CSPI01G03000 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G03000
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	protein CHAPERONE-LIKE PROTEIN OF POR1, chloroplastic
Location	Chr1: 1913973 .. 1916704 (+)
RNA-Seq Expression	CSPI01G03000
Synteny	CSPI01G03000

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACAAAACCCATCTTCTTGTTTCTTCTTTTTCCCTTTCTCCATTCGCCCAGATTTCGCAATATCCCTTTCTACCTTCTCGCTTCTATCTCCCATGGTGTTCGTCAAACCCTTAATTTAGGGTTTTCTTTTTTCTTCTATTTCCTCTGCGTCTTCTTTCTCTCCGAAGAAGAAGAAGAAGTTGAAAGAAGAATGGCTTCCGTTTCAATAACTAAACTTGATTTGGCCACCCCTTTCCTCGCTCAAAATCTGTAATTCCTCTCTCTCGTTTTCTCTAACCTTATTTATTGAGTTCTGTTGGGTTTGTCTTATTCATTTGGTGTTTTTTTTTTTTTTCTCTCTTTCCTAGGCCTGGTCGCCTTAACCGGTTGAATGGTAAACCATTTCGCGCGTTGTTTAGAAGCCCCAAGTGTGTTTTGGATACACCATATGAAGGTACTCTTCTATCTTCTTGCATTTCTATTCTTGAAACTGTTTCAATTGTGCATACTTAATTTCCAATTTTTTCATGGATTGGTTCTGTTGTTGTTTATGAAGTATGAAGGTATCAACAATTTCATATTGATTTTGATACTTTCTGCTCCAATCAATTTTTTTTTTTTGGATATTCTTAATATGGCATGATTGGAAGAACTAGGGATTATCCTTACCTCGAGAAACTAATGTGTCAAGGAACATTATCTGCTTTATTTCTGAAATTCTAATCTCGTTGGCTCTAAGTATAAATAAGAAAATGTGACATAGCTAGTGGATTATAGAACTTGTGTGATATGAAATTTGATATAGTTGCGTTAAGACTCAACCTACTAGTTGCGTTTTTGTAGTATTCTCTGACTCAAAGCTTGTTAAATTTGTTTGTCTAAGAAATGTCCATAATTTTTCCTAGCTGCTATGGTGCTAATCTGTCTATCCTTAATTATTCATAGGTAATGTCACAAAGTTCCCTCGAGTTCGTGTTTGGGATCCATATAAACGCCTTGGTGTAAGTTCTGATGCTTCAGAGGAAGAAATTTGGGGTGCACGGAATTTTCTTTTGCAGCAGTATTCTGGTCATGAAAGAAGTGAGGAATCTATAGAAGCTGCTTTTGAAAAAATATTGATGGCAAGCTTCCAGCATAGGAAAAAGACGAAGATTAATTTGAAAACCAGGTTGAAGAAGCAAGTAGAGGAGTCCCCACCTTGGATTAAGAACTTACTGAATTTTGTAGAACTTCCTCCAACTGATGTTATCTTCAGAAGATTGTTTCTTTTCGCATTTATGGGTGGCTGGAGTATTATGAACTCGGCCGAAGGTGGACCTGCTTTTCAGGTTAGTTCAATATTCTTCCAGTTAACATATGCTTTCCTTTCTAATGTAATCTCAGATTCTATGTATAAACATATTTTAGCGATATTCCAAAGGTTAGTGCTTTTGTTGGGAAGTAATCAAAGGGCTATAACATTGGAGGTACATGCTTTGAATTTTAGTTAGGCTGTTAAGAGTGGCAAAGGAAGAAGAAGAATACCTGGGAACTTGCCACAAAATGGATACCTCTACCTTGTCACATCAGCAATTCTTCTTTCAAATTTTAATTAAACTTAATAATAACAACCACAAGGAATTAATAAGATAAGTCGAGGTGTGTGTAAGCTAGCCTGAACACTCACACACACATATATAAAAGAAGATATGGAAAAATAGGGAAAGCAGAGAGTTCAGGGTTATAAGCCCATCTTCACCATTTGCTGTAGTAATGCCGTAAGTTATTTGCTTCTTCAATTCTCCTTTTTGTGACAGTGATCATGATTTAGTGGGTTGAATATTCAAATTCTGAATCTTGATTATCATCCAGGAAGAGTTAGATTATTGTGTTAGTTCTGCTATGTCCTGTAAGATTTAGTATTAAATTGAACACTTGAAATTAAGAATAGATGGGTGCACAGAAGTCTTGAAGACTATACGGATGTAAGATAAAGGTTGTGATCAAGGGAAGGGTGCCTATCTTGCTAATCTATTAGTCATTTTAACGAATCCATCCATTGCGGTCACTTTACTTCATGTGTTCATCTTAGCTTTCTTGCTCTTCCTCTAGTCAAGCCGTTTTCAAATTATGCAAACTTGTTCATTTAGGGCTGTCCCACCTAAAAACACATTGTCTTTTTTGTCAAAATTGGGTGCTGTTGAGATGCTTTAGGCCACCATATTCATTTCTGATTAAACTCTGTATATCAATCAATGCAGGTTGCAGTGTCCTTGGCGGCTTGCATATATTTCCTAAATGAGAAGACAAAAAGTGTGGCTCGAGCTTCCATTATAGGGTAAGACTTTGCACAAATTGCTCATCTCCTTATTTATCTCAAATAGAAAAATCAAAACCCTGTGGCTCATCTGCTCCCTCTTACTGTTCTACAGATTTGGTTCTCTTGTGGTCGGTTGGGTATGCGGTTCGTTGGTCGTTCCGAGTATTCCTTCTTTTCTATTGCAACCAACGTGGTCACTCGAACTTCTTACATCTCTAGTAGTATACTTCTTCTTGTTTCTTTCCTGTACTTTTCTCAAATAGATGTCTCTATGTTATTCTTAGCTTATGATCAAGAAGTTGTGATAAATGGATTTAATGGGGAAATTTACAGATATTATTGATCGGAGCCTATCTGTGAGCCTCCATTTTCTTCTTTGATTTCAATTATCGACTTAACTTTTGTTATCACCATTCTATTCCTTTACATCTCAAGATATAAGCCATTGTGGGTGC

mRNA sequence

ACAAAACCCATCTTCTTGTTTCTTCTTTTTCCCTTTCTCCATTCGCCCAGATTTCGCAATATCCCTTTCTACCTTCTCGCTTCTATCTCCCATGGTGTTCGTCAAACCCTTAATTTAGGGTTTTCTTTTTTCTTCTATTTCCTCTGCGTCTTCTTTCTCTCCGAAGAAGAAGAAGAAGTTGAAAGAAGAATGGCTTCCGTTTCAATAACTAAACTTGATTTGGCCACCCCTTTCCTCGCTCAAAATCTGCCTGGTCGCCTTAACCGGTTGAATGGTAAACCATTTCGCGCGTTGTTTAGAAGCCCCAAGTGTGTTTTGGATACACCATATGAAGGTAATGTCACAAAGTTCCCTCGAGTTCGTGTTTGGGATCCATATAAACGCCTTGGTGTAAGTTCTGATGCTTCAGAGGAAGAAATTTGGGGTGCACGGAATTTTCTTTTGCAGCAGTATTCTGGTCATGAAAGAAGTGAGGAATCTATAGAAGCTGCTTTTGAAAAAATATTGATGGCAAGCTTCCAGCATAGGAAAAAGACGAAGATTAATTTGAAAACCAGGTTGAAGAAGCAAGTAGAGGAGTCCCCACCTTGGATTAAGAACTTACTGAATTTTGTAGAACTTCCTCCAACTGATGTTATCTTCAGAAGATTGTTTCTTTTCGCATTTATGGGTGGCTGGAGTATTATGAACTCGGCCGAAGGTGGACCTGCTTTTCAGGTTGCAGTGTCCTTGGCGGCTTGCATATATTTCCTAAATGAGAAGACAAAAAGTGTGGCTCGAGCTTCCATTATAGGATTTGGTTCTCTTGTGGTCGGTTGGGTATGCGGTTCGTTGGTCGTTCCGAGTATTCCTTCTTTTCTATTGCAACCAACGTGGTCACTCGAACTTCTTACATCTCTAGTAGTATACTTCTTCTTGTTTCTTTCCTGTACTTTTCTCAAATAGATGTCTCTATGTTATTCTTAGCTTATGATCAAGAAGTTGTGATAAATGGATTTAATGGGGAAATTTACAGATATTATTGATCGGAGCCTATCTGTGAGCCTCCATTTTCTTCTTTGATTTCAATTATCGACTTAACTTTTGTTATCACCATTCTATTCCTTTACATCTCAAGATATAAGCCATTGTGGGTGC

Coding sequence (CDS)

ATGGCTTCCGTTTCAATAACTAAACTTGATTTGGCCACCCCTTTCCTCGCTCAAAATCTGCCTGGTCGCCTTAACCGGTTGAATGGTAAACCATTTCGCGCGTTGTTTAGAAGCCCCAAGTGTGTTTTGGATACACCATATGAAGGTAATGTCACAAAGTTCCCTCGAGTTCGTGTTTGGGATCCATATAAACGCCTTGGTGTAAGTTCTGATGCTTCAGAGGAAGAAATTTGGGGTGCACGGAATTTTCTTTTGCAGCAGTATTCTGGTCATGAAAGAAGTGAGGAATCTATAGAAGCTGCTTTTGAAAAAATATTGATGGCAAGCTTCCAGCATAGGAAAAAGACGAAGATTAATTTGAAAACCAGGTTGAAGAAGCAAGTAGAGGAGTCCCCACCTTGGATTAAGAACTTACTGAATTTTGTAGAACTTCCTCCAACTGATGTTATCTTCAGAAGATTGTTTCTTTTCGCATTTATGGGTGGCTGGAGTATTATGAACTCGGCCGAAGGTGGACCTGCTTTTCAGGTTGCAGTGTCCTTGGCGGCTTGCATATATTTCCTAAATGAGAAGACAAAAAGTGTGGCTCGAGCTTCCATTATAGGATTTGGTTCTCTTGTGGTCGGTTGGGTATGCGGTTCGTTGGTCGTTCCGAGTATTCCTTCTTTTCTATTGCAACCAACGTGGTCACTCGAACTTCTTACATCTCTAGTAGTATACTTCTTCTTGTTTCTTTCCTGTACTTTTCTCAAATAG

Protein sequence

MASVSITKLDLATPFLAQNLPGRLNRLNGKPFRALFRSPKCVLDTPYEGNVTKFPRVRVWDPYKRLGVSSDASEEEIWGARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINLKTRLKKQVEESPPWIKNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVSLAACIYFLNEKTKSVARASIIGFGSLVVGWVCGSLVVPSIPSFLLQPTWSLELLTSLVVYFFLFLSCTFLK*

Homology

[Close]

BLAST of CSPI01G03000 vs. ExPASy Swiss-Prot
Match: Q9FN50 (Protein CHAPERONE-LIKE PROTEIN OF POR1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CPP1 PE=1 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 2.9e-97
Identity = 176/256 (68.75%), Postives = 213/256 (83.20%), Query Frame = 0


Query: 2   ASVSITKLDLATPFLAQNLPGRL---NRLNGKPFRAL---FRSPKCVLDTPYEGNVTKFP 61
           +S+ ++   +++ F+A + P  +   ++ +  PFR +   F++ KC +D+ Y GNV  FP
Sbjct: 3   SSLLLSGSTVSSSFIAPSKPSLVRNSSKTSLLPFRNVSRSFKTVKCTVDSSYGGNVPTFP 62

Query: 62  RVRVWDPYKRLGVSSDASEEEIWGARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKK 121
           R RVWDPYKRLGVS  ASEEEIW +RNFLLQQY+GHERSEESIE AFEK+LM+SF  RKK
Sbjct: 63  RTRVWDPYKRLGVSPYASEEEIWASRNFLLQQYAGHERSEESIEGAFEKLLMSSFIRRKK 122

Query: 122 TKINLKTRLKKQVEESPPWIKNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAF 181
           TKINLK++LKK+VEESPPW+K LL+FVE+PP D IFRRLFLFAFMGGWSIMNSAEGGPAF
Sbjct: 123 TKINLKSKLKKKVEESPPWLKALLDFVEMPPMDTIFRRLFLFAFMGGWSIMNSAEGGPAF 182

Query: 182 QVAVSLAACIYFLNEKTKSVARASIIGFGSLVVGWVCGSLVVPSIPSFLLQPTWSLELLT 241
           QVAVSLAAC+YFLNEKTKS+ RA +IG G+LV GW CGSL++P IP+FL+QPTW+LELLT
Sbjct: 183 QVAVSLAACVYFLNEKTKSLGRACLIGIGALVAGWFCGSLIIPMIPTFLIQPTWTLELLT 242

Query: 242 SLVVYFFLFLSCTFLK 252
           SLV Y FLFLSCTFLK
Sbjct: 243 SLVAYVFLFLSCTFLK 258

[Close]

BLAST of CSPI01G03000 vs. ExPASy Swiss-Prot
Match: G5DBJ0 (Protein CHAPERONE-LIKE PROTEIN OF POR1, chloroplastic OS=Nicotiana benthamiana OX=4100 GN=CPP1 PE=1 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 3.5e-95
Identity = 171/252 (67.86%), Postives = 198/252 (78.57%), Query Frame = 0


Query: 1   MASVSITKLDLATPFLAQNLPGRLNRLNGKPFRALFRSPKC-VLDTPYEGNVTKFPRVRV 60
           MA+  I+KL L++ FL Q    R N +   P     R P+C   DTPY GN+ +FPRV V
Sbjct: 1   MATTLISKLTLSSAFLGQQFSSRGNSMRSAPAGLFLRGPRCAATDTPYGGNIPQFPRVNV 60

Query: 61  WDPYKRLGVSSDASEEEIWGARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKIN 120
           WDPYKRLG+S DASEEE+W +RNFLL QY  HERS ESIEAAFEKILMASF +RKKTKIN
Sbjct: 61  WDPYKRLGISRDASEEEVWSSRNFLLNQYYNHERSAESIEAAFEKILMASFINRKKTKIN 120

Query: 121 LKTRLKKQVEESPPWIKNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAV 180
           LKTRLKK+VEESPPW++NLL+FVELPP  +I RRLFLF FM  WS+MNS E GPAFQVA+
Sbjct: 121 LKTRLKKKVEESPPWVQNLLSFVELPPPVIILRRLFLFGFMACWSVMNSTEAGPAFQVAI 180

Query: 181 SLAACIYFLNEKTKSVARASIIGFGSLVVGWVCGSLVVPSIPSFLLQPTWSLELLTSLVV 240
           S  AC+YFLN+KTKS+ RA++IGFG+LV GW CGSL+VP IP  LL PTWSLELLTSL +
Sbjct: 181 SFGACVYFLNDKTKSLGRAALIGFGALVAGWFCGSLLVPMIPPNLLHPTWSLELLTSLFI 240

Query: 241 YFFLFLSCTFLK 252
           Y  LFL CTFLK
Sbjct: 241 YVSLFLGCTFLK 252

[Close]

BLAST of CSPI01G03000 vs. ExPASy TrEMBL
Match: A0A1S3BVF6 (protein CHAPERONE-LIKE PROTEIN OF POR1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103494090 PE=4 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.0e-134
Identity = 245/251 (97.61%), Postives = 248/251 (98.80%), Query Frame = 0


Query: 1   MASVSITKLDLATPFLAQNLPGRLNRLNGKPFRALFRSPKCVLDTPYEGNVTKFPRVRVW 60
           MASVSITKLDLATPFLAQNLPGRLNRLNGKPFRALFRSPKCVLDTPYEGNVTKFPR+RVW
Sbjct: 1   MASVSITKLDLATPFLAQNLPGRLNRLNGKPFRALFRSPKCVLDTPYEGNVTKFPRIRVW 60

Query: 61  DPYKRLGVSSDASEEEIWGARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINL 120
           DPYKRLGVSS ASEEEIW ARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINL
Sbjct: 61  DPYKRLGVSSAASEEEIWSARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINL 120

Query: 121 KTRLKKQVEESPPWIKNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVS 180
           KTRLKKQV+ESPPWIKNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVS
Sbjct: 121 KTRLKKQVDESPPWIKNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVS 180

Query: 181 LAACIYFLNEKTKSVARASIIGFGSLVVGWVCGSLVVPSIPSFLLQPTWSLELLTSLVVY 240
           LAACIYFLNEKTKS+ARASIIGFGSLVVGWVCGSLV PSIPSFLLQPTWSLELLTSLVVY
Sbjct: 181 LAACIYFLNEKTKSLARASIIGFGSLVVGWVCGSLVGPSIPSFLLQPTWSLELLTSLVVY 240

Query: 241 FFLFLSCTFLK 252
           FFLFLSCTFLK
Sbjct: 241 FFLFLSCTFLK 251

[Close]

BLAST of CSPI01G03000 vs. ExPASy TrEMBL
Match: A0A6J1EUP8 (protein CHAPERONE-LIKE PROTEIN OF POR1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111437768 PE=4 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 9.4e-128
Identity = 232/251 (92.43%), Postives = 238/251 (94.82%), Query Frame = 0


Query: 1   MASVSITKLDLATPFLAQNLPGRLNRLNGKPFRALFRSPKCVLDTPYEGNVTKFPRVRVW 60
           MAS S+T LDLATPFL QNL GRLNR  GKPF ALFRSPKCVLDTPYEGNVTKFPR+RVW
Sbjct: 1   MASFSLTNLDLATPFLGQNLSGRLNRAKGKPFCALFRSPKCVLDTPYEGNVTKFPRIRVW 60

Query: 61  DPYKRLGVSSDASEEEIWGARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINL 120
           DPYKRLG+S+ ASEEEIW ARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINL
Sbjct: 61  DPYKRLGISTAASEEEIWSARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINL 120

Query: 121 KTRLKKQVEESPPWIKNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVS 180
           KTRLKKQVEESPPW KNLLNFVELPPTDVIFRR+FLFAFMGGWSIMNSAEGGPAFQVAVS
Sbjct: 121 KTRLKKQVEESPPWFKNLLNFVELPPTDVIFRRMFLFAFMGGWSIMNSAEGGPAFQVAVS 180

Query: 181 LAACIYFLNEKTKSVARASIIGFGSLVVGWVCGSLVVPSIPSFLLQPTWSLELLTSLVVY 240
           LAACIYFLNEKTKS+ARASIIG GSLV GWVCGSLVVPSIPSFLLQPTWSLELLTSLVVY
Sbjct: 181 LAACIYFLNEKTKSLARASIIGLGSLVAGWVCGSLVVPSIPSFLLQPTWSLELLTSLVVY 240

Query: 241 FFLFLSCTFLK 252
            FLFLSCTFLK
Sbjct: 241 VFLFLSCTFLK 251

[Close]

BLAST of CSPI01G03000 vs. ExPASy TrEMBL
Match: A0A6J1L770 (protein CHAPERONE-LIKE PROTEIN OF POR1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111499791 PE=4 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 2.7e-127
Identity = 233/251 (92.83%), Postives = 237/251 (94.42%), Query Frame = 0


Query: 1   MASVSITKLDLATPFLAQNLPGRLNRLNGKPFRALFRSPKCVLDTPYEGNVTKFPRVRVW 60
           MAS S+T LDLAT FL QNL GRLNR  GKPF ALFRSPKCVLDTPYEGNVTKFPR+RVW
Sbjct: 1   MASFSLTNLDLATSFLGQNLSGRLNRAKGKPFCALFRSPKCVLDTPYEGNVTKFPRIRVW 60

Query: 61  DPYKRLGVSSDASEEEIWGARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINL 120
           DPYKRLGVS+ ASEEEIW ARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINL
Sbjct: 61  DPYKRLGVSTAASEEEIWSARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINL 120

Query: 121 KTRLKKQVEESPPWIKNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVS 180
           KTRLKKQVEESPPW KNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVS
Sbjct: 121 KTRLKKQVEESPPWFKNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVS 180

Query: 181 LAACIYFLNEKTKSVARASIIGFGSLVVGWVCGSLVVPSIPSFLLQPTWSLELLTSLVVY 240
           LAACIYFLNEKTKS+ARASIIG GSLV GWVCGSLVVPSIPSFLLQPTWSLELLTSLVVY
Sbjct: 181 LAACIYFLNEKTKSLARASIIGLGSLVAGWVCGSLVVPSIPSFLLQPTWSLELLTSLVVY 240

Query: 241 FFLFLSCTFLK 252
            FLFLSCTFLK
Sbjct: 241 IFLFLSCTFLK 251

[Close]

BLAST of CSPI01G03000 vs. ExPASy TrEMBL
Match: A0A0A0LUP3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G014380 PE=4 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 1.0e-126
Identity = 230/232 (99.14%), Postives = 232/232 (100.00%), Query Frame = 0


Query: 20  LPGRLNRLNGKPFRALFRSPKCVLDTPYEGNVTKFPRVRVWDPYKRLGVSSDASEEEIWG 79
           +PGRLNRLNGKPFRALFRSP+CVLDTPYEGNVTKFPRVRVWDPYKRLGVSSDASEEEIWG
Sbjct: 2   VPGRLNRLNGKPFRALFRSPRCVLDTPYEGNVTKFPRVRVWDPYKRLGVSSDASEEEIWG 61

Query: 80  ARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINLKTRLKKQVEESPPWIKNLL 139
           ARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINLKTRLKKQVEESPPWIKNLL
Sbjct: 62  ARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINLKTRLKKQVEESPPWIKNLL 121

Query: 140 NFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVSLAACIYFLNEKTKSVARAS 199
           NFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVSLAACIYFLNEKTKSVARAS
Sbjct: 122 NFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVSLAACIYFLNEKTKSVARAS 181

Query: 200 IIGFGSLVVGWVCGSLVVPSIPSFLLQPTWSLELLTSLVVYFFLFLSCTFLK 252
           IIGFGSLVVGWVCGSLVVPSIPSFLLQPTWSLELLTSLVVYFFLFLSCTFLK
Sbjct: 182 IIGFGSLVVGWVCGSLVVPSIPSFLLQPTWSLELLTSLVVYFFLFLSCTFLK 233

[Close]

BLAST of CSPI01G03000 vs. ExPASy TrEMBL
Match: A0A6J1JHA1 (protein CHAPERONE-LIKE PROTEIN OF POR1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111485707 PE=4 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 8.8e-126
Identity = 229/251 (91.24%), Postives = 237/251 (94.42%), Query Frame = 0


Query: 1   MASVSITKLDLATPFLAQNLPGRLNRLNGKPFRALFRSPKCVLDTPYEGNVTKFPRVRVW 60
           MASVS+T LDLATPFL  NL  RLN + G+PFRALFRSP+CVLDTPYEGNVTKF R+RVW
Sbjct: 1   MASVSLTNLDLATPFLGLNLSSRLNGVKGRPFRALFRSPRCVLDTPYEGNVTKFSRIRVW 60

Query: 61  DPYKRLGVSSDASEEEIWGARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINL 120
           DPYKRLGVS+ ASEEEIW ARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINL
Sbjct: 61  DPYKRLGVSTAASEEEIWSARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINL 120

Query: 121 KTRLKKQVEESPPWIKNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVS 180
           KTRLKKQVEESPPW KNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVS
Sbjct: 121 KTRLKKQVEESPPWFKNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVS 180

Query: 181 LAACIYFLNEKTKSVARASIIGFGSLVVGWVCGSLVVPSIPSFLLQPTWSLELLTSLVVY 240
           LAACIYFLNEKTK+VARASIIG GSLV GWVCGSL+VPSIPSFLLQPTWSLELLTSLVVY
Sbjct: 181 LAACIYFLNEKTKTVARASIIGLGSLVAGWVCGSLIVPSIPSFLLQPTWSLELLTSLVVY 240

Query: 241 FFLFLSCTFLK 252
            FLFLSCTFLK
Sbjct: 241 VFLFLSCTFLK 251

[Close]

BLAST of CSPI01G03000 vs. NCBI nr
Match: XP_004138201.1 (protein CHAPERONE-LIKE PROTEIN OF POR1, chloroplastic [Cucumis sativus] >KAE8652439.1 hypothetical protein Csa_014147 [Cucumis sativus])

HSP 1 Score: 498.8 bits (1283), Expect = 2.7e-137
Identity = 250/251 (99.60%), Postives = 251/251 (100.00%), Query Frame = 0


Query: 1   MASVSITKLDLATPFLAQNLPGRLNRLNGKPFRALFRSPKCVLDTPYEGNVTKFPRVRVW 60
           MASVSITKLDLATPFLAQNLPGRLNRLNGKPFRALFRSP+CVLDTPYEGNVTKFPRVRVW
Sbjct: 1   MASVSITKLDLATPFLAQNLPGRLNRLNGKPFRALFRSPRCVLDTPYEGNVTKFPRVRVW 60

Query: 61  DPYKRLGVSSDASEEEIWGARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINL 120
           DPYKRLGVSSDASEEEIWGARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINL
Sbjct: 61  DPYKRLGVSSDASEEEIWGARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINL 120

Query: 121 KTRLKKQVEESPPWIKNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVS 180
           KTRLKKQVEESPPWIKNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVS
Sbjct: 121 KTRLKKQVEESPPWIKNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVS 180

Query: 181 LAACIYFLNEKTKSVARASIIGFGSLVVGWVCGSLVVPSIPSFLLQPTWSLELLTSLVVY 240
           LAACIYFLNEKTKSVARASIIGFGSLVVGWVCGSLVVPSIPSFLLQPTWSLELLTSLVVY
Sbjct: 181 LAACIYFLNEKTKSVARASIIGFGSLVVGWVCGSLVVPSIPSFLLQPTWSLELLTSLVVY 240

Query: 241 FFLFLSCTFLK 252
           FFLFLSCTFLK
Sbjct: 241 FFLFLSCTFLK 251

[Close]

BLAST of CSPI01G03000 vs. NCBI nr
Match: XP_008453340.1 (PREDICTED: protein CHAPERONE-LIKE PROTEIN OF POR1, chloroplastic [Cucumis melo])

HSP 1 Score: 489.2 bits (1258), Expect = 2.1e-134
Identity = 245/251 (97.61%), Postives = 248/251 (98.80%), Query Frame = 0


Query: 1   MASVSITKLDLATPFLAQNLPGRLNRLNGKPFRALFRSPKCVLDTPYEGNVTKFPRVRVW 60
           MASVSITKLDLATPFLAQNLPGRLNRLNGKPFRALFRSPKCVLDTPYEGNVTKFPR+RVW
Sbjct: 1   MASVSITKLDLATPFLAQNLPGRLNRLNGKPFRALFRSPKCVLDTPYEGNVTKFPRIRVW 60

Query: 61  DPYKRLGVSSDASEEEIWGARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINL 120
           DPYKRLGVSS ASEEEIW ARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINL
Sbjct: 61  DPYKRLGVSSAASEEEIWSARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINL 120

Query: 121 KTRLKKQVEESPPWIKNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVS 180
           KTRLKKQV+ESPPWIKNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVS
Sbjct: 121 KTRLKKQVDESPPWIKNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVS 180

Query: 181 LAACIYFLNEKTKSVARASIIGFGSLVVGWVCGSLVVPSIPSFLLQPTWSLELLTSLVVY 240
           LAACIYFLNEKTKS+ARASIIGFGSLVVGWVCGSLV PSIPSFLLQPTWSLELLTSLVVY
Sbjct: 181 LAACIYFLNEKTKSLARASIIGFGSLVVGWVCGSLVGPSIPSFLLQPTWSLELLTSLVVY 240

Query: 241 FFLFLSCTFLK 252
           FFLFLSCTFLK
Sbjct: 241 FFLFLSCTFLK 251

[Close]

BLAST of CSPI01G03000 vs. NCBI nr
Match: XP_038878491.1 (protein CHAPERONE-LIKE PROTEIN OF POR1, chloroplastic [Benincasa hispida])

HSP 1 Score: 470.3 bits (1209), Expect = 1.0e-128
Identity = 233/251 (92.83%), Postives = 240/251 (95.62%), Query Frame = 0


Query: 1   MASVSITKLDLATPFLAQNLPGRLNRLNGKPFRALFRSPKCVLDTPYEGNVTKFPRVRVW 60
           MASVS+T LDL TPFL QNLPGRLNRLNGKPFR LFRSP+CVLDTPYEGNVTKFPR RVW
Sbjct: 1   MASVSLTNLDLTTPFLGQNLPGRLNRLNGKPFRVLFRSPRCVLDTPYEGNVTKFPRTRVW 60

Query: 61  DPYKRLGVSSDASEEEIWGARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINL 120
           DPYKRLGVS+ ASEEEIW ARNFLLQQYSGHERSEE+IEAAFEKILMASFQ RKKTKINL
Sbjct: 61  DPYKRLGVSTAASEEEIWSARNFLLQQYSGHERSEEAIEAAFEKILMASFQRRKKTKINL 120

Query: 121 KTRLKKQVEESPPWIKNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVS 180
           KTRLKKQVEESPPWIKNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVS
Sbjct: 121 KTRLKKQVEESPPWIKNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVS 180

Query: 181 LAACIYFLNEKTKSVARASIIGFGSLVVGWVCGSLVVPSIPSFLLQPTWSLELLTSLVVY 240
           LAACIYFLNEKTKS+ARASIIG GSLV GW+CGSLVVP+IPSFLLQPTWSLELLTSLVVY
Sbjct: 181 LAACIYFLNEKTKSLARASIIGLGSLVAGWICGSLVVPTIPSFLLQPTWSLELLTSLVVY 240

Query: 241 FFLFLSCTFLK 252
            FLFLSCTFLK
Sbjct: 241 IFLFLSCTFLK 251

[Close]

BLAST of CSPI01G03000 vs. NCBI nr
Match: XP_022931604.1 (protein CHAPERONE-LIKE PROTEIN OF POR1, chloroplastic [Cucurbita moschata] >KAG7021598.1 Protein CHAPERONE-LIKE PROTEIN OF POR1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 466.1 bits (1198), Expect = 1.9e-127
Identity = 232/251 (92.43%), Postives = 238/251 (94.82%), Query Frame = 0


Query: 1   MASVSITKLDLATPFLAQNLPGRLNRLNGKPFRALFRSPKCVLDTPYEGNVTKFPRVRVW 60
           MAS S+T LDLATPFL QNL GRLNR  GKPF ALFRSPKCVLDTPYEGNVTKFPR+RVW
Sbjct: 1   MASFSLTNLDLATPFLGQNLSGRLNRAKGKPFCALFRSPKCVLDTPYEGNVTKFPRIRVW 60

Query: 61  DPYKRLGVSSDASEEEIWGARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINL 120
           DPYKRLG+S+ ASEEEIW ARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINL
Sbjct: 61  DPYKRLGISTAASEEEIWSARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINL 120

Query: 121 KTRLKKQVEESPPWIKNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVS 180
           KTRLKKQVEESPPW KNLLNFVELPPTDVIFRR+FLFAFMGGWSIMNSAEGGPAFQVAVS
Sbjct: 121 KTRLKKQVEESPPWFKNLLNFVELPPTDVIFRRMFLFAFMGGWSIMNSAEGGPAFQVAVS 180

Query: 181 LAACIYFLNEKTKSVARASIIGFGSLVVGWVCGSLVVPSIPSFLLQPTWSLELLTSLVVY 240
           LAACIYFLNEKTKS+ARASIIG GSLV GWVCGSLVVPSIPSFLLQPTWSLELLTSLVVY
Sbjct: 181 LAACIYFLNEKTKSLARASIIGLGSLVAGWVCGSLVVPSIPSFLLQPTWSLELLTSLVVY 240

Query: 241 FFLFLSCTFLK 252
            FLFLSCTFLK
Sbjct: 241 VFLFLSCTFLK 251

[Close]

BLAST of CSPI01G03000 vs. NCBI nr
Match: XP_023007253.1 (protein CHAPERONE-LIKE PROTEIN OF POR1, chloroplastic [Cucurbita maxima])

HSP 1 Score: 464.5 bits (1194), Expect = 5.7e-127
Identity = 233/251 (92.83%), Postives = 237/251 (94.42%), Query Frame = 0


Query: 1   MASVSITKLDLATPFLAQNLPGRLNRLNGKPFRALFRSPKCVLDTPYEGNVTKFPRVRVW 60
           MAS S+T LDLAT FL QNL GRLNR  GKPF ALFRSPKCVLDTPYEGNVTKFPR+RVW
Sbjct: 1   MASFSLTNLDLATSFLGQNLSGRLNRAKGKPFCALFRSPKCVLDTPYEGNVTKFPRIRVW 60

Query: 61  DPYKRLGVSSDASEEEIWGARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINL 120
           DPYKRLGVS+ ASEEEIW ARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINL
Sbjct: 61  DPYKRLGVSTAASEEEIWSARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINL 120

Query: 121 KTRLKKQVEESPPWIKNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVS 180
           KTRLKKQVEESPPW KNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVS
Sbjct: 121 KTRLKKQVEESPPWFKNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAFQVAVS 180

Query: 181 LAACIYFLNEKTKSVARASIIGFGSLVVGWVCGSLVVPSIPSFLLQPTWSLELLTSLVVY 240
           LAACIYFLNEKTKS+ARASIIG GSLV GWVCGSLVVPSIPSFLLQPTWSLELLTSLVVY
Sbjct: 181 LAACIYFLNEKTKSLARASIIGLGSLVAGWVCGSLVVPSIPSFLLQPTWSLELLTSLVVY 240

Query: 241 FFLFLSCTFLK 252
            FLFLSCTFLK
Sbjct: 241 IFLFLSCTFLK 251

[Close]

BLAST of CSPI01G03000 vs. TAIR 10
Match: AT5G23040.1 (Protein of unknown function (DUF3353) )

HSP 1 Score: 356.3 bits (913), Expect = 2.0e-98
Identity = 176/256 (68.75%), Postives = 213/256 (83.20%), Query Frame = 0


Query: 2   ASVSITKLDLATPFLAQNLPGRL---NRLNGKPFRAL---FRSPKCVLDTPYEGNVTKFP 61
           +S+ ++   +++ F+A + P  +   ++ +  PFR +   F++ KC +D+ Y GNV  FP
Sbjct: 3   SSLLLSGSTVSSSFIAPSKPSLVRNSSKTSLLPFRNVSRSFKTVKCTVDSSYGGNVPTFP 62

Query: 62  RVRVWDPYKRLGVSSDASEEEIWGARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKK 121
           R RVWDPYKRLGVS  ASEEEIW +RNFLLQQY+GHERSEESIE AFEK+LM+SF  RKK
Sbjct: 63  RTRVWDPYKRLGVSPYASEEEIWASRNFLLQQYAGHERSEESIEGAFEKLLMSSFIRRKK 122

Query: 122 TKINLKTRLKKQVEESPPWIKNLLNFVELPPTDVIFRRLFLFAFMGGWSIMNSAEGGPAF 181
           TKINLK++LKK+VEESPPW+K LL+FVE+PP D IFRRLFLFAFMGGWSIMNSAEGGPAF
Sbjct: 123 TKINLKSKLKKKVEESPPWLKALLDFVEMPPMDTIFRRLFLFAFMGGWSIMNSAEGGPAF 182

Query: 182 QVAVSLAACIYFLNEKTKSVARASIIGFGSLVVGWVCGSLVVPSIPSFLLQPTWSLELLT 241
           QVAVSLAAC+YFLNEKTKS+ RA +IG G+LV GW CGSL++P IP+FL+QPTW+LELLT
Sbjct: 183 QVAVSLAACVYFLNEKTKSLGRACLIGIGALVAGWFCGSLIIPMIPTFLIQPTWTLELLT 242

Query: 242 SLVVYFFLFLSCTFLK 252
           SLV Y FLFLSCTFLK
Sbjct: 243 SLVAYVFLFLSCTFLK 258

[Close]

BLAST of CSPI01G03000 vs. TAIR 10
Match: AT3G51140.1 (Protein of unknown function (DUF3353) )

HSP 1 Score: 135.2 bits (339), Expect = 7.3e-32
Identity = 78/203 (38.42%), Postives = 127/203 (62.56%), Query Frame = 0


Query: 54  FPRVRVWDPYKRLGVSSDASEEEIWGARNFLLQQYSGHERSEESIEAAFEKILMASFQHR 113
           FPR+ V DPYKRLG+S  ASE+EI GARNFL+QQY+GH+ S ++IE+A +KI+M  F  R
Sbjct: 83  FPRINVKDPYKRLGISRMASEDEIQGARNFLIQQYAGHKPSVDAIESAHDKIIMQKFHER 142

Query: 114 KKTKINLKTRLKKQVEESPPWIKNLLNFV----ELPPTDVIFRRLFLFAFMGGWSIMNSA 173
           K  KI++  ++ +QV +S      ++NFV    + PP  V+ +    FA +G  +++   
Sbjct: 143 KNPKIDISKKV-RQVRQS-----KVVNFVFERFQTPPNAVLVKTAVTFAVLGVLTVLFPT 202

Query: 174 EGGPAFQVAVSLAACIYFLNEKTKSVARASIIGFGSLVVGWVCGS-LVVPSIPSFLLQPT 233
           E GP  QV +SL A  YF++++ +      + G G+ +  W+ G+ L+V  IP F+  P 
Sbjct: 203 EEGPTLQVLLSLIATFYFIHQRLQKKLWTFLYGAGAFIFSWLVGTFLMVSVIPPFIKGPR 262

Query: 234 WSLELLTSLVVYFFLFLSCTFLK 252
              E+++SL+ Y  L+++ ++L+
Sbjct: 263 -GFEVMSSLLSYVLLWVASSYLR 278

[Close]

BLAST of CSPI01G03000 vs. TAIR 10
Match: AT2G20920.1 (Protein of unknown function (DUF3353) )

HSP 1 Score: 55.1 bits (131), Expect = 9.7e-08
Identity = 46/172 (26.74%), Postives = 75/172 (43.60%), Query Frame = 0


Query: 64  KRLGVSSDASEEEIWGARNFLLQQYSGHERSEESIEAAFEKILMASFQHRKKTKINLKTR 123
           K LGVS  AS +EI  A+  +L        +    EAA++ +LM S   R+  K+     
Sbjct: 79  KVLGVSEGASFDEILRAKKSILASRKDDPNAISQAEAAYDMLLMQSLNQRRAGKVVSNNI 138

Query: 124 LKKQVEESPP--------WIKNLLNFVELPPTD------VIFRRLFLFAFMGGWSIMNS- 183
               V+ S P        W+KN    V++P T        ++  + +  ++ G S  +S 
Sbjct: 139 RYADVKSSNPLGTSTVTQWMKNPPVSVDMPSTSDLGIQAGVYGAMMVLTYVNGSSFESSG 198

Query: 184 ----AEGGPAFQVAVSLAACIYFLNEKTKSVARASIIGFGSLVVGWVCGSLV 217
                   P   +A S  A +YF+ +K   + +A+ +  G LV G V GS +
Sbjct: 199 MPYAGADVPGLILASSFGASLYFMTKKKVKLGKAAALTAGGLVAGAVVGSAI 250

The following BLAST results are available for this feature:

BLAST of CSPI01G03000 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FN50	2.9e-97	68.75	Protein CHAPERONE-LIKE PROTEIN OF POR1, chloroplastic OS=Arabidopsis thaliana OX...	[more]
G5DBJ0	3.5e-95	67.86	Protein CHAPERONE-LIKE PROTEIN OF POR1, chloroplastic OS=Nicotiana benthamiana O...	[more]

BLAST of CSPI01G03000 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BVF6	1.0e-134	97.61	protein CHAPERONE-LIKE PROTEIN OF POR1, chloroplastic OS=Cucumis melo OX=3656 GN...	[more]
A0A6J1EUP8	9.4e-128	92.43	protein CHAPERONE-LIKE PROTEIN OF POR1, chloroplastic OS=Cucurbita moschata OX=3...	[more]
A0A6J1L770	2.7e-127	92.83	protein CHAPERONE-LIKE PROTEIN OF POR1, chloroplastic OS=Cucurbita maxima OX=366...	[more]
A0A0A0LUP3	1.0e-126	99.14	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G014380 PE=4 SV=1	[more]
A0A6J1JHA1	8.8e-126	91.24	protein CHAPERONE-LIKE PROTEIN OF POR1, chloroplastic-like OS=Cucurbita maxima O...	[more]

BLAST of CSPI01G03000 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004138201.1	2.7e-137	99.60	protein CHAPERONE-LIKE PROTEIN OF POR1, chloroplastic [Cucumis sativus] >KAE8652...	[more]
XP_008453340.1	2.1e-134	97.61	PREDICTED: protein CHAPERONE-LIKE PROTEIN OF POR1, chloroplastic [Cucumis melo]	[more]
XP_038878491.1	1.0e-128	92.83	protein CHAPERONE-LIKE PROTEIN OF POR1, chloroplastic [Benincasa hispida]	[more]
XP_022931604.1	1.9e-127	92.43	protein CHAPERONE-LIKE PROTEIN OF POR1, chloroplastic [Cucurbita moschata] >KAG7...	[more]
XP_023007253.1	5.7e-127	92.83	protein CHAPERONE-LIKE PROTEIN OF POR1, chloroplastic [Cucurbita maxima]	[more]

BLAST of CSPI01G03000 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G23040.1	2.0e-98	68.75	Protein of unknown function (DUF3353)	[more]
AT3G51140.1	7.3e-32	38.42	Protein of unknown function (DUF3353)	[more]
AT2G20920.1	9.7e-08	26.74	Protein of unknown function (DUF3353)	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021788	Protein CHAPERONE-LIKE PROTEIN OF POR1-like	PFAM	PF11833	CPP1-like	coord: 71..250 e-value: 5.5E-58 score: 195.9
IPR021788	Protein CHAPERONE-LIKE PROTEIN OF POR1-like	PANTHER	PTHR33372	FAMILY NOT NAMED	coord: 1..251
None	No IPR available	PANTHER	PTHR33372:SF2	PROTEIN CHAPERONE-LIKE PROTEIN OF POR1, CHLOROPLASTIC	coord: 1..251

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G03000.1	CSPI01G03000.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0031969	chloroplast membrane
cellular_component	GO:0016021	integral component of membrane