CSPI01G03020 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G03020
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionPhosphopyruvate hydratase
LocationChr1: 1920959 .. 1924438 (+)
RNA-Seq ExpressionCSPI01G03020
SyntenyCSPI01G03020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCAGGGCCAAAATTCGATTGCCAAGTGAGTCGCTTTCAACATTCTCGTCGCCATTTCCCAACAACCACGCTAACCCCTGCTGGCTGCTGCTCTGCTTTGAACGCCCCTTTCCTTTGGCGGACTTTCCCACTTTCTTCTTCTTCTTCTACATATACATTCACTCACTTCTCCATTTTCATTTCTTCTCATCAGAACTTCAATGGCTTCTCTTCCTTCCCCACCCACCATGAATCTCCTCAACAAATCCCTCTCTCTTTCCCCACCTCAATCCTTCATTGCCTTAATACCTTCTTCTTCTTCTTCTTCCTCCAAACCCCTTCGTTCCCCTACTATTCGCTGTTCTGTTGCTGTGGCTCCTTCTGTTTCAACCAACGTGTCCAAGGAATTCAAGTTGAAGTCCCTCAAGGCCCGTCAGATCATTGACAGTAGAGGCAATCCTACGGTCGAGGTTGATTTGATTACTGACGATCTCTACCGATCCGCTGTTCCTAGTGGGGCTTCTACTGGGATCTATGAGGCGTTGGAGCTTAGAGATGGGGACAAAACTGTTTATGGAGGTAAAGGTGTGCTTACTGCTGTTAAGAATATCAATGATATCTTGGCTCCTAAGCTCGTTGGCGTCGATGTCAGGTTGTTGATTCGATTCTTTCTTTATTTGCTTTTATGAGCATTAATTTGAATGTTTGTTGGATTGTAATACTGACTTCTAATTGTGATCATTGGAATTTGGATTTTAGTTCGTTTGATTATTGACTGTAAATCTGCTTTTGTAATTAAGTAGACCTTTTATAATCTATGTCCACGAGTCTAGACTTTAAGTTGCAGAAAGAGACTTGTTTTCGAAAATTGACGTTGGTTTTGAATGAATTTCTAGGTGCTTAAAAACATTTTTTTTTCGCTTAAAACTACTTCTCCAAATATTAGAAACTTATTCCAAAAACACCCTTCAAGTACTTATCTTGATTGTGAGTCTTGTGTGTAAAAGCCAAAAGGTGTCTTAGTTCATATTGAGTTTTCAACCTAATTGTTGCGAGGCAACGACGTGAATGGAACAGAATAATGGTGGAAATTTTCGTTATTGGGGATTCTCAAACTATATGCTTTTTTTTTTTTTTTTTTTTTTGCTGTACTTACTGGTCGGAAAAAGTTGGTAGCCTTTCTATTCAATTTATTACTGAAGATGTAAAATTCCAAGCCTTTTCATTGGTTTTGAAAGAGTAAAGGTATTCAAAATAACATGCCAACATAAAGTCTTGCCTAAAGAAAGAGTTTGTCGTTGGTCTGTCTTTGTGATAGTTGTTTAAGAATAGAATCAATTTTCATTGATGAATGAAATAAATAGTCAAACTCCCAAACACCTTAGTCTCTGTGTTACAGGATAAGCTTCCAGTTATTGATCAAAGAAAAAAGACTAGAATGAGAAGAAAAGAGGGGGAAAGAAGTGTCTTTGTGGGAGTTGGTTCACTTTTCAGTACTTCTGCTTCCTAACCTAAGTTTTTCAACTTCTGTAGGAATCAAGAAGAAGTTGATGCAATCATGCTGGAAATTGATGGAACTCCAAACAAGTCGGAACTTGGGGCAAATGCTATACTAGGAGTTTCTTTGAGTGTCTGCAGAGCTGGTGCTGGCGCTAAGGGACTACCATTGTATAGACACATCCAAGAATTATCAGGCACCAAGGAACTTGTTATGCCAGTTCCTGCTTTTAATGTTATAAACGGGGGTAGCCATGCTGGAAATAATCTAGCAATGCAAGAATTTATGATCTTACCTGTAGGTGCTTCATCTTTCGCAGAGGCACTTCGGATGGGAAGTGAAGGTTGGCAGCTGCTTCTCAAGTGCTAAAATTTTCTTTTCCATTGTTATGGTGTTAGTTTATGATTCATTTTGCACCTCATCTTCGTCATTTTGTACTTAAACAAGTTATTTATGAGTAGCCTGATTTTGGTATTTTCTAATGAACAGTTTATCACACACTAAAGGGAATTATTAAAGCAAAATATGGACAAGATGCCTGCAATGTTGGAGATGAGGGTGGATTCGCTCCAAATGTTCAAGATAACAGAGAAGGACTGGTGTTGCTTATTGATGCCATTGAAAAGGCTGGCTATACAGGAAAGGTGCAGGATTGTAAAATTTCCTTATTGTTTTGAAATGGTGTAGAAGTTTTCTTACATGTCCTGCTAAGACCAAAAAAAATAAATGAAAATTTACTTTGATACAGATCAAAATAGGAATGGATGTTGCAGCTTCCGAGTTTTTCACTGCAGAGGGAAAATATGATTTGAACTTCAAGAAACAGCCAAATGATGGAGCTCATGTCCACTCAGCTCATGGCCTTGGCGAACTCTATAAACAGTTTGTCAAAGATTTTCCCATTGTTTCCATCGAGGACCCCTTTGATCAAGATGATTGGAGTTCCTGGGCTTCTCTACAATCTTCCGTTGATATCCAACTTGTGGGAGATGATTTGCTGGTAACAAATCCAAAGAGAATTGCCGAAGGCATTCAGAAAAAAGCTTGTAATGCTTTACTTCTGAAGGTAAGCAGCCTCTGATTTTGTTCTTTCCTTTTTATCTCTGTTCTAGACTAATTGTAGGATTTAGGTTGGTCCTTAAAAATTTTACTTGTTTGCTGAATGTTTGGTTTTCCATTGATTTAGGTTGGTCCTTAGAAACATGTGATTTTGATGCTGCAGCTTGCTCATATGTTAGAAACTTCTCCTGCAGGTTAACCAAATTGGATCAGTGACTGAATCAATTCAAGCTGCTCTTGATTCCAAGGCTGCTGGCTGGGGAGTGATGGTCAGCCACCGAAGTGGTGAAACTGAAGATAATTTTATTGCGGATCTTTCAGTTGGCTTGGCTAGTGGACAGGTTTCATTCATCTTCAGCTTTTCTGTCTACAACTATAAGTCCATAACGCATGTATGTTGTTGACATAATTCACAACTGAATATTGTGTTACTATTCTACAGATCAAAACTGGAGCTCCATGCCGAAGCGAACGGTTAGCCAAATATAATCAGGTAAAGTTTGACCAAATCTGGACATCCAAAATTATTGTCCTTGTATTGCTAGAATATTTCTAGTTTTGCTCAATGTATTTATATATTCTTTTTCTTTTTCTTTTTTAATTTTGATGCAGCTTCTTCGCATTGAAGAAGAACTCGGCAGTGTTCGATATGCCGGTGCGTCTTTCAGATCACCTTAGGAGTTAAAAGTTCAAGGTGATGACTGTGTTGATGTATCAACTTTAGGCTTGATAGAAGAACATGTAGAGGATTTCTTCAGAAATAATATGCTCAACTTTTCTGTCCAAGGTGTTAGAATCTGACACGTTACGAAGTTCATTCGAGTGTTGCCATGAGAGTATTTAAATTTTTGTTATGAACTATTCGTTTCAAAATATTGCAGTGTTTTGATTCACAAACTACCTCGTGGGGTTCTCTTTTCTTCAAAGCTATTCCTTACAGCCAA

mRNA sequence

TTCAGGGCCAAAATTCGATTGCCAAGTGAGTCGCTTTCAACATTCTCGTCGCCATTTCCCAACAACCACGCTAACCCCTGCTGGCTGCTGCTCTGCTTTGAACGCCCCTTTCCTTTGGCGGACTTTCCCACTTTCTTCTTCTTCTTCTACATATACATTCACTCACTTCTCCATTTTCATTTCTTCTCATCAGAACTTCAATGGCTTCTCTTCCTTCCCCACCCACCATGAATCTCCTCAACAAATCCCTCTCTCTTTCCCCACCTCAATCCTTCATTGCCTTAATACCTTCTTCTTCTTCTTCTTCCTCCAAACCCCTTCGTTCCCCTACTATTCGCTGTTCTGTTGCTGTGGCTCCTTCTGTTTCAACCAACGTGTCCAAGGAATTCAAGTTGAAGTCCCTCAAGGCCCGTCAGATCATTGACAGTAGAGGCAATCCTACGGTCGAGGTTGATTTGATTACTGACGATCTCTACCGATCCGCTGTTCCTAGTGGGGCTTCTACTGGGATCTATGAGGCGTTGGAGCTTAGAGATGGGGACAAAACTGTTTATGGAGGTAAAGGTGTGCTTACTGCTGTTAAGAATATCAATGATATCTTGGCTCCTAAGCTCGTTGGCGTCGATGTCAGGAATCAAGAAGAAGTTGATGCAATCATGCTGGAAATTGATGGAACTCCAAACAAGTCGGAACTTGGGGCAAATGCTATACTAGGAGTTTCTTTGAGTGTCTGCAGAGCTGGTGCTGGCGCTAAGGGACTACCATTGTATAGACACATCCAAGAATTATCAGGCACCAAGGAACTTGTTATGCCAGTTCCTGCTTTTAATGTTATAAACGGGGGTAGCCATGCTGGAAATAATCTAGCAATGCAAGAATTTATGATCTTACCTGTAGGTGCTTCATCTTTCGCAGAGGCACTTCGGATGGGAAGTGAAGTTTATCACACACTAAAGGGAATTATTAAAGCAAAATATGGACAAGATGCCTGCAATGTTGGAGATGAGGGTGGATTCGCTCCAAATGTTCAAGATAACAGAGAAGGACTGGTGTTGCTTATTGATGCCATTGAAAAGGCTGGCTATACAGGAAAGATCAAAATAGGAATGGATGTTGCAGCTTCCGAGTTTTTCACTGCAGAGGGAAAATATGATTTGAACTTCAAGAAACAGCCAAATGATGGAGCTCATGTCCACTCAGCTCATGGCCTTGGCGAACTCTATAAACAGTTTGTCAAAGATTTTCCCATTGTTTCCATCGAGGACCCCTTTGATCAAGATGATTGGAGTTCCTGGGCTTCTCTACAATCTTCCGTTGATATCCAACTTGTGGGAGATGATTTGCTGGTAACAAATCCAAAGAGAATTGCCGAAGGCATTCAGAAAAAAGCTTGTAATGCTTTACTTCTGAAGGTTAACCAAATTGGATCAGTGACTGAATCAATTCAAGCTGCTCTTGATTCCAAGGCTGCTGGCTGGGGAGTGATGGTCAGCCACCGAAGTGGTGAAACTGAAGATAATTTTATTGCGGATCTTTCAGTTGGCTTGGCTAGTGGACAGATCAAAACTGGAGCTCCATGCCGAAGCGAACGGTTAGCCAAATATAATCAGCTTCTTCGCATTGAAGAAGAACTCGGCAGTGTTCGATATGCCGGTGCGTCTTTCAGATCACCTTAGGAGTTAAAAGTTCAAGGTGATGACTGTGTTGATGTATCAACTTTAGGCTTGATAGAAGAACATGTAGAGGATTTCTTCAGAAATAATATGCTCAACTTTTCTGTCCAAGGTGTTAGAATCTGACACGTTACGAAGTTCATTCGAGTGTTGCCATGAGAGTATTTAAATTTTTGTTATGAACTATTCGTTTCAAAATATTGCAGTGTTTTGATTCACAAACTACCTCGTGGGGTTCTCTTTTCTTCAAAGCTATTCCTTACAGCCAA

Coding sequence (CDS)

ATGGCTTCTCTTCCTTCCCCACCCACCATGAATCTCCTCAACAAATCCCTCTCTCTTTCCCCACCTCAATCCTTCATTGCCTTAATACCTTCTTCTTCTTCTTCTTCCTCCAAACCCCTTCGTTCCCCTACTATTCGCTGTTCTGTTGCTGTGGCTCCTTCTGTTTCAACCAACGTGTCCAAGGAATTCAAGTTGAAGTCCCTCAAGGCCCGTCAGATCATTGACAGTAGAGGCAATCCTACGGTCGAGGTTGATTTGATTACTGACGATCTCTACCGATCCGCTGTTCCTAGTGGGGCTTCTACTGGGATCTATGAGGCGTTGGAGCTTAGAGATGGGGACAAAACTGTTTATGGAGGTAAAGGTGTGCTTACTGCTGTTAAGAATATCAATGATATCTTGGCTCCTAAGCTCGTTGGCGTCGATGTCAGGAATCAAGAAGAAGTTGATGCAATCATGCTGGAAATTGATGGAACTCCAAACAAGTCGGAACTTGGGGCAAATGCTATACTAGGAGTTTCTTTGAGTGTCTGCAGAGCTGGTGCTGGCGCTAAGGGACTACCATTGTATAGACACATCCAAGAATTATCAGGCACCAAGGAACTTGTTATGCCAGTTCCTGCTTTTAATGTTATAAACGGGGGTAGCCATGCTGGAAATAATCTAGCAATGCAAGAATTTATGATCTTACCTGTAGGTGCTTCATCTTTCGCAGAGGCACTTCGGATGGGAAGTGAAGTTTATCACACACTAAAGGGAATTATTAAAGCAAAATATGGACAAGATGCCTGCAATGTTGGAGATGAGGGTGGATTCGCTCCAAATGTTCAAGATAACAGAGAAGGACTGGTGTTGCTTATTGATGCCATTGAAAAGGCTGGCTATACAGGAAAGATCAAAATAGGAATGGATGTTGCAGCTTCCGAGTTTTTCACTGCAGAGGGAAAATATGATTTGAACTTCAAGAAACAGCCAAATGATGGAGCTCATGTCCACTCAGCTCATGGCCTTGGCGAACTCTATAAACAGTTTGTCAAAGATTTTCCCATTGTTTCCATCGAGGACCCCTTTGATCAAGATGATTGGAGTTCCTGGGCTTCTCTACAATCTTCCGTTGATATCCAACTTGTGGGAGATGATTTGCTGGTAACAAATCCAAAGAGAATTGCCGAAGGCATTCAGAAAAAAGCTTGTAATGCTTTACTTCTGAAGGTTAACCAAATTGGATCAGTGACTGAATCAATTCAAGCTGCTCTTGATTCCAAGGCTGCTGGCTGGGGAGTGATGGTCAGCCACCGAAGTGGTGAAACTGAAGATAATTTTATTGCGGATCTTTCAGTTGGCTTGGCTAGTGGACAGATCAAAACTGGAGCTCCATGCCGAAGCGAACGGTTAGCCAAATATAATCAGCTTCTTCGCATTGAAGAAGAACTCGGCAGTGTTCGATATGCCGGTGCGTCTTTCAGATCACCTTAG

Protein sequence

MASLPSPPTMNLLNKSLSLSPPQSFIALIPSSSSSSSKPLRSPTIRCSVAVAPSVSTNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP*
Homology
BLAST of CSPI01G03020 vs. ExPASy Swiss-Prot
Match: Q9C9C4 (Enolase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ENO1 PE=1 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 1.4e-225
Identity = 391/459 (85.19%), Postives = 427/459 (93.03%), Query Frame = 0

Query: 33  SSSSSKPLRSPTIRCSVAVAPSVSTNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLY 92
           S+ S    R   ++CSV         V+KE ++K +KARQIIDSRGNPTVEVDLITDDLY
Sbjct: 28  SAPSCLRFRRSGVQCSV---------VAKECRVKGVKARQIIDSRGNPTVEVDLITDDLY 87

Query: 93  RSAVPSGASTGIYEALELRDGDKTVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAI 152
           RSAVPSGASTGIYEALELRDGDK+VYGGKGVL A+KNIN+++APKL+GVDVRNQ +VDA+
Sbjct: 88  RSAVPSGASTGIYEALELRDGDKSVYGGKGVLQAIKNINELVAPKLIGVDVRNQADVDAL 147

Query: 153 MLEIDGTPNKSELGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 212
           MLE+DGTPNKS+LGANAILGVSLSVCRAGAGAKG+PLY+HIQE SGTKELVMPVPAFNVI
Sbjct: 148 MLELDGTPNKSKLGANAILGVSLSVCRAGAGAKGVPLYKHIQETSGTKELVMPVPAFNVI 207

Query: 213 NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 272
           NGGSHAGN+LAMQEFMILPVGA+SF+EA +MGSEVYHTLKGIIK KYGQDACNVGDEGGF
Sbjct: 208 NGGSHAGNSLAMQEFMILPVGATSFSEAFQMGSEVYHTLKGIIKTKYGQDACNVGDEGGF 267

Query: 273 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVH 332
           APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFF  +G+YDLNFKKQPNDGAHV 
Sbjct: 268 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFMKDGRYDLNFKKQPNDGAHVL 327

Query: 333 SAHGLGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 392
           SA  L +LY++F+KDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAE 
Sbjct: 328 SAESLADLYREFIKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEA 387

Query: 393 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 452
           I+K++CNALLLKVNQIG+VTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG
Sbjct: 388 IKKQSCNALLLKVNQIGTVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 447

Query: 453 QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP 492
           QIKTGAPCRSERL+KYNQLLRIEEELG+VRYAG +FRSP
Sbjct: 448 QIKTGAPCRSERLSKYNQLLRIEEELGNVRYAGEAFRSP 477

BLAST of CSPI01G03020 vs. ExPASy Swiss-Prot
Match: Q54RK5 (Enolase A OS=Dictyostelium discoideum OX=44689 GN=enoA PE=3 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 1.7e-170
Identity = 302/431 (70.07%), Postives = 358/431 (83.06%), Query Frame = 0

Query: 65  LKSLKARQIIDSRGNPTVEVDLITD-----DLYRSAVPSGASTGIYEALELRDGDKTVYG 124
           +KS+KAR+I++SRGNPTVEVDL T+       +R+AVPSGASTGIYEA+ELRDGDK+ Y 
Sbjct: 4   IKSIKAREILNSRGNPTVEVDLYTEKDGVVSSFRAAVPSGASTGIYEAVELRDGDKSRYL 63

Query: 125 GKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCR 184
           GKGVL A+KNI +++ P ++G  V +Q  +D +M+++DGTPNK +LGANAIL VSL+VCR
Sbjct: 64  GKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGTPNKGKLGANAILAVSLAVCR 123

Query: 185 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAE 244
           AGA  + LPLY++I E++GTK + +PVPAFNVINGGSHAGN LAMQEFMILPVGA  F E
Sbjct: 124 AGAADRNLPLYKYISEIAGTK-MRLPVPAFNVINGGSHAGNKLAMQEFMILPVGAKDFNE 183

Query: 245 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 304
           A RMGSEVYH LK +I  +YGQDA NVGDEGGFAP +Q N+EGL LL  AIEKAGYTG +
Sbjct: 184 AYRMGSEVYHNLKNVISGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLV 243

Query: 305 KIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ 364
           KIGMD AASE F  E  YDL+FK + NDG+ V S   LG+LY++F+K++PI+SIEDPFDQ
Sbjct: 244 KIGMDCAASE-FKVENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQ 303

Query: 365 DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAAL 424
           DDW S+  L +SVDIQ+VGDDLLVTNP+RI  GI+KKACNALLLKVNQIGSVTESI+AAL
Sbjct: 304 DDWESYTKLTASVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAAL 363

Query: 425 DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG 484
           DSK A WGVMVSHRSGETED FIADL VGL +GQIKTGAPCRSERLAKYNQL+RI EELG
Sbjct: 364 DSKNASWGVMVSHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELG 423

Query: 485 -SVRYAGASFR 490
            +  YAG +FR
Sbjct: 424 ENHNYAGLTFR 432

BLAST of CSPI01G03020 vs. ExPASy Swiss-Prot
Match: P15007 (Enolase OS=Drosophila melanogaster OX=7227 GN=Eno PE=1 SV=2)

HSP 1 Score: 595.1 bits (1533), Expect = 7.1e-169
Identity = 297/440 (67.50%), Postives = 360/440 (81.82%), Query Frame = 0

Query: 56  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITD-DLYRSAVPSGASTGIYEALELRDGD 115
           ST  S E  +K++KARQI DSRGNPTVEVDL T+  L+R+AVPSGASTG++EALELRD D
Sbjct: 61  STCDSNEMTIKAIKARQIYDSRGNPTVEVDLTTELGLFRAAVPSGASTGVHEALELRDND 120

Query: 116 KTVYGGKGVLTAVKNINDILAPKLV--GVDVRNQEEVDAIMLEIDGTPNKSELGANAILG 175
           K  Y GK VL AV ++ND L P+L+   +DV +Q  +D  M+++DGT NKS+ GANAILG
Sbjct: 121 KANYHGKSVLKAVGHVNDTLGPELIKANLDVVDQASIDNFMIKLDGTENKSKFGANAILG 180

Query: 176 VSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPV 235
           VSL+V +AGA  KG+PLY+HI +L+G KE+++PVPAFNVINGGSHAGN LAMQEFMILP 
Sbjct: 181 VSLAVAKAGAAKKGVPLYKHIADLAGNKEIILPVPAFNVINGGSHAGNKLAMQEFMILPT 240

Query: 236 GASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEK 295
           GA+SF EA++MGSEVYH LK +IKAK+G DA  VGDEGGFAPN+Q N+E L L+ DAI K
Sbjct: 241 GATSFTEAMKMGSEVYHHLKNVIKAKFGLDATAVGDEGGFAPNIQSNKEALNLISDAIAK 300

Query: 296 AGYTGKIKIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVS 355
           AGYTGKI+IGMDVAASEF+  +G+YDL+FK + +D +    A  L  LY++F+KDFPIVS
Sbjct: 301 AGYTGKIEIGMDVAASEFY-KDGQYDLDFKNEKSDKSQWLPADKLANLYQEFIKDFPIVS 360

Query: 356 IEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVT 415
           IEDPFDQD W +W++L    DIQ+VGDDL VTNPKRIA  ++KKACN LLLKVNQIG+VT
Sbjct: 361 IEDPFDQDHWEAWSNLTGCTDIQIVGDDLTVTNPKRIATAVEKKACNCLLLKVNQIGTVT 420

Query: 416 ESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLL 475
           ESI A L +K  GWG MVSHRSGETED+FI DL VGL++GQIKTGAPCRSERLAKYNQ+L
Sbjct: 421 ESIAAHLLAKKNGWGTMVSHRSGETEDSFIGDLVVGLSTGQIKTGAPCRSERLAKYNQIL 480

Query: 476 RIEEELGS-VRYAGASFRSP 492
           RIEEE+G+ V++AG SFR P
Sbjct: 481 RIEEEIGAGVKFAGKSFRKP 499

BLAST of CSPI01G03020 vs. ExPASy Swiss-Prot
Match: P15429 (Beta-enolase OS=Rattus norvegicus OX=10116 GN=Eno3 PE=1 SV=3)

HSP 1 Score: 591.7 bits (1524), Expect = 7.9e-168
Identity = 302/431 (70.07%), Postives = 355/431 (82.37%), Query Frame = 0

Query: 65  LKSLKARQIIDSRGNPTVEVDLIT-DDLYRSAVPSGASTGIYEALELRDGDKTVYGGKGV 124
           ++ + AR+I+DSRGNPTVEVDL T    +R+AVPSGASTGIYEALELRDGDK+ Y GKGV
Sbjct: 3   MQKIFAREILDSRGNPTVEVDLHTAKGRFRAAVPSGASTGIYEALELRDGDKSRYLGKGV 62

Query: 125 LTAVKNINDILAPKLV--GVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCRAG 184
           L AV++IN  L P L+   + V +QE+VD  M+E+DGT NKS+ GANAILGVSL+VC+AG
Sbjct: 63  LKAVEHINKTLGPALLEKKLSVVDQEKVDKFMIELDGTENKSKFGANAILGVSLAVCKAG 122

Query: 185 AGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAEAL 244
           A  KG+PLYRHI +L+G  +LV+PVPAFNVINGGSHAGN LAMQEFMILPVGASSF EA+
Sbjct: 123 AAEKGVPLYRHIADLAGNPDLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAM 182

Query: 245 RMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKI 304
           R+G+EVYH LKG+IKAKYG+DA NVGDEGGFAPN+ +N E L LL  AI+ AGY  K+ I
Sbjct: 183 RIGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIQAAGYPDKVVI 242

Query: 305 GMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQDD 364
           GMDVAASEF+   GKYDL+F K P+D A   S   LGELYK F+K++P+VSIEDPFDQDD
Sbjct: 243 GMDVAASEFY-RNGKYDLDF-KSPDDPARHISGEKLGELYKSFIKNYPVVSIEDPFDQDD 302

Query: 365 WSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAALDS 424
           W++W S  S VDIQ+VGDDL VTNPKRIA+ ++KKACN LLLKVNQIGSVTESIQA   +
Sbjct: 303 WATWTSFLSGVDIQIVGDDLTVTNPKRIAQAVEKKACNCLLLKVNQIGSVTESIQACKLA 362

Query: 425 KAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG-S 484
           ++ GWGVMVSHRSGETED FIADL VGL +GQIKTGAPCRSERLAKYNQL+RIEE LG  
Sbjct: 363 QSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDK 422

Query: 485 VRYAGASFRSP 492
             +AG  FR+P
Sbjct: 423 AVFAGRKFRNP 431

BLAST of CSPI01G03020 vs. ExPASy Swiss-Prot
Match: Q27527 (Enolase OS=Caenorhabditis elegans OX=6239 GN=enol-1 PE=1 SV=3)

HSP 1 Score: 591.3 bits (1523), Expect = 1.0e-167
Identity = 303/431 (70.30%), Postives = 352/431 (81.67%), Query Frame = 0

Query: 65  LKSLKARQIIDSRGNPTVEVDLITD-DLYRSAVPSGASTGIYEALELRDGDKTVYGGKGV 124
           +  + ARQI DSRGNPTVEVDL T+  ++R+AVPSGASTG++EALELRDGDK V+ GKGV
Sbjct: 3   ITKIHARQIYDSRGNPTVEVDLFTEKGVFRAAVPSGASTGVHEALELRDGDKAVHLGKGV 62

Query: 125 LTAVKNINDILAPKLV--GVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCRAG 184
           L AV NIN+ +AP L+  G DV  Q+++D  M+ +DG+ NK  LGANAILGVSL+V +AG
Sbjct: 63  LKAVSNINEKIAPALIAKGFDVTAQKDIDDFMMALDGSENKGNLGANAILGVSLAVAKAG 122

Query: 185 AGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAEAL 244
           A  KGLPLY++I EL+GT ++V+PVPAFNVINGGSHAGN LAMQEFMILPVGASSFAEA+
Sbjct: 123 AVHKGLPLYKYIAELAGTGKVVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFAEAM 182

Query: 245 RMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKI 304
           RMGSEVYH LK  IK +YG DA  VGDEGGFAPN+QDN+EGL LL  AI+KAGYTGKI I
Sbjct: 183 RMGSEVYHHLKAEIKKRYGLDATAVGDEGGFAPNIQDNKEGLDLLNTAIDKAGYTGKISI 242

Query: 305 GMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQDD 364
           GMDVAASEFF  +GKYDL+FK   +D +   S   L ELY+ F+K++P+VSIED FDQDD
Sbjct: 243 GMDVAASEFF-KDGKYDLDFKNPASDSSKWLSGEQLTELYQSFIKEYPVVSIEDAFDQDD 302

Query: 365 WSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAALDS 424
           W +W     +  IQLVGDDL VTNPKRI   I KK+CN LLLKVNQIGSVTESI+AA  S
Sbjct: 303 WDNWGKFHGATSIQLVGDDLTVTNPKRIQTAIDKKSCNCLLLKVNQIGSVTESIEAAKLS 362

Query: 425 KAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGS- 484
           +A GWGVMVSHRSGETED FIADL VGLA+GQIKTGAPCRSERLAKYNQLLRIEEELG+ 
Sbjct: 363 RANGWGVMVSHRSGETEDTFIADLVVGLATGQIKTGAPCRSERLAKYNQLLRIEEELGAD 422

Query: 485 VRYAGASFRSP 492
             YAG +FR+P
Sbjct: 423 AVYAGHNFRNP 432

BLAST of CSPI01G03020 vs. ExPASy TrEMBL
Match: A0A0A0LPK6 (Phosphopyruvate hydratase OS=Cucumis sativus OX=3659 GN=Csa_1G014400 PE=3 SV=1)

HSP 1 Score: 949.1 bits (2452), Expect = 7.2e-273
Identity = 490/492 (99.59%), Postives = 490/492 (99.59%), Query Frame = 0

Query: 1   MASLPSPPTMNLLNKSLSLSPPQSFIALIP-SSSSSSSKPLRSPTIRCSVAVAPSVSTNV 60
           MASLPSP TMNLLNKSLSLSPPQSFIALIP SSSSSSSKPLRSPTIRCSVAVAPSVSTNV
Sbjct: 1   MASLPSPHTMNLLNKSLSLSPPQSFIALIPSSSSSSSSKPLRSPTIRCSVAVAPSVSTNV 60

Query: 61  SKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYG 120
           SKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYG
Sbjct: 61  SKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYG 120

Query: 121 GKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCR 180
           GKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCR
Sbjct: 121 GKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCR 180

Query: 181 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAE 240
           AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAE
Sbjct: 181 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAE 240

Query: 241 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 300
           ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI
Sbjct: 241 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 300

Query: 301 KIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ 360
           KIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ
Sbjct: 301 KIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ 360

Query: 361 DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAAL 420
           DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAAL
Sbjct: 361 DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAAL 420

Query: 421 DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG 480
           DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG
Sbjct: 421 DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG 480

Query: 481 SVRYAGASFRSP 492
           SVRYAGASFRSP
Sbjct: 481 SVRYAGASFRSP 492

BLAST of CSPI01G03020 vs. ExPASy TrEMBL
Match: A0A5A7US84 (Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003390 PE=3 SV=1)

HSP 1 Score: 937.2 bits (2421), Expect = 2.8e-269
Identity = 484/496 (97.58%), Postives = 485/496 (97.78%), Query Frame = 0

Query: 1   MASLPSPPTMNLLNKSLSLSPPQSFIALIP-----SSSSSSSKPLRSPTIRCSVAVAPSV 60
           MASLPSPPTMNLLNK LSLSPPQSFI  IP     SSSSSSSKPLRSPTIRCSVAVAPSV
Sbjct: 1   MASLPSPPTMNLLNKPLSLSPPQSFIVSIPSSSSSSSSSSSSKPLRSPTIRCSVAVAPSV 60

Query: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120
           STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK
Sbjct: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120

Query: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSL 180
           TVYGGKGVLTAVKNI DILAPKLVGVDVRNQEEVDAIMLEIDGTPNKS+LGANAILGVSL
Sbjct: 121 TVYGGKGVLTAVKNIKDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180

Query: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240
           SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS
Sbjct: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240

Query: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300
           SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY
Sbjct: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300

Query: 301 TGKIKIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIED 360
           TGKIKIGMDVAASEFFT EGKYDLNFKKQPNDGAHVHSAH LGELYKQFVKDFPIVSIED
Sbjct: 301 TGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHALGELYKQFVKDFPIVSIED 360

Query: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420
           PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI
Sbjct: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420

Query: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480
           QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE
Sbjct: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480

Query: 481 EELGSVRYAGASFRSP 492
           EELGSVRYAGASFRSP
Sbjct: 481 EELGSVRYAGASFRSP 496

BLAST of CSPI01G03020 vs. ExPASy TrEMBL
Match: A0A1S3BX56 (Phosphopyruvate hydratase OS=Cucumis melo OX=3656 GN=LOC103494088 PE=3 SV=1)

HSP 1 Score: 937.2 bits (2421), Expect = 2.8e-269
Identity = 484/496 (97.58%), Postives = 485/496 (97.78%), Query Frame = 0

Query: 1   MASLPSPPTMNLLNKSLSLSPPQSFIALIP-----SSSSSSSKPLRSPTIRCSVAVAPSV 60
           MASLPSPPTMNLLNK LSLSPPQSFI  IP     SSSSSSSKPLRSPTIRCSVAVAPSV
Sbjct: 1   MASLPSPPTMNLLNKPLSLSPPQSFIVSIPSSSSSSSSSSSSKPLRSPTIRCSVAVAPSV 60

Query: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120
           STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK
Sbjct: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120

Query: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSL 180
           TVYGGKGVLTAVKNI DILAPKLVGVDVRNQEEVDAIMLEIDGTPNKS+LGANAILGVSL
Sbjct: 121 TVYGGKGVLTAVKNIKDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180

Query: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240
           SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS
Sbjct: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240

Query: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300
           SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY
Sbjct: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300

Query: 301 TGKIKIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIED 360
           TGKIKIGMDVAASEFFT EGKYDLNFKKQPNDGAHVHSAH LGELYKQFVKDFPIVSIED
Sbjct: 301 TGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHALGELYKQFVKDFPIVSIED 360

Query: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420
           PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI
Sbjct: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420

Query: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480
           QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE
Sbjct: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480

Query: 481 EELGSVRYAGASFRSP 492
           EELGSVRYAGASFRSP
Sbjct: 481 EELGSVRYAGASFRSP 496

BLAST of CSPI01G03020 vs. ExPASy TrEMBL
Match: A0A5D3DYS6 (Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00060 PE=3 SV=1)

HSP 1 Score: 936.8 bits (2420), Expect = 3.7e-269
Identity = 484/496 (97.58%), Postives = 485/496 (97.78%), Query Frame = 0

Query: 1   MASLPSPPTMNLLNKSLSLSPPQSFIALIP-----SSSSSSSKPLRSPTIRCSVAVAPSV 60
           MASLPSPPTMNLLNK LSLSPPQSFI  IP     SSSSSSSKPLRSPTIRCSVAVAPSV
Sbjct: 1   MASLPSPPTMNLLNKPLSLSPPQSFIVSIPSSSSSSSSSSSSKPLRSPTIRCSVAVAPSV 60

Query: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120
           STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK
Sbjct: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120

Query: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSL 180
           TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKS+LGANAILGVSL
Sbjct: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180

Query: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240
           SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS
Sbjct: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240

Query: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300
           SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY
Sbjct: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300

Query: 301 TGKIKIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIED 360
           TGKIKIGMDVAASEFFT  GKYDLNFKKQPNDGAHVHSAH LGELYKQFVKDFPIVSIED
Sbjct: 301 TGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVHSAHALGELYKQFVKDFPIVSIED 360

Query: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420
           PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI
Sbjct: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420

Query: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480
           QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE
Sbjct: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480

Query: 481 EELGSVRYAGASFRSP 492
           EELGSVRYAGASFRSP
Sbjct: 481 EELGSVRYAGASFRSP 496

BLAST of CSPI01G03020 vs. ExPASy TrEMBL
Match: A0A6J1EV05 (Phosphopyruvate hydratase OS=Cucurbita moschata OX=3662 GN=LOC111437858 PE=3 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 1.1e-252
Identity = 457/493 (92.70%), Postives = 467/493 (94.73%), Query Frame = 0

Query: 1   MASLPSPP--TMNLLNKSLSLSPPQSFIALIPSSSSSSSKPLRSPTIRCSVAVAPSVSTN 60
           MASLPSPP  T NLLNKSL  S PQS I  IP  SSSSSKPLRS  +RCSVAVAPS ST 
Sbjct: 1   MASLPSPPTTTTNLLNKSLLPSKPQSPIISIP--SSSSSKPLRSHGVRCSVAVAPSASTK 60

Query: 61  VSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVY 120
            S EFKLKS+KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK VY
Sbjct: 61  ASSEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNVY 120

Query: 121 GGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVC 180
           GGKGVLTAVKNINDILAPKLVGVDVRNQE+VDAIMLEIDGT NKS+LGANAILGVSLSVC
Sbjct: 121 GGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVC 180

Query: 181 RAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFA 240
           RAGAGAKGLPLYRHIQELS TKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA+SFA
Sbjct: 181 RAGAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFA 240

Query: 241 EALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGK 300
           EALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLL DAIEKAGYTGK
Sbjct: 241 EALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGK 300

Query: 301 IKIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFD 360
           IKIGMDVAASEFFT +G+YDLNFKKQPNDGAHVHSAHGLGELYKQFV DFPIVSIEDPFD
Sbjct: 301 IKIGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFD 360

Query: 361 QDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAA 420
           QDDWSSWASLQSSV +Q+VGDDLLVTNPKRIAE IQKKACNALLLKVNQIGSVTESIQAA
Sbjct: 361 QDDWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAA 420

Query: 421 LDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEEL 480
           LDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEEL
Sbjct: 421 LDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEEL 480

Query: 481 GSVRYAGASFRSP 492
           G+VRYAG SFRSP
Sbjct: 481 GNVRYAGESFRSP 491

BLAST of CSPI01G03020 vs. NCBI nr
Match: XP_004138202.1 (enolase 1, chloroplastic [Cucumis sativus] >KGN63743.1 hypothetical protein Csa_014309 [Cucumis sativus])

HSP 1 Score: 949.1 bits (2452), Expect = 1.5e-272
Identity = 490/492 (99.59%), Postives = 490/492 (99.59%), Query Frame = 0

Query: 1   MASLPSPPTMNLLNKSLSLSPPQSFIALIP-SSSSSSSKPLRSPTIRCSVAVAPSVSTNV 60
           MASLPSP TMNLLNKSLSLSPPQSFIALIP SSSSSSSKPLRSPTIRCSVAVAPSVSTNV
Sbjct: 1   MASLPSPHTMNLLNKSLSLSPPQSFIALIPSSSSSSSSKPLRSPTIRCSVAVAPSVSTNV 60

Query: 61  SKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYG 120
           SKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYG
Sbjct: 61  SKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYG 120

Query: 121 GKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCR 180
           GKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCR
Sbjct: 121 GKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCR 180

Query: 181 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAE 240
           AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAE
Sbjct: 181 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAE 240

Query: 241 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 300
           ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI
Sbjct: 241 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 300

Query: 301 KIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ 360
           KIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ
Sbjct: 301 KIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ 360

Query: 361 DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAAL 420
           DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAAL
Sbjct: 361 DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAAL 420

Query: 421 DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG 480
           DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG
Sbjct: 421 DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG 480

Query: 481 SVRYAGASFRSP 492
           SVRYAGASFRSP
Sbjct: 481 SVRYAGASFRSP 492

BLAST of CSPI01G03020 vs. NCBI nr
Match: XP_008453338.1 (PREDICTED: enolase 1, chloroplastic [Cucumis melo] >KAA0058034.1 enolase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 937.2 bits (2421), Expect = 5.8e-269
Identity = 484/496 (97.58%), Postives = 485/496 (97.78%), Query Frame = 0

Query: 1   MASLPSPPTMNLLNKSLSLSPPQSFIALIP-----SSSSSSSKPLRSPTIRCSVAVAPSV 60
           MASLPSPPTMNLLNK LSLSPPQSFI  IP     SSSSSSSKPLRSPTIRCSVAVAPSV
Sbjct: 1   MASLPSPPTMNLLNKPLSLSPPQSFIVSIPSSSSSSSSSSSSKPLRSPTIRCSVAVAPSV 60

Query: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120
           STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK
Sbjct: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120

Query: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSL 180
           TVYGGKGVLTAVKNI DILAPKLVGVDVRNQEEVDAIMLEIDGTPNKS+LGANAILGVSL
Sbjct: 121 TVYGGKGVLTAVKNIKDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180

Query: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240
           SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS
Sbjct: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240

Query: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300
           SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY
Sbjct: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300

Query: 301 TGKIKIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIED 360
           TGKIKIGMDVAASEFFT EGKYDLNFKKQPNDGAHVHSAH LGELYKQFVKDFPIVSIED
Sbjct: 301 TGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHALGELYKQFVKDFPIVSIED 360

Query: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420
           PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI
Sbjct: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420

Query: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480
           QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE
Sbjct: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480

Query: 481 EELGSVRYAGASFRSP 492
           EELGSVRYAGASFRSP
Sbjct: 481 EELGSVRYAGASFRSP 496

BLAST of CSPI01G03020 vs. NCBI nr
Match: TYK28380.1 (enolase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 936.8 bits (2420), Expect = 7.6e-269
Identity = 484/496 (97.58%), Postives = 485/496 (97.78%), Query Frame = 0

Query: 1   MASLPSPPTMNLLNKSLSLSPPQSFIALIP-----SSSSSSSKPLRSPTIRCSVAVAPSV 60
           MASLPSPPTMNLLNK LSLSPPQSFI  IP     SSSSSSSKPLRSPTIRCSVAVAPSV
Sbjct: 1   MASLPSPPTMNLLNKPLSLSPPQSFIVSIPSSSSSSSSSSSSKPLRSPTIRCSVAVAPSV 60

Query: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120
           STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK
Sbjct: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120

Query: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSL 180
           TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKS+LGANAILGVSL
Sbjct: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180

Query: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240
           SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS
Sbjct: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240

Query: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300
           SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY
Sbjct: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300

Query: 301 TGKIKIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIED 360
           TGKIKIGMDVAASEFFT  GKYDLNFKKQPNDGAHVHSAH LGELYKQFVKDFPIVSIED
Sbjct: 301 TGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVHSAHALGELYKQFVKDFPIVSIED 360

Query: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420
           PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI
Sbjct: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420

Query: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480
           QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE
Sbjct: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480

Query: 481 EELGSVRYAGASFRSP 492
           EELGSVRYAGASFRSP
Sbjct: 481 EELGSVRYAGASFRSP 496

BLAST of CSPI01G03020 vs. NCBI nr
Match: XP_038879680.1 (enolase 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 917.5 bits (2370), Expect = 4.8e-263
Identity = 472/491 (96.13%), Postives = 479/491 (97.56%), Query Frame = 0

Query: 1   MASLPSPPTMNLLNKSLSLSPPQSFIALIPSSSSSSSKPLRSPTIRCSVAVAPSVSTNVS 60
           MASLPSPPTMNLL KSL  S PQSFI+ IP SSSSSSKPLRS  IRCSVAVAPSVST  S
Sbjct: 1   MASLPSPPTMNLLEKSLLPSSPQSFISSIP-SSSSSSKPLRSHAIRCSVAVAPSVSTKAS 60

Query: 61  KEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYGG 120
           KEFKLKS+KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYGG
Sbjct: 61  KEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYGG 120

Query: 121 KGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCRA 180
           KGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKS+LGANAILGVSLSVCRA
Sbjct: 121 KGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSLSVCRA 180

Query: 181 GAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAEA 240
           GAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA SFAEA
Sbjct: 181 GAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAISFAEA 240

Query: 241 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIK 300
           LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDN+EGLVLLIDAIEKAGYTGKIK
Sbjct: 241 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNKEGLVLLIDAIEKAGYTGKIK 300

Query: 301 IGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQD 360
           IGMDVAASEFFT EGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQD
Sbjct: 301 IGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQD 360

Query: 361 DWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAALD 420
           DWSSWASLQSSVDIQLVGDDLLVTNPKRIA+GIQKKACNALLLKVNQIGSVTESIQAALD
Sbjct: 361 DWSSWASLQSSVDIQLVGDDLLVTNPKRIAKGIQKKACNALLLKVNQIGSVTESIQAALD 420

Query: 421 SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGS 480
           SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEE+GS
Sbjct: 421 SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEEIGS 480

Query: 481 VRYAGASFRSP 492
           +RYAGASFRSP
Sbjct: 481 IRYAGASFRSP 490

BLAST of CSPI01G03020 vs. NCBI nr
Match: KAG6587623.1 (Enolase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 882.9 bits (2280), Expect = 1.3e-252
Identity = 457/493 (92.70%), Postives = 468/493 (94.93%), Query Frame = 0

Query: 1   MASLPSPP--TMNLLNKSLSLSPPQSFIALIPSSSSSSSKPLRSPTIRCSVAVAPSVSTN 60
           MASLPSPP  T NLLNKSL  S PQS I  IP  SSSSSKPLRS  +RCSVAVAPS ST 
Sbjct: 1   MASLPSPPTTTTNLLNKSLLPSKPQSPIISIP--SSSSSKPLRSHGVRCSVAVAPSASTK 60

Query: 61  VSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVY 120
            S+EFKLKS+KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK VY
Sbjct: 61  ASREFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNVY 120

Query: 121 GGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVC 180
           GGKGVLTAVKNINDILAPKLVGVDVRNQE+VDAIMLEIDGT NKS+LGANAILGVSLSVC
Sbjct: 121 GGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVC 180

Query: 181 RAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFA 240
           RAGAGAKGLPLYRHIQELS TKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA+SFA
Sbjct: 181 RAGAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFA 240

Query: 241 EALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGK 300
           EALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLL DAIEKAGYTGK
Sbjct: 241 EALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGK 300

Query: 301 IKIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFD 360
           IKIGMDVAASEFFT +G+YDLNFKKQPNDGAHVHSAHGLGELYKQFV DFPIVSIEDPFD
Sbjct: 301 IKIGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFD 360

Query: 361 QDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAA 420
           QDDWSSWASLQSSV +Q+VGDDLLVTNPKRIAE IQKKACNALLLKVNQIGSVTESIQAA
Sbjct: 361 QDDWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAA 420

Query: 421 LDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEEL 480
           LDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEEL
Sbjct: 421 LDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEEL 480

Query: 481 GSVRYAGASFRSP 492
           G+VRYAG SFRSP
Sbjct: 481 GNVRYAGESFRSP 491

BLAST of CSPI01G03020 vs. TAIR 10
Match: AT1G74030.1 (enolase 1 )

HSP 1 Score: 783.5 bits (2022), Expect = 1.0e-226
Identity = 391/459 (85.19%), Postives = 427/459 (93.03%), Query Frame = 0

Query: 33  SSSSSKPLRSPTIRCSVAVAPSVSTNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLY 92
           S+ S    R   ++CSV         V+KE ++K +KARQIIDSRGNPTVEVDLITDDLY
Sbjct: 28  SAPSCLRFRRSGVQCSV---------VAKECRVKGVKARQIIDSRGNPTVEVDLITDDLY 87

Query: 93  RSAVPSGASTGIYEALELRDGDKTVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAI 152
           RSAVPSGASTGIYEALELRDGDK+VYGGKGVL A+KNIN+++APKL+GVDVRNQ +VDA+
Sbjct: 88  RSAVPSGASTGIYEALELRDGDKSVYGGKGVLQAIKNINELVAPKLIGVDVRNQADVDAL 147

Query: 153 MLEIDGTPNKSELGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 212
           MLE+DGTPNKS+LGANAILGVSLSVCRAGAGAKG+PLY+HIQE SGTKELVMPVPAFNVI
Sbjct: 148 MLELDGTPNKSKLGANAILGVSLSVCRAGAGAKGVPLYKHIQETSGTKELVMPVPAFNVI 207

Query: 213 NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 272
           NGGSHAGN+LAMQEFMILPVGA+SF+EA +MGSEVYHTLKGIIK KYGQDACNVGDEGGF
Sbjct: 208 NGGSHAGNSLAMQEFMILPVGATSFSEAFQMGSEVYHTLKGIIKTKYGQDACNVGDEGGF 267

Query: 273 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVH 332
           APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFF  +G+YDLNFKKQPNDGAHV 
Sbjct: 268 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFMKDGRYDLNFKKQPNDGAHVL 327

Query: 333 SAHGLGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 392
           SA  L +LY++F+KDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAE 
Sbjct: 328 SAESLADLYREFIKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEA 387

Query: 393 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 452
           I+K++CNALLLKVNQIG+VTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG
Sbjct: 388 IKKQSCNALLLKVNQIGTVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 447

Query: 453 QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP 492
           QIKTGAPCRSERL+KYNQLLRIEEELG+VRYAG +FRSP
Sbjct: 448 QIKTGAPCRSERLSKYNQLLRIEEELGNVRYAGEAFRSP 477

BLAST of CSPI01G03020 vs. TAIR 10
Match: AT2G36530.1 (Enolase )

HSP 1 Score: 575.1 bits (1481), Expect = 5.4e-164
Identity = 295/435 (67.82%), Postives = 349/435 (80.23%), Query Frame = 0

Query: 68  LKARQIIDSRGNPTVEVDLITDDLYR--SAVPSGASTGIYEALELRDGDKTVYGGKGVLT 127
           +KARQI DSRGNPTVEVD+ T +  +  +AVPSGASTGIYEALELRDG    Y GKGV  
Sbjct: 7   VKARQIFDSRGNPTVEVDIHTSNGIKVTAAVPSGASTGIYEALELRDGGSD-YLGKGVSK 66

Query: 128 AVKNINDILAPKLVGVDVRNQEEVDAIML-EIDGTPN-----KSELGANAILGVSLSVCR 187
           AV N+N+I+ P L+G D   Q  +D  M+ E+DGT N     K +LGANAIL VSL+VC+
Sbjct: 67  AVGNVNNIIGPALIGKDPTQQTAIDNFMVHELDGTQNEWGWCKQKLGANAILAVSLAVCK 126

Query: 188 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAE 247
           AGA   G+PLY+HI  L+G  ++V+PVPAFNVINGGSHAGN LAMQEFMILPVGA+SF E
Sbjct: 127 AGAVVSGIPLYKHIANLAGNPKIVLPVPAFNVINGGSHAGNKLAMQEFMILPVGAASFKE 186

Query: 248 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 307
           A++MG EVYH LK +IK KYGQDA NVGDEGGFAPN+Q+N+EGL LL  AIEKAGYTGK+
Sbjct: 187 AMKMGVEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIEKAGYTGKV 246

Query: 308 KIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ 367
            IGMDVAASEF++ +  YDLNFK++ N+G+   S   L +LYK FV ++PIVSIEDPFDQ
Sbjct: 247 VIGMDVAASEFYSEDKTYDLNFKEENNNGSQKISGDALKDLYKSFVAEYPIVSIEDPFDQ 306

Query: 368 DDWSSWASL--QSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQA 427
           DDW  +A +  +   ++Q+VGDDLLVTNPKR+A+ I +K+CNALLLKVNQIGSVTESI+A
Sbjct: 307 DDWEHYAKMTTECGTEVQIVGDDLLVTNPKRVAKAIAEKSCNALLLKVNQIGSVTESIEA 366

Query: 428 ALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEE 487
              SK AGWGVM SHRSGETED FIADL+VGL++GQIKTGAPCRSERLAKYNQLLRIEEE
Sbjct: 367 VKMSKKAGWGVMTSHRSGETEDTFIADLAVGLSTGQIKTGAPCRSERLAKYNQLLRIEEE 426

Query: 488 LGS-VRYAGASFRSP 492
           LGS   YAG +FR P
Sbjct: 427 LGSEAIYAGVNFRKP 440

BLAST of CSPI01G03020 vs. TAIR 10
Match: AT2G29560.1 (cytosolic enolase )

HSP 1 Score: 464.5 bits (1194), Expect = 1.0e-130
Identity = 233/427 (54.57%), Postives = 307/427 (71.90%), Query Frame = 0

Query: 65  LKSLKARQIIDSRGNPTVEVDLITD-DLYRSAVPSGASTGIYEALELRDGDKTVYGGKGV 124
           +  +KARQI+DSRG PTVEVDL T+  ++R++VPSG S+G YEA+ELRDGDK +Y G  V
Sbjct: 47  ITKVKARQILDSRGIPTVEVDLHTNKGVFRASVPSGDSSGTYEAIELRDGDKGMYLGNSV 106

Query: 125 LTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSELGANAILGVSLSVCRAGAG 184
             AVKNIN+ ++  L+G+D + Q ++D  M+++D T  KSELGANAIL VS++ C+AGA 
Sbjct: 107 AKAVKNINEKISEALIGMDPKLQGQIDQAMIDLDKTEKKSELGANAILAVSIAACKAGAA 166

Query: 185 AKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAEALRM 244
            K +PL +H+ +LSG   +V+PVPAF V++GG HA N  A+QE MILP+GAS F EAL+ 
Sbjct: 167 EKEVPLCKHLSDLSGRANMVLPVPAFTVLSGGKHASNTFAIQEIMILPIGASRFEEALQW 226

Query: 245 GSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGM 304
           GSE YH LK +I  K G   CNVG++GG AP++   +EGL L+ +AI + GY  KIKI +
Sbjct: 227 GSETYHHLKAVISEKNGGLGCNVGEDGGLAPDISSLKEGLELVKEAINRTGYNDKIKIAI 286

Query: 305 DVAASEFFTAEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQDDWS 364
           D+AA+ F     KYDL+ K     G +  SA  + ++YK+   D+PIVSIEDPFD++DW 
Sbjct: 287 DIAATNFCLGT-KYDLDIKSPNKSGQNFKSAEDMIDMYKEICNDYPIVSIEDPFDKEDWE 346

Query: 365 SWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAALDSKA 424
                 S    Q+VGDDLL++N KR+   IQ+ +CNALLLKVNQIG+VTE+I+    ++ 
Sbjct: 347 HTKYFSSLGICQVVGDDLLMSNSKRVERAIQESSCNALLLKVNQIGTVTEAIEVVKMARD 406

Query: 425 AGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG-SVR 484
           A WGV+ SHR GETED+FI+DLSVGLA+G IK GAPCR ER  KYNQLLRIEEELG    
Sbjct: 407 AQWGVVTSHRCGETEDSFISDLSVGLATGVIKAGAPCRGERTMKYNQLLRIEEELGDQAV 466

Query: 485 YAGASFR 490
           YAG  ++
Sbjct: 467 YAGEDWK 472

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C9C41.4e-22585.19Enolase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ENO1 PE=1 SV=1[more]
Q54RK51.7e-17070.07Enolase A OS=Dictyostelium discoideum OX=44689 GN=enoA PE=3 SV=1[more]
P150077.1e-16967.50Enolase OS=Drosophila melanogaster OX=7227 GN=Eno PE=1 SV=2[more]
P154297.9e-16870.07Beta-enolase OS=Rattus norvegicus OX=10116 GN=Eno3 PE=1 SV=3[more]
Q275271.0e-16770.30Enolase OS=Caenorhabditis elegans OX=6239 GN=enol-1 PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A0A0LPK67.2e-27399.59Phosphopyruvate hydratase OS=Cucumis sativus OX=3659 GN=Csa_1G014400 PE=3 SV=1[more]
A0A5A7US842.8e-26997.58Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... [more]
A0A1S3BX562.8e-26997.58Phosphopyruvate hydratase OS=Cucumis melo OX=3656 GN=LOC103494088 PE=3 SV=1[more]
A0A5D3DYS63.7e-26997.58Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A6J1EV051.1e-25292.70Phosphopyruvate hydratase OS=Cucurbita moschata OX=3662 GN=LOC111437858 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
XP_004138202.11.5e-27299.59enolase 1, chloroplastic [Cucumis sativus] >KGN63743.1 hypothetical protein Csa_... [more]
XP_008453338.15.8e-26997.58PREDICTED: enolase 1, chloroplastic [Cucumis melo] >KAA0058034.1 enolase 1 [Cucu... [more]
TYK28380.17.6e-26997.58enolase 1 [Cucumis melo var. makuwa][more]
XP_038879680.14.8e-26396.13enolase 1, chloroplastic [Benincasa hispida][more]
KAG6587623.11.3e-25292.70Enolase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT1G74030.11.0e-22685.19enolase 1 [more]
AT2G36530.15.4e-16467.82Enolase [more]
AT2G29560.11.0e-13054.57cytosolic enolase [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000941EnolasePRINTSPR00148ENOLASEcoord: 166..182
score: 76.74
coord: 401..415
score: 80.2
coord: 378..389
score: 78.16
coord: 96..110
score: 88.38
coord: 223..236
score: 77.06
coord: 430..447
score: 83.0
IPR000941EnolaseTIGRFAMTIGR01060TIGR01060coord: 66..487
e-value: 2.3E-166
score: 551.6
IPR000941EnolaseSFLDSFLDF00002enolasecoord: 66..478
e-value: 0.0
score: 623.6
IPR000941EnolasePANTHERPTHR11902ENOLASEcoord: 29..491
IPR000941EnolaseHAMAPMF_00318Enolasecoord: 63..478
score: 43.597141
IPR000941EnolaseCDDcd03313enolasecoord: 67..477
e-value: 0.0
score: 741.221
IPR020811Enolase, N-terminalSMARTSM01193Enolase_N_3coord: 65..193
e-value: 3.8E-84
score: 295.5
IPR020811Enolase, N-terminalPFAMPF03952Enolase_Ncoord: 66..193
e-value: 2.9E-55
score: 186.0
IPR020810Enolase, C-terminal TIM barrel domainSMARTSM01192Enolase_C_3coord: 201..491
e-value: 6.9E-194
score: 660.1
IPR020810Enolase, C-terminal TIM barrel domainPFAMPF00113Enolase_Ccoord: 202..489
e-value: 5.6E-147
score: 488.7
IPR029017Enolase-like, N-terminalGENE3D3.30.390.10coord: 59..186
e-value: 2.6E-55
score: 188.1
IPR029017Enolase-like, N-terminalSUPERFAMILY54826Enolase N-terminal domain-likecoord: 65..196
IPR036849Enolase-like, C-terminal domain superfamilyGENE3D3.20.20.120coord: 187..491
e-value: 3.1E-152
score: 508.0
IPR036849Enolase-like, C-terminal domain superfamilySUPERFAMILY51604Enolase C-terminal domain-likecoord: 201..484
NoneNo IPR availableSFLDSFLDG00178enolasecoord: 66..478
e-value: 0.0
score: 623.6
NoneNo IPR availablePANTHERPTHR11902:SF42ENOLASE 1, CHLOROPLASTICcoord: 29..491
IPR020809Enolase, conserved sitePROSITEPS00164ENOLASEcoord: 401..414

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G03020.1CSPI01G03020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
biological_process GO:0010090 trichome morphogenesis
cellular_component GO:0009507 chloroplast
cellular_component GO:0000015 phosphopyruvate hydratase complex
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0004634 phosphopyruvate hydratase activity