CSPI01G07650 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G07650
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionCoiled-coil domain-containing protein 12
LocationChr1: 4857158 .. 4860204 (-)
RNA-Seq ExpressionCSPI01G07650
SyntenyCSPI01G07650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTTGATGGGCCTCTTACGATCCGGGCCTTATATATATATCTCATTCCAAAGAGAAATGCGTCAAAAAGGCTTCTGCGCAAGAAAGAAACACTCGTTTTTCTCCCTAACGCCATTGCTCGAAGCTTCCTGTCTACTTTGTCTGTAAGTATATTTCTTCTGCGGAAGTCCTTAATTGCGTATGTTGTGTTTTCTCATCTTCATGTTTCTTCTACCGCTGTCTGCGGAAGTCGTTAATTGCGTATGCATTTTTGAAATCATAGACAGAGTTATTTTCGTATCAGGTATTTTACGCACAAAGGCAATGGCTACTGAAGAAGACTCCGTGGAACAAGTTGCTGCAGCACGTAAAGAGAGATTGAAAGCTCTAAGAGCTGCTCAGGAATTGTTAAACAATTCAGATGAGAAGAATTCTGGAGGAGAGGATAAAGAGAATGGAGCTACTGAAGATTCTGATGAAACGTGAGTGTATTCTCATTTAAATATCTATTCTGTGATATCTCTCCAAATTCATGCTACTCTCTTTTATATCTCGTTTTGAATGTCCTCTTTATCTCTTTTATCTCTGTCTATGTGTTTATTGCCTGACCTATTTTCCTTCCTTTAGCGTTTAGCCATCCTTTTTATCTTTGTGTCTGTCATCTGACTTATTTTCTCTCTTTTATTGTACGGCGGGAGTCTATATTGAAATTACATACATAGCATAGTGAGCCAAGTTTCTAGAATCTGGCTTGAAACATGTTACAGGCTACTAACGCATGCTGTTGCCAGTGTGCTAAGCTCTCTAGAACTTGGATTGGGGCATATTACAAACAAAAGCATTGGCAATAGTATTTGCGTAGGGGCAGGACAGACAAAAGTTGCCTTAGTTTCCTTAGTAAAATGTGTCTTTCTTTTTGCAGACTAGAGTCGCAAAATGGCCACAAAAGCTATAGAGCATAAGACTAAAACCAAATATATGATAGAATAGTACTAGAAACTAACCTGTAAAGTTTATGTTAACACAAAGCATGTGAAATGTGAAGTATGCTCATAATAAGATGTTTACTTCTTTTTTTTTTCCTTTCATTTACTTTTCAATTCTTTCTTTCCTTTTCTTTTGTTTTTGTTTTTGTTTCTGTTTCTTTTTTGTGTTTTCTCTTTTCCTTTCTAGTTTTTAATAACATTTGTGCTTGTAGGAGTTTAGGATGATGGCGAGGTGGTCCCATTGATTGCAATGCTTCATACAGCCTAATTGCTTTTTTTTTTTGGGGGCGATATGTTCTCATGTTTACCTTCAAAAGTTTTCCAATATGTTTTCTCTTGAGGGGGAAAATGCGTTCAATATAAATAACATTCTCTTGTCCATTGAATATGTGTTTTGTTTGATGCTGTTCTCATTCCAAACTCACTTGAAAAATTTATGTACTCAATTACGATGAAACTTCCCTCTTGGAAGAGTGCAAAATACAGACAAACTTATTTTTTGATGTTGAATTTTTTTATGCCTCGTGTCTCTTCAGTTCTTGATTCACCAATAAATTGTTCTTTTGCAGTAATCTCAATATGAAATTTCGGAACTATGTTCCTCATGATAAAGAGCTTCAGGAGGGCAAGCTTGCCCCACCTGTATTGCCTAAATTTGAGGACCCTGTTACTTCTGAACCTCCACAACTAAAAGAGGTAATTTTGTCAGTTCTGCCTTTATATGTTCTTAAACTGGCCTACAATCATACTTTTTGGATCCTTTTCTATTAATTATACTCGATCGTTAAAATTATAGGACCCATTTGTAAATATCGCTCCAAAGAAACCAAACTGGGACCTTAGAAGGGACGTGCAGAAGAAGCTTGATAAGCTTGAAAGACGCACACAGAAAGCACTATTCAAGCTTATGGGTAAGTAGAAGTATTCAATTAAAGGTTAACTTGATAGGTCATTACACTATGCTCGTTCTTTGACTTGCCTTTTCTTGTAGTGTAGCTAGACTATGGATTGAACTTTATTTTCTTTGTTTGTCTGAGAAGATATCTATCTCGGGATGATTGGCATATACATAATACCATTTTGTTTGCTGGTTCTGTTTCTCTTTGATGTATTTAACTTGTGAGCAAATTCCTATGCACCACTGCAGTTATGAACAATTAAGAGATCTTGATTATCATCTTCAAGCATTTGTGTTGAGGTTAAACATGTGTGATGTACTGTTGGTTGCAACATTTTTCTCTAACATTTTCCAACCGAAGTCATTGCACTTGTCTCTGCTGGGATTATAAGCTGTTTTCAATCTTGAAACTTTGAACTTGGCTGGGCTGTTAAAGTTTGGGGGATAGGGATCTTTTGGTATCAAGAGTAGTTGTGGATCTTAGAGTCGGTATAAGCGAGTTTCTTATTTATTCATTTACTTGGGACATTACACTTCAAATAGGAGATGAGGTTTTGAACCTATATTTTTTAGAAGGCATTACATGTCCTATAGTTGTATTCAAATTGATGTAGGTTACCCTAGTTAGCTAGTTATGCATTGAAAGTTGCAAGAGTAGTTATCATGTGTATCATAGAGATGCTAGAAAATTATTTGAAGTCTGAAATTACGGATCCGGTTAAAACATTCAGAGCACCTTGTTTGGTTGCCTTTTTGAACTTTTTGCTTGTTCAATTCTGTGTAGAGGAACAAGAAAAGCAAAAGCAGGCAGCTGAAGGAGACAATGACGAAAATGGTGCAGAGTGATAGTTTCCCACACAATTTATATGCTCTCTGATGTTACAGTCACGGTAATATTCACGACTATATCGACATGCTTGGAGAAAATTGTCCTTAAAGGACAATGGCGTTTCTGGCTTGTAGGGTCAATTTTTCTGTGAGCTTTCTGATCATTCTTAGTTGCAAAACTTTACATTTATAGTTTATCTATGGTGTGGCTCATTGTGTGTTCCTTGACCATTACATCCAATAGAATATCTCATACTGCATAGTGATTTGTAATTTACCTAAACTAAAATTAACTTTGTATTTCACTTTATCTTCTACGTTGTTTTTCTTCTTCATTTTATAATCTATTAGACACAC

mRNA sequence

TCTTGATGGGCCTCTTACGATCCGGGCCTTATATATATATCTCATTCCAAAGAGAAATGCGTCAAAAAGGCTTCTGCGCAAGAAAGAAACACTCGTTTTTCTCCCTAACGCCATTGCTCGAAGCTTCCTGTCTACTTTGTCTGTATTTTACGCACAAAGGCAATGGCTACTGAAGAAGACTCCGTGGAACAAGTTGCTGCAGCACGTAAAGAGAGATTGAAAGCTCTAAGAGCTGCTCAGGAATTGTTAAACAATTCAGATGAGAAGAATTCTGGAGGAGAGGATAAAGAGAATGGAGCTACTGAAGATTCTGATGAAACTAATCTCAATATGAAATTTCGGAACTATGTTCCTCATGATAAAGAGCTTCAGGAGGGCAAGCTTGCCCCACCTGTATTGCCTAAATTTGAGGACCCTGTTACTTCTGAACCTCCACAACTAAAAGAGGACCCATTTGTAAATATCGCTCCAAAGAAACCAAACTGGGACCTTAGAAGGGACGTGCAGAAGAAGCTTGATAAGCTTGAAAGACGCACACAGAAAGCACTATTCAAGCTTATGGAGGAACAAGAAAAGCAAAAGCAGGCAGCTGAAGGAGACAATGACGAAAATGGTGCAGAGTGATAGTTTCCCACACAATTTATATGCTCTCTGATGTTACAGTCACGGTAATATTCACGACTATATCGACATGCTTGGAGAAAATTGTCCTTAAAGGACAATGGCGTTTCTGGCTTGTAGGGTCAATTTTTCTGTGAGCTTTCTGATCATTCTTAGTTGCAAAACTTTACATTTATAGTTTATCTATGGTGTGGCTCATTGTGTGTTCCTTGACCATTACATCCAATAGAATATCTCATACTGCATAGTGATTTGTAATTTACCTAAACTAAAATTAACTTTGTATTTCACTTTATCTTCTACGTTGTTTTTCTTCTTCATTTTATAATCTATTAGACACAC

Coding sequence (CDS)

ATGGCTACTGAAGAAGACTCCGTGGAACAAGTTGCTGCAGCACGTAAAGAGAGATTGAAAGCTCTAAGAGCTGCTCAGGAATTGTTAAACAATTCAGATGAGAAGAATTCTGGAGGAGAGGATAAAGAGAATGGAGCTACTGAAGATTCTGATGAAACTAATCTCAATATGAAATTTCGGAACTATGTTCCTCATGATAAAGAGCTTCAGGAGGGCAAGCTTGCCCCACCTGTATTGCCTAAATTTGAGGACCCTGTTACTTCTGAACCTCCACAACTAAAAGAGGACCCATTTGTAAATATCGCTCCAAAGAAACCAAACTGGGACCTTAGAAGGGACGTGCAGAAGAAGCTTGATAAGCTTGAAAGACGCACACAGAAAGCACTATTCAAGCTTATGGAGGAACAAGAAAAGCAAAAGCAGGCAGCTGAAGGAGACAATGACGAAAATGGTGCAGAGTGA

Protein sequence

MATEEDSVEQVAAARKERLKALRAAQELLNNSDEKNSGGEDKENGATEDSDETNLNMKFRNYVPHDKELQEGKLAPPVLPKFEDPVTSEPPQLKEDPFVNIAPKKPNWDLRRDVQKKLDKLERRTQKALFKLMEEQEKQKQAAEGDNDENGAE*
Homology
BLAST of CSPI01G07650 vs. ExPASy Swiss-Prot
Match: Q8WUD4 (Coiled-coil domain-containing protein 12 OS=Homo sapiens OX=9606 GN=CCDC12 PE=1 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.3e-12
Identity = 52/140 (37.14%), Postives = 89/140 (63.57%), Query Frame = 0

Query: 8   VEQVAAARKERLKALRAAQELLNNSDEKNSGGEDKENGATEDSDETNLNMKFRNYVPHDK 67
           +E+ A  RKERLKALR   E     D+++   + K     E+  E +  ++ RNYVP D+
Sbjct: 11  LEEEALRRKERLKALR---EKTGRKDKEDGEPKTKHLREEEEEGEKHRELRLRNYVPEDE 70

Query: 68  ELQEGKL--APPVL--PKFEDPVTSEPPQ--LKEDPFVNIAPKKPNWDLRRDVQKKLDKL 127
           +L++ ++  A PV    K ++ + +  P+  ++E    N+AP+KP+WDL+RDV KKL+KL
Sbjct: 71  DLKKRRVPQAKPVAVEEKVKEQLEAAKPEPVIEEVDLANLAPRKPDWDLKRDVAKKLEKL 130

Query: 128 ERRTQKALFKLMEEQEKQKQ 142
           ++RTQ+A+ +L+ E+ K ++
Sbjct: 131 KKRTQRAIAELIRERLKGQE 147

BLAST of CSPI01G07650 vs. ExPASy Swiss-Prot
Match: Q8R344 (Coiled-coil domain-containing protein 12 OS=Mus musculus OX=10090 GN=Ccdc12 PE=1 SV=2)

HSP 1 Score: 71.6 bits (174), Expect = 8.6e-12
Identity = 54/142 (38.03%), Postives = 89/142 (62.68%), Query Frame = 0

Query: 8   VEQVAAARKERLKALRAAQELLNNSDEKNSGGEDKENGATEDSDET--NLNMKFRNYVPH 67
           +E+ A  RKERLKALR   E     D ++  GE +     E+ +E   +  ++ RNYVP 
Sbjct: 11  LEEEALRRKERLKALR---EKTGRKDRED--GEPQTKQLREEGEEVGKHRGLRLRNYVPE 70

Query: 68  DKELQEGKL--APPVL--PKFEDPVTSEPPQ--LKEDPFVNIAPKKPNWDLRRDVQKKLD 127
           D++L+  ++  A PV    K ++ + +  P+  ++E    N+AP+KP+WDL+RDV KKL+
Sbjct: 71  DEDLKRRRVPQAKPVAVEEKVKEQLEAAKPEPVIEEVDLANLAPRKPDWDLKRDVAKKLE 130

Query: 128 KLERRTQKALFKLMEEQEKQKQ 142
           KLE+RTQ+A+ +L+ E+ K ++
Sbjct: 131 KLEKRTQRAIAELIRERLKGQE 147

BLAST of CSPI01G07650 vs. ExPASy Swiss-Prot
Match: Q9UU80 (Pre-mRNA-splicing factor cwf18 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=cwf18 PE=1 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 3.9e-04
Identity = 39/137 (28.47%), Postives = 67/137 (48.91%), Query Frame = 0

Query: 7   SVEQVAAARKERLKALRAAQELLNNSDEKNSGGEDKENGATEDSDETNLN-MKFRNYVPH 66
           S+++VA +RK+RL  LR  ++L N                T DS E   N ++ RNY P 
Sbjct: 3   SLDEVAESRKQRLAELRKIKQLENK---------------TRDSQEVQKNVIEHRNYDPE 62

Query: 67  DKELQEGKLAPPVLPKFEDPVTSEPPQ-------------LKEDPFVNIAPKKPNWDLRR 126
            +  + G + PP + +  + ++ E  +             ++E   V + PKKP WDL R
Sbjct: 63  VQAPKMGFVEPPNMIESVEALSKEIEEKTKRKIEEQSSVPVEELDLVTLRPKKPTWDLER 122

Query: 127 DVQKKLDKLERRTQKAL 130
           D+++++  LE + Q A+
Sbjct: 123 DLKERMRSLETQNQNAI 124

BLAST of CSPI01G07650 vs. ExPASy TrEMBL
Match: A0A0A0LR08 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043260 PE=4 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 1.6e-74
Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 0

Query: 1   MATEEDSVEQVAAARKERLKALRAAQELLNNSDEKNSGGEDKENGATEDSDETNLNMKFR 60
           MATEEDSVEQVAAARKERLKALRAAQELLNNSDEKNSGGEDKENGATEDSDETNLNMKFR
Sbjct: 42  MATEEDSVEQVAAARKERLKALRAAQELLNNSDEKNSGGEDKENGATEDSDETNLNMKFR 101

Query: 61  NYVPHDKELQEGKLAPPVLPKFEDPVTSEPPQLKEDPFVNIAPKKPNWDLRRDVQKKLDK 120
           NYVPHDKELQEGKLAPPVLPKFEDPVTSEPPQLKEDPFVNIAPKKPNWDLRRDVQKKLDK
Sbjct: 102 NYVPHDKELQEGKLAPPVLPKFEDPVTSEPPQLKEDPFVNIAPKKPNWDLRRDVQKKLDK 161

Query: 121 LERRTQKALFKLMEEQEKQKQAAEGDNDENGAE 154
           LERRTQKALFKLMEEQEKQKQAAEGDNDENGAE
Sbjct: 162 LERRTQKALFKLMEEQEKQKQAAEGDNDENGAE 194

BLAST of CSPI01G07650 vs. ExPASy TrEMBL
Match: A0A1S3ATB1 (coiled-coil domain-containing protein 12 OS=Cucumis melo OX=3656 GN=LOC103482658 PE=4 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.4e-68
Identity = 143/153 (93.46%), Postives = 148/153 (96.73%), Query Frame = 0

Query: 1   MATEEDSVEQVAAARKERLKALRAAQELLNNSDEKNSGGEDKENGATEDSDETNLNMKFR 60
           MATEEDS EQVAAARKERLKALRAAQELLN SDEKNS GEDKENGAT D+DETNLNMKFR
Sbjct: 1   MATEEDSFEQVAAARKERLKALRAAQELLNASDEKNSVGEDKENGATADTDETNLNMKFR 60

Query: 61  NYVPHDKELQEGKLAPPVLPKFEDPVTSEPPQLKEDPFVNIAPKKPNWDLRRDVQKKLDK 120
           NYVPHDKELQEGKLAPPVLPKFEDPVT+EPPQ KEDPFVNIAPKKPNWDLRRDVQKKLDK
Sbjct: 61  NYVPHDKELQEGKLAPPVLPKFEDPVTTEPPQQKEDPFVNIAPKKPNWDLRRDVQKKLDK 120

Query: 121 LERRTQKALFKLMEEQEKQKQAAEGDNDENGAE 154
           LERRTQKAL+KLMEEQEKQKQAAEGD+D+NGAE
Sbjct: 121 LERRTQKALYKLMEEQEKQKQAAEGDDDQNGAE 153

BLAST of CSPI01G07650 vs. ExPASy TrEMBL
Match: A0A5D3BJ95 (Coiled-coil domain-containing protein 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002830 PE=4 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.4e-68
Identity = 143/153 (93.46%), Postives = 148/153 (96.73%), Query Frame = 0

Query: 1   MATEEDSVEQVAAARKERLKALRAAQELLNNSDEKNSGGEDKENGATEDSDETNLNMKFR 60
           MATEEDS EQVAAARKERLKALRAAQELLN SDEKNS GEDKENGAT D+DETNLNMKFR
Sbjct: 1   MATEEDSFEQVAAARKERLKALRAAQELLNASDEKNSVGEDKENGATADTDETNLNMKFR 60

Query: 61  NYVPHDKELQEGKLAPPVLPKFEDPVTSEPPQLKEDPFVNIAPKKPNWDLRRDVQKKLDK 120
           NYVPHDKELQEGKLAPPVLPKFEDPVT+EPPQ KEDPFVNIAPKKPNWDLRRDVQKKLDK
Sbjct: 61  NYVPHDKELQEGKLAPPVLPKFEDPVTTEPPQQKEDPFVNIAPKKPNWDLRRDVQKKLDK 120

Query: 121 LERRTQKALFKLMEEQEKQKQAAEGDNDENGAE 154
           LERRTQKAL+KLMEEQEKQKQAAEGD+D+NGAE
Sbjct: 121 LERRTQKALYKLMEEQEKQKQAAEGDDDQNGAE 153

BLAST of CSPI01G07650 vs. ExPASy TrEMBL
Match: A0A6J1DLK0 (coiled-coil domain-containing protein 12 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022269 PE=4 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 2.0e-64
Identity = 133/153 (86.93%), Postives = 145/153 (94.77%), Query Frame = 0

Query: 1   MATEEDSVEQVAAARKERLKALRAAQELLNNSDEKNSGGEDKENGATEDSDETNLNMKFR 60
           MATEEDSVEQVAAARKERL+ALRAAQELLN  DEK SGGEDK+NG  ED++ETNL+MKFR
Sbjct: 1   MATEEDSVEQVAAARKERLRALRAAQELLNTPDEKTSGGEDKDNGVIEDTEETNLSMKFR 60

Query: 61  NYVPHDKELQEGKLAPPVLPKFEDPVTSEPPQLKEDPFVNIAPKKPNWDLRRDVQKKLDK 120
           NYVPHDKELQEGKLAPPVLPKFEDPV +EPPQLKEDPFVNIAPKKPNWDLRRDVQKKLDK
Sbjct: 61  NYVPHDKELQEGKLAPPVLPKFEDPVANEPPQLKEDPFVNIAPKKPNWDLRRDVQKKLDK 120

Query: 121 LERRTQKALFKLMEEQEKQKQAAEGDNDENGAE 154
           LERRTQKAL+KLM+EQEKQK++AEGD+ +NG E
Sbjct: 121 LERRTQKALYKLMKEQEKQKESAEGDDVQNGTE 153

BLAST of CSPI01G07650 vs. ExPASy TrEMBL
Match: A0A6J1JYP1 (coiled-coil domain-containing protein 12-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490058 PE=4 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 2.6e-64
Identity = 133/153 (86.93%), Postives = 143/153 (93.46%), Query Frame = 0

Query: 1   MATEEDSVEQVAAARKERLKALRAAQELLNNSDEKNSGGEDKENGATEDSDETNLNMKFR 60
           MATEEDSVEQVAAAR+ERL+ALR AQELLN  DEK SGGED+ENG   D++ETNL MKFR
Sbjct: 1   MATEEDSVEQVAAARQERLRALRTAQELLNAPDEKTSGGEDQENGTAADTEETNLGMKFR 60

Query: 61  NYVPHDKELQEGKLAPPVLPKFEDPVTSEPPQLKEDPFVNIAPKKPNWDLRRDVQKKLDK 120
           NYVPHDKELQEGKLAPPVLPKFEDPV +EPPQLKEDPFVNIAPKKPNWDLRRDVQKKLDK
Sbjct: 61  NYVPHDKELQEGKLAPPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQKKLDK 120

Query: 121 LERRTQKALFKLMEEQEKQKQAAEGDNDENGAE 154
           LERRTQKAL+KLMEEQEKQKQ+AEGD+D+NG E
Sbjct: 121 LERRTQKALYKLMEEQEKQKQSAEGDDDQNGTE 153

BLAST of CSPI01G07650 vs. NCBI nr
Match: XP_004137537.1 (coiled-coil domain-containing protein 12 [Cucumis sativus] >XP_031743618.1 coiled-coil domain-containing protein 12 [Cucumis sativus] >KGN64229.2 hypothetical protein Csa_013562 [Cucumis sativus])

HSP 1 Score: 288.5 bits (737), Expect = 3.4e-74
Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 0

Query: 1   MATEEDSVEQVAAARKERLKALRAAQELLNNSDEKNSGGEDKENGATEDSDETNLNMKFR 60
           MATEEDSVEQVAAARKERLKALRAAQELLNNSDEKNSGGEDKENGATEDSDETNLNMKFR
Sbjct: 1   MATEEDSVEQVAAARKERLKALRAAQELLNNSDEKNSGGEDKENGATEDSDETNLNMKFR 60

Query: 61  NYVPHDKELQEGKLAPPVLPKFEDPVTSEPPQLKEDPFVNIAPKKPNWDLRRDVQKKLDK 120
           NYVPHDKELQEGKLAPPVLPKFEDPVTSEPPQLKEDPFVNIAPKKPNWDLRRDVQKKLDK
Sbjct: 61  NYVPHDKELQEGKLAPPVLPKFEDPVTSEPPQLKEDPFVNIAPKKPNWDLRRDVQKKLDK 120

Query: 121 LERRTQKALFKLMEEQEKQKQAAEGDNDENGAE 154
           LERRTQKALFKLMEEQEKQKQAAEGDNDENGAE
Sbjct: 121 LERRTQKALFKLMEEQEKQKQAAEGDNDENGAE 153

BLAST of CSPI01G07650 vs. NCBI nr
Match: XP_038894163.1 (coiled-coil domain-containing protein 12 [Benincasa hispida])

HSP 1 Score: 269.6 bits (688), Expect = 1.6e-68
Identity = 142/153 (92.81%), Postives = 150/153 (98.04%), Query Frame = 0

Query: 1   MATEEDSVEQVAAARKERLKALRAAQELLNNSDEKNSGGEDKENGATEDSDETNLNMKFR 60
           MATEEDSVEQVAAARKERL+ALRAAQELLN SDEKNSGGEDKENGAT D++ETNLNMKFR
Sbjct: 1   MATEEDSVEQVAAARKERLRALRAAQELLNASDEKNSGGEDKENGATADAEETNLNMKFR 60

Query: 61  NYVPHDKELQEGKLAPPVLPKFEDPVTSEPPQLKEDPFVNIAPKKPNWDLRRDVQKKLDK 120
           NYVPHDKELQEGKLAPPVLPKFEDPVT+EP QLKEDPFVNIAPKKPNWDLRRDVQKKLDK
Sbjct: 61  NYVPHDKELQEGKLAPPVLPKFEDPVTTEPAQLKEDPFVNIAPKKPNWDLRRDVQKKLDK 120

Query: 121 LERRTQKALFKLMEEQEKQKQAAEGDNDENGAE 154
           LERRTQKAL+KLMEEQEKQKQ+AEGD+D+NGAE
Sbjct: 121 LERRTQKALYKLMEEQEKQKQSAEGDDDQNGAE 153

BLAST of CSPI01G07650 vs. NCBI nr
Match: XP_008437152.1 (PREDICTED: coiled-coil domain-containing protein 12 [Cucumis melo] >XP_008437160.1 PREDICTED: coiled-coil domain-containing protein 12 [Cucumis melo] >TYJ99079.1 coiled-coil domain-containing protein 12 [Cucumis melo var. makuwa])

HSP 1 Score: 268.9 bits (686), Expect = 2.8e-68
Identity = 143/153 (93.46%), Postives = 148/153 (96.73%), Query Frame = 0

Query: 1   MATEEDSVEQVAAARKERLKALRAAQELLNNSDEKNSGGEDKENGATEDSDETNLNMKFR 60
           MATEEDS EQVAAARKERLKALRAAQELLN SDEKNS GEDKENGAT D+DETNLNMKFR
Sbjct: 1   MATEEDSFEQVAAARKERLKALRAAQELLNASDEKNSVGEDKENGATADTDETNLNMKFR 60

Query: 61  NYVPHDKELQEGKLAPPVLPKFEDPVTSEPPQLKEDPFVNIAPKKPNWDLRRDVQKKLDK 120
           NYVPHDKELQEGKLAPPVLPKFEDPVT+EPPQ KEDPFVNIAPKKPNWDLRRDVQKKLDK
Sbjct: 61  NYVPHDKELQEGKLAPPVLPKFEDPVTTEPPQQKEDPFVNIAPKKPNWDLRRDVQKKLDK 120

Query: 121 LERRTQKALFKLMEEQEKQKQAAEGDNDENGAE 154
           LERRTQKAL+KLMEEQEKQKQAAEGD+D+NGAE
Sbjct: 121 LERRTQKALYKLMEEQEKQKQAAEGDDDQNGAE 153

BLAST of CSPI01G07650 vs. NCBI nr
Match: KAG6584286.1 (Coiled-coil domain-containing protein 12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 263.1 bits (671), Expect = 1.5e-66
Identity = 138/153 (90.20%), Postives = 147/153 (96.08%), Query Frame = 0

Query: 1   MATEEDSVEQVAAARKERLKALRAAQELLNNSDEKNSGGEDKENGATEDSDETNLNMKFR 60
           MATE+DSVEQVAAARKERL+ALRAAQELLN SDEKNSGGEDKENGATED +ETNL+MKFR
Sbjct: 1   MATEDDSVEQVAAARKERLRALRAAQELLNTSDEKNSGGEDKENGATEDPEETNLSMKFR 60

Query: 61  NYVPHDKELQEGKLAPPVLPKFEDPVTSEPPQLKEDPFVNIAPKKPNWDLRRDVQKKLDK 120
           NYVPHDKELQEGKL PPVLPKFEDPV +EPPQLKEDPFVNIAPKKPNWDLRRDVQKKLDK
Sbjct: 61  NYVPHDKELQEGKLVPPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQKKLDK 120

Query: 121 LERRTQKALFKLMEEQEKQKQAAEGDNDENGAE 154
           LERRTQKAL+KLMEEQEKQKQ+AEGD+D+N  E
Sbjct: 121 LERRTQKALYKLMEEQEKQKQSAEGDDDQNITE 153

BLAST of CSPI01G07650 vs. NCBI nr
Match: XP_023520254.1 (coiled-coil domain-containing protein 12-like [Cucurbita pepo subsp. pepo] >XP_023520255.1 coiled-coil domain-containing protein 12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 257.7 bits (657), Expect = 6.4e-65
Identity = 138/156 (88.46%), Postives = 147/156 (94.23%), Query Frame = 0

Query: 1   MATEEDSVEQVAAARKERLKALRAAQELLNNSDEKNSGGEDKENGAT---EDSDETNLNM 60
           MATE+DSVEQVAAARKERL+ALRAAQELLN SDEKNSGGEDKENGAT   ED +ETNL+M
Sbjct: 1   MATEDDSVEQVAAARKERLRALRAAQELLNTSDEKNSGGEDKENGATEDPEDPEETNLSM 60

Query: 61  KFRNYVPHDKELQEGKLAPPVLPKFEDPVTSEPPQLKEDPFVNIAPKKPNWDLRRDVQKK 120
           KFRNYVPHDKELQEGKL PPVLPKFEDPV +EPPQLKEDPFVNIAPKKPNWDLRRDVQKK
Sbjct: 61  KFRNYVPHDKELQEGKLVPPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQKK 120

Query: 121 LDKLERRTQKALFKLMEEQEKQKQAAEGDNDENGAE 154
           LDKLERRTQKAL+KLMEEQEKQKQ+AEGD+D+N  E
Sbjct: 121 LDKLERRTQKALYKLMEEQEKQKQSAEGDDDQNITE 156

BLAST of CSPI01G07650 vs. TAIR 10
Match: AT3G05070.1 (CONTAINS InterPro DOMAIN/s: mRNA splicing factor, Cwf18 (InterPro:IPR013169); Has 292 Blast hits to 292 proteins in 153 species: Archae - 0; Bacteria - 0; Metazoa - 118; Fungi - 83; Plants - 38; Viruses - 11; Other Eukaryotes - 42 (source: NCBI BLink). )

HSP 1 Score: 176.0 bits (445), Expect = 2.3e-44
Identity = 101/150 (67.33%), Postives = 116/150 (77.33%), Query Frame = 0

Query: 1   MATEEDSVEQVAAARKERLKALRAAQELLNNSDEKNSGGEDKENGATEDSDETNLNMKFR 60
           MA+E++S+EQ AAARKE LKALRAAQEL           E KE+G  E  +E    MKFR
Sbjct: 1   MASEDESIEQKAAARKEALKALRAAQEL----------SETKEDGEDEAVEEDGPAMKFR 60

Query: 61  NYVPHDKELQEGKLAPPVLPKFEDPVTSEPP--QLKEDPFVNIAPKKPNWDLRRDVQKKL 120
           NYVP  KELQ+GKLAPP LPKFEDP+ + PP  + KEDPFVNIAPKKPNWDLRRDVQKKL
Sbjct: 61  NYVPQAKELQDGKLAPPELPKFEDPIVALPPAVEKKEDPFVNIAPKKPNWDLRRDVQKKL 120

Query: 121 DKLERRTQKALFKLMEEQEKQKQAAEGDND 149
           DKLERRTQKA+ KLMEEQEK+++ AE D +
Sbjct: 121 DKLERRTQKAMHKLMEEQEKEREMAEADGN 140

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8WUD41.3e-1237.14Coiled-coil domain-containing protein 12 OS=Homo sapiens OX=9606 GN=CCDC12 PE=1 ... [more]
Q8R3448.6e-1238.03Coiled-coil domain-containing protein 12 OS=Mus musculus OX=10090 GN=Ccdc12 PE=1... [more]
Q9UU803.9e-0428.47Pre-mRNA-splicing factor cwf18 OS=Schizosaccharomyces pombe (strain 972 / ATCC 2... [more]
Match NameE-valueIdentityDescription
A0A0A0LR081.6e-74100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043260 PE=4 SV=1[more]
A0A1S3ATB11.4e-6893.46coiled-coil domain-containing protein 12 OS=Cucumis melo OX=3656 GN=LOC103482658... [more]
A0A5D3BJ951.4e-6893.46Coiled-coil domain-containing protein 12 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A6J1DLK02.0e-6486.93coiled-coil domain-containing protein 12 isoform X1 OS=Momordica charantia OX=36... [more]
A0A6J1JYP12.6e-6486.93coiled-coil domain-containing protein 12-like isoform X1 OS=Cucurbita maxima OX=... [more]
Match NameE-valueIdentityDescription
XP_004137537.13.4e-74100.00coiled-coil domain-containing protein 12 [Cucumis sativus] >XP_031743618.1 coile... [more]
XP_038894163.11.6e-6892.81coiled-coil domain-containing protein 12 [Benincasa hispida][more]
XP_008437152.12.8e-6893.46PREDICTED: coiled-coil domain-containing protein 12 [Cucumis melo] >XP_008437160... [more]
KAG6584286.11.5e-6690.20Coiled-coil domain-containing protein 12, partial [Cucurbita argyrosperma subsp.... [more]
XP_023520254.16.4e-6588.46coiled-coil domain-containing protein 12-like [Cucurbita pepo subsp. pepo] >XP_0... [more]
Match NameE-valueIdentityDescription
AT3G05070.12.3e-4467.33CONTAINS InterPro DOMAIN/s: mRNA splicing factor, Cwf18 (InterPro:IPR013169); Ha... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 118..153
NoneNo IPR availableCOILSCoilCoilcoord: 11..31
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 77..101
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 24..56
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 134..153
IPR013169mRNA splicing factor Cwf18-likePFAMPF08315cwf18coord: 8..135
e-value: 1.2E-33
score: 116.7
IPR013169mRNA splicing factor Cwf18-likePANTHERPTHR31551PRE-MRNA-SPLICING FACTOR CWF18coord: 1..146

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G07650.1CSPI01G07650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0071014 post-mRNA release spliceosomal complex
cellular_component GO:0005684 U2-type spliceosomal complex