CSPI01G09010 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G09010
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Pectinesterase
Location	Chr1: 5641824 .. 5643181 (+)
RNA-Seq Expression	CSPI01G09010
Synteny	CSPI01G09010

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTTCATGTCCTCTTCATCTCATTTTCTAATGTAATTTTGCATTAGCTAGCAAGCTTTTCCCTCTTTGCATTTACTTGTTTCTTTTTTCTTCTAATTCAACTAGGAAAATGCTATTTTTCTTTGGTGTTCCATGTTCTCTCTTCTGCTTCTTCAATCTCATGCTACTCATTCATTCCCTAAGTTTTAATCTAACAGTATCGTTAGATGGATCGGGCAATTTCAAACGCATTAGCGATGCAATAGCAGCCGCTCCTAACAATTCCAATACCCGATTTTATATTCATGTAGCCCCTGGCACTTACCATGAAACTCTCCAAATTCCAAGGCTTAAGAAGTTCATTGCTTTGGTAGGCGATGATCCATTCACGACCATTATTATAGACAATCGAAGTAATGGAACCGGATTCAAGACCAATAACTCTGCAACCCTTAGTAACCTTCTAACTTCTACATCTTTCTTTAAATAATGGTCTCTGTACATTGATATTCACACCAAACTGTTGAATTGAGGTTATGAAATTTCGGCCACAATTATGTTTTAATTTTGAAGTAAGATAAATAGTATGACTAAGGGTTTTTTAAGGTACAAACTTGGACAAATGTATTGGTTTAAATAATTATTATATACATTATTGAATTTTGTTTTCTGATCTTATTTTTATTTGATGAAGCCGTCCAAGGTGATAACTTTATGGCAGAATCTTTAACGTTTGAAAACTCAGCCGGACCTCAAAACAATCAGGCAGTAGCGGTCTTTGACAAAGCCAACCATACTGCTTACTATAAGTGCAGATTTTTGAGTTTTCAAGACACGTTATATGTCAACGGAAAGCCCCAATTCTTTAAGGAGTCCGACATATATGGAAGTGTTGATTTCATCTGTGGATATGGGCAAGTAATGTTTCAAGATTGCAATATATATGCTCGGATGCCGATCAATTCTATCACAGTCACAGCACAATCCAAATATATTTTACGTTCTGTCAGCGGTTTTTCATTTCAAAACTGTACAGTCACAGTTTCACGTGAAATATCTTCAAATAAACAGAACGTTAAAGTGTTTCTCGGTCGTCCATGGAAACAATATTCAAAAGTTGTGTTTATGGAATCATTTCTCGATGATGTGGTGGCGTCGGAAGGGTGGGTGGAGTGGATAGGGGTACCAGTTAACAACTTGTTCTATGGAGAGTTCAACAACTGCGGTCCTGGGGCTGACGTTTCCAAAAGAGTTAATTGGACTAGCTATCATTTATTAGATAAGGAATCAGCCCTTCGTTTTACGGTAGATAACTTTGTAAATGGATCTGAATGGTTGCCTGAAACTGGAATTCCCTTTAGACGTGGACTTCTTTCCTAA

mRNA sequence

GTTTCATGTCCTCTTCATCTCATTTTCTAATGTAATTTTGCATTAGCTAGCAAGCTTTTCCCTCTTTGCATTTACTTGTTTCTTTTTTCTTCTAATTCAACTAGGAAAATGCTATTTTTCTTTGGTGTTCCATGTTCTCTCTTCTGCTTCTTCAATCTCATGCTACTCATTCATTCCCTAAGTTTTAATCTAACAGTATCGTTAGATGGATCGGGCAATTTCAAACGCATTAGCGATGCAATAGCAGCCGCTCCTAACAATTCCAATACCCGATTTTATATTCATGTAGCCCCTGGCACTTACCATGAAACTCTCCAAATTCCAAGGCTTAAGAAGTTCATTGCTTTGGTAGGCGATGATCCATTCACGACCATTATTATAGACAATCGAAGTAATGGAACCGGATTCAAGACCAATAACTCTGCAACCCTTACCGTCCAAGGTGATAACTTTATGGCAGAATCTTTAACGTTTGAAAACTCAGCCGGACCTCAAAACAATCAGGCAGTAGCGGTCTTTGACAAAGCCAACCATACTGCTTACTATAAGTGCAGATTTTTGAGTTTTCAAGACACGTTATATGTCAACGGAAAGCCCCAATTCTTTAAGGAGTCCGACATATATGGAAGTGTTGATTTCATCTGTGGATATGGGCAAGTAATGTTTCAAGATTGCAATATATATGCTCGGATGCCGATCAATTCTATCACAGTCACAGCACAATCCAAATATATTTTACGTTCTGTCAGCGGTTTTTCATTTCAAAACTGTACAGTCACAGTTTCACGTGAAATATCTTCAAATAAACAGAACGTTAAAGTGTTTCTCGGTCGTCCATGGAAACAATATTCAAAAGTTGTGTTTATGGAATCATTTCTCGATGATGTGGTGGCGTCGGAAGGGTGGGTGGAGTGGATAGGGGTACCAGTTAACAACTTGTTCTATGGAGAGTTCAACAACTGCGGTCCTGGGGCTGACGTTTCCAAAAGAGTTAATTGGACTAGCTATCATTTATTAGATAAGGAATCAGCCCTTCGTTTTACGGTAGATAACTTTGTAAATGGATCTGAATGGTTGCCTGAAACTGGAATTCCCTTTAGACGTGGACTTCTTTCCTAA

Coding sequence (CDS)

ATGCTATTTTTCTTTGGTGTTCCATGTTCTCTCTTCTGCTTCTTCAATCTCATGCTACTCATTCATTCCCTAAGTTTTAATCTAACAGTATCGTTAGATGGATCGGGCAATTTCAAACGCATTAGCGATGCAATAGCAGCCGCTCCTAACAATTCCAATACCCGATTTTATATTCATGTAGCCCCTGGCACTTACCATGAAACTCTCCAAATTCCAAGGCTTAAGAAGTTCATTGCTTTGGTAGGCGATGATCCATTCACGACCATTATTATAGACAATCGAAGTAATGGAACCGGATTCAAGACCAATAACTCTGCAACCCTTACCGTCCAAGGTGATAACTTTATGGCAGAATCTTTAACGTTTGAAAACTCAGCCGGACCTCAAAACAATCAGGCAGTAGCGGTCTTTGACAAAGCCAACCATACTGCTTACTATAAGTGCAGATTTTTGAGTTTTCAAGACACGTTATATGTCAACGGAAAGCCCCAATTCTTTAAGGAGTCCGACATATATGGAAGTGTTGATTTCATCTGTGGATATGGGCAAGTAATGTTTCAAGATTGCAATATATATGCTCGGATGCCGATCAATTCTATCACAGTCACAGCACAATCCAAATATATTTTACGTTCTGTCAGCGGTTTTTCATTTCAAAACTGTACAGTCACAGTTTCACGTGAAATATCTTCAAATAAACAGAACGTTAAAGTGTTTCTCGGTCGTCCATGGAAACAATATTCAAAAGTTGTGTTTATGGAATCATTTCTCGATGATGTGGTGGCGTCGGAAGGGTGGGTGGAGTGGATAGGGGTACCAGTTAACAACTTGTTCTATGGAGAGTTCAACAACTGCGGTCCTGGGGCTGACGTTTCCAAAAGAGTTAATTGGACTAGCTATCATTTATTAGATAAGGAATCAGCCCTTCGTTTTACGGTAGATAACTTTGTAAATGGATCTGAATGGTTGCCTGAAACTGGAATTCCCTTTAGACGTGGACTTCTTTCCTAA

Protein sequence

MLFFFGVPCSLFCFFNLMLLIHSLSFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHVAPGTYHETLQIPRLKKFIALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQAVAVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIYARMPINSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVFMESFLDDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTSYHLLDKESALRFTVDNFVNGSEWLPETGIPFRRGLLS*

Homology

[Close]

BLAST of CSPI01G09010 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 1.1e-75
Identity = 140/311 (45.02%), Postives = 203/311 (65.27%), Query Frame = 0


Query: 27  NLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHVAPGTYHETLQIPRLKKFIALVGDDPF 86
           N+ V+ DGSGN K I +A+AAA     +R+ I++  GTY+E +++ +LK  I  VGD   
Sbjct: 202 NIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTYNENIEV-KLKN-IMFVGDGIG 261

Query: 87  TTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQAVAVFDKANHTAYY 146
            TII  ++S G G  T  SAT+ V GDNF+A  +T  N+AGP N+QAVA+   ++ + +Y
Sbjct: 262 KTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFY 321

Query: 147 KCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIYARMPIN-SITVTAQ 206
           +C F  +QDTLYV+ + QF++E DIYG+VDFI G   V+ Q+CNI+AR P N + T+TAQ
Sbjct: 322 RCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPNRTNTLTAQ 381

Query: 207 SKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVFMESFLDDVVASEG 266
            +      +G    NC VT + ++   + +VK FLGRPWKQYS+ V++++FLD ++   G
Sbjct: 382 GRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAG 441

Query: 267 WVEWIG-VPVNNLFYGEFNNCGPGADVSKRVNWTSYHLLDKESAL-RFTVDNFVNGSEWL 326
           W+EW G   +N L+Y E+ N GPG+  + RV W  YH+L   S + +FTV NF+ G+ WL
Sbjct: 442 WMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWL 501

Query: 327 PETGIPFRRGL 335
           P T +PF  GL
Sbjct: 502 PATNVPFTSGL 510

[Close]

BLAST of CSPI01G09010 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 3.5e-74
Identity = 136/308 (44.16%), Postives = 197/308 (63.96%), Query Frame = 0


Query: 35  SGNFKRISDAIAAAPNNSNTR---FYIHVAPGTYHETLQIPRLKKFIALVGDDPFTTIII 94
           S NF  I+DAIAAAPNN+      F I+   G Y E + +P  KK + L+GD    TII 
Sbjct: 310 SDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIIT 369

Query: 95  DNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQAVAVFDKANHTAYYKCRFL 154
            N +   G+ T N ++  V G+ FMA  +TF N+AGP+ +QAVA+ + A  +++Y+C F 
Sbjct: 370 GNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFE 429

Query: 155 SFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIYARMPI--NSITVTAQSKYI 214
            +QDTLYV+   QF++E DIYG+VDFI G    +FQ+CNIYAR P+      +TA  +  
Sbjct: 430 GYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLD 489

Query: 215 LRSVSGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVFMESFLDDVVASEGWVEW 274
               +G S  NCT+  + ++++  ++   FLGRPWK YS+ VFM+S++ D+V   GW+EW
Sbjct: 490 PNQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEW 549

Query: 275 IG-VPVNNLFYGEFNNCGPGADVSKRVNWTSYHLLDKESALRFTVDNFVNGSEWLPETGI 334
            G + ++ ++YGE++N GPGA+ ++RV W  Y+LL+   A+ FTV NF  G  WLP+T I
Sbjct: 550 NGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQTDI 609

Query: 335 PFRRGLLS 337
           PF  GLLS
Sbjct: 610 PFYGGLLS 617

[Close]

BLAST of CSPI01G09010 vs. ExPASy Swiss-Prot
Match: O48711 (Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PME12 PE=2 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 4.5e-74
Identity = 132/313 (42.17%), Postives = 194/313 (61.98%), Query Frame = 0


Query: 25  SFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHVAPGTYHETLQIPRLKKFIALVGDD 84
           S +L V+ DG+GNF  I++AI+ APN SN R  I+V  G Y E + IP  K  I L+GD 
Sbjct: 235 SESLVVAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDG 294

Query: 85  PFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQAVAVFDKANHTA 144
              T I  NRS G G+ T  SATL V G+ F+A  +   N+AGP+ +QAVA+   A+  A
Sbjct: 295 SDVTFITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVA 354

Query: 145 YYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIYARMPI--NSITV 204
            Y+C    +QDTLY +   QF++E DIYG++D+I G   V+FQ CNI +++P+      +
Sbjct: 355 LYRCVIDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVI 414

Query: 205 TAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVFMESFLDDVVA 264
           TAQS+      +G S QNC++  S ++ ++   VK +LGRPW+++S+ V MES++D+ + 
Sbjct: 415 TAQSRDTQDEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMESYIDEFID 474

Query: 265 SEGWVEWI-GVPVNNLFYGEFNNCGPGADVSKRVNWTSYHLLDKESALRFTVDNFVNGSE 324
             GW +W  G  ++ L+YGE+NN GPG++  KRVNW  +H++  E A  FT   F+ G  
Sbjct: 475 GSGWSKWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIMGYEDAFNFTATEFITGDG 534

Query: 325 WLPETGIPFRRGL 335
           WL  T  P+  G+
Sbjct: 535 WLGSTSFPYDNGI 547

[Close]

BLAST of CSPI01G09010 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 5.9e-74
Identity = 136/325 (41.85%), Postives = 207/325 (63.69%), Query Frame = 0


Query: 18  MLLIHSLSFNLTVSLDGSGNFKRISDAIAAAPNNSNTR---FYIHVAPGTYHETLQIPRL 77
           M+LI  +   +TVS DG+GNF  I+ A+AAAPNN++     F I+V  G Y E + I + 
Sbjct: 258 MVLISDI---VTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKN 317

Query: 78  KKFIALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQAV 137
           K+++ ++GD    T++  NRS   G+ T NSAT  V   NF+A ++TF N+AGP+ +QAV
Sbjct: 318 KRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAV 377

Query: 138 AVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIYAR 197
           A+   A+ + +Y C F ++QDTLY +   QF++E D+YG+VDFI G   V+FQ+CN+Y R
Sbjct: 378 ALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPR 437

Query: 198 --MPINSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVF 257
             MP     +TAQ +      +G S QNCT+  + ++ S+   VK +LGRPWK+YS+ V+
Sbjct: 438 KPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVY 497

Query: 258 MESFLDDVVASEGWVEWIG-VPVNNLFYGEFNNCGPGADVSKRVNWTSYHLLDKESALRF 317
           M+S++D  V   GW EW G   ++ L+Y E+NN GPG++ + RV W  YH+++   A  F
Sbjct: 498 MQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAANF 557

Query: 318 TVDNFVNGSEWLPETGIPFRRGLLS 337
           TV      ++W+ +TG+P+  GL+S
Sbjct: 558 TVTGLFIEADWIWKTGVPYTSGLIS 579

[Close]

BLAST of CSPI01G09010 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 278.9 bits (712), Expect = 7.7e-74
Identity = 131/315 (41.59%), Postives = 204/315 (64.76%), Query Frame = 0


Query: 28  LTVSLDGSGNFKRISDAIAAAPNNSNTR---FYIHVAPGTYHETLQIPRLKKFIALVGDD 87
           +TV+ +G+GNF  I++A+ +APN ++     F I+V  G Y E + I + K+++ ++GD 
Sbjct: 259 VTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKRYLMMIGDG 318

Query: 88  PFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQAVAVFDKANHTA 147
              T++  NR+   G+ T NSAT  V   NF+A ++TF N+AGP+ +QAVA+   A+ + 
Sbjct: 319 INRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAMRSSADLSI 378

Query: 148 YYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIYARMPINS--ITV 207
           +Y C F ++QDTLY +   QF++E DIYG+VDFI G   V+FQDCN+Y R P+ +    +
Sbjct: 379 FYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPMQNQFNAI 438

Query: 208 TAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVFMESFLDDVVA 267
           TAQ +      +G S  NCT+  + ++ S+   VK +LGRPWK+YS+ VFM+S++D+VV 
Sbjct: 439 TAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVE 498

Query: 268 SEGWVEWIG-VPVNNLFYGEFNNCGPGADVSKRVNWTSYHLLDKESALRFTVDNFVNGSE 327
             GW EW G   ++ L+Y E+NN G G+  + RV W  YH+++   A  FTV+NF+ G  
Sbjct: 499 PVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFTVENFLLGDG 558

Query: 328 WLPETGIPFRRGLLS 337
           W+ ++G+P+  GLLS
Sbjct: 559 WMVQSGVPYISGLLS 573

[Close]

BLAST of CSPI01G09010 vs. ExPASy TrEMBL
Match: A0A0A0LR14 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G049450 PE=4 SV=1)

HSP 1 Score: 688.0 bits (1774), Expect = 2.0e-194
Identity = 335/336 (99.70%), Postives = 335/336 (99.70%), Query Frame = 0


Query: 1   MLFFFGVPCSLFCFFNLMLLIHSLSFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHV 60
           MLFFFGVPCSLFCFFNLMLLIHSLSFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHV
Sbjct: 1   MLFFFGVPCSLFCFFNLMLLIHSLSFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHV 60

Query: 61  APGTYHETLQIPRLKKFIALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESL 120
           APGTYHETLQIPRLKKFIALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESL
Sbjct: 61  APGTYHETLQIPRLKKFIALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESL 120

Query: 121 TFENSAGPQNNQAVAVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICG 180
           TFENSAGPQNNQAVAVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICG
Sbjct: 121 TFENSAGPQNNQAVAVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICG 180

Query: 181 YGQVMFQDCNIYARMPINSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFL 240
           YGQVMFQDCNIYARMPINSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFL
Sbjct: 181 YGQVMFQDCNIYARMPINSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFL 240

Query: 241 GRPWKQYSKVVFMESFLDDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTSY 300
           GRPWKQYSKVVFMESFLDDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTSY
Sbjct: 241 GRPWKQYSKVVFMESFLDDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTSY 300

Query: 301 HLLDKESALRFTVDNFVNGSEWLPETGIPFRRGLLS 337
           HLLDKESALRFTVDNFVNGSEWLPETGIPFRRGL S
Sbjct: 301 HLLDKESALRFTVDNFVNGSEWLPETGIPFRRGLHS 336

[Close]

BLAST of CSPI01G09010 vs. ExPASy TrEMBL
Match: A0A5A7ULX7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001290 PE=4 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 2.2e-164
Identity = 282/319 (88.40%), Postives = 300/319 (94.04%), Query Frame = 0


Query: 18  MLLIHSLSFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHVAPGTYHETLQIPRLKKF 77
           ML +HS+SFNLTVSLDGSGNFKRISDAI AAPNNSNTRFYIHVAPGTYHE +QIP+ KKF
Sbjct: 1   MLFVHSISFNLTVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKF 60

Query: 78  IALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQAVAVF 137
           IAL+GDD FTTIIIDNRSNGTGFKTNNSATLTVQGDNFMA+SLTFENSAGPQN QAVAVF
Sbjct: 61  IALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAKSLTFENSAGPQNGQAVAVF 120

Query: 138 DKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIYARMPI 197
           DKA+HTAYYKCRFL FQDTLYVNGK QFFKES+IYGSVDFI G+G+VMFQDCNIYARMP 
Sbjct: 121 DKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPS 180

Query: 198 NSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVFMESFL 257
           NSITVTAQSK  LRS+SGFSFQNCTVTVS EISSNKQNVKVFLGRPW+QYS VVFMESFL
Sbjct: 181 NSITVTAQSKDFLRSISGFSFQNCTVTVSPEISSNKQNVKVFLGRPWRQYSNVVFMESFL 240

Query: 258 DDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTSYHLLDKESALRFTVDNFV 317
           DDVVASEGW+EW GVPVNNLFYGEFNN GPGADVSKRVNWT YHLLDK+SA+ FTVDNF+
Sbjct: 241 DDVVASEGWMEWKGVPVNNLFYGEFNNFGPGADVSKRVNWTGYHLLDKKSAIGFTVDNFI 300

Query: 318 NGSEWLPETGIPFRRGLLS 337
           +GSEWL ETGIPFRRGLL+
Sbjct: 301 DGSEWLAETGIPFRRGLLT 319

[Close]

BLAST of CSPI01G09010 vs. ExPASy TrEMBL
Match: A0A5D3BM28 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004180 PE=4 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 9.6e-160
Identity = 276/319 (86.52%), Postives = 295/319 (92.48%), Query Frame = 0


Query: 18  MLLIHSLSFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHVAPGTYHETLQIPRLKKF 77
           ML +HS+SFNLTVSLDGSGNFKRISDAI AAPNNSNTRFYIHVAPGTYHE +QIP+ KKF
Sbjct: 1   MLFVHSISFNLTVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKF 60

Query: 78  IALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQAVAVF 137
           IAL+GDD FTTIIIDNRSNGTGFKTNNSATLTVQG+NFMA+SLTFENSAGPQN QAVAVF
Sbjct: 61  IALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVF 120

Query: 138 DKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIYARMPI 197
           DKA+HTAYYKCRFL FQDTLYVNGK QFFKES+IYGSVDFI G+GQVMFQDCNIYARMP 
Sbjct: 121 DKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGQVMFQDCNIYARMPS 180

Query: 198 NSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVFMESFL 257
           NSITVTAQSK  LRS+SGFSFQN TVTV  EISSNKQNVKVFLGR W+QYS VVFMESFL
Sbjct: 181 NSITVTAQSKDFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFL 240

Query: 258 DDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTSYHLLDKESALRFTVDNFV 317
           DDVVASEGW+EW GVP+NNLFYGEFNN GPGADVSKRVNWT YH+L+KESA RFTVDNF+
Sbjct: 241 DDVVASEGWMEWKGVPINNLFYGEFNNYGPGADVSKRVNWTGYHILNKESAFRFTVDNFI 300

Query: 318 NGSEWLPETGIPFRRGLLS 337
           N S+WL ETGIPFR GLL+
Sbjct: 301 NRSKWLHETGIPFRHGLLT 319

[Close]

BLAST of CSPI01G09010 vs. ExPASy TrEMBL
Match: A0A5A7UNS3 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001280 PE=4 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 3.3e-136
Identity = 236/275 (85.82%), Postives = 253/275 (92.00%), Query Frame = 0


Query: 62  PGTYHETLQIPRLKKFIALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLT 121
           PGTYHE +QIP+ KKFIAL+GDD FTTIIIDNRSNGTGFKTNNSATLTVQG+NFMA+SLT
Sbjct: 7   PGTYHEAIQIPKAKKFIALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLT 66

Query: 122 FENSAGPQNNQAVAVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGY 181
           FENSAGPQN QAVAVFDKA+HTAYYKCRFL FQDTLYVNGK QFFKES+IYGSVDFI G+
Sbjct: 67  FENSAGPQNGQAVAVFDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGH 126

Query: 182 GQVMFQDCNIYARMPINSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFLG 241
           GQVMFQDCNIYARMP NSITVTAQSK  LRS+SGFSFQN TVTV  EISSNKQNVKVFLG
Sbjct: 127 GQVMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVLLEISSNKQNVKVFLG 186

Query: 242 RPWKQYSKVVFMESFLDDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTSYH 301
           R W+QYS VVFMESFLDDVVASEGW+EW GVP+ NLFYGEFNN GPGADVSKRVNWT YH
Sbjct: 187 RLWRQYSNVVFMESFLDDVVASEGWMEWKGVPIKNLFYGEFNNYGPGADVSKRVNWTGYH 246

Query: 302 LLDKESALRFTVDNFVNGSEWLPETGIPFRRGLLS 337
           +L+KESA RFTVDNF+NGS+WL ETGIPFR GLL+
Sbjct: 247 ILNKESAFRFTVDNFINGSKWLHETGIPFRHGLLT 281

[Close]

BLAST of CSPI01G09010 vs. ExPASy TrEMBL
Match: A0A1S3CP85 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503231 PE=4 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 1.6e-135
Identity = 235/275 (85.45%), Postives = 253/275 (92.00%), Query Frame = 0


Query: 62  PGTYHETLQIPRLKKFIALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLT 121
           PGTYHE +QIP+ KKFIAL+GDD FTTIIIDNRSNGTGFKTNNSATLTVQG+NFMA+SLT
Sbjct: 1   PGTYHEAIQIPKAKKFIALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLT 60

Query: 122 FENSAGPQNNQAVAVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGY 181
           FENSAGPQN QAVAVFDKA+HTAYYKCRFL FQDTLYVNGK QFFKES+IYGSVDFI G+
Sbjct: 61  FENSAGPQNGQAVAVFDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGH 120

Query: 182 GQVMFQDCNIYARMPINSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFLG 241
           G+VMFQDCNIYARMP NSITVTAQSK  LRS+SGFSFQN TVTV  EISSNKQNVKVFLG
Sbjct: 121 GRVMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLG 180

Query: 242 RPWKQYSKVVFMESFLDDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTSYH 301
           R W+QYS VVFMESFLDDVVASEGW+EW GVP+NNLFYGEFNN GPGADVSKRVNWT YH
Sbjct: 181 RLWRQYSNVVFMESFLDDVVASEGWMEWKGVPINNLFYGEFNNYGPGADVSKRVNWTGYH 240

Query: 302 LLDKESALRFTVDNFVNGSEWLPETGIPFRRGLLS 337
           +L+KESA RFTVDNF+N S+WL ETGIPFR GLL+
Sbjct: 241 ILNKESAFRFTVDNFINRSKWLHETGIPFRHGLLT 275

[Close]

BLAST of CSPI01G09010 vs. NCBI nr
Match: XP_004152582.2 (pectinesterase 2 [Cucumis sativus] >KGN64355.1 hypothetical protein Csa_013871 [Cucumis sativus])

HSP 1 Score: 688.0 bits (1774), Expect = 4.2e-194
Identity = 335/336 (99.70%), Postives = 335/336 (99.70%), Query Frame = 0


Query: 1   MLFFFGVPCSLFCFFNLMLLIHSLSFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHV 60
           MLFFFGVPCSLFCFFNLMLLIHSLSFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHV
Sbjct: 1   MLFFFGVPCSLFCFFNLMLLIHSLSFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHV 60

Query: 61  APGTYHETLQIPRLKKFIALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESL 120
           APGTYHETLQIPRLKKFIALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESL
Sbjct: 61  APGTYHETLQIPRLKKFIALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESL 120

Query: 121 TFENSAGPQNNQAVAVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICG 180
           TFENSAGPQNNQAVAVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICG
Sbjct: 121 TFENSAGPQNNQAVAVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICG 180

Query: 181 YGQVMFQDCNIYARMPINSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFL 240
           YGQVMFQDCNIYARMPINSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFL
Sbjct: 181 YGQVMFQDCNIYARMPINSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFL 240

Query: 241 GRPWKQYSKVVFMESFLDDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTSY 300
           GRPWKQYSKVVFMESFLDDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTSY
Sbjct: 241 GRPWKQYSKVVFMESFLDDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTSY 300

Query: 301 HLLDKESALRFTVDNFVNGSEWLPETGIPFRRGLLS 337
           HLLDKESALRFTVDNFVNGSEWLPETGIPFRRGL S
Sbjct: 301 HLLDKESALRFTVDNFVNGSEWLPETGIPFRRGLHS 336

[Close]

BLAST of CSPI01G09010 vs. NCBI nr
Match: KAA0055286.1 (putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa])

HSP 1 Score: 588.2 bits (1515), Expect = 4.5e-164
Identity = 282/319 (88.40%), Postives = 300/319 (94.04%), Query Frame = 0


Query: 18  MLLIHSLSFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHVAPGTYHETLQIPRLKKF 77
           ML +HS+SFNLTVSLDGSGNFKRISDAI AAPNNSNTRFYIHVAPGTYHE +QIP+ KKF
Sbjct: 1   MLFVHSISFNLTVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKF 60

Query: 78  IALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQAVAVF 137
           IAL+GDD FTTIIIDNRSNGTGFKTNNSATLTVQGDNFMA+SLTFENSAGPQN QAVAVF
Sbjct: 61  IALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAKSLTFENSAGPQNGQAVAVF 120

Query: 138 DKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIYARMPI 197
           DKA+HTAYYKCRFL FQDTLYVNGK QFFKES+IYGSVDFI G+G+VMFQDCNIYARMP 
Sbjct: 121 DKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPS 180

Query: 198 NSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVFMESFL 257
           NSITVTAQSK  LRS+SGFSFQNCTVTVS EISSNKQNVKVFLGRPW+QYS VVFMESFL
Sbjct: 181 NSITVTAQSKDFLRSISGFSFQNCTVTVSPEISSNKQNVKVFLGRPWRQYSNVVFMESFL 240

Query: 258 DDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTSYHLLDKESALRFTVDNFV 317
           DDVVASEGW+EW GVPVNNLFYGEFNN GPGADVSKRVNWT YHLLDK+SA+ FTVDNF+
Sbjct: 241 DDVVASEGWMEWKGVPVNNLFYGEFNNFGPGADVSKRVNWTGYHLLDKKSAIGFTVDNFI 300

Query: 318 NGSEWLPETGIPFRRGLLS 337
           +GSEWL ETGIPFRRGLL+
Sbjct: 301 DGSEWLAETGIPFRRGLLT 319

[Close]

BLAST of CSPI01G09010 vs. NCBI nr
Match: TYJ99208.1 (putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa])

HSP 1 Score: 572.8 bits (1475), Expect = 2.0e-159
Identity = 276/319 (86.52%), Postives = 295/319 (92.48%), Query Frame = 0


Query: 18  MLLIHSLSFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHVAPGTYHETLQIPRLKKF 77
           ML +HS+SFNLTVSLDGSGNFKRISDAI AAPNNSNTRFYIHVAPGTYHE +QIP+ KKF
Sbjct: 1   MLFVHSISFNLTVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKF 60

Query: 78  IALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQAVAVF 137
           IAL+GDD FTTIIIDNRSNGTGFKTNNSATLTVQG+NFMA+SLTFENSAGPQN QAVAVF
Sbjct: 61  IALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAVF 120

Query: 138 DKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIYARMPI 197
           DKA+HTAYYKCRFL FQDTLYVNGK QFFKES+IYGSVDFI G+GQVMFQDCNIYARMP 
Sbjct: 121 DKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGQVMFQDCNIYARMPS 180

Query: 198 NSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVFMESFL 257
           NSITVTAQSK  LRS+SGFSFQN TVTV  EISSNKQNVKVFLGR W+QYS VVFMESFL
Sbjct: 181 NSITVTAQSKDFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESFL 240

Query: 258 DDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTSYHLLDKESALRFTVDNFV 317
           DDVVASEGW+EW GVP+NNLFYGEFNN GPGADVSKRVNWT YH+L+KESA RFTVDNF+
Sbjct: 241 DDVVASEGWMEWKGVPINNLFYGEFNNYGPGADVSKRVNWTGYHILNKESAFRFTVDNFI 300

Query: 318 NGSEWLPETGIPFRRGLLS 337
           N S+WL ETGIPFR GLL+
Sbjct: 301 NRSKWLHETGIPFRHGLLT 319

[Close]

BLAST of CSPI01G09010 vs. NCBI nr
Match: KAA0055285.1 (putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa])

HSP 1 Score: 494.6 bits (1272), Expect = 6.8e-136
Identity = 236/275 (85.82%), Postives = 253/275 (92.00%), Query Frame = 0


Query: 62  PGTYHETLQIPRLKKFIALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLT 121
           PGTYHE +QIP+ KKFIAL+GDD FTTIIIDNRSNGTGFKTNNSATLTVQG+NFMA+SLT
Sbjct: 7   PGTYHEAIQIPKAKKFIALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLT 66

Query: 122 FENSAGPQNNQAVAVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGY 181
           FENSAGPQN QAVAVFDKA+HTAYYKCRFL FQDTLYVNGK QFFKES+IYGSVDFI G+
Sbjct: 67  FENSAGPQNGQAVAVFDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGH 126

Query: 182 GQVMFQDCNIYARMPINSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFLG 241
           GQVMFQDCNIYARMP NSITVTAQSK  LRS+SGFSFQN TVTV  EISSNKQNVKVFLG
Sbjct: 127 GQVMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVLLEISSNKQNVKVFLG 186

Query: 242 RPWKQYSKVVFMESFLDDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTSYH 301
           R W+QYS VVFMESFLDDVVASEGW+EW GVP+ NLFYGEFNN GPGADVSKRVNWT YH
Sbjct: 187 RLWRQYSNVVFMESFLDDVVASEGWMEWKGVPIKNLFYGEFNNYGPGADVSKRVNWTGYH 246

Query: 302 LLDKESALRFTVDNFVNGSEWLPETGIPFRRGLLS 337
           +L+KESA RFTVDNF+NGS+WL ETGIPFR GLL+
Sbjct: 247 ILNKESAFRFTVDNFINGSKWLHETGIPFRHGLLT 281

[Close]

BLAST of CSPI01G09010 vs. NCBI nr
Match: XP_008465596.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 47, partial [Cucumis melo])

HSP 1 Score: 492.3 bits (1266), Expect = 3.4e-135
Identity = 235/275 (85.45%), Postives = 253/275 (92.00%), Query Frame = 0


Query: 62  PGTYHETLQIPRLKKFIALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLT 121
           PGTYHE +QIP+ KKFIAL+GDD FTTIIIDNRSNGTGFKTNNSATLTVQG+NFMA+SLT
Sbjct: 1   PGTYHEAIQIPKAKKFIALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLT 60

Query: 122 FENSAGPQNNQAVAVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGY 181
           FENSAGPQN QAVAVFDKA+HTAYYKCRFL FQDTLYVNGK QFFKES+IYGSVDFI G+
Sbjct: 61  FENSAGPQNGQAVAVFDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGH 120

Query: 182 GQVMFQDCNIYARMPINSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFLG 241
           G+VMFQDCNIYARMP NSITVTAQSK  LRS+SGFSFQN TVTV  EISSNKQNVKVFLG
Sbjct: 121 GRVMFQDCNIYARMPSNSITVTAQSKDFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLG 180

Query: 242 RPWKQYSKVVFMESFLDDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTSYH 301
           R W+QYS VVFMESFLDDVVASEGW+EW GVP+NNLFYGEFNN GPGADVSKRVNWT YH
Sbjct: 181 RLWRQYSNVVFMESFLDDVVASEGWMEWKGVPINNLFYGEFNNYGPGADVSKRVNWTGYH 240

Query: 302 LLDKESALRFTVDNFVNGSEWLPETGIPFRRGLLS 337
           +L+KESA RFTVDNF+N S+WL ETGIPFR GLL+
Sbjct: 241 ILNKESAFRFTVDNFINRSKWLHETGIPFRHGLLT 275

[Close]

BLAST of CSPI01G09010 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 280.0 bits (715), Expect = 2.5e-75
Identity = 136/308 (44.16%), Postives = 197/308 (63.96%), Query Frame = 0


Query: 35  SGNFKRISDAIAAAPNNSNTR---FYIHVAPGTYHETLQIPRLKKFIALVGDDPFTTIII 94
           S NF  I+DAIAAAPNN+      F I+   G Y E + +P  KK + L+GD    TII 
Sbjct: 310 SDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIIT 369

Query: 95  DNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQAVAVFDKANHTAYYKCRFL 154
            N +   G+ T N ++  V G+ FMA  +TF N+AGP+ +QAVA+ + A  +++Y+C F 
Sbjct: 370 GNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFE 429

Query: 155 SFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIYARMPI--NSITVTAQSKYI 214
            +QDTLYV+   QF++E DIYG+VDFI G    +FQ+CNIYAR P+      +TA  +  
Sbjct: 430 GYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLD 489

Query: 215 LRSVSGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVFMESFLDDVVASEGWVEW 274
               +G S  NCT+  + ++++  ++   FLGRPWK YS+ VFM+S++ D+V   GW+EW
Sbjct: 490 PNQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEW 549

Query: 275 IG-VPVNNLFYGEFNNCGPGADVSKRVNWTSYHLLDKESALRFTVDNFVNGSEWLPETGI 334
            G + ++ ++YGE++N GPGA+ ++RV W  Y+LL+   A+ FTV NF  G  WLP+T I
Sbjct: 550 NGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQTDI 609

Query: 335 PFRRGLLS 337
           PF  GLLS
Sbjct: 610 PFYGGLLS 617

[Close]

BLAST of CSPI01G09010 vs. TAIR 10
Match: AT2G26440.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 279.6 bits (714), Expect = 3.2e-75
Identity = 132/313 (42.17%), Postives = 194/313 (61.98%), Query Frame = 0


Query: 25  SFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHVAPGTYHETLQIPRLKKFIALVGDD 84
           S +L V+ DG+GNF  I++AI+ APN SN R  I+V  G Y E + IP  K  I L+GD 
Sbjct: 235 SESLVVAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDG 294

Query: 85  PFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQAVAVFDKANHTA 144
              T I  NRS G G+ T  SATL V G+ F+A  +   N+AGP+ +QAVA+   A+  A
Sbjct: 295 SDVTFITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVA 354

Query: 145 YYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIYARMPI--NSITV 204
            Y+C    +QDTLY +   QF++E DIYG++D+I G   V+FQ CNI +++P+      +
Sbjct: 355 LYRCVIDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVI 414

Query: 205 TAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVFMESFLDDVVA 264
           TAQS+      +G S QNC++  S ++ ++   VK +LGRPW+++S+ V MES++D+ + 
Sbjct: 415 TAQSRDTQDEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMESYIDEFID 474

Query: 265 SEGWVEWI-GVPVNNLFYGEFNNCGPGADVSKRVNWTSYHLLDKESALRFTVDNFVNGSE 324
             GW +W  G  ++ L+YGE+NN GPG++  KRVNW  +H++  E A  FT   F+ G  
Sbjct: 475 GSGWSKWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIMGYEDAFNFTATEFITGDG 534

Query: 325 WLPETGIPFRRGL 335
           WL  T  P+  G+
Sbjct: 535 WLGSTSFPYDNGI 547

[Close]

BLAST of CSPI01G09010 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 279.3 bits (713), Expect = 4.2e-75
Identity = 136/325 (41.85%), Postives = 207/325 (63.69%), Query Frame = 0


Query: 18  MLLIHSLSFNLTVSLDGSGNFKRISDAIAAAPNNSNTR---FYIHVAPGTYHETLQIPRL 77
           M+LI  +   +TVS DG+GNF  I+ A+AAAPNN++     F I+V  G Y E + I + 
Sbjct: 258 MVLISDI---VTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKN 317

Query: 78  KKFIALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQAV 137
           K+++ ++GD    T++  NRS   G+ T NSAT  V   NF+A ++TF N+AGP+ +QAV
Sbjct: 318 KRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAV 377

Query: 138 AVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIYAR 197
           A+   A+ + +Y C F ++QDTLY +   QF++E D+YG+VDFI G   V+FQ+CN+Y R
Sbjct: 378 ALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPR 437

Query: 198 --MPINSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVF 257
             MP     +TAQ +      +G S QNCT+  + ++ S+   VK +LGRPWK+YS+ V+
Sbjct: 438 KPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVY 497

Query: 258 MESFLDDVVASEGWVEWIG-VPVNNLFYGEFNNCGPGADVSKRVNWTSYHLLDKESALRF 317
           M+S++D  V   GW EW G   ++ L+Y E+NN GPG++ + RV W  YH+++   A  F
Sbjct: 498 MQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAANF 557

Query: 318 TVDNFVNGSEWLPETGIPFRRGLLS 337
           TV      ++W+ +TG+P+  GL+S
Sbjct: 558 TVTGLFIEADWIWKTGVPYTSGLIS 579

[Close]

BLAST of CSPI01G09010 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 278.9 bits (712), Expect = 5.5e-75
Identity = 131/315 (41.59%), Postives = 204/315 (64.76%), Query Frame = 0


Query: 28  LTVSLDGSGNFKRISDAIAAAPNNSNTR---FYIHVAPGTYHETLQIPRLKKFIALVGDD 87
           +TV+ +G+GNF  I++A+ +APN ++     F I+V  G Y E + I + K+++ ++GD 
Sbjct: 259 VTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKRYLMMIGDG 318

Query: 88  PFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQAVAVFDKANHTA 147
              T++  NR+   G+ T NSAT  V   NF+A ++TF N+AGP+ +QAVA+   A+ + 
Sbjct: 319 INRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAMRSSADLSI 378

Query: 148 YYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIYARMPINS--ITV 207
           +Y C F ++QDTLY +   QF++E DIYG+VDFI G   V+FQDCN+Y R P+ +    +
Sbjct: 379 FYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPMQNQFNAI 438

Query: 208 TAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVFMESFLDDVVA 267
           TAQ +      +G S  NCT+  + ++ S+   VK +LGRPWK+YS+ VFM+S++D+VV 
Sbjct: 439 TAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVE 498

Query: 268 SEGWVEWIG-VPVNNLFYGEFNNCGPGADVSKRVNWTSYHLLDKESALRFTVDNFVNGSE 327
             GW EW G   ++ L+Y E+NN G G+  + RV W  YH+++   A  FTV+NF+ G  
Sbjct: 499 PVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFTVENFLLGDG 558

Query: 328 WLPETGIPFRRGLLS 337
           W+ ++G+P+  GLLS
Sbjct: 559 WMVQSGVPYISGLLS 573

[Close]

BLAST of CSPI01G09010 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 276.9 bits (707), Expect = 2.1e-74
Identity = 133/317 (41.96%), Postives = 206/317 (64.98%), Query Frame = 0


Query: 21  IHSLSFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIHVAPGTYHETLQIPRLKKFIAL 80
           +   ++NL+V++DG+GNF  I+DA+ AAPN S TRF I++  G Y E +++P+ K  I  
Sbjct: 215 VQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMF 274

Query: 81  VGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQAVAVFDKA 140
           +GD    T+I  NRS   G+ T  + T+ V+G  ++A+ ++F NSAGP   QAVA    +
Sbjct: 275 IGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGS 334

Query: 141 NHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIYARMPI--N 200
           +H+A+Y+C F  +QDTLYV+   QF++E DIYG++DFI G   V+FQ+ ++YAR P   +
Sbjct: 335 DHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGH 394

Query: 201 SITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVFMESFLD 260
            I  TAQS+      +G S  NC +  + ++   K+N K +LGRPW++YS+ V ++SF+D
Sbjct: 395 KIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFID 454

Query: 261 DVVASEGWVEW-IGVPVNNLFYGEFNNCGPGADVSKRVNWTSYHLLDKES-ALRFTVDNF 320
           D++   GW+E      +  L+YGE+ N GPGA+++KRV W  +  ++ ++ A +FTV  F
Sbjct: 455 DLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPF 514

Query: 321 VNGSEWLPETGIPFRRG 334
           ++GS WL  TGIPF  G
Sbjct: 515 IDGSTWLNSTGIPFSLG 531

The following BLAST results are available for this feature:

BLAST of CSPI01G09010 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04887	1.1e-75	45.02	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
Q94CB1	3.5e-74	44.16	Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3...	[more]
O48711	4.5e-74	42.17	Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3...	[more]
Q9SRX4	5.9e-74	41.85	Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37...	[more]
Q8RXK7	7.7e-74	41.59	Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of CSPI01G09010 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LR14	2.0e-194	99.70	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G049450 PE=4 SV=1	[more]
A0A5A7ULX7	2.2e-164	88.40	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001290...	[more]
A0A5D3BM28	9.6e-160	86.52	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00418...	[more]
A0A5A7UNS3	3.3e-136	85.82	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001280...	[more]
A0A1S3CP85	1.6e-135	85.45	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503231 PE=4 SV=1	[more]

BLAST of CSPI01G09010 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152582.2	4.2e-194	99.70	pectinesterase 2 [Cucumis sativus] >KGN64355.1 hypothetical protein Csa_013871 [...	[more]
KAA0055286.1	4.5e-164	88.40	putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa]	[more]
TYJ99208.1	2.0e-159	86.52	putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa]	[more]
KAA0055285.1	6.8e-136	85.82	putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa]	[more]
XP_008465596.1	3.4e-135	85.45	PREDICTED: probable pectinesterase/pectinesterase inhibitor 47, partial [Cucumis...	[more]

BLAST of CSPI01G09010 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10720.2	2.5e-75	44.16	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G26440.1	3.2e-75	42.17	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G02810.1	4.2e-75	41.85	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02330.1	5.5e-75	41.59	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02300.1	2.1e-74	41.96	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 27..319 e-value: 1.8E-100 score: 335.5
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 22..334 e-value: 7.1E-110 score: 369.3
None	No IPR available	PANTHER	PTHR31707:SF149	PECTINESTERASE/PECTINESTERASE INHIBITOR 17-RELATED	coord: 24..331
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 24..331
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 25..332

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G09010.1	CSPI01G09010.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity