CSPI01G02460 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G02460
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	PMEI domain-containing protein
Location	Chr1: 1556646 .. 1557278 (+)
RNA-Seq Expression	CSPI01G02460
Synteny	CSPI01G02460

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AACAAAACATCCTTCTTCCTAAAACTATTAGCCAAAGAAATAATAAAAAAAAAAGTTATGATGATCATGATCTCTACTTCCTTTCTCTTCTCCTTCACTATTGTCTTTATACTCATTTTTTCCTCATTCTCCCAAACCTATTCAAATCCCCACAATATCATTCAAGAAACATGCAAGAAATCAGCTGCGAGTACCCCAAATCTCACCTACAAATTCTGTGTTACTTCTTTAGAATCCGACACCCGTAGTCGCTATGCGAATCTTCACAAACTTGGCCTCATTTCCATGGACTTGTTGCGACACAACGTAACAAGCACTCGACGAGAAATCAAAAAGCTTTTGAGGAACAAAAAAATGGAAGAGTTCATCAAGGGTTGTTTGAAGGATTGTGTAGAGCTTTATTCTGATGCTGTTCCAACATTGAAAGAAGCAAAAAGAGAATACAAAAATAGGAATTATAAAGATGCAAATATTAAGGTAAGTTCAATTATGGAAGCTCCTACAACTTGTGAAAATGGGTTTAAAGAAAAAGAAGGAATCATTTCGCCATTGACAAAGAATAACAGTGATGTGTTTCAATTGGCAGCTTTGACTCTCTCCATCATCAATATGAATCTGCATGATATCCAATAA

mRNA sequence

AACAAAACATCCTTCTTCCTAAAACTATTAGCCAAAGAAATAATAAAAAAAAAAGTTATGATGATCATGATCTCTACTTCCTTTCTCTTCTCCTTCACTATTGTCTTTATACTCATTTTTTCCTCATTCTCCCAAACCTATTCAAATCCCCACAATATCATTCAAGAAACATGCAAGAAATCAGCTGCGAGTACCCCAAATCTCACCTACAAATTCTGTGTTACTTCTTTAGAATCCGACACCCGTAGTCGCTATGCGAATCTTCACAAACTTGGCCTCATTTCCATGGACTTGTTGCGACACAACGTAACAAGCACTCGACGAGAAATCAAAAAGCTTTTGAGGAACAAAAAAATGGAAGAGTTCATCAAGGGTTGTTTGAAGGATTGTGTAGAGCTTTATTCTGATGCTGTTCCAACATTGAAAGAAGCAAAAAGAGAATACAAAAATAGGAATTATAAAGATGCAAATATTAAGGTAAGTTCAATTATGGAAGCTCCTACAACTTGTGAAAATGGGTTTAAAGAAAAAGAAGGAATCATTTCGCCATTGACAAAGAATAACAGTGATGTGTTTCAATTGGCAGCTTTGACTCTCTCCATCATCAATATGAATCTGCATGATATCCAATAA

Coding sequence (CDS)

ATGATGATCATGATCTCTACTTCCTTTCTCTTCTCCTTCACTATTGTCTTTATACTCATTTTTTCCTCATTCTCCCAAACCTATTCAAATCCCCACAATATCATTCAAGAAACATGCAAGAAATCAGCTGCGAGTACCCCAAATCTCACCTACAAATTCTGTGTTACTTCTTTAGAATCCGACACCCGTAGTCGCTATGCGAATCTTCACAAACTTGGCCTCATTTCCATGGACTTGTTGCGACACAACGTAACAAGCACTCGACGAGAAATCAAAAAGCTTTTGAGGAACAAAAAAATGGAAGAGTTCATCAAGGGTTGTTTGAAGGATTGTGTAGAGCTTTATTCTGATGCTGTTCCAACATTGAAAGAAGCAAAAAGAGAATACAAAAATAGGAATTATAAAGATGCAAATATTAAGGTAAGTTCAATTATGGAAGCTCCTACAACTTGTGAAAATGGGTTTAAAGAAAAAGAAGGAATCATTTCGCCATTGACAAAGAATAACAGTGATGTGTTTCAATTGGCAGCTTTGACTCTCTCCATCATCAATATGAATCTGCATGATATCCAATAA

Protein sequence

MMIMISTSFLFSFTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTRSRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLSIINMNLHDIQ*

Homology

[Close]

BLAST of CSPI01G02460 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 5.6e-29
Identity = 65/153 (42.48%), Postives = 106/153 (69.28%), Query Frame = 0


Query: 33  NIIQETCKKSAASTPNLTYKFCVTSLESDTRSRYANLHKLGLISMDLLRHNVTSTRREIK 92
           +I+Q TCKK A  +PN+ Y FCV SL +D +S  A+L  LG+IS +L   + +  +  I 
Sbjct: 25  DIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHTADLQGLGVISANLAIQHGSKIQTFIG 84

Query: 93  KLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKNRNYKDANIKVSSIMEAPTTCE 152
           ++L++ K++  +K  L DCV LY+DA  +++EA  ++K+++Y  AN+K+S+ ++   TCE
Sbjct: 85  RILKS-KVDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDSVTCE 144

Query: 153 NGFKEKEGIISPLTKNNSDVFQLAALTLSIINM 186
           +GFKEK+GI+SP+TK N D  QL A++L+I  +
Sbjct: 145 DGFKEKKGIVSPVTKENKDYVQLTAISLAITKL 176

[Close]

BLAST of CSPI01G02460 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.1e-28
Identity = 65/153 (42.48%), Postives = 104/153 (67.97%), Query Frame = 0


Query: 33  NIIQETCKKSAASTPNLTYKFCVTSLESDTRSRYANLHKLGLISMDLLRHNVTSTRREIK 92
           +I+Q TCKK A  +PN+ Y FCV SL +D +S  A+L  LG+IS +L     +  +  I 
Sbjct: 16  DIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHSADLQGLGVISANLAIQQGSKIQTFIG 75

Query: 93  KLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKNRNYKDANIKVSSIMEAPTTCE 152
           ++L++ K++  +K  L DCV LY+DA  +++EA  ++K+++Y  AN+K+S+ ++   TCE
Sbjct: 76  RILKS-KVDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDSVTCE 135

Query: 153 NGFKEKEGIISPLTKNNSDVFQLAALTLSIINM 186
           +GFKEK+GI SP+TK N D  QL A++L+I  +
Sbjct: 136 DGFKEKKGIASPVTKENKDYVQLTAISLAITKL 167

[Close]

BLAST of CSPI01G02460 vs. ExPASy Swiss-Prot
Match: Q9FJR5 (Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 6.9e-19
Identity = 58/172 (33.72%), Postives = 99/172 (57.56%), Query Frame = 0


Query: 15  IVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTRSRYA-NLHKLG 74
           +V ++IFS F   ++    +IQ++CKK+ A  P L+Y FCV SL  D +S+ A  L  L 
Sbjct: 4   LVSLVIFSLFLNGFATAQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESLV 63

Query: 75  LISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKNRN 134
           L S       +T+ +  + + L++++ ++ ++  LK C+  Y+DA   L  A    K+R+
Sbjct: 64  LASTKTAAAKITNLKGIVAQDLKDQRYQDIVED-LKLCLGFYNDANDDLTTALANIKSRD 123

Query: 135 YKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLSIINM 186
           Y+ ANI +S+ ++ P  CE+ FKE +   SP+T  NS +F+   + L+  NM
Sbjct: 124 YQGANINLSAALDVPGNCEDDFKEAKK-TSPITNENSILFKTILIPLAFTNM 173

[Close]

BLAST of CSPI01G02460 vs. ExPASy Swiss-Prot
Match: O49603 (Cell wall / vacuolar inhibitor of fructosidase 2 OS=Arabidopsis thaliana OX=3702 GN=C/VIF2 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 6.5e-09
Identity = 48/161 (29.81%), Postives = 78/161 (48.45%), Query Frame = 0


Query: 5   ISTSFLFSFTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTRS 64
           +++S +F   +      S+     SN   II+ TCK     T N  YKFCV++L+SD RS
Sbjct: 1   MASSLIFLLLVTLTFSASTLISAKSNTTTIIESTCK-----TTNY-YKFCVSALKSDPRS 60

Query: 65  RYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKE 124
             A+   L  I + +   N TST   I   L     +  +K  L+DC E Y+ A  +L+ 
Sbjct: 61  PTADTKGLASIMVGVGMTNATSTANYIAGNLSATVKDTVLKKVLQDCSEKYALAADSLRL 120

Query: 125 AKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPL 166
             ++  +  Y  A++ V +  + P  C N F+  +G+  P+
Sbjct: 121 TIQDLDDEAYDYASMHVLAAQDYPNVCRNIFRRVKGLAYPV 155

[Close]

BLAST of CSPI01G02460 vs. ExPASy Swiss-Prot
Match: P83326 (Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2)

HSP 1 Score: 62.0 bits (149), Expect = 8.5e-09
Identity = 55/187 (29.41%), Postives = 93/187 (49.73%), Query Frame = 0


Query: 1   MMIMISTSFLFSFTIVFILIFSSFSQ--TYSNPHNIIQETCKKSAASTPNLTYKFCVTSL 60
           M     +S LF   ++ IL  S  SQ  +    +++I E C K+    P+L    C+ +L
Sbjct: 1   MAFSYCSSSLFVSLLLVILFISPLSQRPSVKAENHLISEICPKT--RNPSL----CLQAL 60

Query: 61  ESDTRSRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDA 120
           ESD RS   +L  LG  S+D+ + +   T + I   L N+  +  +KG  + C E Y+DA
Sbjct: 61  ESDPRSASKDLKGLGQFSIDIAQASAKQTSKIIAS-LTNQATDPKLKGRYETCSENYADA 120

Query: 121 VPTLKEAKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAAL 180
           + +L +AK+   + +Y   NI  S+  +   TCE+ F+    I + L + +  +  L  +
Sbjct: 121 IDSLGQAKQFLTSGDYNSLNIYASAAFDGAGTCEDSFEGPPNIPTQLHQADLKLEDLCDI 180

Query: 181 TLSIINM 186
            L I N+
Sbjct: 181 VLVISNL 180

[Close]

BLAST of CSPI01G02460 vs. ExPASy TrEMBL
Match: A0A0A0LUJ3 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G009900 PE=4 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 6.6e-97
Identity = 188/188 (100.00%), Postives = 188/188 (100.00%), Query Frame = 0


Query: 4   MISTSFLFSFTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTR 63
           MISTSFLFSFTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTR
Sbjct: 1   MISTSFLFSFTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTR 60

Query: 64  SRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLK 123
           SRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLK
Sbjct: 61  SRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLK 120

Query: 124 EAKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLSII 183
           EAKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLSII
Sbjct: 121 EAKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLSII 180

Query: 184 NMNLHDIQ 192
           NMNLHDIQ
Sbjct: 181 NMNLHDIQ 188

[Close]

BLAST of CSPI01G02460 vs. ExPASy TrEMBL
Match: A0A6J1EZS0 (putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111440851 PE=4 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 6.0e-66
Identity = 129/185 (69.73%), Postives = 159/185 (85.95%), Query Frame = 0


Query: 4   MISTSFLFSFTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTR 63
           MIST  LF  + + +++ SSFS   S PHN++QETCKK AA+TPNL+Y FCV+SLESD R
Sbjct: 1   MISTFSLFFLSPLLVILVSSFSHG-STPHNMVQETCKKCAATTPNLSYAFCVSSLESDDR 60

Query: 64  SRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLK 123
           SR A+LH+LGLISMDLLRHN+TSTRRE+KKLLRNK ++ F+K CL DC+ELYSDA+PTLK
Sbjct: 61  SRSASLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKACLDDCLELYSDAIPTLK 120

Query: 124 EAKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLSII 183
           E KREYK + +  AN+KVSSIMEAP+TCE+GF EK+G++SPLTKNN +VFQLAALTLSI+
Sbjct: 121 ECKREYKQKRFSGANVKVSSIMEAPSTCEDGFHEKQGVVSPLTKNNKNVFQLAALTLSIV 180

Query: 184 NMNLH 189
           NMN+H
Sbjct: 181 NMNMH 184

[Close]

BLAST of CSPI01G02460 vs. ExPASy TrEMBL
Match: A0A6J1I7I4 (putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111471526 PE=4 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 4.3e-64
Identity = 129/185 (69.73%), Postives = 158/185 (85.41%), Query Frame = 0


Query: 4   MISTSFLFSFTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTR 63
           MIST  LF  + +F+++ SS S   S PHN++QETCKK AA+TPNL+Y FCV+SLESD R
Sbjct: 1   MISTFSLFFLSPLFVILVSSSSHG-STPHNMVQETCKKCAATTPNLSYSFCVSSLESDDR 60

Query: 64  SRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLK 123
           SR ANLH+LGLISMDLLRHN+TSTRRE+KKLLRNK ++ F+K  L DC+ELYSDA+PTLK
Sbjct: 61  SRSANLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKVRLDDCLELYSDAIPTLK 120

Query: 124 EAKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLSII 183
           E KREYK + + DAN+KVSSIMEAP+TCE+GF EK+G+ SPLTK+N +VFQLAALTLSI+
Sbjct: 121 ECKREYKQKRFSDANVKVSSIMEAPSTCEDGFHEKQGVGSPLTKSNKNVFQLAALTLSIV 180

Query: 184 NMNLH 189
           NMN+H
Sbjct: 181 NMNMH 184

[Close]

BLAST of CSPI01G02460 vs. ExPASy TrEMBL
Match: A0A6J1C4E5 (putative invertase inhibitor OS=Momordica charantia OX=3673 GN=LOC111007285 PE=4 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 2.0e-61
Identity = 128/187 (68.45%), Postives = 156/187 (83.42%), Query Frame = 0


Query: 3   IMISTSFLFSFTIVFILIFSSFSQ-TYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESD 62
           IMIST   FS T  F ++F SFS  +     NIIQETCK+SA STPNL+Y FCV SLESD
Sbjct: 26  IMIST---FSLTTFFAVLFFSFSDGSTITTSNIIQETCKRSADSTPNLSYNFCVASLESD 85

Query: 63  TRSRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPT 122
            RSR A+LHKLGLI+MDL+RHN+TSTRR+I+KLLRNKK+++F+K  L DC+ELYSDAVP 
Sbjct: 86  DRSRCADLHKLGLIAMDLMRHNITSTRRDIRKLLRNKKLDQFVKARLDDCLELYSDAVPI 145

Query: 123 LKEAKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLS 182
           LKEAKR YK + Y DAN+KVSS+MEAP+TCE+GF++KEG++SPLT+ N +VF+LAALTLS
Sbjct: 146 LKEAKRCYKEKKYVDANVKVSSVMEAPSTCEDGFRDKEGLVSPLTEKNKNVFRLAALTLS 205

Query: 183 IINMNLH 189
           IINMN H
Sbjct: 206 IINMNTH 209

[Close]

BLAST of CSPI01G02460 vs. ExPASy TrEMBL
Match: A0A2K1XQD5 (PMEI domain-containing protein OS=Populus trichocarpa OX=3694 GN=POPTR_014G044100 PE=4 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 3.7e-47
Identity = 94/176 (53.41%), Postives = 140/176 (79.55%), Query Frame = 0


Query: 11  FSFTIVFILIFSSFSQTYSN-PHNIIQETCKKSAASTPNLTYKFCVTSLESDTRSRYANL 70
           FS+ ++ + +   F   ++N   N+I ETCKK AA+ PNL+Y FCV+SL++  RS+  NL
Sbjct: 4   FSYCLLLLFLPLLFCTFHANIAQNLIPETCKKCAANDPNLSYNFCVSSLQASNRSQCDNL 63

Query: 71  HKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREY 130
             LG++S+ L+++NVT+TR  +K LL+NKKM+ FI+ CL DC++LYSDA+PTLK+A  +Y
Sbjct: 64  RGLGMMSIKLIKYNVTNTRHYVKNLLKNKKMDPFIRACLNDCLDLYSDAIPTLKQAMIDY 123

Query: 131 KNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLSIINM 186
           K+++YKDANI+VSS+++A TTCE+GF++KEG +SPLTK N+D FQL+A+ L++INM
Sbjct: 124 KSKHYKDANIEVSSVIDAATTCEDGFEDKEGAVSPLTKRNNDTFQLSAIALALINM 179

[Close]

BLAST of CSPI01G02460 vs. NCBI nr
Match: KAE8652421.1 (hypothetical protein Csa_013805 [Cucumis sativus])

HSP 1 Score: 307.0 bits (785), Expect = 1.2e-79
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 0


Query: 37  ETCKKSAASTPNLTYKFCVTSLESDTRSRYANLHKLGLISMDLLRHNVTSTRREIKKLLR 96
           ETCKKSAASTPNLTYKFCVTSLESDTRSRYANLHKLGLISMDLLRHNVTSTRREIKKLLR
Sbjct: 5   ETCKKSAASTPNLTYKFCVTSLESDTRSRYANLHKLGLISMDLLRHNVTSTRREIKKLLR 64

Query: 97  NKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKNRNYKDANIKVSSIMEAPTTCENGFK 156
           NKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKNRNYKDANIKVSSIMEAPTTCENGFK
Sbjct: 65  NKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKNRNYKDANIKVSSIMEAPTTCENGFK 124

Query: 157 EKEGIISPLTKNNSDVFQLAALTLSIINMNLHDIQ 192
           EKEGIISPLTKNNSDVFQLAALTLSIINMNLHDIQ
Sbjct: 125 EKEGIISPLTKNNSDVFQLAALTLSIINMNLHDIQ 159

[Close]

BLAST of CSPI01G02460 vs. NCBI nr
Match: XP_038880152.1 (putative invertase inhibitor [Benincasa hispida])

HSP 1 Score: 295.0 bits (754), Expect = 4.5e-76
Identity = 153/187 (81.82%), Postives = 168/187 (89.84%), Query Frame = 0


Query: 2   MIMISTSFLFSFTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESD 61
           MIMISTS LFSFT   +LIFSS S   SN HNIIQETCKKSA S+PN++YKFCV S ESD
Sbjct: 1   MIMISTSHLFSFTAFLLLIFSSSSHG-SNTHNIIQETCKKSAESSPNISYKFCVASFESD 60

Query: 62  TRSRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPT 121
            RSR ANLHKLGLISMDL+RHN+TSTRREIKKLLRNKK+EEFIKGCLKDC+ELYSDAVPT
Sbjct: 61  NRSRSANLHKLGLISMDLMRHNITSTRREIKKLLRNKKLEEFIKGCLKDCLELYSDAVPT 120

Query: 122 LKEAKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLS 181
           LKEAKREYK + YKDAN+KVSSIMEAPTTCE+GF+EK+G ISPLT NNS+ FQLAALTLS
Sbjct: 121 LKEAKREYKEKKYKDANVKVSSIMEAPTTCEDGFQEKQGTISPLTNNNSNAFQLAALTLS 180

Query: 182 IINMNLH 189
           IINMN+H
Sbjct: 181 IINMNMH 186

[Close]

BLAST of CSPI01G02460 vs. NCBI nr
Match: XP_022933437.1 (putative invertase inhibitor [Cucurbita moschata])

HSP 1 Score: 260.4 bits (664), Expect = 1.2e-65
Identity = 129/185 (69.73%), Postives = 159/185 (85.95%), Query Frame = 0


Query: 4   MISTSFLFSFTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTR 63
           MIST  LF  + + +++ SSFS   S PHN++QETCKK AA+TPNL+Y FCV+SLESD R
Sbjct: 1   MISTFSLFFLSPLLVILVSSFSHG-STPHNMVQETCKKCAATTPNLSYAFCVSSLESDDR 60

Query: 64  SRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLK 123
           SR A+LH+LGLISMDLLRHN+TSTRRE+KKLLRNK ++ F+K CL DC+ELYSDA+PTLK
Sbjct: 61  SRSASLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKACLDDCLELYSDAIPTLK 120

Query: 124 EAKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLSII 183
           E KREYK + +  AN+KVSSIMEAP+TCE+GF EK+G++SPLTKNN +VFQLAALTLSI+
Sbjct: 121 ECKREYKQKRFSGANVKVSSIMEAPSTCEDGFHEKQGVVSPLTKNNKNVFQLAALTLSIV 180

Query: 184 NMNLH 189
           NMN+H
Sbjct: 181 NMNMH 184

[Close]

BLAST of CSPI01G02460 vs. NCBI nr
Match: XP_022973011.1 (putative invertase inhibitor [Cucurbita maxima])

HSP 1 Score: 254.2 bits (648), Expect = 8.9e-64
Identity = 129/185 (69.73%), Postives = 158/185 (85.41%), Query Frame = 0


Query: 4   MISTSFLFSFTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTR 63
           MIST  LF  + +F+++ SS S   S PHN++QETCKK AA+TPNL+Y FCV+SLESD R
Sbjct: 1   MISTFSLFFLSPLFVILVSSSSHG-STPHNMVQETCKKCAATTPNLSYSFCVSSLESDDR 60

Query: 64  SRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLK 123
           SR ANLH+LGLISMDLLRHN+TSTRRE+KKLLRNK ++ F+K  L DC+ELYSDA+PTLK
Sbjct: 61  SRSANLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKVRLDDCLELYSDAIPTLK 120

Query: 124 EAKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLSII 183
           E KREYK + + DAN+KVSSIMEAP+TCE+GF EK+G+ SPLTK+N +VFQLAALTLSI+
Sbjct: 121 ECKREYKQKRFSDANVKVSSIMEAPSTCEDGFHEKQGVGSPLTKSNKNVFQLAALTLSIV 180

Query: 184 NMNLH 189
           NMN+H
Sbjct: 181 NMNMH 184

[Close]

BLAST of CSPI01G02460 vs. NCBI nr
Match: XP_023531537.1 (putative invertase inhibitor [Cucurbita pepo subsp. pepo])

HSP 1 Score: 245.7 bits (626), Expect = 3.2e-61
Identity = 116/155 (74.84%), Postives = 140/155 (90.32%), Query Frame = 0


Query: 34  IIQETCKKSAASTPNLTYKFCVTSLESDTRSRYANLHKLGLISMDLLRHNVTSTRREIKK 93
           ++QETCKK AA+TPNL+Y FCV+SLESD RSR ANLH+LGLISMDLLRHN+TSTRRE+KK
Sbjct: 1   MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSANLHRLGLISMDLLRHNLTSTRREVKK 60

Query: 94  LLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKNRNYKDANIKVSSIMEAPTTCEN 153
           LLRNK ++ F+K  L DC+ELYSDA+PTLKE KREYK + + DAN+KVSSIMEAP+TCE+
Sbjct: 61  LLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRFNDANVKVSSIMEAPSTCED 120

Query: 154 GFKEKEGIISPLTKNNSDVFQLAALTLSIINMNLH 189
           GF EK+G++SPLTKNN +VFQLAALTLSI+NMN+H
Sbjct: 121 GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMH 155

[Close]

BLAST of CSPI01G02460 vs. TAIR 10
Match: AT5G46970.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 110.5 bits (275), Expect = 1.5e-24
Identity = 66/168 (39.29%), Postives = 104/168 (61.90%), Query Frame = 0


Query: 8   SFLFSFTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTRSRYA 67
           +FLF F + F+L+       ++   ++IQ+ CKK+A   P + Y FCV SLE + +S+ A
Sbjct: 2   TFLFYFAMFFLLL-----NAFTIAQSLIQDFCKKAADKNPKIHYNFCVKSLEENPQSKTA 61

Query: 68  -NLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAK 127
            +L +L ++S        TS +  + K+L+  + E + +  L+DC+ELYSDA  +LKEA 
Sbjct: 62  RSLDRLVMLSTKNAVSKTTSMKGIVDKILKENRFEMYSEKPLRDCLELYSDATNSLKEAL 121

Query: 128 REYKNRNYKDANIKVSSIMEAPTTCENGFKE-KEGIISPLTKNNSDVF 174
              K+R+YK AN+ +S+ M AP +CE GFKE K+ + SP TK+N  +F
Sbjct: 122 TIIKSRDYKTANVVISAAMGAPPSCEIGFKEGKKPLKSPFTKDNDVLF 164

[Close]

BLAST of CSPI01G02460 vs. TAIR 10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 106.7 bits (265), Expect = 2.1e-23
Identity = 57/172 (33.14%), Postives = 104/172 (60.47%), Query Frame = 0


Query: 15  IVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTRSRYA-NLHKLG 74
           +++++ F   S   +N   +I+ +CKK+ A++P   Y  CVTSLE++ +++ A +L  L 
Sbjct: 4   LLYLVTFFVLSNGLANGQTLIRNSCKKATATSPKFKYNLCVTSLETNPQAKTAKDLAGLV 63

Query: 75  LISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKNRN 134
           + S        T+ +  + K+++ KK+ +     L+DC++LY+DA+ +L EA    K+RN
Sbjct: 64  MASTKNAVTKATTLKGTVDKIIKGKKVNKMTAMPLRDCLQLYTDAIGSLNEALAGVKSRN 123

Query: 135 YKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLSIINM 186
           Y      +S+ M+ P+TCE GFKE++   SP+TK N +++Q+  + L+  NM
Sbjct: 124 YPTVKTVLSAAMDTPSTCETGFKERKA-PSPVTKENDNLYQMILIPLAFTNM 174

[Close]

BLAST of CSPI01G02460 vs. TAIR 10
Match: AT5G46960.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 95.5 bits (236), Expect = 4.9e-20
Identity = 58/172 (33.72%), Postives = 99/172 (57.56%), Query Frame = 0


Query: 15  IVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTRSRYA-NLHKLG 74
           +V ++IFS F   ++    +IQ++CKK+ A  P L+Y FCV SL  D +S+ A  L  L 
Sbjct: 4   LVSLVIFSLFLNGFATAQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESLV 63

Query: 75  LISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKNRN 134
           L S       +T+ +  + + L++++ ++ ++  LK C+  Y+DA   L  A    K+R+
Sbjct: 64  LASTKTAAAKITNLKGIVAQDLKDQRYQDIVED-LKLCLGFYNDANDDLTTALANIKSRD 123

Query: 135 YKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLSIINM 186
           Y+ ANI +S+ ++ P  CE+ FKE +   SP+T  NS +F+   + L+  NM
Sbjct: 124 YQGANINLSAALDVPGNCEDDFKEAKK-TSPITNENSILFKTILIPLAFTNM 173

[Close]

BLAST of CSPI01G02460 vs. TAIR 10
Match: AT5G46930.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 93.6 bits (231), Expect = 1.9e-19
Identity = 57/175 (32.57%), Postives = 101/175 (57.71%), Query Frame = 0


Query: 13  FTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTRSRYA-NLHK 72
           + ++F L+ + F+   S    +I+++CKK+    P L Y FCV SLE + +S+ A +L  
Sbjct: 6   YLVMFFLLLNCFATAQS----LIRDSCKKATTKDPKLKYDFCVKSLEENPQSKTAKSLEG 65

Query: 73  LGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKN 132
           L  +S        TS +  + K+L+  K E  ++  L DC+ELY++A+ +L ++    K+
Sbjct: 66  LVFVSTKNAVSKTTSLKGMVDKILKEDKYE--VERPLLDCLELYTEAIDSLNQSLDTVKS 125

Query: 133 RNYKDANIKVSSIMEAPTTCENGF-KEKEGIISPLTKNNSDVFQLAALTLSIINM 186
           R+YK A + +S+ M+AP +CE  F K K+ + SP TK N  +F +  + ++  +M
Sbjct: 126 RDYKTATMLMSAAMDAPGSCETKFTKRKKAVKSPFTKENDVLFYMVLIPIAFTSM 174

[Close]

BLAST of CSPI01G02460 vs. TAIR 10
Match: AT5G38610.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 90.9 bits (224), Expect = 1.2e-18
Identity = 67/185 (36.22%), Postives = 94/185 (50.81%), Query Frame = 0


Query: 16  VFILIFSSF--SQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESD--TRSRYANLHK 75
           VF +IF  F  SQ  S   ++I +TCK  AA +    Y FCV+SL +         NL  
Sbjct: 6   VFYIIFLFFLVSQVKST-SDMIDQTCKSCAAKSTIFDYNFCVSSLNNSPIALPSPTNLSS 65

Query: 76  LGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKN 135
           L L+ M     N T+T   I++LL +     F   CL+DC+ELY DA   L+EA R +  
Sbjct: 66  LALVPMLQALDNATATASTIQQLLISDDDGSFRSACLRDCLELYEDATDRLEEAVRVFIT 125

Query: 136 R-NYKDANIKVSSIMEAPTTCENGFKEKE-------GI---ISPLTKNNSDVFQLAALTL 186
           R      N+ VS+ ME+  TCENGF+E++       G+    SP+   N  +F+   + L
Sbjct: 126 RKELGTVNVMVSAAMESAVTCENGFRERDDGDGGGGGVTTWTSPIGDENHKLFEFGQIAL 185

The following BLAST results are available for this feature:

BLAST of CSPI01G02460 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GT41	5.6e-29	42.48	Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1	[more]
A9YUH4	2.1e-28	42.48	Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1	[more]
Q9FJR5	6.9e-19	33.72	Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1	[more]
O49603	6.5e-09	29.81	Cell wall / vacuolar inhibitor of fructosidase 2 OS=Arabidopsis thaliana OX=3702...	[more]
P83326	8.5e-09	29.41	Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2	[more]

BLAST of CSPI01G02460 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LUJ3	6.6e-97	100.00	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G009900 PE=4 S...	[more]
A0A6J1EZS0	6.0e-66	69.73	putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111440851 PE=4 ...	[more]
A0A6J1I7I4	4.3e-64	69.73	putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111471526 PE=4 SV...	[more]
A0A6J1C4E5	2.0e-61	68.45	putative invertase inhibitor OS=Momordica charantia OX=3673 GN=LOC111007285 PE=4...	[more]
A0A2K1XQD5	3.7e-47	53.41	PMEI domain-containing protein OS=Populus trichocarpa OX=3694 GN=POPTR_014G04410...	[more]

BLAST of CSPI01G02460 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8652421.1	1.2e-79	100.00	hypothetical protein Csa_013805 [Cucumis sativus]	[more]
XP_038880152.1	4.5e-76	81.82	putative invertase inhibitor [Benincasa hispida]	[more]
XP_022933437.1	1.2e-65	69.73	putative invertase inhibitor [Cucurbita moschata]	[more]
XP_022973011.1	8.9e-64	69.73	putative invertase inhibitor [Cucurbita maxima]	[more]
XP_023531537.1	3.2e-61	74.84	putative invertase inhibitor [Cucurbita pepo subsp. pepo]	[more]

BLAST of CSPI01G02460 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G46970.1	1.5e-24	39.29	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46940.1	2.1e-23	33.14	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46960.1	4.9e-20	33.72	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46930.1	1.9e-19	32.57	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G38610.1	1.2e-18	36.22	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 77..97
None	No IPR available	PANTHER	PTHR31707:SF314	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR	coord: 4..185
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 4..185
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 30..182 e-value: 3.9E-29 score: 112.8
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 8..185 e-value: 3.4E-45 score: 151.5
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 34..182 e-value: 2.5E-29 score: 102.6
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 31..186 e-value: 4.3E-40 score: 139.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 32..185
IPR034088	Pla a 1-like	CDD	cd15795	PMEI-Pla_a_1_like	coord: 39..185 e-value: 2.22369E-52 score: 162.92

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G02460.1	CSPI01G02460.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004857	enzyme inhibitor activity