CSPI01G02270 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G02270
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	protein EMBRYO DEFECTIVE 514
Location	Chr1: 1476410 .. 1478716 (+)
RNA-Seq Expression	CSPI01G02270
Synteny	CSPI01G02270

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCTTCTATACTGTACTTTGCTAGGGTTTTAGTTTGAAGCTTGATTGTTCCAACTTGAATTCACAAATTACAATGGCGGAAGAGACAATCCCTCAACTCGATCCGGAGGTAACCAACTCAGGCACTGAAGATATGGAATTGGAAACTACGGAACCGAAAGCTTCAACTGATGCTACTGAAGCAAATGAGGCCGCTCCAGTAATTAATGGCGATGCTACTTCGAAGCGAGAGAGGGAGGAAAGCGCCGATGATGGCGTCGCCGATGGGAAGAAGCAGAAGATAGAGAAATCTGTTGAGGAGGAAAGGCTTGAGAAGTTGGGTGGAGACGGTAAGTGTGAGGAGGAACCTGTTCCTGTGAGCTTAGGCCCCAAGAGCTTTCGATCATCGGTGGAATTGTTTGATTACTTCTATAAGATCCTTCACCACTGGCCTGTCAACTTGAACGTGAACCAGGTATGATATTATTAACTTATTTTAAATGTGTCATAGTTCTCTGTTTTTGTTAATTGAAAGCTCGAGAATGATATTGAGAAAATACTAATTTTGTTATAAGGGATTGTTGGAACTTGGTTGAACAGTTTTAGCAAAAGGGTTTCTATATAACTGCATGCTTATTCAAAAGGGAATCTCAAATGTCCAAGTTCTTTTTTATATTATTTGAATACAATGCTTGCTTGTTTGAATTATTTATGGTTTCCTGTTGTTGAGTATAATTTAATTGAAGACATAAAGATTGTATTGCTTCTTACTGCCAGTTAGATTAATTATTGTTGTTTGTTTAATTTGATAAAATTTGAACTCGAATGGATATTCATCTATATTGTGCTCTATTTGGTCGTGTTGTGATTCGTTTAGTTGATTCTTTTAGTAAATTTTTGTTGTTATGGCTGGATGGTTTTTCCTGAACTAAGTATGATTTTTGAGTCAGTTTCCAATATTTCTTCAAATGTTTTGTTGACGGTTGGTTGGTGTCCGTTTTACTCAAAACCTACTTACTTCTTTAGATGAGACTGTTCGACATTATTAATTATGGAAAATGGAATCTGGGTAGTAGTAGTACTAGAAAAATCCCAGCCTGGAGGGATAAATTTTATTTATTTCTGTGACGGATACTCTTTTACCTTATTTGCTCATTCATGAATTTCTTTCAGCAGTTTATTTTTCTTGTGTTATGTTGTTATTCTTCAGGATTCCCGTATTCTTGATTGATTGATGTCTGTGGAATTAATTAATGATATTGATATACTTTATCTATATACTACGGTATGGCTTATCCTGTGATGTGGAGCAAATTATTCAAACAGAAGCTATGGCTTCTAACCGTTTGTGATACAATGAGAACATTTTTATCAAATAATTCGTAATTAGAAATGCAGTCAAGTGTTTATCTTCTACTGTCTTAAAGCGTTTAGAAGTGTATTCCTTTAGGCTTTAGCTCACCTTACCATAACTTAAATGGTTAAAACATAAGCAATGCCAATTTTAGGCTTGAATTTATGTTCTATGAAGAGTGTATTTCCTTAAGGTCCCCTATATTAACTTGTAAGTCGTGCTTTTCATTTAATTAACAGCCTTTCCAACGTAAAAGATAATCCCCCGAAGTCATTGACTTGATTTGAAACATCTATAAATGATAATTTACATGATCAGAAAGCAGTGACTCTGTTAATACAATATTTGATATTTGTTTCTATGGCAGTACGAGCAAATGGTGCTGGTAGATTTACTTAAGAAGGGACATCTAGAACCAGAGAAAAAGATTGGCTGTGGTATTCACTCTTTTCAAATTCGTTTCCATCCTGAGTGGAAAAGCAAGTGCTTCTTCCTCATCCGGGAGGATGAGTCTGCTGATGACTTCAGCTTCAGGAAGTGTGTTGATCATATCCTTCCTCTGCCTGAAAACTTCAAAGGAAAATTTGATGCAAACAGAGCATTAGGCGGTGGTAAGCACCGCGGTGGAGGACGAGGTGGGGGTGGTGGACGTGGAAGAGGAGGAGGGGGTCGGTGGAGAAACTGATGTTTTTCCCAACACTTTTCAGTCTCACCACTTTGGACTCAATTTTCCTGCGAAGAATTTACCACAAAATCCCCACCAATTCAAGCTTCTTGGCCATGAGTTCAATGAAAAAAGTAGAGGAATAATTTTTTTATGCCCATAATTTATGCTTTTCTTAGCCTGCTTGATGTATGAACTCAAAATTATGATACAATTTTCTGACGATAATTGTTAAAGGATGAGTTTTTATGTTAATTTTAGAGAGAATTAATGTCCGAATATTATCCAAGACTCTTAAATTTTGTTGGTTT

mRNA sequence

GTCTTCTATACTGTACTTTGCTAGGGTTTTAGTTTGAAGCTTGATTGTTCCAACTTGAATTCACAAATTACAATGGCGGAAGAGACAATCCCTCAACTCGATCCGGAGGTAACCAACTCAGGCACTGAAGATATGGAATTGGAAACTACGGAACCGAAAGCTTCAACTGATGCTACTGAAGCAAATGAGGCCGCTCCAGTAATTAATGGCGATGCTACTTCGAAGCGAGAGAGGGAGGAAAGCGCCGATGATGGCGTCGCCGATGGGAAGAAGCAGAAGATAGAGAAATCTGTTGAGGAGGAAAGGCTTGAGAAGTTGGGTGGAGACGGTAAGTGTGAGGAGGAACCTGTTCCTGTGAGCTTAGGCCCCAAGAGCTTTCGATCATCGGTGGAATTGTTTGATTACTTCTATAAGATCCTTCACCACTGGCCTGTCAACTTGAACGTGAACCAGTACGAGCAAATGGTGCTGGTAGATTTACTTAAGAAGGGACATCTAGAACCAGAGAAAAAGATTGGCTGTGGTATTCACTCTTTTCAAATTCGTTTCCATCCTGAGTGGAAAAGCAAGTGCTTCTTCCTCATCCGGGAGGATGAGTCTGCTGATGACTTCAGCTTCAGGAAGTGTGTTGATCATATCCTTCCTCTGCCTGAAAACTTCAAAGGAAAATTTGATGCAAACAGAGCATTAGGCGGTGGTAAGCACCGCGGTGGAGGACGAGGTGGGGGTGGTGGACGTGGAAGAGGAGGAGGGGGTCGGTGGAGAAACTGATGTTTTTCCCAACACTTTTCAGTCTCACCACTTTGGACTCAATTTTCCTGCGAAGAATTTACCACAAAATCCCCACCAATTCAAGCTTCTTGGCCATGAGTTCAATGAAAAAAGTAGAGGAATAATTTTTTTATGCCCATAATTTATGCTTTTCTTAGCCTGCTTGATGTATGAACTCAAAATTATGATACAATTTTCTGACGATAATTGTTAAAGGATGAGTTTTTATGTTAATTTTAGAGAGAATTAATGTCCGAATATTATCCAAGACTCTTAAATTTTGTTGGTTT

Coding sequence (CDS)

ATGGCGGAAGAGACAATCCCTCAACTCGATCCGGAGGTAACCAACTCAGGCACTGAAGATATGGAATTGGAAACTACGGAACCGAAAGCTTCAACTGATGCTACTGAAGCAAATGAGGCCGCTCCAGTAATTAATGGCGATGCTACTTCGAAGCGAGAGAGGGAGGAAAGCGCCGATGATGGCGTCGCCGATGGGAAGAAGCAGAAGATAGAGAAATCTGTTGAGGAGGAAAGGCTTGAGAAGTTGGGTGGAGACGGTAAGTGTGAGGAGGAACCTGTTCCTGTGAGCTTAGGCCCCAAGAGCTTTCGATCATCGGTGGAATTGTTTGATTACTTCTATAAGATCCTTCACCACTGGCCTGTCAACTTGAACGTGAACCAGTACGAGCAAATGGTGCTGGTAGATTTACTTAAGAAGGGACATCTAGAACCAGAGAAAAAGATTGGCTGTGGTATTCACTCTTTTCAAATTCGTTTCCATCCTGAGTGGAAAAGCAAGTGCTTCTTCCTCATCCGGGAGGATGAGTCTGCTGATGACTTCAGCTTCAGGAAGTGTGTTGATCATATCCTTCCTCTGCCTGAAAACTTCAAAGGAAAATTTGATGCAAACAGAGCATTAGGCGGTGGTAAGCACCGCGGTGGAGGACGAGGTGGGGGTGGTGGACGTGGAAGAGGAGGAGGGGGTCGGTGGAGAAACTGA

Protein sequence

MAEETIPQLDPEVTNSGTEDMELETTEPKASTDATEANEAAPVINGDATSKREREESADDGVADGKKQKIEKSVEEERLEKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYFYKILHHWPVNLNVNQYEQMVLVDLLKKGHLEPEKKIGCGIHSFQIRFHPEWKSKCFFLIREDESADDFSFRKCVDHILPLPENFKGKFDANRALGGGKHRGGGRGGGGGRGRGGGGRWRN*

Homology

[Close]

BLAST of CSPI01G02270 vs. ExPASy Swiss-Prot
Match: Q8L557 (Protein EMBRYO DEFECTIVE 514 OS=Arabidopsis thaliana OX=3702 GN=EMB514 PE=1 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 2.1e-46
Identity = 117/229 (51.09%), Postives = 141/229 (61.57%), Query Frame = 0


Query: 3   EETIPQLDPEVTNSGTEDMELETTEPKASTDATEANEAAPVINGDATSKREREESADDGV 62
           +E +  L  EV      DME+ET  PKA T             GD   +RE  E  ++G 
Sbjct: 5   QEIVDSLSAEVNPDQKVDMEVETATPKAET-------------GDEKREREETEEEENG- 64

Query: 63  ADGKKQKIEKSVEEERLEKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYFYKILHHWPVN 122
            + KKQK+ +                EE+  PV LGPK F +SV +FDYF K LH WP +
Sbjct: 65  GESKKQKVGE----------------EEKSGPVKLGPKEFVTSVAMFDYFVKFLHFWPTD 124

Query: 123 LNVNQYEQMVLVDLLKKGHLEPEKKIGCGIHSFQIRFHPEWKSKCFFLIREDESADDFSF 182
           L+VN+YE MVL+DL+KKGH EPEKKIG GI +FQ+R HP WKS+CFFL+RED++ADDFSF
Sbjct: 125 LDVNKYEHMVLLDLIKKGHSEPEKKIGGGIKTFQVRTHPMWKSRCFFLVREDDTADDFSF 184

Query: 183 RKCVDHILPLPENFKGKFDANRALGGGKHRGGGRGGGGGRGRGGGGRWR 232
           RKCVD ILPLPEN K         GGG  RGGG G  GGRG G GGR+R
Sbjct: 185 RKCVDQILPLPENMKTPGANGNGHGGG--RGGGGGRRGGRGGGRGGRFR 201

[Close]

BLAST of CSPI01G02270 vs. ExPASy Swiss-Prot
Match: Q9C642 (Protein DCL homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DCL PE=2 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 2.1e-06
Identity = 46/164 (28.05%), Postives = 73/164 (44.51%), Query Frame = 0


Query: 46  GDATSKRERE---ESADDGVADGKKQKI-------------EKSVEEERLEKLGGDGKCE 105
           GD+TS R R     +  DG   G  +               E S EEE  E+   +G   
Sbjct: 38  GDSTSLRPRVRALRTESDGAKIGNSESYGSELLRRPRIASEESSEEEEEEEEENSEG--- 97

Query: 106 EEPVPVSLGPKSFRSSVELFDYFYKILH--HWPVNLNVNQYEQMVLVDLLKKGHLEPEKK 165
                V    K    +V L  +   ILH   +     ++   +  ++++L   H E EKK
Sbjct: 98  --DEFVDWEDKILEVTVPLVGFVRMILHSGKYANRDRLSPEHERTIIEMLLPYHPECEKK 157

Query: 166 IGCGIHSFQIRFHPEWK-SKCFFLIREDESADDFSFRKCVDHIL 191
           IGCGI    +  HP+++ S+C F++R+D    DFS+ KC+  ++
Sbjct: 158 IGCGIDYIMVGHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLI 196

[Close]

BLAST of CSPI01G02270 vs. ExPASy Swiss-Prot
Match: Q42463 (Protein DCL, chloroplastic OS=Solanum lycopersicum OX=4081 GN=DCL PE=2 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.1e-05
Identity = 32/128 (25.00%), Postives = 64/128 (50.00%), Query Frame = 0


Query: 66  KKQKIEKSVEEERLEKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYFYKILH--HWPVNL 125
           + +++ K   ++ + K  GDG+       V    +    +V L  +   ILH   + +  
Sbjct: 79  ESEELVKEESDDEVGKKSGDGEGW-----VDWEDQILEDTVPLVGFVRMILHSGKYAIGD 138

Query: 126 NVNQYEQMVLVDLLKKGHLEPEKKIGCGIHSFQIRFHPEWK-SKCFFLIREDESADDFSF 185
            ++   Q  ++  L   H E +KKIG G+    + +HP+++ S+C F++R+D    DFS+
Sbjct: 139 RLSPDHQRTILQRLLPYHPECDKKIGPGVDYITVGYHPDFENSRCLFIVRKDGETVDFSY 198

Query: 186 RKCVDHIL 191
            KC+  ++
Sbjct: 199 WKCIKGLI 201

[Close]

BLAST of CSPI01G02270 vs. ExPASy Swiss-Prot
Match: Q9LQ02 (DNA-directed RNA polymerase IV subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPD1 PE=1 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 2.6e-04
Identity = 39/124 (31.45%), Postives = 62/124 (50.00%), Query Frame = 0


Query: 93   VPVSLGPKSFR-SSVELFDYFYK-ILHHWPVNLNVNQYEQMVLVDLLKKGHLEPEKKIGC 152
            +P+SL    F   ++EL     K ILH + +N  +N+ ++  LV ++ + H    +KIG 
Sbjct: 1325 IPMSLLRTIFTWKNIELLSQSLKRILHSYEINELLNERDE-GLVKMVLQLHPNSVEKIGP 1384

Query: 153  GIHSFQIRFHPEWKSKCFFLIREDESADDFSFRKCV----DHILPLPENF-KGKFDANRA 210
            G+   ++       S CF ++R D + +DFS+ KCV      I P   NF K K+  N  
Sbjct: 1385 GVKGIRVAKSKHGDSCCFEVVRIDGTFEDFSYHKCVLGATKIIAPKKMNFYKSKYLKNGT 1444

[Close]

BLAST of CSPI01G02270 vs. ExPASy TrEMBL
Match: A0A0A0LRN9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G009730 PE=4 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 3.8e-123
Identity = 231/232 (99.57%), Postives = 232/232 (100.00%), Query Frame = 0


Query: 1   MAEETIPQLDPEVTNSGTEDMELETTEPKASTDATEANEAAPVINGDATSKREREESADD 60
           MAEETIPQLDPEVTNSGTEDMELETTEPKASTDA+EANEAAPVINGDATSKREREESADD
Sbjct: 1   MAEETIPQLDPEVTNSGTEDMELETTEPKASTDASEANEAAPVINGDATSKREREESADD 60

Query: 61  GVADGKKQKIEKSVEEERLEKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYFYKILHHWP 120
           GVADGKKQKIEKSVEEERLEKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYFYKILHHWP
Sbjct: 61  GVADGKKQKIEKSVEEERLEKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYFYKILHHWP 120

Query: 121 VNLNVNQYEQMVLVDLLKKGHLEPEKKIGCGIHSFQIRFHPEWKSKCFFLIREDESADDF 180
           VNLNVNQYEQMVLVDLLKKGHLEPEKKIGCGIHSFQIRFHPEWKSKCFFLIREDESADDF
Sbjct: 121 VNLNVNQYEQMVLVDLLKKGHLEPEKKIGCGIHSFQIRFHPEWKSKCFFLIREDESADDF 180

Query: 181 SFRKCVDHILPLPENFKGKFDANRALGGGKHRGGGRGGGGGRGRGGGGRWRN 233
           SFRKCVDHILPLPENFKGKFDANRALGGGKHRGGGRGGGGGRGRGGGGRWRN
Sbjct: 181 SFRKCVDHILPLPENFKGKFDANRALGGGKHRGGGRGGGGGRGRGGGGRWRN 232

[Close]

BLAST of CSPI01G02270 vs. ExPASy TrEMBL
Match: A0A5A7UQ03 (Protein DCL OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002450 PE=4 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 1.1e-114
Identity = 217/232 (93.53%), Postives = 223/232 (96.12%), Query Frame = 0


Query: 1   MAEETIPQLDPEVTNSGTEDMELETTEPKASTDATEANEAAPVINGDATSKREREESADD 60
           MAEETIPQLDPEVTNSGTEDMELETT PK S+DA+EA EAAPVINGDA SKREREESADD
Sbjct: 1   MAEETIPQLDPEVTNSGTEDMELETTVPKGSSDASEATEAAPVINGDANSKREREESADD 60

Query: 61  GVADGKKQKIEKSVEEERLEKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYFYKILHHWP 120
           GV DGKKQKIEKSVEEER+EKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYFYKILH+WP
Sbjct: 61  GVGDGKKQKIEKSVEEERVEKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYFYKILHNWP 120

Query: 121 VNLNVNQYEQMVLVDLLKKGHLEPEKKIGCGIHSFQIRFHPEWKSKCFFLIREDESADDF 180
           +NLNVNQYEQMVLVDLLKKGHLEPEKKIGCGI +FQIRFHP WKSKCFFLIREDESADDF
Sbjct: 121 INLNVNQYEQMVLVDLLKKGHLEPEKKIGCGIQAFQIRFHPVWKSKCFFLIREDESADDF 180

Query: 181 SFRKCVDHILPLPENFKGKFDANRALGGGKHRGGGRGGGGGRGRGGGGRWRN 233
           SFRKCVDHILPLPENFK K DANRALGGGKHRGGGRGGGGGRGRGGGGRWRN
Sbjct: 181 SFRKCVDHILPLPENFKAKSDANRALGGGKHRGGGRGGGGGRGRGGGGRWRN 232

[Close]

BLAST of CSPI01G02270 vs. ExPASy TrEMBL
Match: A0A1S3BWT9 (protein DCL, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493992 PE=4 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 1.1e-114
Identity = 217/232 (93.53%), Postives = 223/232 (96.12%), Query Frame = 0


Query: 1   MAEETIPQLDPEVTNSGTEDMELETTEPKASTDATEANEAAPVINGDATSKREREESADD 60
           MAEETIPQLDPEVTNSGTEDMELETT PK S+DA+EA EAAPVINGDA SKREREESADD
Sbjct: 1   MAEETIPQLDPEVTNSGTEDMELETTVPKGSSDASEATEAAPVINGDANSKREREESADD 60

Query: 61  GVADGKKQKIEKSVEEERLEKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYFYKILHHWP 120
           GV DGKKQKIEKSVEEER+EKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYFYKILH+WP
Sbjct: 61  GVGDGKKQKIEKSVEEERVEKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYFYKILHNWP 120

Query: 121 VNLNVNQYEQMVLVDLLKKGHLEPEKKIGCGIHSFQIRFHPEWKSKCFFLIREDESADDF 180
           +NLNVNQYEQMVLVDLLKKGHLEPEKKIGCGI +FQIRFHP WKSKCFFLIREDESADDF
Sbjct: 121 INLNVNQYEQMVLVDLLKKGHLEPEKKIGCGIQAFQIRFHPVWKSKCFFLIREDESADDF 180

Query: 181 SFRKCVDHILPLPENFKGKFDANRALGGGKHRGGGRGGGGGRGRGGGGRWRN 233
           SFRKCVDHILPLPENFK K DANRALGGGKHRGGGRGGGGGRGRGGGGRWRN
Sbjct: 181 SFRKCVDHILPLPENFKAKSDANRALGGGKHRGGGRGGGGGRGRGGGGRWRN 232

[Close]

BLAST of CSPI01G02270 vs. ExPASy TrEMBL
Match: A0A6J1C0K4 (protein EMBRYO DEFECTIVE 514 OS=Momordica charantia OX=3673 GN=LOC111007244 PE=4 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 1.1e-85
Identity = 178/237 (75.11%), Postives = 199/237 (83.97%), Query Frame = 0


Query: 1   MAEETIPQL---DPEVTNSGTEDMELETT----EPKASTDATEANEAAPVINGDATSKRE 60
           MAEET PQL   DPEVTNS TEDMELET+    EPK     +EANEAAP  NGDA SKRE
Sbjct: 1   MAEETTPQLAENDPEVTNSATEDMELETSEPGAEPKGLNGDSEANEAAPETNGDANSKRE 60

Query: 61  R-EESADDGVADGKKQKIEKSVEEERLEKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYF 120
           R EE+AD+ + + KK K+EKSVEEER+EK+ GDGK +E+  PVSLGPKSF SSVE+FDYF
Sbjct: 61  REEETADENIGEAKKPKVEKSVEEERMEKVEGDGKGDEKSGPVSLGPKSFGSSVEMFDYF 120

Query: 121 YKILHHWPVNLNVNQYEQMVLVDLLKKGHLEPEKKIGCGIHSFQIRFHPEWKSKCFFLIR 180
           YK LH+WP NLN+N+YEQMVL++LLKKGH+EPEKKIGCGIH+FQIR HP +KSKC+FLIR
Sbjct: 121 YKFLHYWPANLNLNEYEQMVLLELLKKGHVEPEKKIGCGIHAFQIRNHPTFKSKCYFLIR 180

Query: 181 EDESADDFSFRKCVDHILPLPENFKGKFDANRALGGGKHRGGGRGGGGGRGRGGGGR 230
           EDES DDFSFRKCVDHILPLPEN K K DANRALGG KHRGGGRGGGGGRGRGG  R
Sbjct: 181 EDESVDDFSFRKCVDHILPLPENLKVKSDANRALGGCKHRGGGRGGGGGRGRGGRSR 237

[Close]

BLAST of CSPI01G02270 vs. ExPASy TrEMBL
Match: A0A6J1F569 (protein EMBRYO DEFECTIVE 514-like OS=Cucurbita moschata OX=3662 GN=LOC111440949 PE=4 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 1.8e-85
Identity = 177/236 (75.00%), Postives = 191/236 (80.93%), Query Frame = 0


Query: 1   MAEETIPQL---DPEVTNSGTEDMELETTEPKASTDATEANEAAPVINGDATSKRER-EE 60
           MAEETIPQL    PEVTNS  EDMELE+TEPK S   ++ANEAAPV NGDA SKRER EE
Sbjct: 1   MAEETIPQLAENGPEVTNSAAEDMELESTEPKVSGGDSDANEAAPVTNGDANSKREREEE 60

Query: 61  SADDGVADGKKQKIEKSVEEERLEKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYFYKIL 120
            ADD   + KK K+EKSVEEERLEK+  DGK EEE  PVSLGPKSF SSVELFDYFY  L
Sbjct: 61  GADDHAGEAKKPKVEKSVEEERLEKVERDGKGEEEHGPVSLGPKSFGSSVELFDYFYNFL 120

Query: 121 HHWPVNLNVNQYEQMVLVDLLKKGHLEPEKKIGCGIHSFQIRFHPEWKSKCFFLIREDES 180
           H+WP NLNVNQYE MVL+DL+KKGH+EP+KKIGCG+ + QIR+HP WKSKCFFLIRED S
Sbjct: 121 HYWPANLNVNQYELMVLLDLIKKGHVEPDKKIGCGVRACQIRYHPMWKSKCFFLIREDGS 180

Query: 181 ADDFSFRKCVDHILPLPENFKGKFDANRALGGGKHRGGGRGGGGGRGRGGGGRWRN 233
           ADDFSFRKCVDHILPLPEN K   DANRALGGGKHRGG        GRGGGGRWRN
Sbjct: 181 ADDFSFRKCVDHILPLPENLKTISDANRALGGGKHRGG--------GRGGGGRWRN 228

[Close]

BLAST of CSPI01G02270 vs. NCBI nr
Match: XP_011660085.1 (protein EMBRYO DEFECTIVE 514 [Cucumis sativus] >KGN63669.1 hypothetical protein Csa_014307 [Cucumis sativus])

HSP 1 Score: 450.7 bits (1158), Expect = 7.8e-123
Identity = 231/232 (99.57%), Postives = 232/232 (100.00%), Query Frame = 0


Query: 1   MAEETIPQLDPEVTNSGTEDMELETTEPKASTDATEANEAAPVINGDATSKREREESADD 60
           MAEETIPQLDPEVTNSGTEDMELETTEPKASTDA+EANEAAPVINGDATSKREREESADD
Sbjct: 1   MAEETIPQLDPEVTNSGTEDMELETTEPKASTDASEANEAAPVINGDATSKREREESADD 60

Query: 61  GVADGKKQKIEKSVEEERLEKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYFYKILHHWP 120
           GVADGKKQKIEKSVEEERLEKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYFYKILHHWP
Sbjct: 61  GVADGKKQKIEKSVEEERLEKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYFYKILHHWP 120

Query: 121 VNLNVNQYEQMVLVDLLKKGHLEPEKKIGCGIHSFQIRFHPEWKSKCFFLIREDESADDF 180
           VNLNVNQYEQMVLVDLLKKGHLEPEKKIGCGIHSFQIRFHPEWKSKCFFLIREDESADDF
Sbjct: 121 VNLNVNQYEQMVLVDLLKKGHLEPEKKIGCGIHSFQIRFHPEWKSKCFFLIREDESADDF 180

Query: 181 SFRKCVDHILPLPENFKGKFDANRALGGGKHRGGGRGGGGGRGRGGGGRWRN 233
           SFRKCVDHILPLPENFKGKFDANRALGGGKHRGGGRGGGGGRGRGGGGRWRN
Sbjct: 181 SFRKCVDHILPLPENFKGKFDANRALGGGKHRGGGRGGGGGRGRGGGGRWRN 232

[Close]

BLAST of CSPI01G02270 vs. NCBI nr
Match: XP_008453205.1 (PREDICTED: protein DCL, chloroplastic [Cucumis melo] >KAA0057942.1 protein DCL [Cucumis melo var. makuwa])

HSP 1 Score: 422.5 bits (1085), Expect = 2.3e-114
Identity = 217/232 (93.53%), Postives = 223/232 (96.12%), Query Frame = 0


Query: 1   MAEETIPQLDPEVTNSGTEDMELETTEPKASTDATEANEAAPVINGDATSKREREESADD 60
           MAEETIPQLDPEVTNSGTEDMELETT PK S+DA+EA EAAPVINGDA SKREREESADD
Sbjct: 1   MAEETIPQLDPEVTNSGTEDMELETTVPKGSSDASEATEAAPVINGDANSKREREESADD 60

Query: 61  GVADGKKQKIEKSVEEERLEKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYFYKILHHWP 120
           GV DGKKQKIEKSVEEER+EKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYFYKILH+WP
Sbjct: 61  GVGDGKKQKIEKSVEEERVEKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYFYKILHNWP 120

Query: 121 VNLNVNQYEQMVLVDLLKKGHLEPEKKIGCGIHSFQIRFHPEWKSKCFFLIREDESADDF 180
           +NLNVNQYEQMVLVDLLKKGHLEPEKKIGCGI +FQIRFHP WKSKCFFLIREDESADDF
Sbjct: 121 INLNVNQYEQMVLVDLLKKGHLEPEKKIGCGIQAFQIRFHPVWKSKCFFLIREDESADDF 180

Query: 181 SFRKCVDHILPLPENFKGKFDANRALGGGKHRGGGRGGGGGRGRGGGGRWRN 233
           SFRKCVDHILPLPENFK K DANRALGGGKHRGGGRGGGGGRGRGGGGRWRN
Sbjct: 181 SFRKCVDHILPLPENFKAKSDANRALGGGKHRGGGRGGGGGRGRGGGGRWRN 232

[Close]

BLAST of CSPI01G02270 vs. NCBI nr
Match: XP_038880608.1 (protein EMBRYO DEFECTIVE 514 [Benincasa hispida])

HSP 1 Score: 383.3 bits (983), Expect = 1.5e-102
Identity = 208/240 (86.67%), Postives = 217/240 (90.42%), Query Frame = 0


Query: 1   MAEETIPQL---DPEVTNSGTEDMELETTEP----KASTDATEANEAAPVINGDATSKRE 60
           MAEETIPQL   DP VTNSGTEDMELETTEP    K ST+ +EA EA PV NGD+TSKRE
Sbjct: 1   MAEETIPQLVENDPAVTNSGTEDMELETTEPETGAKVSTETSEATEAVPVTNGDSTSKRE 60

Query: 61  R-EESADDGVADGKKQKIEKSVEEERLEKLGGDGKC-EEEPVPVSLGPKSFRSSVELFDY 120
           R EESADDGV DGKKQKIEKSVEEER EK+GGDGK  EEEPV VSLGPKSF+SS+ELFDY
Sbjct: 61  REEESADDGVGDGKKQKIEKSVEEERFEKVGGDGKGEEEEPVSVSLGPKSFQSSMELFDY 120

Query: 121 FYKILHHWPVNLNVNQYEQMVLVDLLKKGHLEPEKKIGCGIHSFQIRFHPEWKSKCFFLI 180
           FYKILHHWPVNLNVNQYEQM LVDLLKKGH+EPEKKIGCGIH+FQIR+HP WKSKCFFLI
Sbjct: 121 FYKILHHWPVNLNVNQYEQMALVDLLKKGHVEPEKKIGCGIHAFQIRYHPVWKSKCFFLI 180

Query: 181 REDESADDFSFRKCVDHILPLPENFKGKFDANRALGGGKHRGGGRGGGGGRGRGGGGRWR 232
           REDESADDFSFRKCVDHILPLPENFKGK DANRALGGGKHRGGGRGGGGGRGR GGGRWR
Sbjct: 181 REDESADDFSFRKCVDHILPLPENFKGKSDANRALGGGKHRGGGRGGGGGRGR-GGGRWR 239

[Close]

BLAST of CSPI01G02270 vs. NCBI nr
Match: XP_022135230.1 (protein EMBRYO DEFECTIVE 514 [Momordica charantia])

HSP 1 Score: 326.2 bits (835), Expect = 2.2e-85
Identity = 178/237 (75.11%), Postives = 199/237 (83.97%), Query Frame = 0


Query: 1   MAEETIPQL---DPEVTNSGTEDMELETT----EPKASTDATEANEAAPVINGDATSKRE 60
           MAEET PQL   DPEVTNS TEDMELET+    EPK     +EANEAAP  NGDA SKRE
Sbjct: 1   MAEETTPQLAENDPEVTNSATEDMELETSEPGAEPKGLNGDSEANEAAPETNGDANSKRE 60

Query: 61  R-EESADDGVADGKKQKIEKSVEEERLEKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYF 120
           R EE+AD+ + + KK K+EKSVEEER+EK+ GDGK +E+  PVSLGPKSF SSVE+FDYF
Sbjct: 61  REEETADENIGEAKKPKVEKSVEEERMEKVEGDGKGDEKSGPVSLGPKSFGSSVEMFDYF 120

Query: 121 YKILHHWPVNLNVNQYEQMVLVDLLKKGHLEPEKKIGCGIHSFQIRFHPEWKSKCFFLIR 180
           YK LH+WP NLN+N+YEQMVL++LLKKGH+EPEKKIGCGIH+FQIR HP +KSKC+FLIR
Sbjct: 121 YKFLHYWPANLNLNEYEQMVLLELLKKGHVEPEKKIGCGIHAFQIRNHPTFKSKCYFLIR 180

Query: 181 EDESADDFSFRKCVDHILPLPENFKGKFDANRALGGGKHRGGGRGGGGGRGRGGGGR 230
           EDES DDFSFRKCVDHILPLPEN K K DANRALGG KHRGGGRGGGGGRGRGG  R
Sbjct: 181 EDESVDDFSFRKCVDHILPLPENLKVKSDANRALGGCKHRGGGRGGGGGRGRGGRSR 237

[Close]

BLAST of CSPI01G02270 vs. NCBI nr
Match: XP_022933555.1 (protein EMBRYO DEFECTIVE 514-like [Cucurbita moschata])

HSP 1 Score: 325.5 bits (833), Expect = 3.8e-85
Identity = 177/236 (75.00%), Postives = 191/236 (80.93%), Query Frame = 0


Query: 1   MAEETIPQL---DPEVTNSGTEDMELETTEPKASTDATEANEAAPVINGDATSKRER-EE 60
           MAEETIPQL    PEVTNS  EDMELE+TEPK S   ++ANEAAPV NGDA SKRER EE
Sbjct: 1   MAEETIPQLAENGPEVTNSAAEDMELESTEPKVSGGDSDANEAAPVTNGDANSKREREEE 60

Query: 61  SADDGVADGKKQKIEKSVEEERLEKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYFYKIL 120
            ADD   + KK K+EKSVEEERLEK+  DGK EEE  PVSLGPKSF SSVELFDYFY  L
Sbjct: 61  GADDHAGEAKKPKVEKSVEEERLEKVERDGKGEEEHGPVSLGPKSFGSSVELFDYFYNFL 120

Query: 121 HHWPVNLNVNQYEQMVLVDLLKKGHLEPEKKIGCGIHSFQIRFHPEWKSKCFFLIREDES 180
           H+WP NLNVNQYE MVL+DL+KKGH+EP+KKIGCG+ + QIR+HP WKSKCFFLIRED S
Sbjct: 121 HYWPANLNVNQYELMVLLDLIKKGHVEPDKKIGCGVRACQIRYHPMWKSKCFFLIREDGS 180

Query: 181 ADDFSFRKCVDHILPLPENFKGKFDANRALGGGKHRGGGRGGGGGRGRGGGGRWRN 233
           ADDFSFRKCVDHILPLPEN K   DANRALGGGKHRGG        GRGGGGRWRN
Sbjct: 181 ADDFSFRKCVDHILPLPENLKTISDANRALGGGKHRGG--------GRGGGGRWRN 228

[Close]

BLAST of CSPI01G02270 vs. TAIR 10
Match: AT5G62440.1 (Protein of unknown function (DUF3223) )

HSP 1 Score: 187.2 bits (474), Expect = 1.5e-47
Identity = 117/229 (51.09%), Postives = 141/229 (61.57%), Query Frame = 0


Query: 3   EETIPQLDPEVTNSGTEDMELETTEPKASTDATEANEAAPVINGDATSKREREESADDGV 62
           +E +  L  EV      DME+ET  PKA T             GD   +RE  E  ++G 
Sbjct: 5   QEIVDSLSAEVNPDQKVDMEVETATPKAET-------------GDEKREREETEEEENG- 64

Query: 63  ADGKKQKIEKSVEEERLEKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYFYKILHHWPVN 122
            + KKQK+ +                EE+  PV LGPK F +SV +FDYF K LH WP +
Sbjct: 65  GESKKQKVGE----------------EEKSGPVKLGPKEFVTSVAMFDYFVKFLHFWPTD 124

Query: 123 LNVNQYEQMVLVDLLKKGHLEPEKKIGCGIHSFQIRFHPEWKSKCFFLIREDESADDFSF 182
           L+VN+YE MVL+DL+KKGH EPEKKIG GI +FQ+R HP WKS+CFFL+RED++ADDFSF
Sbjct: 125 LDVNKYEHMVLLDLIKKGHSEPEKKIGGGIKTFQVRTHPMWKSRCFFLVREDDTADDFSF 184

Query: 183 RKCVDHILPLPENFKGKFDANRALGGGKHRGGGRGGGGGRGRGGGGRWR 232
           RKCVD ILPLPEN K         GGG  RGGG G  GGRG G GGR+R
Sbjct: 185 RKCVDQILPLPENMKTPGANGNGHGGG--RGGGGGRRGGRGGGRGGRFR 201

[Close]

BLAST of CSPI01G02270 vs. TAIR 10
Match: AT1G45230.1 (Protein of unknown function (DUF3223) )

HSP 1 Score: 54.3 bits (129), Expect = 1.5e-07
Identity = 46/164 (28.05%), Postives = 73/164 (44.51%), Query Frame = 0


Query: 46  GDATSKRERE---ESADDGVADGKKQKI-------------EKSVEEERLEKLGGDGKCE 105
           GD+TS R R     +  DG   G  +               E S EEE  E+   +G   
Sbjct: 38  GDSTSLRPRVRALRTESDGAKIGNSESYGSELLRRPRIASEESSEEEEEEEEENSEG--- 97

Query: 106 EEPVPVSLGPKSFRSSVELFDYFYKILH--HWPVNLNVNQYEQMVLVDLLKKGHLEPEKK 165
                V    K    +V L  +   ILH   +     ++   +  ++++L   H E EKK
Sbjct: 98  --DEFVDWEDKILEVTVPLVGFVRMILHSGKYANRDRLSPEHERTIIEMLLPYHPECEKK 157

Query: 166 IGCGIHSFQIRFHPEWK-SKCFFLIREDESADDFSFRKCVDHIL 191
           IGCGI    +  HP+++ S+C F++R+D    DFS+ KC+  ++
Sbjct: 158 IGCGIDYIMVGHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLI 196

[Close]

BLAST of CSPI01G02270 vs. TAIR 10
Match: AT1G45230.2 (Protein of unknown function (DUF3223) )

HSP 1 Score: 53.9 bits (128), Expect = 2.0e-07
Identity = 46/164 (28.05%), Postives = 73/164 (44.51%), Query Frame = 0


Query: 46  GDATSKRERE---ESADDGVADGKKQKI-------------EKSVEEERLEKLGGDGKCE 105
           GD+TS R R     +  DG   G  +               E S EEE  E+   +G   
Sbjct: 38  GDSTSLRPRVRALRTESDGAKIGNSESYGSELLRRPRIASEESSEEEEEEEEENSEG--- 97

Query: 106 EEPVPVSLGPKSFRSSVELFDYFYKILH--HWPVNLNVNQYEQMVLVDLLKKGHLEPEKK 165
                V    K    +V L  +   ILH   +     ++   +  ++++L   H E EKK
Sbjct: 98  --DEFVDWEDKILEVTVPLVGFVRMILHSGKYANRDRLSPEHERTIIEMLLPYHPECEKK 157

Query: 166 IGCGIHSFQIRFHPEWK-SKCFFLIREDESADDFSFRKCVDHIL 191
           IGCGI    +  HP+++ S+C F++R+D    DFS+ KC+  ++
Sbjct: 158 IGCGIDYIMVWHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLI 196

[Close]

BLAST of CSPI01G02270 vs. TAIR 10
Match: AT1G63020.1 (nuclear RNA polymerase D1A )

HSP 1 Score: 47.4 bits (111), Expect = 1.9e-05
Identity = 39/124 (31.45%), Postives = 62/124 (50.00%), Query Frame = 0


Query: 93   VPVSLGPKSFR-SSVELFDYFYK-ILHHWPVNLNVNQYEQMVLVDLLKKGHLEPEKKIGC 152
            +P+SL    F   ++EL     K ILH + +N  +N+ ++  LV ++ + H    +KIG 
Sbjct: 1325 IPMSLLRTIFTWKNIELLSQSLKRILHSYEINELLNERDE-GLVKMVLQLHPNSVEKIGP 1384

Query: 153  GIHSFQIRFHPEWKSKCFFLIREDESADDFSFRKCV----DHILPLPENF-KGKFDANRA 210
            G+   ++       S CF ++R D + +DFS+ KCV      I P   NF K K+  N  
Sbjct: 1385 GVKGIRVAKSKHGDSCCFEVVRIDGTFEDFSYHKCVLGATKIIAPKKMNFYKSKYLKNGT 1444

[Close]

BLAST of CSPI01G02270 vs. TAIR 10
Match: AT1G63020.2 (nuclear RNA polymerase D1A )

HSP 1 Score: 47.4 bits (111), Expect = 1.9e-05
Identity = 39/124 (31.45%), Postives = 62/124 (50.00%), Query Frame = 0


Query: 93   VPVSLGPKSFR-SSVELFDYFYK-ILHHWPVNLNVNQYEQMVLVDLLKKGHLEPEKKIGC 152
            +P+SL    F   ++EL     K ILH + +N  +N+ ++  LV ++ + H    +KIG 
Sbjct: 1325 IPMSLLRTIFTWKNIELLSQSLKRILHSYEINELLNERDE-GLVKMVLQLHPNSVEKIGP 1384

Query: 153  GIHSFQIRFHPEWKSKCFFLIREDESADDFSFRKCV----DHILPLPENF-KGKFDANRA 210
            G+   ++       S CF ++R D + +DFS+ KCV      I P   NF K K+  N  
Sbjct: 1385 GVKGIRVAKSKHGDSCCFEVVRIDGTFEDFSYHKCVLGATKIIAPKKMNFYKSKYLKNGT 1444

The following BLAST results are available for this feature:

BLAST of CSPI01G02270 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L557	2.1e-46	51.09	Protein EMBRYO DEFECTIVE 514 OS=Arabidopsis thaliana OX=3702 GN=EMB514 PE=1 SV=1	[more]
Q9C642	2.1e-06	28.05	Protein DCL homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DCL PE=2 S...	[more]
Q42463	1.1e-05	25.00	Protein DCL, chloroplastic OS=Solanum lycopersicum OX=4081 GN=DCL PE=2 SV=1	[more]
Q9LQ02	2.6e-04	31.45	DNA-directed RNA polymerase IV subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPD...	[more]

BLAST of CSPI01G02270 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LRN9	3.8e-123	99.57	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G009730 PE=4 SV=1	[more]
A0A5A7UQ03	1.1e-114	93.53	Protein DCL OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002450 P...	[more]
A0A1S3BWT9	1.1e-114	93.53	protein DCL, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493992 PE=4 SV=1	[more]
A0A6J1C0K4	1.1e-85	75.11	protein EMBRYO DEFECTIVE 514 OS=Momordica charantia OX=3673 GN=LOC111007244 PE=4...	[more]
A0A6J1F569	1.8e-85	75.00	protein EMBRYO DEFECTIVE 514-like OS=Cucurbita moschata OX=3662 GN=LOC111440949 ...	[more]

BLAST of CSPI01G02270 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011660085.1	7.8e-123	99.57	protein EMBRYO DEFECTIVE 514 [Cucumis sativus] >KGN63669.1 hypothetical protein ...	[more]
XP_008453205.1	2.3e-114	93.53	PREDICTED: protein DCL, chloroplastic [Cucumis melo] >KAA0057942.1 protein DCL [...	[more]
XP_038880608.1	1.5e-102	86.67	protein EMBRYO DEFECTIVE 514 [Benincasa hispida]	[more]
XP_022135230.1	2.2e-85	75.11	protein EMBRYO DEFECTIVE 514 [Momordica charantia]	[more]
XP_022933555.1	3.8e-85	75.00	protein EMBRYO DEFECTIVE 514-like [Cucurbita moschata]	[more]

BLAST of CSPI01G02270 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G62440.1	1.5e-47	51.09	Protein of unknown function (DUF3223)	[more]
AT1G45230.1	1.5e-07	28.05	Protein of unknown function (DUF3223)	[more]
AT1G45230.2	2.0e-07	28.05	Protein of unknown function (DUF3223)	[more]
AT1G63020.1	1.9e-05	31.45	nuclear RNA polymerase D1A	[more]
AT1G63020.2	1.9e-05	31.45	nuclear RNA polymerase D1A	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF11523	DUF3223	coord: 114..186 e-value: 1.6E-25 score: 89.6
None	No IPR available	GENE3D	3.10.450.40		coord: 91..189 e-value: 5.4E-28 score: 99.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 207..232
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 48..88
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..95
None	No IPR available	PANTHER	PTHR33415:SF12	PROTEIN EMBRYO DEFECTIVE 514	coord: 13..220
IPR044673	Protein DCL-like	PANTHER	PTHR33415	FAMILY NOT NAMED	coord: 13..220

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G02270.1	CSPI01G02270.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017126	nucleologenesis
cellular_component	GO:0005634	nucleus