CSPI01G08440 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G08440
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	E3 ubiquitin-protein ligase SIS3-like
Location	Chr1: 5294711 .. 5298399 (+)
RNA-Seq Expression	CSPI01G08440
Synteny	CSPI01G08440

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTTGTTCATTTGAACTAGTTTTAACTTAAATTTCCAAATGAAGAATGGTTAGAGGAAAGTTGTTTGATTTGAAATTTACTTTCCTTTTTACAAGACAATGGAAAAAGGGAATAAGGAAAAGAAAAAAAGAAAAGTTGGATGCATACTTTAATAAGGGTAGATATTTGAATTTGCAGACTTGTGGTTACAATTATGCTCTGAAATGGCAGTCATTACGCTTTACAGTTGTACAAGCAACGCTGCAGAGAAATCAGATTACTGCTCAACCCCCATGGCCGAACGGGATCCATTCCATTCCAGGCTTCTTCATCACTATAGTCTTCTCTTTTTCCATCTCTCTGTTCATCAATGATTCAACCCCAGAAAGAATTTCTTGCCAAAAATTCAACTTCTTCTTTGATTTTCCTTTCCCTTCTGGTTCTTCCATCGTTTTCCTACCCTCACATGGCGGCCACCCTTTAGCCATTTTTGGCATCTCCGATTGTTGTTTGGGTTTCTTGTTTTAGTTTTTGGATATGGCTATTCGAGGTGTCGATTTCAAATGGTAAATCTCTTCCCCCCCATCGTTTTATTGGAATCCAAATTTACCCTTATTCATGGGGTTTTCACTTCTTCAGTATTTTTGGGATTTTTCAGGTACGATGGCTTCTTCTTGTCTATGCTTGCAACTAGTATGTATCCTTTTGCCATATGACTGCAAGCTGTTGTTATTTAGGTTTGCTTGTTTTAACTCTTTTCAGAAAATTGTAATTGGATTTCATGAGCCTTCTCTTGCATTCCTTCTGAGATCAAGCTGTTCAACTAGAAACTTTGTTTCTTGTTTATGTTACTTTTTTTCTTGCTTTCCTTTAACCCCCTGCTTGTGTTTTTTACTTAGCTTTTTGAGCGCTGGAATGATTATATATGGGGAATAAGTTTCATTGTTATATTTCTTTATCTCTTCTTTTCTTTAACTGTTTTGCTTGTTAGAATCATTGTGACCATCAATTGGAGAAGGTACAATACTTGTACATATCCTTTGCACATATGGATTGTGGTATGTGTCCAGGTTTTTAAAAAAGTGATACAATTCTTAAGTTATATTTACGATTGCTTGCTTCTGATGGCTATGTATATCACTTTCTTCATTCAGGTTGATTATACCTCTGTGTTTGCTTTTCGCCTTTTGTTGTTCATAGATAATGCACTTTCAGCTGGAATGGGATTGTAAGGCTTTCTCCATTTATCTCTCTATATATAATGCACTTGTGGTAGAGGCACTTAAATTCTTCTGTTTACAGTATATTTTAGATTCTTAAGGGTTGTCTTATTCTTACTGTTGAATTTAAATTGCGTTAGAGATTTTGGATGGCAGCAGCGGTATGCTCGGTTTTGTGGAAGAGTCGTGGTTCTTTCTATCCTGTCTCTATTGCTCTACCCGTTTCTATGTGCGTGGACTGTGATTGGTACCCTGTGGTTTAGAGATGCTAAAGACTGTGTAAGCTATATTTCCCTTAGTTAACATTGGCCAAATCATCTATTTACAAGCTTTGATTATTTAGTTTGTGTTATTGATGCAAGCAGTTACCTGAAGAGGGACAGAAATGGGGTTTCCTTATTTGGTTGCTTTTCAGCTACTGTGCCCTCCTTTGCATTGCCTGCATGTCAGTTGGAAAGGTTATACATTTTCTGTGTACTTCTCCATTCTCTTGCTTAAATCTTGCCTGTTTGTTACTTTAGTGAATTTTAAGCAAGTTTAGGCTCTTAATTGAGGGCCTGAGGCGCATATTATGTTACAGAGCTATTCCTTAAAGTCTGTTTCTAACTTGATCTTTTCTGTTAACCACATACTTAGTGTTTCAAATTATTCTTGTTTGATACTAACCGCTTTTCTTGTCGGTTCTTAATCCGTCTTATGTGCAGTGGTTAGTGCGTAGACAAGCACACTTGTTCCGTGCCCAACAAGGCATACCGGTATCAGAATATGGAGTAAGCTTTAAACTTTTTGGTGTTAAACCAAGGAATAATAGTTGTTTTTGTCCAACAAGGCATATATCTTCTTGTTTTGCTTTTTTCGGCATTGCTATACACTTTATTTTATAAAATGCCCTACCTTCAAAAGTGCAAAATTTCTTTGATTTGGGATCATATGCTCTTTTTCTTTACAAGTGTATCAGTTTATAACCATCAAACATTGAATTGACTAGAATTTTTCTGCATACAGGTTCTAGTTGATATGATTAGAGTTCCTGATTGGGCATATGAAGCTGCAGGACTGGAAACACGAGCCATTGGCCAAGATGCTACTGGGTATCATCCCGGACTGTTCTTAACCTCAGCTCAGGTAAATGTCTGAAGCGTAAATCGGTGCATATGGAAATCTTTTGCCTTCAGCTATGGATCTGTTGTGATAACATGTATTCAAGTAATTTCACTTGTCTATTGACTTCTGTAACCTTTGTTTTGCAAACGGGATGTTAAGGTCCTGTAGCATATTACACTCGCAAGTTCAAGATTAATGTGGGAGGAAATCTGGATGGAACTTCTTTACCTTAAGAATGTGTACTGGAAAATCTGTAGGATTGCTCTTTCTTTGCTTATTATGAAGCAGTGGTTTGTTTTATTGTAGTCAATAGTCGTTGGCACAAAATTTTAAGAAACGGGCGACTTTTGTGCAGCGGGAAGCTGTGGAAGCTCTCATCCAGGAACTTCCAAAGTTTAGGCTGAAGGCTGTCCCAACTGATTGCAGTGAATGCCCTATTTGCCTTGAAGAGTTTCATGTAGGGAATGAGGTAAACTGTCGGCTTCATATTTTCAATCATTCATCTCAAAATGTTTACATTGCACATGTTCTCTTATTTTGAACTTGAGAAACCAAGCTCAATTTATGCAGTAACCTACCTACGATCTAATACCTTACGAGTTTTCTCAGCACCCAAATATTGTAATGTTACGTGGGTTACCCAAAGATTAGTCAAAGTATGCCCAAGCTGGTTTGGGATAGTCAATGGATATATGTAAATAAATAAAAAAACGTTTTGGTGCAGTTCTTATTGGTTTTTACTTTGTGCAGGTTCGTGGTATGCCATGTGCTCACAATTTCCACGTTGAATGTATTGACGAATGGCTTCGTCTGAATGTCAAATGTCCTCGATGCCGCTCCTCCGTCTTCCCAAATCTCGACCTCAGCGCTCTTTCTAACCTGCATTCAGACACAGAACAAACATCTCACAATGCTTTTGTAACAACCCAACCTTCAAGTCTATCGAGATCAACTCCAACTCACGCAGCTCATACCGAAAACAGTCTCCAAACTGATACTGCCTTAGATGCTTTGGAAAATGGAAACTCACCCATGCCATCTGCTTCTCCTCATTGATCTACAACCATCTTAACCAAAACTCCTCAGAAAGATTCAACACTGATATAAACCAGAAAAAGCAAAAGAACACTGACATCTTCTATAGTTGTTCTATTGTAAATCCTTCTAAAAACTTGGCCAAAGAGTGAATTTTTTGTTAGGTTTTTGAAAGATGGTTATAAAATATTTATGAATCTGACAGCGACCCTCAAAATTTTAGCCATGGAGGAAACAAGAGTTCACGTGGGTTTCTTCTGTTAGGCCATGTTCATGGCTCGTGGATCTCATAGATTCCTCACCACTTGACAAATATTTCCCATCAGATCCTCCCTTTCCATTTCCAACCC

mRNA sequence

AATTTGTTCATTTGAACTAGTTTTAACTTAAATTTCCAAATGAAGAATGGTTAGAGGAAAGTTGTTTGATTTGAAATTTACTTTCCTTTTTACAAGACAATGGAAAAAGGGAATAAGGAAAAGAAAAAAAGAAAAGTTGGATGCATACTTTAATAAGGGTAGATATTTGAATTTGCAGACTTGTGGTTACAATTATGCTCTGAAATGGCAGTCATTACGCTTTACAGTTGTACAAGCAACGCTGCAGAGAAATCAGATTACTGCTCAACCCCCATGGCCGAACGGGATCCATTCCATTCCAGGCTTCTTCATCACTATAGTCTTCTCTTTTTCCATCTCTCTGTTCATCAATGATTCAACCCCAGAAAGAATTTCTTGCCAAAAATTCAACTTCTTCTTTGATTTTCCTTTCCCTTCTGGTTCTTCCATCGTTTTCCTACCCTCACATGGCGGCCACCCTTTAGCCATTTTTGGCATCTCCGATTGTTGTTTGGGTTTCTTGTTTTAGTTTTTGGATATGGCTATTCGAGGTGTCGATTTCAAATGGTACGATGGCTTCTTCTTGTCTATGCTTGCAACTAGTATAATCATTGTGACCATCAATTGGAGAAGGTACAATACTTGTACATATCCTTTGCACATATGGATTGTGGTTGATTATACCTCTGTGTTTGCTTTTCGCCTTTTGTTGTTCATAGATAATGCACTTTCAGCTGGAATGGGATTAGATTTTGGATGGCAGCAGCGGTATGCTCGGTTTTGTGGAAGAGTCGTGGTTCTTTCTATCCTGTCTCTATTGCTCTACCCGTTTCTATGTGCGTGGACTGTGATTGGTACCCTGTGGTTTAGAGATGCTAAAGACTGTTTACCTGAAGAGGGACAGAAATGGGGTTTCCTTATTTGGTTGCTTTTCAGCTACTGTGCCCTCCTTTGCATTGCCTGCATGTCAGTTGGAAAGTGGTTAGTGCGTAGACAAGCACACTTGTTCCGTGCCCAACAAGGCATACCGGTATCAGAATATGGAGTTCTAGTTGATATGATTAGAGTTCCTGATTGGGCATATGAAGCTGCAGGACTGGAAACACGAGCCATTGGCCAAGATGCTACTGGGTATCATCCCGGACTGTTCTTAACCTCAGCTCAGCGGGAAGCTGTGGAAGCTCTCATCCAGGAACTTCCAAAGTTTAGGCTGAAGGCTGTCCCAACTGATTGCAGTGAATGCCCTATTTGCCTTGAAGAGTTTCATGTAGGGAATGAGGTTCGTGGTATGCCATGTGCTCACAATTTCCACGTTGAATGTATTGACGAATGGCTTCGTCTGAATGTCAAATGTCCTCGATGCCGCTCCTCCGTCTTCCCAAATCTCGACCTCAGCGCTCTTTCTAACCTGCATTCAGACACAGAACAAACATCTCACAATGCTTTTGTAACAACCCAACCTTCAAGTCTATCGAGATCAACTCCAACTCACGCAGCTCATACCGAAAACAGTCTCCAAACTGATACTGCCTTAGATGCTTTGGAAAATGGAAACTCACCCATGCCATCTGCTTCTCCTCATTGATCTACAACCATCTTAACCAAAACTCCTCAGAAAGATTCAACACTGATATAAACCAGAAAAAGCAAAAGAACACTGACATCTTCTATAGTTGTTCTATTGTAAATCCTTCTAAAAACTTGGCCAAAGAGTGAATTTTTTGTTAGGTTTTTGAAAGATGGTTATAAAATATTTATGAATCTGACAGCGACCCTCAAAATTTTAGCCATGGAGGAAACAAGAGTTCACGTGGGTTTCTTCTGTTAGGCCATGTTCATGGCTCGTGGATCTCATAGATTCCTCACCACTTGACAAATATTTCCCATCAGATCCTCCCTTTCCATTTCCAACCC

Coding sequence (CDS)

ATGGCTATTCGAGGTGTCGATTTCAAATGGTACGATGGCTTCTTCTTGTCTATGCTTGCAACTAGTATAATCATTGTGACCATCAATTGGAGAAGGTACAATACTTGTACATATCCTTTGCACATATGGATTGTGGTTGATTATACCTCTGTGTTTGCTTTTCGCCTTTTGTTGTTCATAGATAATGCACTTTCAGCTGGAATGGGATTAGATTTTGGATGGCAGCAGCGGTATGCTCGGTTTTGTGGAAGAGTCGTGGTTCTTTCTATCCTGTCTCTATTGCTCTACCCGTTTCTATGTGCGTGGACTGTGATTGGTACCCTGTGGTTTAGAGATGCTAAAGACTGTTTACCTGAAGAGGGACAGAAATGGGGTTTCCTTATTTGGTTGCTTTTCAGCTACTGTGCCCTCCTTTGCATTGCCTGCATGTCAGTTGGAAAGTGGTTAGTGCGTAGACAAGCACACTTGTTCCGTGCCCAACAAGGCATACCGGTATCAGAATATGGAGTTCTAGTTGATATGATTAGAGTTCCTGATTGGGCATATGAAGCTGCAGGACTGGAAACACGAGCCATTGGCCAAGATGCTACTGGGTATCATCCCGGACTGTTCTTAACCTCAGCTCAGCGGGAAGCTGTGGAAGCTCTCATCCAGGAACTTCCAAAGTTTAGGCTGAAGGCTGTCCCAACTGATTGCAGTGAATGCCCTATTTGCCTTGAAGAGTTTCATGTAGGGAATGAGGTTCGTGGTATGCCATGTGCTCACAATTTCCACGTTGAATGTATTGACGAATGGCTTCGTCTGAATGTCAAATGTCCTCGATGCCGCTCCTCCGTCTTCCCAAATCTCGACCTCAGCGCTCTTTCTAACCTGCATTCAGACACAGAACAAACATCTCACAATGCTTTTGTAACAACCCAACCTTCAAGTCTATCGAGATCAACTCCAACTCACGCAGCTCATACCGAAAACAGTCTCCAAACTGATACTGCCTTAGATGCTTTGGAAAATGGAAACTCACCCATGCCATCTGCTTCTCCTCATTGA

Protein sequence

MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFIDNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEEGQKWGFLIWLLFSYCALLCIACMSVGKWLVRRQAHLFRAQQGIPVSEYGVLVDMIRVPDWAYEAAGLETRAIGQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLEEFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSHNAFVTTQPSSLSRSTPTHAAHTENSLQTDTALDALENGNSPMPSASPH*

Homology

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BLAST of CSPI01G08440 vs. ExPASy Swiss-Prot
Match: Q8GYT9 (E3 ubiquitin-protein ligase SIS3 OS=Arabidopsis thaliana OX=3702 GN=SIS3 PE=2 SV=2)

HSP 1 Score: 523.9 bits (1348), Expect = 1.4e-147
Identity = 254/353 (71.95%), Postives = 283/353 (80.17%), Query Frame = 0


Query: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60
           MA+RGVDFKWYDGFFLSMLATS+IIV +NW RY  C YPLHIWIVVDYT+VF FR+ +F+
Sbjct: 1   MAMRGVDFKWYDGFFLSMLATSVIIVAVNWNRYRACEYPLHIWIVVDYTTVFIFRVFMFV 60

Query: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120
           DN L++G+GLDFG QQR A FCGRVVVLS+LSLLLYPFL AWTVIGT WF  +K CLPEE
Sbjct: 61  DNGLASGLGLDFGSQQRNAMFCGRVVVLSVLSLLLYPFLWAWTVIGTQWFTKSKTCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLVRRQAHLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWL+FSYC LLCIA + VGKWL RRQ HL RAQQGIP+SE+G+LVDMIRVPDW
Sbjct: 121 GQKWGFLIWLMFSYCGLLCIAFICVGKWLTRRQVHLLRAQQGIPISEFGILVDMIRVPDW 180

Query: 181 AYEAAGLETRAIGQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240
           A+EAAG E R I QDA  YHPGL+LT AQ EAVEALIQELPKFRLKAVP DC EC ICLE
Sbjct: 181 AFEAAGQEMRGISQDAATYHPGLYLTPAQTEAVEALIQELPKFRLKAVPDDCGECLICLE 240

Query: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSD-TEQ-- 300
           EFH+G+EVRG+PCAHNFHVECID+WLRLNVKCPRCR SVFP+LDLSALSNL S  TEQ  
Sbjct: 241 EFHIGHEVRGLPCAHNFHVECIDQWLRLNVKCPRCRCSVFPDLDLSALSNLQSSGTEQHS 300

Query: 301 -----TSHNAFVTTQPSS----LSRSTPTHAAHTENSLQTDTALDALENGNSP 342
                TS   ++ +QP S    L   +  H  H      TDTAL+  ENG  P
Sbjct: 301 QVNTETSEARYIRSQPQSESYFLRVQSLIHPVH------TDTALETAENGGVP 347

[Close]

BLAST of CSPI01G08440 vs. ExPASy Swiss-Prot
Match: Q9LN71 (E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g12760 PE=1 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 8.7e-12
Identity = 49/197 (24.87%), Postives = 83/197 (42.13%), Query Frame = 0


Query: 98  FLCAWTVIGTLWFRDAKDCLPEEGQK--W------GFLIWLLFSYCALLCIACMSVGKWL 157
           F   W +IG  W       L +E  +  W      GF ++ +    AL C+  ++V   L
Sbjct: 223 FSFIWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACVIGIAVCCCL 282

Query: 158 VRRQAHLFRAQQGIPVSEYGVLVDMIRVPDWAYEAAGLETRAIGQDATGYHPGLFLTSAQ 217
               A L+     +   E     D+ ++  + +   G   +    +A G   G+      
Sbjct: 283 PCIIAVLY----AVADQEGASKEDIEQLTKFKFRKLGDANKHTNDEAQGTTEGIMTECGT 342

Query: 218 REAVE-ALIQELPKFRLKAVPTDCSECPICLEEFHVGNEVRGMPCAHNFHVECIDEWLRL 277
              +E  L+QE             +EC ICL  +  G E+R +PC H+FH  C+D+WL +
Sbjct: 343 DSPIEHTLLQE------------DAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYI 402

Query: 278 NVKCPRCRSSVFPNLDL 286
           N  CP C+ ++  + +L
Sbjct: 403 NATCPLCKYNILKSSNL 403

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BLAST of CSPI01G08440 vs. ExPASy Swiss-Prot
Match: Q9FKX5 (NEP1-interacting protein-like 1 OS=Arabidopsis thaliana OX=3702 GN=ATL27 PE=2 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.5e-11
Identity = 29/78 (37.18%), Postives = 43/78 (55.13%), Query Frame = 0


Query: 200 HPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLEEFHVGNEVRGMP-CAHNFH 259
           H  +F T+  +      +  +PK R+     +   C +CL++F VG  VR +P C H FH
Sbjct: 141 HTDIFDTAISKGLTGDSLNRIPKVRITDTSPEIVSCSVCLQDFQVGETVRSLPHCHHMFH 200

Query: 260 VECIDEWLRLNVKCPRCR 277
           + CID+WLR +  CP CR
Sbjct: 201 LPCIDKWLRRHASCPLCR 218

[Close]

BLAST of CSPI01G08440 vs. ExPASy Swiss-Prot
Match: Q93Z92 (E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g11680 PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 7.4e-11
Identity = 34/107 (31.78%), Postives = 53/107 (49.53%), Query Frame = 0


Query: 203 LFLTSAQREAVEALIQELPKFRL-------------KAVPTDC--------------SEC 262
           L+  + Q  A +  I ++PKFR              + + T+C              +EC
Sbjct: 279 LYAVADQEGASKNDIDQMPKFRFTKTGNVEKLSGKARGIMTECGTDSPIERSLSPEDAEC 338

Query: 263 PICLEEFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPN 283
            ICL E+  G E+R +PC H+FH  CID+WL +N +CP C+ ++  N
Sbjct: 339 CICLCEYEDGVELRELPCNHHFHCTCIDKWLHINSRCPLCKFNILKN 385

[Close]

BLAST of CSPI01G08440 vs. ExPASy Swiss-Prot
Match: Q6DIP3 (E3 ubiquitin-protein ligase RNF126 OS=Xenopus tropicalis OX=8364 GN=rnf126 PE=2 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.4e-09
Identity = 52/171 (30.41%), Postives = 77/171 (45.03%), Query Frame = 0


Query: 159 AQQGIPVSEYGVLVDMIRVPDWAYEAAGLE---TRAIGQDATGYHPGLFLTSAQREAVEA 218
           A   + V  +GVL       D+A+ A GL+   T+ + Q         F  +    A   
Sbjct: 158 AMSNLGVGPWGVLHS--NPMDYAWGANGLDTIITQLLNQ---------FENTGPPPADTE 217

Query: 219 LIQELPKFRLKAVPTDCS-ECPICLEEFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPR 278
            IQ LP  ++         ECP+C E++ VG  VR +PC H FH +CI  WL  +  CP 
Sbjct: 218 KIQALPTIQITEEHVGSGLECPVCKEDYTVGESVRQLPCNHLFHNDCIIPWLEQHDTCPV 277

Query: 279 CRSSVFPNLDLSALSNLHSDTEQTSHNAFVTTQPSSLSRSTPTHAAHTENS 326
           CR S+      +  +N    TE T    F ++  SS S ++PT   +  N+
Sbjct: 278 CRKSLSGQ---NTATNPPGLTEMT----FSSSSTSSSSSTSPTDENNAANN 310

[Close]

BLAST of CSPI01G08440 vs. ExPASy TrEMBL
Match: A0A0A0LQW0 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G046030 PE=4 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 7.0e-206
Identity = 348/348 (100.00%), Postives = 348/348 (100.00%), Query Frame = 0


Query: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60
           MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI
Sbjct: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60

Query: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120
           DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE
Sbjct: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLVRRQAHLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALLCIACMSVGKWLVRRQAHLFRAQQGIPVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLVRRQAHLFRAQQGIPVSEYGVLVDMIRVPDW 180

Query: 181 AYEAAGLETRAIGQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240
           AYEAAGLETRAIGQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AYEAAGLETRAIGQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH 300
           EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH
Sbjct: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH 300

Query: 301 NAFVTTQPSSLSRSTPTHAAHTENSLQTDTALDALENGNSPMPSASPH 349
           NAFVTTQPSSLSRSTPTHAAHTENSLQTDTALDALENGNSPMPSASPH
Sbjct: 301 NAFVTTQPSSLSRSTPTHAAHTENSLQTDTALDALENGNSPMPSASPH 348

[Close]

BLAST of CSPI01G08440 vs. ExPASy TrEMBL
Match: A0A5D3BHC8 (E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G003600 PE=4 SV=1)

HSP 1 Score: 711.4 bits (1835), Expect = 1.8e-201
Identity = 343/350 (98.00%), Postives = 344/350 (98.29%), Query Frame = 0


Query: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60
           MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI
Sbjct: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60

Query: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120
           DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE
Sbjct: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLVRRQAHLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALLCIACMSVGKWL RRQAHLFRAQQG+PVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLARRQAHLFRAQQGLPVSEYGVLVDMIRVPDW 180

Query: 181 AYEAAGLETRAIGQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240
           AYEAAGLETRAI QDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AYEAAGLETRAIAQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH 300
           EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH
Sbjct: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH 300

Query: 301 NAFVTTQPSSLSRSTPTHAAHTEN--SLQTDTALDALENGNSPMPSASPH 349
           NAFVTTQPSSLSRSTPTHAAHTEN  SL TDTALDALENGNS MPSASPH
Sbjct: 301 NAFVTTQPSSLSRSTPTHAAHTENTASLHTDTALDALENGNSSMPSASPH 350

[Close]

BLAST of CSPI01G08440 vs. ExPASy TrEMBL
Match: A0A1S3AVR9 (E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483452 PE=4 SV=1)

HSP 1 Score: 711.4 bits (1835), Expect = 1.8e-201
Identity = 343/350 (98.00%), Postives = 344/350 (98.29%), Query Frame = 0


Query: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60
           MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI
Sbjct: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60

Query: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120
           DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE
Sbjct: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLVRRQAHLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALLCIACMSVGKWL RRQAHLFRAQQG+PVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLARRQAHLFRAQQGLPVSEYGVLVDMIRVPDW 180

Query: 181 AYEAAGLETRAIGQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240
           AYEAAGLETRAI QDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AYEAAGLETRAIAQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH 300
           EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH
Sbjct: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH 300

Query: 301 NAFVTTQPSSLSRSTPTHAAHTEN--SLQTDTALDALENGNSPMPSASPH 349
           NAFVTTQPSSLSRSTPTHAAHTEN  SL TDTALDALENGNS MPSASPH
Sbjct: 301 NAFVTTQPSSLSRSTPTHAAHTENTASLHTDTALDALENGNSSMPSASPH 350

[Close]

BLAST of CSPI01G08440 vs. ExPASy TrEMBL
Match: A0A5A7UGY5 (E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G00650 PE=4 SV=1)

HSP 1 Score: 709.5 bits (1830), Expect = 6.8e-201
Identity = 342/350 (97.71%), Postives = 343/350 (98.00%), Query Frame = 0


Query: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60
           MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI
Sbjct: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60

Query: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120
           DNALSAGMGLDFGWQQRYA FCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE
Sbjct: 61  DNALSAGMGLDFGWQQRYAHFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLVRRQAHLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALLCIACMSVGKWL RRQAHLFRAQQG+PVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLARRQAHLFRAQQGLPVSEYGVLVDMIRVPDW 180

Query: 181 AYEAAGLETRAIGQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240
           AYEAAGLETRAI QDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AYEAAGLETRAIAQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH 300
           EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH
Sbjct: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH 300

Query: 301 NAFVTTQPSSLSRSTPTHAAHTEN--SLQTDTALDALENGNSPMPSASPH 349
           NAFVTTQPSSLSRSTPTHAAHTEN  SL TDTALDALENGNS MPSASPH
Sbjct: 301 NAFVTTQPSSLSRSTPTHAAHTENTASLHTDTALDALENGNSSMPSASPH 350

[Close]

BLAST of CSPI01G08440 vs. ExPASy TrEMBL
Match: A0A6J1KIZ6 (E3 ubiquitin-protein ligase SIS3-like OS=Cucurbita maxima OX=3661 GN=LOC111495030 PE=4 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 1.6e-189
Identity = 322/345 (93.33%), Postives = 335/345 (97.10%), Query Frame = 0


Query: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60
           MAIRGVDFKWYDGFFLSMLATSIIIV+INWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI
Sbjct: 1   MAIRGVDFKWYDGFFLSMLATSIIIVSINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60

Query: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120
           DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE
Sbjct: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLVRRQAHLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALLC+AC+S+GKWL RRQAHLFRAQQGIPVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALLCVACLSIGKWLARRQAHLFRAQQGIPVSEYGVLVDMIRVPDW 180

Query: 181 AYEAAGLETRAIGQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240
           A+EAAGLETR IGQDATGYHPGLFLTSAQREAVEALIQ+LPKFRLKAVPTDCSECPICLE
Sbjct: 181 AFEAAGLETRRIGQDATGYHPGLFLTSAQREAVEALIQDLPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH 300
           EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTS 
Sbjct: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSA 300

Query: 301 NAFVTTQPSSLSRSTPTH-AAHTENS--LQTDTALDALENGNSPM 343
            AFVT+QPSSLSRS P+H A+HTEN+  L TDT LDA+ENG+SP+
Sbjct: 301 IAFVTSQPSSLSRSAPSHRASHTENTAGLYTDTTLDAMENGSSPI 345

[Close]

BLAST of CSPI01G08440 vs. NCBI nr
Match: XP_004152472.1 (E3 ubiquitin-protein ligase SIS3 isoform X1 [Cucumis sativus] >KGN64305.1 hypothetical protein Csa_013147 [Cucumis sativus])

HSP 1 Score: 726.1 bits (1873), Expect = 1.4e-205
Identity = 348/348 (100.00%), Postives = 348/348 (100.00%), Query Frame = 0


Query: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60
           MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI
Sbjct: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60

Query: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120
           DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE
Sbjct: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLVRRQAHLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALLCIACMSVGKWLVRRQAHLFRAQQGIPVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLVRRQAHLFRAQQGIPVSEYGVLVDMIRVPDW 180

Query: 181 AYEAAGLETRAIGQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240
           AYEAAGLETRAIGQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AYEAAGLETRAIGQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH 300
           EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH
Sbjct: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH 300

Query: 301 NAFVTTQPSSLSRSTPTHAAHTENSLQTDTALDALENGNSPMPSASPH 349
           NAFVTTQPSSLSRSTPTHAAHTENSLQTDTALDALENGNSPMPSASPH
Sbjct: 301 NAFVTTQPSSLSRSTPTHAAHTENSLQTDTALDALENGNSPMPSASPH 348

[Close]

BLAST of CSPI01G08440 vs. NCBI nr
Match: XP_008438310.1 (PREDICTED: E3 ubiquitin-protein ligase SIS3-like isoform X1 [Cucumis melo] >TYJ99152.1 E3 ubiquitin-protein ligase SIS3-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 711.4 bits (1835), Expect = 3.7e-201
Identity = 343/350 (98.00%), Postives = 344/350 (98.29%), Query Frame = 0


Query: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60
           MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI
Sbjct: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60

Query: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120
           DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE
Sbjct: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLVRRQAHLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALLCIACMSVGKWL RRQAHLFRAQQG+PVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLARRQAHLFRAQQGLPVSEYGVLVDMIRVPDW 180

Query: 181 AYEAAGLETRAIGQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240
           AYEAAGLETRAI QDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AYEAAGLETRAIAQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH 300
           EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH
Sbjct: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH 300

Query: 301 NAFVTTQPSSLSRSTPTHAAHTEN--SLQTDTALDALENGNSPMPSASPH 349
           NAFVTTQPSSLSRSTPTHAAHTEN  SL TDTALDALENGNS MPSASPH
Sbjct: 301 NAFVTTQPSSLSRSTPTHAAHTENTASLHTDTALDALENGNSSMPSASPH 350

[Close]

BLAST of CSPI01G08440 vs. NCBI nr
Match: KAA0055223.1 (E3 ubiquitin-protein ligase SIS3-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 709.5 bits (1830), Expect = 1.4e-200
Identity = 342/350 (97.71%), Postives = 343/350 (98.00%), Query Frame = 0


Query: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60
           MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI
Sbjct: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60

Query: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120
           DNALSAGMGLDFGWQQRYA FCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE
Sbjct: 61  DNALSAGMGLDFGWQQRYAHFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLVRRQAHLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALLCIACMSVGKWL RRQAHLFRAQQG+PVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLARRQAHLFRAQQGLPVSEYGVLVDMIRVPDW 180

Query: 181 AYEAAGLETRAIGQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240
           AYEAAGLETRAI QDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AYEAAGLETRAIAQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH 300
           EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH
Sbjct: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH 300

Query: 301 NAFVTTQPSSLSRSTPTHAAHTEN--SLQTDTALDALENGNSPMPSASPH 349
           NAFVTTQPSSLSRSTPTHAAHTEN  SL TDTALDALENGNS MPSASPH
Sbjct: 301 NAFVTTQPSSLSRSTPTHAAHTENTASLHTDTALDALENGNSSMPSASPH 350

[Close]

BLAST of CSPI01G08440 vs. NCBI nr
Match: XP_038895423.1 (E3 ubiquitin-protein ligase SIS3-like isoform X1 [Benincasa hispida])

HSP 1 Score: 697.2 bits (1798), Expect = 7.2e-197
Identity = 335/350 (95.71%), Postives = 340/350 (97.14%), Query Frame = 0


Query: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60
           MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI
Sbjct: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60

Query: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120
           DNALSAGMGLDFGWQQRYARFCGRVVVLS+LSLLLYPFLCAWTVIGTLWFRDAKDCLPEE
Sbjct: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSVLSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLVRRQAHLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALLCIACMSVGKWL RRQAHLFRAQQG+PVSEYGVL+DMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLARRQAHLFRAQQGMPVSEYGVLIDMIRVPDW 180

Query: 181 AYEAAGLETRAIGQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240
           AYEAAGLE R IGQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AYEAAGLEIRGIGQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH 300
           EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTS 
Sbjct: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSD 300

Query: 301 NAFVTTQPSSLSRSTPTHAAHTENS--LQTDTALDALENGNSPMPSASPH 349
           NAFVTTQPSSL RSTPT AAHTEN+  L TDTALDALENGNS +PSASPH
Sbjct: 301 NAFVTTQPSSLLRSTPTRAAHTENTAGLYTDTALDALENGNSSLPSASPH 350

[Close]

BLAST of CSPI01G08440 vs. NCBI nr
Match: XP_023000660.1 (E3 ubiquitin-protein ligase SIS3-like [Cucurbita maxima])

HSP 1 Score: 671.8 bits (1732), Expect = 3.2e-189
Identity = 322/345 (93.33%), Postives = 335/345 (97.10%), Query Frame = 0


Query: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60
           MAIRGVDFKWYDGFFLSMLATSIIIV+INWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI
Sbjct: 1   MAIRGVDFKWYDGFFLSMLATSIIIVSINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60

Query: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120
           DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE
Sbjct: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLVRRQAHLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALLC+AC+S+GKWL RRQAHLFRAQQGIPVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALLCVACLSIGKWLARRQAHLFRAQQGIPVSEYGVLVDMIRVPDW 180

Query: 181 AYEAAGLETRAIGQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240
           A+EAAGLETR IGQDATGYHPGLFLTSAQREAVEALIQ+LPKFRLKAVPTDCSECPICLE
Sbjct: 181 AFEAAGLETRRIGQDATGYHPGLFLTSAQREAVEALIQDLPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSH 300
           EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTS 
Sbjct: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSDTEQTSA 300

Query: 301 NAFVTTQPSSLSRSTPTH-AAHTENS--LQTDTALDALENGNSPM 343
            AFVT+QPSSLSRS P+H A+HTEN+  L TDT LDA+ENG+SP+
Sbjct: 301 IAFVTSQPSSLSRSAPSHRASHTENTAGLYTDTTLDAMENGSSPI 345

[Close]

BLAST of CSPI01G08440 vs. TAIR 10
Match: AT3G47990.1 (SUGAR-INSENSITIVE 3 )

HSP 1 Score: 523.9 bits (1348), Expect = 1.0e-148
Identity = 254/353 (71.95%), Postives = 283/353 (80.17%), Query Frame = 0


Query: 1   MAIRGVDFKWYDGFFLSMLATSIIIVTINWRRYNTCTYPLHIWIVVDYTSVFAFRLLLFI 60
           MA+RGVDFKWYDGFFLSMLATS+IIV +NW RY  C YPLHIWIVVDYT+VF FR+ +F+
Sbjct: 1   MAMRGVDFKWYDGFFLSMLATSVIIVAVNWNRYRACEYPLHIWIVVDYTTVFIFRVFMFV 60

Query: 61  DNALSAGMGLDFGWQQRYARFCGRVVVLSILSLLLYPFLCAWTVIGTLWFRDAKDCLPEE 120
           DN L++G+GLDFG QQR A FCGRVVVLS+LSLLLYPFL AWTVIGT WF  +K CLPEE
Sbjct: 61  DNGLASGLGLDFGSQQRNAMFCGRVVVLSVLSLLLYPFLWAWTVIGTQWFTKSKTCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALLCIACMSVGKWLVRRQAHLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWL+FSYC LLCIA + VGKWL RRQ HL RAQQGIP+SE+G+LVDMIRVPDW
Sbjct: 121 GQKWGFLIWLMFSYCGLLCIAFICVGKWLTRRQVHLLRAQQGIPISEFGILVDMIRVPDW 180

Query: 181 AYEAAGLETRAIGQDATGYHPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLE 240
           A+EAAG E R I QDA  YHPGL+LT AQ EAVEALIQELPKFRLKAVP DC EC ICLE
Sbjct: 181 AFEAAGQEMRGISQDAATYHPGLYLTPAQTEAVEALIQELPKFRLKAVPDDCGECLICLE 240

Query: 241 EFHVGNEVRGMPCAHNFHVECIDEWLRLNVKCPRCRSSVFPNLDLSALSNLHSD-TEQ-- 300
           EFH+G+EVRG+PCAHNFHVECID+WLRLNVKCPRCR SVFP+LDLSALSNL S  TEQ  
Sbjct: 241 EFHIGHEVRGLPCAHNFHVECIDQWLRLNVKCPRCRCSVFPDLDLSALSNLQSSGTEQHS 300

Query: 301 -----TSHNAFVTTQPSS----LSRSTPTHAAHTENSLQTDTALDALENGNSP 342
                TS   ++ +QP S    L   +  H  H      TDTAL+  ENG  P
Sbjct: 301 QVNTETSEARYIRSQPQSESYFLRVQSLIHPVH------TDTALETAENGGVP 347

[Close]

BLAST of CSPI01G08440 vs. TAIR 10
Match: AT1G12760.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 72.8 bits (177), Expect = 6.2e-13
Identity = 49/197 (24.87%), Postives = 83/197 (42.13%), Query Frame = 0


Query: 98  FLCAWTVIGTLWFRDAKDCLPEEGQK--W------GFLIWLLFSYCALLCIACMSVGKWL 157
           F   W +IG  W       L +E  +  W      GF ++ +    AL C+  ++V   L
Sbjct: 223 FSFIWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACVIGIAVCCCL 282

Query: 158 VRRQAHLFRAQQGIPVSEYGVLVDMIRVPDWAYEAAGLETRAIGQDATGYHPGLFLTSAQ 217
               A L+     +   E     D+ ++  + +   G   +    +A G   G+      
Sbjct: 283 PCIIAVLY----AVADQEGASKEDIEQLTKFKFRKLGDANKHTNDEAQGTTEGIMTECGT 342

Query: 218 REAVE-ALIQELPKFRLKAVPTDCSECPICLEEFHVGNEVRGMPCAHNFHVECIDEWLRL 277
              +E  L+QE             +EC ICL  +  G E+R +PC H+FH  C+D+WL +
Sbjct: 343 DSPIEHTLLQE------------DAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYI 402

Query: 278 NVKCPRCRSSVFPNLDL 286
           N  CP C+ ++  + +L
Sbjct: 403 NATCPLCKYNILKSSNL 403

[Close]

BLAST of CSPI01G08440 vs. TAIR 10
Match: AT1G12760.2 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 72.8 bits (177), Expect = 6.2e-13
Identity = 49/197 (24.87%), Postives = 83/197 (42.13%), Query Frame = 0


Query: 98  FLCAWTVIGTLWFRDAKDCLPEEGQK--W------GFLIWLLFSYCALLCIACMSVGKWL 157
           F   W +IG  W       L +E  +  W      GF ++ +    AL C+  ++V   L
Sbjct: 152 FSFIWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACVIGIAVCCCL 211

Query: 158 VRRQAHLFRAQQGIPVSEYGVLVDMIRVPDWAYEAAGLETRAIGQDATGYHPGLFLTSAQ 217
               A L+     +   E     D+ ++  + +   G   +    +A G   G+      
Sbjct: 212 PCIIAVLY----AVADQEGASKEDIEQLTKFKFRKLGDANKHTNDEAQGTTEGIMTECGT 271

Query: 218 REAVE-ALIQELPKFRLKAVPTDCSECPICLEEFHVGNEVRGMPCAHNFHVECIDEWLRL 277
              +E  L+QE             +EC ICL  +  G E+R +PC H+FH  C+D+WL +
Sbjct: 272 DSPIEHTLLQE------------DAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYI 331

Query: 278 NVKCPRCRSSVFPNLDL 286
           N  CP C+ ++  + +L
Sbjct: 332 NATCPLCKYNILKSSNL 332

[Close]

BLAST of CSPI01G08440 vs. TAIR 10
Match: AT5G66070.1 (RING/U-box superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 1.1e-12
Identity = 29/78 (37.18%), Postives = 43/78 (55.13%), Query Frame = 0


Query: 200 HPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLEEFHVGNEVRGMP-CAHNFH 259
           H  +F T+  +      +  +PK R+     +   C +CL++F VG  VR +P C H FH
Sbjct: 141 HTDIFDTAISKGLTGDSLNRIPKVRITDTSPEIVSCSVCLQDFQVGETVRSLPHCHHMFH 200

Query: 260 VECIDEWLRLNVKCPRCR 277
           + CID+WLR +  CP CR
Sbjct: 201 LPCIDKWLRRHASCPLCR 218

[Close]

BLAST of CSPI01G08440 vs. TAIR 10
Match: AT5G66070.2 (RING/U-box superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 1.1e-12
Identity = 29/78 (37.18%), Postives = 43/78 (55.13%), Query Frame = 0


Query: 200 HPGLFLTSAQREAVEALIQELPKFRLKAVPTDCSECPICLEEFHVGNEVRGMP-CAHNFH 259
           H  +F T+  +      +  +PK R+     +   C +CL++F VG  VR +P C H FH
Sbjct: 165 HTDIFDTAISKGLTGDSLNRIPKVRITDTSPEIVSCSVCLQDFQVGETVRSLPHCHHMFH 224

Query: 260 VECIDEWLRLNVKCPRCR 277
           + CID+WLR +  CP CR
Sbjct: 225 LPCIDKWLRRHASCPLCR 242

The following BLAST results are available for this feature:

BLAST of CSPI01G08440 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GYT9	1.4e-147	71.95	E3 ubiquitin-protein ligase SIS3 OS=Arabidopsis thaliana OX=3702 GN=SIS3 PE=2 SV...	[more]
Q9LN71	8.7e-12	24.87	E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g127...	[more]
Q9FKX5	1.5e-11	37.18	NEP1-interacting protein-like 1 OS=Arabidopsis thaliana OX=3702 GN=ATL27 PE=2 SV...	[more]
Q93Z92	7.4e-11	31.78	E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g116...	[more]
Q6DIP3	1.4e-09	30.41	E3 ubiquitin-protein ligase RNF126 OS=Xenopus tropicalis OX=8364 GN=rnf126 PE=2 ...	[more]

BLAST of CSPI01G08440 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LQW0	7.0e-206	100.00	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G046030 P...	[more]
A0A5D3BHC8	1.8e-201	98.00	E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3AVR9	1.8e-201	98.00	E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A5A7UGY5	6.8e-201	97.71	E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Cucumis melo var. makuwa OX=...	[more]
A0A6J1KIZ6	1.6e-189	93.33	E3 ubiquitin-protein ligase SIS3-like OS=Cucurbita maxima OX=3661 GN=LOC11149503...	[more]

BLAST of CSPI01G08440 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152472.1	1.4e-205	100.00	E3 ubiquitin-protein ligase SIS3 isoform X1 [Cucumis sativus] >KGN64305.1 hypoth...	[more]
XP_008438310.1	3.7e-201	98.00	PREDICTED: E3 ubiquitin-protein ligase SIS3-like isoform X1 [Cucumis melo] >TYJ9...	[more]
KAA0055223.1	1.4e-200	97.71	E3 ubiquitin-protein ligase SIS3-like isoform X1 [Cucumis melo var. makuwa]	[more]
XP_038895423.1	7.2e-197	95.71	E3 ubiquitin-protein ligase SIS3-like isoform X1 [Benincasa hispida]	[more]
XP_023000660.1	3.2e-189	93.33	E3 ubiquitin-protein ligase SIS3-like [Cucurbita maxima]	[more]

BLAST of CSPI01G08440 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G47990.1	1.0e-148	71.95	SUGAR-INSENSITIVE 3	[more]
AT1G12760.1	6.2e-13	24.87	Zinc finger, C3HC4 type (RING finger) family protein	[more]
AT1G12760.2	6.2e-13	24.87	Zinc finger, C3HC4 type (RING finger) family protein	[more]
AT5G66070.1	1.1e-12	37.18	RING/U-box superfamily protein	[more]
AT5G66070.2	1.1e-12	37.18	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 235..275 e-value: 1.1E-6 score: 38.2
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 234..276 e-value: 1.7E-13 score: 50.7
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 235..276 score: 12.769585
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 204..281 e-value: 8.4E-20 score: 72.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 297..324
None	No IPR available	PANTHER	PTHR47179:SF1	E3 UBIQUITIN-PROTEIN LIGASE SIS3	coord: 1..330
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 216..284
IPR044793	E3 ubiquitin-protein ligase SIS3	PANTHER	PTHR47179	E3 UBIQUITIN-PROTEIN LIGASE SIS3	coord: 1..330

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G08440.1	CSPI01G08440.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0010182	sugar mediated signaling pathway
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004842	ubiquitin-protein transferase activity