e-45
Identity = 103/172 (59.88%), Postives = 133/172 (77.33%), Query Frame = 0


Query: 64  KWSESDAFVSDVDEGGDLEDDIDISVREDSP--VATAASAQRISSS---PSDYLSLAIRE 123
           K  E +A  + V+E G++E    +   ED P    +  +A R S S    +DY+SL IRE
Sbjct: 87  KDEEEEAAAAAVEEEGEVE----VRREEDKPGDDGSREAAARGSGSGRFSADYISLGIRE 146

Query: 124 QVYEVLEVKADGTVSTRKVNRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVREHAILL 183
            VYEV+EVK++G +ST+K++RRQLLKSSGLR RD RSVDPSL+L NSMP+LLVRE AIL+
Sbjct: 147 PVYEVIEVKSNGRMSTKKISRRQLLKSSGLRLRDTRSVDPSLWLMNSMPSLLVREQAILV 206

Query: 184 NLGSLRAIAMQDCVLIFDHNRPGGQAFIESLLPRLNPKNMNGVPAMPFELEL 231
           NLGSLRAIAM + VLIF++N PGG+AF++SLLPRLNP+N+NG PAMPF+LE+
Sbjct: 207 NLGSLRAIAMHERVLIFNYNSPGGKAFLDSLLPRLNPRNINGGPAMPFQLEV 254

[Close]

BLAST of CSPI01G14110 vs. ExPASy Swiss-Prot
Match: Q9AUK4 (Magnesium transporter MRS2-A, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=MRS2-A PE=2 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 6.0e-45
Identity = 103/172 (59.88%), Postives = 133/172 (77.33%), Query Frame = 0


Query: 64  KWSESDAFVSDVDEGGDLEDDIDISVREDSP--VATAASAQRISSS---PSDYLSLAIRE 123
           K  E +A  + V+E G++E    +   ED P    +  +A R S S    +DY+SL IRE
Sbjct: 87  KDEEEEAAAAAVEEEGEVE----VRREEDKPGDDGSREAAARGSGSGRFSADYISLGIRE 146

Query: 124 QVYEVLEVKADGTVSTRKVNRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVREHAILL 183
            VYEV+EVK++G +ST+K++RRQLLKSSGLR RD RSVDPSL+L NSMP+LLVRE AIL+
Sbjct: 147 PVYEVIEVKSNGRMSTKKISRRQLLKSSGLRLRDTRSVDPSLWLMNSMPSLLVREQAILV 206

Query: 184 NLGSLRAIAMQDCVLIFDHNRPGGQAFIESLLPRLNPKNMNGVPAMPFELEL 231
           NLGSLRAIAM + VLIF++N PGG+AF++SLLPRLNP+N+NG PAMPF+LE+
Sbjct: 207 NLGSLRAIAMHERVLIFNYNSPGGKAFLDSLLPRLNPRNINGGPAMPFQLEV 254

[Close]

BLAST of CSPI01G14110 vs. ExPASy Swiss-Prot
Match: Q7SFQ9 (Mitochondrial inner membrane magnesium transporter mrs2 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=mrs2 PE=3 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 1.9e-06
Identity = 45/152 (29.61%), Postives = 77/152 (50.66%), Query Frame = 0


Query: 82  EDDIDISVREDSPVATAASAQRISSSPSDYLSLAIREQVYEVLEVKADGTV--STRKVNR 141
           +DDI +++ E+S     +  QR + +    +   +R       EV  +G V     ++ +
Sbjct: 150 DDDIRVALEEES----GSIFQRRALTAKAAMDPRLR-----CTEVDENGNVVMVDGELKK 209

Query: 142 RQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFD--- 201
            +L+   GL PRD+R +D S     ++P +L+R  AILLNL  L+ +   DCVL+FD   
Sbjct: 210 SELIAKYGLLPRDLRKIDSS-----NLPHILIRPSAILLNLLHLKVLIKHDCVLLFDVYG 269

Query: 202 -HNRPGGQAFIESLLPRLNPKNMNGVPAMPFE 228
             +     AF+  L  +L  K  +G  ++P+E
Sbjct: 270 SKSSYPQSAFMYDLQGKLQQKQSSGANSLPYE 287

[Close]

BLAST of CSPI01G14110 vs. ExPASy Swiss-Prot
Match: Q6C8H7 (Mitochondrial inner membrane magnesium transporter LPE10 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=LPE10 PE=3 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 4.2e-06
Identity = 29/74 (39.19%), Postives = 48/74 (64.86%), Query Frame = 0


Query: 123 VLEVKADGTVSTRKVNRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVREHAILLNLGS 182
           V +   + TV++ +  R +LL   GL PRD+R +D    + + +PT+LVR+++IL+NL  
Sbjct: 112 VFDSDGNVTVASGEFKRTELLNKHGLLPRDLRKLDTG--VNSIVPTILVRDNSILINLLH 171

Query: 183 LRAIAMQDCVLIFD 197
           +RA+   D VL+FD
Sbjct: 172 IRALIKADKVLLFD 183

[Close]

BLAST of CSPI01G14110 vs. ExPASy TrEMBL
Match: A0A5A7U838 (Magnesium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1371G00610 PE=3 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 6.6e-103
Identity = 213/240 (88.75%), Postives = 222/240 (92.50%), Query Frame = 0


Query: 1   MALSLSLQQLIRFPPQPPSPSSH------LASLSRFFPSPCLSSFAPSRDYAVKLSSRTK 60
           MALSLSL QLIRFPP+P S SSH      L S+SRFF SPCLSS APSRD A+KLSSRTK
Sbjct: 1   MALSLSL-QLIRFPPRPLSSSSHHLLISDLTSVSRFFRSPCLSSLAPSRDCALKLSSRTK 60

Query: 61  CFSMSTEEDKWSESDAFVSDVDEGGDLEDDIDISVREDSPVATAASAQRI----SSSPSD 120
           CFSMSTEE++WSES+AFVSDVDEGGDLEDDIDISVREDS VATAASAQRI    SSSPSD
Sbjct: 61  CFSMSTEEEQWSESEAFVSDVDEGGDLEDDIDISVREDSSVATAASAQRISSSSSSSPSD 120

Query: 121 YLSLAIREQVYEVLEVKADGTVSTRKVNRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLL 180
           YLSLAIREQVYEVLEVKA+GTVSTRKVNRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLL
Sbjct: 121 YLSLAIREQVYEVLEVKANGTVSTRKVNRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLL 180

Query: 181 VREHAILLNLGSLRAIAMQDCVLIFDHNRPGGQAFIESLLPRLNPKNMNGVPAMPFELEL 231
           VREHAILLNLGSLRAIAMQDCVLIFD+NRPGGQAFIESLLPRLNPKNMNGVPAMPFELE+
Sbjct: 181 VREHAILLNLGSLRAIAMQDCVLIFDYNRPGGQAFIESLLPRLNPKNMNGVPAMPFELEV 239

[Close]

BLAST of CSPI01G14110 vs. ExPASy TrEMBL
Match: A0A1S3CI47 (Magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103500679 PE=3 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 4.3e-102
Identity = 212/240 (88.33%), Postives = 221/240 (92.08%), Query Frame = 0


Query: 1   MALSLSLQQLIRFPPQPPSPSSH------LASLSRFFPSPCLSSFAPSRDYAVKLSSRTK 60
           MALSLSL QLIRFPP+P S SSH      L S+SRFF SPCLSS APSRD A+KLSSRTK
Sbjct: 1   MALSLSL-QLIRFPPRPLSSSSHHLLISDLTSVSRFFRSPCLSSLAPSRDCALKLSSRTK 60

Query: 61  CFSMSTEEDKWSESDAFVSDVDEGGDLEDDIDISVREDSPVATAASAQRI----SSSPSD 120
           CFSMSTEE++WSES+AFVSDVDEGGDLEDDIDISVREDS VATAASAQRI    SSSPSD
Sbjct: 61  CFSMSTEEEQWSESEAFVSDVDEGGDLEDDIDISVREDSSVATAASAQRISSSSSSSPSD 120

Query: 121 YLSLAIREQVYEVLEVKADGTVSTRKVNRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLL 180
           YLSLAIREQVYEVLEVKA+GTVSTRKVNRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLL
Sbjct: 121 YLSLAIREQVYEVLEVKANGTVSTRKVNRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLL 180

Query: 181 VREHAILLNLGSLRAIAMQDCVLIFDHNRPGGQAFIESLLPRLNPKNMNGVPAMPFELEL 231
           VREHAILLNLGSLRAIAMQDCVLIFD+NRPGGQAFIESLLPRLNPK MNGVPAMPFELE+
Sbjct: 181 VREHAILLNLGSLRAIAMQDCVLIFDYNRPGGQAFIESLLPRLNPKXMNGVPAMPFELEV 239

[Close]

BLAST of CSPI01G14110 vs. ExPASy TrEMBL
Match: A0A6J1HIJ3 (magnesium transporter MRS2-11, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111464721 PE=3 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 2.5e-86
Identity = 189/237 (79.75%), Postives = 204/237 (86.08%), Query Frame = 0


Query: 1   MALSLSLQQLIRFPPQPPSPSSH------LASLSRFFPSPCLSSFAPSRDYAVKLSSRTK 60
           MAL  SL QLIRFPPQ  S SS       L S+SRFF SP LSS  PSR   + LSSRTK
Sbjct: 1   MALYPSL-QLIRFPPQALSSSSRHLLISDLGSVSRFFRSPWLSSLPPSRKCRMILSSRTK 60

Query: 61  CFSMSTEEDKWSESDAFVSDVDEGGDLEDDIDISVREDSPVATAASAQRI-SSSPSDYLS 120
           CFSMSTEED+WSES+A +SDVDEGGD ED  DI V+++S V +AASAQRI SSSPSDYLS
Sbjct: 61  CFSMSTEEDQWSESEALMSDVDEGGDRED--DIGVQQESSVVSAASAQRISSSSPSDYLS 120

Query: 121 LAIREQVYEVLEVKADGTVSTRKVNRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVRE 180
           LAIRE VYEVLEVKA+G VSTRK+NRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVRE
Sbjct: 121 LAIRESVYEVLEVKANGAVSTRKINRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVRE 180

Query: 181 HAILLNLGSLRAIAMQDCVLIFDHNRPGGQAFIESLLPRLNPKNMNGVPAMPFELEL 231
           +AILLNLGSLRAIAM DCVLIFD+NRPGGQAFIESLLPRLNPK+MNGVPAMPFELE+
Sbjct: 181 NAILLNLGSLRAIAMPDCVLIFDYNRPGGQAFIESLLPRLNPKSMNGVPAMPFELEV 234

[Close]

BLAST of CSPI01G14110 vs. ExPASy TrEMBL
Match: A0A6J1HK11 (Magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111464814 PE=3 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 2.5e-86
Identity = 189/237 (79.75%), Postives = 204/237 (86.08%), Query Frame = 0


Query: 1   MALSLSLQQLIRFPPQPPSPSSH------LASLSRFFPSPCLSSFAPSRDYAVKLSSRTK 60
           MAL  SL QLIRFPPQ  S SS       L S+SRFF SP LSS  PSR   + LSSRTK
Sbjct: 1   MALYPSL-QLIRFPPQALSSSSRHLLISDLGSVSRFFRSPWLSSLPPSRKCRMILSSRTK 60

Query: 61  CFSMSTEEDKWSESDAFVSDVDEGGDLEDDIDISVREDSPVATAASAQRI-SSSPSDYLS 120
           CFSMSTEED+WSES+A +SDVDEGGD ED  DI V+++S V +AASAQRI SSSPSDYLS
Sbjct: 61  CFSMSTEEDQWSESEALMSDVDEGGDRED--DIGVQQESSVVSAASAQRISSSSPSDYLS 120

Query: 121 LAIREQVYEVLEVKADGTVSTRKVNRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVRE 180
           LAIRE VYEVLEVKA+G VSTRK+NRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVRE
Sbjct: 121 LAIRESVYEVLEVKANGAVSTRKINRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVRE 180

Query: 181 HAILLNLGSLRAIAMQDCVLIFDHNRPGGQAFIESLLPRLNPKNMNGVPAMPFELEL 231
           +AILLNLGSLRAIAM DCVLIFD+NRPGGQAFIESLLPRLNPK+MNGVPAMPFELE+
Sbjct: 181 NAILLNLGSLRAIAMPDCVLIFDYNRPGGQAFIESLLPRLNPKSMNGVPAMPFELEV 234

[Close]

BLAST of CSPI01G14110 vs. ExPASy TrEMBL
Match: A0A6J1I3J1 (magnesium transporter MRS2-11, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111469313 PE=3 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 1.1e-84
Identity = 187/237 (78.90%), Postives = 203/237 (85.65%), Query Frame = 0


Query: 1   MALSLSLQQLIRFPPQP-PSPSSH-----LASLSRFFPSPCLSSFAPSRDYAVKLSSRTK 60
           MAL  SL QLIRFPPQ  PS S H     L S+SRFF SP LSS  PS+   + LSSRTK
Sbjct: 1   MALYPSL-QLIRFPPQALPSSSRHLLISDLGSVSRFFRSPWLSSLPPSQKCRMILSSRTK 60

Query: 61  CFSMSTEEDKWSESDAFVSDVDEGGDLEDDIDISVREDSPVATAASAQRI-SSSPSDYLS 120
           CFSMSTEED+WSES+A +SDVDE GD ED  DI V+++S V +AAS QRI SSSPSDYLS
Sbjct: 61  CFSMSTEEDQWSESEALMSDVDERGDRED--DIGVQQESSVVSAASGQRISSSSPSDYLS 120

Query: 121 LAIREQVYEVLEVKADGTVSTRKVNRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVRE 180
           LAIRE VYEVLEVKA+G VSTRK+NRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVRE
Sbjct: 121 LAIRESVYEVLEVKANGAVSTRKINRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVRE 180

Query: 181 HAILLNLGSLRAIAMQDCVLIFDHNRPGGQAFIESLLPRLNPKNMNGVPAMPFELEL 231
           +AILLNLGSLRAIAM DCVLIFD+NRPGGQAFIESLLPRLNPK+MNGVPAMPFELE+
Sbjct: 181 NAILLNLGSLRAIAMPDCVLIFDYNRPGGQAFIESLLPRLNPKSMNGVPAMPFELEV 234

[Close]

BLAST of CSPI01G14110 vs. NCBI nr
Match: XP_031741504.1 (magnesium transporter MRS2-11, chloroplastic [Cucumis sativus] >XP_031741505.1 magnesium transporter MRS2-11, chloroplastic [Cucumis sativus] >XP_031741515.1 magnesium transporter MRS2-11, chloroplastic [Cucumis sativus] >XP_031741526.1 magnesium transporter MRS2-11, chloroplastic [Cucumis sativus] >XP_031741533.1 magnesium transporter MRS2-11, chloroplastic [Cucumis sativus] >XP_031741541.1 magnesium transporter MRS2-11, chloroplastic [Cucumis sativus] >XP_031741543.1 magnesium transporter MRS2-11, chloroplastic [Cucumis sativus] >XP_031741545.1 magnesium transporter MRS2-11, chloroplastic [Cucumis sativus] >XP_031741547.1 magnesium transporter MRS2-11, chloroplastic [Cucumis sativus] >KAE8652790.1 hypothetical protein Csa_022786 [Cucumis sativus])

HSP 1 Score: 427.6 bits (1098), Expect = 8.2e-116
Identity = 227/230 (98.70%), Postives = 229/230 (99.57%), Query Frame = 0


Query: 1   MALSLSLQQLIRFPPQPPSPSSHLASLSRFFPSPCLSSFAPSRDYAVKLSSRTKCFSMST 60
           MALSLSL QLIRFPPQPPSPSSHLASLSRFFPSPCLSSFAPSRDYAVKLSSRTKCFSMST
Sbjct: 1   MALSLSL-QLIRFPPQPPSPSSHLASLSRFFPSPCLSSFAPSRDYAVKLSSRTKCFSMST 60

Query: 61  EEDKWSESDAFVSDVDEGGDLEDDIDISVREDSPVATAASAQRISSSPSDYLSLAIREQV 120
           EEDKWSESDAFVSDVDEGGDLEDDIDISVREDSPVATAASAQRISSSPSDYLSLAIREQV
Sbjct: 61  EEDKWSESDAFVSDVDEGGDLEDDIDISVREDSPVATAASAQRISSSPSDYLSLAIREQV 120

Query: 121 YEVLEVKADGTVSTRKVNRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVREHAILLNL 180
           YEVLEVKA+GTVSTRKVNRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVREHAILLNL
Sbjct: 121 YEVLEVKANGTVSTRKVNRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVREHAILLNL 180

Query: 181 GSLRAIAMQDCVLIFDHNRPGGQAFIESLLPRLNPKNMNGVPAMPFELEL 231
           GSLRAIAMQDCVLIFDHNRPGGQAFIESLLPRLNPKNMNGVPAMPFELE+
Sbjct: 181 GSLRAIAMQDCVLIFDHNRPGGQAFIESLLPRLNPKNMNGVPAMPFELEV 229

[Close]

BLAST of CSPI01G14110 vs. NCBI nr
Match: XP_031740723.1 (magnesium transporter MRS2-11, chloroplastic isoform X3 [Cucumis sativus] >KAE8649462.1 hypothetical protein Csa_018882 [Cucumis sativus])

HSP 1 Score: 400.6 bits (1028), Expect = 1.1e-107
Identity = 211/214 (98.60%), Postives = 211/214 (98.60%), Query Frame = 0


Query: 18  PSPSSHLASLSRFFPSPCLSSFAPSRDYAVKLSSRTKCFSMSTEEDKWSESDAFVSDVDE 77
           PSPSSHLASLSRFFPSPCLSSFAPS DYAVKLSSRTKCFSMSTEEDKWSESDAFVSDVDE
Sbjct: 25  PSPSSHLASLSRFFPSPCLSSFAPSPDYAVKLSSRTKCFSMSTEEDKWSESDAFVSDVDE 84

Query: 78  GGDLEDDIDISVREDSPVATAASAQRISSSPSDYLSLAIREQVYEVLEVKADGTVSTRKV 137
           GGDLEDDIDISVREDSPVATAASAQRISSSPSDYLSLAI EQVYEVLEVKADGTVSTRKV
Sbjct: 85  GGDLEDDIDISVREDSPVATAASAQRISSSPSDYLSLAIPEQVYEVLEVKADGTVSTRKV 144

Query: 138 NRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDH 197
           NRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDH
Sbjct: 145 NRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDH 204

Query: 198 NRPGGQAFIESLLPRLNPKNMNGVPAMPFELELS 232
           NRPGGQAFIESLLPRLNPKNMNGVPAMPFELE S
Sbjct: 205 NRPGGQAFIESLLPRLNPKNMNGVPAMPFELESS 238

[Close]

BLAST of CSPI01G14110 vs. NCBI nr
Match: XP_031740722.1 (magnesium transporter MRS2-11, chloroplastic isoform X2 [Cucumis sativus])

HSP 1 Score: 399.8 bits (1026), Expect = 1.8e-107
Identity = 210/212 (99.06%), Postives = 210/212 (99.06%), Query Frame = 0


Query: 18  PSPSSHLASLSRFFPSPCLSSFAPSRDYAVKLSSRTKCFSMSTEEDKWSESDAFVSDVDE 77
           PSPSSHLASLSRFFPSPCLSSFAPS DYAVKLSSRTKCFSMSTEEDKWSESDAFVSDVDE
Sbjct: 25  PSPSSHLASLSRFFPSPCLSSFAPSPDYAVKLSSRTKCFSMSTEEDKWSESDAFVSDVDE 84

Query: 78  GGDLEDDIDISVREDSPVATAASAQRISSSPSDYLSLAIREQVYEVLEVKADGTVSTRKV 137
           GGDLEDDIDISVREDSPVATAASAQRISSSPSDYLSLAI EQVYEVLEVKADGTVSTRKV
Sbjct: 85  GGDLEDDIDISVREDSPVATAASAQRISSSPSDYLSLAIPEQVYEVLEVKADGTVSTRKV 144

Query: 138 NRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDH 197
           NRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDH
Sbjct: 145 NRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDH 204

Query: 198 NRPGGQAFIESLLPRLNPKNMNGVPAMPFELE 230
           NRPGGQAFIESLLPRLNPKNMNGVPAMPFELE
Sbjct: 205 NRPGGQAFIESLLPRLNPKNMNGVPAMPFELE 236

[Close]

BLAST of CSPI01G14110 vs. NCBI nr
Match: XP_031740721.1 (magnesium transporter MRS2-11, chloroplastic isoform X1 [Cucumis sativus])

HSP 1 Score: 399.8 bits (1026), Expect = 1.8e-107
Identity = 210/212 (99.06%), Postives = 210/212 (99.06%), Query Frame = 0


Query: 18  PSPSSHLASLSRFFPSPCLSSFAPSRDYAVKLSSRTKCFSMSTEEDKWSESDAFVSDVDE 77
           PSPSSHLASLSRFFPSPCLSSFAPS DYAVKLSSRTKCFSMSTEEDKWSESDAFVSDVDE
Sbjct: 25  PSPSSHLASLSRFFPSPCLSSFAPSPDYAVKLSSRTKCFSMSTEEDKWSESDAFVSDVDE 84

Query: 78  GGDLEDDIDISVREDSPVATAASAQRISSSPSDYLSLAIREQVYEVLEVKADGTVSTRKV 137
           GGDLEDDIDISVREDSPVATAASAQRISSSPSDYLSLAI EQVYEVLEVKADGTVSTRKV
Sbjct: 85  GGDLEDDIDISVREDSPVATAASAQRISSSPSDYLSLAIPEQVYEVLEVKADGTVSTRKV 144

Query: 138 NRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDH 197
           NRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDH
Sbjct: 145 NRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDH 204

Query: 198 NRPGGQAFIESLLPRLNPKNMNGVPAMPFELE 230
           NRPGGQAFIESLLPRLNPKNMNGVPAMPFELE
Sbjct: 205 NRPGGQAFIESLLPRLNPKNMNGVPAMPFELE 236

[Close]

BLAST of CSPI01G14110 vs. NCBI nr
Match: KAA0052093.1 (magnesium transporter MRS2-11 [Cucumis melo var. makuwa] >TYK00707.1 magnesium transporter MRS2-11 [Cucumis melo var. makuwa])

HSP 1 Score: 383.6 bits (984), Expect = 1.4e-102
Identity = 213/240 (88.75%), Postives = 222/240 (92.50%), Query Frame = 0


Query: 1   MALSLSLQQLIRFPPQPPSPSSH------LASLSRFFPSPCLSSFAPSRDYAVKLSSRTK 60
           MALSLSL QLIRFPP+P S SSH      L S+SRFF SPCLSS APSRD A+KLSSRTK
Sbjct: 1   MALSLSL-QLIRFPPRPLSSSSHHLLISDLTSVSRFFRSPCLSSLAPSRDCALKLSSRTK 60

Query: 61  CFSMSTEEDKWSESDAFVSDVDEGGDLEDDIDISVREDSPVATAASAQRI----SSSPSD 120
           CFSMSTEE++WSES+AFVSDVDEGGDLEDDIDISVREDS VATAASAQRI    SSSPSD
Sbjct: 61  CFSMSTEEEQWSESEAFVSDVDEGGDLEDDIDISVREDSSVATAASAQRISSSSSSSPSD 120

Query: 121 YLSLAIREQVYEVLEVKADGTVSTRKVNRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLL 180
           YLSLAIREQVYEVLEVKA+GTVSTRKVNRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLL
Sbjct: 121 YLSLAIREQVYEVLEVKANGTVSTRKVNRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLL 180

Query: 181 VREHAILLNLGSLRAIAMQDCVLIFDHNRPGGQAFIESLLPRLNPKNMNGVPAMPFELEL 231
           VREHAILLNLGSLRAIAMQDCVLIFD+NRPGGQAFIESLLPRLNPKNMNGVPAMPFELE+
Sbjct: 181 VREHAILLNLGSLRAIAMQDCVLIFDYNRPGGQAFIESLLPRLNPKNMNGVPAMPFELEV 239

[Close]

BLAST of CSPI01G14110 vs. TAIR 10
Match: AT5G22830.1 (magnesium (Mg) transporter 10 )

HSP 1 Score: 208.8 bits (530), Expect = 5.6e-54
Identity = 133/231 (57.58%), Postives = 165/231 (71.43%), Query Frame = 0


Query: 4   SLSLQQLIRFPPQPPSPSSHLASLSRFFPSPCLSSFAPSRDYAVKLSSRTKCFSMS--TE 63
           S SL  L+  P   PS S  L++LSR           P    A+K+ SR+KCF+ S  T 
Sbjct: 24  SPSLHYLL--PGSSPSFSLQLSALSR----------TPIYFEALKVLSRSKCFAKSPTTA 83

Query: 64  EDKWSESDAF-VSDVDEGGD--LEDDIDISVREDSPVATAASAQRISSSPSDYLSLAIRE 123
           ED   + ++  VSD D+G D    D  +   R+DS    ++S    SSS SD  SL IRE
Sbjct: 84  EDFVGDYESLNVSDDDDGSDSNSSDGDNGGGRDDSKKIDSSS----SSSSSDSTSLGIRE 143

Query: 124 QVYEVLEVKADGTVSTRKVNRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVREHAILL 183
            VYEV+EVKA G +STRK+NRRQLLKSSGLRPRD+RSVDPSLF+TNS+P+LLVREHAILL
Sbjct: 144 PVYEVVEVKATGAISTRKINRRQLLKSSGLRPRDIRSVDPSLFMTNSVPSLLVREHAILL 203

Query: 184 NLGSLRAIAMQDCVLIFDHNRPGGQAFIESLLPRLNPKNMNGVPAMPFELE 230
           NLGSLRAIAM+D VLIFD+NR GG+AF+++L+PRLNP++MNG P+MPFELE
Sbjct: 204 NLGSLRAIAMRDRVLIFDYNRRGGRAFVDTLMPRLNPRSMNGGPSMPFELE 238

[Close]

BLAST of CSPI01G14110 vs. TAIR 10
Match: AT3G19640.1 (magnesium transporter 4 )

HSP 1 Score: 45.4 bits (106), Expect = 8.2e-05
Identity = 27/90 (30.00%), Postives = 48/90 (53.33%), Query Frame = 0


Query: 124 LEVKADGTVSTRKVNRRQLLKSSGLRPRDVRSVDPSLFLTNSMPTLLVREHAILLNLGSL 183
           L + + G    ++  +  +++ +GL  RD+R +DP   L +   T+L RE AI++NL  +
Sbjct: 48  LVLNSSGQSEPKEEGKHSIMRRTGLPARDLRILDP---LLSYPSTVLGRERAIVINLEHI 107

Query: 184 RAIAMQDCVLIFDHNRPGGQAFIESLLPRL 214
           +AI     VL+ +   P    FI+ L  R+
Sbjct: 108 KAIITAQEVLLLNSKDPSVSPFIDELQRRI 134

The following BLAST results are available for this feature:

BLAST of CSPI01G14110 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q058N4	7.8e-53	57.58	Magnesium transporter MRS2-11, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]
B8APK3	6.0e-45	59.88	Magnesium transporter MRS2-A, chloroplastic OS=Oryza sativa subsp. indica OX=399...	[more]
Q9AUK4	6.0e-45	59.88	Magnesium transporter MRS2-A, chloroplastic OS=Oryza sativa subsp. japonica OX=3...	[more]
Q7SFQ9	1.9e-06	29.61	Mitochondrial inner membrane magnesium transporter mrs2 OS=Neurospora crassa (st...	[more]
Q6C8H7	4.2e-06	39.19	Mitochondrial inner membrane magnesium transporter LPE10 OS=Yarrowia lipolytica ...	[more]

BLAST of CSPI01G14110 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U838	6.6e-103	88.75	Magnesium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13...	[more]
A0A1S3CI47	4.3e-102	88.33	Magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103500679 PE=3 SV=1	[more]
A0A6J1HIJ3	2.5e-86	79.75	magnesium transporter MRS2-11, chloroplastic-like OS=Cucurbita moschata OX=3662 ...	[more]
A0A6J1HK11	2.5e-86	79.75	Magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111464814 PE=3 SV=1	[more]
A0A6J1I3J1	1.1e-84	78.90	magnesium transporter MRS2-11, chloroplastic-like isoform X2 OS=Cucurbita maxima...	[more]

BLAST of CSPI01G14110 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031741504.1	8.2e-116	98.70	magnesium transporter MRS2-11, chloroplastic [Cucumis sativus] >XP_031741505.1 m...	[more]
XP_031740723.1	1.1e-107	98.60	magnesium transporter MRS2-11, chloroplastic isoform X3 [Cucumis sativus] >KAE86...	[more]
XP_031740722.1	1.8e-107	99.06	magnesium transporter MRS2-11, chloroplastic isoform X2 [Cucumis sativus]	[more]
XP_031740721.1	1.8e-107	99.06	magnesium transporter MRS2-11, chloroplastic isoform X1 [Cucumis sativus]	[more]
KAA0052093.1	1.4e-102	88.75	magnesium transporter MRS2-11 [Cucumis melo var. makuwa] >TYK00707.1 magnesium t...	[more]

BLAST of CSPI01G14110 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G22830.1	5.6e-54	57.58	magnesium (Mg) transporter 10	[more]
AT3G19640.1	8.2e-05	30.00	magnesium transporter 4	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	2.40.128.330		coord: 112..197 e-value: 1.6E-19 score: 71.2
None	No IPR available	PANTHER	PTHR13890:SF45	MG2+ TRANSPORTER PROTEIN, CORA-LIKE/ZINC TRANSPORT PROTEIN ZNTB-RELATED	coord: 37..230
IPR039204	Magnesium transporter MRS2-like	PANTHER	PTHR13890	RNA SPLICING PROTEIN MRS2, MITOCHONDRIAL	coord: 37..230

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G14110.1	CSPI01G14110.1	mRNA
CSPI01G14110.2	CSPI01G14110.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010960	magnesium ion homeostasis
biological_process	GO:1903830	magnesium ion transmembrane transport
biological_process	GO:0010117	photoprotection
biological_process	GO:0010027	thylakoid membrane organization
cellular_component	GO:0009941	chloroplast envelope
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0015095	magnesium ion transmembrane transporter activity