CSPI01G08370 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G08370
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	spermidine synthase 1-like
Location	Chr1: 5264743 .. 5265005 (-)
RNA-Seq Expression	CSPI01G08370
Synteny	CSPI01G08370

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTCTGTTCAACTGCCGGTCCATCAGTCGACTTCAAACGCCCGGTGAATCCGCTGGATCCAAGTAATTTTGGTGTTGCGCAAGGACCCCCCAAGTTCTACAACTCAGAGGTGATTTCTTCTTACTTTTTGCTCGCAATTGTTCATAGGCCCCTTATAAGATCCAACTAATTTGGCTGTTCAATTCTGCTGTTTAGATTCATACTGCTGCGTTTAGTCTTCCTGCGTTCGCAAAGTCGGCAATGGGTTCCAAATACGAATGA

mRNA sequence

ATGCTCTGTTCAACTGCCGGTCCATCAGTCGACTTCAAACGCCCGGTGAATCCGCTGGATCCAAGTAATTTTGGTGTTGCGCAAGGACCCCCCAAGTTCTACAACTCAGAGATTCATACTGCTGCGTTTAGTCTTCCTGCGTTCGCAAAGTCGGCAATGGGTTCCAAATACGAATGA

Coding sequence (CDS)

ATGCTCTGTTCAACTGCCGGTCCATCAGTCGACTTCAAACGCCCGGTGAATCCGCTGGATCCAAGTAATTTTGGTGTTGCGCAAGGACCCCCCAAGTTCTACAACTCAGAGATTCATACTGCTGCGTTTAGTCTTCCTGCGTTCGCAAAGTCGGCAATGGGTTCCAAATACGAATGA

Protein sequence

MLCSTAGPSVDFKRPVNPLDPSNFGVAQGPPKFYNSEIHTAAFSLPAFAKSAMGSKYE*

Homology

[Close]

BLAST of CSPI01G08370 vs. ExPASy Swiss-Prot
Match: Q9ZTR1 (Spermidine synthase 1 OS=Pisum sativum OX=3888 GN=SPDSYN1 PE=2 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 2.7e-14
Identity = 37/56 (66.07%), Postives = 43/56 (76.79%), Query Frame = 0


Query: 1   MLCSTAGPSVDFKRPVNPLDPSNFGVAQGPPKFYNSEIHTAAFSLPAFAKSAMGSK 57
           MLCST GPSVDFK PVNP+D ++   A  P KFYN EIH+AAF LP+FAK A+ SK
Sbjct: 277 MLCSTEGPSVDFKHPVNPIDENDSQQAARPLKFYNREIHSAAFCLPSFAKRAIASK 332

[Close]

BLAST of CSPI01G08370 vs. ExPASy Swiss-Prot
Match: Q9ZTR0 (Spermidine synthase 2 OS=Pisum sativum OX=3888 GN=SPDSYN2 PE=2 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 3.5e-14
Identity = 37/56 (66.07%), Postives = 42/56 (75.00%), Query Frame = 0


Query: 1   MLCSTAGPSVDFKRPVNPLDPSNFGVAQGPPKFYNSEIHTAAFSLPAFAKSAMGSK 57
           MLCST GP VDFK PVNP+D  +   +  P KFYNSEIHTAAF LP+FAK  +GSK
Sbjct: 285 MLCSTEGPLVDFKHPVNPIDQKDCQKSVRPLKFYNSEIHTAAFCLPSFAKRKIGSK 340

[Close]

BLAST of CSPI01G08370 vs. ExPASy Swiss-Prot
Match: Q96557 (Spermidine synthase 2 OS=Datura stramonium OX=4076 PE=2 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 6.0e-14
Identity = 34/56 (60.71%), Postives = 42/56 (75.00%), Query Frame = 0


Query: 1   MLCSTAGPSVDFKRPVNPLDPSNFGVAQGPPKFYNSEIHTAAFSLPAFAKSAMGSK 57
           MLCST GP+VDFK P+NP+D  + G   GP KFYNSEIH A+F LP+FAK  + +K
Sbjct: 260 MLCSTEGPAVDFKNPINPIDDESHGQTIGPLKFYNSEIHQASFCLPSFAKRVIETK 315

[Close]

BLAST of CSPI01G08370 vs. ExPASy Swiss-Prot
Match: Q9ZUB3 (Spermidine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=SPDSYN1 PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 3.0e-13
Identity = 35/56 (62.50%), Postives = 43/56 (76.79%), Query Frame = 0


Query: 1   MLCSTAGPSVDFKRPVNPLDPSNFGVAQGPPKFYNSEIHTAAFSLPAFAKSAMGSK 57
           MLCST GP VDFK P+NP+D S+   + GP KFYN+EIH+AAF LP+FAK  + SK
Sbjct: 278 MLCSTEGPDVDFKHPLNPIDESS-SKSNGPLKFYNAEIHSAAFCLPSFAKKVIESK 332

[Close]

BLAST of CSPI01G08370 vs. ExPASy Swiss-Prot
Match: Q96556 (Spermidine synthase 1 OS=Datura stramonium OX=4076 PE=2 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.1e-12
Identity = 34/57 (59.65%), Postives = 43/57 (75.44%), Query Frame = 0


Query: 1   MLCSTAGPSVDFKRPVNPLDPSNFGV-AQGPPKFYNSEIHTAAFSLPAFAKSAMGSK 57
           MLCST GP+VDFK P+NP+D  +     +GP KFYNSEIH+A+F LP+FAK  + SK
Sbjct: 250 MLCSTEGPAVDFKNPINPVDADDSHTKTRGPLKFYNSEIHSASFCLPSFAKRVIESK 306

[Close]

BLAST of CSPI01G08370 vs. ExPASy TrEMBL
Match: A0A0A0LTG5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G045970 PE=4 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.6e-25
Identity = 58/58 (100.00%), Postives = 58/58 (100.00%), Query Frame = 0


Query: 1  MLCSTAGPSVDFKRPVNPLDPSNFGVAQGPPKFYNSEIHTAAFSLPAFAKSAMGSKYE 59
          MLCSTAGPSVDFKRPVNPLDPSNFGVAQGPPKFYNSEIHTAAFSLPAFAKSAMGSKYE
Sbjct: 1  MLCSTAGPSVDFKRPVNPLDPSNFGVAQGPPKFYNSEIHTAAFSLPAFAKSAMGSKYE 58

[Close]

BLAST of CSPI01G08370 vs. ExPASy TrEMBL
Match: A0A1S3BF22 (spermidine synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103488909 PE=3 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 3.3e-23
Identity = 54/57 (94.74%), Postives = 56/57 (98.25%), Query Frame = 0


Query: 1   MLCSTAGPSVDFKRPVNPLDPSNFGVAQGPPKFYNSEIHTAAFSLPAFAKSAMGSKY 58
           MLCST GPSVDFKRPVNPLDPSNFGVA+GPPKFYNSEIH+AAFSLPAFAKSAMGSKY
Sbjct: 276 MLCSTDGPSVDFKRPVNPLDPSNFGVAKGPPKFYNSEIHSAAFSLPAFAKSAMGSKY 332

[Close]

BLAST of CSPI01G08370 vs. ExPASy TrEMBL
Match: A0A6J1C7X6 (spermidine synthase 1-like OS=Momordica charantia OX=3673 GN=LOC111009053 PE=3 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 3.8e-19
Identity = 48/57 (84.21%), Postives = 50/57 (87.72%), Query Frame = 0


Query: 1   MLCSTAGPSVDFKRPVNPLDPSNFGVAQGPPKFYNSEIHTAAFSLPAFAKSAMGSKY 58
           MLC+T GPSVDFK PVNPLDP NFGVA+ PP FYNSEIHTAAF LPAFAK AMGSKY
Sbjct: 280 MLCATDGPSVDFKHPVNPLDPINFGVAKRPPMFYNSEIHTAAFCLPAFAKLAMGSKY 336

[Close]

BLAST of CSPI01G08370 vs. ExPASy TrEMBL
Match: A0A5A7UJN5 (Spermidine synthase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G00590 PE=3 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 2.4e-18
Identity = 46/48 (95.83%), Postives = 48/48 (100.00%), Query Frame = 0


Query: 10  VDFKRPVNPLDPSNFGVAQGPPKFYNSEIHTAAFSLPAFAKSAMGSKY 58
           +DFKRPVNPLDPSNFGVA+GPPKFYNSEIHTAAFSLPAFAKSAMGSKY
Sbjct: 259 LDFKRPVNPLDPSNFGVAKGPPKFYNSEIHTAAFSLPAFAKSAMGSKY 306

[Close]

BLAST of CSPI01G08370 vs. ExPASy TrEMBL
Match: A0A2C9VPK0 (PABS domain-containing protein OS=Manihot esculenta OX=3983 GN=MANES_06G103200 PE=3 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 5.4e-18
Identity = 43/56 (76.79%), Postives = 49/56 (87.50%), Query Frame = 0


Query: 1   MLCSTAGPSVDFKRPVNPLDPSNFGVAQGPPKFYNSEIHTAAFSLPAFAKSAMGSK 57
           MLCST GP VDFK PVNPL+P N+GVA+GPP+FYNSE+HTAAFSLP+FAK   GSK
Sbjct: 288 MLCSTEGPPVDFKHPVNPLNPENYGVAKGPPRFYNSEVHTAAFSLPSFAKKMFGSK 343

[Close]

BLAST of CSPI01G08370 vs. NCBI nr
Match: KGN64299.1 (hypothetical protein Csa_013337 [Cucumis sativus])

HSP 1 Score: 124.4 bits (311), Expect = 3.3e-25
Identity = 58/58 (100.00%), Postives = 58/58 (100.00%), Query Frame = 0


Query: 1  MLCSTAGPSVDFKRPVNPLDPSNFGVAQGPPKFYNSEIHTAAFSLPAFAKSAMGSKYE 59
          MLCSTAGPSVDFKRPVNPLDPSNFGVAQGPPKFYNSEIHTAAFSLPAFAKSAMGSKYE
Sbjct: 1  MLCSTAGPSVDFKRPVNPLDPSNFGVAQGPPKFYNSEIHTAAFSLPAFAKSAMGSKYE 58

[Close]

BLAST of CSPI01G08370 vs. NCBI nr
Match: XP_031737481.1 (spermidine synthase 1-like [Cucumis sativus])

HSP 1 Score: 124.4 bits (311), Expect = 3.3e-25
Identity = 58/58 (100.00%), Postives = 58/58 (100.00%), Query Frame = 0


Query: 1   MLCSTAGPSVDFKRPVNPLDPSNFGVAQGPPKFYNSEIHTAAFSLPAFAKSAMGSKYE 59
           MLCSTAGPSVDFKRPVNPLDPSNFGVAQGPPKFYNSEIHTAAFSLPAFAKSAMGSKYE
Sbjct: 74  MLCSTAGPSVDFKRPVNPLDPSNFGVAQGPPKFYNSEIHTAAFSLPAFAKSAMGSKYE 131

[Close]

BLAST of CSPI01G08370 vs. NCBI nr
Match: XP_008446082.2 (PREDICTED: spermidine synthase 1-like [Cucumis melo])

HSP 1 Score: 116.7 bits (291), Expect = 6.8e-23
Identity = 54/57 (94.74%), Postives = 56/57 (98.25%), Query Frame = 0


Query: 1   MLCSTAGPSVDFKRPVNPLDPSNFGVAQGPPKFYNSEIHTAAFSLPAFAKSAMGSKY 58
           MLCST GPSVDFKRPVNPLDPSNFGVA+GPPKFYNSEIH+AAFSLPAFAKSAMGSKY
Sbjct: 276 MLCSTDGPSVDFKRPVNPLDPSNFGVAKGPPKFYNSEIHSAAFSLPAFAKSAMGSKY 332

[Close]

BLAST of CSPI01G08370 vs. NCBI nr
Match: XP_038893662.1 (spermidine synthase 2-like [Benincasa hispida])

HSP 1 Score: 109.4 bits (272), Expect = 1.1e-20
Identity = 51/57 (89.47%), Postives = 52/57 (91.23%), Query Frame = 0


Query: 1   MLCSTAGPSVDFKRPVNPLDPSNFGVAQGPPKFYNSEIHTAAFSLPAFAKSAMGSKY 58
           MLCSTAGPSVDFK PVN LDP NFGVA+GPPKFYN EIHTAAF LPAFAKSAMGSKY
Sbjct: 293 MLCSTAGPSVDFKHPVNLLDPGNFGVAKGPPKFYNPEIHTAAFCLPAFAKSAMGSKY 349

[Close]

BLAST of CSPI01G08370 vs. NCBI nr
Match: XP_022137674.1 (spermidine synthase 1-like [Momordica charantia])

HSP 1 Score: 103.2 bits (256), Expect = 7.8e-19
Identity = 48/57 (84.21%), Postives = 50/57 (87.72%), Query Frame = 0


Query: 1   MLCSTAGPSVDFKRPVNPLDPSNFGVAQGPPKFYNSEIHTAAFSLPAFAKSAMGSKY 58
           MLC+T GPSVDFK PVNPLDP NFGVA+ PP FYNSEIHTAAF LPAFAK AMGSKY
Sbjct: 280 MLCATDGPSVDFKHPVNPLDPINFGVAKRPPMFYNSEIHTAAFCLPAFAKLAMGSKY 336

[Close]

BLAST of CSPI01G08370 vs. TAIR 10
Match: AT1G23820.1 (spermidine synthase 1 )

HSP 1 Score: 75.1 bits (183), Expect = 2.1e-14
Identity = 35/56 (62.50%), Postives = 43/56 (76.79%), Query Frame = 0


Query: 1   MLCSTAGPSVDFKRPVNPLDPSNFGVAQGPPKFYNSEIHTAAFSLPAFAKSAMGSK 57
           MLCST GP VDFK P+NP+D S+   + GP KFYN+EIH+AAF LP+FAK  + SK
Sbjct: 322 MLCSTEGPDVDFKHPLNPIDESS-SKSNGPLKFYNAEIHSAAFCLPSFAKKVIESK 376

[Close]

BLAST of CSPI01G08370 vs. TAIR 10
Match: AT1G70310.1 (spermidine synthase 2 )

HSP 1 Score: 64.7 bits (156), Expect = 2.9e-11
Identity = 30/57 (52.63%), Postives = 41/57 (71.93%), Query Frame = 0


Query: 1   MLCSTAGPSVDFKRPVNPLDPSNFGV-AQGPPKFYNSEIHTAAFSLPAFAKSAMGSK 57
           MLCS+ GP VDFK+PV+ +D     + +  P K+YN+EIH+AAF LP+FAK  + SK
Sbjct: 282 MLCSSEGPQVDFKKPVSLIDTDESSIKSHCPLKYYNAEIHSAAFCLPSFAKKVIDSK 338

[Close]

BLAST of CSPI01G08370 vs. TAIR 10
Match: AT5G53120.1 (spermidine synthase 3 )

HSP 1 Score: 58.2 bits (139), Expect = 2.7e-09
Identity = 26/56 (46.43%), Postives = 39/56 (69.64%), Query Frame = 0


Query: 1   MLCSTAGPSVDFKRPVNPLDPSNFGVA-QGPPKFYNSEIHTAAFSLPAFAKSAMGS 56
           +LCST GP+VDFK P+NP++  +  +  +   KFYNS++H AAF+LP F +  + S
Sbjct: 300 VLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFALPTFLRREVAS 355

[Close]

BLAST of CSPI01G08370 vs. TAIR 10
Match: AT5G53120.2 (spermidine synthase 3 )

HSP 1 Score: 58.2 bits (139), Expect = 2.7e-09
Identity = 26/56 (46.43%), Postives = 39/56 (69.64%), Query Frame = 0


Query: 1   MLCSTAGPSVDFKRPVNPLDPSNFGVA-QGPPKFYNSEIHTAAFSLPAFAKSAMGS 56
           +LCST GP+VDFK P+NP++  +  +  +   KFYNS++H AAF+LP F +  + S
Sbjct: 300 VLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFALPTFLRREVAS 355

[Close]

BLAST of CSPI01G08370 vs. TAIR 10
Match: AT5G53120.3 (spermidine synthase 3 )

HSP 1 Score: 58.2 bits (139), Expect = 2.7e-09
Identity = 26/56 (46.43%), Postives = 39/56 (69.64%), Query Frame = 0


Query: 1   MLCSTAGPSVDFKRPVNPLDPSNFGVA-QGPPKFYNSEIHTAAFSLPAFAKSAMGS 56
           +LCST GP+VDFK P+NP++  +  +  +   KFYNS++H AAF+LP F +  + S
Sbjct: 300 VLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFALPTFLRREVAS 355

The following BLAST results are available for this feature:

BLAST of CSPI01G08370 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZTR1	2.7e-14	66.07	Spermidine synthase 1 OS=Pisum sativum OX=3888 GN=SPDSYN1 PE=2 SV=1	[more]
Q9ZTR0	3.5e-14	66.07	Spermidine synthase 2 OS=Pisum sativum OX=3888 GN=SPDSYN2 PE=2 SV=1	[more]
Q96557	6.0e-14	60.71	Spermidine synthase 2 OS=Datura stramonium OX=4076 PE=2 SV=1	[more]
Q9ZUB3	3.0e-13	62.50	Spermidine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=SPDSYN1 PE=1 SV=1	[more]
Q96556	1.1e-12	59.65	Spermidine synthase 1 OS=Datura stramonium OX=4076 PE=2 SV=1	[more]

BLAST of CSPI01G08370 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LTG5	1.6e-25	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G045970 PE=4 SV=1	[more]
A0A1S3BF22	3.3e-23	94.74	spermidine synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103488909 PE=3 SV=1	[more]
A0A6J1C7X6	3.8e-19	84.21	spermidine synthase 1-like OS=Momordica charantia OX=3673 GN=LOC111009053 PE=3 S...	[more]
A0A5A7UJN5	2.4e-18	95.83	Spermidine synthase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff...	[more]
A0A2C9VPK0	5.4e-18	76.79	PABS domain-containing protein OS=Manihot esculenta OX=3983 GN=MANES_06G103200 P...	[more]

BLAST of CSPI01G08370 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN64299.1	3.3e-25	100.00	hypothetical protein Csa_013337 [Cucumis sativus]	[more]
XP_031737481.1	3.3e-25	100.00	spermidine synthase 1-like [Cucumis sativus]	[more]
XP_008446082.2	6.8e-23	94.74	PREDICTED: spermidine synthase 1-like [Cucumis melo]	[more]
XP_038893662.1	1.1e-20	89.47	spermidine synthase 2-like [Benincasa hispida]	[more]
XP_022137674.1	7.8e-19	84.21	spermidine synthase 1-like [Momordica charantia]	[more]

BLAST of CSPI01G08370 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G23820.1	2.1e-14	62.50	spermidine synthase 1	[more]
AT1G70310.1	2.9e-11	52.63	spermidine synthase 2	[more]
AT5G53120.1	2.7e-09	46.43	spermidine synthase 3	[more]
AT5G53120.2	2.7e-09	46.43	spermidine synthase 3	[more]
AT5G53120.3	2.7e-09	46.43	spermidine synthase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 1..58 e-value: 1.5E-8 score: 36.3
None	No IPR available	PANTHER	PTHR11558:SF28	SYNTHASE 1, PUTATIVE-RELATED	coord: 1..52
IPR001045	Spermidine/spermine synthases	PANTHER	PTHR11558	SPERMIDINE/SPERMINE SYNTHASE	coord: 1..52

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G08370.1	CSPI01G08370.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0006596	polyamine biosynthetic process
molecular_function	GO:0030750	putrescine N-methyltransferase activity
molecular_function	GO:0004766	spermidine synthase activity
molecular_function	GO:0003824	catalytic activity