CSPI01G11850 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G11850
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	polyphenol oxidase, chloroplastic-like
Location	Chr1: 7466034 .. 7467259 (-)
RNA-Seq Expression	CSPI01G11850
Synteny	CSPI01G11850

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTACTGTTTGGACTTGGAGGGCTTCACGGCGTCACGACTCTTAGCAGCGATAACTTTATTTGCATTGGCGGATTCGCTGTTGTCGCCGGATGTTCTTAACTGCGTGACAAAAGGAAAACACCAGCAGGGGACGATGGATGTTGCCCACCTGAAATCGAAGAAATTATTGAGTTTGTCCCCCCGGTCATTCCAACACCCCGTATCCGACCCGCTGCCCATACAATGACCACAACCCAGCTTGATGATTTAAAAAAAGGGATTGAAAAGATGAGAGCCCTTAGTGACGATGACCCTCGTAGTTTGTATCAACAAGCTAATGTCCACTGTGCCCATTGCAATGGTGCATCCTTCTGAATCAGTAGCTAAAGTTCAAGTTCATAGCAACGCTTTATTCTTCCCACCTCACCGTGCTTATCTTTACTTCTTTGAGAGATTTTTAAGTTCTTTGATTGGGAACCCAGATTTTGCTATACCTTTTTGGAATTGGGATACACCTGATGGCATGTACAATATGACTGAACCCTACAACGACCCGAATTCATCGCTTTACGATAAGTTCCGCGATCCACTTCTCATGTTAAAAAAAAATTGCTTTATCTTAACTACGACAGAAAAACCGAAGACCATGGTTTCGACATTGTTGAATGTAAACTCCATTAGATGAAACAACGAATGAATGTTACGAGCGCCCGAAGGTTCCTTGGAAATATAGTTGAAGTTGGGAAGGTCCCCACCCACAATGACATGGGTAGCACATTGAACAATCTCCACATAATAAGTTCACAATTGGGTTGGCAATAAAAACATACGTGGTCGTATAGACATGGGAGATTTTTCGACCGCAGGACGAGATCCATTGTTTTACGCTCATCACGTGAATGTCGATGGCATGTGGAAGATTTGGAAAGAAATGGGGAATCAAGATCACACCGACGAGGGTTGGGTAAACTCGACATTTGTGTTTTACGATGAGAATCAAAACGCTGTACGTATCAAAGTTTCGGATTGTTTGGACAGTAAGAAACTTGGGTATGATTATGAAAAGGTACCCATTTCATGGAAGCATTTGAAGAAAACCCCGTTACCAAATAATAATTGTATAATTAATAGAGAATTATTCCAGCTGCCTCCAATGAAGCAAAGTTTCCTGTGAAGATGAAACCTAAGCTTGGTGGAGAAAGCGTCAGTATTGGAGGGATAACGGCTCAGCTTCAGCCTGTGTAA

mRNA sequence

ATGTACTGTTTGGACTTGGAGGGCTTCACGGCGTCACGACTCTTAGCAGCGATAACTTTATTTGCATTGGCGGATTCGCTGTTGTCGCCGGATGTTCTTAACTGCGTGACAAAAGGAAAACACCAGCAGGGGACGATGGATGTTGCCCACCTGAAATCGAAGAAATTATTGAGTTTGTCCCCCCGGTCATTCCAACACCCCCTAATGTCCACTGTGCCCATTGCAATGGTGCATCCTTCTGAATCAGTAGCTAAAGTTCAAGTTCATAGCAACGCTTTATTCTTCCCACCTCACCGTGCTTATCTTTACTTCTTTGAGAGATTTTTAAGTTCTTTGATTGGGAACCCAGATTTTGCTATACCTTTTTGGAATTGGGATACACCTGATGGCATGTACAATATGACTGAACCCTACAACGACCCGAATTCATCGCTTTACGATAAGTTCCGCGATCCACTTCTCATCGCCCGAAGGTTCCTTGGAAATATAGTTGAAGTTGGGAAGGTCCCCACCCACAATGACATGGGACGAGATCCATTGTTTTACGCTCATCACGTGAATGTCGATGGCATGTGGAAGATTTGGAAAGAAATGGGGAATCAAGATCACACCGACGAGGGTTGGGTAAACTCGACATTTGTGTTTTACGATGAGAATCAAAACGCTGTACGTATCAAAGTTTCGGATTGTTTGGACAGTAAGAAACTTGGGTATGATTATGAAAAGAGAATTATTCCAGCTGCCTCCAATGAAGCAAAGTTTCCTGTGAAGATGAAACCTAAGCTTGGTGGAGAAAGCGTCAGTATTGGAGGGATAACGGCTCAGCTTCAGCCTGTGTAA

Coding sequence (CDS)

ATGTACTGTTTGGACTTGGAGGGCTTCACGGCGTCACGACTCTTAGCAGCGATAACTTTATTTGCATTGGCGGATTCGCTGTTGTCGCCGGATGTTCTTAACTGCGTGACAAAAGGAAAACACCAGCAGGGGACGATGGATGTTGCCCACCTGAAATCGAAGAAATTATTGAGTTTGTCCCCCCGGTCATTCCAACACCCCCTAATGTCCACTGTGCCCATTGCAATGGTGCATCCTTCTGAATCAGTAGCTAAAGTTCAAGTTCATAGCAACGCTTTATTCTTCCCACCTCACCGTGCTTATCTTTACTTCTTTGAGAGATTTTTAAGTTCTTTGATTGGGAACCCAGATTTTGCTATACCTTTTTGGAATTGGGATACACCTGATGGCATGTACAATATGACTGAACCCTACAACGACCCGAATTCATCGCTTTACGATAAGTTCCGCGATCCACTTCTCATCGCCCGAAGGTTCCTTGGAAATATAGTTGAAGTTGGGAAGGTCCCCACCCACAATGACATGGGACGAGATCCATTGTTTTACGCTCATCACGTGAATGTCGATGGCATGTGGAAGATTTGGAAAGAAATGGGGAATCAAGATCACACCGACGAGGGTTGGGTAAACTCGACATTTGTGTTTTACGATGAGAATCAAAACGCTGTACGTATCAAAGTTTCGGATTGTTTGGACAGTAAGAAACTTGGGTATGATTATGAAAAGAGAATTATTCCAGCTGCCTCCAATGAAGCAAAGTTTCCTGTGAAGATGAAACCTAAGCTTGGTGGAGAAAGCGTCAGTATTGGAGGGATAACGGCTCAGCTTCAGCCTGTGTAA

Protein sequence

MYCLDLEGFTASRLLAAITLFALADSLLSPDVLNCVTKGKHQQGTMDVAHLKSKKLLSLSPRSFQHPLMSTVPIAMVHPSESVAKVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAIPFWNWDTPDGMYNMTEPYNDPNSSLYDKFRDPLLIARRFLGNIVEVGKVPTHNDMGRDPLFYAHHVNVDGMWKIWKEMGNQDHTDEGWVNSTFVFYDENQNAVRIKVSDCLDSKKLGYDYEKRIIPAASNEAKFPVKMKPKLGGESVSIGGITAQLQPV*

Homology

[Close]

BLAST of CSPI01G11850 vs. ExPASy Swiss-Prot
Match: Q9ZP19 (Polyphenol oxidase I, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-1 PE=1 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 6.3e-37
Identity = 94/269 (34.94%), Postives = 128/269 (47.58%), Query Frame = 0


Query: 85  KVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAIPFWNWDTPDGMYNMTEPYNDPNSS 144
           ++QVH++ LFFP HR YLYF+ER L  LIG+P F +PFWNWD P GM  + +  ND  SS
Sbjct: 105 EIQVHNSWLFFPFHRWYLYFYERILGKLIGDPSFGLPFWNWDNPGGMV-LPDFLNDSTSS 164

Query: 145 LYDKFRD-----PLLI----------------------------------ARRFLGNIVE 204
           LYD  R+     P+++                                  A  FLG    
Sbjct: 165 LYDSNRNQSHLPPVVVDLGYNGADTDVTDQQRITDNLALMYKQMVTNAGTAELFLGKAYR 224

Query: 205 VGKVP------------------------THND-------MGRDPLFYAHHVNVDGMWKI 264
            G  P                        T+N+        GRD  FY HH NVD MW I
Sbjct: 225 AGDAPSPGAGSIETSPHIPIHRWVGDPRNTNNEDMGNFYSAGRDIAFYCHHSNVDRMWTI 284

Query: 265 WKEMG----NQDHTDEGWVNSTFVFYDENQNAVRIKVSDCLDSKKLGYDYEKRIIPAASN 280
           W+++      +D+TD  W+N+TF+FYDEN  AV++++ D LD++K+GY Y K  +P   +
Sbjct: 285 WQQLAGKPRKRDYTDSDWLNATFLFYDENGQAVKVRIGDSLDNQKMGYKYAKTPLPWLDS 344

[Close]

BLAST of CSPI01G11850 vs. ExPASy Swiss-Prot
Match: Q9MB14 (Polyphenol oxidase II, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-2 PE=2 SV=2)

HSP 1 Score: 154.8 bits (390), Expect = 1.4e-36
Identity = 95/258 (36.82%), Postives = 126/258 (48.84%), Query Frame = 0


Query: 85  KVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAIPFWNWDTPDGMYNMTEPYNDPNSS 144
           ++QVH++ LFFP HR YLYF+ER L  LIG+P F +PFWNWDTP GM  + + + + NS 
Sbjct: 195 EIQVHNSWLFFPFHRWYLYFYERILGKLIGDPTFGLPFWNWDTPAGML-IPQYFRNQNSP 254

Query: 145 LYDKFRD----PLLI----------------------------------ARRFL------ 204
           LYD+ R+    PL++                                  A  FL      
Sbjct: 255 LYDENRNQSHLPLVMDLGYAGTDTDVTDQERISNNLALMYKSMVTNAGTAELFLGKPYKA 314

Query: 205 ---------GNIVEVGKVPTH-------------NDM------GRDPLFYAHHVNVDGMW 264
                    G+I  +   P H              DM      GRD LFY HH NVD MW
Sbjct: 315 GDDPVNKGGGSIENIPHTPVHRWVGDVKPRTQNGEDMGNFYSAGRDILFYCHHSNVDRMW 374

Query: 265 KIWKEMG----NQDHTDEGWVNSTFVFYDENQNAVRIKVSDCLDSKKLGYDYEKRIIPAA 267
            IW+++G     +D TD  W+++TF+FYDEN+ AVR++V D LD++KLGY YE   +P  
Sbjct: 375 TIWQQLGGKGRRRDFTDSDWLDATFIFYDENKQAVRVRVGDALDNQKLGYKYEFTNLPWL 434

[Close]

BLAST of CSPI01G11850 vs. ExPASy Swiss-Prot
Match: P43309 (Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 4.1e-36
Identity = 90/239 (37.66%), Postives = 116/239 (48.54%), Query Frame = 0


Query: 85  KVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAIPFWNWDTPDGMYNMTEPYNDPNSS 144
           ++Q+H++ LFFP HR YLYFFE+ L  LI +P FA+PFWNWD+P GM  +   Y DP S 
Sbjct: 192 ELQIHNSWLFFPFHRYYLYFFEKILGKLINDPTFALPFWNWDSPAGM-PLPAIYADPKSP 251

Query: 145 LYDKFRD-----PLLI----------------------------------ARRFLGNIVE 204
           LYDK R      P L+                                  A+ F GN   
Sbjct: 252 LYDKLRSANHQPPTLVDLDYNGTEDNVSKETTINANLKIMYRQMVSNSKNAKLFFGNPYR 311

Query: 205 VG-------------------------KVPTHNDM------GRDPLFYAHHVNVDGMWKI 252
            G                           P   DM      GRDP+F+AHH NVD MW I
Sbjct: 312 AGDEPDPGGGSIEGTPHAPVHLWTGDNTQPNFEDMGNFYSAGRDPIFFAHHSNVDRMWSI 371

[Close]

BLAST of CSPI01G11850 vs. ExPASy Swiss-Prot
Match: Q06215 (Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 5.3e-36
Identity = 98/250 (39.20%), Postives = 123/250 (49.20%), Query Frame = 0


Query: 85  KVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAIPFWNWDTPDGMYNMTEPYNDPNSS 144
           K+QVH + LFFP HR YLYF+ER L SLI +P FA+PFWN+D PDGM  +   Y D  S 
Sbjct: 198 KLQVHGSWLFFPFHRWYLYFYERILGSLINDPTFALPFWNYDAPDGM-QLPTIYADKASP 257

Query: 145 LYDKFRD-----PLLI----------------------------------ARRFLGN--- 204
           LYD+ R+     P LI                                  +R FLGN   
Sbjct: 258 LYDELRNASHQPPTLIDLNFCDIGSDIDRNELIKTNLSIMYRQVYSNGKTSRLFLGNPYR 317

Query: 205 -----------IVEVGKVPTH-----------NDMG------RDPLFYAHHVNVDGMWKI 263
                      I  V   P H            DMG      RDP+FY+HH NVD +W I
Sbjct: 318 AGDAEPQGAGSIENVPHAPVHTWTGDNTQTNIEDMGIFYSAARDPIFYSHHSNVDRLWYI 377

[Close]

BLAST of CSPI01G11850 vs. ExPASy Swiss-Prot
Match: P43311 (Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 8.5e-34
Identity = 92/256 (35.94%), Postives = 118/256 (46.09%), Query Frame = 0


Query: 85  KVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAIPFWNWDTPDGMYNMTEPYNDPNSS 144
           ++QVH++ LF P HR YLYF ER L+ LI +P FA+P+W WD PDGMY M   Y    SS
Sbjct: 207 ELQVHASWLFLPFHRYYLYFNERILAKLIDDPTFALPYWAWDNPDGMY-MPTIYASSPSS 266

Query: 145 LYDKFRD-----PLLI----------------------------------ARRFLGNIVE 204
           LYD+ R+     P +I                                   + FLG    
Sbjct: 267 LYDEKRNAKHLPPTVIDLDYDGTEPTIPDDELKTDNLAIMYKQIVSGATTPKLFLGYPYR 326

Query: 205 VGKV----------PTHN--------------DM------GRDPLFYAHHVNVDGMWKIW 264
            G              HN              DM      GRDP+F+ HH NVD MW IW
Sbjct: 327 AGDAIDPGAGTLEHAPHNIVHKWTGLADKPSEDMGNFYTAGRDPIFFGHHANVDRMWNIW 386

Query: 265 KEMG---NQDHTDEGWVNSTFVFYDENQNAVRIKVSDCLDSKKLGYDYEKRIIPAASNEA 269
           K +G    +D TD  W+++TFVFYDEN+  V++KVSDC+D+ KL Y Y+   IP      
Sbjct: 387 KTIGGKNRKDFTDTDWLDATFVFYDENKQLVKVKVSDCVDTSKLRYQYQDIPIPWLPKNT 446

[Close]

BLAST of CSPI01G11850 vs. ExPASy TrEMBL
Match: A0A0A0LRV2 (Tyrosinase_Cu-bd domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G073100 PE=4 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 1.3e-157
Identity = 278/304 (91.45%), Postives = 279/304 (91.78%), Query Frame = 0


Query: 1   MYCLDLEGFTASRLLAAITLFALADSLLSPDVLNCVTKGKHQQGTMDVAHLKSKKLLSLS 60
           MYCLDLEGFTASRLLAAITLFALADSLLSPDVLNCVTKGKHQQGTMDVAHLKSKKLLSLS
Sbjct: 1   MYCLDLEGFTASRLLAAITLFALADSLLSPDVLNCVTKGKHQQGTMDVAHLKSKKLLSLS 60

Query: 61  PRSFQHPLMSTVPIAMVHPSESVAKVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAI 120
           PRSFQHPLMSTVPIAM+HPSESVAKVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAI
Sbjct: 61  PRSFQHPLMSTVPIAMMHPSESVAKVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAI 120

Query: 121 PFWNWDTPDGMYNMTEPYNDPNSSLYDKFRDPLLI------------------------- 180
           PFWNWDTPDGMYNMTEPYNDPNSSLYDKFRDPLLI                         
Sbjct: 121 PFWNWDTPDGMYNMTEPYNDPNSSLYDKFRDPLLIKTEDHGFDIVECKLHWMKQRMNVTS 180

Query: 181 ARRFLGNIVEVGKVPTHNDMGRDPLFYAHHVNVDGMWKIWKEMGNQDHTDEGWVNSTFVF 240
           ARRFLGNIVEVGKVPTHNDMGRDPLFYAHHVNVDGMWKIWKEMGNQDHTDEGWVNSTFVF
Sbjct: 181 ARRFLGNIVEVGKVPTHNDMGRDPLFYAHHVNVDGMWKIWKEMGNQDHTDEGWVNSTFVF 240

Query: 241 YDENQNAVRIKVSDCLDSKKLGYDYEKRIIPAASNEAKFPVKMKPKLGGESVSIGGITAQ 280
           YDENQNAVRIKVSDCLDSKKLGYDYEKRIIPAASNEAKFPVKMKPKLGGESVSIGGITAQ
Sbjct: 241 YDENQNAVRIKVSDCLDSKKLGYDYEKRIIPAASNEAKFPVKMKPKLGGESVSIGGITAQ 300

[Close]

BLAST of CSPI01G11850 vs. ExPASy TrEMBL
Match: A0A5A7UM88 (Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00520 PE=3 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 9.1e-47
Identity = 116/275 (42.18%), Postives = 143/275 (52.00%), Query Frame = 0


Query: 52  KSKKLLSLSPRSFQHPLMSTVPIAMVH-----------PSESVAKVQVHSNALFFPPHRA 111
           K K+L    P SF H        A +H            S +  + +VHSNALFFP HRA
Sbjct: 140 KLKQLDEDDPHSFSHQ-------AKIHCAYCDYAYNQLGSITETRFEVHSNALFFPFHRA 199

Query: 112 YLYFFERFLSSLIGNPDFAIPFWNWDTPDGMYNMTEPYNDPNSSLYDKFRDP-------- 171
           YLYFFER L   IGNPDFAIPFWNWD+P GM+ M E YN P+SSLYD  RDP        
Sbjct: 200 YLYFFERILRHYIGNPDFAIPFWNWDSPPGMH-MPEFYNRPSSSLYDNLRDPRHDPHKLI 259

Query: 172 ----------------------------LLIARRFLGNIVEVGKVPTHNDMGR------- 231
                                       +   R FLG +++ G+ P  +DMG        
Sbjct: 260 DLNYNGKTEDHSFDVVDCNLRWMNDRMKVQSPRSFLGRVLKAGETPKPSDMGTIEQSPHN 319

Query: 232 ------------------------DPLFYAHHVNVDGMWKIWKEM-GN-QDHTDEGWVNS 247
                                   DPLFYAHH N+D +W IWK + GN +DHTD  W+NS
Sbjct: 320 NIHDWVGTKTPHFEDMGSFYSAALDPLFYAHHTNIDRLWNIWKTLDGNPKDHTDPDWLNS 379

[Close]

BLAST of CSPI01G11850 vs. ExPASy TrEMBL
Match: A0A1S3BFJ5 (polyphenol oxidase I, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103489300 PE=3 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 9.1e-47
Identity = 116/275 (42.18%), Postives = 143/275 (52.00%), Query Frame = 0


Query: 52  KSKKLLSLSPRSFQHPLMSTVPIAMVH-----------PSESVAKVQVHSNALFFPPHRA 111
           K K+L    P SF H        A +H            S +  + +VHSNALFFP HRA
Sbjct: 305 KLKQLDEDDPHSFSHQ-------AKIHCAYCDYAYNQLGSITETRFEVHSNALFFPFHRA 364

Query: 112 YLYFFERFLSSLIGNPDFAIPFWNWDTPDGMYNMTEPYNDPNSSLYDKFRDP-------- 171
           YLYFFER L   IGNPDFAIPFWNWD+P GM+ M E YN P+SSLYD  RDP        
Sbjct: 365 YLYFFERILRHYIGNPDFAIPFWNWDSPPGMH-MPEFYNRPSSSLYDNLRDPRHDPHKLI 424

Query: 172 ----------------------------LLIARRFLGNIVEVGKVPTHNDMGR------- 231
                                       +   R FLG +++ G+ P  +DMG        
Sbjct: 425 DLNYNGKTEDHSFDVVDCNLRWMNDRMKVQSPRSFLGRVLKAGETPKPSDMGTIEQSPHN 484

Query: 232 ------------------------DPLFYAHHVNVDGMWKIWKEM-GN-QDHTDEGWVNS 247
                                   DPLFYAHH N+D +W IWK + GN +DHTD  W+NS
Sbjct: 485 NIHDWVGTKTPHFEDMGSFYSAALDPLFYAHHTNIDRLWNIWKTLDGNPKDHTDPDWLNS 544

[Close]

BLAST of CSPI01G11850 vs. ExPASy TrEMBL
Match: A0A0A0LUI1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G073090 PE=3 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 1.0e-45
Identity = 105/228 (46.05%), Postives = 125/228 (54.82%), Query Frame = 0


Query: 88  VHSNALFFPPHRAYLYFFERFLSSLIGNPDFAIPFWNWDTPDGMYNMTEPYNDPNSSLYD 147
           VHSNALFFP HRAYLYFFER L   IGNP+FAIPFWNWD+P GMY M E YN P+SSLYD
Sbjct: 179 VHSNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMY-MPEFYNRPSSSLYD 238

Query: 148 KFRDP------------------------------------LLIARRFLGNIVEVGKVPT 207
             RDP                                    +   R FLG +++ G+ P 
Sbjct: 239 HLRDPRHDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQVQSPRSFLGRVLKAGETPN 298

Query: 208 HNDMGR-------------------------------DPLFYAHHVNVDGMWKIWKEM-G 247
             DMG                                DPLFYAHH N+D +W IWK + G
Sbjct: 299 PTDMGTIEQSPHNNIHDWLGTKTPHFEDMGSFYSAALDPLFYAHHANIDRLWNIWKTLDG 358

[Close]

BLAST of CSPI01G11850 vs. ExPASy TrEMBL
Match: A0A5A7UM24 (Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G001410 PE=3 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 3.6e-43
Identity = 121/297 (40.74%), Postives = 142/297 (47.81%), Query Frame = 0


Query: 27  LLSPDVLNCVTKGKHQQGTMDVAHLKSKKLLSLSPRSFQHPLMSTVPIAMVH-------- 86
           L++PD +  + KG              K L    PRSF H        A VH        
Sbjct: 129 LMTPDQIADLEKGIQMM----------KMLPEDDPRSFAHQ-------AKVHCAYCDDAY 188

Query: 87  -PSESVAKVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAIPFWNWDTPDGMYNMTEP 146
              +S    QVH NALFFP HRAYLYFFER L  L+  PDFAIPFWNWD P GM  M + 
Sbjct: 189 KQMDSTMDFQVHENALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMC-MPKI 248

Query: 147 YNDPNSSLYDKFRD-----PLLI------------------------------ARRFLGN 206
           Y  P+SSLYD+ RD     P L+                              AR FLG 
Sbjct: 249 YTKPSSSLYDENRDECHQPPKLLNLNYGGKKVKDSEIVDFNLRWMNDQMSVETAREFLGK 308

Query: 207 IVEVGKVP----------THNDM----------------------GRDPLFYAHHVNVDG 247
           +++ G  P           HN++                      GRDPLFYAHH NVD 
Sbjct: 309 VIKRGNCPENGSGSIETSPHNNLHNWVGDPEATIHGEDMGHFYSAGRDPLFYAHHANVDR 368

[Close]

BLAST of CSPI01G11850 vs. NCBI nr
Match: KAE8652707.1 (hypothetical protein Csa_013729 [Cucumis sativus])

HSP 1 Score: 318.2 bits (814), Expect = 7.3e-83
Identity = 153/158 (96.84%), Postives = 157/158 (99.37%), Query Frame = 0


Query: 1   MYCLDLEGFTASRLLAAITLFALADSLLSPDVLNCVTKGKHQQGTMDVAHLKSKKLLSLS 60
           MYCLDLEGFTASRLLAAITLFALADSLLSPDVLNCVTKGKHQQGTMDVAHLKSKKLLSLS
Sbjct: 1   MYCLDLEGFTASRLLAAITLFALADSLLSPDVLNCVTKGKHQQGTMDVAHLKSKKLLSLS 60

Query: 61  PRSFQHPLMSTVPIAMVHPSESVAKVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAI 120
           PRSFQHPLMSTVPIAM+HPSESVAKVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAI
Sbjct: 61  PRSFQHPLMSTVPIAMMHPSESVAKVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAI 120

Query: 121 PFWNWDTPDGMYNMTEPYNDPNSSLYDKFRDPLLIARR 159
           PFWNWDTPDGMYNMTEPYNDPNSSLYDKFRDPLL+ ++
Sbjct: 121 PFWNWDTPDGMYNMTEPYNDPNSSLYDKFRDPLLMLKK 158

[Close]

BLAST of CSPI01G11850 vs. NCBI nr
Match: XP_011648445.1 (polyphenol oxidase, chloroplastic [Cucumis sativus] >KAE8652706.1 hypothetical protein Csa_014130 [Cucumis sativus])

HSP 1 Score: 224.2 bits (570), Expect = 1.4e-54
Identity = 104/104 (100.00%), Postives = 104/104 (100.00%), Query Frame = 0


Query: 176 GRDPLFYAHHVNVDGMWKIWKEMGNQDHTDEGWVNSTFVFYDENQNAVRIKVSDCLDSKK 235
           GRDPLFYAHHVNVDGMWKIWKEMGNQDHTDEGWVNSTFVFYDENQNAVRIKVSDCLDSKK
Sbjct: 8   GRDPLFYAHHVNVDGMWKIWKEMGNQDHTDEGWVNSTFVFYDENQNAVRIKVSDCLDSKK 67

Query: 236 LGYDYEKRIIPAASNEAKFPVKMKPKLGGESVSIGGITAQLQPV 280
           LGYDYEKRIIPAASNEAKFPVKMKPKLGGESVSIGGITAQLQPV
Sbjct: 68  LGYDYEKRIIPAASNEAKFPVKMKPKLGGESVSIGGITAQLQPV 111

[Close]

BLAST of CSPI01G11850 vs. NCBI nr
Match: XP_008446626.1 (PREDICTED: polyphenol oxidase I, chloroplastic-like [Cucumis melo])

HSP 1 Score: 197.2 bits (500), Expect = 1.9e-46
Identity = 116/275 (42.18%), Postives = 143/275 (52.00%), Query Frame = 0


Query: 52  KSKKLLSLSPRSFQHPLMSTVPIAMVH-----------PSESVAKVQVHSNALFFPPHRA 111
           K K+L    P SF H        A +H            S +  + +VHSNALFFP HRA
Sbjct: 305 KLKQLDEDDPHSFSHQ-------AKIHCAYCDYAYNQLGSITETRFEVHSNALFFPFHRA 364

Query: 112 YLYFFERFLSSLIGNPDFAIPFWNWDTPDGMYNMTEPYNDPNSSLYDKFRDP-------- 171
           YLYFFER L   IGNPDFAIPFWNWD+P GM+ M E YN P+SSLYD  RDP        
Sbjct: 365 YLYFFERILRHYIGNPDFAIPFWNWDSPPGMH-MPEFYNRPSSSLYDNLRDPRHDPHKLI 424

Query: 172 ----------------------------LLIARRFLGNIVEVGKVPTHNDMGR------- 231
                                       +   R FLG +++ G+ P  +DMG        
Sbjct: 425 DLNYNGKTEDHSFDVVDCNLRWMNDRMKVQSPRSFLGRVLKAGETPKPSDMGTIEQSPHN 484

Query: 232 ------------------------DPLFYAHHVNVDGMWKIWKEM-GN-QDHTDEGWVNS 247
                                   DPLFYAHH N+D +W IWK + GN +DHTD  W+NS
Sbjct: 485 NIHDWVGTKTPHFEDMGSFYSAALDPLFYAHHTNIDRLWNIWKTLDGNPKDHTDPDWLNS 544

[Close]

BLAST of CSPI01G11850 vs. NCBI nr
Match: KAA0056962.1 (polyphenol oxidase I [Cucumis melo var. makuwa] >TYK26390.1 polyphenol oxidase I [Cucumis melo var. makuwa])

HSP 1 Score: 197.2 bits (500), Expect = 1.9e-46
Identity = 116/275 (42.18%), Postives = 143/275 (52.00%), Query Frame = 0


Query: 52  KSKKLLSLSPRSFQHPLMSTVPIAMVH-----------PSESVAKVQVHSNALFFPPHRA 111
           K K+L    P SF H        A +H            S +  + +VHSNALFFP HRA
Sbjct: 140 KLKQLDEDDPHSFSHQ-------AKIHCAYCDYAYNQLGSITETRFEVHSNALFFPFHRA 199

Query: 112 YLYFFERFLSSLIGNPDFAIPFWNWDTPDGMYNMTEPYNDPNSSLYDKFRDP-------- 171
           YLYFFER L   IGNPDFAIPFWNWD+P GM+ M E YN P+SSLYD  RDP        
Sbjct: 200 YLYFFERILRHYIGNPDFAIPFWNWDSPPGMH-MPEFYNRPSSSLYDNLRDPRHDPHKLI 259

Query: 172 ----------------------------LLIARRFLGNIVEVGKVPTHNDMGR------- 231
                                       +   R FLG +++ G+ P  +DMG        
Sbjct: 260 DLNYNGKTEDHSFDVVDCNLRWMNDRMKVQSPRSFLGRVLKAGETPKPSDMGTIEQSPHN 319

Query: 232 ------------------------DPLFYAHHVNVDGMWKIWKEM-GN-QDHTDEGWVNS 247
                                   DPLFYAHH N+D +W IWK + GN +DHTD  W+NS
Sbjct: 320 NIHDWVGTKTPHFEDMGSFYSAALDPLFYAHHTNIDRLWNIWKTLDGNPKDHTDPDWLNS 379

[Close]

BLAST of CSPI01G11850 vs. NCBI nr
Match: XP_004146641.1 (polyphenol oxidase, chloroplastic [Cucumis sativus] >KGN64649.1 hypothetical protein Csa_013698 [Cucumis sativus])

HSP 1 Score: 193.7 bits (491), Expect = 2.1e-45
Identity = 105/228 (46.05%), Postives = 125/228 (54.82%), Query Frame = 0


Query: 88  VHSNALFFPPHRAYLYFFERFLSSLIGNPDFAIPFWNWDTPDGMYNMTEPYNDPNSSLYD 147
           VHSNALFFP HRAYLYFFER L   IGNP+FAIPFWNWD+P GMY M E YN P+SSLYD
Sbjct: 179 VHSNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMY-MPEFYNRPSSSLYD 238

Query: 148 KFRDP------------------------------------LLIARRFLGNIVEVGKVPT 207
             RDP                                    +   R FLG +++ G+ P 
Sbjct: 239 HLRDPRHDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQVQSPRSFLGRVLKAGETPN 298

Query: 208 HNDMGR-------------------------------DPLFYAHHVNVDGMWKIWKEM-G 247
             DMG                                DPLFYAHH N+D +W IWK + G
Sbjct: 299 PTDMGTIEQSPHNNIHDWLGTKTPHFEDMGSFYSAALDPLFYAHHANIDRLWNIWKTLDG 358

The following BLAST results are available for this feature:

BLAST of CSPI01G11850 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZP19	6.3e-37	34.94	Polyphenol oxidase I, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-1 PE=1 SV=1	[more]
Q9MB14	1.4e-36	36.82	Polyphenol oxidase II, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-2 PE=2 SV=...	[more]
P43309	4.1e-36	37.66	Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1	[more]
Q06215	5.3e-36	39.20	Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1	[more]
P43311	8.5e-34	35.94	Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1	[more]

BLAST of CSPI01G11850 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LRV2	1.3e-157	91.45	Tyrosinase_Cu-bd domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G0...	[more]
A0A5A7UM88	9.1e-47	42.18	Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861...	[more]
A0A1S3BFJ5	9.1e-47	42.18	polyphenol oxidase I, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103489300...	[more]
A0A0A0LUI1	1.0e-45	46.05	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G073090 PE=3 SV=1	[more]
A0A5A7UM24	3.6e-43	40.74	Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G...	[more]

BLAST of CSPI01G11850 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8652707.1	7.3e-83	96.84	hypothetical protein Csa_013729 [Cucumis sativus]	[more]
XP_011648445.1	1.4e-54	100.00	polyphenol oxidase, chloroplastic [Cucumis sativus] >KAE8652706.1 hypothetical p...	[more]
XP_008446626.1	1.9e-46	42.18	PREDICTED: polyphenol oxidase I, chloroplastic-like [Cucumis melo]	[more]
KAA0056962.1	1.9e-46	42.18	polyphenol oxidase I [Cucumis melo var. makuwa] >TYK26390.1 polyphenol oxidase I...	[more]
XP_004146641.1	2.1e-45	46.05	polyphenol oxidase, chloroplastic [Cucumis sativus] >KGN64649.1 hypothetical pro...	[more]

BLAST of CSPI01G11850 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR022739	Polyphenol oxidase, central domain	PFAM	PF12142	PPO1_DWL	coord: 202..251 e-value: 3.8E-19 score: 68.2
IPR002227	Tyrosinase copper-binding domain	PFAM	PF00264	Tyrosinase	coord: 75..135 e-value: 1.0E-12 score: 48.8 coord: 176..197 e-value: 5.4E-6 score: 26.8
IPR002227	Tyrosinase copper-binding domain	PROSITE	PS00498	TYROSINASE_2	coord: 178..189
IPR008922	Uncharacterised domain, di-copper centre	GENE3D	1.10.1280.10		coord: 171..244 e-value: 1.4E-21 score: 79.4
IPR008922	Uncharacterised domain, di-copper centre	GENE3D	1.10.1280.10		coord: 51..170 e-value: 2.5E-20 score: 75.2
IPR008922	Uncharacterised domain, di-copper centre	SUPERFAMILY	48056	Di-copper centre-containing domain	coord: 70..241
None	No IPR available	PANTHER	PTHR11474	TYROSINASE FAMILY MEMBER	coord: 85..150
None	No IPR available	PANTHER	PTHR11474:SF87	POLYPHENOL OXIDASE, CHLOROPLASTIC-LIKE	coord: 175..252 coord: 85..150
None	No IPR available	PANTHER	PTHR11474	TYROSINASE FAMILY MEMBER	coord: 175..252

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G11850.1	CSPI01G11850.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004097	catechol oxidase activity
molecular_function	GO:0016491	oxidoreductase activity