CSPI01G05950 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G05950
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	SNAP25 homologous protein SNAP33-like
Location	Chr1: 3742084 .. 3745510 (-)
RNA-Seq Expression	CSPI01G05950
Synteny	CSPI01G05950

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCCTAATGTTTGACCAAATCCATCGACGCCATTTGTTCCTTTTTCCTTTTTAATCCAAAAGGTCAATTTAGCGGTTTATGAGGTCAAGGTTGTCTTCTTCATTGCTCATCTTCTTTCTTCGCAGGTGCCCTTTTCAACAATTTACTGATACGCGGTTTCTTTATTGATTTTTCTTCTCATTGCTCAAGTCCTTTATTGCCACTTTAAATTCTACAATTGGGTTCTGATTTTTGCAGTTTCTTTGATTTTGGGTTCGTGAAATAGTTCCATTCTCTTCGTTTTCTGAACTCTCTCAAGGTACTTTAGTTTTTCTTTTCACTGTTTTCTTTTCTTTCTTGGATTTTTGTATCTGGGTTTTGATCACCATCTTCCTCCTTGGACTTTTACATGATTTTCTTAGTTAACTTGTTGTACCCTTTTAGTTTTCGATCGTGCCATTAACTGTTCTGATCTACATCCATCTATTTCTTCTTATTAGATCAATTTGTATCCAACCTGTTTGATGTTTTACTTAAATAAAAAGCTAAATCCTAGAACATCTCTTCCTCGTTTTAACTTTAATGAAATTGATTGTTTTGATAAATTTGTCTCTTGTTGTATTTTATTTCTTCTGCTGACATGATTGTGGTGCCTTGCTTTTTCAGGAAAAAACCATTAACCTATTGGATTACTTTGTTCCGTTTACATTAAAGCCAATTTTGACATTCTAGCTATCGGGAAAATGTTTGGTTCAAGGAAGGTTCATTTTAGGTTAGGAAAACATAGTTCCACCCCAAACCCTATTGAGTCTGATGATGATGTTAAAGCTAAGCAAAACCTGGTTTCTTCACAAAGAACTTCCTCAGACCCTTCATTACTGACGGAGAAGTTTAGTTCCAATCCTTTTGATGATGATCATGGACATGCCCCCTCTTCTTCAAGTTCAACAACTTGGTCAGAAAGGAACAAGTACAAGAATGATTTTCGTGACTCAGGAGGAGTAGAAAACCAATCAGTACAGGAACTGGAGAATTATGCTGTTTATAAAGCTGAAGAGACAACAAAAGCAGTGAATGGCTGTTTAAAGATTGCTGAAGAGATAAGGGAGGATGCTACTCAAACTTTGGTTGCATTGCATCACCAGGGTGAGCAAATTACCAGGACTCATATGGTTGCTGCTGACATCGATTATGATCTCAGTCGGGTATGTTCGAACTACGATTTGTTCACTTTTGAAATTCTTGGTGTTTCATGGTTTAGAAAATTACTAGCGTTAACTTTACATGTATAATTATAAGCATTTCTTTTTTTATTTGACGAATTACTTTTCTGAACCTTTTTCCTTTCATGAATGCAATATCAGGGAGAGAAACTGCTGGGAAATCTAGGAGGAATATTCTCTAAGACTTGGAAGCCAAAGAAAACTCGACCAATCTCAGGACCTATTATAAGCTCAGGTTAGCTTCTTTTCTTTCCATGTTCACCATGATTGTTCTGCTCATGAGATTTTGGGTTAGATTGGCACTTGGTGAATTCTGTCCAACTGATCTGTTTATTTGCTTATGCATAATTTGTAGATGATTTAGTAAGAAGAAAGGGCAACCATTTAGAGCAGAAGGAAAGGTTAGGATTGACTCATCATAAGGAGAGGACCAGTACAAAAGCTGCATTTGCTGAACCTACCAATGCAATTCAAAAAGTTGAGGTTTGTATTTGCTATCTATGAACAATTACTTAAAAGCAGTCATTGACATTTAAACAGGGCATGAATTATTGTATTTGCTTTTCGCTTAAGTATAGTCTTAAACAATTCTGTACCATGTTGCCCAAAAAGCATATTTATCATTCCCAAGGCGCAGTATCATTCATCTCACATAAGCTGTGTGTTTCAAAATTATACATGAAACAGGTAGAAAAGATGAAGCAAGATGATGCACTATCTGATATGAGCGATATCTTGGTACAACTGAAGGAAATGGCTGTTGACATGGGTAGTGAAATGGACAGGTTTGTTTCCATCTTATATCTTCCTGAAAAACTTGGATCTTTCTTGCAAGAGAAAAACAAAAGGACTAGGTGTATATTGGAAATTGAGATGAGCTGTTGTGCTTATTACAGGCAGAACGTTGCTCTGGATCATGCTTCTGTTGATGTTGAGGTGCTTGGTGACCGAGTTAAAGATGCCAACCGACGCGGTCGTCGTTTGCTTGGAAAATAGAGTTCAAGGGCCTCACTGTTGCAGTTGGCCGGCTGATGCTTATTTGCATCTTCCGTTTTCTTGGCTTTGTTTTCTTTTTGCTTTGAGGCATATCATTATGCCCCCTGTTCTTGATGACATTTTTCTTTACTTGAGATCAATAAATACTTCATTAGTACTAGAAAGTAACTGCACTGATTCAATAGTTCCAAGCATAATTCACCTTGAGCCATGTATACCAACTGTTAATCAACTAGAATCGTGCATTTTCGAACACGGGAATTTTTAATAATCCAAGTTCTGATCGGTCTAAAGTGATACGAACAAAGATGCAACAATGCCTTCTTTATAAAGAACTTAAATTTAAGCAAACAAGTATTCTTCATACTTTCAAGCTGAAATGAGCTCAAACGAGGTGAAATAAGTTCCTGGCAAATCAATTCTATTCCAGGATACGTATGAAAACATTATGGTATCAGCTAAATCATAAGAGTCCTGCAAAAGATCAGTTGCAAGATTCAACCTCAAAGCAACCAATTTTTTTTATTGAGTTAAATATTGAAATGGACAAAATATTTCTCCAAAGTAATAACGTTCATATCCCAAACATGCTTCATTAACATTCTGAATTTCAATTGGATGATCAGACATTATTTCATTAAAACCCAGAAGAGAAACCCAGGAATGCATACCTACGACTGGATAAATTGGCGAAAAGGGAAAAAAAAATCCCTAATTTCCCTTTCGGAATTAAACTCGCAAGAGAATTTCAGGATGGGATGGAAAATACTTTGCTCATAATTAATAACAACAGAACGTAAGTTTACAGTTCAATCCTCACAAAAATTGTCAAACACTACAAATTCATCGAACCATAAAAAAAGAAAAAGAAAAAAAAGAAATGGAAATCAATGGTTGACATCCATGATACTCTTGACCTTAGGCGCGTAAAGAGAGTAAACAATGGCCTGGCGCTTGGCAACCTCGAGCTGAGCTCTGCCGCCAGCGTAGACGGAGGAGATGGATGAAGCGTCGGAGAGGGTCTGATTTTCTCGGAAAGAATCAATGGTACGGCGTTTGATGTATTCTCTGATGTTGTAATCAGGAAATTGACGGGCGGTTCGCAGGAGAGAACGAAAGAGCGAGAGGATCTCAGAGGTGGACGGAGTTGCCATGTGTGGAGGAAGAGCTTAGGGATAGAGATTGTAATGTACATAATACTATATATATATGGATGTATACTATTATGT

mRNA sequence

TCCCTAATGTTTGACCAAATCCATCGACGCCATTTGTTCCTTTTTCCTTTTTAATCCAAAAGGTCAATTTAGCGGTTTATGAGGTCAAGGTTGTCTTCTTCATTGCTCATCTTCTTTCTTCGCAGTTTCTTTGATTTTGGGTTCGTGAAATAGTTCCATTCTCTTCGTTTTCTGAACTCTCTCAAGGAAAAAACCATTAACCTATTGGATTACTTTGTTCCGTTTACATTAAAGCCAATTTTGACATTCTAGCTATCGGGAAAATGTTTGGTTCAAGGAAGGTTCATTTTAGGTTAGGAAAACATAGTTCCACCCCAAACCCTATTGAGTCTGATGATGATGTTAAAGCTAAGCAAAACCTGGTTTCTTCACAAAGAACTTCCTCAGACCCTTCATTACTGACGGAGAAGTTTAGTTCCAATCCTTTTGATGATGATCATGGACATGCCCCCTCTTCTTCAAGTTCAACAACTTGGTCAGAAAGGAACAAGTACAAGAATGATTTTCGTGACTCAGGAGGAGTAGAAAACCAATCAGTACAGGAACTGGAGAATTATGCTGTTTATAAAGCTGAAGAGACAACAAAAGCAGTGAATGGCTGTTTAAAGATTGCTGAAGAGATAAGGGAGGATGCTACTCAAACTTTGGTTGCATTGCATCACCAGGGTGAGCAAATTACCAGGACTCATATGGTTGCTGCTGACATCGATTATGATCTCAGTCGGGGAGAGAAACTGCTGGGAAATCTAGGAGGAATATTCTCTAAGACTTGGAAGCCAAAGAAAACTCGACCAATCTCAGGACCTATTATAAGCTCAGATGATTTAGTAAGAAGAAAGGGCAACCATTTAGAGCAGAAGGAAAGGTTAGGATTGACTCATCATAAGGAGAGGACCAGTACAAAAGCTGCATTTGCTGAACCTACCAATGCAATTCAAAAAGTTGAGGTAGAAAAGATGAAGCAAGATGATGCACTATCTGATATGAGCGATATCTTGGTACAACTGAAGGAAATGGCTGTTGACATGGGTAGTGAAATGGACAGGTTTGTTTCCATCTTATATCTTCCTGAAAAACTTGGATCTTTCTTGCAAGAGAAAAACAAAAGGACTAGGTGTATATTGGAAATTGAGATGAGCTGTTGTGCTTATTACAGGCAGAACGTTGCTCTGGATCATGCTTCTGTTGATGTTGAGGTGCTTGGTGACCGAGTTAAAGATGCCAACCGACGCGGTCGTCGTTTGCTTGGAAAATAGAGTTCAAGGGCCTCACTGTTGCAGTTGGCCGGCTGATGCTTATTTGCATCTTCCGTTTTCTTGGCTTTGTTTTCTTTTTGCTTTGAGGCATATCATTATGCCCCCTGTTCTTGATGACATTTTTCTTTACTTGAGATCAATAAATACTTCATTAGTACTAGAAAGTAACTGCACTGATTCAATAGTTCCAAGCATAATTCACCTTGAGCCATGTATACCAACTGTTAATCAACTAGAATCGTGCATTTTCGAACACGGGAATTTTTAATAATCCAAGTTCTGATCGGTCTAAAGTGATACGAACAAAGATGCAACAATGCCTTCTTTATAAAGAACTTAAATTTAAGCAAACAAGTATTCTTCATACTTTCAAGCTGAAATGAGCTCAAACGAGGTGAAATAAGTTCCTGGCAAATCAATTCTATTCCAGGATACGTATGAAAACATTATGGTATCAGCTAAATCATAAGAGTCCTGCAAAAGATCAGTTGCAAGATTCAACCTCAAAGCAACCAATTTTTTTTATTGAGTTAAATATTGAAATGGACAAAATATTTCTCCAAAGTAATAACGTTCATATCCCAAACATGCTTCATTAACATTCTGAATTTCAATTGGATGATCAGACATTATTTCATTAAAACCCAGAAGAGAAACCCAGGAATGCATACCTACGACTGGATAAATTGGCGAAAAGGGAAAAAAAAATCCCTAATTTCCCTTTCGGAATTAAACTCGCAAGAGAATTTCAGGATGGGATGGAAAATACTTTGCTCATAATTAATAACAACAGAACGTAAGTTTACAGTTCAATCCTCACAAAAATTGTCAAACACTACAAATTCATCGAACCATAAAAAAAGAAAAAGAAAAAAAAGAAATGGAAATCAATGGTTGACATCCATGATACTCTTGACCTTAGGCGCGTAAAGAGAGTAAACAATGGCCTGGCGCTTGGCAACCTCGAGCTGAGCTCTGCCGCCAGCGTAGACGGAGGAGATGGATGAAGCGTCGGAGAGGGTCTGATTTTCTCGGAAAGAATCAATGGTACGGCGTTTGATGTATTCTCTGATGTTGTAATCAGGAAATTGACGGGCGGTTCGCAGGAGAGAACGAAAGAGCGAGAGGATCTCAGAGGTGGACGGAGTTGCCATGTGTGGAGGAAGAGCTTAGGGATAGAGATTGTAATGTACATAATACTATATATATATGGATGTATACTATTATGT

Coding sequence (CDS)

ATGTTTGGTTCAAGGAAGGTTCATTTTAGGTTAGGAAAACATAGTTCCACCCCAAACCCTATTGAGTCTGATGATGATGTTAAAGCTAAGCAAAACCTGGTTTCTTCACAAAGAACTTCCTCAGACCCTTCATTACTGACGGAGAAGTTTAGTTCCAATCCTTTTGATGATGATCATGGACATGCCCCCTCTTCTTCAAGTTCAACAACTTGGTCAGAAAGGAACAAGTACAAGAATGATTTTCGTGACTCAGGAGGAGTAGAAAACCAATCAGTACAGGAACTGGAGAATTATGCTGTTTATAAAGCTGAAGAGACAACAAAAGCAGTGAATGGCTGTTTAAAGATTGCTGAAGAGATAAGGGAGGATGCTACTCAAACTTTGGTTGCATTGCATCACCAGGGTGAGCAAATTACCAGGACTCATATGGTTGCTGCTGACATCGATTATGATCTCAGTCGGGGAGAGAAACTGCTGGGAAATCTAGGAGGAATATTCTCTAAGACTTGGAAGCCAAAGAAAACTCGACCAATCTCAGGACCTATTATAAGCTCAGATGATTTAGTAAGAAGAAAGGGCAACCATTTAGAGCAGAAGGAAAGGTTAGGATTGACTCATCATAAGGAGAGGACCAGTACAAAAGCTGCATTTGCTGAACCTACCAATGCAATTCAAAAAGTTGAGGTAGAAAAGATGAAGCAAGATGATGCACTATCTGATATGAGCGATATCTTGGTACAACTGAAGGAAATGGCTGTTGACATGGGTAGTGAAATGGACAGGTTTGTTTCCATCTTATATCTTCCTGAAAAACTTGGATCTTTCTTGCAAGAGAAAAACAAAAGGACTAGGTGTATATTGGAAATTGAGATGAGCTGTTGTGCTTATTACAGGCAGAACGTTGCTCTGGATCATGCTTCTGTTGATGTTGAGGTGCTTGGTGACCGAGTTAAAGATGCCAACCGACGCGGTCGTCGTTTGCTTGGAAAATAG

Protein sequence

MFGSRKVHFRLGKHSSTPNPIESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHGHAPSSSSSTTWSERNKYKNDFRDSGGVENQSVQELENYAVYKAEETTKAVNGCLKIAEEIREDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGIFSKTWKPKKTRPISGPIISSDDLVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSDMSDILVQLKEMAVDMGSEMDRFVSILYLPEKLGSFLQEKNKRTRCILEIEMSCCAYYRQNVALDHASVDVEVLGDRVKDANRRGRRLLGK*

Homology

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BLAST of CSPI01G05950 vs. ExPASy Swiss-Prot
Match: Q9S7P9 (SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 4.5e-86
Identity = 177/303 (58.42%), Postives = 223/303 (73.60%), Query Frame = 0


Query: 1   MFGSRKVHFRLGKH------SSTPNPIESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNP 60
           MFG RK    L KH      SS PNP +SDD+   K  L  S+RT+S+PSL      +NP
Sbjct: 1   MFGLRKSPANLPKHNSVDLKSSKPNPFDSDDESDNKHTLNPSKRTTSEPSLAD---MTNP 60

Query: 61  FDDDH---GHAPSSSSSTTWSERNKYKNDFRDSGGVENQSVQELENYAVYKAEETTKAVN 120
           F  +    G + SS  S   + + +YKN+FRDSGG+ENQSVQELE YAVYKAEETTK+V 
Sbjct: 61  FGGERVQKGDSSSSKQSLFSNSKYQYKNNFRDSGGIENQSVQELEGYAVYKAEETTKSVQ 120

Query: 121 GCLKIAEEIREDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGIFSKTWK 180
           GCLK+AE+IR DAT+TLV LH QGEQITRTH  A +ID+DLSRGEKLLG+LGG+FSKTWK
Sbjct: 121 GCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGGMFSKTWK 180

Query: 181 PKKTRPISGPIISSDDLVRRKGNHLEQKERLGL-THHKERTSTKAAFAEPTNAIQKVEVE 240
           PKKTRPI+GP+++ DD   R+ NHLE++E+LGL +  + ++ T+    E  +A Q+VE+E
Sbjct: 181 PKKTRPINGPVVTRDDSPTRRVNHLEKREKLGLNSAPRGQSRTREPLPESADAYQRVEME 240

Query: 241 KMKQDDALSDMSDILVQLKEMAVDMGSEMDRQNVALDHASVDVEVLGDRVKDANRRGRRL 294
           K KQDD LSD+SDIL +LK MAVDMGSE+++QN  LDH   DV+ L  RV+ +N+RGRRL
Sbjct: 241 KAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQSNQRGRRL 300

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BLAST of CSPI01G05950 vs. ExPASy Swiss-Prot
Match: Q9LMG8 (Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNAP30 PE=3 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 2.4e-71
Identity = 150/275 (54.55%), Postives = 189/275 (68.73%), Query Frame = 0


Query: 19  NPIESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHGHAPSSSSSTTWSERNKYK 78
           N + ++        + + +RTSS+P L+T       FDDD                +KYK
Sbjct: 11  NKLPNESSNNKGGTITAGRRTSSEPILITP-----DFDDD----------------DKYK 70

Query: 79  NDFRDSGGVENQSVQELENYAVYKAEETTKAVNGCLKIAEEIREDATQTLVALHHQGEQI 138
           N F DSGG+++Q+ +ELE YAVYKAEETTK VN CLKIAE+IR D  +TL  LH QGEQI
Sbjct: 71  NGFNDSGGLQSQTTEELEKYAVYKAEETTKGVNNCLKIAEDIRSDGARTLEMLHQQGEQI 130

Query: 139 TRTHMVAADIDYDLSRGEKLLGNLGGIFSKTWKPKKTRPISGPIISSDDLVRRKGNHLEQ 198
            RTH +A D+D DLSRGEKLL NLGG+FSK WKPKKT+ I+GP+I+ D   ++  NH E+
Sbjct: 131 NRTHEMAVDMDKDLSRGEKLLNNLGGMFSKPWKPKKTKNITGPMITPDKPSKKSENHKEE 190

Query: 199 KERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSDMSDILVQLKEMAVDMGSE 258
           +E+LGL   K R+S++ A  +PTNA+QKVE EK KQDD LSD+SDIL  LK MAVDMGSE
Sbjct: 191 REKLGL-GAKGRSSSQPALDQPTNALQKVEQEKAKQDDGLSDLSDILGDLKSMAVDMGSE 250

Query: 259 MDRQNVALDHASVDVEVLGDRVKDANRRGRRLLGK 294
           +D+QN ALDH   DV+ L  RV+ AN+R R LL K
Sbjct: 251 IDKQNKALDHLGDDVDELNSRVQGANQRARHLLSK 263

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BLAST of CSPI01G05950 vs. ExPASy Swiss-Prot
Match: Q9SD96 (SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 1.8e-55
Identity = 125/259 (48.26%), Postives = 167/259 (64.48%), Query Frame = 0


Query: 35  SSQRTSSDPSLLTEKFSSNPFDDDHGHAPSSSSSTTWSERNKYKNDFRDSGGVENQSVQE 94
           SS     D     + FS NPFDDD                 ++ +  + SGG ENQ+VQE
Sbjct: 6   SSWNPFDDEKEAAKSFSLNPFDDDDDDK---------EVEKRFTSSLKPSGGKENQTVQE 65

Query: 95  LENYAVYKAEETTKAVNGCLKIAEEIREDATQTLVALHHQGEQITRTHMVAADIDYDLSR 154
           LE+YAVY +EETTK V GCLK+AEEIR DA++TLV L+ QG+QITRTH    D+D+ LSR
Sbjct: 66  LESYAVYNSEETTKTVQGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSR 125

Query: 155 GEKLLGNLGGIFSKTWKPKKTRPISGPIISSDDLVRRKGNHLEQKERLGLTHHKERTSTK 214
           GEK+LG LGG+FS+TWKPKK+R I+GP+I+  D  +RK   L+ +E+LGL    +  S  
Sbjct: 126 GEKILGRLGGVFSRTWKPKKSRSITGPVITKGDSPKRKVIDLKTREKLGLNPSLKPKS-- 185

Query: 215 AAFAEPTNAIQKVEVEKMKQDDALSDMSDILVQLKEMAVDMGSEMDRQNVALDHASVDVE 274
               E  +A QK ++   KQD+AL+D+S +L +LK MAVDMG+ ++RQ   LDH   + +
Sbjct: 186 KTLPEAVDAYQKTQI--AKQDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNAD 245

Query: 275 VLGDRVKDANRRGRRLLGK 294
            L  RVK +N+R R LL K
Sbjct: 246 ELNYRVKQSNQRARYLLRK 251

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BLAST of CSPI01G05950 vs. ExPASy Swiss-Prot
Match: Q17QQ3 (Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 5.3e-10
Identity = 54/206 (26.21%), Postives = 99/206 (48.06%), Query Frame = 0


Query: 92  VQELENYAVYKAEETTKAVNGCLKIAEEIREDATQTLVALHHQGEQITRTHMVAADIDYD 151
           ++E++  A   A+E+ ++    L++ EE ++   +TLV L  QGEQ+ R       I+ D
Sbjct: 11  LEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 152 LSRGEKLLGNLGGIFSKTWKPKKTRPISGPIISSDDLVRRKGNHLE---QKERLGLTHHK 211
           +   EK L +LG        P         + SSD   +  GN+ +     +   +   +
Sbjct: 71  MKEAEKNLTDLGKFCGLCVCP------CNKLKSSDAYKKAWGNNQDGVVASQPARVVDER 130

Query: 212 ERTSTKAAFAEPTNAIQKV--EVEKMKQDDALSDMSDILVQLKEMAVDMGSEMDRQNVAL 271
           E+ +    F      I++V  +  + + D+ L  +S I+  L+ MA+DMG+E+D QN  +
Sbjct: 131 EQMAISGGF------IRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQI 190

Query: 272 DHASVDVEVLGDRVKDANRRGRRLLG 293
           D      +    R+ +AN+R  ++LG
Sbjct: 191 DRIMEKADSNKTRIDEANQRATKMLG 204

[Close]

BLAST of CSPI01G05950 vs. ExPASy Swiss-Prot
Match: P60878 (Synaptosomal-associated protein 25 OS=Gallus gallus OX=9031 GN=SNAP25 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 5.3e-10
Identity = 54/206 (26.21%), Postives = 99/206 (48.06%), Query Frame = 0


Query: 92  VQELENYAVYKAEETTKAVNGCLKIAEEIREDATQTLVALHHQGEQITRTHMVAADIDYD 151
           ++E++  A   A+E+ ++    L++ EE ++   +TLV L  QGEQ+ R       I+ D
Sbjct: 11  LEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 152 LSRGEKLLGNLGGIFSKTWKPKKTRPISGPIISSDDLVRRKGNHLE---QKERLGLTHHK 211
           +   EK L +LG        P         + SSD   +  GN+ +     +   +   +
Sbjct: 71  MKEAEKNLTDLGKFCGLCVCP------CNKLKSSDAYKKAWGNNQDGVVASQPARVVDER 130

Query: 212 ERTSTKAAFAEPTNAIQKV--EVEKMKQDDALSDMSDILVQLKEMAVDMGSEMDRQNVAL 271
           E+ +    F      I++V  +  + + D+ L  +S I+  L+ MA+DMG+E+D QN  +
Sbjct: 131 EQMAISGGF------IRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQI 190

Query: 272 DHASVDVEVLGDRVKDANRRGRRLLG 293
           D      +    R+ +AN+R  ++LG
Sbjct: 191 DRIMEKADSNKTRIDEANQRATKMLG 204

[Close]

BLAST of CSPI01G05950 vs. ExPASy TrEMBL
Match: A0A0A0LQ81 (t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G039020 PE=4 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 7.1e-159
Identity = 293/293 (100.00%), Postives = 293/293 (100.00%), Query Frame = 0


Query: 1   MFGSRKVHFRLGKHSSTPNPIESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60
           MFGSRKVHFRLGKHSSTPNPIESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG
Sbjct: 1   MFGSRKVHFRLGKHSSTPNPIESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60

Query: 61  HAPSSSSSTTWSERNKYKNDFRDSGGVENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120
           HAPSSSSSTTWSERNKYKNDFRDSGGVENQSVQELENYAVYKAEETTKAVNGCLKIAEEI
Sbjct: 61  HAPSSSSSTTWSERNKYKNDFRDSGGVENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120

Query: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGIFSKTWKPKKTRPISG 180
           REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGIFSKTWKPKKTRPISG
Sbjct: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGIFSKTWKPKKTRPISG 180

Query: 181 PIISSDDLVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240
           PIISSDDLVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD
Sbjct: 181 PIISSDDLVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240

Query: 241 MSDILVQLKEMAVDMGSEMDRQNVALDHASVDVEVLGDRVKDANRRGRRLLGK 294
           MSDILVQLKEMAVDMGSEMDRQNVALDHASVDVEVLGDRVKDANRRGRRLLGK
Sbjct: 241 MSDILVQLKEMAVDMGSEMDRQNVALDHASVDVEVLGDRVKDANRRGRRLLGK 293

[Close]

BLAST of CSPI01G05950 vs. ExPASy TrEMBL
Match: A0A5D3BJM2 (SNAP25-like proteinous protein SNAP33-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G001190 PE=4 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 2.0e-153
Identity = 284/293 (96.93%), Postives = 289/293 (98.63%), Query Frame = 0


Query: 1   MFGSRKVHFRLGKHSSTPNPIESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60
           MFGS+KVHFR+GKHSSTPNP ESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG
Sbjct: 1   MFGSKKVHFRIGKHSSTPNPFESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60

Query: 61  HAPSSSSSTTWSERNKYKNDFRDSGGVENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120
           HAPSSSSS TWSERNKYKNDFRDSGG+ENQSVQELENYAVYKAEETTKAVNGCLKIAEEI
Sbjct: 61  HAPSSSSSITWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120

Query: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGIFSKTWKPKKTRPISG 180
           REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGG+FSKTWKPKKTRPISG
Sbjct: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180

Query: 181 PIISSDDLVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240
           PIISSD+ VRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD
Sbjct: 181 PIISSDE-VRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240

Query: 241 MSDILVQLKEMAVDMGSEMDRQNVALDHASVDVEVLGDRVKDANRRGRRLLGK 294
           MSDILVQLKEMAVDMGSEMDRQNVALD ASVDVEVLGDRVKDANRRGRRLLGK
Sbjct: 241 MSDILVQLKEMAVDMGSEMDRQNVALDRASVDVEVLGDRVKDANRRGRRLLGK 292

[Close]

BLAST of CSPI01G05950 vs. ExPASy TrEMBL
Match: A0A1S3BI33 (SNAP25 homologous protein SNAP33-like OS=Cucumis melo OX=3656 GN=LOC103490217 PE=4 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 2.0e-153
Identity = 284/293 (96.93%), Postives = 289/293 (98.63%), Query Frame = 0


Query: 1   MFGSRKVHFRLGKHSSTPNPIESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60
           MFGS+KVHFR+GKHSSTPNP ESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG
Sbjct: 1   MFGSKKVHFRIGKHSSTPNPFESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60

Query: 61  HAPSSSSSTTWSERNKYKNDFRDSGGVENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120
           HAPSSSSS TWSERNKYKNDFRDSGG+ENQSVQELENYAVYKAEETTKAVNGCLKIAEEI
Sbjct: 61  HAPSSSSSITWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120

Query: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGIFSKTWKPKKTRPISG 180
           REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGG+FSKTWKPKKTRPISG
Sbjct: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180

Query: 181 PIISSDDLVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240
           PIISSD+ VRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD
Sbjct: 181 PIISSDE-VRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240

Query: 241 MSDILVQLKEMAVDMGSEMDRQNVALDHASVDVEVLGDRVKDANRRGRRLLGK 294
           MSDILVQLKEMAVDMGSEMDRQNVALD ASVDVEVLGDRVKDANRRGRRLLGK
Sbjct: 241 MSDILVQLKEMAVDMGSEMDRQNVALDRASVDVEVLGDRVKDANRRGRRLLGK 292

[Close]

BLAST of CSPI01G05950 vs. ExPASy TrEMBL
Match: A0A6J1E891 (SNAP25 homologous protein SNAP33-like OS=Cucurbita moschata OX=3662 GN=LOC111431714 PE=4 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 1.3e-141
Identity = 258/293 (88.05%), Postives = 277/293 (94.54%), Query Frame = 0


Query: 1   MFGSRKVHFRLGKHSSTPNPIESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60
           MFGSRK H R+GKHSSTPNP ESDD VKAKQN VS+QRTSSDPSL+TE F SNPFDD HG
Sbjct: 1   MFGSRKAHLRIGKHSSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVTENFGSNPFDDHHG 60

Query: 61  HAPSSSSSTTWSERNKYKNDFRDSGGVENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120
            APSSS S TWSERNKYKNDFRDSGG+ENQSVQELENYAVYK+EETTKAVNGCLKIAEEI
Sbjct: 61  QAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI 120

Query: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGIFSKTWKPKKTRPISG 180
           R+DATQTLVALH QGEQI RTHMVAADIDYDLSRGEKLLGNLGG+FSKTWKPKKTRPISG
Sbjct: 121 RDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180

Query: 181 PIISSDDLVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240
           P+ISS+DLVRRKGNH+EQKE+LGLT HK+RT+TKAA+AEPT+AIQKVEVEK+KQDDALSD
Sbjct: 181 PVISSEDLVRRKGNHMEQKEKLGLTRHKDRTNTKAAYAEPTSAIQKVEVEKVKQDDALSD 240

Query: 241 MSDILVQLKEMAVDMGSEMDRQNVALDHASVDVEVLGDRVKDANRRGRRLLGK 294
           MSDILV+LKEMAVDMG E+DRQN ALDHASVDVEV+GDRVKDANRRGRRLLGK
Sbjct: 241 MSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGRRLLGK 293

[Close]

BLAST of CSPI01G05950 vs. ExPASy TrEMBL
Match: A0A6J1KE92 (SNAP25 homologous protein SNAP33-like OS=Cucurbita maxima OX=3661 GN=LOC111495025 PE=4 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 1.7e-141
Identity = 258/293 (88.05%), Postives = 277/293 (94.54%), Query Frame = 0


Query: 1   MFGSRKVHFRLGKHSSTPNPIESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60
           MFGSRK H R+GKH STPNP ESDD VKAKQN VS+QRTSSDPSL+ E F SNPFDDDHG
Sbjct: 1   MFGSRKGHLRIGKHGSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVAENFGSNPFDDDHG 60

Query: 61  HAPSSSSSTTWSERNKYKNDFRDSGGVENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120
           HAPSSSSS TWSERNKYKNDFRDSGG+ENQSVQELENYAVYK+EETTKAVNGCLKIAEEI
Sbjct: 61  HAPSSSSSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI 120

Query: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGIFSKTWKPKKTRPISG 180
           R+DATQTLVALH QGEQITRTH+VAADIDYDLSRGEKLLGNLGG+FSKTWKPKKTRPISG
Sbjct: 121 RDDATQTLVALHQQGEQITRTHVVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180

Query: 181 PIISSDDLVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240
           P+ISS+DLVRRKGNH+EQKE+LGLTHHK RT+TKAA+AEPT+AIQKVEVEK+KQDDALSD
Sbjct: 181 PVISSEDLVRRKGNHMEQKEKLGLTHHKGRTNTKAAYAEPTSAIQKVEVEKVKQDDALSD 240

Query: 241 MSDILVQLKEMAVDMGSEMDRQNVALDHASVDVEVLGDRVKDANRRGRRLLGK 294
           MSDILV+LKEMAVDMG E+DRQN ALDHASVDVEV+GDRVKDANRRG RLL K
Sbjct: 241 MSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGHRLLRK 293

[Close]

BLAST of CSPI01G05950 vs. NCBI nr
Match: XP_004137424.1 (SNAP25 homologous protein SNAP33 [Cucumis sativus] >XP_031743143.1 SNAP25 homologous protein SNAP33 [Cucumis sativus] >KGN64035.1 hypothetical protein Csa_013597 [Cucumis sativus])

HSP 1 Score: 569.7 bits (1467), Expect = 1.5e-158
Identity = 293/293 (100.00%), Postives = 293/293 (100.00%), Query Frame = 0


Query: 1   MFGSRKVHFRLGKHSSTPNPIESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60
           MFGSRKVHFRLGKHSSTPNPIESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG
Sbjct: 1   MFGSRKVHFRLGKHSSTPNPIESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60

Query: 61  HAPSSSSSTTWSERNKYKNDFRDSGGVENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120
           HAPSSSSSTTWSERNKYKNDFRDSGGVENQSVQELENYAVYKAEETTKAVNGCLKIAEEI
Sbjct: 61  HAPSSSSSTTWSERNKYKNDFRDSGGVENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120

Query: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGIFSKTWKPKKTRPISG 180
           REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGIFSKTWKPKKTRPISG
Sbjct: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGIFSKTWKPKKTRPISG 180

Query: 181 PIISSDDLVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240
           PIISSDDLVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD
Sbjct: 181 PIISSDDLVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240

Query: 241 MSDILVQLKEMAVDMGSEMDRQNVALDHASVDVEVLGDRVKDANRRGRRLLGK 294
           MSDILVQLKEMAVDMGSEMDRQNVALDHASVDVEVLGDRVKDANRRGRRLLGK
Sbjct: 241 MSDILVQLKEMAVDMGSEMDRQNVALDHASVDVEVLGDRVKDANRRGRRLLGK 293

[Close]

BLAST of CSPI01G05950 vs. NCBI nr
Match: XP_008447972.1 (PREDICTED: SNAP25 homologous protein SNAP33-like [Cucumis melo] >TYJ98921.1 SNAP25-like proteinous protein SNAP33-like [Cucumis melo var. makuwa])

HSP 1 Score: 551.6 bits (1420), Expect = 4.1e-153
Identity = 284/293 (96.93%), Postives = 289/293 (98.63%), Query Frame = 0


Query: 1   MFGSRKVHFRLGKHSSTPNPIESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60
           MFGS+KVHFR+GKHSSTPNP ESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG
Sbjct: 1   MFGSKKVHFRIGKHSSTPNPFESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60

Query: 61  HAPSSSSSTTWSERNKYKNDFRDSGGVENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120
           HAPSSSSS TWSERNKYKNDFRDSGG+ENQSVQELENYAVYKAEETTKAVNGCLKIAEEI
Sbjct: 61  HAPSSSSSITWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120

Query: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGIFSKTWKPKKTRPISG 180
           REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGG+FSKTWKPKKTRPISG
Sbjct: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180

Query: 181 PIISSDDLVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240
           PIISSD+ VRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD
Sbjct: 181 PIISSDE-VRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240

Query: 241 MSDILVQLKEMAVDMGSEMDRQNVALDHASVDVEVLGDRVKDANRRGRRLLGK 294
           MSDILVQLKEMAVDMGSEMDRQNVALD ASVDVEVLGDRVKDANRRGRRLLGK
Sbjct: 241 MSDILVQLKEMAVDMGSEMDRQNVALDRASVDVEVLGDRVKDANRRGRRLLGK 292

[Close]

BLAST of CSPI01G05950 vs. NCBI nr
Match: XP_038894894.1 (SNAP25 homologous protein SNAP33-like [Benincasa hispida] >XP_038894895.1 SNAP25 homologous protein SNAP33-like [Benincasa hispida])

HSP 1 Score: 526.2 bits (1354), Expect = 1.9e-145
Identity = 268/293 (91.47%), Postives = 279/293 (95.22%), Query Frame = 0


Query: 1   MFGSRKVHFRLGKHSSTPNPIESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60
           MFGSRK HFR+GKHSSTPNP ESDD VKA+QN VSSQRTSSDPSLLTE FSSNPFDDDHG
Sbjct: 1   MFGSRKAHFRIGKHSSTPNPFESDDGVKAQQNQVSSQRTSSDPSLLTENFSSNPFDDDHG 60

Query: 61  HAPSSSSSTTWSERNKYKNDFRDSGGVENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120
           HAPSSSSS TWSERNKYKNDFRDSGG+ENQSVQELENYAVYKAEETTKAVNGCLKIAEEI
Sbjct: 61  HAPSSSSSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120

Query: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGIFSKTWKPKKTRPISG 180
           REDATQTLV LHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGG+FSKTWKPKKTRPISG
Sbjct: 121 REDATQTLVTLHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180

Query: 181 PIISSDDLVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240
           PII+SDDLVRRKGNHLEQKERLGLTHHK RT+TK AFAEPT+AIQKVEVEK+KQDDALSD
Sbjct: 181 PIINSDDLVRRKGNHLEQKERLGLTHHKGRTNTKTAFAEPTSAIQKVEVEKVKQDDALSD 240

Query: 241 MSDILVQLKEMAVDMGSEMDRQNVALDHASVDVEVLGDRVKDANRRGRRLLGK 294
           MSDILV+LKEMAVDMG E+DRQN  LD AS DVEVLGDRVKDANRRGRRLLG+
Sbjct: 241 MSDILVELKEMAVDMGCEIDRQNNVLDRASDDVEVLGDRVKDANRRGRRLLGR 293

[Close]

BLAST of CSPI01G05950 vs. NCBI nr
Match: XP_023520322.1 (SNAP25 homologous protein SNAP33-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 515.4 bits (1326), Expect = 3.3e-142
Identity = 259/293 (88.40%), Postives = 278/293 (94.88%), Query Frame = 0


Query: 1   MFGSRKVHFRLGKHSSTPNPIESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60
           MFGSRK H R+GKHSSTPNP ESDD VKAKQN VS+QRTSSDPSL+TE F SNPFDDDHG
Sbjct: 1   MFGSRKAHLRIGKHSSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVTENFGSNPFDDDHG 60

Query: 61  HAPSSSSSTTWSERNKYKNDFRDSGGVENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120
           HAPSSS S TWSERNKYKNDFRDSGG+ENQSVQELENYAVYK+EETTKAVNGCLKIAEEI
Sbjct: 61  HAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI 120

Query: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGIFSKTWKPKKTRPISG 180
           R+DATQTLVALH QGEQI RTHMVAADIDYDLSRGEKLLGNLGG+FSKTWKPKKTRPISG
Sbjct: 121 RDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180

Query: 181 PIISSDDLVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240
           P+ISS+DLVRRKGNH+EQKE+LGLT HK+RT+TKAA+AEPT+AIQK EVEK+KQDDALSD
Sbjct: 181 PVISSEDLVRRKGNHMEQKEKLGLTRHKDRTNTKAAYAEPTSAIQKDEVEKVKQDDALSD 240

Query: 241 MSDILVQLKEMAVDMGSEMDRQNVALDHASVDVEVLGDRVKDANRRGRRLLGK 294
           MSDILV+LKEMAVDMG E+DRQN ALDHASVDVEV+GDRVKDANRRGRRLLGK
Sbjct: 241 MSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGRRLLGK 293

[Close]

BLAST of CSPI01G05950 vs. NCBI nr
Match: XP_023523341.1 (SNAP25 homologous protein SNAP33-like [Cucurbita pepo subsp. pepo] >XP_023523342.1 SNAP25 homologous protein SNAP33-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 515.4 bits (1326), Expect = 3.3e-142
Identity = 262/293 (89.42%), Postives = 278/293 (94.88%), Query Frame = 0


Query: 1   MFGSRKVHFRLGKHSSTPNPIESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60
           MFGSRK H ++G+HSSTPNP ESDD  KAKQNLV+SQRTSSDPSL+ E F SNPFDDDHG
Sbjct: 1   MFGSRKAHLKIGRHSSTPNPFESDDGFKAKQNLVTSQRTSSDPSLVKESFISNPFDDDHG 60

Query: 61  HAPSSSSSTTWSERNKYKNDFRDSGGVENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120
           HAPSSSSS TWSERNKYKNDFRDSGG+ENQSVQELENYA YKAEETTKAVNGCLKIAEEI
Sbjct: 61  HAPSSSSSLTWSERNKYKNDFRDSGGLENQSVQELENYATYKAEETTKAVNGCLKIAEEI 120

Query: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGIFSKTWKPKKTRPISG 180
           REDATQTLVALH QGEQITRTHMVAADIDYDLSRGEKLLGNLGGIFSKTWKPKKTRPISG
Sbjct: 121 REDATQTLVALHQQGEQITRTHMVAADIDYDLSRGEKLLGNLGGIFSKTWKPKKTRPISG 180

Query: 181 PIISSDDLVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240
           PI++SDDLVRRKG+HLEQKE+LGLTHHK RT+TKAAFAEPTNAIQKVEVEK+KQDDALSD
Sbjct: 181 PIVNSDDLVRRKGSHLEQKEKLGLTHHKGRTNTKAAFAEPTNAIQKVEVEKVKQDDALSD 240

Query: 241 MSDILVQLKEMAVDMGSEMDRQNVALDHASVDVEVLGDRVKDANRRGRRLLGK 294
           MS+IL +LKEMAVDMG E+DRQ VALDHASVDVEVLGDRVKDA+RRGRRLLGK
Sbjct: 241 MSNILEELKEMAVDMGCEIDRQIVALDHASVDVEVLGDRVKDASRRGRRLLGK 293

[Close]

BLAST of CSPI01G05950 vs. TAIR 10
Match: AT5G61210.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 33 )

HSP 1 Score: 319.3 bits (817), Expect = 3.2e-87
Identity = 177/303 (58.42%), Postives = 223/303 (73.60%), Query Frame = 0


Query: 1   MFGSRKVHFRLGKH------SSTPNPIESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNP 60
           MFG RK    L KH      SS PNP +SDD+   K  L  S+RT+S+PSL      +NP
Sbjct: 1   MFGLRKSPANLPKHNSVDLKSSKPNPFDSDDESDNKHTLNPSKRTTSEPSLAD---MTNP 60

Query: 61  FDDDH---GHAPSSSSSTTWSERNKYKNDFRDSGGVENQSVQELENYAVYKAEETTKAVN 120
           F  +    G + SS  S   + + +YKN+FRDSGG+ENQSVQELE YAVYKAEETTK+V 
Sbjct: 61  FGGERVQKGDSSSSKQSLFSNSKYQYKNNFRDSGGIENQSVQELEGYAVYKAEETTKSVQ 120

Query: 121 GCLKIAEEIREDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGIFSKTWK 180
           GCLK+AE+IR DAT+TLV LH QGEQITRTH  A +ID+DLSRGEKLLG+LGG+FSKTWK
Sbjct: 121 GCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGGMFSKTWK 180

Query: 181 PKKTRPISGPIISSDDLVRRKGNHLEQKERLGL-THHKERTSTKAAFAEPTNAIQKVEVE 240
           PKKTRPI+GP+++ DD   R+ NHLE++E+LGL +  + ++ T+    E  +A Q+VE+E
Sbjct: 181 PKKTRPINGPVVTRDDSPTRRVNHLEKREKLGLNSAPRGQSRTREPLPESADAYQRVEME 240

Query: 241 KMKQDDALSDMSDILVQLKEMAVDMGSEMDRQNVALDHASVDVEVLGDRVKDANRRGRRL 294
           K KQDD LSD+SDIL +LK MAVDMGSE+++QN  LDH   DV+ L  RV+ +N+RGRRL
Sbjct: 241 KAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQSNQRGRRL 300

[Close]

BLAST of CSPI01G05950 vs. TAIR 10
Match: AT1G13890.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 30 )

HSP 1 Score: 270.4 bits (690), Expect = 1.7e-72
Identity = 150/275 (54.55%), Postives = 189/275 (68.73%), Query Frame = 0


Query: 19  NPIESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHGHAPSSSSSTTWSERNKYK 78
           N + ++        + + +RTSS+P L+T       FDDD                +KYK
Sbjct: 11  NKLPNESSNNKGGTITAGRRTSSEPILITP-----DFDDD----------------DKYK 70

Query: 79  NDFRDSGGVENQSVQELENYAVYKAEETTKAVNGCLKIAEEIREDATQTLVALHHQGEQI 138
           N F DSGG+++Q+ +ELE YAVYKAEETTK VN CLKIAE+IR D  +TL  LH QGEQI
Sbjct: 71  NGFNDSGGLQSQTTEELEKYAVYKAEETTKGVNNCLKIAEDIRSDGARTLEMLHQQGEQI 130

Query: 139 TRTHMVAADIDYDLSRGEKLLGNLGGIFSKTWKPKKTRPISGPIISSDDLVRRKGNHLEQ 198
            RTH +A D+D DLSRGEKLL NLGG+FSK WKPKKT+ I+GP+I+ D   ++  NH E+
Sbjct: 131 NRTHEMAVDMDKDLSRGEKLLNNLGGMFSKPWKPKKTKNITGPMITPDKPSKKSENHKEE 190

Query: 199 KERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSDMSDILVQLKEMAVDMGSE 258
           +E+LGL   K R+S++ A  +PTNA+QKVE EK KQDD LSD+SDIL  LK MAVDMGSE
Sbjct: 191 REKLGL-GAKGRSSSQPALDQPTNALQKVEQEKAKQDDGLSDLSDILGDLKSMAVDMGSE 250

Query: 259 MDRQNVALDHASVDVEVLGDRVKDANRRGRRLLGK 294
           +D+QN ALDH   DV+ L  RV+ AN+R R LL K
Sbjct: 251 IDKQNKALDHLGDDVDELNSRVQGANQRARHLLSK 263

[Close]

BLAST of CSPI01G05950 vs. TAIR 10
Match: AT5G07880.1 (synaptosomal-associated protein SNAP25-like 29 )

HSP 1 Score: 217.6 bits (553), Expect = 1.3e-56
Identity = 125/259 (48.26%), Postives = 167/259 (64.48%), Query Frame = 0


Query: 35  SSQRTSSDPSLLTEKFSSNPFDDDHGHAPSSSSSTTWSERNKYKNDFRDSGGVENQSVQE 94
           SS     D     + FS NPFDDD                 ++ +  + SGG ENQ+VQE
Sbjct: 6   SSWNPFDDEKEAAKSFSLNPFDDDDDDK---------EVEKRFTSSLKPSGGKENQTVQE 65

Query: 95  LENYAVYKAEETTKAVNGCLKIAEEIREDATQTLVALHHQGEQITRTHMVAADIDYDLSR 154
           LE+YAVY +EETTK V GCLK+AEEIR DA++TLV L+ QG+QITRTH    D+D+ LSR
Sbjct: 66  LESYAVYNSEETTKTVQGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSR 125

Query: 155 GEKLLGNLGGIFSKTWKPKKTRPISGPIISSDDLVRRKGNHLEQKERLGLTHHKERTSTK 214
           GEK+LG LGG+FS+TWKPKK+R I+GP+I+  D  +RK   L+ +E+LGL    +  S  
Sbjct: 126 GEKILGRLGGVFSRTWKPKKSRSITGPVITKGDSPKRKVIDLKTREKLGLNPSLKPKS-- 185

Query: 215 AAFAEPTNAIQKVEVEKMKQDDALSDMSDILVQLKEMAVDMGSEMDRQNVALDHASVDVE 274
               E  +A QK ++   KQD+AL+D+S +L +LK MAVDMG+ ++RQ   LDH   + +
Sbjct: 186 KTLPEAVDAYQKTQI--AKQDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNAD 245

Query: 275 VLGDRVKDANRRGRRLLGK 294
            L  RVK +N+R R LL K
Sbjct: 246 ELNYRVKQSNQRARYLLRK 251

The following BLAST results are available for this feature:

BLAST of CSPI01G05950 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9S7P9	4.5e-86	58.42	SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 ...	[more]
Q9LMG8	2.4e-71	54.55	Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNA...	[more]
Q9SD96	1.8e-55	48.26	SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 ...	[more]
Q17QQ3	5.3e-10	26.21	Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1	[more]
P60878	5.3e-10	26.21	Synaptosomal-associated protein 25 OS=Gallus gallus OX=9031 GN=SNAP25 PE=1 SV=1	[more]

BLAST of CSPI01G05950 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LQ81	7.1e-159	100.00	t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=365...	[more]
A0A5D3BJM2	2.0e-153	96.93	SNAP25-like proteinous protein SNAP33-like OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3BI33	2.0e-153	96.93	SNAP25 homologous protein SNAP33-like OS=Cucumis melo OX=3656 GN=LOC103490217 PE...	[more]
A0A6J1E891	1.3e-141	88.05	SNAP25 homologous protein SNAP33-like OS=Cucurbita moschata OX=3662 GN=LOC111431...	[more]
A0A6J1KE92	1.7e-141	88.05	SNAP25 homologous protein SNAP33-like OS=Cucurbita maxima OX=3661 GN=LOC11149502...	[more]

BLAST of CSPI01G05950 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004137424.1	1.5e-158	100.00	SNAP25 homologous protein SNAP33 [Cucumis sativus] >XP_031743143.1 SNAP25 homolo...	[more]
XP_008447972.1	4.1e-153	96.93	PREDICTED: SNAP25 homologous protein SNAP33-like [Cucumis melo] >TYJ98921.1 SNAP...	[more]
XP_038894894.1	1.9e-145	91.47	SNAP25 homologous protein SNAP33-like [Benincasa hispida] >XP_038894895.1 SNAP25...	[more]
XP_023520322.1	3.3e-142	88.40	SNAP25 homologous protein SNAP33-like [Cucurbita pepo subsp. pepo]	[more]
XP_023523341.1	3.3e-142	89.42	SNAP25 homologous protein SNAP33-like [Cucurbita pepo subsp. pepo] >XP_023523342...	[more]

BLAST of CSPI01G05950 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G61210.1	3.2e-87	58.42	soluble N-ethylmaleimide-sensitive factor adaptor protein 33	[more]
AT1G13890.1	1.7e-72	54.55	soluble N-ethylmaleimide-sensitive factor adaptor protein 30	[more]
AT5G07880.1	1.3e-56	48.26	synaptosomal-associated protein SNAP25-like 29	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.20.5.110		coord: 86..163 e-value: 1.3E-19 score: 72.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..88
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 58..74
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 32..51
None	No IPR available	PANTHER	PTHR19305:SF30	TARGET SNARE COILED-COIL DOMAIN-CONTAINING PROTEIN-RELATED	coord: 1..229
None	No IPR available	PANTHER	PTHR19305:SF31	SNAP25-LIKE PROTEINOUS PROTEIN SNAP33	coord: 14..229
None	No IPR available	PANTHER	PTHR19305	SYNAPTOSOMAL ASSOCIATED PROTEIN	coord: 1..229 coord: 14..229
None	No IPR available	SUPERFAMILY	58038	SNARE fusion complex	coord: 90..160
IPR044766	NPSN/SNAP25-like, N-terminal SNARE domain	CDD	cd15861	SNARE_SNAP25N_23N_29N_SEC9N	coord: 97..161 e-value: 1.2869E-21 score: 83.0063

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G05950.3	CSPI01G05950.3	mRNA
CSPI01G05950.2	CSPI01G05950.2	mRNA
CSPI01G05950.1	CSPI01G05950.1	mRNA
CSPI01G05950.4	CSPI01G05950.4	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006886	intracellular protein transport
biological_process	GO:0061025	membrane fusion
biological_process	GO:0015031	protein transport
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0031201	SNARE complex
molecular_function	GO:0005484	SNAP receptor activity