CSPI01G01600 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G01600
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Aminoacylase-1
Location	Chr1: 1066364 .. 1068470 (+)
RNA-Seq Expression	CSPI01G01600
Synteny	CSPI01G01600

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAGCCTCTAGAGTTAAGATGAATAGGGAAGAACAAACCAAATACACAAATATATATACATATACACAAAGTTTTATATGATTAATTTAGGGTATGATTCAAAGAATGTATAAAGTTTTATATAATTTAGCTAAGAGTTAAAAAAGAATTGGTGTGTTCTGTTGTATTTCGAGACTGTTCAAGATTGTTTTTTTCTTCCGACCCTAGGTTCGATCGGCGGCTATCTTTCCGATCGGCAGCTCTCCCACTTACTGAGACTACTTGACTATGTCGAACTTCCATCTTCACTATCGTCTCTTTTCAGTCACTCTTTATCTTCTTCTTCTTCTACTCTCATTTCCTCTCTCTTTCAGCCATTCACAGTACGTTGATGATGAACCCGATCAAGATTCTCCGGTCTCTCGCTTTCAACGCTACCTCAGAATCAAAACCGCTCATCCAGATCCCGATTACGCCTCAGCTGTCGCCTTCCTCAGATCTCAAGCACAAGAAATCGGTCTCCATACGCAGATACTCGAGTTCGTTACAGGTAAGCCTCTTCTTCTTGTGACCTGGTATGGATCAGACCCTTCTTTGCCTTCGATTTTACTCAACTCTCATATGGACTCTGTCCCCGCCGAGCCTTCTAAGTGGGTTCATCCTCCATTCTCCGCTGTCCGAACCTCTGATGGTAAAATCTTCGCCCGTGGTTCTCAAGATGACAAGTGTATCGCCATCCAGTATTTGGAAGCCATTCGGAATCTCAGAAATCGGGATTTCGTCCCTGTTCGTACGATTCATATCTCGTATGTACCGGATGAGGAGATTGGGGGTTCCGATGGAGCTGCGAAGTTCGTTCAGTCGAAGGAGTTCAAGGAGTTGAATGTGGGGTTTATGATGGATGAAGGACAAGCTTCACCGGGAGATGAGTTTAGGGTTTTTTACGCCGATAGATCGCCATGGTCGTTGATAATCAAGGCTAAAGGAAGCCCTGGCCATGGATCTAGAATGTACGATAACAGCGCAATGGAGAATTTGATGAAGAGCGTTGAGATTATGACTAGATTCAGAGAGAGCCAATTTGAAATAGTTAAAGCAGGTGAAGCTGCTAATTCAGAAGTCATCTCTGTGAATCCTGTTTTTGTCAATGCCGGAACTCCTTCCCCCACAGGATTTGTGATGAACATGCAACCGTCAGAGGCGGAGGCTGGGTTTGATCTCAGATTACCGCCGACCGCCGACCCAGATGCTATGAGAAGAAGAATAGCTGAGGAATGGGCTCCGGCACGGCGGAACATGACTTATCAGATACTTGAGAAAGGGCCCATAAGAGATTTCTTGGGAAGTCCATTAATGACGATGACAAATGCTTCAAATCCATGGTGGGCTGTTTTCAAGGCAGCCATTTCCGACGCTGGAGGGAAGCTTTCTAAGCCCGAAATCTTGGCTACAACTACGGATGCTCGATTCATGAGACAGATGAGGATTCCTGTTCTTGGTTTCTCTCCAATGATTAACACTCCAATCTTATTGCATGATCACAATGAGTTCTTGAGTGATTCTGTGTTCATAAGGGGAATCAAGGTTTATGAATCCTTAATCAGTGCTTTGAGTTCCTTCCAAGAGGATGTTTCTTCCCAATAATAAGCATTGCTCATCCTTCAGAATTCTCATGGGAATCAACACTGTAAAGTATCCATTCAGAAGGACAATCTCAAAGGCATAGCTTTATGCAAAAGTAAGCTTTCTTCCCAACCCGACCCAATTCACCGTTCGTGTCTATTTGGTCTTTAAACTTTAAATGGTTGTTTAGAATGCTTTTCATTTCATGGGTCTTCGGATACTTTCACGGAAAAAGAGTCGTATAAATGATAAATCTCAATGATATATGAAACTTTGTAGCATATTTTCATTGAGTTTGAGTTTATATTGTTGAATAAGACTGTTATGGTTGAGGAACTTAAATACTCTTCCACCCATCATTGTTATGGAGAATAAAACCTTGAAGTTTCTTTTGATGGAGTTTGTTAAATAGCTTTGTGGTTAAGGAACAACTCATTCATTACAAGAGTTGTTCAAAGTTACCTTTAAAATTGTATTGATAAACCAAACAAAATGAAAGGAGGCC

mRNA sequence

CAAGCCTCTAGAGTTAAGATGAATAGGGAAGAACAAACCAAATACACAAATATATATACATATACACAAAGTTTTATATGATTAATTTAGGGTATGATTCAAAGAATGTATAAAGTTTTATATAATTTAGCTAAGAGTTAAAAAAGAATTGGTGTGTTCTGTTGTATTTCGAGACTGTTCAAGATTGTTTTTTTCTTCCGACCCTAGGTTCGATCGGCGGCTATCTTTCCGATCGGCAGCTCTCCCACTTACTGAGACTACTTGACTATGTCGAACTTCCATCTTCACTATCGTCTCTTTTCAGTCACTCTTTATCTTCTTCTTCTTCTACTCTCATTTCCTCTCTCTTTCAGCCATTCACAGTACGTTGATGATGAACCCGATCAAGATTCTCCGGTCTCTCGCTTTCAACGCTACCTCAGAATCAAAACCGCTCATCCAGATCCCGATTACGCCTCAGCTGTCGCCTTCCTCAGATCTCAAGCACAAGAAATCGGTCTCCATACGCAGATACTCGAGTTCGTTACAGGTAAGCCTCTTCTTCTTGTGACCTGGTATGGATCAGACCCTTCTTTGCCTTCGATTTTACTCAACTCTCATATGGACTCTGTCCCCGCCGAGCCTTCTAAGTGGGTTCATCCTCCATTCTCCGCTGTCCGAACCTCTGATGGTAAAATCTTCGCCCGTGGTTCTCAAGATGACAAGTGTATCGCCATCCAGTATTTGGAAGCCATTCGGAATCTCAGAAATCGGGATTTCGTCCCTGTTCGTACGATTCATATCTCGTATGTACCGGATGAGGAGATTGGGGGTTCCGATGGAGCTGCGAAGTTCGTTCAGTCGAAGGAGTTCAAGGAGTTGAATGTGGGGTTTATGATGGATGAAGGACAAGCTTCACCGGGAGATGAGTTTAGGGTTTTTTACGCCGATAGATCGCCATGGTCGTTGATAATCAAGGCTAAAGGAAGCCCTGGCCATGGATCTAGAATGTACGATAACAGCGCAATGGAGAATTTGATGAAGAGCGTTGAGATTATGACTAGATTCAGAGAGAGCCAATTTGAAATAGTTAAAGCAGGTGAAGCTGCTAATTCAGAAGTCATCTCTGTGAATCCTGTTTTTGTCAATGCCGGAACTCCTTCCCCCACAGGATTTGTGATGAACATGCAACCGTCAGAGGCGGAGGCTGGGTTTGATCTCAGATTACCGCCGACCGCCGACCCAGATGCTATGAGAAGAAGAATAGCTGAGGAATGGGCTCCGGCACGGCGGAACATGACTTATCAGATACTTGAGAAAGGGCCCATAAGAGATTTCTTGGGAAGTCCATTAATGACGATGACAAATGCTTCAAATCCATGGTGGGCTGTTTTCAAGGCAGCCATTTCCGACGCTGGAGGGAAGCTTTCTAAGCCCGAAATCTTGGCTACAACTACGGATGCTCGATTCATGAGACAGATGAGGATTCCTGTTCTTGGTTTCTCTCCAATGATTAACACTCCAATCTTATTGCATGATCACAATGAGTTCTTGAGTGATTCTGTGTTCATAAGGGGAATCAAGGTTTATGAATCCTTAATCAGTGCTTTGAGTTCCTTCCAAGAGGATGTTTCTTCCCAATAATAAGCATTGCTCATCCTTCAGAATTCTCATGGGAATCAACACTGTAAAGTATCCATTCAGAAGGACAATCTCAAAGGCATAGCTTTATGCAAAAGTAAGCTTTCTTCCCAACCCGACCCAATTCACCGTTCGTGTCTATTTGGTCTTTAAACTTTAAATGGTTGTTTAGAATGCTTTTCATTTCATGGGTCTTCGGATACTTTCACGGAAAAAGAGTCGTATAAATGATAAATCTCAATGATATATGAAACTTTGTAGCATATTTTCATTGAGTTTGAGTTTATATTGTTGAATAAGACTGTTATGGTTGAGGAACTTAAATACTCTTCCACCCATCATTGTTATGGAGAATAAAACCTTGAAGTTTCTTTTGATGGAGTTTGTTAAATAGCTTTGTGGTTAAGGAACAACTCATTCATTACAAGAGTTGTTCAAAGTTACCTTTAAAATTGTATTGATAAACCAAACAAAATGAAAGGAGGCC

Coding sequence (CDS)

ATGTCGAACTTCCATCTTCACTATCGTCTCTTTTCAGTCACTCTTTATCTTCTTCTTCTTCTACTCTCATTTCCTCTCTCTTTCAGCCATTCACAGTACGTTGATGATGAACCCGATCAAGATTCTCCGGTCTCTCGCTTTCAACGCTACCTCAGAATCAAAACCGCTCATCCAGATCCCGATTACGCCTCAGCTGTCGCCTTCCTCAGATCTCAAGCACAAGAAATCGGTCTCCATACGCAGATACTCGAGTTCGTTACAGGTAAGCCTCTTCTTCTTGTGACCTGGTATGGATCAGACCCTTCTTTGCCTTCGATTTTACTCAACTCTCATATGGACTCTGTCCCCGCCGAGCCTTCTAAGTGGGTTCATCCTCCATTCTCCGCTGTCCGAACCTCTGATGGTAAAATCTTCGCCCGTGGTTCTCAAGATGACAAGTGTATCGCCATCCAGTATTTGGAAGCCATTCGGAATCTCAGAAATCGGGATTTCGTCCCTGTTCGTACGATTCATATCTCGTATGTACCGGATGAGGAGATTGGGGGTTCCGATGGAGCTGCGAAGTTCGTTCAGTCGAAGGAGTTCAAGGAGTTGAATGTGGGGTTTATGATGGATGAAGGACAAGCTTCACCGGGAGATGAGTTTAGGGTTTTTTACGCCGATAGATCGCCATGGTCGTTGATAATCAAGGCTAAAGGAAGCCCTGGCCATGGATCTAGAATGTACGATAACAGCGCAATGGAGAATTTGATGAAGAGCGTTGAGATTATGACTAGATTCAGAGAGAGCCAATTTGAAATAGTTAAAGCAGGTGAAGCTGCTAATTCAGAAGTCATCTCTGTGAATCCTGTTTTTGTCAATGCCGGAACTCCTTCCCCCACAGGATTTGTGATGAACATGCAACCGTCAGAGGCGGAGGCTGGGTTTGATCTCAGATTACCGCCGACCGCCGACCCAGATGCTATGAGAAGAAGAATAGCTGAGGAATGGGCTCCGGCACGGCGGAACATGACTTATCAGATACTTGAGAAAGGGCCCATAAGAGATTTCTTGGGAAGTCCATTAATGACGATGACAAATGCTTCAAATCCATGGTGGGCTGTTTTCAAGGCAGCCATTTCCGACGCTGGAGGGAAGCTTTCTAAGCCCGAAATCTTGGCTACAACTACGGATGCTCGATTCATGAGACAGATGAGGATTCCTGTTCTTGGTTTCTCTCCAATGATTAACACTCCAATCTTATTGCATGATCACAATGAGTTCTTGAGTGATTCTGTGTTCATAAGGGGAATCAAGGTTTATGAATCCTTAATCAGTGCTTTGAGTTCCTTCCAAGAGGATGTTTCTTCCCAATAA

Protein sequence

MSNFHLHYRLFSVTLYLLLLLLSFPLSFSHSQYVDDEPDQDSPVSRFQRYLRIKTAHPDPDYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVTWYGSDPSLPSILLNSHMDSVPAEPSKWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTIHISYVPDEEIGGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGSPGHGSRMYDNSAMENLMKSVEIMTRFRESQFEIVKAGEAANSEVISVNPVFVNAGTPSPTGFVMNMQPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPARRNMTYQILEKGPIRDFLGSPLMTMTNASNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNEFLSDSVFIRGIKVYESLISALSSFQEDVSSQ*

Homology

[Close]

BLAST of CSPI01G01600 vs. ExPASy Swiss-Prot
Match: Q99JW2 (Aminoacylase-1 OS=Mus musculus OX=10090 GN=Acy1 PE=1 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 9.3e-91
Identity = 174/409 (42.54%), Postives = 247/409 (60.39%), Query Frame = 0


Query: 37  EPDQDSP-VSRFQRYLRIKTAHPDPDYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVT 96
           +P+ + P V+ F++YLRI T  P+PDY  A+ FL  +A+++GL  Q +E V G  + ++T
Sbjct: 5   DPESEHPSVTLFRQYLRICTVQPNPDYGGAITFLEERARQLGLSCQKIEVVPGFVITVLT 64

Query: 97  WYGSDPSLPSILLNSHMDSVPAEPSKWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEA 156
           W G++PSLPSILLNSH D VP     W H PF A + S+G I+ARGSQD K ++IQYLEA
Sbjct: 65  WPGTNPSLPSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGSQDMKSVSIQYLEA 124

Query: 157 IRNLRNRDFVPVRTIHISYVPDEEIGGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEF 216
           +R L++      RTIH+++VPDEE+GG  G   FV+  EF+ L  GF +DEG A+P D F
Sbjct: 125 VRRLKSEGHRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDAF 184

Query: 217 RVFYADRSPWSLIIKAKGSPGHGSRMYDNSAMENLMKSVEIMTRFRESQFEIVKAG-EAA 276
            VFY++RSPW + + + G PGH SR  +++A E L K +  +  FRE + + ++A     
Sbjct: 185 TVFYSERSPWWVRVTSTGKPGHASRFIEDTAAEKLHKVISSILAFREKERQRLQANPHLK 244

Query: 277 NSEVISVNPVFVNAGTPSPTGFVMNMQPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPAR 336
              V SVN       T    G   N+ P+   A FD R+ P  D  A  +++      A 
Sbjct: 245 EGAVTSVNL------TKLEGGVAYNVVPATMSASFDFRVAPDVDMKAFEKQLQRWCQEAG 304

Query: 337 RNMTYQILEKGPIRDFLGSPLMTMTNASNPWWAVFKAAISDAGGKLSKPEILATTTDARF 396
             +T++  +K         P MT T+ S+PWWA F  A   A     +PEI    TD+R+
Sbjct: 305 EGVTFEFAQK------FTEPRMTPTDDSDPWWAAFSGACK-AMNLTLEPEIFPAATDSRY 364

Query: 397 MRQMRIPVLGFSPMINTPILLHDHNEFLSDSVFIRGIKVYESLISALSS 444
           +R + IP LGFSPM  TP+LLHDHNE L + +F+RG+ +Y  L+SAL+S
Sbjct: 365 IRAVGIPALGFSPMNRTPVLLHDHNERLHEDIFLRGVDIYTGLLSALAS 400

[Close]

BLAST of CSPI01G01600 vs. ExPASy Swiss-Prot
Match: P37111 (Aminoacylase-1 OS=Sus scrofa OX=9823 GN=ACY1 PE=1 SV=2)

HSP 1 Score: 331.6 bits (849), Expect = 1.3e-89
Identity = 168/400 (42.00%), Postives = 244/400 (61.00%), Query Frame = 0


Query: 44  VSRFQRYLRIKTAHPDPDYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVTWYGSDPSL 103
           V+ F++YLRI+T  P+PDY +AVAFL  +A+++GL  Q +E V G  + ++TW G++P+L
Sbjct: 13  VTLFRQYLRIRTVQPEPDYGAAVAFLEERARQLGLGCQKVEVVPGHVVTVLTWPGTNPTL 72

Query: 104 PSILLNSHMDSVPAEPSKWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRD 163
            SILLNSH D VP     W H PF   + +DG I+ RG+QD KC++IQYLEA+R L+   
Sbjct: 73  SSILLNSHTDVVPVFKEHWSHDPFEGFKDADGYIYGRGAQDMKCVSIQYLEAVRRLKVEG 132

Query: 164 FVPVRTIHISYVPDEEIGGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRS 223
               RTIH+++VPDEE+GG  G   FV+  EF+ L  GF +DEG ASP D F VFY++RS
Sbjct: 133 HHFPRTIHMTFVPDEEVGGHQGMELFVKRPEFQALRAGFALDEGLASPTDAFTVFYSERS 192

Query: 224 PWSLIIKAKGSPGHGSRMYDNSAMENLMKSVEIMTRFRESQFEIVKAGEAANSEVISVNP 283
           PW L + + G PGHGSR  +++A E L K +  +  FRE + + +++ +     V SVN 
Sbjct: 193 PWWLRVTSTGKPGHGSRFIEDTAAEKLHKVINSILAFREKEKQRLQSNQLKPGAVTSVNL 252

Query: 284 VFVNAGTPSPTGFVMNMQPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPARRNMTYQILE 343
             +  G         N+ P+   A FD R+ P  D  A   ++      A   +T++ ++
Sbjct: 253 TMLEGGV------AYNVVPATMSACFDFRVAPDVDLKAFEEQLQSWCQAAGEGVTFEFVQ 312

Query: 344 KGPIRDFLGSPLMTMTNASNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVL 403
           K           +T T+ S+PWWA F     D    L + EI   +TDAR++R   +P L
Sbjct: 313 KWM------ETQVTSTDDSDPWWAAFSGVFKDMKLAL-ELEICPASTDARYIRAAGVPAL 372

Query: 404 GFSPMINTPILLHDHNEFLSDSVFIRGIKVYESLISALSS 444
           GFSPM +TP+LLHDH+E L ++VF+RG+ +Y  L+SAL+S
Sbjct: 373 GFSPMNHTPVLLHDHDERLHEAVFLRGVDIYTQLLSALAS 399

[Close]

BLAST of CSPI01G01600 vs. ExPASy Swiss-Prot
Match: Q6AYS7 (Aminoacylase-1A OS=Rattus norvegicus OX=10116 GN=Acy1a PE=1 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 1.5e-88
Identity = 172/408 (42.16%), Postives = 246/408 (60.29%), Query Frame = 0


Query: 38  PDQDSP-VSRFQRYLRIKTAHPDPDYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVTW 97
           P+ + P V+ F++YLRI T  P+PDY SAV FL  +A+++GL  Q +E   G  + ++TW
Sbjct: 6   PESEHPSVTLFRQYLRICTVQPNPDYGSAVTFLEERARQLGLSCQKIEVAPGYVITVLTW 65

Query: 98  YGSDPSLPSILLNSHMDSVPAEPSKWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAI 157
            G++P L SILLNSH D VP     W H PF A + S+G I+ARG+QD K ++IQYLEA+
Sbjct: 66  PGTNPLLHSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGAQDMKSVSIQYLEAV 125

Query: 158 RNLRNRDFVPVRTIHISYVPDEEIGGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEFR 217
           R L++      RTIH+++VPDEE+GG  G   FV+  EF+ L  GF +DEG A+P D F 
Sbjct: 126 RRLKSEGHRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDAFT 185

Query: 218 VFYADRSPWSLIIKAKGSPGHGSRMYDNSAMENLMKSVEIMTRFRESQFEIVKAG-EAAN 277
           VFY++RSPW + + + G PGH SR  +++A E L K V  +  FRE + + ++A      
Sbjct: 186 VFYSERSPWWIRVTSTGKPGHASRFIEDTAAEKLHKVVNSILAFREKERQRLQANPHLKE 245

Query: 278 SEVISVNPVFVNAGTPSPTGFVMNMQPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPARR 337
             V SVN       T    G   N+ P+   A FD R+ P  D  A  +++      A  
Sbjct: 246 GAVTSVNL------TKLEGGVAYNVVPATMSACFDFRVAPDVDMKAFEKQLQSWCQEAGE 305

Query: 338 NMTYQILEKGPIRDFLGSPLMTMTNASNPWWAVFKAAISDAGGKLSKPEILATTTDARFM 397
            +T++  +K         P MT T+ ++PWWA F  A  +    L +PEI    TD+R++
Sbjct: 306 GVTFEFAQK------FTEPRMTPTDDTDPWWAAFSGACKEMNLTL-EPEIFPAATDSRYI 365

Query: 398 RQMRIPVLGFSPMINTPILLHDHNEFLSDSVFIRGIKVYESLISALSS 444
           R + IP LGFSPM  TP+LLHDHNE L ++VF+RG+ +Y  L++AL+S
Sbjct: 366 RAVGIPALGFSPMNRTPVLLHDHNERLHEAVFLRGVDIYTRLVAALAS 400

[Close]

BLAST of CSPI01G01600 vs. ExPASy Swiss-Prot
Match: Q6PTT0 (Aminoacylase-1B OS=Rattus norvegicus OX=10116 GN=Acy1b PE=1 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 1.5e-88
Identity = 170/408 (41.67%), Postives = 245/408 (60.05%), Query Frame = 0


Query: 38  PDQDSP-VSRFQRYLRIKTAHPDPDYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVTW 97
           P+++ P V+ F++YLRI+T  P PDY +AVAF    A+++GL  Q +E   G  + ++TW
Sbjct: 6   PEEEHPSVTLFRQYLRIRTVQPKPDYGAAVAFFEETARQLGLGCQKVEVAPGYVVTVLTW 65

Query: 98  YGSDPSLPSILLNSHMDSVPAEPSKWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAI 157
            G++P+L SILLNSH D VP     W H PF A + S+G I+ RG+QD KC++IQYLEA+
Sbjct: 66  PGTNPTLSSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYTRGAQDMKCVSIQYLEAV 125

Query: 158 RNLRNRDFVPVRTIHISYVPDEEIGGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEFR 217
           + L+       RTIH+++VPDEE+GG  G   FVQ  EF  L  GF +DEG A+P D F 
Sbjct: 126 KRLKVEGHRFPRTIHMTFVPDEEVGGHQGMELFVQRHEFHALRAGFALDEGLANPTDAFT 185

Query: 218 VFYADRSPWSLIIKAKGSPGHGSRMYDNSAMENLMKSVEIMTRFRESQFEIVKAG-EAAN 277
           VFY++RSPW + + + G PGH SR  +++A E L K V  +  FRE +++ +++      
Sbjct: 186 VFYSERSPWWVRVTSTGRPGHASRFMEDTAAEKLHKVVNSILAFREKEWQRLQSNPHLKE 245

Query: 278 SEVISVNPVFVNAGTPSPTGFVMNMQPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPARR 337
             V SVN       T    G   N+ P+   A FD R+ P  D  A   ++      A  
Sbjct: 246 GSVTSVNL------TKLEGGVAYNVVPATMSACFDFRVAPDVDMKAFEEQLQSWCQEAGE 305

Query: 338 NMTYQILEKGPIRDFLGSPLMTMTNASNPWWAVFKAAISDAGGKLSKPEILATTTDARFM 397
            +T++  +K         P MT T+ ++PWWA F  A  +    L +PEI    TD+R++
Sbjct: 306 GVTFEFAQK------FTEPRMTPTDDTDPWWAAFSGACKEMTLTL-EPEIFPAATDSRYI 365

Query: 398 RQMRIPVLGFSPMINTPILLHDHNEFLSDSVFIRGIKVYESLISALSS 444
           R + IP LGFSPM  TP+LLHDHNE L ++VF+RG+ +Y  L++AL+S
Sbjct: 366 RAVGIPALGFSPMNRTPVLLHDHNERLHEAVFLRGVDIYTRLVAALAS 400

[Close]

BLAST of CSPI01G01600 vs. ExPASy Swiss-Prot
Match: Q5RFB0 (Aminoacylase-1 OS=Pongo abelii OX=9601 GN=ACY1 PE=2 SV=2)

HSP 1 Score: 326.6 bits (836), Expect = 4.3e-88
Identity = 173/408 (42.40%), Postives = 244/408 (59.80%), Query Frame = 0


Query: 38  PDQDSP-VSRFQRYLRIKTAHPDPDYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVTW 97
           P+++ P V+ F++YLRI+T  P PDY +AVAF   +A+++GL  Q +E   G  + ++TW
Sbjct: 6   PEEEHPSVTLFRQYLRIRTVQPKPDYGAAVAFFEERARQLGLGCQKVEVAPGYVVTVLTW 65

Query: 98  YGSDPSLPSILLNSHMDSVPAEPSKWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAI 157
            G++P+L SILLNSH D VP     W H PF A + S+G I+ARG+QD KCI+IQYLEA+
Sbjct: 66  PGTNPTLSSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYARGAQDMKCISIQYLEAV 125

Query: 158 RNLRNRDFVPVRTIHISYVPDEEIGGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEFR 217
           R L+       RTIH+++VPDEE+GG  G   FVQ  EF  L  GF +DEG A+P D F 
Sbjct: 126 RRLKVEGHRFPRTIHMTFVPDEEVGGHQGMELFVQRPEFHALRAGFALDEGIANPTDAFT 185

Query: 218 VFYADRSPWSLIIKAKGSPGHGSRMYDNSAMENLMKSVEIMTRFRESQFEIVKAG-EAAN 277
           VFY++RSPW + + + G PGH SR  +++A E L K V  +  FRE +++ +++      
Sbjct: 186 VFYSERSPWWVRVTSTGRPGHASRFMEDTAAEKLHKVVSSILAFREKEWQRLQSNPHLKE 245

Query: 278 SEVISVNPVFVNAGTPSPTGFVMNMQPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPARR 337
             V SVN       T    G   N+ P+   A FD R+ P  D  A   ++      A  
Sbjct: 246 GSVTSVNL------TKLEGGVAYNVIPATMSASFDFRVAPDVDFKAFEEQLQSWCQAAGE 305

Query: 338 NMTYQILEKGPIRDFLGSPLMTMTNASNPWWAVFKAAISDAGGKLSKPEILATTTDARFM 397
            +T +  +K         P +T T+ SNPWWA F     D    L +PEI+   TD R++
Sbjct: 306 GVTLEFAQKWM------HPQVTPTDDSNPWWAAFSRVCKDMKLTL-EPEIMPAATDNRYI 365

Query: 398 RQMRIPVLGFSPMINTPILLHDHNEFLSDSVFIRGIKVYESLISALSS 444
           R + IP LGFSPM  TP+LLHDH+E L ++VF+RG+ +Y  L+ AL+S
Sbjct: 366 RAVGIPALGFSPMNRTPVLLHDHDERLHEAVFLRGVDIYTRLLPALAS 400

[Close]

BLAST of CSPI01G01600 vs. ExPASy TrEMBL
Match: A0A0A0LRI1 (M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G005600 PE=4 SV=1)

HSP 1 Score: 897.9 bits (2319), Expect = 1.7e-257
Identity = 448/451 (99.33%), Postives = 449/451 (99.56%), Query Frame = 0


Query: 1   MSNFHLHYRLFSVTLYLLLLLLSFPLSFSHSQYVDDEPDQDSPVSRFQRYLRIKTAHPDP 60
           MSNFHLHYRLFSVTLYLLLLLLSFPLSFS SQYVDDEPDQDSPVSRFQRYLRIKTAHPDP
Sbjct: 1   MSNFHLHYRLFSVTLYLLLLLLSFPLSFSQSQYVDDEPDQDSPVSRFQRYLRIKTAHPDP 60

Query: 61  DYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVTWYGSDPSLPSILLNSHMDSVPAEPS 120
           DYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVTWYG DPSLPSILLNSHMDSVPAEPS
Sbjct: 61  DYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVTWYGLDPSLPSILLNSHMDSVPAEPS 120

Query: 121 KWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTIHISYVPDEEI 180
           KWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTIHISYVPDEEI
Sbjct: 121 KWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTIHISYVPDEEI 180

Query: 181 GGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGSPGHGSR 240
           GGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGSPGHGSR
Sbjct: 181 GGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGSPGHGSR 240

Query: 241 MYDNSAMENLMKSVEIMTRFRESQFEIVKAGEAANSEVISVNPVFVNAGTPSPTGFVMNM 300
           MYDNSAMENLMKSVEIMTRFRESQFEIVKAGEAANSEVISVNPVFVNAGTPSPTGFVMNM
Sbjct: 241 MYDNSAMENLMKSVEIMTRFRESQFEIVKAGEAANSEVISVNPVFVNAGTPSPTGFVMNM 300

Query: 301 QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPARRNMTYQILEKGPIRDFLGSPLMTMTN 360
           QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPARRNMTYQILEKGPIRD+LGSPLMTMTN
Sbjct: 301 QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPARRNMTYQILEKGPIRDYLGSPLMTMTN 360

Query: 361 ASNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE 420
           ASNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE
Sbjct: 361 ASNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE 420

Query: 421 FLSDSVFIRGIKVYESLISALSSFQEDVSSQ 452
           FLSDSVFIRGIKVYESLISALSSFQEDVSSQ
Sbjct: 421 FLSDSVFIRGIKVYESLISALSSFQEDVSSQ 451

[Close]

BLAST of CSPI01G01600 vs. ExPASy TrEMBL
Match: A0A5D3BIQ8 (Aminoacylase-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001910 PE=4 SV=1)

HSP 1 Score: 859.4 bits (2219), Expect = 6.9e-246
Identity = 426/451 (94.46%), Postives = 439/451 (97.34%), Query Frame = 0


Query: 1   MSNFHLHYRLFSVTLYLLLLLLSFPLSFSHSQYVDDEPDQDSPVSRFQRYLRIKTAHPDP 60
           MSNF LH+R FSVTLY  LLLLSFPLSFSHSQYVDDE DQDSPVSRFQRYLRIKTAHPDP
Sbjct: 1   MSNFRLHHRFFSVTLY--LLLLSFPLSFSHSQYVDDESDQDSPVSRFQRYLRIKTAHPDP 60

Query: 61  DYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVTWYGSDPSLPSILLNSHMDSVPAEPS 120
           DYASAVAFLRSQAQEIGLHT++LEF  GKPLLLVTWYGS+PSLPS+LLNSHMDSVPAEPS
Sbjct: 61  DYASAVAFLRSQAQEIGLHTEVLEFGPGKPLLLVTWYGSNPSLPSVLLNSHMDSVPAEPS 120

Query: 121 KWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTIHISYVPDEEI 180
           KW+HPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRT+HISYVPDEEI
Sbjct: 121 KWLHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTVHISYVPDEEI 180

Query: 181 GGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGSPGHGSR 240
           GGSDG AKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKG+PGHGSR
Sbjct: 181 GGSDGVAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGTPGHGSR 240

Query: 241 MYDNSAMENLMKSVEIMTRFRESQFEIVKAGEAANSEVISVNPVFVNAGTPSPTGFVMNM 300
           MYDNSAMENLMKSVEI++RFRESQFEIVKAGEAANSEVISVNPVFVNAG PSPTGFVMNM
Sbjct: 241 MYDNSAMENLMKSVEIVSRFRESQFEIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNM 300

Query: 301 QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPARRNMTYQILEKGPIRDFLGSPLMTMTN 360
           QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPA RNMTYQI+EKGP+RD+LGSPLMTMTN
Sbjct: 301 QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPAWRNMTYQIIEKGPLRDYLGSPLMTMTN 360

Query: 361 ASNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE 420
            SNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE
Sbjct: 361 DSNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE 420

Query: 421 FLSDSVFIRGIKVYESLISALSSFQEDVSSQ 452
           FLSDSVFIRGIKVYESLIS LSSFQEDVSSQ
Sbjct: 421 FLSDSVFIRGIKVYESLISTLSSFQEDVSSQ 449

[Close]

BLAST of CSPI01G01600 vs. ExPASy TrEMBL
Match: A0A5A7URV3 (Aminoacylase-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G001820 PE=4 SV=1)

HSP 1 Score: 859.0 bits (2218), Expect = 9.0e-246
Identity = 425/451 (94.24%), Postives = 440/451 (97.56%), Query Frame = 0


Query: 1   MSNFHLHYRLFSVTLYLLLLLLSFPLSFSHSQYVDDEPDQDSPVSRFQRYLRIKTAHPDP 60
           MSNF LH+R FSVTLY  LLLLSFPLSFSHSQYVDDE DQDSPVSRFQRYLRIKTAHPDP
Sbjct: 85  MSNFRLHHRFFSVTLY--LLLLSFPLSFSHSQYVDDESDQDSPVSRFQRYLRIKTAHPDP 144

Query: 61  DYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVTWYGSDPSLPSILLNSHMDSVPAEPS 120
           DYASAVAFLRSQAQEIGLHT++LEF  GKPLLLVTWYGS+PSLPS+LLNSHMDSVPAEPS
Sbjct: 145 DYASAVAFLRSQAQEIGLHTEVLEFGPGKPLLLVTWYGSNPSLPSVLLNSHMDSVPAEPS 204

Query: 121 KWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTIHISYVPDEEI 180
           KW+HPPFSAVRTSDGKIFARGSQDDKCIAIQY+EAIRNLRNRDFVPVRT+HISYVPDEEI
Sbjct: 205 KWLHPPFSAVRTSDGKIFARGSQDDKCIAIQYIEAIRNLRNRDFVPVRTVHISYVPDEEI 264

Query: 181 GGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGSPGHGSR 240
           GGSDG AKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKG+PGHGSR
Sbjct: 265 GGSDGVAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGTPGHGSR 324

Query: 241 MYDNSAMENLMKSVEIMTRFRESQFEIVKAGEAANSEVISVNPVFVNAGTPSPTGFVMNM 300
           MYDNSAMENLMKSVEI++RFRESQFEIVKAGEAANSEVISVNPVFVNAG PSPTGFVMNM
Sbjct: 325 MYDNSAMENLMKSVEIISRFRESQFEIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNM 384

Query: 301 QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPARRNMTYQILEKGPIRDFLGSPLMTMTN 360
           QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPA RNMTYQI+EKGP+RD+LGSPLMTMTN
Sbjct: 385 QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPAWRNMTYQIIEKGPLRDYLGSPLMTMTN 444

Query: 361 ASNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE 420
            SNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE
Sbjct: 445 DSNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE 504

Query: 421 FLSDSVFIRGIKVYESLISALSSFQEDVSSQ 452
           FLSDSVFIRGIKVYESLIS+LSSFQEDVSSQ
Sbjct: 505 FLSDSVFIRGIKVYESLISSLSSFQEDVSSQ 533

[Close]

BLAST of CSPI01G01600 vs. ExPASy TrEMBL
Match: A0A6J1JRT3 (aminoacylase-1 OS=Cucurbita maxima OX=3661 GN=LOC111487760 PE=4 SV=1)

HSP 1 Score: 776.9 bits (2005), Expect = 4.5e-221
Identity = 379/449 (84.41%), Postives = 417/449 (92.87%), Query Frame = 0


Query: 1   MSNFHLHYRLFSVTLYLLLLLLSFPLSFSHSQYVDDEPDQDSPVSRFQRYLRIKTAHPDP 60
           M+NF LH RLFS+ ++L L L  FPLSFSHSQY DDE DQ+SP+SRFQ YLRIKTAHPDP
Sbjct: 1   MANFRLHCRLFSLAVFLFLFL--FPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDP 60

Query: 61  DYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVTWYGSDPSLPSILLNSHMDSVPAEPS 120
           DYASAV+FL SQA EIGLHT+ILEFV GKPLLLVTW G +PSLPSILLNSHMDSVPAEPS
Sbjct: 61  DYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPS 120

Query: 121 KWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTIHISYVPDEEI 180
           KW++PPFSA+RTSDGKIFARGSQDDKCIAIQYLEAIRNLR+R+F+P RTIHISYVPDEEI
Sbjct: 121 KWLYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFIPDRTIHISYVPDEEI 180

Query: 181 GGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGSPGHGSR 240
           GG DGA+KFV SKEFKELNVGFMMDEGQASPGDEFRVFYADRSPW LII+A+G+PGHGSR
Sbjct: 181 GGFDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGAPGHGSR 240

Query: 241 MYDNSAMENLMKSVEIMTRFRESQFEIVKAGEAANSEVISVNPVFVNAGTPSPTGFVMNM 300
           MYDNSAMENLMKSVEI+++FRESQF++VKAGE ANSEVISVNPVFVNAG PSPTGFVMNM
Sbjct: 241 MYDNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVISVNPVFVNAGIPSPTGFVMNM 300

Query: 301 QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPARRNMTYQILEKGPIRDFLGSPLMTMTN 360
           QPSEAEAGFDLR+PPTADPDA+RRRI EEWAPARRN+T QI+EKGPIRD+LG PLMTMTN
Sbjct: 301 QPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTN 360

Query: 361 ASNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE 420
            SNPWW+VFK AISDAGGKLS+PEILA+TTDARFMRQ+ IPVLGFSPM NTPILLH+HNE
Sbjct: 361 DSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNE 420

Query: 421 FLSDSVFIRGIKVYESLISALSSFQEDVS 450
           FL D+VF+RGIK+YES+ISALSSF  D S
Sbjct: 421 FLKDTVFLRGIKIYESIISALSSFVADAS 447

[Close]

BLAST of CSPI01G01600 vs. ExPASy TrEMBL
Match: A0A6J1HLT6 (aminoacylase-1 OS=Cucurbita moschata OX=3662 GN=LOC111464784 PE=4 SV=1)

HSP 1 Score: 775.0 bits (2000), Expect = 1.7e-220
Identity = 379/449 (84.41%), Postives = 417/449 (92.87%), Query Frame = 0


Query: 1   MSNFHLHYRLFSVTLYLLLLLLSFPLSFSHSQYVDDEPDQDSPVSRFQRYLRIKTAHPDP 60
           M+NF LH RLFS+ ++  L L SFPLSFSHSQY DDE DQ+SP+SRFQ YLRIKTAHPDP
Sbjct: 1   MANFRLHCRLFSLAVF--LFLFSFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDP 60

Query: 61  DYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVTWYGSDPSLPSILLNSHMDSVPAEPS 120
           DYASAV+FL SQA EIGLHT+ILEFV GKPLLLVTW G +PSLPSILLNSHMDSVPAEPS
Sbjct: 61  DYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPS 120

Query: 121 KWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTIHISYVPDEEI 180
           KW++PPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIR+LR+R+F+P RTIHISYVPDEEI
Sbjct: 121 KWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSLRSRNFIPDRTIHISYVPDEEI 180

Query: 181 GGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGSPGHGSR 240
           GG DGAAKFV SKEFKELNVGFMMDEGQASPGDEFRVFYADRSPW L I+A+G+PGHGSR
Sbjct: 181 GGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLKIRAEGTPGHGSR 240

Query: 241 MYDNSAMENLMKSVEIMTRFRESQFEIVKAGEAANSEVISVNPVFVNAGTPSPTGFVMNM 300
           MYDNSAMENLMKSVEI+++FRESQF++VKAGEAANSEVISVNPVFVNAG PSPTGFVMNM
Sbjct: 241 MYDNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNM 300

Query: 301 QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPARRNMTYQILEKGPIRDFLGSPLMTMTN 360
           QPSEAEAGFDLR+PPTADPDA+RRRI EEWAPARRN+T QI+EKGPIRD+LG PL+TMTN
Sbjct: 301 QPSEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTN 360

Query: 361 ASNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE 420
            SNPWW+VFK AISDAGGKLS+PEILA+TTDARFMRQ+ IPVLGFSPM NTPILLH+HNE
Sbjct: 361 DSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNE 420

Query: 421 FLSDSVFIRGIKVYESLISALSSFQEDVS 450
           FL D+VF+RGIK+YES+ISALSSF  D S
Sbjct: 421 FLKDTVFLRGIKIYESIISALSSFVADAS 447

[Close]

BLAST of CSPI01G01600 vs. NCBI nr
Match: KGN63599.1 (hypothetical protein Csa_013948 [Cucumis sativus])

HSP 1 Score: 897.9 bits (2319), Expect = 3.6e-257
Identity = 448/451 (99.33%), Postives = 449/451 (99.56%), Query Frame = 0


Query: 1   MSNFHLHYRLFSVTLYLLLLLLSFPLSFSHSQYVDDEPDQDSPVSRFQRYLRIKTAHPDP 60
           MSNFHLHYRLFSVTLYLLLLLLSFPLSFS SQYVDDEPDQDSPVSRFQRYLRIKTAHPDP
Sbjct: 1   MSNFHLHYRLFSVTLYLLLLLLSFPLSFSQSQYVDDEPDQDSPVSRFQRYLRIKTAHPDP 60

Query: 61  DYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVTWYGSDPSLPSILLNSHMDSVPAEPS 120
           DYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVTWYG DPSLPSILLNSHMDSVPAEPS
Sbjct: 61  DYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVTWYGLDPSLPSILLNSHMDSVPAEPS 120

Query: 121 KWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTIHISYVPDEEI 180
           KWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTIHISYVPDEEI
Sbjct: 121 KWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTIHISYVPDEEI 180

Query: 181 GGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGSPGHGSR 240
           GGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGSPGHGSR
Sbjct: 181 GGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGSPGHGSR 240

Query: 241 MYDNSAMENLMKSVEIMTRFRESQFEIVKAGEAANSEVISVNPVFVNAGTPSPTGFVMNM 300
           MYDNSAMENLMKSVEIMTRFRESQFEIVKAGEAANSEVISVNPVFVNAGTPSPTGFVMNM
Sbjct: 241 MYDNSAMENLMKSVEIMTRFRESQFEIVKAGEAANSEVISVNPVFVNAGTPSPTGFVMNM 300

Query: 301 QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPARRNMTYQILEKGPIRDFLGSPLMTMTN 360
           QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPARRNMTYQILEKGPIRD+LGSPLMTMTN
Sbjct: 301 QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPARRNMTYQILEKGPIRDYLGSPLMTMTN 360

Query: 361 ASNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE 420
           ASNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE
Sbjct: 361 ASNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE 420

Query: 421 FLSDSVFIRGIKVYESLISALSSFQEDVSSQ 452
           FLSDSVFIRGIKVYESLISALSSFQEDVSSQ
Sbjct: 421 FLSDSVFIRGIKVYESLISALSSFQEDVSSQ 451

[Close]

BLAST of CSPI01G01600 vs. NCBI nr
Match: TYJ98571.1 (aminoacylase-1 [Cucumis melo var. makuwa])

HSP 1 Score: 859.4 bits (2219), Expect = 1.4e-245
Identity = 426/451 (94.46%), Postives = 439/451 (97.34%), Query Frame = 0


Query: 1   MSNFHLHYRLFSVTLYLLLLLLSFPLSFSHSQYVDDEPDQDSPVSRFQRYLRIKTAHPDP 60
           MSNF LH+R FSVTLY  LLLLSFPLSFSHSQYVDDE DQDSPVSRFQRYLRIKTAHPDP
Sbjct: 1   MSNFRLHHRFFSVTLY--LLLLSFPLSFSHSQYVDDESDQDSPVSRFQRYLRIKTAHPDP 60

Query: 61  DYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVTWYGSDPSLPSILLNSHMDSVPAEPS 120
           DYASAVAFLRSQAQEIGLHT++LEF  GKPLLLVTWYGS+PSLPS+LLNSHMDSVPAEPS
Sbjct: 61  DYASAVAFLRSQAQEIGLHTEVLEFGPGKPLLLVTWYGSNPSLPSVLLNSHMDSVPAEPS 120

Query: 121 KWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTIHISYVPDEEI 180
           KW+HPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRT+HISYVPDEEI
Sbjct: 121 KWLHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTVHISYVPDEEI 180

Query: 181 GGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGSPGHGSR 240
           GGSDG AKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKG+PGHGSR
Sbjct: 181 GGSDGVAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGTPGHGSR 240

Query: 241 MYDNSAMENLMKSVEIMTRFRESQFEIVKAGEAANSEVISVNPVFVNAGTPSPTGFVMNM 300
           MYDNSAMENLMKSVEI++RFRESQFEIVKAGEAANSEVISVNPVFVNAG PSPTGFVMNM
Sbjct: 241 MYDNSAMENLMKSVEIVSRFRESQFEIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNM 300

Query: 301 QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPARRNMTYQILEKGPIRDFLGSPLMTMTN 360
           QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPA RNMTYQI+EKGP+RD+LGSPLMTMTN
Sbjct: 301 QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPAWRNMTYQIIEKGPLRDYLGSPLMTMTN 360

Query: 361 ASNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE 420
            SNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE
Sbjct: 361 DSNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE 420

Query: 421 FLSDSVFIRGIKVYESLISALSSFQEDVSSQ 452
           FLSDSVFIRGIKVYESLIS LSSFQEDVSSQ
Sbjct: 421 FLSDSVFIRGIKVYESLISTLSSFQEDVSSQ 449

[Close]

BLAST of CSPI01G01600 vs. NCBI nr
Match: KAA0057884.1 (aminoacylase-1 [Cucumis melo var. makuwa])

HSP 1 Score: 859.0 bits (2218), Expect = 1.9e-245
Identity = 425/451 (94.24%), Postives = 440/451 (97.56%), Query Frame = 0


Query: 1   MSNFHLHYRLFSVTLYLLLLLLSFPLSFSHSQYVDDEPDQDSPVSRFQRYLRIKTAHPDP 60
           MSNF LH+R FSVTLY  LLLLSFPLSFSHSQYVDDE DQDSPVSRFQRYLRIKTAHPDP
Sbjct: 85  MSNFRLHHRFFSVTLY--LLLLSFPLSFSHSQYVDDESDQDSPVSRFQRYLRIKTAHPDP 144

Query: 61  DYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVTWYGSDPSLPSILLNSHMDSVPAEPS 120
           DYASAVAFLRSQAQEIGLHT++LEF  GKPLLLVTWYGS+PSLPS+LLNSHMDSVPAEPS
Sbjct: 145 DYASAVAFLRSQAQEIGLHTEVLEFGPGKPLLLVTWYGSNPSLPSVLLNSHMDSVPAEPS 204

Query: 121 KWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTIHISYVPDEEI 180
           KW+HPPFSAVRTSDGKIFARGSQDDKCIAIQY+EAIRNLRNRDFVPVRT+HISYVPDEEI
Sbjct: 205 KWLHPPFSAVRTSDGKIFARGSQDDKCIAIQYIEAIRNLRNRDFVPVRTVHISYVPDEEI 264

Query: 181 GGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGSPGHGSR 240
           GGSDG AKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKG+PGHGSR
Sbjct: 265 GGSDGVAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGTPGHGSR 324

Query: 241 MYDNSAMENLMKSVEIMTRFRESQFEIVKAGEAANSEVISVNPVFVNAGTPSPTGFVMNM 300
           MYDNSAMENLMKSVEI++RFRESQFEIVKAGEAANSEVISVNPVFVNAG PSPTGFVMNM
Sbjct: 325 MYDNSAMENLMKSVEIISRFRESQFEIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNM 384

Query: 301 QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPARRNMTYQILEKGPIRDFLGSPLMTMTN 360
           QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPA RNMTYQI+EKGP+RD+LGSPLMTMTN
Sbjct: 385 QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPAWRNMTYQIIEKGPLRDYLGSPLMTMTN 444

Query: 361 ASNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE 420
            SNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE
Sbjct: 445 DSNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE 504

Query: 421 FLSDSVFIRGIKVYESLISALSSFQEDVSSQ 452
           FLSDSVFIRGIKVYESLIS+LSSFQEDVSSQ
Sbjct: 505 FLSDSVFIRGIKVYESLISSLSSFQEDVSSQ 533

[Close]

BLAST of CSPI01G01600 vs. NCBI nr
Match: XP_038879728.1 (aminoacylase-1-like [Benincasa hispida])

HSP 1 Score: 822.0 bits (2122), Expect = 2.5e-234
Identity = 404/451 (89.58%), Postives = 431/451 (95.57%), Query Frame = 0


Query: 1   MSNFHLHYRLFSVTLYLLLLLLSFPLSFSHSQYVDDEPDQDSPVSRFQRYLRIKTAHPDP 60
           M+NFHLHYRLF V  +   LLLSFPLSFSHSQY D E DQDSP+SRFQRYLRIKTAHPDP
Sbjct: 1   MANFHLHYRLFPVAAF--FLLLSFPLSFSHSQYGDGETDQDSPISRFQRYLRIKTAHPDP 60

Query: 61  DYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVTWYGSDPSLPSILLNSHMDSVPAEPS 120
           DYASAVAFL SQA EIGL+T+ILEFV GKPLLLVTWYGS+PSLPSILLNSHMDSVPAEPS
Sbjct: 61  DYASAVAFLSSQALEIGLNTRILEFVPGKPLLLVTWYGSNPSLPSILLNSHMDSVPAEPS 120

Query: 121 KWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTIHISYVPDEEI 180
           KW+HPPFSAVRTSDG+IFARGSQDDKCIAIQYLEAIRNL++R+F+PVRTIHISYVPDEEI
Sbjct: 121 KWLHPPFSAVRTSDGRIFARGSQDDKCIAIQYLEAIRNLKSRNFIPVRTIHISYVPDEEI 180

Query: 181 GGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGSPGHGSR 240
           GG DGAAKFV S+EFKELNVGFMMDEGQASPGDEFRVFYADRSPW LIIKAKG+PGHGSR
Sbjct: 181 GGFDGAAKFVLSQEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIKAKGTPGHGSR 240

Query: 241 MYDNSAMENLMKSVEIMTRFRESQFEIVKAGEAANSEVISVNPVFVNAGTPSPTGFVMNM 300
           MYDNSA+ENLMKSVEI++RFRESQFEIVKAGEAANSEVISVNPVFVNAG PSPTGFVMNM
Sbjct: 241 MYDNSAIENLMKSVEIVSRFRESQFEIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNM 300

Query: 301 QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPARRNMTYQILEKGPIRDFLGSPLMTMTN 360
           QPSEAEAGFDLRLPPTADPDA+RRRIAEEWAPARRNMT+QI+EKGPIRD+LG PLMTMTN
Sbjct: 301 QPSEAEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQIIEKGPIRDYLGGPLMTMTN 360

Query: 361 ASNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE 420
            SNPWW+VF+ AIS+AGGKLSKPEILATTTDARFMRQ+RIPVLGFSPMINTPILLHDHNE
Sbjct: 361 GSNPWWSVFRTAISEAGGKLSKPEILATTTDARFMRQLRIPVLGFSPMINTPILLHDHNE 420

Query: 421 FLSDSVFIRGIKVYESLISALSSFQEDVSSQ 452
           FL DSVF+RGIKVYESLISALSSFQ+DVSSQ
Sbjct: 421 FLRDSVFLRGIKVYESLISALSSFQKDVSSQ 449

[Close]

BLAST of CSPI01G01600 vs. NCBI nr
Match: KAG6602086.1 (Aminoacylase-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 777.7 bits (2007), Expect = 5.4e-221
Identity = 381/449 (84.86%), Postives = 417/449 (92.87%), Query Frame = 0


Query: 1   MSNFHLHYRLFSVTLYLLLLLLSFPLSFSHSQYVDDEPDQDSPVSRFQRYLRIKTAHPDP 60
           M+NF LH RLFS+ ++  L L SF LSFSHSQY DDE DQ+SP+SRFQ YLRIKTAHPDP
Sbjct: 1   MANFRLHCRLFSLAVF--LFLFSFSLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDP 60

Query: 61  DYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVTWYGSDPSLPSILLNSHMDSVPAEPS 120
           DYASAV+FL SQA EIGLHT+ILEFV GKPLLLVTW G +PSLPSILLNSHMDSVPAEPS
Sbjct: 61  DYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPS 120

Query: 121 KWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTIHISYVPDEEI 180
           KW++PPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLR+R+FVP RTIHISYVPDEEI
Sbjct: 121 KWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPDRTIHISYVPDEEI 180

Query: 181 GGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGSPGHGSR 240
           GG DGAAKFV SKEFKELNVGFMMDEGQASPGDEFRVFYADRSPW LII+A+G+PGHGSR
Sbjct: 181 GGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGTPGHGSR 240

Query: 241 MYDNSAMENLMKSVEIMTRFRESQFEIVKAGEAANSEVISVNPVFVNAGTPSPTGFVMNM 300
           MYDNSAMENLMKSVEI+++FRESQF++VKAGEAANSEVISVNPVFVNAG PSPTGFVMNM
Sbjct: 241 MYDNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNM 300

Query: 301 QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPARRNMTYQILEKGPIRDFLGSPLMTMTN 360
           QPSEAEAGFDLR+PPTADPDA+RRRI EEWAPARRN+T QI+EKGPIRD+LG PL+TMTN
Sbjct: 301 QPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTN 360

Query: 361 ASNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE 420
            SNPWW+VFK AISDAGGKLS+PEILA+TTDARFMRQ+ IPVLGFSPM NTPILLH+HNE
Sbjct: 361 DSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAIPVLGFSPMSNTPILLHEHNE 420

Query: 421 FLSDSVFIRGIKVYESLISALSSFQEDVS 450
           FL D+VF+RGIK+YES+ISALSSF  D S
Sbjct: 421 FLKDTVFLRGIKIYESIISALSSFVADAS 447

[Close]

BLAST of CSPI01G01600 vs. TAIR 10
Match: AT1G44820.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 623.6 bits (1607), Expect = 1.2e-178
Identity = 291/429 (67.83%), Postives = 360/429 (83.92%), Query Frame = 0


Query: 17  LLLLLLSFPLSFSHSQYVDDEPDQDSPVSRFQRYLRIKTAHPDPDYASAVAFLRSQAQEI 76
           LL  L+ F + FS         ++D+P++RFQ+YLR  TAHP+P+Y + ++FL +QAQ I
Sbjct: 6   LLWTLIIFSIIFS---LQSSSSEEDTPITRFQQYLRFNTAHPNPNYTAPISFLINQAQSI 65

Query: 77  GLHTQILEFVTGKPLLLVTWYGSDPSLPSILLNSHMDSVPAEPSKWVHPPFSAVRTSDGK 136
           GL T+ +EF++GKP+LL+TW GS+P+LPSIL NSH+DSVPAE  KW +PPFSA +T DG 
Sbjct: 66  GLTTKTIEFISGKPILLITWLGSNPNLPSILFNSHLDSVPAESEKWTYPPFSAHKTIDGH 125

Query: 137 IFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTIHISYVPDEEIGGSDGAAKFVQSKEFK 196
           I+ARG+QDDKCI +QYLE+IRNL++R F P+RTIHISYVP+EEIGG DG  KF  S EFK
Sbjct: 126 IYARGAQDDKCIGVQYLESIRNLKSRGFSPLRTIHISYVPEEEIGGFDGMMKFAASSEFK 185

Query: 197 ELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGSPGHGSRMYDNSAMENLMKSVEI 256
           +LN+GF MDEGQA+PGDEFRVFYADR PW  +IKA+G PGHG+++YDNSAMENLMKSVE+
Sbjct: 186 DLNLGFAMDEGQANPGDEFRVFYADRVPWHFVIKAEGIPGHGAKLYDNSAMENLMKSVEL 245

Query: 257 MTRFRESQFEIVKAGEAANSEVISVNPVFVNAGTPSPTGFVMNMQPSEAEAGFDLRLPPT 316
           ++RFRESQF+ VKAG+AA SEVISVNPV++ AGTP+ TGFVMNMQPSEAEAG+DLRLPP 
Sbjct: 246 ISRFRESQFDFVKAGKAAYSEVISVNPVYLKAGTPTTTGFVMNMQPSEAEAGYDLRLPPM 305

Query: 317 ADPDAMRRRIAEEWAPARRNMTYQILEKGPIRDFLGSPLMTMTNASNPWWAVFKAAISDA 376
           ADPD M++RIAEEWAP+ RNMTY I EKG +RD LG P+MT  N SNPWW++FK A+   
Sbjct: 306 ADPDVMKKRIAEEWAPSIRNMTYSIQEKGKLRDHLGRPIMTPVNDSNPWWSIFKQAVEAM 365

Query: 377 GGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNEFLSDSVFIRGIKVYES 436
           GGKL+KPEILA+TTD+RF+R + IP  GFSPM NTPILLHDHNEFL D+VF++GI+VYES
Sbjct: 366 GGKLAKPEILASTTDSRFIRTLGIPTFGFSPMTNTPILLHDHNEFLKDTVFMKGIEVYES 425

Query: 437 LISALSSFQ 446
           +ISALSSF+
Sbjct: 426 VISALSSFE 431

[Close]

BLAST of CSPI01G01600 vs. TAIR 10
Match: AT1G44180.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 615.1 bits (1585), Expect = 4.3e-176
Identity = 290/428 (67.76%), Postives = 360/428 (84.11%), Query Frame = 0


Query: 19  LLLLSFPLSF-SHSQYVDDEPDQDSPVSRFQRYLRIKTAHPDPDYASAVAFLRSQAQEIG 78
           L L SF  S  SHS+    E ++D+P++RFQ+YLR  TAHP+P+Y + ++FL  QA+ IG
Sbjct: 10  LFLFSFIFSLQSHSK----EEEEDTPITRFQQYLRFNTAHPNPNYTAPISFLLDQARSIG 69

Query: 79  LHTQILEFVTGKPLLLVTWYGSDPSLPSILLNSHMDSVPAEPSKWVHPPFSAVRTSDGKI 138
           L ++ +EFV GKP+LL+TW GS+ +L SIL NSH+DSVPAE  KW+HPPFSA RT DG I
Sbjct: 70  LTSRTIEFVPGKPVLLLTWLGSNLNLSSILFNSHLDSVPAESEKWIHPPFSAHRTIDGHI 129

Query: 139 FARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTIHISYVPDEEIGGSDGAAKFVQSKEFKE 198
           +ARG+QDDKCI +QYLEAIRNL++R F P+RTIHISYVP+EEIGG  G  KF  S EFK+
Sbjct: 130 YARGAQDDKCIGVQYLEAIRNLKSRSFSPLRTIHISYVPEEEIGGFGGMMKFAASSEFKD 189

Query: 199 LNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGSPGHGSRMYDNSAMENLMKSVEIM 258
           LN+GF+MDEGQASPGDEFRVFYA+R+PW L I+A G PGHG+++YDNSAMENLMKSVE++
Sbjct: 190 LNLGFVMDEGQASPGDEFRVFYAERTPWHLAIRANGMPGHGAKLYDNSAMENLMKSVELI 249

Query: 259 TRFRESQFEIVKAGEAANSEVISVNPVFVNAGTPSPTGFVMNMQPSEAEAGFDLRLPPTA 318
           +RFRESQF+ VKAG+AANSEVISVNPV++ AGTPS TGFVMNMQPSEAE G+DLRLPP A
Sbjct: 250 SRFRESQFDFVKAGKAANSEVISVNPVYLKAGTPSTTGFVMNMQPSEAEVGYDLRLPPMA 309

Query: 319 DPDAMRRRIAEEWAPARRNMTYQILEKGPIRDFLGSPLMTMTNASNPWWAVFKAAISDAG 378
           DP A+++RIAEEWAP+ RNMTY + ++G + D LG P+MT TN +NPWW++FK A+   G
Sbjct: 310 DPVALKKRIAEEWAPSIRNMTYTLKQQGKLTDHLGRPIMTTTNDTNPWWSIFKQAVEATG 369

Query: 379 GKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNEFLSDSVFIRGIKVYESL 438
           GKL+KPEIL +TTD+RF+R + IPVLGFSPMINTPIL+HDHNEFL D+VF++GI VYES+
Sbjct: 370 GKLAKPEILISTTDSRFIRTLGIPVLGFSPMINTPILVHDHNEFLKDTVFMKGIGVYESV 429

Query: 439 ISALSSFQ 446
           ISALSSF+
Sbjct: 430 ISALSSFE 433

[Close]

BLAST of CSPI01G01600 vs. TAIR 10
Match: AT4G38220.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 495.0 bits (1273), Expect = 6.5e-140
Identity = 228/411 (55.47%), Postives = 304/411 (73.97%), Query Frame = 0


Query: 41  DSPVSRFQRYLRIKTAHPDPDYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVTWYGSD 100
           D+ VSRFQ YLRI T  P+P+Y  AV F+ SQA+ + L +Q +EFV GKPLLL+ W GSD
Sbjct: 22  DAIVSRFQEYLRINTVQPNPEYYKAVDFIISQAKPLSLESQTIEFVKGKPLLLLKWVGSD 81

Query: 101 PSLPSILLNSHMDSVPAEPSKWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLR 160
           P+LP+ LLNSH D VP E SKW H P  A     G I+ARGSQD KC+ +QYLEAIR L+
Sbjct: 82  PTLPAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHGDIYARGSQDMKCVGMQYLEAIRKLQ 141

Query: 161 NRDFVPVRTIHISYVPDEEIGGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYA 220
              F P+R++++S+VPDEEIGG DGA KF +S+ FK LN+  ++DEG  SP + +RVFY 
Sbjct: 142 ASGFKPLRSVYLSFVPDEEIGGHDGAEKFAESQLFKSLNIAIVLDEGLPSPTESYRVFYG 201

Query: 221 DRSPWSLIIKAKGSPGHGSRMYDNSAMENLMKSVEIMTRFRESQFEIVKAGEAANSEVIS 280
           +RSPW L+IKAKG PGHG+++YDNSAMENL+KS+E + RFR SQF+++KAG  A  +V+S
Sbjct: 202 ERSPWWLVIKAKGPPGHGAKLYDNSAMENLLKSIESIRRFRASQFDLLKAGGIAEGDVVS 261

Query: 281 VNPVFVNAGTPSPTGFVMNMQPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPARRNMTYQ 340
           VN  F+ AGTPSPTGFVMN+QPSEAEAGFD+R+PP+ D +A+ RR+ EEWAPA RNM+++
Sbjct: 262 VNMAFLKAGTPSPTGFVMNLQPSEAEAGFDIRVPPSVDAEALERRLVEEWAPAARNMSFE 321

Query: 341 ILEKGPIRDFLGSPLMTMTNASNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRI 400
             +K       G   +T  + SNPWW + + A+ +AGG+ SKPEI   +TDAR+ R+  +
Sbjct: 322 FKQK-----LTGKQFLTAADDSNPWWGLLENAVKEAGGRTSKPEIFPASTDARYFRKAGV 381

Query: 401 PVLGFSPMINTPILLHDHNEFLSDSVFIRGIKVYESLISALSSFQEDVSSQ 452
           P  GFSP+ NTP LLHDHNE+L  + +++GI+VY S+I A +S++    S+
Sbjct: 382 PAFGFSPISNTPSLLHDHNEYLGKAEYLKGIEVYVSIIKAYASYESKSGSR 427

[Close]

BLAST of CSPI01G01600 vs. TAIR 10
Match: AT4G38220.2 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 494.2 bits (1271), Expect = 1.1e-139
Identity = 229/412 (55.58%), Postives = 306/412 (74.27%), Query Frame = 0


Query: 41  DSPVSRFQRYLRIKTAHPDPDYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVTWYGSD 100
           D+ VSRFQ YLRI T  P+P+Y  AV F+ SQA+ + L +Q +EFV GKPLLL+ W GSD
Sbjct: 22  DAIVSRFQEYLRINTVQPNPEYYKAVDFIISQAKPLSLESQTIEFVKGKPLLLLKWVGSD 81

Query: 101 PSLPSILLNSHMDSVPAEPSKWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLR 160
           P+LP+ LLNSH D VP E SKW H P  A     G I+ARGSQD KC+ +QYLEAIR L+
Sbjct: 82  PTLPAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHGDIYARGSQDMKCVGMQYLEAIRKLQ 141

Query: 161 NRDFVPVRTIHISYVPDEEIGGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYA 220
              F P+R++++S+VPDEEIGG DGA KF +S+ FK LN+  ++DEG  SP + +RVFY 
Sbjct: 142 ASGFKPLRSVYLSFVPDEEIGGHDGAEKFAESQLFKSLNIAIVLDEGLPSPTESYRVFYG 201

Query: 221 DRSPWSLIIKAKGSPGHGSRMYDNSAMENLMKSVEIMTRFRESQFEIVKAGEAANSEVIS 280
           +RSPW L+IKAKG PGHG+++YDNSAMENL+KS+E + RFR SQF+++KAG  A  +V+S
Sbjct: 202 ERSPWWLVIKAKGPPGHGAKLYDNSAMENLLKSIESIRRFRASQFDLLKAGGIAEGDVVS 261

Query: 281 VNPVFVNAGTPSPTGFVMNMQPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPARRNMTYQ 340
           VN  F+ AGTPSPTGFVMN+QPSEAEAGFD+R+PP+ D +A+ RR+ EEWAPA RNM+++
Sbjct: 262 VNMAFLKAGTPSPTGFVMNLQPSEAEAGFDIRVPPSVDAEALERRLVEEWAPAARNMSFE 321

Query: 341 ILEKGPIRDFL-GSPLMTMTNASNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMR 400
           +   G  +  L G   +T  + SNPWW + + A+ +AGG+ SKPEI   +TDAR+ R+  
Sbjct: 322 L---GQFKQKLTGKQFLTAADDSNPWWGLLENAVKEAGGRTSKPEIFPASTDARYFRKAG 381

Query: 401 IPVLGFSPMINTPILLHDHNEFLSDSVFIRGIKVYESLISALSSFQEDVSSQ 452
           +P  GFSP+ NTP LLHDHNE+L  + +++GI+VY S+I A +S++    S+
Sbjct: 382 VPAFGFSPISNTPSLLHDHNEYLGKAEYLKGIEVYVSIIKAYASYESKSGSR 430

The following BLAST results are available for this feature:

BLAST of CSPI01G01600 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q99JW2	9.3e-91	42.54	Aminoacylase-1 OS=Mus musculus OX=10090 GN=Acy1 PE=1 SV=1	[more]
P37111	1.3e-89	42.00	Aminoacylase-1 OS=Sus scrofa OX=9823 GN=ACY1 PE=1 SV=2	[more]
Q6AYS7	1.5e-88	42.16	Aminoacylase-1A OS=Rattus norvegicus OX=10116 GN=Acy1a PE=1 SV=1	[more]
Q6PTT0	1.5e-88	41.67	Aminoacylase-1B OS=Rattus norvegicus OX=10116 GN=Acy1b PE=1 SV=1	[more]
Q5RFB0	4.3e-88	42.40	Aminoacylase-1 OS=Pongo abelii OX=9601 GN=ACY1 PE=2 SV=2	[more]

BLAST of CSPI01G01600 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LRI1	1.7e-257	99.33	M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G005600 P...	[more]
A0A5D3BIQ8	6.9e-246	94.46	Aminoacylase-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00191...	[more]
A0A5A7URV3	9.0e-246	94.24	Aminoacylase-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G00182...	[more]
A0A6J1JRT3	4.5e-221	84.41	aminoacylase-1 OS=Cucurbita maxima OX=3661 GN=LOC111487760 PE=4 SV=1	[more]
A0A6J1HLT6	1.7e-220	84.41	aminoacylase-1 OS=Cucurbita moschata OX=3662 GN=LOC111464784 PE=4 SV=1	[more]

BLAST of CSPI01G01600 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN63599.1	3.6e-257	99.33	hypothetical protein Csa_013948 [Cucumis sativus]	[more]
TYJ98571.1	1.4e-245	94.46	aminoacylase-1 [Cucumis melo var. makuwa]	[more]
KAA0057884.1	1.9e-245	94.24	aminoacylase-1 [Cucumis melo var. makuwa]	[more]
XP_038879728.1	2.5e-234	89.58	aminoacylase-1-like [Benincasa hispida]	[more]
KAG6602086.1	5.4e-221	84.86	Aminoacylase-1, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of CSPI01G01600 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G44820.1	1.2e-178	67.83	Peptidase M20/M25/M40 family protein	[more]
AT1G44180.1	4.3e-176	67.76	Peptidase M20/M25/M40 family protein	[more]
AT4G38220.1	6.5e-140	55.47	Peptidase M20/M25/M40 family protein	[more]
AT4G38220.2	1.1e-139	55.58	Peptidase M20/M25/M40 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PIRSF	PIRSF036696	ACY-1	coord: 33..446 e-value: 8.0E-169 score: 559.3
None	No IPR available	GENE3D	3.40.630.10	Zn peptidases	coord: 34..225 e-value: 3.5E-56 score: 191.8
None	No IPR available	GENE3D	1.10.150.900		coord: 363..444 e-value: 1.5E-27 score: 97.1
None	No IPR available	GENE3D	3.30.70.360		coord: 226..336 e-value: 7.8E-10 score: 40.6
None	No IPR available	PANTHER	PTHR45892	AMINOACYLASE-1	coord: 29..448
None	No IPR available	PANTHER	PTHR45892:SF3	PUTATIVE-RELATED	coord: 29..448
None	No IPR available	CDD	cd05646	M20_AcylaseI_like	coord: 41..442 e-value: 0.0 score: 627.377
None	No IPR available	SUPERFAMILY	53187	Zn-dependent exopeptidases	coord: 39..441
IPR002933	Peptidase M20	PFAM	PF01546	Peptidase_M20	coord: 107..439 e-value: 4.1E-35 score: 121.9
IPR011650	Peptidase M20, dimerisation domain	PFAM	PF07687	M20_dimer	coord: 223..328 e-value: 9.6E-7 score: 28.7
IPR010159	N-acyl-L-amino-acid amidohydrolase	TIGRFAM	TIGR01880	TIGR01880	coord: 39..444 e-value: 1.3E-180 score: 598.2
IPR036264	Bacterial exopeptidase dimerisation domain	SUPERFAMILY	55031	Bacterial exopeptidase dimerisation domain	coord: 225..333

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G01600.1	CSPI01G01600.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006520	cellular amino acid metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004046	aminoacylase activity
molecular_function	GO:0016787	hydrolase activity