CSPI01G12010 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G12010
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionSphingolipid delta(4)-desaturase DES1-like
LocationChr1: 7574909 .. 7577757 (+)
RNA-Seq ExpressionCSPI01G12010
SyntenyCSPI01G12010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAGAATATAGAATATAGATCCCATTCCCACCAGCGACAAGTTGGCAATGGCACCGATATGAAGACGGCGATGTATACAACAGCGTCCAGTCGAACCCAACGTCCTTTTCTATATTTAGGAGTCATCTTCCATTCCATTTTCCTTTCCATATTTGGTCTTCTTCAATCCTCCGCCCATTCTTAAATCTTCAACAACCTTCCTCTCCTCTATAACTATCCCTTCCTTTCTCTTCCTCAATCCACATCCAATTTCATCAGCACCAGCCCCCAGGAGACGCAATTTCTTCCGGAGCATCTTCTGGGAGCAACCCCCACCCGGGGAATCACTCACAAACACAATGCCAGGTGGTGAAGAGAGAGATGATGGTGTTATGGCCACTGACTTCTTCTGGTCTTACACCGATGAACCTCACGCTTCCAGGAGACGACAAATTCTCTCTCAGTACCCTCAGATCCGGGAGCTCTTCGGTCCTGACCCCTGGGCTTTCTTCAAGGTTTTTTCTTTTCTTTTTTTTTTTTTAATTTCTTCTCTTTCTTGATTCTGAGAATGTAGAGAGTTGTGGTTATTTTCTTGTCAGGATGTTTTCATTTCGTGTTTAGATCTGTATTTCTGGTCTCTACACATGTCAATGCCTGAATTTGATCTGTGTATATTTGTTTTTATGTTTGATTTTGAACCTCATTTTTGCTACATTGTTGCGTTTTACTTTGTTTTTGTTTTTCTTTTATCATTCTGATTACTCGTGTTTGTTGCATCCATCGAAAATCGACATTTGAGATTCTGAACGCTTGTTATTGATGGATCTGTCCACTTCGCTGTTCTTTTTTGCTCTGGTTCAGTTATTTTTTTGCTACATTCCCATATCGTTTATTTATTTGTGTGTTTCTTCCTTTCTTCCTTCTTTGTATTACCAACGCCTGTCTAAAGTTTCCTGCTTTTTCAACTATTAGGCGAGCTTTGATTCAGGATTGTTTTGGTTTAAACTTTATTTAAAGTTATCCTTCTTTTCTTATTTGGAATTTAAAAGAATGCCCTGGAGGAAATTAACCCTCAACCTTTTTCACTAGGAAATGGTAAAGTTTAGTAGTACTCGATGAATTTTATTGTTGTTTGCTCTCTAGAGTTTTGGTAAATGTTTAGAGAGGAAATTAGCCCTCGACCCTTATTTCCTTGGTTGCCTGGCTCTAATATCTGATTTTAGCAGATGTTTAATCGTTTGATTAGGGTAGTCCATTGCAGTAGTTTATGGCTTGTATAGTTTCAAATGAGACCATTTATGATAATCAGAAGAATAAATTGTTGAAGTTAAATATTTAGACTAATGCTTCCTGCTGTTGTGAAGGGAAGAACTTTTTTTTTTTAATCGTTTGTTGTATTTGTTAAGAAGAGCAATTGATTGTAATTATTTAACCAACTGCATTGGCATTTTGATTCATCTGATGAAAAAAAATGAAGTCATGATCACGAAGAATGAGAACTTGGTGTGAACAGCCATTTAACATTCACGAACCTGACCTTGTAGTCATAAAGTTTAGCTATTGTAGTTTGGAGAACCAATCATTAATTTATTCTTACAGTATAAAAAGTTGGTCACTTTTTTCTTCTCGTTCTCATTCTCGCAATCTTGCAGATTTCTGTGGTTGTTCTGCTTCAGTTATGGACTGCTACGACCCTTCACAGTGCAGACTGGTTAAAGATATTGGCAATAGCCTACTTCTTTGGCTCTTTTCTCAACCACAACCTATTTTTAGCCATTCACGAGCTCAGTCACAACCTTGCCTTCTCAACACCTGATTACAACAAGTGGCTCGGGATTTTTGCTAATCTCCCCATTGGAGTGCCCATGTCCATCACATTTCAAAAGTATCACCTTGAGCACCATCGCTTTCAAGGAGTGGATGGCATGGATATGGACGTTCCAAGCCAGACCGAAGCTCATCTGGTGACTAATGTTGTCTCAAAAGCTATATGGGTTATCTTCCAACTATTCTTCTATGCACTCCGCCCACTGTTTCTCAAACCAAAGCCCCCAGGTCACTGGGAGTTCATCAACTTCATTATTCAGATAGCCCTTGATGTTACCATGGTTTACTTCTGGAGTTGGAAGTCTTTTGCTTACTTGATCCTCTCCACATTCGTTGGCGGTGGGATGCATCCAATGGCTGGTCACTTCATTTCCGAACATTATGTCTTCAATCCCAAACAGGAAACATATTCTTACTACGGTCCACTTAATCTTCTTACATGGAGTGTAGGTTACCACAATGAACACCATGACTTCCCCAGAATTCCAGGAAGCAAGCTTTATAAAGTGAAGGAGATTGCACCTGAATACTACAATGAATTAGATTCATACAAATCGTGGAGCCAGGTGATATACATGTACATTATGGACAAGACAGTGGGGCCATTCAGTAGGATGAAGAGAATGCCAAGGAAATCTGAGTAGTTCATCCTTTCTCCTCCTCTCCCCTTTTAGATAATTTTTGTTCGATTCTTTCTCCACCTTTTCTGGTGGATCATTCTCTTTGAGCTGGTTGTGCCTTTGTTTTATTTACATACATTCATGGAGAGTTTTCTTTATTCTATTTAGATTGTATAGTAGCCAGGCATGTTCACTGTACTGTATTCGATTTTCATGGCTTTTTGAGGGATTGGTTTGTAAAAGGAGCCAAGAATTAGCGAACCTAGATTCTCATAGGTTTGATGTTTTGGTTAATTTATAATTTTAGTACTGACTGTACCTACGTATTTTTATCTACACTGTGTCAGAAATCCTAGATGATAAGCCGTGATGTTCGATGCAATAACGTTTTATCAAGGTTGAAATTTTGAAAGAAGACCAATT

mRNA sequence

ATAGAATATAGAATATAGATCCCATTCCCACCAGCGACAAGTTGGCAATGGCACCGATATGAAGACGGCGATGTATACAACAGCGTCCAGTCGAACCCAACGTCCTTTTCTATATTTAGGAGTCATCTTCCATTCCATTTTCCTTTCCATATTTGGTCTTCTTCAATCCTCCGCCCATTCTTAAATCTTCAACAACCTTCCTCTCCTCTATAACTATCCCTTCCTTTCTCTTCCTCAATCCACATCCAATTTCATCAGCACCAGCCCCCAGGAGACGCAATTTCTTCCGGAGCATCTTCTGGGAGCAACCCCCACCCGGGGAATCACTCACAAACACAATGCCAGGTGGTGAAGAGAGAGATGATGGTGTTATGGCCACTGACTTCTTCTGGTCTTACACCGATGAACCTCACGCTTCCAGGAGACGACAAATTCTCTCTCAGTACCCTCAGATCCGGGAGCTCTTCGGTCCTGACCCCTGGGCTTTCTTCAAGATTTCTGTGGTTGTTCTGCTTCAGTTATGGACTGCTACGACCCTTCACAGTGCAGACTGGTTAAAGATATTGGCAATAGCCTACTTCTTTGGCTCTTTTCTCAACCACAACCTATTTTTAGCCATTCACGAGCTCAGTCACAACCTTGCCTTCTCAACACCTGATTACAACAAGTGGCTCGGGATTTTTGCTAATCTCCCCATTGGAGTGCCCATGTCCATCACATTTCAAAAGTATCACCTTGAGCACCATCGCTTTCAAGGAGTGGATGGCATGGATATGGACGTTCCAAGCCAGACCGAAGCTCATCTGGTGACTAATGTTGTCTCAAAAGCTATATGGGTTATCTTCCAACTATTCTTCTATGCACTCCGCCCACTGTTTCTCAAACCAAAGCCCCCAGGTCACTGGGAGTTCATCAACTTCATTATTCAGATAGCCCTTGATGTTACCATGGTTTACTTCTGGAGTTGGAAGTCTTTTGCTTACTTGATCCTCTCCACATTCGTTGGCGGTGGGATGCATCCAATGGCTGGTCACTTCATTTCCGAACATTATGTCTTCAATCCCAAACAGGAAACATATTCTTACTACGGTCCACTTAATCTTCTTACATGGAGTGTAGGTTACCACAATGAACACCATGACTTCCCCAGAATTCCAGGAAGCAAGCTTTATAAAGTGAAGGAGATTGCACCTGAATACTACAATGAATTAGATTCATACAAATCGTGGAGCCAGGTGATATACATGTACATTATGGACAAGACAGTGGGGCCATTCAGTAGGATGAAGAGAATGCCAAGGAAATCTGAGTAGTTCATCCTTTCTCCTCCTCTCCCCTTTTAGATAATTTTTGTTCGATTCTTTCTCCACCTTTTCTGGTGGATCATTCTCTTTGAGCTGGTTGTGCCTTTGTTTTATTTACATACATTCATGGAGAGTTTTCTTTATTCTATTTAGATTGTATAGTAGCCAGGCATGTTCACTGTACTGTATTCGATTTTCATGGCTTTTTGAGGGATTGGTTTGTAAAAGGAGCCAAGAATTAGCGAACCTAGATTCTCATAGGTTTGATGTTTTGGTTAATTTATAATTTTAGTACTGACTGTACCTACGTATTTTTATCTACACTGTGTCAGAAATCCTAGATGATAAGCCGTGATGTTCGATGCAATAACGTTTTATCAAGGTTGAAATTTTGAAAGAAGACCAATT

Coding sequence (CDS)

ATGCCAGGTGGTGAAGAGAGAGATGATGGTGTTATGGCCACTGACTTCTTCTGGTCTTACACCGATGAACCTCACGCTTCCAGGAGACGACAAATTCTCTCTCAGTACCCTCAGATCCGGGAGCTCTTCGGTCCTGACCCCTGGGCTTTCTTCAAGATTTCTGTGGTTGTTCTGCTTCAGTTATGGACTGCTACGACCCTTCACAGTGCAGACTGGTTAAAGATATTGGCAATAGCCTACTTCTTTGGCTCTTTTCTCAACCACAACCTATTTTTAGCCATTCACGAGCTCAGTCACAACCTTGCCTTCTCAACACCTGATTACAACAAGTGGCTCGGGATTTTTGCTAATCTCCCCATTGGAGTGCCCATGTCCATCACATTTCAAAAGTATCACCTTGAGCACCATCGCTTTCAAGGAGTGGATGGCATGGATATGGACGTTCCAAGCCAGACCGAAGCTCATCTGGTGACTAATGTTGTCTCAAAAGCTATATGGGTTATCTTCCAACTATTCTTCTATGCACTCCGCCCACTGTTTCTCAAACCAAAGCCCCCAGGTCACTGGGAGTTCATCAACTTCATTATTCAGATAGCCCTTGATGTTACCATGGTTTACTTCTGGAGTTGGAAGTCTTTTGCTTACTTGATCCTCTCCACATTCGTTGGCGGTGGGATGCATCCAATGGCTGGTCACTTCATTTCCGAACATTATGTCTTCAATCCCAAACAGGAAACATATTCTTACTACGGTCCACTTAATCTTCTTACATGGAGTGTAGGTTACCACAATGAACACCATGACTTCCCCAGAATTCCAGGAAGCAAGCTTTATAAAGTGAAGGAGATTGCACCTGAATACTACAATGAATTAGATTCATACAAATCGTGGAGCCAGGTGATATACATGTACATTATGGACAAGACAGTGGGGCCATTCAGTAGGATGAAGAGAATGCCAAGGAAATCTGAGTAG

Protein sequence

MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKPKPPGHWEFINFIIQIALDVTMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYMYIMDKTVGPFSRMKRMPRKSE*
Homology
[Close]
BLAST of CSPI01G12010 vs. ExPASy Swiss-Prot
Match: Q6H5U3 (Sphingolipid delta(4)-desaturase DES1-like OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0639600 PE=2 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 2.3e-163
Identity = 263/322 (81.68%), Postives = 290/322 (90.06%), Query Frame = 0 

Query: 3   GGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLW 62
           G    ++GVMATDFFWSYTDEPHA+RRR+IL+++PQI+ELFGPDP AF KI+ VV LQLW
Sbjct: 6   GDGREEEGVMATDFFWSYTDEPHATRRREILAKHPQIKELFGPDPLAFLKIAAVVSLQLW 65

Query: 63  TATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPIGV 122
           TAT L  A W+KIL +AYFFGSFLNHNLFLAIHELSHNLAF+TP YN+WLGIFANLPIGV
Sbjct: 66  TATLLRDASWVKILTVAYFFGSFLNHNLFLAIHELSHNLAFTTPSYNRWLGIFANLPIGV 125

Query: 123 PMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLK 182
           PMSITFQKYHLEHHRFQGVDG+DMD+PSQ EAH V N +SK++WV+FQLFFYALRPLFLK
Sbjct: 126 PMSITFQKYHLEHHRFQGVDGIDMDIPSQAEAHAVKNTLSKSVWVVFQLFFYALRPLFLK 185

Query: 183 PKPPGHWEFINFIIQIALDVTMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNP 242
           PKPPG WEF N IIQIALD +MVYF+ WKS AYLILSTFVGGGMHPMAGHFISEHYVFNP
Sbjct: 186 PKPPGLWEFTNLIIQIALDASMVYFFGWKSLAYLILSTFVGGGMHPMAGHFISEHYVFNP 245

Query: 243 KQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIY 302
            QETYSYYGPLNL+TW VGYHNEHHDFPRIPG++LYKV+EIAPEYYN L SYKSWSQVIY
Sbjct: 246 DQETYSYYGPLNLMTWHVGYHNEHHDFPRIPGTRLYKVREIAPEYYNNLKSYKSWSQVIY 305

Query: 303 MYIMDKTVGPFSRMKRMPRKSE 325
           MYIMD+TVGPFSRMKR   K +
Sbjct: 306 MYIMDQTVGPFSRMKRKAPKKD 327

[Close]
BLAST of CSPI01G12010 vs. ExPASy Swiss-Prot
Match: Q9ZPH4 (Sphingolipid delta(4)-desaturase DES1-like OS=Arabidopsis thaliana OX=3702 GN=At4g04930 PE=2 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 1.9e-162
Identity = 266/320 (83.12%), Postives = 293/320 (91.56%), Query Frame = 0 

Query: 5   EERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLWTA 64
           EE  +GVMATDFFWSYTDEPHASRRRQILS YPQIR+LFGPDPWAF KI++VV+LQL TA
Sbjct: 13  EEEREGVMATDFFWSYTDEPHASRRRQILSCYPQIRQLFGPDPWAFLKITLVVILQLSTA 72

Query: 65  TTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPIGVPM 124
             LH++ WLKIL+IAYFFGSFLNHNLFLAIHELSHNLAFSTP YN+ LGIFANLPIGVPM
Sbjct: 73  AILHNSGWLKILSIAYFFGSFLNHNLFLAIHELSHNLAFSTPVYNRCLGIFANLPIGVPM 132

Query: 125 SITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKPK 184
           S+TFQKYHLEHHRFQGVDG+DMDVP+ TEAHLVTN+ +K IWV  QLFFYALRP+F+KPK
Sbjct: 133 SVTFQKYHLEHHRFQGVDGIDMDVPTYTEAHLVTNIFAKTIWVFLQLFFYALRPIFIKPK 192

Query: 185 PPGHWEFINFIIQIALDVTMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQ 244
           PPG+WEFINF+IQI LDV++V F+ W+SFAYLILSTFVGGGMHPMAGHFISEHYVFNP Q
Sbjct: 193 PPGYWEFINFLIQIVLDVSVVLFFGWRSFAYLILSTFVGGGMHPMAGHFISEHYVFNPNQ 252

Query: 245 ETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYMY 304
           ETYSYYGPLNLLTWSVGYHNEHHDFPRIPG+KL+ VKEIA EYY  L+SYKSWSQVIYMY
Sbjct: 253 ETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLHLVKEIAGEYYEGLESYKSWSQVIYMY 312

Query: 305 IMDKTVGPFSRMKRMPRKSE 325
           IMD TVGP+SRMKR   KS+
Sbjct: 313 IMDTTVGPYSRMKRKLSKSD 332

[Close]
BLAST of CSPI01G12010 vs. ExPASy Swiss-Prot
Match: Q6UQ04 (Sphingolipid delta(4)-desaturase DES1 OS=Microtus fortis OX=100897 GN=DEGS1 PE=2 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 1.4e-96
Identity = 159/316 (50.32%), Postives = 222/316 (70.25%), Query Frame = 0 

Query: 11  VMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLWTATTLHSA 70
           V   +F W YTD+PH  RRR+IL++YP+I+ L  PDP   + I+ + L+QL +   +   
Sbjct: 5   VSREEFEWVYTDQPHTDRRREILAKYPEIKSLMKPDPNLIWIITAMFLVQLISLYLVKDL 64

Query: 71  DWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFS--TPDYNKWLGIFANLPIGVPMSITF 130
           DW  ++  +Y FGS LNH++ LAIHE+SHN  F      +N+W GIFANLPIGVP S++F
Sbjct: 65  DWKWVIFWSYVFGSCLNHSMTLAIHEISHNFPFGHHKAVWNRWFGIFANLPIGVPYSVSF 124

Query: 131 QKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKPKPPGH 190
           ++YH++HHR+ G DG+D+D+P+  E         K +WVI Q  FYALRPLF+ PKP  +
Sbjct: 125 KRYHMDHHRYLGADGIDVDIPTDFEGWFFCTTFRKLVWVILQPLFYALRPLFINPKPITY 184

Query: 191 WEFINFIIQIALDVTMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYS 250
            E IN + QI LD+ + Y +  KS  Y++ ++ +G G+HP++GHFI+EHY+F    ETYS
Sbjct: 185 LEIINTVTQIILDIIVYYVFGIKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYS 244

Query: 251 YYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYMYIMDK 310
           YYGPLNLLT++VGYHNEHHDFP +PG  L  V++IA EYY++L  Y SW +V+Y ++MD 
Sbjct: 245 YYGPLNLLTFNVGYHNEHHDFPNVPGKNLPLVRKIAAEYYDKLPQYNSWIKVLYDFVMDD 304

Query: 311 TVGPFSRMKRMPRKSE 325
           T+ P+SRMKR P+ SE
Sbjct: 305 TISPYSRMKRPPKGSE 320

[Close]
BLAST of CSPI01G12010 vs. ExPASy Swiss-Prot
Match: O15121 (Sphingolipid delta(4)-desaturase DES1 OS=Homo sapiens OX=9606 GN=DEGS1 PE=1 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 5.9e-95
Identity = 158/310 (50.97%), Postives = 216/310 (69.68%), Query Frame = 0 

Query: 11  VMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLWTATTLHSA 70
           V   DF W YTD+PHA RRR+IL++YP+I+ L  PDP   + I ++VL QL     +   
Sbjct: 5   VSREDFEWVYTDQPHADRRREILAKYPEIKSLMKPDPNLIWIIIMMVLTQLGAFYIVKDL 64

Query: 71  DWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFST--PDYNKWLGIFANLPIGVPMSITF 130
           DW  ++  AY FGS +NH++ LAIHE++HN AF      +N+W G+FANLPIG+P SI+F
Sbjct: 65  DWKWVIFGAYAFGSCINHSMTLAIHEIAHNAAFGNCKAMWNRWFGMFANLPIGIPYSISF 124

Query: 131 QKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKPKPPGH 190
           ++YH++HHR+ G DG+D+D+P+  E         K IWVI Q  FYA RPLF+ PKP  +
Sbjct: 125 KRYHMDHHRYLGADGVDVDIPTDFEGWFFCTAFRKFIWVILQPLFYAFRPLFINPKPITY 184

Query: 191 WEFINFIIQIALDVTMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYS 250
            E IN + Q+  D+ + YF   KS  Y++ ++ +G G+HP++GHFI+EHY+F    ETYS
Sbjct: 185 LEVINTVAQVTFDILIYYFLGIKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYS 244

Query: 251 YYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYMYIMDK 310
           YYGPLNLLT++VGYHNEHHDFP IPG  L  V++IA EYY+ L  Y SW +V+Y ++MD 
Sbjct: 245 YYGPLNLLTFNVGYHNEHHDFPNIPGKSLPLVRKIAAEYYDNLPHYNSWIKVLYDFVMDD 304

Query: 311 TVGPFSRMKR 319
           T+ P+SRMKR
Sbjct: 305 TISPYSRMKR 314

[Close]
BLAST of CSPI01G12010 vs. ExPASy Swiss-Prot
Match: Q5XIF5 (Sphingolipid delta(4)-desaturase DES1 OS=Rattus norvegicus OX=10116 GN=Degs1 PE=2 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 7.7e-95
Identity = 155/316 (49.05%), Postives = 223/316 (70.57%), Query Frame = 0 

Query: 11  VMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLWTATTLHSA 70
           V   +F W YTD+PHA+RR++IL++YP+I+ L  PDP   + ++ ++L+QL +   +   
Sbjct: 5   VSREEFEWVYTDQPHAARRQEILAKYPEIKSLMKPDPNLIWIVTSMLLVQLASFYLVKDL 64

Query: 71  DWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFS--TPDYNKWLGIFANLPIGVPMSITF 130
           DW  ++  +Y FGS LNH++ LAIHE+SHN  F      +N+W G+FANL +GVP SI+F
Sbjct: 65  DWKWLMFWSYAFGSCLNHSMTLAIHEISHNFPFGHHKAMWNRWFGMFANLSLGVPYSISF 124

Query: 131 QKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKPKPPGH 190
           ++YH++HHR+ G DG+D+D+P+  E       + K +WVI Q  FYA RPLF+ PKP  H
Sbjct: 125 KRYHMDHHRYLGADGIDVDIPTDFEGWFFCTTLRKLVWVILQPLFYAFRPLFINPKPITH 184

Query: 191 WEFINFIIQIALDVTMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYS 250
            E IN +IQ+  DV + Y +  KS  Y++ ++ +G G+HP++GHFI+EHY+F    ETYS
Sbjct: 185 LEVINTVIQVTFDVLVYYVFGVKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYS 244

Query: 251 YYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYMYIMDK 310
           YYGPLNLLT++VGYHNEHHDFP +PG  L  V++IA EYY+ L  Y SW +V+Y ++MD 
Sbjct: 245 YYGPLNLLTFNVGYHNEHHDFPNVPGKNLPLVRKIASEYYDNLPHYNSWIRVLYDFVMDD 304

Query: 311 TVGPFSRMKRMPRKSE 325
           T+ P+SRMKR P+ +E
Sbjct: 305 TISPYSRMKRPPKGNE 320

[Close]
BLAST of CSPI01G12010 vs. ExPASy TrEMBL
Match: A0A0A0LSB9 (Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis sativus OX=3659 GN=Csa_1G073750 PE=3 SV=1)

HSP 1 Score: 686.0 bits (1769), Expect = 7.5e-194
Identity = 323/324 (99.69%), Postives = 323/324 (99.69%), Query Frame = 0 

Query: 1   MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60
           MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ
Sbjct: 1   MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60

Query: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120
           LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVTMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQIALDV MVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMDKTVGPFSRMKRMPRKSE
Sbjct: 301 IYMYIMDKTVGPFSRMKRMPRKSE 324

[Close]
BLAST of CSPI01G12010 vs. ExPASy TrEMBL
Match: A0A5D3DSS5 (Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00680 PE=3 SV=1)

HSP 1 Score: 677.9 bits (1748), Expect = 2.0e-191
Identity = 319/324 (98.46%), Postives = 321/324 (99.07%), Query Frame = 0 

Query: 1   MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60
           M GG ERDDGVMATDFFWSYTDEPHASRRRQILS+YPQIRELFGPDPWAFFKIS+VVLLQ
Sbjct: 1   MQGGAERDDGVMATDFFWSYTDEPHASRRRQILSKYPQIRELFGPDPWAFFKISMVVLLQ 60

Query: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120
           LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVTMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQIALDV MVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMDKTVGPFSRMKRMPRKSE
Sbjct: 301 IYMYIMDKTVGPFSRMKRMPRKSE 324

[Close]
BLAST of CSPI01G12010 vs. ExPASy TrEMBL
Match: A0A1S3B5L3 (Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis melo OX=3656 GN=LOC103486276 PE=3 SV=1)

HSP 1 Score: 677.9 bits (1748), Expect = 2.0e-191
Identity = 319/324 (98.46%), Postives = 321/324 (99.07%), Query Frame = 0 

Query: 1   MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60
           M GG ERDDGVMATDFFWSYTDEPHASRRRQILS+YPQIRELFGPDPWAFFKIS+VVLLQ
Sbjct: 1   MQGGAERDDGVMATDFFWSYTDEPHASRRRQILSKYPQIRELFGPDPWAFFKISMVVLLQ 60

Query: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120
           LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVTMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQIALDV MVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMDKTVGPFSRMKRMPRKSE
Sbjct: 301 IYMYIMDKTVGPFSRMKRMPRKSE 324

[Close]
BLAST of CSPI01G12010 vs. ExPASy TrEMBL
Match: A0A6J1GTF2 (Sphingolipid delta(4)-desaturase DES1-like OS=Cucurbita moschata OX=3662 GN=LOC111457308 PE=3 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 2.6e-186
Identity = 307/324 (94.75%), Postives = 318/324 (98.15%), Query Frame = 0 

Query: 1   MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60
           MP GEERD+GVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKI+VVVLLQ
Sbjct: 1   MPRGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKIAVVVLLQ 60

Query: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120
           LWTATTLHSADWLKILA+AYFFGSFLNHNLFLAIHELSHNLAFSTPD+N+WLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAVAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPS TEA LVTN+VSKAIWV+FQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSTTEARLVTNIVSKAIWVVFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVTMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQI LD TMVYFWSWK+FAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIGLDFTMVYFWSWKTFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPE+YNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEFYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMD+TVGPFSRMKRM +KSE
Sbjct: 301 IYMYIMDRTVGPFSRMKRMAKKSE 324

[Close]
BLAST of CSPI01G12010 vs. ExPASy TrEMBL
Match: A0A6J1K2T6 (Sphingolipid delta(4)-desaturase DES1-like OS=Cucurbita maxima OX=3661 GN=LOC111490149 PE=3 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 4.9e-185
Identity = 305/324 (94.14%), Postives = 316/324 (97.53%), Query Frame = 0 

Query: 1   MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60
           MP GEERD+GVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKI+VVVLLQ
Sbjct: 1   MPRGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKIAVVVLLQ 60

Query: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120
           LWTATTLHSADWLKILA+AYFFGSFLNHNLFLAIHELSHNLAFSTPD+N+WLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAVAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPS TEA LVTN+V KAIWV+FQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSMTEARLVTNIVLKAIWVVFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVTMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQI LD TMVY WSWK+FAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIGLDFTMVYLWSWKTFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPE+YNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEFYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMD+TVGPFSRMKRM +KSE
Sbjct: 301 IYMYIMDRTVGPFSRMKRMAKKSE 324

[Close]
BLAST of CSPI01G12010 vs. NCBI nr
Match: XP_004146595.1 (sphingolipid delta(4)-desaturase DES1-like [Cucumis sativus] >KGN64668.1 hypothetical protein Csa_014335 [Cucumis sativus])

HSP 1 Score: 686.0 bits (1769), Expect = 1.5e-193
Identity = 323/324 (99.69%), Postives = 323/324 (99.69%), Query Frame = 0 

Query: 1   MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60
           MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ
Sbjct: 1   MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60

Query: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120
           LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVTMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQIALDV MVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMDKTVGPFSRMKRMPRKSE
Sbjct: 301 IYMYIMDKTVGPFSRMKRMPRKSE 324

[Close]
BLAST of CSPI01G12010 vs. NCBI nr
Match: XP_008442400.1 (PREDICTED: sphingolipid delta(4)-desaturase DES1-like [Cucumis melo] >KAA0056978.1 sphingolipid delta(4)-desaturase DES1-like [Cucumis melo var. makuwa] >TYK26405.1 sphingolipid delta(4)-desaturase DES1-like [Cucumis melo var. makuwa])

HSP 1 Score: 677.9 bits (1748), Expect = 4.2e-191
Identity = 319/324 (98.46%), Postives = 321/324 (99.07%), Query Frame = 0 

Query: 1   MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60
           M GG ERDDGVMATDFFWSYTDEPHASRRRQILS+YPQIRELFGPDPWAFFKIS+VVLLQ
Sbjct: 1   MQGGAERDDGVMATDFFWSYTDEPHASRRRQILSKYPQIRELFGPDPWAFFKISMVVLLQ 60

Query: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120
           LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVTMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQIALDV MVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMDKTVGPFSRMKRMPRKSE
Sbjct: 301 IYMYIMDKTVGPFSRMKRMPRKSE 324

[Close]
BLAST of CSPI01G12010 vs. NCBI nr
Match: XP_038893930.1 (sphingolipid delta(4)-desaturase DES1-like [Benincasa hispida])

HSP 1 Score: 670.6 bits (1729), Expect = 6.7e-189
Identity = 315/324 (97.22%), Postives = 319/324 (98.46%), Query Frame = 0 

Query: 1   MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60
           M GGEERD+GVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ
Sbjct: 1   MQGGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60

Query: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120
           LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYN+WLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNRWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPS TEAHLVTNVVSKAIWV+FQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSLTEAHLVTNVVSKAIWVVFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVTMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPG WEFINFIIQIALDV MVY WSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGQWEFINFIIQIALDVAMVYLWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMD+TVGPFSRMKRMPRKSE
Sbjct: 301 IYMYIMDRTVGPFSRMKRMPRKSE 324

[Close]
BLAST of CSPI01G12010 vs. NCBI nr
Match: KAG7012427.1 (Sphingolipid delta(4)-desaturase DES1-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 661.4 bits (1705), Expect = 4.1e-186
Identity = 307/324 (94.75%), Postives = 318/324 (98.15%), Query Frame = 0 

Query: 1   MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60
           MP GEERD+GVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKI+VVVLLQ
Sbjct: 1   MPRGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKIAVVVLLQ 60

Query: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120
           LWTATTLHSADWLKILA+AYFFGSFLNHNLFLAIHELSHNLAFSTPD+N+WLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAVAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPS TEA LVTN+VSKAIWV+FQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSMTEARLVTNIVSKAIWVVFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVTMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQI LD TMVYFWSWK+FAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIGLDFTMVYFWSWKTFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPE+YNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEFYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMD+TVGPFSRMKRM +KSE
Sbjct: 301 IYMYIMDRTVGPFSRMKRMAKKSE 324

[Close]
BLAST of CSPI01G12010 vs. NCBI nr
Match: XP_022955306.1 (sphingolipid delta(4)-desaturase DES1-like [Cucurbita moschata] >KAG6573258.1 Sphingolipid delta(4)-desaturase DES1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 661.0 bits (1704), Expect = 5.3e-186
Identity = 307/324 (94.75%), Postives = 318/324 (98.15%), Query Frame = 0 

Query: 1   MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60
           MP GEERD+GVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKI+VVVLLQ
Sbjct: 1   MPRGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKIAVVVLLQ 60

Query: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120
           LWTATTLHSADWLKILA+AYFFGSFLNHNLFLAIHELSHNLAFSTPD+N+WLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAVAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPS TEA LVTN+VSKAIWV+FQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSTTEARLVTNIVSKAIWVVFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVTMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQI LD TMVYFWSWK+FAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIGLDFTMVYFWSWKTFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPE+YNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEFYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMD+TVGPFSRMKRM +KSE
Sbjct: 301 IYMYIMDRTVGPFSRMKRMAKKSE 324

[Close]
BLAST of CSPI01G12010 vs. TAIR 10
Match: AT4G04930.1 (fatty acid desaturase family protein )

HSP 1 Score: 573.2 bits (1476), Expect = 1.4e-163
Identity = 266/320 (83.12%), Postives = 293/320 (91.56%), Query Frame = 0 

Query: 5   EERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLWTA 64
           EE  +GVMATDFFWSYTDEPHASRRRQILS YPQIR+LFGPDPWAF KI++VV+LQL TA
Sbjct: 13  EEEREGVMATDFFWSYTDEPHASRRRQILSCYPQIRQLFGPDPWAFLKITLVVILQLSTA 72

Query: 65  TTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPIGVPM 124
             LH++ WLKIL+IAYFFGSFLNHNLFLAIHELSHNLAFSTP YN+ LGIFANLPIGVPM
Sbjct: 73  AILHNSGWLKILSIAYFFGSFLNHNLFLAIHELSHNLAFSTPVYNRCLGIFANLPIGVPM 132

Query: 125 SITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKPK 184
           S+TFQKYHLEHHRFQGVDG+DMDVP+ TEAHLVTN+ +K IWV  QLFFYALRP+F+KPK
Sbjct: 133 SVTFQKYHLEHHRFQGVDGIDMDVPTYTEAHLVTNIFAKTIWVFLQLFFYALRPIFIKPK 192

Query: 185 PPGHWEFINFIIQIALDVTMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQ 244
           PPG+WEFINF+IQI LDV++V F+ W+SFAYLILSTFVGGGMHPMAGHFISEHYVFNP Q
Sbjct: 193 PPGYWEFINFLIQIVLDVSVVLFFGWRSFAYLILSTFVGGGMHPMAGHFISEHYVFNPNQ 252

Query: 245 ETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYMY 304
           ETYSYYGPLNLLTWSVGYHNEHHDFPRIPG+KL+ VKEIA EYY  L+SYKSWSQVIYMY
Sbjct: 253 ETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLHLVKEIAGEYYEGLESYKSWSQVIYMY 312

Query: 305 IMDKTVGPFSRMKRMPRKSE 325
           IMD TVGP+SRMKR   KS+
Sbjct: 313 IMDTTVGPYSRMKRKLSKSD 332

The following BLAST results are available for this feature:
BLAST of CSPI01G12010 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q6H5U32.3e-16381.68Sphingolipid delta(4)-desaturase DES1-like OS=Oryza sativa subsp. japonica OX=39... [more]
Q9ZPH41.9e-16283.13Sphingolipid delta(4)-desaturase DES1-like OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Q6UQ041.4e-9650.32Sphingolipid delta(4)-desaturase DES1 OS=Microtus fortis OX=100897 GN=DEGS1 PE=2... [more]
O151215.9e-9550.97Sphingolipid delta(4)-desaturase DES1 OS=Homo sapiens OX=9606 GN=DEGS1 PE=1 SV=1[more]
Q5XIF57.7e-9549.05Sphingolipid delta(4)-desaturase DES1 OS=Rattus norvegicus OX=10116 GN=Degs1 PE=... [more]
BLAST of CSPI01G12010 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LSB97.5e-19499.69Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis sativus OX=3659 GN=Csa_1G0... [more]
A0A5D3DSS52.0e-19198.46Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3B5L32.0e-19198.46Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis melo OX=3656 GN=LOC1034862... [more]
A0A6J1GTF22.6e-18694.75Sphingolipid delta(4)-desaturase DES1-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1K2T64.9e-18594.14Sphingolipid delta(4)-desaturase DES1-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
BLAST of CSPI01G12010 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004146595.11.5e-19399.69sphingolipid delta(4)-desaturase DES1-like [Cucumis sativus] >KGN64668.1 hypothe... [more]
XP_008442400.14.2e-19198.46PREDICTED: sphingolipid delta(4)-desaturase DES1-like [Cucumis melo] >KAA0056978... [more]
XP_038893930.16.7e-18997.22sphingolipid delta(4)-desaturase DES1-like [Benincasa hispida][more]
KAG7012427.14.1e-18694.75Sphingolipid delta(4)-desaturase DES1-like protein [Cucurbita argyrosperma subsp... [more]
XP_022955306.15.3e-18694.75sphingolipid delta(4)-desaturase DES1-like [Cucurbita moschata] >KAG6573258.1 Sp... [more]
BLAST of CSPI01G12010 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G04930.11.4e-16383.13fatty acid desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013866Sphingolipid delta4-desaturase, N-terminalSMARTSM01269Lipid_DES_2coord: 11..49
e-value: 7.2E-21
score: 85.3
IPR013866Sphingolipid delta4-desaturase, N-terminalPFAMPF08557Lipid_DEScoord: 14..48
e-value: 6.7E-20
score: 70.3
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 72..290
e-value: 1.7E-20
score: 74.0
IPR011388Sphingolipid delta4-desaturasePIRSFPIRSF017228Sphnglp_dlt4_descoord: 4..324
e-value: 4.0E-149
score: 494.1
IPR011388Sphingolipid delta4-desaturaseCDDcd03508Delta4-sphingolipid-FADS-likecoord: 32..318
e-value: 2.05928E-169
score: 470.203
NoneNo IPR availablePANTHERPTHR12879SPHINGOLIPID DELTA 4 DESATURASE/C-4 HYDROXYLASE PROTEIN DES2coord: 5..322
NoneNo IPR availablePANTHERPTHR12879:SF17SPHINGOLIPID DELTA(4)-DESATURASE DES1-LIKEcoord: 5..322

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G12010.1CSPI01G12010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046513 ceramide biosynthetic process
biological_process GO:0006629 lipid metabolic process
biological_process GO:0030148 sphingolipid biosynthetic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042284 sphingolipid delta-4 desaturase activity