CSPI01G04290 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G04290
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Calcium-binding EF-hand family protein
Location	Chr1: 2676176 .. 2677438 (-)
RNA-Seq Expression	CSPI01G04290
Synteny	CSPI01G04290

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTGAAAAAGAGAAAAATGAGGAGAAATTGTGAGCCGTCGGATCTTCTCCCCTCATTATTCTTCAATATTTCTCCCTATAAATACCCAATTTTCCGATCCTATTTCTTCATTACAAAAATCGAAGTTTAACCCATTCCGTTTTCCTCTCTATCAAGTTTTGACCTCCAAAAATGGCCACCGACCCCAACCCGATTCCCACCTCAAAATCCGAAGCCGACCAAATCGCCAAGCAGAACCCATCCGTCTTACTCCAGGACGACGAAGAACTCCGCAAGGTTTTCGAACGCTTCGATGCGAATGGCGACGGTAAGATCTCCATCTCCGAGCTCGATGCCGTTCTCACTTCCTTAACGTTAAAATCCGCCATCCCCTTAGAAGAGCTTCGATCTGTCATGGACGATCTCGACTCTGATAAGGACGGGTATATCAACATCGATGAATTCGCCGCCTTCTGTAAGAAACCGATGGCCTCCGACGAAGCTGGAGCAGCGGAGCTTCGTGACGCTTTCGACCTCTACGATCAGGACCGTAACGGCCTTATTTCTCAGTCCGAGCTCCATCTCGTTCTCAATCGCCTCGGAATCAGTTGTTCTAAGGAAGACTGTCAAAAAATGATCAACTCCGTTGATTCGGATGGAGACGGAAATGTGAATTTCGAAGAGTTTAGGAAGATGATGACGGATAATTCCAAATCGAAGGCCGCTCAACAGAACGGAACTGCCGCGGCGGCACCCTAGCCCTAACTCTAGGTTTTGGTAAGTTGTTTAGTAATTAGCCGCGTGAGAGAGAGGTAACGAAGTCGATTTCTATCCTTCGTTCCTTTTCCATTTCGGTTTCTTTTTTCTTCTTTTTAAAATTTCTCTGTGTTCTATCCTTAATCTGAATCCAATCCAACTGTTTTTGTTTTCTTATTTTGTCGAAATGTAACAAAAAAAAAAAAAATCGGATGTGCTTCTGTTGATCATCCGCCATTAATGCAGATCTTTAATTTAAACTCTTTCTACAATCCGATTGAAGATTGAAGAGAAACTTTTCCGGAAAATCGATTGGTATTTCCGGATTCATAGTGTGCATTGCTGTTGAGTGCTGAGTACATTTCATCCCTATTTTTGTTTCTTTTTTTCCGTAAAATTAGAACACTAATAAATGAAATTAAACAAAATAAAAAATAGAGTGGGATTTTTTTTTCGAATATAAAAAGGGTAAAATTGAATATTCCAAACTTATCCCGTGATCTTAAACTTGTCACTATTTTATTGGGC

mRNA sequence

ATTGAAAAAGAGAAAAATGAGGAGAAATTGTGAGCCGTCGGATCTTCTCCCCTCATTATTCTTCAATATTTCTCCCTATAAATACCCAATTTTCCGATCCTATTTCTTCATTACAAAAATCGAAGTTTAACCCATTCCGTTTTCCTCTCTATCAAGTTTTGACCTCCAAAAATGGCCACCGACCCCAACCCGATTCCCACCTCAAAATCCGAAGCCGACCAAATCGCCAAGCAGAACCCATCCGTCTTACTCCAGGACGACGAAGAACTCCGCAAGGTTTTCGAACGCTTCGATGCGAATGGCGACGGTAAGATCTCCATCTCCGAGCTCGATGCCGTTCTCACTTCCTTAACGTTAAAATCCGCCATCCCCTTAGAAGAGCTTCGATCTGTCATGGACGATCTCGACTCTGATAAGGACGGGTATATCAACATCGATGAATTCGCCGCCTTCTGTAAGAAACCGATGGCCTCCGACGAAGCTGGAGCAGCGGAGCTTCGTGACGCTTTCGACCTCTACGATCAGGACCGTAACGGCCTTATTTCTCAGTCCGAGCTCCATCTCGTTCTCAATCGCCTCGGAATCAGTTGTTCTAAGGAAGACTGTCAAAAAATGATCAACTCCGTTGATTCGGATGGAGACGGAAATGTGAATTTCGAAGAGTTTAGGAAGATGATGACGGATAATTCCAAATCGAAGGCCGCTCAACAGAACGGAACTGCCGCGGCGGCACCCTAGCCCTAACTCTAGGTTTTGGTAAGTTGTTTAGTAATTAGCCGCGTGAGAGAGAGGTAACGAAGTCGATTTCTATCCTTCGTTCCTTTTCCATTTCGGTTTCTTTTTTCTTCTTTTTAAAATTTCTCTGTGTTCTATCCTTAATCTGAATCCAATCCAACTGTTTTTGTTTTCTTATTTTGTCGAAATGTAACAAAAAAAAAAAAAATCGGATGTGCTTCTGTTGATCATCCGCCATTAATGCAGATCTTTAATTTAAACTCTTTCTACAATCCGATTGAAGATTGAAGAGAAACTTTTCCGGAAAATCGATTGGTATTTCCGGATTCATAGTGTGCATTGCTGTTGAGTGCTGAGTACATTTCATCCCTATTTTTGTTTCTTTTTTTCCGTAAAATTAGAACACTAATAAATGAAATTAAACAAAATAAAAAATAGAGTGGGATTTTTTTTTCGAATATAAAAAGGGTAAAATTGAATATTCCAAACTTATCCCGTGATCTTAAACTTGTCACTATTTTATTGGGC

Coding sequence (CDS)

ATGGCCACCGACCCCAACCCGATTCCCACCTCAAAATCCGAAGCCGACCAAATCGCCAAGCAGAACCCATCCGTCTTACTCCAGGACGACGAAGAACTCCGCAAGGTTTTCGAACGCTTCGATGCGAATGGCGACGGTAAGATCTCCATCTCCGAGCTCGATGCCGTTCTCACTTCCTTAACGTTAAAATCCGCCATCCCCTTAGAAGAGCTTCGATCTGTCATGGACGATCTCGACTCTGATAAGGACGGGTATATCAACATCGATGAATTCGCCGCCTTCTGTAAGAAACCGATGGCCTCCGACGAAGCTGGAGCAGCGGAGCTTCGTGACGCTTTCGACCTCTACGATCAGGACCGTAACGGCCTTATTTCTCAGTCCGAGCTCCATCTCGTTCTCAATCGCCTCGGAATCAGTTGTTCTAAGGAAGACTGTCAAAAAATGATCAACTCCGTTGATTCGGATGGAGACGGAAATGTGAATTTCGAAGAGTTTAGGAAGATGATGACGGATAATTCCAAATCGAAGGCCGCTCAACAGAACGGAACTGCCGCGGCGGCACCCTAG

Protein sequence

MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSLTLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQNGTAAAAP*

Homology

[Close]

BLAST of CSPI01G04290 vs. ExPASy Swiss-Prot
Match: Q9LE22 (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 PE=1 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 3.0e-43
Identity = 93/170 (54.71%), Postives = 125/170 (73.53%), Query Frame = 0


Query: 17  QIAKQNPSVL-LQDDEELRKVFERFDANGDGKISISELDAVLTSLTLKSAIPLEELRSVM 76
           + AK  P+ + + + EEL+KVF++FD+NGDGKIS+ EL  V  +  + ++    EL  V+
Sbjct: 7   ETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKA--MGTSYTETELNRVL 66

Query: 77  DDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNR 136
           +++D+D+DGYIN+DEF+  C+       + AAE+RDAFDLYDQD+NGLIS SELH VLNR
Sbjct: 67  EEVDTDRDGYINLDEFSTLCR-----SSSSAAEIRDAFDLYDQDKNGLISASELHQVLNR 126

Query: 137 LGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQNGTAA 186
           LG+SCS EDC +MI  VD+DGDGNVNFEEF+KMMT +S       NG+AA
Sbjct: 127 LGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS---LLNSNGSAA 166

[Close]

BLAST of CSPI01G04290 vs. ExPASy Swiss-Prot
Match: Q9C9U8 (Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 7.0e-40
Identity = 87/160 (54.37%), Postives = 112/160 (70.00%), Query Frame = 0


Query: 22  NPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSLTLKSAIPLEELRSVMDDLDSD 81
           N S     D EL+KVF++FDANGDGKIS+SEL  V  S  + ++   EEL  V+D++D D
Sbjct: 10  NKSTTPSTDMELKKVFDKFDANGDGKISVSELGNVFKS--MGTSYTEEELNRVLDEIDID 69

Query: 82  KDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGISCS 141
            DG+IN +EFA  C+       + A E+R+AFDLYDQ++NGLIS SE+H VLNRLG++CS
Sbjct: 70  CDGFINQEEFATICR-----SSSSAVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCS 129

Query: 142 KEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQN 182
            EDC +MI  VD+DGDGNVNFEEF+KMM+     K    N
Sbjct: 130 VEDCVRMIGHVDTDGDGNVNFEEFQKMMSSPELVKGTVAN 162

[Close]

BLAST of CSPI01G04290 vs. ExPASy Swiss-Prot
Match: Q9M7R0 (Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 1.9e-37
Identity = 87/154 (56.49%), Postives = 109/154 (70.78%), Query Frame = 0


Query: 23  PSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSLTLKSAIPLEELRSVMDDLDSDK 82
           PSV LQ+  E++ VF RFDANGDGKIS  EL  VL +  L S    EE+  +M+++D+DK
Sbjct: 11  PSVYLQEPNEVQGVFNRFDANGDGKISGDELAGVLKA--LGSNTSKEEIGRIMEEIDTDK 70

Query: 83  DGYINIDEFAAFCK---KPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGIS 142
           DG+IN+ EFAAF K    P  S   G  EL++AF+LYDQD NGLIS  ELH +L RLG  
Sbjct: 71  DGFINVQEFAAFVKAETDPYPS-SGGENELKEAFELYDQDHNGLISSVELHKILTRLGER 130

Query: 143 CSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNS 174
            ++ DC +MI SVDSDGDG V+FEEF+KMMT+ S
Sbjct: 131 YAEHDCVEMIKSVDSDGDGYVSFEEFKKMMTNKS 161

[Close]

BLAST of CSPI01G04290 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 6.3e-33
Identity = 74/146 (50.68%), Postives = 104/146 (71.23%), Query Frame = 0


Query: 31  EELRKVFERFDANGDGKISISELDAVLTSLTLKSAIPLEELRSVMDDLDSDKDGYINIDE 90
           E+++KVF+RFD N DGKISI EL  V+ +L+  ++   EE +++M + D D +G+I++DE
Sbjct: 14  EDIKKVFQRFDKNNDGKISIDELKDVIGALSPNAS--QEETKAMMKEFDLDGNGFIDLDE 73

Query: 91  FAA-FCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGISCSKEDCQKMI 150
           F A F     +S+ +   +L++AFDLYD DRNG IS +ELH V+  LG  CS +DCQ+MI
Sbjct: 74  FVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMI 133

Query: 151 NSVDSDGDGNVNFEEFRKMMTDNSKS 176
           N VDSDGDG V+FEEF+KMM  N  +
Sbjct: 134 NKVDSDGDGCVDFEEFKKMMMINGSA 157

[Close]

BLAST of CSPI01G04290 vs. ExPASy Swiss-Prot
Match: Q0DJV6 (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 3.1e-32
Identity = 76/149 (51.01%), Postives = 100/149 (67.11%), Query Frame = 0


Query: 32  ELRKVFERFDANGDGKISISELDAVLTSLTLKSAIPLEELRSVMDDLDSDKDGYINIDEF 91
           EL +VF R+DANGDGKIS  EL +VL +  L + +   E+R +MD++DSD+DG++++ EF
Sbjct: 5   ELEQVFRRYDANGDGKISAEELASVLRA--LGAPLGPGEVRRMMDEMDSDRDGFVDLSEF 64

Query: 92  AAFCKKPMAS-----------DEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGISC 151
           AAF   P  +           + A  AELR+AF +YD D NG IS  ELH VL +LG  C
Sbjct: 65  AAFHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKC 124

Query: 152 SKEDCQKMINSVDSDGDGNVNFEEFRKMM 170
           S  DC +MI SVD+DGDG VNF+EF+KMM
Sbjct: 125 SVADCSRMIRSVDADGDGCVNFDEFKKMM 151

[Close]

BLAST of CSPI01G04290 vs. ExPASy TrEMBL
Match: A0A0A0LPP8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G025020 PE=4 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 2.9e-97
Identity = 188/188 (100.00%), Postives = 188/188 (100.00%), Query Frame = 0


Query: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60
           MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL
Sbjct: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
           TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180
           NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180

Query: 181 NGTAAAAP 189
           NGTAAAAP
Sbjct: 181 NGTAAAAP 188

[Close]

BLAST of CSPI01G04290 vs. ExPASy TrEMBL
Match: A0A5D3DXJ8 (Putative calcium-binding protein CML27 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G001210 PE=4 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 7.4e-93
Identity = 179/188 (95.21%), Postives = 186/188 (98.94%), Query Frame = 0


Query: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60
           MATDPN IPTS+S+ADQIAKQ PSVLL+DD+ELRKVFERFDANGDGKISI+ELDAVLTSL
Sbjct: 1   MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
           TLKSAIPLEELRSVMDDLDSDKDGYINI+EFAAFCKKPMASDEAGAAELRDAFDLYDQDR
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180
           NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAA+Q
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQ 180

Query: 181 NGTAAAAP 189
           NGTAAAAP
Sbjct: 181 NGTAAAAP 188

[Close]

BLAST of CSPI01G04290 vs. ExPASy TrEMBL
Match: A0A1S3BX51 (probable calcium-binding protein CML27 OS=Cucumis melo OX=3656 GN=LOC103494178 PE=4 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 7.4e-93
Identity = 179/188 (95.21%), Postives = 186/188 (98.94%), Query Frame = 0


Query: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60
           MATDPN IPTS+S+ADQIAKQ PSVLL+DD+ELRKVFERFDANGDGKISI+ELDAVLTSL
Sbjct: 1   MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
           TLKSAIPLEELRSVMDDLDSDKDGYINI+EFAAFCKKPMASDEAGAAELRDAFDLYDQDR
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180
           NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAA+Q
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQ 180

Query: 181 NGTAAAAP 189
           NGTAAAAP
Sbjct: 181 NGTAAAAP 188

[Close]

BLAST of CSPI01G04290 vs. ExPASy TrEMBL
Match: A0A6J1F164 (probable calcium-binding protein CML27 OS=Cucurbita moschata OX=3662 GN=LOC111441185 PE=4 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 1.4e-72
Identity = 142/188 (75.53%), Postives = 166/188 (88.30%), Query Frame = 0


Query: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60
           MATD N I  S ++ +Q+ K  PS LLQD+ ELRKVF+RFDANGDGKIS+SELD+VL S 
Sbjct: 1   MATDSNQITASNADTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKS- 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
            L S++P +ELR+VM+DLD+DKDG+INIDEFA FCK+PMAS+EAGA+ELRDAFDLYDQD 
Sbjct: 61  -LGSSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDH 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180
           NGLISQSELHLVLNRLGI+CSKEDCQ+MI+SVDSDGDGNVNFEEFRKMM++NS S+A  Q
Sbjct: 121 NGLISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEAPNQ 180

Query: 181 NGTAAAAP 189
           NGTAA AP
Sbjct: 181 NGTAATAP 186

[Close]

BLAST of CSPI01G04290 vs. ExPASy TrEMBL
Match: A0A6J1E0A3 (probable calcium-binding protein CML27 OS=Cucurbita moschata OX=3662 GN=LOC111429663 PE=4 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 1.2e-71
Identity = 145/188 (77.13%), Postives = 164/188 (87.23%), Query Frame = 0


Query: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60
           MATD NPI +S S+ADQIAK  PS LL ++EELRKVF+RFD+NGDG+ISI+ELD+VL S 
Sbjct: 1   MATDSNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKS- 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
            L SAIP EELR VM+DLD DKDG+INIDEFAAFCK+PMASDEAG +ELRDAFDLYDQD 
Sbjct: 61  -LGSAIPYEELRGVMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDH 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180
           NGLIS SELHLVL RLGI+ S EDCQ+MI++VDSDGDGNVNFEEFRKMM+D +KSKA  Q
Sbjct: 121 NGLISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPNQ 180

Query: 181 NGTAAAAP 189
           NGTAAA P
Sbjct: 181 NGTAAAGP 186

[Close]

BLAST of CSPI01G04290 vs. NCBI nr
Match: XP_004137340.1 (probable calcium-binding protein CML27 [Cucumis sativus] >KGN63870.1 hypothetical protein Csa_013908 [Cucumis sativus])

HSP 1 Score: 364.4 bits (934), Expect = 6.0e-97
Identity = 188/188 (100.00%), Postives = 188/188 (100.00%), Query Frame = 0


Query: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60
           MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL
Sbjct: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
           TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180
           NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180

Query: 181 NGTAAAAP 189
           NGTAAAAP
Sbjct: 181 NGTAAAAP 188

[Close]

BLAST of CSPI01G04290 vs. NCBI nr
Match: XP_008453475.1 (PREDICTED: probable calcium-binding protein CML27 [Cucumis melo] >KAA0058132.1 putative calcium-binding protein CML27 [Cucumis melo var. makuwa] >TYK28486.1 putative calcium-binding protein CML27 [Cucumis melo var. makuwa])

HSP 1 Score: 349.7 bits (896), Expect = 1.5e-92
Identity = 179/188 (95.21%), Postives = 186/188 (98.94%), Query Frame = 0


Query: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60
           MATDPN IPTS+S+ADQIAKQ PSVLL+DD+ELRKVFERFDANGDGKISI+ELDAVLTSL
Sbjct: 1   MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
           TLKSAIPLEELRSVMDDLDSDKDGYINI+EFAAFCKKPMASDEAGAAELRDAFDLYDQDR
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180
           NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAA+Q
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQ 180

Query: 181 NGTAAAAP 189
           NGTAAAAP
Sbjct: 181 NGTAAAAP 188

[Close]

BLAST of CSPI01G04290 vs. NCBI nr
Match: XP_038878467.1 (probable calcium-binding protein CML27 [Benincasa hispida])

HSP 1 Score: 311.2 bits (796), Expect = 6.0e-81
Identity = 161/188 (85.64%), Postives = 172/188 (91.49%), Query Frame = 0


Query: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60
           MATDPN IP S S+ADQ++K  PSV LQD+EELRKVFERFDANGDGKISISELD+VL S 
Sbjct: 1   MATDPNQIPASNSDADQVSKPKPSVFLQDNEELRKVFERFDANGDGKISISELDSVLKS- 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
            L S+IP EELRSVM+DLDSDKDGYINIDEFAAFCK+PMASDEAGA+ELRDAFDLYDQD 
Sbjct: 61  -LGSSIPYEELRSVMEDLDSDKDGYINIDEFAAFCKEPMASDEAGASELRDAFDLYDQDH 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180
           NGLIS SELHLVLNRLGISCSKEDCQ+MI+SVDSDGDGNVNFEEFRKMMT NS SKAA Q
Sbjct: 121 NGLISHSELHLVLNRLGISCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMTANSNSKAANQ 180

Query: 181 NGTAAAAP 189
           NGTAAAAP
Sbjct: 181 NGTAAAAP 186

[Close]

BLAST of CSPI01G04290 vs. NCBI nr
Match: XP_023529417.1 (probable calcium-binding protein CML27 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 283.5 bits (724), Expect = 1.3e-72
Identity = 143/188 (76.06%), Postives = 166/188 (88.30%), Query Frame = 0


Query: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60
           MATD N I  S S+ +Q+ K  PS LLQD+ ELRKVF+RFDANGDGKIS+SELD+VL S 
Sbjct: 1   MATDSNQIAASNSDTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKS- 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
            L S++P +ELR+VM+DLD+DKDG+INIDEFA FCK+PMAS+EAGA+ELRDAFDLYDQD 
Sbjct: 61  -LGSSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDH 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180
           NGLISQSELHLVLNRLGI+CSKEDCQ+MI+SVDSDGDGNVNFEEFRKMM++NS S+A  Q
Sbjct: 121 NGLISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEAPNQ 180

Query: 181 NGTAAAAP 189
           NGTAA AP
Sbjct: 181 NGTAATAP 186

[Close]

BLAST of CSPI01G04290 vs. NCBI nr
Match: XP_022933917.1 (probable calcium-binding protein CML27 [Cucurbita moschata] >KAG7021647.1 putative calcium-binding protein CML27, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 282.3 bits (721), Expect = 3.0e-72
Identity = 142/188 (75.53%), Postives = 166/188 (88.30%), Query Frame = 0


Query: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60
           MATD N I  S ++ +Q+ K  PS LLQD+ ELRKVF+RFDANGDGKIS+SELD+VL S 
Sbjct: 1   MATDSNQITASNADTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKS- 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
            L S++P +ELR+VM+DLD+DKDG+INIDEFA FCK+PMAS+EAGA+ELRDAFDLYDQD 
Sbjct: 61  -LGSSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDH 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180
           NGLISQSELHLVLNRLGI+CSKEDCQ+MI+SVDSDGDGNVNFEEFRKMM++NS S+A  Q
Sbjct: 121 NGLISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEAPNQ 180

Query: 181 NGTAAAAP 189
           NGTAA AP
Sbjct: 181 NGTAATAP 186

[Close]

BLAST of CSPI01G04290 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 176.4 bits (446), Expect = 2.2e-44
Identity = 93/170 (54.71%), Postives = 125/170 (73.53%), Query Frame = 0


Query: 17  QIAKQNPSVL-LQDDEELRKVFERFDANGDGKISISELDAVLTSLTLKSAIPLEELRSVM 76
           + AK  P+ + + + EEL+KVF++FD+NGDGKIS+ EL  V  +  + ++    EL  V+
Sbjct: 7   ETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKA--MGTSYTETELNRVL 66

Query: 77  DDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNR 136
           +++D+D+DGYIN+DEF+  C+       + AAE+RDAFDLYDQD+NGLIS SELH VLNR
Sbjct: 67  EEVDTDRDGYINLDEFSTLCR-----SSSSAAEIRDAFDLYDQDKNGLISASELHQVLNR 126

Query: 137 LGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQNGTAA 186
           LG+SCS EDC +MI  VD+DGDGNVNFEEF+KMMT +S       NG+AA
Sbjct: 127 LGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS---LLNSNGSAA 166

[Close]

BLAST of CSPI01G04290 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 176.4 bits (446), Expect = 2.2e-44
Identity = 93/170 (54.71%), Postives = 125/170 (73.53%), Query Frame = 0


Query: 17  QIAKQNPSVL-LQDDEELRKVFERFDANGDGKISISELDAVLTSLTLKSAIPLEELRSVM 76
           + AK  P+ + + + EEL+KVF++FD+NGDGKIS+ EL  V  +  + ++    EL  V+
Sbjct: 7   ETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKA--MGTSYTETELNRVL 66

Query: 77  DDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNR 136
           +++D+D+DGYIN+DEF+  C+       + AAE+RDAFDLYDQD+NGLIS SELH VLNR
Sbjct: 67  EEVDTDRDGYINLDEFSTLCR-----SSSSAAEIRDAFDLYDQDKNGLISASELHQVLNR 126

Query: 137 LGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQNGTAA 186
           LG+SCS EDC +MI  VD+DGDGNVNFEEF+KMMT +S       NG+AA
Sbjct: 127 LGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS---LLNSNGSAA 166

[Close]

BLAST of CSPI01G04290 vs. TAIR 10
Match: AT1G73630.1 (EF hand calcium-binding protein family )

HSP 1 Score: 165.2 bits (417), Expect = 5.0e-41
Identity = 87/160 (54.37%), Postives = 112/160 (70.00%), Query Frame = 0


Query: 22  NPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSLTLKSAIPLEELRSVMDDLDSD 81
           N S     D EL+KVF++FDANGDGKIS+SEL  V  S  + ++   EEL  V+D++D D
Sbjct: 10  NKSTTPSTDMELKKVFDKFDANGDGKISVSELGNVFKS--MGTSYTEEELNRVLDEIDID 69

Query: 82  KDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGISCS 141
            DG+IN +EFA  C+       + A E+R+AFDLYDQ++NGLIS SE+H VLNRLG++CS
Sbjct: 70  CDGFINQEEFATICR-----SSSSAVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCS 129

Query: 142 KEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQN 182
            EDC +MI  VD+DGDGNVNFEEF+KMM+     K    N
Sbjct: 130 VEDCVRMIGHVDTDGDGNVNFEEFQKMMSSPELVKGTVAN 162

[Close]

BLAST of CSPI01G04290 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 142.1 bits (357), Expect = 4.5e-34
Identity = 74/146 (50.68%), Postives = 104/146 (71.23%), Query Frame = 0


Query: 31  EELRKVFERFDANGDGKISISELDAVLTSLTLKSAIPLEELRSVMDDLDSDKDGYINIDE 90
           E+++KVF+RFD N DGKISI EL  V+ +L+  ++   EE +++M + D D +G+I++DE
Sbjct: 14  EDIKKVFQRFDKNNDGKISIDELKDVIGALSPNAS--QEETKAMMKEFDLDGNGFIDLDE 73

Query: 91  FAA-FCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGISCSKEDCQKMI 150
           F A F     +S+ +   +L++AFDLYD DRNG IS +ELH V+  LG  CS +DCQ+MI
Sbjct: 74  FVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMI 133

Query: 151 NSVDSDGDGNVNFEEFRKMMTDNSKS 176
           N VDSDGDG V+FEEF+KMM  N  +
Sbjct: 134 NKVDSDGDGCVDFEEFKKMMMINGSA 157

[Close]

BLAST of CSPI01G04290 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 130.6 bits (327), Expect = 1.4e-30
Identity = 71/143 (49.65%), Postives = 90/143 (62.94%), Query Frame = 0


Query: 32  ELRKVFERFDANGDGKISISELDAVLTSLTLKSAIPLEELRSVMDDLDSDKDGYINIDEF 91
           EL  VF++FD NGDGKIS  EL A++TS  L   +P EEL   + ++D   DGYIN +EF
Sbjct: 37  ELEAVFKKFDVNGDGKISSKELGAIMTS--LGHEVPEEELEKAITEIDRKGDGYINFEEF 96

Query: 92  AAFCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGISCSKEDCQKMINS 151
                K M  ++     L+DAF +YD D NG IS  ELH VL  LG  CS  +C+KMI  
Sbjct: 97  VELNTKGMDQNDV-LENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGG 156

Query: 152 VDSDGDGNVNFEEFRKMMTDNSK 175
           VD DGDG ++FEEF+ MMT  S+
Sbjct: 157 VDKDGDGTIDFEEFKIMMTMGSR 176

The following BLAST results are available for this feature:

BLAST of CSPI01G04290 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LE22	3.0e-43	54.71	Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 ...	[more]
Q9C9U8	7.0e-40	54.38	Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 ...	[more]
Q9M7R0	1.9e-37	56.49	Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1	[more]
Q9C8Y1	6.3e-33	50.68	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]
Q0DJV6	3.1e-32	51.01	Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of CSPI01G04290 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LPP8	2.9e-97	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G025020 PE=4 SV=1	[more]
A0A5D3DXJ8	7.4e-93	95.21	Putative calcium-binding protein CML27 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3BX51	7.4e-93	95.21	probable calcium-binding protein CML27 OS=Cucumis melo OX=3656 GN=LOC103494178 P...	[more]
A0A6J1F164	1.4e-72	75.53	probable calcium-binding protein CML27 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1E0A3	1.2e-71	77.13	probable calcium-binding protein CML27 OS=Cucurbita moschata OX=3662 GN=LOC11142...	[more]

BLAST of CSPI01G04290 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004137340.1	6.0e-97	100.00	probable calcium-binding protein CML27 [Cucumis sativus] >KGN63870.1 hypothetica...	[more]
XP_008453475.1	1.5e-92	95.21	PREDICTED: probable calcium-binding protein CML27 [Cucumis melo] >KAA0058132.1 p...	[more]
XP_038878467.1	6.0e-81	85.64	probable calcium-binding protein CML27 [Benincasa hispida]	[more]
XP_023529417.1	1.3e-72	76.06	probable calcium-binding protein CML27 [Cucurbita pepo subsp. pepo]	[more]
XP_022933917.1	3.0e-72	75.53	probable calcium-binding protein CML27 [Cucurbita moschata] >KAG7021647.1 putati...	[more]

BLAST of CSPI01G04290 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G18210.1	2.2e-44	54.71	Calcium-binding EF-hand family protein	[more]
AT1G18210.2	2.2e-44	54.71	Calcium-binding EF-hand family protein	[more]
AT1G73630.1	5.0e-41	54.38	EF hand calcium-binding protein family	[more]
AT1G66400.1	4.5e-34	50.68	calmodulin like 23	[more]
AT1G24620.1	1.4e-30	49.65	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 32..60 e-value: 5.8E-6 score: 35.8 coord: 108..136 e-value: 2.1E-4 score: 30.7 coord: 144..172 e-value: 4.5E-5 score: 32.9 coord: 70..98 e-value: 1.4E-4 score: 31.2
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 108..169 e-value: 5.5E-14 score: 52.4 coord: 31..95 e-value: 4.8E-13 score: 49.3
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 28..63 score: 15.461849
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 104..139 score: 13.09074
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 66..101 score: 12.393354
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 140..175 score: 14.06708
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 108..170 e-value: 9.08346E-21 score: 79.5141
None	No IPR available	GENE3D	1.10.238.10		coord: 8..102 e-value: 4.3E-21 score: 77.4
None	No IPR available	GENE3D	1.10.238.10		coord: 103..183 e-value: 9.7E-24 score: 85.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..24
None	No IPR available	PANTHER	PTHR10891:SF814	CALCIUM-BINDING PROTEIN CML26-RELATED	coord: 17..173
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 17..173
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 153..165
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 41..53
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 79..91
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 117..129
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 31..175

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G04290.1	CSPI01G04290.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding