CSPI01G02550 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G02550
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	HNH domain-containing protein
Location	Chr1: 1618309 .. 1620671 (-)
RNA-Seq Expression	CSPI01G02550
Synteny	CSPI01G02550

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCCTTCTCCAATTTCCCCGTTCGGCCCATTCGGATTGTGGGCGGTGAGTCGGCCCGCTGAAAGAAGAGTCGAGGGGCAATGATCGGCGTAATTTAAGAAGAACGGAATGATCCAATGTCTTTAGTTTCTTCCATTTTTGTCTGCTGGACGGCGTCCCGACTCCGATGGAAGCTACTTCCAGTTCGCCGTCACCGACTCGTTCCCGCGGCGGGCAAAGAGATAGTGGCTCCGGAAGTGGGGACGAACGGCCAAGATTTTTCGACGCAAAAGCGAAAGCTAGTTGTTGGGCTAAGGCGGATGTTGTCCCTGGGCGGCATCCCGAGCGTTGGCGTAAGGACGCCGCTGGTAATGTCGTCTGTAAGCGCTTCTGTAACTGCCAGGGCTGCCTCTGTTTTGAGTATGATCACATTGTCCCTTACTCTAAAGGTAGTATGATTCTTTAGTCGACGATTGGTTTCTGATTCCGATTTGAGAAGTTTTGCTTCTGCTGTTGCGTTCAGGGTTTAGTTTGGATTGTGATGAAATTTGTCGGCGACTGTTTGCAGGAGGCGAATCCACGGCGGATAATTGCCAGATTCTTCAGACGAGAGTAAACAGATTCAAATCGAATAAAGACGATGTTGATACGTCTGAGTTGAAAAGCTATTCGTGTGATGTCAAGTTTACTGGTACTTCTTCATCCTGCTCCAATTCTTTCTTTTGGTATTCATATTCTTCTATTTTTCATATCCATTTATTGGCTGGTAATCTATGAGCTGCCGATACCAAGTTACATAAAATCAGATTTACATATATCTCACTTTCTAGTTTCCGTACTGCCTATAATTTTTCGCAAATTCTTTCTGCCGTACTATTCAATGCAAGAACTTCTCAAATTCATGCTTGTTTAAGCTACGAGTAAACCATCCGTTTCGAATACCAAAGTAGTTCACCCTACTGTTGTTTTGTTATTGCAATTTCTCCCAGTAAAATATAACTTTTGGCATTGATCTCTTTTTCTTAGCTGCCCCATATGGCCAAATGTTCATCAGCTTTTACGTAATCATATGGTTTGTTGCTCAAAATCTGTAGTTTCTGTAAAGTTGCTCAGCCATACCGTGAGACAATTGATTGGCTGTTGTGCTTTTATTTAACTTTTGTTGGTACTTGATGACAATTTCGGGGAAAGTGATCTAGAAACATCATTACAGTTTCAGGAAAGTGCTAGAAAGTGAAGTTTCAATTGTTTTAGAAAGATCTGTTGTCCTGTCCCATATTAGCTGTCATTTCTGGTGGTAGTTTCTGATTAAAAATTTAAAACAAACGAGTTCTTGTATTTTAGGTCATCTGAAACTTTAAGTGATGAATCTCCATAGGCTGCTGTCTAGAACTTCGAAGTATAAACTCACCTCCTTAAGGTTATGAATTCTAATATCTTATGAAAATTTCATTGAATGATGTAGTATAATTAAATTGCTGTGCATAAGCAGACAAGAGCACTCTCTGTTTATAAGATCGTCACCTCTTAGTTCATTGTGCTCCCTTTGAAACCTGCTTTCATGCATCTCAAGAAAGTCACTTTGTTACCAACCCACCAAAGAAACTCCTTTGACATTTTTTTTTTTGTATAGAAACTATTTTCTCATGTAAGGGCGTACAAAAAAGAAAAAAAAACAAGCCTGCACCTACTGAATAGAATGTGCATAAAGAGTAATTACAAAATGTGTTTGAAATGGAAGGCTAAACATCAATTGGATATCTCTCCTCCACCTCTCCCTAACCACAGTTATTTCTCTACCTCATCAATCTCACATTTAATCGCACACCCAACATCTCAAAGCAAGCAATCATTCTCACCAAAAGACTGATGGAGAACTCGCTCCACTATTGAACCACTAACGCTTGACCATCTCATGGTAGTCTATATTATAGAATCTGATTAAAACAGTTGATGCGTCAAGGTTTTTTCGCGTCTATCTCACATCCATATATGATGTATTTGGGCTTCATAATTCATTGCAGATAAGGAGCTTGACATAATTGAAATGGCTGTTTACGGAGATGTAATCCGGCCAGGGAACCAATGTCGTTGTAGAACAGTTTCTGAAGTGCTTGGGCAATATAAATCTAAAGACCGTTTGGCCCCCTGCAAATTGCCATATAATGAAGAAGGTTCTTTGTAACAACAAAGAAGACTTGAAACTAAAGCATGTTTGCCCCATCTATAGTTTAATACGATTGATAAACATCATTTTAAGATTACATCAATTAGAATTGTGTGGACCTCCATTACTTGATTAGATCAATATGAATCAACATCGACCATCATTTTTTTTTTATTATTAGGGTTTGCTTTCAAATATACTAAAATATTTATGCC

mRNA sequence

ATGGGCCTTCTCCAATTTCCCCGTTCGGCCCATTCGGATTGTGGGCGTTTCTTCCATTTTTGTCTGCTGGACGGCGTCCCGACTCCGATGGAAGCTACTTCCAGTTCGCCGTCACCGACTCGTTCCCGCGGCGGGCAAAGAGATAGTGGCTCCGGAAGTGGGGACGAACGGCCAAGATTTTTCGACGCAAAAGCGAAAGCTAGTTGTTGGGCTAAGGCGGATGTTGTCCCTGGGCGGCATCCCGAGCGTTGGCGTAAGGACGCCGCTGGTAATGTCGTCTGTAAGCGCTTCTGTAACTGCCAGGGCTGCCTCTGTTTTGAGTATGATCACATTGTCCCTTACTCTAAAGGAGGCGAATCCACGGCGGATAATTGCCAGATTCTTCAGACGAGAGTAAACAGATTCAAATCGAATAAAGACGATGTTGATACGTCTGAGTTGAAAAGCTATTCGTGTGATGTCAAGTTTACTGATAAGGAGCTTGACATAATTGAAATGGCTGTTTACGGAGATGTAATCCGGCCAGGGAACCAATGTCGTTGTAGAACAGTTTCTGAAGTGCTTGGGCAATATAAATCTAAAGACCGTTTGGCCCCCTGCAAATTGCCATATAATGAAGAAGGTTCTTTGTAACAACAAAGAAGACTTGAAACTAAAGCATGTTTGCCCCATCTATAGTTTAATACGATTGATAAACATCATTTTAAGATTACATCAATTAGAATTGTGTGGACCTCCATTACTTGATTAGATCAATATGAATCAACATCGACCATCATTTTTTTTTTATTATTAGGGTTTGCTTTCAAATATACTAAAATATTTATGCC

Coding sequence (CDS)

ATGGGCCTTCTCCAATTTCCCCGTTCGGCCCATTCGGATTGTGGGCGTTTCTTCCATTTTTGTCTGCTGGACGGCGTCCCGACTCCGATGGAAGCTACTTCCAGTTCGCCGTCACCGACTCGTTCCCGCGGCGGGCAAAGAGATAGTGGCTCCGGAAGTGGGGACGAACGGCCAAGATTTTTCGACGCAAAAGCGAAAGCTAGTTGTTGGGCTAAGGCGGATGTTGTCCCTGGGCGGCATCCCGAGCGTTGGCGTAAGGACGCCGCTGGTAATGTCGTCTGTAAGCGCTTCTGTAACTGCCAGGGCTGCCTCTGTTTTGAGTATGATCACATTGTCCCTTACTCTAAAGGAGGCGAATCCACGGCGGATAATTGCCAGATTCTTCAGACGAGAGTAAACAGATTCAAATCGAATAAAGACGATGTTGATACGTCTGAGTTGAAAAGCTATTCGTGTGATGTCAAGTTTACTGATAAGGAGCTTGACATAATTGAAATGGCTGTTTACGGAGATGTAATCCGGCCAGGGAACCAATGTCGTTGTAGAACAGTTTCTGAAGTGCTTGGGCAATATAAATCTAAAGACCGTTTGGCCCCCTGCAAATTGCCATATAATGAAGAAGGTTCTTTGTAA

Protein sequence

MGLLQFPRSAHSDCGRFFHFCLLDGVPTPMEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAAGNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKSYSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLPYNEEGSL*

Homology

[Close]

BLAST of CSPI01G02550 vs. ExPASy TrEMBL
Match: A0A0A0LUK3 (HNHc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G011480 PE=4 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 5.0e-122
Identity = 209/210 (99.52%), Postives = 209/210 (99.52%), Query Frame = 0


Query: 1   MGLLQFPRSAHSDCGRFFHFCLLDGVPTPMEATSSSPSPTRSRGGQRDSGSGSGDERPRF 60
           MGLLQFPRSAHSDCGRFFHFCLLDGVPTPMEATSSSPSPTRSRGGQRDSGSGSGDERPRF
Sbjct: 1   MGLLQFPRSAHSDCGRFFHFCLLDGVPTPMEATSSSPSPTRSRGGQRDSGSGSGDERPRF 60

Query: 61  FDAKAKASCWAKADVVPGRHPERWRKDAAGNVVCKRFCNCQGCLCFEYDHIVPYSKGGES 120
           FDAKAKASCWAKADVVPGRHPERWRKDAAGNVVCKRFCNCQGCLCFEYDHIVPYSKGGES
Sbjct: 61  FDAKAKASCWAKADVVPGRHPERWRKDAAGNVVCKRFCNCQGCLCFEYDHIVPYSKGGES 120

Query: 121 TADNCQILQTRVNRFKSNKDDVDTSELKSYSCDVKFTDKELDIIEMAVYGDVIRPGNQCR 180
           TADNCQILQTRVNRFKSNKDDVDTSELK YSCDVKFTDKELDIIEMAVYGDVIRPGNQCR
Sbjct: 121 TADNCQILQTRVNRFKSNKDDVDTSELKGYSCDVKFTDKELDIIEMAVYGDVIRPGNQCR 180

Query: 181 CRTVSEVLGQYKSKDRLAPCKLPYNEEGSL 211
           CRTVSEVLGQYKSKDRLAPCKLPYNEEGSL
Sbjct: 181 CRTVSEVLGQYKSKDRLAPCKLPYNEEGSL 210

[Close]

BLAST of CSPI01G02550 vs. ExPASy TrEMBL
Match: A0A5A7UWI1 (HNH endonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002690 PE=4 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 7.0e-100
Identity = 175/178 (98.31%), Postives = 177/178 (99.44%), Query Frame = 0


Query: 30  MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 89
           MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA
Sbjct: 1   MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 60

Query: 90  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKS 149
           GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELK 
Sbjct: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKG 120

Query: 150 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLPYNEE 208
           YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSE+LGQYKSKDRLAPCKLPYNE+
Sbjct: 121 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYNED 178

[Close]

BLAST of CSPI01G02550 vs. ExPASy TrEMBL
Match: A0A1S3BVP6 (uncharacterized protein LOC103494011 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494011 PE=4 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 7.0e-100
Identity = 175/178 (98.31%), Postives = 177/178 (99.44%), Query Frame = 0


Query: 30  MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 89
           MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA
Sbjct: 1   MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 60

Query: 90  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKS 149
           GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELK 
Sbjct: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKG 120

Query: 150 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLPYNEE 208
           YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSE+LGQYKSKDRLAPCKLPYNE+
Sbjct: 121 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYNED 178

[Close]

BLAST of CSPI01G02550 vs. ExPASy TrEMBL
Match: A0A6J1I7G1 (uncharacterized protein LOC111471509 OS=Cucurbita maxima OX=3661 GN=LOC111471509 PE=4 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 2.9e-98
Identity = 171/186 (91.94%), Postives = 178/186 (95.70%), Query Frame = 0


Query: 24  DGVPTPMEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPER 83
           DGV TPMEATSSSPSPTRSRGG R SGSG+G+ERPRFFD KAKA CWAKAD VPGRHPER
Sbjct: 6   DGVETPMEATSSSPSPTRSRGGPRQSGSGNGNERPRFFDTKAKAICWAKADTVPGRHPER 65

Query: 84  WRKDAAGNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVD 143
           WRKDAAGNVVCKRFCNCQGCLCFEYDHIVP+SKGGESTADNCQILQTRVNRFKS+KDDVD
Sbjct: 66  WRKDAAGNVVCKRFCNCQGCLCFEYDHIVPFSKGGESTADNCQILQTRVNRFKSDKDDVD 125

Query: 144 TSELKSYSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLP 203
           TS+LK YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSE+LGQYKSKDRLAPCKLP
Sbjct: 126 TSQLKGYSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLP 185

Query: 204 YNEEGS 210
           YNE+ S
Sbjct: 186 YNEDSS 191

[Close]

BLAST of CSPI01G02550 vs. ExPASy TrEMBL
Match: A0A6J1EZW3 (uncharacterized protein LOC111441065 OS=Cucurbita moschata OX=3662 GN=LOC111441065 PE=4 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 2.9e-98
Identity = 171/186 (91.94%), Postives = 178/186 (95.70%), Query Frame = 0


Query: 24  DGVPTPMEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPER 83
           DGV TPMEATSSSPSPTRSRGG R SGSG+G+ERPRFFD KAKA CWAKAD VPGRHPER
Sbjct: 6   DGVETPMEATSSSPSPTRSRGGPRQSGSGNGNERPRFFDTKAKAICWAKADTVPGRHPER 65

Query: 84  WRKDAAGNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVD 143
           WRKDAAGNVVCKRFCNCQGCLCFEYDHIVP+SKGGESTADNCQILQTRVNRFKS+KDDVD
Sbjct: 66  WRKDAAGNVVCKRFCNCQGCLCFEYDHIVPFSKGGESTADNCQILQTRVNRFKSDKDDVD 125

Query: 144 TSELKSYSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLP 203
           TS+LK YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSE+LGQYKSKDRLAPCKLP
Sbjct: 126 TSQLKGYSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLP 185

Query: 204 YNEEGS 210
           YNE+ S
Sbjct: 186 YNEDSS 191

[Close]

BLAST of CSPI01G02550 vs. NCBI nr
Match: XP_004138181.1 (uncharacterized protein LOC101204599 [Cucumis sativus] >KAE8652425.1 hypothetical protein Csa_014117 [Cucumis sativus])

HSP 1 Score: 381.3 bits (978), Expect = 5.3e-102
Identity = 180/181 (99.45%), Postives = 180/181 (99.45%), Query Frame = 0


Query: 30  MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 89
           MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA
Sbjct: 1   MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 60

Query: 90  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKS 149
           GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELK 
Sbjct: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKG 120

Query: 150 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLPYNEEGS 209
           YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLPYNEEGS
Sbjct: 121 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLPYNEEGS 180

Query: 210 L 211
           L
Sbjct: 181 L 181

[Close]

BLAST of CSPI01G02550 vs. NCBI nr
Match: XP_008453224.1 (PREDICTED: uncharacterized protein LOC103494011 isoform X2 [Cucumis melo] >KAA0057965.1 HNH endonuclease [Cucumis melo var. makuwa])

HSP 1 Score: 373.2 bits (957), Expect = 1.4e-99
Identity = 175/178 (98.31%), Postives = 177/178 (99.44%), Query Frame = 0


Query: 30  MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 89
           MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA
Sbjct: 1   MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 60

Query: 90  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKS 149
           GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELK 
Sbjct: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKG 120

Query: 150 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLPYNEE 208
           YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSE+LGQYKSKDRLAPCKLPYNE+
Sbjct: 121 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYNED 178

[Close]

BLAST of CSPI01G02550 vs. NCBI nr
Match: XP_023531696.1 (uncharacterized protein LOC111793864 [Cucurbita pepo subsp. pepo] >KAG6587563.1 hypothetical protein SDJN03_16128, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 367.9 bits (943), Expect = 6.0e-98
Identity = 171/186 (91.94%), Postives = 178/186 (95.70%), Query Frame = 0


Query: 24  DGVPTPMEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPER 83
           DGV TPMEATSSSPSPTRSRGG R SGSG+G+ERPRFFD KAKA CWAKAD VPGRHPER
Sbjct: 6   DGVETPMEATSSSPSPTRSRGGPRQSGSGNGNERPRFFDTKAKAICWAKADTVPGRHPER 65

Query: 84  WRKDAAGNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVD 143
           WRKDAAGNVVCKRFCNCQGCLCFEYDHIVP+SKGGESTADNCQILQTRVNRFKS+KDDVD
Sbjct: 66  WRKDAAGNVVCKRFCNCQGCLCFEYDHIVPFSKGGESTADNCQILQTRVNRFKSDKDDVD 125

Query: 144 TSELKSYSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLP 203
           TS+LK YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSE+LGQYKSKDRLAPCKLP
Sbjct: 126 TSQLKGYSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLP 185

Query: 204 YNEEGS 210
           YNE+ S
Sbjct: 186 YNEDSS 191

[Close]

BLAST of CSPI01G02550 vs. NCBI nr
Match: XP_022933741.1 (uncharacterized protein LOC111441065 [Cucurbita moschata] >XP_022972986.1 uncharacterized protein LOC111471509 [Cucurbita maxima])

HSP 1 Score: 367.9 bits (943), Expect = 6.0e-98
Identity = 171/186 (91.94%), Postives = 178/186 (95.70%), Query Frame = 0


Query: 24  DGVPTPMEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPER 83
           DGV TPMEATSSSPSPTRSRGG R SGSG+G+ERPRFFD KAKA CWAKAD VPGRHPER
Sbjct: 6   DGVETPMEATSSSPSPTRSRGGPRQSGSGNGNERPRFFDTKAKAICWAKADTVPGRHPER 65

Query: 84  WRKDAAGNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVD 143
           WRKDAAGNVVCKRFCNCQGCLCFEYDHIVP+SKGGESTADNCQILQTRVNRFKS+KDDVD
Sbjct: 66  WRKDAAGNVVCKRFCNCQGCLCFEYDHIVPFSKGGESTADNCQILQTRVNRFKSDKDDVD 125

Query: 144 TSELKSYSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLP 203
           TS+LK YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSE+LGQYKSKDRLAPCKLP
Sbjct: 126 TSQLKGYSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLP 185

Query: 204 YNEEGS 210
           YNE+ S
Sbjct: 186 YNEDSS 191

[Close]

BLAST of CSPI01G02550 vs. NCBI nr
Match: XP_008453223.1 (PREDICTED: uncharacterized protein LOC103494011 isoform X1 [Cucumis melo])

HSP 1 Score: 367.5 bits (942), Expect = 7.9e-98
Identity = 175/182 (96.15%), Postives = 177/182 (97.25%), Query Frame = 0


Query: 30  MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 89
           MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA
Sbjct: 1   MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 60

Query: 90  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKS 149
           GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELK 
Sbjct: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKG 120

Query: 150 YSCDVKFT----DKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLPYN 208
           YSCDVKFT    DKELDIIEMAVYGDVIRPGNQCRCRTVSE+LGQYKSKDRLAPCKLPYN
Sbjct: 121 YSCDVKFTGFLADKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYN 180

[Close]

BLAST of CSPI01G02550 vs. TAIR 10
Match: AT1G18680.1 (HNH endonuclease domain-containing protein )

HSP 1 Score: 265.0 bits (676), Expect = 5.1e-71
Identity = 120/176 (68.18%), Postives = 142/176 (80.68%), Query Frame = 0


Query: 28  TPMEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKD 87
           T     ++ P P+      R +   +G+ERPRFFD KAK  CWA AD+VPGRHPERWRKD
Sbjct: 10  TNSTTAAAVPPPSPPSSPSRSNVKSNGEERPRFFDGKAKNKCWANADIVPGRHPERWRKD 69

Query: 88  AAGNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSEL 147
            AGN+VCKRF NC GCLCFEYDHIVPYSKGGES A+NCQILQTRVNRFKS +++VD + L
Sbjct: 70  VAGNIVCKRFGNCNGCLCFEYDHIVPYSKGGESIAENCQILQTRVNRFKSAQENVDATTL 129

Query: 148 KSYSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLP 204
           KSYSC ++FTDKELD+IEMAVYGDV+RPG +CRC+TV+E+LGQ KSKD  A C+LP
Sbjct: 130 KSYSCGLQFTDKELDVIEMAVYGDVLRPGKECRCKTVAELLGQSKSKDGKAACELP 185

[Close]

BLAST of CSPI01G02550 vs. TAIR 10
Match: AT3G47490.1 (HNH endonuclease )

HSP 1 Score: 144.1 bits (362), Expect = 1.3e-34
Identity = 75/176 (42.61%), Postives = 96/176 (54.55%), Query Frame = 0


Query: 27  PTPMEATSSSPSPTRSRGGQRDS------------------GSGSGD--ERPRFFDAKAK 86
           P+      SS SP R+ G  R                    GSG GD    PR F    K
Sbjct: 57  PSKTRPELSSGSPNRNTGEARGGKVKTSATLLDREEMGLFPGSGYGDPNPEPRSFPYSVK 116

Query: 87  ASCWAKADVVPGRHPERWRKDAAGNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQ 146
             CW KA+ + GR PERWR+D  GN+V ++   C GCLC +YDHIVPYSKGG+ST +NCQ
Sbjct: 117 QQCWEKAEKIKGRDPERWRRDHLGNIVFRKLVGCPGCLCHDYDHIVPYSKGGKSTLENCQ 176

Query: 147 ILQTRVNRFKSNKDDVDTSELKSYSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCR 183
           +LQ +VNR K NK D+  SEL   S   +   +++D+IE+  YG+V R      CR
Sbjct: 177 VLQAKVNRSKGNKTDISRSELIQRSSYCRVAGRDMDLIELTAYGNVQRAPTSSGCR 232

[Close]

BLAST of CSPI01G02550 vs. TAIR 10
Match: AT3G47490.3 (HNH endonuclease )

HSP 1 Score: 132.1 bits (331), Expect = 5.2e-31
Identity = 75/196 (38.27%), Postives = 96/196 (48.98%), Query Frame = 0


Query: 27  PTPMEATSSSPSPTRSRGGQRDS------------------GSGSGD--ERPRFFDAKAK 86
           P+      SS SP R+ G  R                    GSG GD    PR F    K
Sbjct: 18  PSKTRPELSSGSPNRNTGEARGGKVKTSATLLDREEMGLFPGSGYGDPNPEPRSFPYSVK 77

Query: 87  ASCWAKADVVPGRHPERWRKDAAGNVVCKRFCNCQGCLCFEYDHIVPYS----------- 146
             CW KA+ + GR PERWR+D  GN+V ++   C GCLC +YDHIVPYS           
Sbjct: 78  QQCWEKAEKIKGRDPERWRRDHLGNIVFRKLVGCPGCLCHDYDHIVPYSKDMCPLEHAFD 137

Query: 147 ---------KGGESTADNCQILQTRVNRFKSNKDDVDTSELKSYSCDVKFTDKELDIIEM 183
                    KGG+ST +NCQ+LQ +VNR K NK D+  SEL   S   +   +++D+IE+
Sbjct: 138 EPHKDFLDLKGGKSTLENCQVLQAKVNRSKGNKTDISRSELIQRSSYCRVAGRDMDLIEL 197

[Close]

BLAST of CSPI01G02550 vs. TAIR 10
Match: AT3G47490.2 (HNH endonuclease )

HSP 1 Score: 85.5 bits (210), Expect = 5.6e-17
Identity = 46/110 (41.82%), Postives = 55/110 (50.00%), Query Frame = 0


Query: 27  PTPMEATSSSPSPTRSRGGQRDS------------------GSGSGD--ERPRFFDAKAK 86
           P+      SS SP R+ G  R                    GSG GD    PR F    K
Sbjct: 57  PSKTRPELSSGSPNRNTGEARGGKVKTSATLLDREEMGLFPGSGYGDPNPEPRSFPYSVK 116

Query: 87  ASCWAKADVVPGRHPERWRKDAAGNVVCKRFCNCQGCLCFEYDHIVPYSK 117
             CW KA+ + GR PERWR+D  GN+V ++   C GCLC +YDHIVPYSK
Sbjct: 117 QQCWEKAEKIKGRDPERWRRDHLGNIVFRKLVGCPGCLCHDYDHIVPYSK 166

The following BLAST results are available for this feature:

BLAST of CSPI01G02550 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI01G02550 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LUK3	5.0e-122	99.52	HNHc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G011480 PE=4 S...	[more]
A0A5A7UWI1	7.0e-100	98.31	HNH endonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002...	[more]
A0A1S3BVP6	7.0e-100	98.31	uncharacterized protein LOC103494011 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1I7G1	2.9e-98	91.94	uncharacterized protein LOC111471509 OS=Cucurbita maxima OX=3661 GN=LOC111471509...	[more]
A0A6J1EZW3	2.9e-98	91.94	uncharacterized protein LOC111441065 OS=Cucurbita moschata OX=3662 GN=LOC1114410...	[more]

BLAST of CSPI01G02550 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004138181.1	5.3e-102	99.45	uncharacterized protein LOC101204599 [Cucumis sativus] >KAE8652425.1 hypothetica...	[more]
XP_008453224.1	1.4e-99	98.31	PREDICTED: uncharacterized protein LOC103494011 isoform X2 [Cucumis melo] >KAA00...	[more]
XP_023531696.1	6.0e-98	91.94	uncharacterized protein LOC111793864 [Cucurbita pepo subsp. pepo] >KAG6587563.1 ...	[more]
XP_022933741.1	6.0e-98	91.94	uncharacterized protein LOC111441065 [Cucurbita moschata] >XP_022972986.1 unchar...	[more]
XP_008453223.1	7.9e-98	96.15	PREDICTED: uncharacterized protein LOC103494011 isoform X1 [Cucumis melo]	[more]

BLAST of CSPI01G02550 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G18680.1	5.1e-71	68.18	HNH endonuclease domain-containing protein	[more]
AT3G47490.1	1.3e-34	42.61	HNH endonuclease	[more]
AT3G47490.3	5.2e-31	38.27	HNH endonuclease	[more]
AT3G47490.2	5.6e-17	41.82	HNH endonuclease	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003615	HNH nuclease	SMART	SM00507	HNH_5	coord: 80..134 e-value: 0.0083 score: 25.3
IPR003615	HNH nuclease	CDD	cd00085	HNHc	coord: 95..134 e-value: 1.40115E-5 score: 39.3772
None	No IPR available	GENE3D	1.10.30.50		coord: 75..160 e-value: 8.5E-9 score: 36.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 31..49
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 31..58
None	No IPR available	PANTHER	PTHR33427:SF1	F6A14.21 PROTEIN	coord: 35..206
None	No IPR available	PANTHER	PTHR33427	HNH ENDONUCLEASE	coord: 35..206
IPR002711	HNH endonuclease	PFAM	PF01844	HNH	coord: 94..128 e-value: 4.7E-5 score: 23.5

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G02550.1	CSPI01G02550.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
molecular_function	GO:0004519	endonuclease activity
molecular_function	GO:0003676	nucleic acid binding