CSPI01G11840 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G11840
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionpolyphenol oxidase I, chloroplastic-like
LocationChr1: 7462934 .. 7464610 (-)
RNA-Seq ExpressionCSPI01G11840
SyntenyCSPI01G11840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCTTTATCTTCTCTTCTATTACTTTCTTCCTATTCTCAAACCAAAACCAAATCCTCCATTTCTTCAAACAAAAGACATGTTGTTGTCAATTATGTCTCATGCAAAGCAGCCTCTAATAATGGAGAAGATTCTGTAAGCAAATTTGATCGTAGAGAAGTGTTGTTCGGTCTTGGAGGTCTTTATGGCGTCGTCACGGCCACCAGCTCTGTGTTGGCAGCTCCATTGACGCCAGATATCTTTAACTGTCATATGGCGAAAACACCTGGTGGCATCAAGGATTGTTGCCCACCACAAATTGGAAAAATCATCCATTTCGTCCCCCCAAAAATTCAACAACCTTATGTTCGACGACCCGCTGCCCATTTGATGAACCAGACCCAAATTGAGGAATTGGAAAGTGGCATTAAGAAATTAAAACAACTCGATGAAGGTGACCCGCATAGCTTTTTACATCAAGCTAAAATTCATTGTGCATATTGCGATTATGTGTACAACCAGTTGGGGTCTATCACAGAAACTAGATTTGGAGTTCATAGCAACGCGTTATTCTTCCCATTTCATCGTGCCTATCTCTACTTCTTCGAGAGAATCTTGAGGCATTATATTGGGAACCCAAATTTCGCCATACCCTTTTGGAATTGGGATTCACCACCGGGGATGTACATGCCCGAATTCTACAACCGCCCATCTTCATCACTTTACGATCATCTCCGTGATCCACGTCACGATCCACACAAATTGATTTACCTAAACTACAATGGAAAAACCGAAGACCATTCTTTTGACGTTGTGGATTGCAATCTCCGTTGGATGAATGACCGAATGCAAGTCCAAAGCCCTCGAAGTTTCCTTGGAAGAGTTCTGAAAGCTGGAGAAACACCCAATCCCACTGATATGGGCACCATTGAACAATCCCCACATAATAACATTCACGATTGGCTTGGCACAAAGACCCCTCATTTTGAAGACATGGGATCTTTTTACTCGGCAGCGCTAGATCCGTTGTTTTATGCTCACCATGCCAATATAGACCGGTTGTGGAATATTTGGAAAACATTGGACGGGAATCCAAAAGATCACATTGACCCAGATTGGTTGAATTCCTCGTTTGTGTTTTACGATGAGAATAAAAACGCTGTAAATATCAAAGTTTCTGATTGTTTGGACACTGAAAAGCTTGGTTATGTTTACCAAGAGGTACCACTTCCATGGTTGCACATGAACAAAATCCCCACCCCAAATCCGTGGATAAGAACTGAAGAATCTGAGCCTCCAATCGTCAACTTCCCCCAGATTTTGAATTCGGACATAACCACAATGGTAGACAGGCCGGAAGAGGATAGGAGCGGTGAGGAAGTTGATGAGGTGTTGGTGTTGGAAGATGTGGAGTATGATCCAACTACTCGGGTGCATTTTGATGTACTTGTTAATGTTGTTGATAAGGCGCAGGACAGGTGCGCTGCGTTTCTTGAGTTTGTGGGCAGCTTCAAAAACATTCCCCATGGTCTCCACCACCATGATAAAACCTCTTTGCGTTTTGTGATAACTGAGGTCATTCAAGGGTTGGGTTTGGAGCGTGATAAAAATTTGGCTGTTAAGATTGAACCTATGGTTGGTGGAGAAGGTGTCACCGTTGGAGGAATTAAGATTCAACTTGAGCCCCTTCGATAA

mRNA sequence

ATGGCTTCTTTATCTTCTCTTCTATTACTTTCTTCCTATTCTCAAACCAAAACCAAATCCTCCATTTCTTCAAACAAAAGACATGTTGTTGTCAATTATGTCTCATGCAAAGCAGCCTCTAATAATGGAGAAGATTCTGTAAGCAAATTTGATCGTAGAGAAGTGTTGTTCGGTCTTGGAGGTCTTTATGGCGTCGTCACGGCCACCAGCTCTGTGTTGGCAGCTCCATTGACGCCAGATATCTTTAACTGTCATATGGCGAAAACACCTGGTGGCATCAAGGATTGTTGCCCACCACAAATTGGAAAAATCATCCATTTCGTCCCCCCAAAAATTCAACAACCTTATGTTCGACGACCCGCTGCCCATTTGATGAACCAGACCCAAATTGAGGAATTGGAAAGTGGCATTAAGAAATTAAAACAACTCGATGAAGGTGACCCGCATAGCTTTTTACATCAAGCTAAAATTCATTGTGCATATTGCGATTATGTGTACAACCAGTTGGGGTCTATCACAGAAACTAGATTTGGAGTTCATAGCAACGCGTTATTCTTCCCATTTCATCGTGCCTATCTCTACTTCTTCGAGAGAATCTTGAGGCATTATATTGGGAACCCAAATTTCGCCATACCCTTTTGGAATTGGGATTCACCACCGGGGATGTACATGCCCGAATTCTACAACCGCCCATCTTCATCACTTTACGATCATCTCCGTGATCCACGTCACGATCCACACAAATTGATTTACCTAAACTACAATGGAAAAACCGAAGACCATTCTTTTGACGTTGTGGATTGCAATCTCCGTTGGATGAATGACCGAATGCAAGTCCAAAGCCCTCGAAGTTTCCTTGGAAGAGTTCTGAAAGCTGGAGAAACACCCAATCCCACTGATATGGGCACCATTGAACAATCCCCACATAATAACATTCACGATTGGCTTGGCACAAAGACCCCTCATTTTGAAGACATGGGATCTTTTTACTCGGCAGCGCTAGATCCGTTGTTTTATGCTCACCATGCCAATATAGACCGGTTGTGGAATATTTGGAAAACATTGGACGGGAATCCAAAAGATCACATTGACCCAGATTGGTTGAATTCCTCGTTTGTGTTTTACGATGAGAATAAAAACGCTGTAAATATCAAAGTTTCTGATTGTTTGGACACTGAAAAGCTTGGTTATGTTTACCAAGAGGTACCACTTCCATGGTTGCACATGAACAAAATCCCCACCCCAAATCCGTGGATAAGAACTGAAGAATCTGAGCCTCCAATCGTCAACTTCCCCCAGATTTTGAATTCGGACATAACCACAATGGTAGACAGGCCGGAAGAGGATAGGAGCGGTGAGGAAGTTGATGAGGTGTTGGTGTTGGAAGATGTGGAGTATGATCCAACTACTCGGGTGCATTTTGATGTACTTGTTAATGTTGTTGATAAGGCGCAGGACAGGTGCGCTGCGTTTCTTGAGTTTGTGGGCAGCTTCAAAAACATTCCCCATGGTCTCCACCACCATGATAAAACCTCTTTGCGTTTTGTGATAACTGAGGTCATTCAAGGGTTGGGTTTGGAGCGTGATAAAAATTTGGCTGTTAAGATTGAACCTATGGTTGGTGGAGAAGGTGTCACCGTTGGAGGAATTAAGATTCAACTTGAGCCCCTTCGATAA

Coding sequence (CDS)

ATGGCTTCTTTATCTTCTCTTCTATTACTTTCTTCCTATTCTCAAACCAAAACCAAATCCTCCATTTCTTCAAACAAAAGACATGTTGTTGTCAATTATGTCTCATGCAAAGCAGCCTCTAATAATGGAGAAGATTCTGTAAGCAAATTTGATCGTAGAGAAGTGTTGTTCGGTCTTGGAGGTCTTTATGGCGTCGTCACGGCCACCAGCTCTGTGTTGGCAGCTCCATTGACGCCAGATATCTTTAACTGTCATATGGCGAAAACACCTGGTGGCATCAAGGATTGTTGCCCACCACAAATTGGAAAAATCATCCATTTCGTCCCCCCAAAAATTCAACAACCTTATGTTCGACGACCCGCTGCCCATTTGATGAACCAGACCCAAATTGAGGAATTGGAAAGTGGCATTAAGAAATTAAAACAACTCGATGAAGGTGACCCGCATAGCTTTTTACATCAAGCTAAAATTCATTGTGCATATTGCGATTATGTGTACAACCAGTTGGGGTCTATCACAGAAACTAGATTTGGAGTTCATAGCAACGCGTTATTCTTCCCATTTCATCGTGCCTATCTCTACTTCTTCGAGAGAATCTTGAGGCATTATATTGGGAACCCAAATTTCGCCATACCCTTTTGGAATTGGGATTCACCACCGGGGATGTACATGCCCGAATTCTACAACCGCCCATCTTCATCACTTTACGATCATCTCCGTGATCCACGTCACGATCCACACAAATTGATTTACCTAAACTACAATGGAAAAACCGAAGACCATTCTTTTGACGTTGTGGATTGCAATCTCCGTTGGATGAATGACCGAATGCAAGTCCAAAGCCCTCGAAGTTTCCTTGGAAGAGTTCTGAAAGCTGGAGAAACACCCAATCCCACTGATATGGGCACCATTGAACAATCCCCACATAATAACATTCACGATTGGCTTGGCACAAAGACCCCTCATTTTGAAGACATGGGATCTTTTTACTCGGCAGCGCTAGATCCGTTGTTTTATGCTCACCATGCCAATATAGACCGGTTGTGGAATATTTGGAAAACATTGGACGGGAATCCAAAAGATCACATTGACCCAGATTGGTTGAATTCCTCGTTTGTGTTTTACGATGAGAATAAAAACGCTGTAAATATCAAAGTTTCTGATTGTTTGGACACTGAAAAGCTTGGTTATGTTTACCAAGAGGTACCACTTCCATGGTTGCACATGAACAAAATCCCCACCCCAAATCCGTGGATAAGAACTGAAGAATCTGAGCCTCCAATCGTCAACTTCCCCCAGATTTTGAATTCGGACATAACCACAATGGTAGACAGGCCGGAAGAGGATAGGAGCGGTGAGGAAGTTGATGAGGTGTTGGTGTTGGAAGATGTGGAGTATGATCCAACTACTCGGGTGCATTTTGATGTACTTGTTAATGTTGTTGATAAGGCGCAGGACAGGTGCGCTGCGTTTCTTGAGTTTGTGGGCAGCTTCAAAAACATTCCCCATGGTCTCCACCACCATGATAAAACCTCTTTGCGTTTTGTGATAACTGAGGTCATTCAAGGGTTGGGTTTGGAGCGTGATAAAAATTTGGCTGTTAAGATTGAACCTATGGTTGGTGGAGAAGGTGTCACCGTTGGAGGAATTAAGATTCAACTTGAGCCCCTTCGATAA

Protein sequence

MASLSSLLLLSSYSQTKTKSSISSNKRHVVVNYVSCKAASNNGEDSVSKFDRREVLFGLGGLYGVVTATSSVLAAPLTPDIFNCHMAKTPGGIKDCCPPQIGKIIHFVPPKIQQPYVRRPAAHLMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIHCAYCDYVYNQLGSITETRFGVHSNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMYMPEFYNRPSSSLYDHLRDPRHDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQVQSPRSFLGRVLKAGETPNPTDMGTIEQSPHNNIHDWLGTKTPHFEDMGSFYSAALDPLFYAHHANIDRLWNIWKTLDGNPKDHIDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLPWLHMNKIPTPNPWIRTEESEPPIVNFPQILNSDITTMVDRPEEDRSGEEVDEVLVLEDVEYDPTTRVHFDVLVNVVDKAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLERDKNLAVKIEPMVGGEGVTVGGIKIQLEPLR*
Homology
BLAST of CSPI01G11840 vs. ExPASy Swiss-Prot
Match: P43311 (Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 6.1e-116
Identity = 236/567 (41.62%), Postives = 321/567 (56.61%), Query Frame = 0

Query: 24  SNKRHVVVNYVSCKAA---------SNNGEDSVSKFDRREVLFGLGGLYGVVTATSS--- 83
           +  R    + VSC +A         S+  E S  K DRR VL G+GGLYG      +   
Sbjct: 40  NRSRRFAPSKVSCNSANGDPNSDSTSDVRETSSGKLDRRNVLLGIGGLYGAAGGLGATKP 99

Query: 84  -VLAAPL-TPDIFNCHMAKTPGGI--KDCCPPQIGKIIHFVPPKIQQPYVRRPAAHLMNQ 143
               AP+  PDI  C  A  P G+   +CCPP   KII F  P    P   RPAAHL+++
Sbjct: 100 LAFGAPIQAPDISKCGTATVPDGVTPTNCCPPVTTKIIDFQLPSSGSPMRTRPAAHLVSK 159

Query: 144 TQIEELESGIKKLKQLDEGDPHSFLHQAKIHCAYCDYVYNQLGSITETRFGVHSNALFFP 203
             + + +  I+  K L + DP SF  QA +HC YC   Y+Q+G  T+    VH++ LF P
Sbjct: 160 EYLAKYKKAIELQKALPDDDPRSFKQQANVHCTYCQGAYDQVG-YTDLELQVHASWLFLP 219

Query: 204 FHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMYMPEFYNRPSSSLYDHLRDPRHDPH 263
           FHR YLYF ERIL   I +P FA+P+W WD+P GMYMP  Y    SSLYD  R+ +H P 
Sbjct: 220 FHRYYLYFNERILAKLIDDPTFALPYWAWDNPDGMYMPTIYASSPSSLYDEKRNAKHLPP 279

Query: 264 KLIYLNYNG---KTEDHSFDVVDCNLRWMNDRMQVQSPRSFLGRVLKAGETPNPTDMGTI 323
            +I L+Y+G      D      +  + +        +P+ FLG   +AG+  +P   GT+
Sbjct: 280 TVIDLDYDGTEPTIPDDELKTDNLAIMYKQIVSGATTPKLFLGYPYRAGDAIDP-GAGTL 339

Query: 324 EQSPHNNIHDWLGTKTPHFEDMGSFYSAALDPLFYAHHANIDRLWNIWKTLDG-NPKDHI 383
           E +PHN +H W G      EDMG+FY+A  DP+F+ HHAN+DR+WNIWKT+ G N KD  
Sbjct: 340 EHAPHNIVHKWTGLADKPSEDMGNFYTAGRDPIFFGHHANVDRMWNIWKTIGGKNRKDFT 399

Query: 384 DPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLPWLHMNKIPTPNPWIRTEE 443
           D DWL+++FVFYDENK  V +KVSDC+DT KL Y YQ++P+PWL  N         ++ +
Sbjct: 400 DTDWLDATFVFYDENKQLVKVKVSDCVDTSKLRYQYQDIPIPWLPKNTKAKAKTTTKSSK 459

Query: 444 S------EPP------IVNFPQILNSDITTMVDRPEEDRS---GEEVDEVLVLEDVEYDP 503
           S      E P      I +FP+ LNS I   V RP++ RS    E+ +EVL+++ +E D 
Sbjct: 460 SGVAKAAELPKTTISSIGDFPKALNSVIRVEVPRPKKSRSKKEKEDEEEVLLIKGIELDR 519

Query: 504 TTRVHFDVLVNVVDKAQDRCAAFLEFVGSFKNIPHG--LHHHDKTSLRFVITEVIQGLGL 554
              V FDV +N  D +  R     EF GSF N+PH        KT+LRF I E+++ LG 
Sbjct: 520 ENFVKFDVYINDEDYSVSR-PKNSEFAGSFVNVPHKHMKEMKTKTNLRFAINELLEDLGA 579

BLAST of CSPI01G11840 vs. ExPASy Swiss-Prot
Match: P43309 (Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 1.4e-115
Identity = 238/578 (41.18%), Postives = 332/578 (57.44%), Query Frame = 0

Query: 10  LSSYSQTKTKSSISSNKRHVVVNYVSCKAASNNGEDSV-SKFDRREVLFGLGGLYGVVTA 69
           LS +SQ  ++ S+ +  +      VSCKA +N+  D   SK DRR VL GLGGLYGV   
Sbjct: 22  LSPFSQNNSQVSLLTKPKRSFARKVSCKATNNDQNDQAQSKLDRRNVLLGLGGLYGVAGM 81

Query: 70  TSS--VLAAPLT-PDIFNCHMAKTPGGI--KDCCPPQIGKIIHFVPPKIQQPYVRRPAAH 129
            +     A P+  PD+  C  A  P G    +CCPP   KII F  P   +  + RP AH
Sbjct: 82  GTDPFAFAKPIAPPDVSKCGPADLPQGAVPTNCCPPPSTKIIDFKLPAPAKLRI-RPPAH 141

Query: 130 LMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIHCAYCDYVYNQLGSITETRFGVHSNA 189
            ++Q   ++    ++ +K L + DP SF  QA +HCAYCD  Y+Q+G   E    +H++ 
Sbjct: 142 AVDQAYRDKYYKAMELMKALPDDDPRSFKQQAAVHCAYCDGAYDQVG-FPELELQIHNSW 201

Query: 190 LFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMYMPEFYNRPSSSLYDHLRDPR 249
           LFFPFHR YLYFFE+IL   I +P FA+PFWNWDSP GM +P  Y  P S LYD LR   
Sbjct: 202 LFFPFHRYYLYFFEKILGKLINDPTFALPFWNWDSPAGMPLPAIYADPKSPLYDKLRSAN 261

Query: 250 HDPHKLIYLNYNGKTEDHSFD-VVDCNLRWMNDRMQVQSPRS--FLGRVLKAGETPNPTD 309
           H P  L+ L+YNG  ++ S +  ++ NL+ M  +M   S  +  F G   +AG+ P+P  
Sbjct: 262 HQPPTLVDLDYNGTEDNVSKETTINANLKIMYRQMVSNSKNAKLFFGNPYRAGDEPDPGG 321

Query: 310 MGTIEQSPHNNIHDWLGTKT-PHFEDMGSFYSAALDPLFYAHHANIDRLWNIWKTLDGNP 369
            G+IE +PH  +H W G  T P+FEDMG+FYSA  DP+F+AHH+N+DR+W+IWKTL G  
Sbjct: 322 -GSIEGTPHAPVHLWTGDNTQPNFEDMGNFYSAGRDPIFFAHHSNVDRMWSIWKTLGGKR 381

Query: 370 KDHIDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLPWLH----------- 429
            D  D DWL+S F+FY+EN   V +KV DCL+T+ LGYVYQ+V +PWL            
Sbjct: 382 TDLTDSDWLDSGFLFYNENAELVRVKVRDCLETKNLGYVYQDVDIPWLSSKPTPRRAKVA 441

Query: 430 ----MNKIPTPNPWIRTEESEPPIVNFPQILNSDITTMVDRPEE----DRSGEEVDEVLV 489
                 K+   +  + +         FP  L S I+T+V RP++     ++ E+ +E+LV
Sbjct: 442 LSKVAKKLGVAHAAVASSSKVVAGTEFPISLGSKISTVVKRPKQKKRSKKAKEDEEEILV 501

Query: 490 LEDVEYDPTTRVHFDVLVNVVD---KAQDRCAAFLEFVGSFKNIPHGLHHHDK--TSLRF 549
           +E +E+D    V FDV VN VD      D+     EF GSF ++PH   H  K  T LR 
Sbjct: 502 IEGIEFDRDVAVKFDVYVNDVDDLPSGPDK----TEFAGSFVSVPHSHKHKKKMNTILRL 561

Query: 550 VITEVIQGLGLERDKNLAVKIEPMVGGEGVTVGGIKIQ 554
            +T++++ +  E D ++ V + P  G   V +GGIKI+
Sbjct: 562 GLTDLLEEIEAEDDDSVVVTLVPKFG--AVKIGGIKIE 590

BLAST of CSPI01G11840 vs. ExPASy Swiss-Prot
Match: Q6UIL3 ((+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 5.3e-112
Identity = 246/587 (41.91%), Postives = 339/587 (57.75%), Query Frame = 0

Query: 1   MASLS--SLLLLSSYS-QTKTKSSISSNKRHVVVNYVSCKAASN-----NGEDSVSKFDR 60
           MASLS  S LL + YS    TK S  S +R      VSCKA+++     N ++     DR
Sbjct: 1   MASLSSQSKLLATPYSFPYHTKPSRVSLRR------VSCKASNDNKDKPNDQEKTFSIDR 60

Query: 61  REVLFGLGGLYG---VVTATSSVLAAPLTPDIF--NCHMAK--TPGGIKDCCPPQIGKII 120
           R +L GLGGLYG   V  +  S LAAP+ P +   +CH  +    G    CCPP +   I
Sbjct: 61  RNMLIGLGGLYGASNVFPSNQSTLAAPIQPPVLPDSCHPPEDLAEGVNVLCCPPDVKDPI 120

Query: 121 HFVPPKIQQPYVRRPAAHLMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIHCAYCDYV 180
            F  P        RPAAHL + T IE+ +  +  +K L + DP SF  QA IHCAYC+  
Sbjct: 121 DFQMPSNPSRLRIRPAAHLADPTYIEKYKKALAAMKALPQNDPRSFYQQANIHCAYCNGA 180

Query: 181 YNQLGSITETRFGVHSNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMYMP 240
           Y+Q+G   +    VH + LF PFHR YLYF+ERIL   I +P FAIPFWNWD+P GM+MP
Sbjct: 181 YDQVG-FPDVNIQVHHSWLFLPFHRWYLYFYERILGSLIDDPTFAIPFWNWDAPKGMHMP 240

Query: 241 EFYNRPSSSLYDHLRDPRHDPHKLIYLNY-NGKTEDHSFDVVDCNLRWMNDR-MQVQSPR 300
             +  P+S LYD  R+P H P  ++ L++ +G+   H+   +  NL  M  + ++ +  R
Sbjct: 241 HMFIDPNSPLYDAKRNPAHFPDTIVDLDFSSGEAPSHNPRQIGNNLSIMYKQVVRAKKAR 300

Query: 301 SFLGRVLKAGETPNPTDMGTIEQSPHNNIHDWLG-TKTPHFEDMGSFYSAALDPLFYAHH 360
            F GR L+AG  P+ +  G++E +PH NIH W G  +  +FE+MG+FYSA  DPLFYAHH
Sbjct: 301 LFHGRPLQAGSFPDESGDGSLEGTPHGNIHLWSGDPRQSNFENMGNFYSAGRDPLFYAHH 360

Query: 361 ANIDRLWNIWKTLDGNPKDHIDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEV 420
           AN+DR+W IWK   G  KD+   DWLN+ F+ +DEN   V + V D LD  KLGY YQEV
Sbjct: 361 ANVDRMWYIWKNSLGR-KDYKKKDWLNAGFLLFDENAQPVRVYVRDALDERKLGYAYQEV 420

Query: 421 PLPWLHMNKIP-TPNPWIRTEESEPP-----IVNFPQILNSDITTMVDRPEEDRS---GE 480
            +PW++    P   NPW     S+       I  FP  L+S ++  V RP++ RS    E
Sbjct: 421 DIPWINSKPKPRKANPWPNRLRSKATTTTKLINKFPLTLDSTVSFEVKRPKKSRSKSEKE 480

Query: 481 EVDEVLVLEDVEYDPTTRVHFDVLVNVVDKAQDRCAA-FLEFVGSFKNIPHGLHHHDK-- 540
           + +EVLV+E ++++P   + FDV +N  D+ +D  AA   EF GSF N+PH   H DK  
Sbjct: 481 DEEEVLVIEKIKHEPQFPLKFDVYIN--DEDEDPSAADQTEFAGSFVNVPHFHRHGDKKD 540

Query: 541 ----TSLRFVITEVIQGLGLERDKNLAVKIEPMVGGEGVTVGGIKIQ 554
               T+L   I+EV+  L ++ D ++ V + P VG   +T+GG KI+
Sbjct: 541 KRQTTNLSIGISEVLDELDVDGDDSIVVTLVPRVGSGQITIGGAKIE 577

BLAST of CSPI01G11840 vs. ExPASy Swiss-Prot
Match: Q06215 (Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 3.2e-109
Identity = 241/613 (39.31%), Postives = 333/613 (54.32%), Query Frame = 0

Query: 1   MASLSSLLLLSSYSQTKTKSSIS-------------SNKRHVVVNYVSCKAASNNG---- 60
           M S+S+L  +S+ +   + S IS             S+K       V+C + +N      
Sbjct: 1   MTSISALSFISTIN-VSSNSKISHSSVYPFLQKQHQSSKLRKPKRQVTCSSNNNQNNPKE 60

Query: 61  EDSVSKF--DRREVLFGLGGLYGVVTATSSVLAAPLT-PDIFNC------HMAKTPGGIK 120
           E  +S     RR VL GLGG+YG +    S LA+P++ PD+  C          TP  I 
Sbjct: 61  EQELSNIVGHRRNVLIGLGGIYGTLATNPSALASPISPPDLSKCVPPSDLPSGTTPPNI- 120

Query: 121 DCCPPQIGKIIHFVPPKIQQPYVRRPAAHLMNQTQIEELESGIKKLKQLDEGDPHSFLHQ 180
           +CCPP   KI  F  P   QP   R AAHL++   +E+ +   + +K L   DP +F  Q
Sbjct: 121 NCCPPYSTKITDFKFPS-NQPLRVRQAAHLVDNEFLEKYKKATELMKALPSNDPRNFTQQ 180

Query: 181 AKIHCAYCDYVYNQLGSITETRFGVHSNALFFPFHRAYLYFFERILRHYIGNPNFAIPFW 240
           A IHCAYCD  Y+Q+G   + +  VH + LFFPFHR YLYF+ERIL   I +P FA+PFW
Sbjct: 181 ANIHCAYCDGAYSQIG-FPDLKLQVHGSWLFFPFHRWYLYFYERILGSLINDPTFALPFW 240

Query: 241 NWDSPPGMYMPEFYNRPSSSLYDHLRDPRHDPHKLIYLNY--NGKTEDHSFDVVDCNLRW 300
           N+D+P GM +P  Y   +S LYD LR+  H P  LI LN+   G   D + +++  NL  
Sbjct: 241 NYDAPDGMQLPTIYADKASPLYDELRNASHQPPTLIDLNFCDIGSDIDRN-ELIKTNLSI 300

Query: 301 MNDRM--QVQSPRSFLGRVLKAGETPNPTDMGTIEQSPHNNIHDWLGTKT-PHFEDMGSF 360
           M  ++    ++ R FLG   +AG+   P   G+IE  PH  +H W G  T  + EDMG F
Sbjct: 301 MYRQVYSNGKTSRLFLGNPYRAGDA-EPQGAGSIENVPHAPVHTWTGDNTQTNIEDMGIF 360

Query: 361 YSAALDPLFYAHHANIDRLWNIWKTLDGNPKDHIDPDWLNSSFVFYDENKNAVNIKVSDC 420
           YSAA DP+FY+HH+N+DRLW IWKTL G   D  D DWL S F+FYDENKN V + V D 
Sbjct: 361 YSAARDPIFYSHHSNVDRLWYIWKTLGGKKHDFTDKDWLESGFLFYDENKNLVRVNVKDS 420

Query: 421 LDTEKLGYVYQEVPLPWLHMNKIP--------------------TPNPWIRTEESEPPIV 480
           LD +KLGY YQ+VP+PW     +P                    +P  ++  ++S    V
Sbjct: 421 LDIDKLGYAYQDVPIPWEKAKPVPRRTKVQKLVEVEVNDGNLRKSPTIFLVRQQSPRKYV 480

Query: 481 NFPQILNSDITTMVDRPEEDRS---GEEVDEVLVLEDVEYDPTTRVHFDVLVNVVDKAQD 540
            FP +LN+ ++ +V RP++ RS    EE +EVLV+E +E+     + FDV +N  D+   
Sbjct: 481 TFPLVLNNKVSAIVKRPKKLRSKKEKEEEEEVLVIEGIEFYMNIAIKFDVYIN--DEDDK 540

Query: 541 RCAAFLEFVGSFKNIPHGLHHHDK----TSLRFVITEVIQGLGLERDKNLAVKIEPMVGG 556
             A   EF GSF NIPH  H H      TSLR  IT++++ L +E D N+ V + P  G 
Sbjct: 541 VGAGNTEFAGSFVNIPHSAHGHKNKKIITSLRLGITDLLEDLHVEGDDNIVVTLVPKCGS 600

BLAST of CSPI01G11840 vs. ExPASy Swiss-Prot
Match: O81103 (Polyphenol oxidase latent form, chloroplastic OS=Prunus armeniaca OX=36596 GN=PPO PE=1 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 1.2e-108
Identity = 239/588 (40.65%), Postives = 326/588 (55.44%), Query Frame = 0

Query: 1   MASLSSLLLLSSYS---QTKTKSSISSNKRHVVV--NYVSCKAASNNGEDS--------- 60
           M + SSL+  +S+S     K++ S+S   +H V   + +SCKA +NN  ++         
Sbjct: 10  MGTYSSLISTNSFSTFLPNKSQLSLSGKSKHYVARRSSISCKATNNNNSNNQNEQQEESS 69

Query: 61  --VSKFDRREVLFGLGGLYGVVTATSS--VLAAPLT-PDIFNCHMAK-TPGGIK--DCCP 120
             + K DRR +L GLGGLYG  T        A P+  PD+  C  A+ TPGG +   CCP
Sbjct: 70  RLLGKLDRRNILIGLGGLYGATTLDRKPFAFADPIAPPDLTTCKPAEITPGGSETVPCCP 129

Query: 121 PQIGKIIHFVPPKIQQPYVRRPAAHLMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIH 180
           P   KI  F  P +  P    PAAH +    + + +     ++ L + DP S + QAK+H
Sbjct: 130 PVTTKIKTF-KPDLSIPLRTSPAAHQVTDEYLAKFKKAQAAMRALPDDDPRSMVQQAKVH 189

Query: 181 CAYCDYVYNQLGSITETRFGVHSNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDS 240
           CAYC+  Y Q+G  T+    VH + LFFPFHR YLYF+ERIL   I +P FA+P+WNWDS
Sbjct: 190 CAYCNGAYPQVG-FTDNDIQVHFSWLFFPFHRMYLYFYERILGKLIDDPTFALPYWNWDS 249

Query: 241 PPGMYMPEFYNRPSSSLYDHLRDPRHDPHKLIYLNYNGKTEDHSFDV-VDCNLRWMNDRM 300
           P G  +P+ Y   SS LYD  R+  H P  L+ L+Y GK +D      +D NL  M  +M
Sbjct: 250 PVGFPIPDIYTDTSSPLYDQYRNADHQPPVLVDLSYGGKDDDVDEQTRIDENLAIMYRQM 309

Query: 301 --QVQSPRSFLGRVLKAGETPNPTDMGTIEQSPHNNIHDWLGTKT-PHFEDMGSFYSAAL 360
               ++P  F G   +AG        GTIE  PHNNIH W+G  +  H EDMG+FYSA  
Sbjct: 310 VSGAKTPDLFFGHAYRAGNLNTGKYPGTIENMPHNNIHIWVGDPSQTHQEDMGNFYSAGR 369

Query: 361 DPLFYAHHANIDRLWNIWKTLDGNPKDHIDPDWLNSSFVFYDENKNAVNIKVSDCLDTEK 420
           DPLFYAHHAN+DR+WNIWKTL G  KD  D DWL++ F+FYDEN   V +KV D L+ EK
Sbjct: 370 DPLFYAHHANVDRMWNIWKTLGGKRKDITDTDWLDAEFLFYDENAELVRVKVRDSLEPEK 429

Query: 421 -LGYVYQEVPLPWLHMNKI--PTPNPWIRTEESEPPIVNFPQILNSDITTMVDRPEEDRS 480
            L Y Y+ V LPWL        T N       +      FP  L    T  V RP+  + 
Sbjct: 430 QLRYNYEPVSLPWLFTKPTARKTKNKTKAKVAATQLTSKFPATLVEVTTVEVARPKPRKR 489

Query: 481 G--EEVD--EVLVLEDVEYDPTTRVHFDVLVNVVDKAQDRCAAFLEFVGSFKNIPHG--L 540
              E+VD  E+L+++D+E++ T  V FDV +N   ++  R     EF GSF ++P G   
Sbjct: 490 SKKEKVDEEELLIIKDIEFEGTEAVKFDVFINDDAESLSR-RDKSEFAGSFVHVPQGKTT 549

Query: 541 HHHDKTSLRFVITEVIQGLGLERDKNLAVKIEPMVGGEGVTVGGIKIQ 554
               KT+L+  IT++++ LG E D ++ V + P V    +T+GG KI+
Sbjct: 550 KAKTKTNLKLGITDLLEDLGAEDDSSVLVTLVPRVSNSPITIGGFKIE 594

BLAST of CSPI01G11840 vs. ExPASy TrEMBL
Match: A0A0A0LUI1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G073090 PE=3 SV=1)

HSP 1 Score: 1168.7 bits (3022), Expect = 0.0e+00
Identity = 558/558 (100.00%), Postives = 558/558 (100.00%), Query Frame = 0

Query: 1   MASLSSLLLLSSYSQTKTKSSISSNKRHVVVNYVSCKAASNNGEDSVSKFDRREVLFGLG 60
           MASLSSLLLLSSYSQTKTKSSISSNKRHVVVNYVSCKAASNNGEDSVSKFDRREVLFGLG
Sbjct: 1   MASLSSLLLLSSYSQTKTKSSISSNKRHVVVNYVSCKAASNNGEDSVSKFDRREVLFGLG 60

Query: 61  GLYGVVTATSSVLAAPLTPDIFNCHMAKTPGGIKDCCPPQIGKIIHFVPPKIQQPYVRRP 120
           GLYGVVTATSSVLAAPLTPDIFNCHMAKTPGGIKDCCPPQIGKIIHFVPPKIQQPYVRRP
Sbjct: 61  GLYGVVTATSSVLAAPLTPDIFNCHMAKTPGGIKDCCPPQIGKIIHFVPPKIQQPYVRRP 120

Query: 121 AAHLMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIHCAYCDYVYNQLGSITETRFGVH 180
           AAHLMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIHCAYCDYVYNQLGSITETRFGVH
Sbjct: 121 AAHLMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIHCAYCDYVYNQLGSITETRFGVH 180

Query: 181 SNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMYMPEFYNRPSSSLYDHLR 240
           SNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMYMPEFYNRPSSSLYDHLR
Sbjct: 181 SNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMYMPEFYNRPSSSLYDHLR 240

Query: 241 DPRHDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQVQSPRSFLGRVLKAGETPNPTD 300
           DPRHDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQVQSPRSFLGRVLKAGETPNPTD
Sbjct: 241 DPRHDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQVQSPRSFLGRVLKAGETPNPTD 300

Query: 301 MGTIEQSPHNNIHDWLGTKTPHFEDMGSFYSAALDPLFYAHHANIDRLWNIWKTLDGNPK 360
           MGTIEQSPHNNIHDWLGTKTPHFEDMGSFYSAALDPLFYAHHANIDRLWNIWKTLDGNPK
Sbjct: 301 MGTIEQSPHNNIHDWLGTKTPHFEDMGSFYSAALDPLFYAHHANIDRLWNIWKTLDGNPK 360

Query: 361 DHIDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLPWLHMNKIPTPNPWIR 420
           DHIDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLPWLHMNKIPTPNPWIR
Sbjct: 361 DHIDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLPWLHMNKIPTPNPWIR 420

Query: 421 TEESEPPIVNFPQILNSDITTMVDRPEEDRSGEEVDEVLVLEDVEYDPTTRVHFDVLVNV 480
           TEESEPPIVNFPQILNSDITTMVDRPEEDRSGEEVDEVLVLEDVEYDPTTRVHFDVLVNV
Sbjct: 421 TEESEPPIVNFPQILNSDITTMVDRPEEDRSGEEVDEVLVLEDVEYDPTTRVHFDVLVNV 480

Query: 481 VDKAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLERDKNLAVKIEPMV 540
           VDKAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLERDKNLAVKIEPMV
Sbjct: 481 VDKAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLERDKNLAVKIEPMV 540

Query: 541 GGEGVTVGGIKIQLEPLR 559
           GGEGVTVGGIKIQLEPLR
Sbjct: 541 GGEGVTVGGIKIQLEPLR 558

BLAST of CSPI01G11840 vs. ExPASy TrEMBL
Match: A0A5A7UM88 (Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00520 PE=3 SV=1)

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 519/558 (93.01%), Postives = 537/558 (96.24%), Query Frame = 0

Query: 1   MASLSSLLLLSSYSQTKTKSSISSNKRHVVVNYVSCKAA-SNNGEDSVSKFDRREVLFGL 60
           MASLSSLLLLS YSQTKTK SISSNKRHVVVNYVSCKAA SNNGEDSVSKFDRREVLFGL
Sbjct: 1   MASLSSLLLLSPYSQTKTKFSISSNKRHVVVNYVSCKAASSNNGEDSVSKFDRREVLFGL 60

Query: 61  GGLYGVVTATSSVLAAPLTPDIFNCHMAKTPGGIKDCCPPQIGKIIHFVPPKIQQPYVRR 120
           GGLYGVVTAT SVLAAPLTPD+FNC MA+TPGGIK+CCPP+IGKIIHFVPPKIQQPYVRR
Sbjct: 61  GGLYGVVTATGSVLAAPLTPDVFNCDMAQTPGGIKNCCPPRIGKIIHFVPPKIQQPYVRR 120

Query: 121 PAAHLMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIHCAYCDYVYNQLGSITETRFGV 180
           PAAHLMNQTQIEELE GI+KLKQLDE DPHSF HQAKIHCAYCDY YNQLGSITETRF V
Sbjct: 121 PAAHLMNQTQIEELERGIQKLKQLDEDDPHSFSHQAKIHCAYCDYAYNQLGSITETRFEV 180

Query: 181 HSNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMYMPEFYNRPSSSLYDHL 240
           HSNALFFPFHRAYLYFFERILRHYIGNP+FAIPFWNWDSPPGM+MPEFYNRPSSSLYD+L
Sbjct: 181 HSNALFFPFHRAYLYFFERILRHYIGNPDFAIPFWNWDSPPGMHMPEFYNRPSSSLYDNL 240

Query: 241 RDPRHDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQVQSPRSFLGRVLKAGETPNPT 300
           RDPRHDPHKLI LNYNGKTEDHSFDVVDCNLRWMNDRM+VQSPRSFLGRVLKAGETP P+
Sbjct: 241 RDPRHDPHKLIDLNYNGKTEDHSFDVVDCNLRWMNDRMKVQSPRSFLGRVLKAGETPKPS 300

Query: 301 DMGTIEQSPHNNIHDWLGTKTPHFEDMGSFYSAALDPLFYAHHANIDRLWNIWKTLDGNP 360
           DMGTIEQSPHNNIHDW+GTKTPHFEDMGSFYSAALDPLFYAHH NIDRLWNIWKTLDGNP
Sbjct: 301 DMGTIEQSPHNNIHDWVGTKTPHFEDMGSFYSAALDPLFYAHHTNIDRLWNIWKTLDGNP 360

Query: 361 KDHIDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLPWLHMNKIPTPNPWI 420
           KDH DPDWLNSSFVFYDENKNAV I+VSDCLDTEKLGYVYQEVPLPWL +NKIP+PNPWI
Sbjct: 361 KDHTDPDWLNSSFVFYDENKNAVRIEVSDCLDTEKLGYVYQEVPLPWLLLNKIPSPNPWI 420

Query: 421 RTEESEPPIVNFPQILNSDITTMVDRPEEDRSGEEVDEVLVLEDVEYDPTTRVHFDVLVN 480
           RTEE EPPI+NFPQ LNSDITTMVDRPEE  SGEEVD VLVLEDVEYDPTTRVHFDVLVN
Sbjct: 421 RTEEPEPPIINFPQTLNSDITTMVDRPEEAGSGEEVDVVLVLEDVEYDPTTRVHFDVLVN 480

Query: 481 VVDKAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLERDKNLAVKIEPM 540
           VVD+AQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLERDKNLAVKIEPM
Sbjct: 481 VVDEAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLERDKNLAVKIEPM 540

Query: 541 VGGEGVTVGGIKIQLEPL 558
           VGGEGVT+GGIKIQLEP+
Sbjct: 541 VGGEGVTLGGIKIQLEPV 558

BLAST of CSPI01G11840 vs. ExPASy TrEMBL
Match: A0A1S3BFJ5 (polyphenol oxidase I, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103489300 PE=3 SV=1)

HSP 1 Score: 1030.4 bits (2663), Expect = 2.8e-297
Identity = 482/526 (91.63%), Postives = 504/526 (95.82%), Query Frame = 0

Query: 32  NYVSCKAASNNGEDSVSKFDRREVLFGLGGLYGVVTATSSVLAAPLTPDIFNCHMAKTPG 91
           ++ +   +SNNGEDSVSKFDRREVLFGLGGLYGVVTAT SVLAAPLTPD+FNC MA+TPG
Sbjct: 198 HHANVDPSSNNGEDSVSKFDRREVLFGLGGLYGVVTATGSVLAAPLTPDVFNCDMAQTPG 257

Query: 92  GIKDCCPPQIGKIIHFVPPKIQQPYVRRPAAHLMNQTQIEELESGIKKLKQLDEGDPHSF 151
           GIK+CCPP+IGKIIHFVPPKIQQPYVRRPAAHLMNQTQIEELE GI+KLKQLDE DPHSF
Sbjct: 258 GIKNCCPPRIGKIIHFVPPKIQQPYVRRPAAHLMNQTQIEELERGIQKLKQLDEDDPHSF 317

Query: 152 LHQAKIHCAYCDYVYNQLGSITETRFGVHSNALFFPFHRAYLYFFERILRHYIGNPNFAI 211
            HQAKIHCAYCDY YNQLGSITETRF VHSNALFFPFHRAYLYFFERILRHYIGNP+FAI
Sbjct: 318 SHQAKIHCAYCDYAYNQLGSITETRFEVHSNALFFPFHRAYLYFFERILRHYIGNPDFAI 377

Query: 212 PFWNWDSPPGMYMPEFYNRPSSSLYDHLRDPRHDPHKLIYLNYNGKTEDHSFDVVDCNLR 271
           PFWNWDSPPGM+MPEFYNRPSSSLYD+LRDPRHDPHKLI LNYNGKTEDHSFDVVDCNLR
Sbjct: 378 PFWNWDSPPGMHMPEFYNRPSSSLYDNLRDPRHDPHKLIDLNYNGKTEDHSFDVVDCNLR 437

Query: 272 WMNDRMQVQSPRSFLGRVLKAGETPNPTDMGTIEQSPHNNIHDWLGTKTPHFEDMGSFYS 331
           WMNDRM+VQSPRSFLGRVLKAGETP P+DMGTIEQSPHNNIHDW+GTKTPHFEDMGSFYS
Sbjct: 438 WMNDRMKVQSPRSFLGRVLKAGETPKPSDMGTIEQSPHNNIHDWVGTKTPHFEDMGSFYS 497

Query: 332 AALDPLFYAHHANIDRLWNIWKTLDGNPKDHIDPDWLNSSFVFYDENKNAVNIKVSDCLD 391
           AALDPLFYAHH NIDRLWNIWKTLDGNPKDH DPDWLNSSFVFYDENKNAV I+VSDCLD
Sbjct: 498 AALDPLFYAHHTNIDRLWNIWKTLDGNPKDHTDPDWLNSSFVFYDENKNAVRIEVSDCLD 557

Query: 392 TEKLGYVYQEVPLPWLHMNKIPTPNPWIRTEESEPPIVNFPQILNSDITTMVDRPEEDRS 451
           TEKLGYVYQEVPLPWL +NKIP+PNPWIRTEE EPPI+NFPQ LNSDITTMVDRPEE  S
Sbjct: 558 TEKLGYVYQEVPLPWLLLNKIPSPNPWIRTEEPEPPIINFPQTLNSDITTMVDRPEEAGS 617

Query: 452 GEEVDEVLVLEDVEYDPTTRVHFDVLVNVVDKAQDRCAAFLEFVGSFKNIPHGLHHHDKT 511
           GEEVD VLVLEDVEYDPTTRVHFDVLVNVVD+AQDRCAAFLEFVGSFKNIPHGLHHHDKT
Sbjct: 618 GEEVDVVLVLEDVEYDPTTRVHFDVLVNVVDEAQDRCAAFLEFVGSFKNIPHGLHHHDKT 677

Query: 512 SLRFVITEVIQGLGLERDKNLAVKIEPMVGGEGVTVGGIKIQLEPL 558
           SLRFVITEVIQGLGLERDKNLAVKIEPMVGGEGVT+GGIKIQLEP+
Sbjct: 678 SLRFVITEVIQGLGLERDKNLAVKIEPMVGGEGVTLGGIKIQLEPV 723

BLAST of CSPI01G11840 vs. ExPASy TrEMBL
Match: A0A5A7UTU0 (Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00530 PE=3 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 4.0e-179
Identity = 337/569 (59.23%), Postives = 403/569 (70.83%), Query Frame = 0

Query: 1   MASLSSLLLLSSYSQTKTKSS-ISSNKRHVVVNYVSCKAASNNGEDSVSKFDRREVLFGL 60
           MASLSSLL +S YS  K + S I  NKR  V N+VSCK ASNNGEDSV+KFDRR++LFGL
Sbjct: 1   MASLSSLLSVSPYSGCKIQPSVIPLNKRRGVNNHVSCK-ASNNGEDSVNKFDRRDMLFGL 60

Query: 61  GGLYGVVTATSSVLAA----PLTPDIFNCHMAK--TPGGIKDCCPPQIGKIIHFVPPKIQ 120
           GGLYGV T +   +A      L+PD+ NC   K    G   DCCPP+I +II F PP + 
Sbjct: 61  GGLYGVATLSGKDIALADDWSLSPDVHNCKPVKPSAVGKKMDCCPPKIKEIIEFTPPVVP 120

Query: 121 QPYVRRPAAHLMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIHCAYCDYVYNQLGSIT 180
            P V RPA HLM   QI +LE GI+ +K L E DP SF HQAK+HCAYCD  Y Q+ S  
Sbjct: 121 TPRV-RPAVHLMTPEQIADLEKGIQMMKMLPEDDPRSFAHQAKVHCAYCDDAYKQMDSTM 180

Query: 181 ETRFGVHSNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMYMPEFYNRPSS 240
           +  F VH NALFFPFHRAYLYFFERIL H +  P+FAIPFWNWD+PPGM MP+ Y +PSS
Sbjct: 181 D--FQVHENALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMCMPKIYTKPSS 240

Query: 241 SLYDHLRDPRHDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQVQSPRSFLGRVLKAG 300
           SLYD  RD  H P KL+ LNY GK E    ++VD NLRWMND+M V++ + FLG+V+K G
Sbjct: 241 SLYDENRDKCHQPPKLLNLNYGGK-EVKDPEIVDFNLRWMNDQMSVETAKEFLGKVIKRG 300

Query: 301 ETPNPTDMGTIEQSPHNNIHDWLG--TKTPHFEDMGSFYSAALDPLFYAHHANIDRLWNI 360
             P     G+IE SPHNNIH W+G    T H EDMG FYSA  DPLFYAHHAN+DR+WNI
Sbjct: 301 NGPE-NGSGSIETSPHNNIHIWVGDPEATIHGEDMGHFYSAGRDPLFYAHHANVDRMWNI 360

Query: 361 WKTLDGNPKDHIDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLPWLHMNK 420
           W+ +   P+DH D DWLNSSFV YDEN+ AV IKVS CLD++ LGYVYQ+VP+PW     
Sbjct: 361 WQKMKDRPRDHTDMDWLNSSFVLYDENRKAVRIKVSQCLDSKILGYVYQDVPIPWKDTPP 420

Query: 421 IPTPNPWIRTEESEPPIVNFPQILNSDITTMVDRPEEDRSGEEV--DEVLVLEDVEYDPT 480
           I  P    R+  + PP ++FPQIL+SDITTMVDRPE     E +  +EVLVLEDVEYDPT
Sbjct: 421 IRRPKQ-ERSLGNLPPTIDFPQILSSDITTMVDRPEVASPEERINKNEVLVLEDVEYDPT 480

Query: 481 TRVHFDVLVNVVDKAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLERD 540
             +HFDVL+NV D+ +D  A  +EFVG+F N+PHG   + KTSL FVIT+ IQ LGLE D
Sbjct: 481 LPLHFDVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRTTYHKTSLHFVITKKIQQLGLEGD 540

Query: 541 KNLAVKIEPMV-GGEGVTVGGIKIQLEPL 558
           K +AVKI P V GG+ + +GGIKIQLE +
Sbjct: 541 KKVAVKIVPNVNGGDRIKIGGIKIQLEDI 562

BLAST of CSPI01G11840 vs. ExPASy TrEMBL
Match: A0A5A7UM24 (Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G001410 PE=3 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 5.8e-178
Identity = 336/569 (59.05%), Postives = 404/569 (71.00%), Query Frame = 0

Query: 1   MASLSSLLLLSSYSQTKTKSS-ISSNKRHVVVNYVSCKAASNNGEDSVSKFDRREVLFGL 60
           MASLSSLL +S YS  K + S I  NKR  V N+VSCK ASNNGEDSV+KFDRR++LFGL
Sbjct: 1   MASLSSLLSVSPYSGCKIQPSVIPLNKRRGVNNHVSCK-ASNNGEDSVNKFDRRDMLFGL 60

Query: 61  GGLYGVVTATSSVLAA----PLTPDIFNCHMAK--TPGGIKDCCPPQIGKIIHFVPPKIQ 120
           GGLYGV T +   +A      L+PD+ NC   K    G   DCCPP+I +II F PP + 
Sbjct: 61  GGLYGVATLSGKDIALADDWSLSPDVHNCKPVKPSAVGKKMDCCPPKIKEIIEFTPPVVP 120

Query: 121 QPYVRRPAAHLMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIHCAYCDYVYNQLGSIT 180
            P V RPA HLM   QI +LE GI+ +K L E DP SF HQAK+HCAYCD  Y Q+ S  
Sbjct: 121 TPRV-RPAVHLMTPDQIADLEKGIQMMKMLPEDDPRSFAHQAKVHCAYCDDAYKQMDSTM 180

Query: 181 ETRFGVHSNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMYMPEFYNRPSS 240
           +  F VH NALFFPFHRAYLYFFERIL H +  P+FAIPFWNWD+PPGM MP+ Y +PSS
Sbjct: 181 D--FQVHENALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMCMPKIYTKPSS 240

Query: 241 SLYDHLRDPRHDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQVQSPRSFLGRVLKAG 300
           SLYD  RD  H P KL+ LNY GK    S ++VD NLRWMND+M V++ R FLG+V+K G
Sbjct: 241 SLYDENRDECHQPPKLLNLNYGGKKVKDS-EIVDFNLRWMNDQMSVETAREFLGKVIKRG 300

Query: 301 ETPNPTDMGTIEQSPHNNIHDWLG--TKTPHFEDMGSFYSAALDPLFYAHHANIDRLWNI 360
             P     G+IE SPHNN+H+W+G    T H EDMG FYSA  DPLFYAHHAN+DR+WNI
Sbjct: 301 NCPE-NGSGSIETSPHNNLHNWVGDPEATIHGEDMGHFYSAGRDPLFYAHHANVDRMWNI 360

Query: 361 WKTLDGNPKDHIDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLPWLHMNK 420
           W+ +  + +DH D DWLNSSFV YDEN+ AV IKVS CLD++ LGYVYQ+VP+PW     
Sbjct: 361 WQNMK-DRRDHTDMDWLNSSFVLYDENRKAVRIKVSQCLDSKILGYVYQDVPIPWKDTPP 420

Query: 421 IPTPNPWIRTEESEPPIVNFPQILNSDITTMVDRPEEDRSGEEV--DEVLVLEDVEYDPT 480
           I  P    R+  + PP ++FPQIL+SDITTMVDRPE     E +  +EVLVLEDVEYDPT
Sbjct: 421 IRRPKQ-ERSLGNLPPTIDFPQILSSDITTMVDRPEVASPEERINKNEVLVLEDVEYDPT 480

Query: 481 TRVHFDVLVNVVDKAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLERD 540
             +HFDVL+NV D+ +D  A  +EFVG+F N+PHG   + KTSL FVIT+ IQ LGLE D
Sbjct: 481 LPLHFDVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRTTYHKTSLHFVITKKIQQLGLEGD 540

Query: 541 KNLAVKIEPMV-GGEGVTVGGIKIQLEPL 558
           K +AVKI P V GG+ + +GGIKIQLE +
Sbjct: 541 KKVAVKIVPNVNGGDRIKIGGIKIQLEDI 561

BLAST of CSPI01G11840 vs. NCBI nr
Match: XP_004146641.1 (polyphenol oxidase, chloroplastic [Cucumis sativus] >KGN64649.1 hypothetical protein Csa_013698 [Cucumis sativus])

HSP 1 Score: 1168.7 bits (3022), Expect = 0.0e+00
Identity = 558/558 (100.00%), Postives = 558/558 (100.00%), Query Frame = 0

Query: 1   MASLSSLLLLSSYSQTKTKSSISSNKRHVVVNYVSCKAASNNGEDSVSKFDRREVLFGLG 60
           MASLSSLLLLSSYSQTKTKSSISSNKRHVVVNYVSCKAASNNGEDSVSKFDRREVLFGLG
Sbjct: 1   MASLSSLLLLSSYSQTKTKSSISSNKRHVVVNYVSCKAASNNGEDSVSKFDRREVLFGLG 60

Query: 61  GLYGVVTATSSVLAAPLTPDIFNCHMAKTPGGIKDCCPPQIGKIIHFVPPKIQQPYVRRP 120
           GLYGVVTATSSVLAAPLTPDIFNCHMAKTPGGIKDCCPPQIGKIIHFVPPKIQQPYVRRP
Sbjct: 61  GLYGVVTATSSVLAAPLTPDIFNCHMAKTPGGIKDCCPPQIGKIIHFVPPKIQQPYVRRP 120

Query: 121 AAHLMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIHCAYCDYVYNQLGSITETRFGVH 180
           AAHLMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIHCAYCDYVYNQLGSITETRFGVH
Sbjct: 121 AAHLMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIHCAYCDYVYNQLGSITETRFGVH 180

Query: 181 SNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMYMPEFYNRPSSSLYDHLR 240
           SNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMYMPEFYNRPSSSLYDHLR
Sbjct: 181 SNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMYMPEFYNRPSSSLYDHLR 240

Query: 241 DPRHDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQVQSPRSFLGRVLKAGETPNPTD 300
           DPRHDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQVQSPRSFLGRVLKAGETPNPTD
Sbjct: 241 DPRHDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQVQSPRSFLGRVLKAGETPNPTD 300

Query: 301 MGTIEQSPHNNIHDWLGTKTPHFEDMGSFYSAALDPLFYAHHANIDRLWNIWKTLDGNPK 360
           MGTIEQSPHNNIHDWLGTKTPHFEDMGSFYSAALDPLFYAHHANIDRLWNIWKTLDGNPK
Sbjct: 301 MGTIEQSPHNNIHDWLGTKTPHFEDMGSFYSAALDPLFYAHHANIDRLWNIWKTLDGNPK 360

Query: 361 DHIDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLPWLHMNKIPTPNPWIR 420
           DHIDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLPWLHMNKIPTPNPWIR
Sbjct: 361 DHIDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLPWLHMNKIPTPNPWIR 420

Query: 421 TEESEPPIVNFPQILNSDITTMVDRPEEDRSGEEVDEVLVLEDVEYDPTTRVHFDVLVNV 480
           TEESEPPIVNFPQILNSDITTMVDRPEEDRSGEEVDEVLVLEDVEYDPTTRVHFDVLVNV
Sbjct: 421 TEESEPPIVNFPQILNSDITTMVDRPEEDRSGEEVDEVLVLEDVEYDPTTRVHFDVLVNV 480

Query: 481 VDKAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLERDKNLAVKIEPMV 540
           VDKAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLERDKNLAVKIEPMV
Sbjct: 481 VDKAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLERDKNLAVKIEPMV 540

Query: 541 GGEGVTVGGIKIQLEPLR 559
           GGEGVTVGGIKIQLEPLR
Sbjct: 541 GGEGVTVGGIKIQLEPLR 558

BLAST of CSPI01G11840 vs. NCBI nr
Match: KAA0056962.1 (polyphenol oxidase I [Cucumis melo var. makuwa] >TYK26390.1 polyphenol oxidase I [Cucumis melo var. makuwa])

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 519/558 (93.01%), Postives = 537/558 (96.24%), Query Frame = 0

Query: 1   MASLSSLLLLSSYSQTKTKSSISSNKRHVVVNYVSCKAA-SNNGEDSVSKFDRREVLFGL 60
           MASLSSLLLLS YSQTKTK SISSNKRHVVVNYVSCKAA SNNGEDSVSKFDRREVLFGL
Sbjct: 1   MASLSSLLLLSPYSQTKTKFSISSNKRHVVVNYVSCKAASSNNGEDSVSKFDRREVLFGL 60

Query: 61  GGLYGVVTATSSVLAAPLTPDIFNCHMAKTPGGIKDCCPPQIGKIIHFVPPKIQQPYVRR 120
           GGLYGVVTAT SVLAAPLTPD+FNC MA+TPGGIK+CCPP+IGKIIHFVPPKIQQPYVRR
Sbjct: 61  GGLYGVVTATGSVLAAPLTPDVFNCDMAQTPGGIKNCCPPRIGKIIHFVPPKIQQPYVRR 120

Query: 121 PAAHLMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIHCAYCDYVYNQLGSITETRFGV 180
           PAAHLMNQTQIEELE GI+KLKQLDE DPHSF HQAKIHCAYCDY YNQLGSITETRF V
Sbjct: 121 PAAHLMNQTQIEELERGIQKLKQLDEDDPHSFSHQAKIHCAYCDYAYNQLGSITETRFEV 180

Query: 181 HSNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMYMPEFYNRPSSSLYDHL 240
           HSNALFFPFHRAYLYFFERILRHYIGNP+FAIPFWNWDSPPGM+MPEFYNRPSSSLYD+L
Sbjct: 181 HSNALFFPFHRAYLYFFERILRHYIGNPDFAIPFWNWDSPPGMHMPEFYNRPSSSLYDNL 240

Query: 241 RDPRHDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQVQSPRSFLGRVLKAGETPNPT 300
           RDPRHDPHKLI LNYNGKTEDHSFDVVDCNLRWMNDRM+VQSPRSFLGRVLKAGETP P+
Sbjct: 241 RDPRHDPHKLIDLNYNGKTEDHSFDVVDCNLRWMNDRMKVQSPRSFLGRVLKAGETPKPS 300

Query: 301 DMGTIEQSPHNNIHDWLGTKTPHFEDMGSFYSAALDPLFYAHHANIDRLWNIWKTLDGNP 360
           DMGTIEQSPHNNIHDW+GTKTPHFEDMGSFYSAALDPLFYAHH NIDRLWNIWKTLDGNP
Sbjct: 301 DMGTIEQSPHNNIHDWVGTKTPHFEDMGSFYSAALDPLFYAHHTNIDRLWNIWKTLDGNP 360

Query: 361 KDHIDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLPWLHMNKIPTPNPWI 420
           KDH DPDWLNSSFVFYDENKNAV I+VSDCLDTEKLGYVYQEVPLPWL +NKIP+PNPWI
Sbjct: 361 KDHTDPDWLNSSFVFYDENKNAVRIEVSDCLDTEKLGYVYQEVPLPWLLLNKIPSPNPWI 420

Query: 421 RTEESEPPIVNFPQILNSDITTMVDRPEEDRSGEEVDEVLVLEDVEYDPTTRVHFDVLVN 480
           RTEE EPPI+NFPQ LNSDITTMVDRPEE  SGEEVD VLVLEDVEYDPTTRVHFDVLVN
Sbjct: 421 RTEEPEPPIINFPQTLNSDITTMVDRPEEAGSGEEVDVVLVLEDVEYDPTTRVHFDVLVN 480

Query: 481 VVDKAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLERDKNLAVKIEPM 540
           VVD+AQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLERDKNLAVKIEPM
Sbjct: 481 VVDEAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLERDKNLAVKIEPM 540

Query: 541 VGGEGVTVGGIKIQLEPL 558
           VGGEGVT+GGIKIQLEP+
Sbjct: 541 VGGEGVTLGGIKIQLEPV 558

BLAST of CSPI01G11840 vs. NCBI nr
Match: XP_008446626.1 (PREDICTED: polyphenol oxidase I, chloroplastic-like [Cucumis melo])

HSP 1 Score: 1030.4 bits (2663), Expect = 5.7e-297
Identity = 482/526 (91.63%), Postives = 504/526 (95.82%), Query Frame = 0

Query: 32  NYVSCKAASNNGEDSVSKFDRREVLFGLGGLYGVVTATSSVLAAPLTPDIFNCHMAKTPG 91
           ++ +   +SNNGEDSVSKFDRREVLFGLGGLYGVVTAT SVLAAPLTPD+FNC MA+TPG
Sbjct: 198 HHANVDPSSNNGEDSVSKFDRREVLFGLGGLYGVVTATGSVLAAPLTPDVFNCDMAQTPG 257

Query: 92  GIKDCCPPQIGKIIHFVPPKIQQPYVRRPAAHLMNQTQIEELESGIKKLKQLDEGDPHSF 151
           GIK+CCPP+IGKIIHFVPPKIQQPYVRRPAAHLMNQTQIEELE GI+KLKQLDE DPHSF
Sbjct: 258 GIKNCCPPRIGKIIHFVPPKIQQPYVRRPAAHLMNQTQIEELERGIQKLKQLDEDDPHSF 317

Query: 152 LHQAKIHCAYCDYVYNQLGSITETRFGVHSNALFFPFHRAYLYFFERILRHYIGNPNFAI 211
            HQAKIHCAYCDY YNQLGSITETRF VHSNALFFPFHRAYLYFFERILRHYIGNP+FAI
Sbjct: 318 SHQAKIHCAYCDYAYNQLGSITETRFEVHSNALFFPFHRAYLYFFERILRHYIGNPDFAI 377

Query: 212 PFWNWDSPPGMYMPEFYNRPSSSLYDHLRDPRHDPHKLIYLNYNGKTEDHSFDVVDCNLR 271
           PFWNWDSPPGM+MPEFYNRPSSSLYD+LRDPRHDPHKLI LNYNGKTEDHSFDVVDCNLR
Sbjct: 378 PFWNWDSPPGMHMPEFYNRPSSSLYDNLRDPRHDPHKLIDLNYNGKTEDHSFDVVDCNLR 437

Query: 272 WMNDRMQVQSPRSFLGRVLKAGETPNPTDMGTIEQSPHNNIHDWLGTKTPHFEDMGSFYS 331
           WMNDRM+VQSPRSFLGRVLKAGETP P+DMGTIEQSPHNNIHDW+GTKTPHFEDMGSFYS
Sbjct: 438 WMNDRMKVQSPRSFLGRVLKAGETPKPSDMGTIEQSPHNNIHDWVGTKTPHFEDMGSFYS 497

Query: 332 AALDPLFYAHHANIDRLWNIWKTLDGNPKDHIDPDWLNSSFVFYDENKNAVNIKVSDCLD 391
           AALDPLFYAHH NIDRLWNIWKTLDGNPKDH DPDWLNSSFVFYDENKNAV I+VSDCLD
Sbjct: 498 AALDPLFYAHHTNIDRLWNIWKTLDGNPKDHTDPDWLNSSFVFYDENKNAVRIEVSDCLD 557

Query: 392 TEKLGYVYQEVPLPWLHMNKIPTPNPWIRTEESEPPIVNFPQILNSDITTMVDRPEEDRS 451
           TEKLGYVYQEVPLPWL +NKIP+PNPWIRTEE EPPI+NFPQ LNSDITTMVDRPEE  S
Sbjct: 558 TEKLGYVYQEVPLPWLLLNKIPSPNPWIRTEEPEPPIINFPQTLNSDITTMVDRPEEAGS 617

Query: 452 GEEVDEVLVLEDVEYDPTTRVHFDVLVNVVDKAQDRCAAFLEFVGSFKNIPHGLHHHDKT 511
           GEEVD VLVLEDVEYDPTTRVHFDVLVNVVD+AQDRCAAFLEFVGSFKNIPHGLHHHDKT
Sbjct: 618 GEEVDVVLVLEDVEYDPTTRVHFDVLVNVVDEAQDRCAAFLEFVGSFKNIPHGLHHHDKT 677

Query: 512 SLRFVITEVIQGLGLERDKNLAVKIEPMVGGEGVTVGGIKIQLEPL 558
           SLRFVITEVIQGLGLERDKNLAVKIEPMVGGEGVT+GGIKIQLEP+
Sbjct: 678 SLRFVITEVIQGLGLERDKNLAVKIEPMVGGEGVTLGGIKIQLEPV 723

BLAST of CSPI01G11840 vs. NCBI nr
Match: KAA0056965.1 (polyphenol oxidase I [Cucumis melo var. makuwa] >TYK26391.1 polyphenol oxidase I [Cucumis melo var. makuwa] >TYK26393.1 polyphenol oxidase I [Cucumis melo var. makuwa])

HSP 1 Score: 637.9 bits (1644), Expect = 8.3e-179
Identity = 337/569 (59.23%), Postives = 403/569 (70.83%), Query Frame = 0

Query: 1   MASLSSLLLLSSYSQTKTKSS-ISSNKRHVVVNYVSCKAASNNGEDSVSKFDRREVLFGL 60
           MASLSSLL +S YS  K + S I  NKR  V N+VSCK ASNNGEDSV+KFDRR++LFGL
Sbjct: 1   MASLSSLLSVSPYSGCKIQPSVIPLNKRRGVNNHVSCK-ASNNGEDSVNKFDRRDMLFGL 60

Query: 61  GGLYGVVTATSSVLAA----PLTPDIFNCHMAK--TPGGIKDCCPPQIGKIIHFVPPKIQ 120
           GGLYGV T +   +A      L+PD+ NC   K    G   DCCPP+I +II F PP + 
Sbjct: 61  GGLYGVATLSGKDIALADDWSLSPDVHNCKPVKPSAVGKKMDCCPPKIKEIIEFTPPVVP 120

Query: 121 QPYVRRPAAHLMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIHCAYCDYVYNQLGSIT 180
            P V RPA HLM   QI +LE GI+ +K L E DP SF HQAK+HCAYCD  Y Q+ S  
Sbjct: 121 TPRV-RPAVHLMTPEQIADLEKGIQMMKMLPEDDPRSFAHQAKVHCAYCDDAYKQMDSTM 180

Query: 181 ETRFGVHSNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMYMPEFYNRPSS 240
           +  F VH NALFFPFHRAYLYFFERIL H +  P+FAIPFWNWD+PPGM MP+ Y +PSS
Sbjct: 181 D--FQVHENALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMCMPKIYTKPSS 240

Query: 241 SLYDHLRDPRHDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQVQSPRSFLGRVLKAG 300
           SLYD  RD  H P KL+ LNY GK E    ++VD NLRWMND+M V++ + FLG+V+K G
Sbjct: 241 SLYDENRDKCHQPPKLLNLNYGGK-EVKDPEIVDFNLRWMNDQMSVETAKEFLGKVIKRG 300

Query: 301 ETPNPTDMGTIEQSPHNNIHDWLG--TKTPHFEDMGSFYSAALDPLFYAHHANIDRLWNI 360
             P     G+IE SPHNNIH W+G    T H EDMG FYSA  DPLFYAHHAN+DR+WNI
Sbjct: 301 NGPE-NGSGSIETSPHNNIHIWVGDPEATIHGEDMGHFYSAGRDPLFYAHHANVDRMWNI 360

Query: 361 WKTLDGNPKDHIDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLPWLHMNK 420
           W+ +   P+DH D DWLNSSFV YDEN+ AV IKVS CLD++ LGYVYQ+VP+PW     
Sbjct: 361 WQKMKDRPRDHTDMDWLNSSFVLYDENRKAVRIKVSQCLDSKILGYVYQDVPIPWKDTPP 420

Query: 421 IPTPNPWIRTEESEPPIVNFPQILNSDITTMVDRPEEDRSGEEV--DEVLVLEDVEYDPT 480
           I  P    R+  + PP ++FPQIL+SDITTMVDRPE     E +  +EVLVLEDVEYDPT
Sbjct: 421 IRRPKQ-ERSLGNLPPTIDFPQILSSDITTMVDRPEVASPEERINKNEVLVLEDVEYDPT 480

Query: 481 TRVHFDVLVNVVDKAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLERD 540
             +HFDVL+NV D+ +D  A  +EFVG+F N+PHG   + KTSL FVIT+ IQ LGLE D
Sbjct: 481 LPLHFDVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRTTYHKTSLHFVITKKIQQLGLEGD 540

Query: 541 KNLAVKIEPMV-GGEGVTVGGIKIQLEPL 558
           K +AVKI P V GG+ + +GGIKIQLE +
Sbjct: 541 KKVAVKIVPNVNGGDRIKIGGIKIQLEDI 562

BLAST of CSPI01G11840 vs. NCBI nr
Match: KAA0056963.1 (polyphenol oxidase I [Cucumis melo var. makuwa])

HSP 1 Score: 634.0 bits (1634), Expect = 1.2e-177
Identity = 336/569 (59.05%), Postives = 404/569 (71.00%), Query Frame = 0

Query: 1   MASLSSLLLLSSYSQTKTKSS-ISSNKRHVVVNYVSCKAASNNGEDSVSKFDRREVLFGL 60
           MASLSSLL +S YS  K + S I  NKR  V N+VSCK ASNNGEDSV+KFDRR++LFGL
Sbjct: 1   MASLSSLLSVSPYSGCKIQPSVIPLNKRRGVNNHVSCK-ASNNGEDSVNKFDRRDMLFGL 60

Query: 61  GGLYGVVTATSSVLAA----PLTPDIFNCHMAK--TPGGIKDCCPPQIGKIIHFVPPKIQ 120
           GGLYGV T +   +A      L+PD+ NC   K    G   DCCPP+I +II F PP + 
Sbjct: 61  GGLYGVATLSGKDIALADDWSLSPDVHNCKPVKPSAVGKKMDCCPPKIKEIIEFTPPVVP 120

Query: 121 QPYVRRPAAHLMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIHCAYCDYVYNQLGSIT 180
            P V RPA HLM   QI +LE GI+ +K L E DP SF HQAK+HCAYCD  Y Q+ S  
Sbjct: 121 TPRV-RPAVHLMTPDQIADLEKGIQMMKMLPEDDPRSFAHQAKVHCAYCDDAYKQMDSTM 180

Query: 181 ETRFGVHSNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMYMPEFYNRPSS 240
           +  F VH NALFFPFHRAYLYFFERIL H +  P+FAIPFWNWD+PPGM MP+ Y +PSS
Sbjct: 181 D--FQVHENALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMCMPKIYTKPSS 240

Query: 241 SLYDHLRDPRHDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQVQSPRSFLGRVLKAG 300
           SLYD  RD  H P KL+ LNY GK    S ++VD NLRWMND+M V++ R FLG+V+K G
Sbjct: 241 SLYDENRDECHQPPKLLNLNYGGKKVKDS-EIVDFNLRWMNDQMSVETAREFLGKVIKRG 300

Query: 301 ETPNPTDMGTIEQSPHNNIHDWLG--TKTPHFEDMGSFYSAALDPLFYAHHANIDRLWNI 360
             P     G+IE SPHNN+H+W+G    T H EDMG FYSA  DPLFYAHHAN+DR+WNI
Sbjct: 301 NCPE-NGSGSIETSPHNNLHNWVGDPEATIHGEDMGHFYSAGRDPLFYAHHANVDRMWNI 360

Query: 361 WKTLDGNPKDHIDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLPWLHMNK 420
           W+ +  + +DH D DWLNSSFV YDEN+ AV IKVS CLD++ LGYVYQ+VP+PW     
Sbjct: 361 WQNMK-DRRDHTDMDWLNSSFVLYDENRKAVRIKVSQCLDSKILGYVYQDVPIPWKDTPP 420

Query: 421 IPTPNPWIRTEESEPPIVNFPQILNSDITTMVDRPEEDRSGEEV--DEVLVLEDVEYDPT 480
           I  P    R+  + PP ++FPQIL+SDITTMVDRPE     E +  +EVLVLEDVEYDPT
Sbjct: 421 IRRPKQ-ERSLGNLPPTIDFPQILSSDITTMVDRPEVASPEERINKNEVLVLEDVEYDPT 480

Query: 481 TRVHFDVLVNVVDKAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLERD 540
             +HFDVL+NV D+ +D  A  +EFVG+F N+PHG   + KTSL FVIT+ IQ LGLE D
Sbjct: 481 LPLHFDVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRTTYHKTSLHFVITKKIQQLGLEGD 540

Query: 541 KNLAVKIEPMV-GGEGVTVGGIKIQLEPL 558
           K +AVKI P V GG+ + +GGIKIQLE +
Sbjct: 541 KKVAVKIVPNVNGGDRIKIGGIKIQLEDI 561

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P433116.1e-11641.62Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1[more]
P433091.4e-11541.18Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1[more]
Q6UIL35.3e-11241.91(+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 S... [more]
Q062153.2e-10939.31Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1[more]
O811031.2e-10840.65Polyphenol oxidase latent form, chloroplastic OS=Prunus armeniaca OX=36596 GN=PP... [more]
Match NameE-valueIdentityDescription
A0A0A0LUI10.0e+00100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G073090 PE=3 SV=1[more]
A0A5A7UM880.0e+0093.01Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861... [more]
A0A1S3BFJ52.8e-29791.63polyphenol oxidase I, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103489300... [more]
A0A5A7UTU04.0e-17959.23Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861... [more]
A0A5A7UM245.8e-17859.05Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G... [more]
Match NameE-valueIdentityDescription
XP_004146641.10.0e+00100.00polyphenol oxidase, chloroplastic [Cucumis sativus] >KGN64649.1 hypothetical pro... [more]
KAA0056962.10.0e+0093.01polyphenol oxidase I [Cucumis melo var. makuwa] >TYK26390.1 polyphenol oxidase I... [more]
XP_008446626.15.7e-29791.63PREDICTED: polyphenol oxidase I, chloroplastic-like [Cucumis melo][more]
KAA0056965.18.3e-17959.23polyphenol oxidase I [Cucumis melo var. makuwa] >TYK26391.1 polyphenol oxidase I... [more]
KAA0056963.11.2e-17759.05polyphenol oxidase I [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002227Tyrosinase copper-binding domainPRINTSPR00092TYROSINASEcoord: 308..319
score: 38.95
coord: 334..352
score: 53.92
coord: 211..216
score: 57.82
coord: 180..197
score: 52.04
IPR002227Tyrosinase copper-binding domainPFAMPF00264Tyrosinasecoord: 149..354
e-value: 2.7E-31
score: 109.6
IPR002227Tyrosinase copper-binding domainPROSITEPS00498TYROSINASE_2coord: 335..346
IPR002227Tyrosinase copper-binding domainPROSITEPS00497TYROSINASE_1coord: 180..197
IPR022740Polyphenol oxidase, C-terminalPFAMPF12143PPO1_KFDVcoord: 431..554
e-value: 9.5E-28
score: 96.9
IPR022739Polyphenol oxidase, central domainPFAMPF12142PPO1_DWLcoord: 361..408
e-value: 2.3E-24
score: 84.9
IPR008922Uncharacterised domain, di-copper centreGENE3D1.10.1280.10coord: 77..408
e-value: 9.0E-100
score: 336.4
IPR008922Uncharacterised domain, di-copper centreSUPERFAMILY48056Di-copper centre-containing domaincoord: 79..407
NoneNo IPR availablePANTHERPTHR11474TYROSINASE FAMILY MEMBERcoord: 35..554
NoneNo IPR availablePANTHERPTHR11474:SF115OS04G0624500 PROTEINcoord: 35..554

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G11840.1CSPI01G11840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004097 catechol oxidase activity
molecular_function GO:0016491 oxidoreductase activity