CSPI01G03060 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G03060
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionCysteine dioxygenase
LocationChr1: 1931437 .. 1934533 (-)
RNA-Seq ExpressionCSPI01G03060
SyntenyCSPI01G03060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAATTACATCATTGCATCGGCTGCGATGCGCGTCGGTATTTCATAATCTCATTCCAAAATCAAAATTTGCATTTAATTTTTTTTTCTTTTTTCTGTTGATTTCAAAATGTTCTCAAAAACCCAAAAAGCGACGGATAAGATGAGATTATTTTTGCAAAACAAGGTGGATTGCTCAGTGTTTCTTCGGTTCTTGAGCATGAGCCACAAGAAGAGCTCCAAAATTCAGGCTATCTACGACCTTTGTAAAAAGAGTTTAACTCCCGCGAACTCTCCACTTTCTTCTCAAACTATCAAGAATCTATGTTCCCTTTTGGGTAATTTCTCTTTCTTGCTTTGAAGTTTATACTGCATTATCTTCTACATTTGGTCTTCCAACTACCCTTTTGTTTCTTCTTGTGATTCTTGTTAATTTCCCATCTGGGTTCATAATTCAAGTGATTCATTCATGGGTTTGCTTAGTTTTGGAGGTTTAATTGATGGATTGGATCCTTGGTTAGTTCGTGAATTGCCATTTGGGAATTTCTTAAACTAAATTGGAAGCTGAATATAGAACCTTTGTTCTTGAGTGTTCTCTAGACATCTTAAGGGATAGACTTCTCTTTTTTAGCCATGGATTTAGCCTAATACTTTGTCCGCTGCATCCTTCAATTGTTTGGAGTGGAGTATGGTAACGTTTTGATGTTACCTTTACTTACTATATTGGAAATAATGATGATTGGAGATCTCAATCAATGGGCGTGTGTATATGTTTGTATTCTCTTATTCTCCCTGATTCCGAGTTTGAGGAATGATTTATTACAAGACAATTGATTATTGTGTTCTTTTGCTGTTCTAAAGAATACTGTTTCAGAAACTAAAGTTTTCTTCAAGTAGGACATACTACATTATGTACTGCGTTAGACTCCACTCTTTAACACATCCCATTTATATGCTCTATTCACAGATACGGTTGGCGCAGGGGATGTTGGGCTTAAAGAAGAGAATGCTGATGACGACCGAGGGCATGGACTTTTTGGACTGAGCCAATTGAATAGGATAGCTCGATGGGCTCAGCCAATAACATACGTTGACATATTTGAATGTGAAAATTTTACGGTCAGTCCCATGCATCCCTTTTCTCAAACAGGTCTTGTTCATTCATTATTTAGCAACCAGAAAGTTGGATTCACAGGTTTTTCAATTTTAATTTCTAGGGAAATGTTTTTGGCAATAGGATTTTGGTTCTACGATATTCATTGTTGAGTTTTGTTCAGGGAGAAAAACCTGAGGCATAGGCCTCTTGCATCTGAGAATTGATTACCTTTTGAATTTTCCACACTGTGACCTAGTTTTTATTTGGAGAAGTTAGTGTCAAAATTACCAGAAACTGTATCTATGGTTGACTTAAACTACCAACGGGCTGTTAGTTATTTTTGAAGCCCCTATTGTAATAAGTATGCTTATGGTATGTACTTAATATCAGCAGATATGTATATTTTGCTTTCCAACTTCTTCAGTCATCCCACTTCACGACCATCCTGGGATGACTGTGTTCAGCAAAGTTCTCTATGGTTCTCTTCATGTCAAAGCATATGACTGGGTGGAACCTCCCTTCATTCACGAAAGCAAGGGATCCACGTATTTTTCAGGTTTGATTTCTAATACCAACTTGTTTTCAGGATATACAAAAACAATCACTTGTTTTGAGTTTTGGCTTGATGGTTGTCTACACTTCTTAAACTCGTAGTTTCACTACATTGCATGCACTGGTCATGTTTTTTGCCTTCACCCTAAAATTAACATTTTTTATATCCATAACAATTGCCTTCACCAGATTGCATGCACTGGTCATGTCATTCCTTGAGCATCTATAGTTTTCAACCATGACCATGAGTGGATGTGTATTGCATATCAGAAAAAAACGATTGTAGATTATTGTGCAGTTTACTGAATCTATGATTAGATAAACATTTGAAATATTACCTTCTGAATGTAACCATCTATTTTCCATGCTTTGTTTTTGGGCTTCAACAATTGTTCAGTATGTTATAGATGAACGAACAGTGTATGTTGGCTTGCATCTCCCTTGCTTGGTGATACGATATTAAACAAAAATGGTATATTAGTTCTTACCTCATAAAATACTTTTGTCTAATCATATATATAAATGACTCACGTTGTTTGCGACCTAATATTTTATATTCTACTAGAACTTGACATCAAGAGTTTCATGTTCTACTGGAACTTGACATCAAGAGTTTCATGTACATTGTGCACCAAGAGAATTGATTACTTCCCTCAACCAAAAAAGAAAACTAAAAGAAATTTTTTTTCCAGCAAGGTTGGCTAAGTTAGCCGTTGATAAAGTTCTAACGGCATCAAGTGCAACGTCAGTTTTGTACCCGAAGGCTGGAGGTAATCTACACTGTTTCACTGCGATTTCGCCTTGTGCTGTGCTTGATATTCTTTCGCCTCCTTATAACGAGGATAAAGGAAGACGATGTACGTACTACCACGACTACCCTTATTCAACTATTTGTAAGTAACATTTTCTCCTTTATGCTTCTTATTTAGTCAACAAATCTCACACAGTGGCTTGCAAAAATCAGCTGCAGATACCAAACTCTATCTAAATGATGAAGAAGTGGAAGATCATGCCTGGCTTGCAAAAATTGAGACCCCCGGTGATCTATATATGCGACCTGGGATGTATGTTGGTCCTGCTATTCAAACTTAGGTGTCAAATAATAATCCGGGGTTATTGAAGTTCTTCCAACATCTACCAGGAAAGCTACCTCTACCCATAGTTTTACTTACAAGATTAGCTCTTGAGAGGGAACTTGGTTTCGTATAGTGCTCTTTATGCTGATTTTACCCCATATACTCTTTGGCACAGTATTTATATAACGCAAAAGCTTAACTTAGTTGCATGTTTTACCTTGGAAACTGGTAGATATAGATGCAAGCAGTTGTTCATTATGTGTGAAAATGTTGTTACGTGCTTTTACATTTTCTTTTGGAAGTAGATGCCTACTCTTGCTTGGTTACTATTATTTGTTTATCAATGGTTGATGACATACTCTAGATGAACTAATCAAACTTCTGATATTTGGCGCTA

mRNA sequence

TAATTACATCATTGCATCGGCTGCGATGCGCGTCGGTATTTCATAATCTCATTCCAAAATCAAAATTTGCATTTAATTTTTTTTTCTTTTTTCTGTTGATTTCAAAATGTTCTCAAAAACCCAAAAAGCGACGGATAAGATGAGATTATTTTTGCAAAACAAGGTGGATTGCTCAGTGTTTCTTCGGTTCTTGAGCATGAGCCACAAGAAGAGCTCCAAAATTCAGGCTATCTACGACCTTTGTAAAAAGAGTTTAACTCCCGCGAACTCTCCACTTTCTTCTCAAACTATCAAGAATCTATGTTCCCTTTTGGATACGGTTGGCGCAGGGGATGTTGGGCTTAAAGAAGAGAATGCTGATGACGACCGAGGGCATGGACTTTTTGGACTGAGCCAATTGAATAGGATAGCTCGATGGGCTCAGCCAATAACATACGTTGACATATTTGAATGTGAAAATTTTACGATATGTATATTTTGCTTTCCAACTTCTTCAGTCATCCCACTTCACGACCATCCTGGGATGACTGTGTTCAGCAAAGTTCTCTATGGTTCTCTTCATGTCAAAGCATATGACTGGGTGGAACCTCCCTTCATTCACGAAAGCAAGGGATCCACGTATTTTTCAGCAAGGTTGGCTAAGTTAGCCGTTGATAAAGTTCTAACGGCATCAAGTGCAACGTCAGTTTTGTACCCGAAGGCTGGAGGTAATCTACACTGTTTCACTGCGATTTCGCCTTGTGCTGTGCTTGATATTCTTTCGCCTCCTTATAACGAGGATAAAGGAAGACGATGTACGTACTACCACGACTACCCTTATTCAACTATTTCTGCAGATACCAAACTCTATCTAAATGATGAAGAAGTGGAAGATCATGCCTGGCTTGCAAAAATTGAGACCCCCGGTGATCTATATATGCGACCTGGGATGTATGTTGGTCCTGCTATTCAAACTTAGGTGTCAAATAATAATCCGGGGTTATTGAAGTTCTTCCAACATCTACCAGGAAAGCTACCTCTACCCATAGTTTTACTTACAAGATTAGCTCTTGAGAGGGAACTTGGTTTCGTATAGTGCTCTTTATGCTGATTTTACCCCATATACTCTTTGGCACAGTATTTATATAACGCAAAAGCTTAACTTAGTTGCATGTTTTACCTTGGAAACTGGTAGATATAGATGCAAGCAGTTGTTCATTATGTGTGAAAATGTTGTTACGTGCTTTTACATTTTCTTTTGGAAGTAGATGCCTACTCTTGCTTGGTTACTATTATTTGTTTATCAATGGTTGATGACATACTCTAGATGAACTAATCAAACTTCTGATATTTGGCGCTA

Coding sequence (CDS)

ATGTTCTCAAAAACCCAAAAAGCGACGGATAAGATGAGATTATTTTTGCAAAACAAGGTGGATTGCTCAGTGTTTCTTCGGTTCTTGAGCATGAGCCACAAGAAGAGCTCCAAAATTCAGGCTATCTACGACCTTTGTAAAAAGAGTTTAACTCCCGCGAACTCTCCACTTTCTTCTCAAACTATCAAGAATCTATGTTCCCTTTTGGATACGGTTGGCGCAGGGGATGTTGGGCTTAAAGAAGAGAATGCTGATGACGACCGAGGGCATGGACTTTTTGGACTGAGCCAATTGAATAGGATAGCTCGATGGGCTCAGCCAATAACATACGTTGACATATTTGAATGTGAAAATTTTACGATATGTATATTTTGCTTTCCAACTTCTTCAGTCATCCCACTTCACGACCATCCTGGGATGACTGTGTTCAGCAAAGTTCTCTATGGTTCTCTTCATGTCAAAGCATATGACTGGGTGGAACCTCCCTTCATTCACGAAAGCAAGGGATCCACGTATTTTTCAGCAAGGTTGGCTAAGTTAGCCGTTGATAAAGTTCTAACGGCATCAAGTGCAACGTCAGTTTTGTACCCGAAGGCTGGAGGTAATCTACACTGTTTCACTGCGATTTCGCCTTGTGCTGTGCTTGATATTCTTTCGCCTCCTTATAACGAGGATAAAGGAAGACGATGTACGTACTACCACGACTACCCTTATTCAACTATTTCTGCAGATACCAAACTCTATCTAAATGATGAAGAAGTGGAAGATCATGCCTGGCTTGCAAAAATTGAGACCCCCGGTGATCTATATATGCGACCTGGGATGTATGTTGGTCCTGCTATTCAAACTTAG

Protein sequence

MFSKTQKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQTIKNLCSLLDTVGAGDVGLKEENADDDRGHGLFGLSQLNRIARWAQPITYVDIFECENFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIHESKGSTYFSARLAKLAVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVLDILSPPYNEDKGRRCTYYHDYPYSTISADTKLYLNDEEVEDHAWLAKIETPGDLYMRPGMYVGPAIQT*
Homology
BLAST of CSPI01G03060 vs. ExPASy Swiss-Prot
Match: Q1G3U6 (Plant cysteine oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=PCO3 PE=1 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 1.4e-89
Identity = 163/285 (57.19%), Postives = 204/285 (71.58%), Query Frame = 0

Query: 1   MFSKTQKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ 60
           M S+  KA +K+   L +K D    +   S + +KS K+Q +YDLCK++ T      +S 
Sbjct: 1   MLSRLFKAGEKVLSNLVSKKD----IYMASRNQEKSPKVQELYDLCKETFTGKAPSPASM 60

Query: 61  TIKNLCSLLDTVGAGDVGLKEENADDDRGHGLFGLSQLNRIARWAQPITYVDIFECENFT 120
            I+ LCS+LD+V   DVGL+E + DDDRG+G+ G+S+ NR+ RWAQPIT++DI EC+ FT
Sbjct: 61  AIQKLCSVLDSVSPADVGLEEVSQDDDRGYGVSGVSRFNRVGRWAQPITFLDIHECDTFT 120

Query: 121 ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPP-FIHESKG-STYFSARLA 180
           +CIFCFPTSSVIPLHDHP M VFSK+LYGSLHVKAYDWVEPP  I + KG      ARLA
Sbjct: 121 MCIFCFPTSSVIPLHDHPEMAVFSKILYGSLHVKAYDWVEPPCIITQDKGVPGSLPARLA 180

Query: 181 KLAVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVLDILSPPYNEDKGRRCTYYHDYPY 240
           KL  DKV+T  S    LYPK GGNLHCFTA++PCAVLDILSPPY E  GR C+YY DYP+
Sbjct: 181 KLVSDKVITPQSEIPALYPKTGGNLHCFTALTPCAVLDILSPPYKESVGRSCSYYMDYPF 240

Query: 241 STISADTKLYLNDEEVED-HAWLAKIETPGDLYMRPGMYVGPAIQ 283
           ST + +  +   DE  ED +AWL +I+TP DL+MRPG Y GP I+
Sbjct: 241 STFALENGMKKVDEGKEDEYAWLVQIDTPDDLHMRPGSYTGPTIR 281

BLAST of CSPI01G03060 vs. ExPASy Swiss-Prot
Match: Q8LGJ5 (Plant cysteine oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=PCO2 PE=1 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 1.3e-50
Identity = 107/248 (43.15%), Postives = 147/248 (59.27%), Query Frame = 0

Query: 39  IQAIYDLCKKSLTPANSPL--SSQTIKNLCSLLDTVGAGDVGLKEENADDDRGHGLFGLS 98
           +Q ++D CKK      S    S + I+ L ++LD +   DVG+  +            +S
Sbjct: 47  VQKLFDTCKKVFADGKSGTVPSQENIEMLRAVLDEIKPEDVGVNPK------------MS 106

Query: 99  QLNRIARWAQP-ITYVDIFECENFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKA 158
                     P +TY+ I+ C  F+ICIFC P S VIPLH+HP MTVFSK+L+G++H+K+
Sbjct: 107 YFRSTVTGRSPLVTYLHIYACHRFSICIFCLPPSGVIPLHNHPEMTVFSKLLFGTMHIKS 166

Query: 159 YDWVEPPFIHESKGSTYFSARLAKLAVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVL 218
           YDWV  P   +    T    RLAK+ VD   TA   TS+LYP  GGN+HCFTA + CAVL
Sbjct: 167 YDWV--PDSPQPSSDT----RLAKVKVDSDFTAPCDTSILYPADGGNMHCFTAKTACAVL 226

Query: 219 DILSPPYNEDKGRRCTYYHDYPYSTISADTKLYLNDEEVEDHAWL-AKIETPGDLYMRPG 278
           D++ PPY++  GR CTYY DYP+S+ S D  + + +EE E +AWL  + E P DL +   
Sbjct: 227 DVIGPPYSDPAGRHCTYYFDYPFSSFSVD-GVVVAEEEKEGYAWLKEREEKPEDLTVTAL 275

Query: 279 MYVGPAIQ 283
           MY GP I+
Sbjct: 287 MYSGPTIK 275

BLAST of CSPI01G03060 vs. ExPASy Swiss-Prot
Match: Q9SJI9 (Plant cysteine oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=PCO4 PE=1 SV=2)

HSP 1 Score: 192.6 bits (488), Expect = 6.1e-48
Identity = 99/244 (40.57%), Postives = 149/244 (61.07%), Query Frame = 0

Query: 40  QAIYDLCKKSLTPANSPLSSQTIKNLCSLLDTVGAGDVGLKEENADDDRGHGLFGLSQLN 99
           Q +Y+ CK S + ++ P++   ++ + ++L+ +   DVG+ E++A   R      L++ N
Sbjct: 6   QRLYNTCKASFS-SDGPITEDALEKVRNVLEKIKPSDVGI-EQDAQLARSRS-GPLNERN 65

Query: 100 RIARWAQPITYVDIFECENFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWV 159
              +    I Y+ + EC++F+I IFC P SS+IPLH+HPGMTV SK++YGS+HVK+YDW+
Sbjct: 66  GSNQSPPAIKYLHLHECDSFSIGIFCMPPSSMIPLHNHPGMTVLSKLVYGSMHVKSYDWL 125

Query: 160 EPPFIHESKGSTYFSARLAKLAVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVLDILS 219
           EP        S    AR AKL  D  +TA S  + LYPK+GGN+HCF AI+ CA+LDIL+
Sbjct: 126 EPQLTEPEDPS---QARPAKLVKDTEMTAQSPVTTLYPKSGGNIHCFKAITHCAILDILA 185

Query: 220 PPYNEDKGRRCTYYHDYPYSTISADTKLYLNDEEVEDHAWLAKIETPGDLYMRPGMYVGP 279
           PPY+ +  R CTY+       +  +  L ++ E V D  WL + + P D  +R   Y GP
Sbjct: 186 PPYSSEHDRHCTYFRKSRREDLPGE--LEVDGEVVTDVTWLEEFQPPDDFVIRRIPYRGP 241

Query: 280 AIQT 284
            I+T
Sbjct: 246 VIRT 241

BLAST of CSPI01G03060 vs. ExPASy Swiss-Prot
Match: Q9LXT4 (Plant cysteine oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=PCO5 PE=1 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 2.6e-46
Identity = 96/246 (39.02%), Postives = 146/246 (59.35%), Query Frame = 0

Query: 39  IQAIYDLCKKSLTPANSPLSSQTIKNLCSLLDTVGAGDVGLKEEN--ADDDRGHGLFGLS 98
           IQ +++ CK SL+P N P+S + +  + ++L+ +   DVGL++E     +  G G    +
Sbjct: 5   IQRLFNTCKSSLSP-NGPVSEEALDKVRNVLEKIKPSDVGLEQEAQLVRNWPGPG----N 64

Query: 99  QLNRIARWAQPITYVDIFECENFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAY 158
           + N        I Y+ + EC++F+I IFC P  S+IPLH+HPGMTV SK++YGS+HVK+Y
Sbjct: 65  ERNGNHHSLPAIKYLQLHECDSFSIGIFCMPPGSIIPLHNHPGMTVLSKLVYGSMHVKSY 124

Query: 159 DWVEPPFIHESKGSTYFSARLAKLAVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVLD 218
           DW EP    +S+      AR AKL  D  +T+ S  + LYP  GGN+HCF AI+ CA+ D
Sbjct: 125 DWAEP---DQSELDDPLQARPAKLVKDIDMTSPSPATTLYPTTGGNIHCFKAITHCAIFD 184

Query: 219 ILSPPYNEDKGRRCTYYHDYPYSTISADTKLYLNDEEVEDHAWLAKIETPGDLYMRPGMY 278
           ILSPPY+   GR C Y+   P   +  + ++ +N E + +  WL + + P +  +    Y
Sbjct: 185 ILSPPYSSTHGRHCNYFRKSPMLDLPGEIEV-MNGEVISNVTWLEEYQPPDNFVIWRVPY 241

Query: 279 VGPAIQ 283
            GP I+
Sbjct: 245 RGPVIR 241

BLAST of CSPI01G03060 vs. ExPASy Swiss-Prot
Match: Q9LXG9 (Plant cysteine oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=PCO1 PE=1 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 8.3e-45
Identity = 102/250 (40.80%), Postives = 148/250 (59.20%), Query Frame = 0

Query: 39  IQAIYDLCKKSLT---PANSPLSSQTIKNLCSLLDTVGAGDVGLKEENADDDRGHGLFGL 98
           ++ +++ CK+  +   P   P S   I+ L  +LD +   DVGL           G+   
Sbjct: 58  VRRLFNTCKEVFSNGGPGVIP-SEDKIQQLREILDDMKPEDVGLTPTMPYFRPNSGV--- 117

Query: 99  SQLNRIARWAQPITYVDIFECENFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKA 158
                 AR + PITY+ + +C+ F+I IFC P S VIPLH+HPGMTVFSK+L+G++H+K+
Sbjct: 118 -----EARSSPPITYLHLHQCDQFSIGIFCLPPSGVIPLHNHPGMTVFSKLLFGTMHIKS 177

Query: 159 YDWVEPPFIHESKGSTYFSARLAKLAVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVL 218
           YDWV    + +SK       RLAKL VD   TA    S+LYP+ GGN+H FTAI+ CAVL
Sbjct: 178 YDWVVDAPMRDSK------TRLAKLKVDSTFTAPCNASILYPEDGGNMHRFTAITACAVL 237

Query: 219 DILSPPYNEDKGRRCTYYHDYPYSTISA-DTKLYLNDEEVEDHAWL-AKIETPGDLYMRP 278
           D+L PPY   +GR CTY+ ++P   +S+ D  +  ++EE E +AWL  + + P D     
Sbjct: 238 DVLGPPYCNPEGRHCTYFLEFPLDKLSSEDDDVLSSEEEKEGYAWLQERDDNPEDHTNVV 292

Query: 279 G-MYVGPAIQ 283
           G +Y GP ++
Sbjct: 298 GALYRGPKVE 292

BLAST of CSPI01G03060 vs. ExPASy TrEMBL
Match: A0A0A0LSH5 (Cysteine dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_1G014440 PE=3 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 4.6e-163
Identity = 281/283 (99.29%), Postives = 281/283 (99.29%), Query Frame = 0

Query: 1   MFSKTQKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ 60
           MFSKTQKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ
Sbjct: 1   MFSKTQKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ 60

Query: 61  TIKNLCSLLDTVGAGDVGLKEENADDDRGHGLFGLSQLNRIARWAQPITYVDIFECENFT 120
           TIKNLCSLLDTVG GDVGLKEENADDDRGHGLFGLSQLNRIARWAQPITYVDIFECENFT
Sbjct: 61  TIKNLCSLLDTVGVGDVGLKEENADDDRGHGLFGLSQLNRIARWAQPITYVDIFECENFT 120

Query: 121 ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIHESKGSTYFSARLAKL 180
           ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIHESKGSTYFSARLAKL
Sbjct: 121 ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIHESKGSTYFSARLAKL 180

Query: 181 AVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVLDILSPPYNEDKGRRCTYYHDYPYST 240
           AVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVLDILSPPYNEDKGRRCTYYHDYPYST
Sbjct: 181 AVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVLDILSPPYNEDKGRRCTYYHDYPYST 240

Query: 241 ISADTKLYLNDEEVEDHAWLAKIETPGDLYMRPGMYVGPAIQT 284
           ISADTKLYLNDEEVEDHAWLAKIETP DLYMRPGMYVGPAIQT
Sbjct: 241 ISADTKLYLNDEEVEDHAWLAKIETPDDLYMRPGMYVGPAIQT 283

BLAST of CSPI01G03060 vs. ExPASy TrEMBL
Match: A0A1S3BWT0 (Cysteine dioxygenase OS=Cucumis melo OX=3656 GN=LOC103494087 PE=3 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 9.9e-158
Identity = 269/282 (95.39%), Postives = 276/282 (97.87%), Query Frame = 0

Query: 1   MFSKTQKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ 60
           MFSKT+KATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ
Sbjct: 1   MFSKTRKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ 60

Query: 61  TIKNLCSLLDTVGAGDVGLKEENADDDRGHGLFGLSQLNRIARWAQPITYVDIFECENFT 120
           TIKNLC+LLDTVGAGDVGLKEEN DDDRGHGLFGLSQLNRIARWAQPITY+DI ECENFT
Sbjct: 61  TIKNLCTLLDTVGAGDVGLKEENVDDDRGHGLFGLSQLNRIARWAQPITYIDICECENFT 120

Query: 121 ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIHESKGSTYFSARLAKL 180
           ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFI ESKGS YFSARLAKL
Sbjct: 121 ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIQESKGSPYFSARLAKL 180

Query: 181 AVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVLDILSPPYNEDKGRRCTYYHDYPYST 240
           AVDKVLTASSATSVLYPKAGGN+HCFTA+SPCAVLD+LSPPYNEDKGRRCTYYHDYPYST
Sbjct: 181 AVDKVLTASSATSVLYPKAGGNIHCFTAVSPCAVLDVLSPPYNEDKGRRCTYYHDYPYST 240

Query: 241 ISADTKLYLNDEEVEDHAWLAKIETPGDLYMRPGMYVGPAIQ 283
           IS+DTKLYLNDEEVEDHAWLAKIETP DLYMRPGMYVGP IQ
Sbjct: 241 ISSDTKLYLNDEEVEDHAWLAKIETPDDLYMRPGMYVGPTIQ 282

BLAST of CSPI01G03060 vs. ExPASy TrEMBL
Match: A0A1S4E0B0 (Cysteine dioxygenase OS=Cucumis melo OX=3656 GN=LOC103494087 PE=3 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 1.5e-150
Identity = 269/333 (80.78%), Postives = 276/333 (82.88%), Query Frame = 0

Query: 1   MFSKTQKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ 60
           MFSKT+KATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ
Sbjct: 1   MFSKTRKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ 60

Query: 61  TIKNLCSLL--------------------------------------------------- 120
           TIKNLC+LL                                                   
Sbjct: 61  TIKNLCTLLEPLFFRECSLNILRDGLLFFSHGFGLILFPLHQFQSSFNCLEWSMLYAPFT 120

Query: 121 DTVGAGDVGLKEENADDDRGHGLFGLSQLNRIARWAQPITYVDIFECENFTICIFCFPTS 180
           DTVGAGDVGLKEEN DDDRGHGLFGLSQLNRIARWAQPITY+DI ECENFTICIFCFPTS
Sbjct: 121 DTVGAGDVGLKEENVDDDRGHGLFGLSQLNRIARWAQPITYIDICECENFTICIFCFPTS 180

Query: 181 SVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIHESKGSTYFSARLAKLAVDKVLTAS 240
           SVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFI ESKGS YFSARLAKLAVDKVLTAS
Sbjct: 181 SVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIQESKGSPYFSARLAKLAVDKVLTAS 240

Query: 241 SATSVLYPKAGGNLHCFTAISPCAVLDILSPPYNEDKGRRCTYYHDYPYSTISADTKLYL 283
           SATSVLYPKAGGN+HCFTA+SPCAVLD+LSPPYNEDKGRRCTYYHDYPYSTIS+DTKLYL
Sbjct: 241 SATSVLYPKAGGNIHCFTAVSPCAVLDVLSPPYNEDKGRRCTYYHDYPYSTISSDTKLYL 300

BLAST of CSPI01G03060 vs. ExPASy TrEMBL
Match: A0A5D3DYD3 (Cysteine dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00050 PE=3 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 9.3e-140
Identity = 246/282 (87.23%), Postives = 252/282 (89.36%), Query Frame = 0

Query: 1   MFSKTQKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ 60
           MFSKT+KATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDL               
Sbjct: 1   MFSKTRKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLY-------------- 60

Query: 61  TIKNLCSLLDTVGAGDVGLKEENADDDRGHGLFGLSQLNRIARWAQPITYVDIFECENFT 120
                     TVGAGDVGLKEEN DDDRGHGLFGLSQLNRIARWAQPITY+DI ECENFT
Sbjct: 61  ----------TVGAGDVGLKEENVDDDRGHGLFGLSQLNRIARWAQPITYIDICECENFT 120

Query: 121 ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIHESKGSTYFSARLAKL 180
           ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFI ESKGS YFSARLAKL
Sbjct: 121 ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIQESKGSPYFSARLAKL 180

Query: 181 AVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVLDILSPPYNEDKGRRCTYYHDYPYST 240
           AVDKVLTASSATSVLYPKAGGN+HCFTA+SPCAVLD+LSPPYNEDKGRRCTYYHDYPYST
Sbjct: 181 AVDKVLTASSATSVLYPKAGGNIHCFTAVSPCAVLDVLSPPYNEDKGRRCTYYHDYPYST 240

Query: 241 ISADTKLYLNDEEVEDHAWLAKIETPGDLYMRPGMYVGPAIQ 283
           IS+DTKLYLNDEEVEDHAWLAKIETP DLYMRPGMYVGPAIQ
Sbjct: 241 ISSDTKLYLNDEEVEDHAWLAKIETPDDLYMRPGMYVGPAIQ 258

BLAST of CSPI01G03060 vs. ExPASy TrEMBL
Match: A0A5A7UQ93 (Cysteine dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003380 PE=3 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 2.7e-139
Identity = 245/282 (86.88%), Postives = 251/282 (89.01%), Query Frame = 0

Query: 1   MFSKTQKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ 60
           MFSKT+KATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDL               
Sbjct: 1   MFSKTRKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLY-------------- 60

Query: 61  TIKNLCSLLDTVGAGDVGLKEENADDDRGHGLFGLSQLNRIARWAQPITYVDIFECENFT 120
                     TVGAGDVGLKEEN DDDRGHGLFGLSQLNRIARWAQPITY+DI ECENFT
Sbjct: 61  ----------TVGAGDVGLKEENVDDDRGHGLFGLSQLNRIARWAQPITYIDICECENFT 120

Query: 121 ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIHESKGSTYFSARLAKL 180
           ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFI ESKGS YFSARLAKL
Sbjct: 121 ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIQESKGSPYFSARLAKL 180

Query: 181 AVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVLDILSPPYNEDKGRRCTYYHDYPYST 240
           AVDKVLTASSATSVLYPKAGGN+HCFTA+SPCAVLD+LSPPYNEDKGRRCTYYHDYPYST
Sbjct: 181 AVDKVLTASSATSVLYPKAGGNIHCFTAVSPCAVLDVLSPPYNEDKGRRCTYYHDYPYST 240

Query: 241 ISADTKLYLNDEEVEDHAWLAKIETPGDLYMRPGMYVGPAIQ 283
           IS+DTKLYLNDEEVEDHAWLAKIETP DLYMRPGMYVGP IQ
Sbjct: 241 ISSDTKLYLNDEEVEDHAWLAKIETPDDLYMRPGMYVGPTIQ 258

BLAST of CSPI01G03060 vs. NCBI nr
Match: XP_011648514.1 (plant cysteine oxidase 3 isoform X2 [Cucumis sativus])

HSP 1 Score: 583.6 bits (1503), Expect = 9.4e-163
Identity = 281/283 (99.29%), Postives = 281/283 (99.29%), Query Frame = 0

Query: 1   MFSKTQKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ 60
           MFSKTQKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ
Sbjct: 1   MFSKTQKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ 60

Query: 61  TIKNLCSLLDTVGAGDVGLKEENADDDRGHGLFGLSQLNRIARWAQPITYVDIFECENFT 120
           TIKNLCSLLDTVG GDVGLKEENADDDRGHGLFGLSQLNRIARWAQPITYVDIFECENFT
Sbjct: 61  TIKNLCSLLDTVGVGDVGLKEENADDDRGHGLFGLSQLNRIARWAQPITYVDIFECENFT 120

Query: 121 ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIHESKGSTYFSARLAKL 180
           ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIHESKGSTYFSARLAKL
Sbjct: 121 ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIHESKGSTYFSARLAKL 180

Query: 181 AVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVLDILSPPYNEDKGRRCTYYHDYPYST 240
           AVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVLDILSPPYNEDKGRRCTYYHDYPYST
Sbjct: 181 AVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVLDILSPPYNEDKGRRCTYYHDYPYST 240

Query: 241 ISADTKLYLNDEEVEDHAWLAKIETPGDLYMRPGMYVGPAIQT 284
           ISADTKLYLNDEEVEDHAWLAKIETP DLYMRPGMYVGPAIQT
Sbjct: 241 ISADTKLYLNDEEVEDHAWLAKIETPDDLYMRPGMYVGPAIQT 283

BLAST of CSPI01G03060 vs. NCBI nr
Match: XP_004138203.1 (plant cysteine oxidase 3 isoform X1 [Cucumis sativus])

HSP 1 Score: 578.9 bits (1491), Expect = 2.3e-161
Identity = 281/284 (98.94%), Postives = 281/284 (98.94%), Query Frame = 0

Query: 1   MFSKTQKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ 60
           MFSKTQKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ
Sbjct: 1   MFSKTQKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ 60

Query: 61  TIKNLCSLLDTVGAGDVGLKEENADDDRGHGLFGLSQLNRIARWAQPITYVDIFECENFT 120
           TIKNLCSLLDTVG GDVGLKEENADDDRGHGLFGLSQLNRIARWAQPITYVDIFECENFT
Sbjct: 61  TIKNLCSLLDTVGVGDVGLKEENADDDRGHGLFGLSQLNRIARWAQPITYVDIFECENFT 120

Query: 121 -ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIHESKGSTYFSARLAK 180
            ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIHESKGSTYFSARLAK
Sbjct: 121 QICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIHESKGSTYFSARLAK 180

Query: 181 LAVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVLDILSPPYNEDKGRRCTYYHDYPYS 240
           LAVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVLDILSPPYNEDKGRRCTYYHDYPYS
Sbjct: 181 LAVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVLDILSPPYNEDKGRRCTYYHDYPYS 240

Query: 241 TISADTKLYLNDEEVEDHAWLAKIETPGDLYMRPGMYVGPAIQT 284
           TISADTKLYLNDEEVEDHAWLAKIETP DLYMRPGMYVGPAIQT
Sbjct: 241 TISADTKLYLNDEEVEDHAWLAKIETPDDLYMRPGMYVGPAIQT 284

BLAST of CSPI01G03060 vs. NCBI nr
Match: XP_008453337.2 (PREDICTED: plant cysteine oxidase 3 isoform X2 [Cucumis melo])

HSP 1 Score: 565.8 bits (1457), Expect = 2.0e-157
Identity = 269/282 (95.39%), Postives = 276/282 (97.87%), Query Frame = 0

Query: 1   MFSKTQKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ 60
           MFSKT+KATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ
Sbjct: 1   MFSKTRKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ 60

Query: 61  TIKNLCSLLDTVGAGDVGLKEENADDDRGHGLFGLSQLNRIARWAQPITYVDIFECENFT 120
           TIKNLC+LLDTVGAGDVGLKEEN DDDRGHGLFGLSQLNRIARWAQPITY+DI ECENFT
Sbjct: 61  TIKNLCTLLDTVGAGDVGLKEENVDDDRGHGLFGLSQLNRIARWAQPITYIDICECENFT 120

Query: 121 ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIHESKGSTYFSARLAKL 180
           ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFI ESKGS YFSARLAKL
Sbjct: 121 ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIQESKGSPYFSARLAKL 180

Query: 181 AVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVLDILSPPYNEDKGRRCTYYHDYPYST 240
           AVDKVLTASSATSVLYPKAGGN+HCFTA+SPCAVLD+LSPPYNEDKGRRCTYYHDYPYST
Sbjct: 181 AVDKVLTASSATSVLYPKAGGNIHCFTAVSPCAVLDVLSPPYNEDKGRRCTYYHDYPYST 240

Query: 241 ISADTKLYLNDEEVEDHAWLAKIETPGDLYMRPGMYVGPAIQ 283
           IS+DTKLYLNDEEVEDHAWLAKIETP DLYMRPGMYVGP IQ
Sbjct: 241 ISSDTKLYLNDEEVEDHAWLAKIETPDDLYMRPGMYVGPTIQ 282

BLAST of CSPI01G03060 vs. NCBI nr
Match: KAE8652442.1 (hypothetical protein Csa_013638 [Cucumis sativus])

HSP 1 Score: 546.2 bits (1406), Expect = 1.7e-151
Identity = 269/283 (95.05%), Postives = 269/283 (95.05%), Query Frame = 0

Query: 1   MFSKTQKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ 60
           MFSKTQKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ
Sbjct: 1   MFSKTQKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ 60

Query: 61  TIKNLCSLLDTVGAGDVGLKEENADDDRGHGLFGLSQLNRIARWAQPITYVDIFECENFT 120
           TIKNLCSLLDTVG GDVGLKEENADDDRGHGLFGLSQLNRIARWAQPITYVDIFECENFT
Sbjct: 61  TIKNLCSLLDTVGVGDVGLKEENADDDRGHGLFGLSQLNRIARWAQPITYVDIFECENFT 120

Query: 121 ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIHESKGSTYFSARLAKL 180
           ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIHESKGSTYFSARLAKL
Sbjct: 121 ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIHESKGSTYFSARLAKL 180

Query: 181 AVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVLDILSPPYNEDKGRRCTYYHDYPYST 240
           AVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVLDILSPPYNEDKGRR           
Sbjct: 181 AVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVLDILSPPYNEDKGRR----------- 240

Query: 241 ISADTKLYLNDEEVEDHAWLAKIETPGDLYMRPGMYVGPAIQT 284
            SADTKLYLNDEEVEDHAWLAKIETP DLYMRPGMYVGPAIQT
Sbjct: 241 -SADTKLYLNDEEVEDHAWLAKIETPDDLYMRPGMYVGPAIQT 271

BLAST of CSPI01G03060 vs. NCBI nr
Match: XP_038879681.1 (plant cysteine oxidase 3 isoform X1 [Benincasa hispida])

HSP 1 Score: 543.1 bits (1398), Expect = 1.4e-150
Identity = 258/282 (91.49%), Postives = 269/282 (95.39%), Query Frame = 0

Query: 1   MFSKTQKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ 60
           MFSKTQKATDK+RL LQN VDCSVFLRFLSMSHKKSSKIQAIYDLCKK+LTPANSPLSSQ
Sbjct: 1   MFSKTQKATDKIRLLLQNTVDCSVFLRFLSMSHKKSSKIQAIYDLCKKTLTPANSPLSSQ 60

Query: 61  TIKNLCSLLDTVGAGDVGLKEENADDDRGHGLFGLSQLNRIARWAQPITYVDIFECENFT 120
           TI+NLCSL+DTVG GDVGLKEEN DDDRGHGLFGLSQLNRIARWAQPITYVDIFECE+FT
Sbjct: 61  TIQNLCSLMDTVGPGDVGLKEENVDDDRGHGLFGLSQLNRIARWAQPITYVDIFECESFT 120

Query: 121 ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIHESKGSTYFSARLAKL 180
           ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFI ESKGSTYF ARLAKL
Sbjct: 121 ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIQESKGSTYFPARLAKL 180

Query: 181 AVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVLDILSPPYNEDKGRRCTYYHDYPYST 240
           AVDKVLTASSATSVLYPK GGNLHCFTA+SPCAVLD+LSPPYNEDKGRRCTYYHDYPYST
Sbjct: 181 AVDKVLTASSATSVLYPKTGGNLHCFTAVSPCAVLDVLSPPYNEDKGRRCTYYHDYPYST 240

Query: 241 ISADTKLYLNDEEVEDHAWLAKIETPGDLYMRPGMYVGPAIQ 283
           IS ++K  LNDEEVED+AWLAKIETP DLYMRPGMY GP I+
Sbjct: 241 ISIESKPNLNDEEVEDYAWLAKIETPDDLYMRPGMYAGPGIE 282

BLAST of CSPI01G03060 vs. TAIR 10
Match: AT1G18490.1 (Protein of unknown function (DUF1637) )

HSP 1 Score: 330.9 bits (847), Expect = 1.0e-90
Identity = 163/285 (57.19%), Postives = 204/285 (71.58%), Query Frame = 0

Query: 1   MFSKTQKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ 60
           M S+  KA +K+   L +K D    +   S + +KS K+Q +YDLCK++ T      +S 
Sbjct: 1   MLSRLFKAGEKVLSNLVSKKD----IYMASRNQEKSPKVQELYDLCKETFTGKAPSPASM 60

Query: 61  TIKNLCSLLDTVGAGDVGLKEENADDDRGHGLFGLSQLNRIARWAQPITYVDIFECENFT 120
            I+ LCS+LD+V   DVGL+E + DDDRG+G+ G+S+ NR+ RWAQPIT++DI EC+ FT
Sbjct: 61  AIQKLCSVLDSVSPADVGLEEVSQDDDRGYGVSGVSRFNRVGRWAQPITFLDIHECDTFT 120

Query: 121 ICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPP-FIHESKG-STYFSARLA 180
           +CIFCFPTSSVIPLHDHP M VFSK+LYGSLHVKAYDWVEPP  I + KG      ARLA
Sbjct: 121 MCIFCFPTSSVIPLHDHPEMAVFSKILYGSLHVKAYDWVEPPCIITQDKGVPGSLPARLA 180

Query: 181 KLAVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVLDILSPPYNEDKGRRCTYYHDYPY 240
           KL  DKV+T  S    LYPK GGNLHCFTA++PCAVLDILSPPY E  GR C+YY DYP+
Sbjct: 181 KLVSDKVITPQSEIPALYPKTGGNLHCFTALTPCAVLDILSPPYKESVGRSCSYYMDYPF 240

Query: 241 STISADTKLYLNDEEVED-HAWLAKIETPGDLYMRPGMYVGPAIQ 283
           ST + +  +   DE  ED +AWL +I+TP DL+MRPG Y GP I+
Sbjct: 241 STFALENGMKKVDEGKEDEYAWLVQIDTPDDLHMRPGSYTGPTIR 281

BLAST of CSPI01G03060 vs. TAIR 10
Match: AT5G39890.1 (Protein of unknown function (DUF1637) )

HSP 1 Score: 201.4 bits (511), Expect = 9.4e-52
Identity = 107/248 (43.15%), Postives = 147/248 (59.27%), Query Frame = 0

Query: 39  IQAIYDLCKKSLTPANSPL--SSQTIKNLCSLLDTVGAGDVGLKEENADDDRGHGLFGLS 98
           +Q ++D CKK      S    S + I+ L ++LD +   DVG+  +            +S
Sbjct: 47  VQKLFDTCKKVFADGKSGTVPSQENIEMLRAVLDEIKPEDVGVNPK------------MS 106

Query: 99  QLNRIARWAQP-ITYVDIFECENFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKA 158
                     P +TY+ I+ C  F+ICIFC P S VIPLH+HP MTVFSK+L+G++H+K+
Sbjct: 107 YFRSTVTGRSPLVTYLHIYACHRFSICIFCLPPSGVIPLHNHPEMTVFSKLLFGTMHIKS 166

Query: 159 YDWVEPPFIHESKGSTYFSARLAKLAVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVL 218
           YDWV  P   +    T    RLAK+ VD   TA   TS+LYP  GGN+HCFTA + CAVL
Sbjct: 167 YDWV--PDSPQPSSDT----RLAKVKVDSDFTAPCDTSILYPADGGNMHCFTAKTACAVL 226

Query: 219 DILSPPYNEDKGRRCTYYHDYPYSTISADTKLYLNDEEVEDHAWL-AKIETPGDLYMRPG 278
           D++ PPY++  GR CTYY DYP+S+ S D  + + +EE E +AWL  + E P DL +   
Sbjct: 227 DVIGPPYSDPAGRHCTYYFDYPFSSFSVD-GVVVAEEEKEGYAWLKEREEKPEDLTVTAL 275

Query: 279 MYVGPAIQ 283
           MY GP I+
Sbjct: 287 MYSGPTIK 275

BLAST of CSPI01G03060 vs. TAIR 10
Match: AT2G42670.1 (Protein of unknown function (DUF1637) )

HSP 1 Score: 192.6 bits (488), Expect = 4.4e-49
Identity = 99/244 (40.57%), Postives = 149/244 (61.07%), Query Frame = 0

Query: 40  QAIYDLCKKSLTPANSPLSSQTIKNLCSLLDTVGAGDVGLKEENADDDRGHGLFGLSQLN 99
           Q +Y+ CK S + ++ P++   ++ + ++L+ +   DVG+ E++A   R      L++ N
Sbjct: 6   QRLYNTCKASFS-SDGPITEDALEKVRNVLEKIKPSDVGI-EQDAQLARSRS-GPLNERN 65

Query: 100 RIARWAQPITYVDIFECENFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWV 159
              +    I Y+ + EC++F+I IFC P SS+IPLH+HPGMTV SK++YGS+HVK+YDW+
Sbjct: 66  GSNQSPPAIKYLHLHECDSFSIGIFCMPPSSMIPLHNHPGMTVLSKLVYGSMHVKSYDWL 125

Query: 160 EPPFIHESKGSTYFSARLAKLAVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVLDILS 219
           EP        S    AR AKL  D  +TA S  + LYPK+GGN+HCF AI+ CA+LDIL+
Sbjct: 126 EPQLTEPEDPS---QARPAKLVKDTEMTAQSPVTTLYPKSGGNIHCFKAITHCAILDILA 185

Query: 220 PPYNEDKGRRCTYYHDYPYSTISADTKLYLNDEEVEDHAWLAKIETPGDLYMRPGMYVGP 279
           PPY+ +  R CTY+       +  +  L ++ E V D  WL + + P D  +R   Y GP
Sbjct: 186 PPYSSEHDRHCTYFRKSRREDLPGE--LEVDGEVVTDVTWLEEFQPPDDFVIRRIPYRGP 241

Query: 280 AIQT 284
            I+T
Sbjct: 246 VIRT 241

BLAST of CSPI01G03060 vs. TAIR 10
Match: AT2G42670.2 (Protein of unknown function (DUF1637) )

HSP 1 Score: 192.6 bits (488), Expect = 4.4e-49
Identity = 99/244 (40.57%), Postives = 149/244 (61.07%), Query Frame = 0

Query: 40  QAIYDLCKKSLTPANSPLSSQTIKNLCSLLDTVGAGDVGLKEENADDDRGHGLFGLSQLN 99
           Q +Y+ CK S + ++ P++   ++ + ++L+ +   DVG+ E++A   R      L++ N
Sbjct: 6   QRLYNTCKASFS-SDGPITEDALEKVRNVLEKIKPSDVGI-EQDAQLARSRS-GPLNERN 65

Query: 100 RIARWAQPITYVDIFECENFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWV 159
              +    I Y+ + EC++F+I IFC P SS+IPLH+HPGMTV SK++YGS+HVK+YDW+
Sbjct: 66  GSNQSPPAIKYLHLHECDSFSIGIFCMPPSSMIPLHNHPGMTVLSKLVYGSMHVKSYDWL 125

Query: 160 EPPFIHESKGSTYFSARLAKLAVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVLDILS 219
           EP        S    AR AKL  D  +TA S  + LYPK+GGN+HCF AI+ CA+LDIL+
Sbjct: 126 EPQLTEPEDPSQ--EARPAKLVKDTEMTAQSPVTTLYPKSGGNIHCFKAITHCAILDILA 185

Query: 220 PPYNEDKGRRCTYYHDYPYSTISADTKLYLNDEEVEDHAWLAKIETPGDLYMRPGMYVGP 279
           PPY+ +  R CTY+       +  +  L ++ E V D  WL + + P D  +R   Y GP
Sbjct: 186 PPYSSEHDRHCTYFRKSRREDLPGE--LEVDGEVVTDVTWLEEFQPPDDFVIRRIPYRGP 242

Query: 280 AIQT 284
            I+T
Sbjct: 246 VIRT 242

BLAST of CSPI01G03060 vs. TAIR 10
Match: AT3G58670.1 (Protein of unknown function (DUF1637) )

HSP 1 Score: 187.2 bits (474), Expect = 1.8e-47
Identity = 96/246 (39.02%), Postives = 146/246 (59.35%), Query Frame = 0

Query: 39  IQAIYDLCKKSLTPANSPLSSQTIKNLCSLLDTVGAGDVGLKEEN--ADDDRGHGLFGLS 98
           IQ +++ CK SL+P N P+S + +  + ++L+ +   DVGL++E     +  G G    +
Sbjct: 5   IQRLFNTCKSSLSP-NGPVSEEALDKVRNVLEKIKPSDVGLEQEAQLVRNWPGPG----N 64

Query: 99  QLNRIARWAQPITYVDIFECENFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAY 158
           + N        I Y+ + EC++F+I IFC P  S+IPLH+HPGMTV SK++YGS+HVK+Y
Sbjct: 65  ERNGNHHSLPAIKYLQLHECDSFSIGIFCMPPGSIIPLHNHPGMTVLSKLVYGSMHVKSY 124

Query: 159 DWVEPPFIHESKGSTYFSARLAKLAVDKVLTASSATSVLYPKAGGNLHCFTAISPCAVLD 218
           DW EP    +S+      AR AKL  D  +T+ S  + LYP  GGN+HCF AI+ CA+ D
Sbjct: 125 DWAEP---DQSELDDPLQARPAKLVKDIDMTSPSPATTLYPTTGGNIHCFKAITHCAIFD 184

Query: 219 ILSPPYNEDKGRRCTYYHDYPYSTISADTKLYLNDEEVEDHAWLAKIETPGDLYMRPGMY 278
           ILSPPY+   GR C Y+   P   +  + ++ +N E + +  WL + + P +  +    Y
Sbjct: 185 ILSPPYSSTHGRHCNYFRKSPMLDLPGEIEV-MNGEVISNVTWLEEYQPPDNFVIWRVPY 241

Query: 279 VGPAIQ 283
            GP I+
Sbjct: 245 RGPVIR 241

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q1G3U61.4e-8957.19Plant cysteine oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=PCO3 PE=1 SV=1[more]
Q8LGJ51.3e-5043.15Plant cysteine oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=PCO2 PE=1 SV=1[more]
Q9SJI96.1e-4840.57Plant cysteine oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=PCO4 PE=1 SV=2[more]
Q9LXT42.6e-4639.02Plant cysteine oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=PCO5 PE=1 SV=1[more]
Q9LXG98.3e-4540.80Plant cysteine oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=PCO1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LSH54.6e-16399.29Cysteine dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_1G014440 PE=3 SV=1[more]
A0A1S3BWT09.9e-15895.39Cysteine dioxygenase OS=Cucumis melo OX=3656 GN=LOC103494087 PE=3 SV=1[more]
A0A1S4E0B01.5e-15080.78Cysteine dioxygenase OS=Cucumis melo OX=3656 GN=LOC103494087 PE=3 SV=1[more]
A0A5D3DYD39.3e-14087.23Cysteine dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629... [more]
A0A5A7UQ932.7e-13986.88Cysteine dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274... [more]
Match NameE-valueIdentityDescription
XP_011648514.19.4e-16399.29plant cysteine oxidase 3 isoform X2 [Cucumis sativus][more]
XP_004138203.12.3e-16198.94plant cysteine oxidase 3 isoform X1 [Cucumis sativus][more]
XP_008453337.22.0e-15795.39PREDICTED: plant cysteine oxidase 3 isoform X2 [Cucumis melo][more]
KAE8652442.11.7e-15195.05hypothetical protein Csa_013638 [Cucumis sativus][more]
XP_038879681.11.4e-15091.49plant cysteine oxidase 3 isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G18490.11.0e-9057.19Protein of unknown function (DUF1637) [more]
AT5G39890.19.4e-5243.15Protein of unknown function (DUF1637) [more]
AT2G42670.14.4e-4940.57Protein of unknown function (DUF1637) [more]
AT2G42670.24.4e-4940.57Protein of unknown function (DUF1637) [more]
AT3G58670.11.8e-4739.02Protein of unknown function (DUF1637) [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 95..246
e-value: 1.3E-12
score: 49.2
IPR012864Cysteine oxygenase/2-aminoethanethiol dioxygenasePFAMPF07847PCO_ADOcoord: 68..281
e-value: 6.4E-71
score: 238.0
NoneNo IPR availablePANTHERPTHR22966:SF292-AMINOETHANETHIOL DIOXYGENASEcoord: 31..282
NoneNo IPR availablePANTHERPTHR22966UNCHARACTERIZEDcoord: 31..282
NoneNo IPR availableCDDcd20289cupin_ADOcoord: 117..223
e-value: 1.37486E-49
score: 157.712
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 36..237

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G03060.1CSPI01G03060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0017172 cysteine dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen