CSPI01G01320 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G01320
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Diphthine methyltransferase
Location	Chr1: 851064 .. 853590 (+)
RNA-Seq Expression	CSPI01G01320
Synteny	CSPI01G01320

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAATTGTGACAAGCGTTAGGCTTAATTTTAAAACTCATTAAAGTTTAAGGGTTTAATTTATAAGATTGTAAGTTTAATTGTGTAATTTCAAGTAGAATCAGCTCCGCCGCTCGTTTCAACTACCCGCCACGGAATCATTGCCTTTCGTCAACATTCGCCGATGGTCTCTGCGTCGTGATTGTTGTCACTGGGTAGTGCCGCTTTTCACTCAGTTCATGTCGTGGGGACTGGGCCATTTTCAATCAAAGTAAATTATAGGTACCGTTTTGTGGGTAGTTTCAATCTGAATTGGTTATTTTTTTGTGCGGTTGACACGAGACCTCCTATTTGGAGGTTGGAATCAATGAGATTGTGGATTGAGACGTTCTGTTCTTGTTTGTATTGTATGGAAAGTTCGATCCGCTGCTGTGGCTTACTTCAAATTCAGATGTATTGAAGTGACCTTTAAATGTGTGTTACTCTCTCTAATTTGTATGGCTGGAGAACATTTGAAAATTAGTTTTCTATTTTTTTAAAAAATTGACTTGCTATTCTTACAATTGTAAAATGTTTTTGCTATTTTTTTGCCAAATGAAAGGTCGAAATGCGTTTCTTGAAGTTCAGTGCCTGCGTGCTTGTTGTTGTTTCTCTTTTACTATTATCATTATTTAATGTCACACGAGGTGTTCGACAAAAGGCCCCACTGACAATGAGTGTTTTTTTTCTTCTAACAAAACTTTTGTGTTGCGATTCAGAAATGGATGTCGCTCACTGCACTCTGGACGGCAATGCTGATGGAGTGGAGTTTTGCCCTCACAGTTCATTTAGTAACGTTCTTGCTGCTTCCACTTATACACTACAAGAGGGTGAGCAGCCCAGTCGTTCTGGAAGCCTATCAATCTTCAATATCAATGCAGATATTGGCTCTTTGGAGCTGCTTCATCGTGTGGAAACGGCGGGGATCTTTGATATAAAGTGGAGCTCTGTTGGTCAGACCGCAAGTCCTCTGCTTGCTCAAGCAGATGCTGATGGCTGTCTCAGAATTCATGCGCTTGAGTGCTCTTCAAATGGTGTAGAAGGTATCGAAATGGGAAACTTTTTTGTCATCGAAATTGGAAACTTTTTTGTCATATTTTTTGGAAATATATGTGGGAATGGAGATGTTTAGTGTACTAGATTATAGATGCTATTTGAGACTGAGTTAAACACAAGACACTGATGACCGGAGAATTAGTCTGTCTTTCCTCTAACAACTACAGACTTCAAGTTTTGCCATTCATTCTATCCCATAAAAATTATGCTGGAATGTATGTAATCAAAAGCTGGTTGGTTGTGGTTAATATAATTATATCACTGAGTTCTTTAAATTGATCAACGAATGAGTGTGAGCTAATGAATTTTCATCAAGTATGAAGTATGAACTCCAAAGAAAGTGATAGGTGCAAACAAAGTCCCTCACTGGTTAGAAATGGAATTGATTAAGGGTATATAAATAAGGACAACTATTTCTATTGGTATAAGGTATTTCTTTTGACTCAAATTACCTTTCAATTTTCATGACTGCAAGACTTTCACCTTTAGATTGAAGTATATAGCTTCCAAGCTTCTGCCTCTTTTTTTACTATTTAAGTTCTTGACGGTCTCTTCTTGGTGCAGGAGAATATTGTTTAAAAGATAAATGTGGCAGCAAAATTTCGTCCTCAATGTGTCTTTGCCTGGATTGGAATACTTCGGCCTCATCCATCTCTGTCGGGCATTCAGATGGTTCTATCTCAATACTCTCTGCAGGAGAATCCCATTTAGAAGTCCAAAACCAATGGAAAGCTCACGAGTTTGAGCTTTGGGCAACCTCTTTTGACACACACCAACCTCATTTAGTGTACACTGGCTCAGATGACTGCAAATTTAGCTGTTGGGATTTGCGAGACACTCCTTCCAAAGTTTTTCATAACACCAAAGTCCACAAAATGGGTGTTTGTTGCATTGCCAAAATTCCCAATGACCCTTACAAGTTACTCACTGGAAGCTACGATGAAAACCTGAGAGTGTGGGACATACGAGCAATCTCAAAGCCCATAAATGAAACTTCAATCTGCTTGGGCGGTGGCGTCTGGAAAATCAAGCCCCACCCTTCAGTTTCTGACGTTGTTCTGGCTGCTTGTATGCACAGTGGATTTGCAATTGTTAGAGTAAAAGACGATGGTGCAGAAGTAGTTGAAACTTATGCCAAGCACAACTCTCTTGCTTATGGAGCAGATTGGCAAAGAGGAGATTCGTCTCAAGAAGATAAAAGGAAAAGGTATGCTGTTGCAACTTGTTCATTTTATGATAAGCTTCTCAGGATATGGATACCAGAAACCGATATTATCACTTGATTGCTTCACACACATGTTAAATTGGATGTTTTTCTTCAAGCATGTTGATTTTCTTGATAAATTTATCTGTTTGTTCTTACCTTGTTCGGTATTGTATTGGAGGATTTTATTTCGTTTTATTGCATACATGTTCTTGTGCAGACGTTGACATGTTCCCTTTGTAAATTATTTGG

mRNA sequence

CAATTGTGACAAGCGTTAGGCTTAATTTTAAAACTCATTAAAGTTTAAGGGTTTAATTTATAAGATTGTAAGTTTAATTGTGTAATTTCAAGTAGAATCAGCTCCGCCGCTCGTTTCAACTACCCGCCACGGAATCATTGCCTTTCGTCAACATTCGCCGATGGTCTCTGCGTCGTGATTGTTGTCACTGGGTAGTGCCGCTTTTCACTCAGTTCATGTCGTGGGGACTGGGCCATTTTCAATCAAAGTAAATTATAGAAATGGATGTCGCTCACTGCACTCTGGACGGCAATGCTGATGGAGTGGAGTTTTGCCCTCACAGTTCATTTAGTAACGTTCTTGCTGCTTCCACTTATACACTACAAGAGGGTGAGCAGCCCAGTCGTTCTGGAAGCCTATCAATCTTCAATATCAATGCAGATATTGGCTCTTTGGAGCTGCTTCATCGTGTGGAAACGGCGGGGATCTTTGATATAAAGTGGAGCTCTGTTGGTCAGACCGCAAGTCCTCTGCTTGCTCAAGCAGATGCTGATGGCTGTCTCAGAATTCATGCGCTTGAGTGCTCTTCAAATGGTGTAGAAGGAGAATATTGTTTAAAAGATAAATGTGGCAGCAAAATTTCGTCCTCAATGTGTCTTTGCCTGGATTGGAATACTTCGGCCTCATCCATCTCTGTCGGGCATTCAGATGGTTCTATCTCAATACTCTCTGCAGGAGAATCCCATTTAGAAGTCCAAAACCAATGGAAAGCTCACGAGTTTGAGCTTTGGGCAACCTCTTTTGACACACACCAACCTCATTTAGTGTACACTGGCTCAGATGACTGCAAATTTAGCTGTTGGGATTTGCGAGACACTCCTTCCAAAGTTTTTCATAACACCAAAGTCCACAAAATGGGTGTTTGTTGCATTGCCAAAATTCCCAATGACCCTTACAAGTTACTCACTGGAAGCTACGATGAAAACCTGAGAGTGTGGGACATACGAGCAATCTCAAAGCCCATAAATGAAACTTCAATCTGCTTGGGCGGTGGCGTCTGGAAAATCAAGCCCCACCCTTCAGTTTCTGACGTTGTTCTGGCTGCTTGTATGCACAGTGGATTTGCAATTGTTAGAGTAAAAGACGATGGTGCAGAAGTAGTTGAAACTTATGCCAAGCACAACTCTCTTGCTTATGGAGCAGATTGGCAAAGAGGAGATTCGTCTCAAGAAGATAAAAGGAAAAGGTATGCTGTTGCAACTTGTTCATTTTATGATAAGCTTCTCAGGATATGGATACCAGAAACCGATATTATCACTTGATTGCTTCACACACATGTTAAATTGGATGTTTTTCTTCAAGCATGTTGATTTTCTTGATAAATTTATCTGTTTGTTCTTACCTTGTTCGGTATTGTATTGGAGGATTTTATTTCGTTTTATTGCATACATGTTCTTGTGCAGACGTTGACATGTTCCCTTTGTAAATTATTTGG

Coding sequence (CDS)

ATGGATGTCGCTCACTGCACTCTGGACGGCAATGCTGATGGAGTGGAGTTTTGCCCTCACAGTTCATTTAGTAACGTTCTTGCTGCTTCCACTTATACACTACAAGAGGGTGAGCAGCCCAGTCGTTCTGGAAGCCTATCAATCTTCAATATCAATGCAGATATTGGCTCTTTGGAGCTGCTTCATCGTGTGGAAACGGCGGGGATCTTTGATATAAAGTGGAGCTCTGTTGGTCAGACCGCAAGTCCTCTGCTTGCTCAAGCAGATGCTGATGGCTGTCTCAGAATTCATGCGCTTGAGTGCTCTTCAAATGGTGTAGAAGGAGAATATTGTTTAAAAGATAAATGTGGCAGCAAAATTTCGTCCTCAATGTGTCTTTGCCTGGATTGGAATACTTCGGCCTCATCCATCTCTGTCGGGCATTCAGATGGTTCTATCTCAATACTCTCTGCAGGAGAATCCCATTTAGAAGTCCAAAACCAATGGAAAGCTCACGAGTTTGAGCTTTGGGCAACCTCTTTTGACACACACCAACCTCATTTAGTGTACACTGGCTCAGATGACTGCAAATTTAGCTGTTGGGATTTGCGAGACACTCCTTCCAAAGTTTTTCATAACACCAAAGTCCACAAAATGGGTGTTTGTTGCATTGCCAAAATTCCCAATGACCCTTACAAGTTACTCACTGGAAGCTACGATGAAAACCTGAGAGTGTGGGACATACGAGCAATCTCAAAGCCCATAAATGAAACTTCAATCTGCTTGGGCGGTGGCGTCTGGAAAATCAAGCCCCACCCTTCAGTTTCTGACGTTGTTCTGGCTGCTTGTATGCACAGTGGATTTGCAATTGTTAGAGTAAAAGACGATGGTGCAGAAGTAGTTGAAACTTATGCCAAGCACAACTCTCTTGCTTATGGAGCAGATTGGCAAAGAGGAGATTCGTCTCAAGAAGATAAAAGGAAAAGGTATGCTGTTGCAACTTGTTCATTTTATGATAAGCTTCTCAGGATATGGATACCAGAAACCGATATTATCACTTGA

Protein sequence

MDVAHCTLDGNADGVEFCPHSSFSNVLAASTYTLQEGEQPSRSGSLSIFNINADIGSLELLHRVETAGIFDIKWSSVGQTASPLLAQADADGCLRIHALECSSNGVEGEYCLKDKCGSKISSSMCLCLDWNTSASSISVGHSDGSISILSAGESHLEVQNQWKAHEFELWATSFDTHQPHLVYTGSDDCKFSCWDLRDTPSKVFHNTKVHKMGVCCIAKIPNDPYKLLTGSYDENLRVWDIRAISKPINETSICLGGGVWKIKPHPSVSDVVLAACMHSGFAIVRVKDDGAEVVETYAKHNSLAYGADWQRGDSSQEDKRKRYAVATCSFYDKLLRIWIPETDIIT*

Homology

[Close]

BLAST of CSPI01G01320 vs. ExPASy Swiss-Prot
Match: Q55C80 (Diphthine methyltransferase homolog OS=Dictyostelium discoideum OX=44689 GN=wdr85 PE=3 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 4.6e-45
Identity = 119/375 (31.73%), Postives = 185/375 (49.33%), Query Frame = 0


Query: 8   LDGNADGVEFCPHSSFSNVLAASTYTLQEGE---QPSRSGSLSIFNI------------N 67
           LD  +D VEF P +  +N+    TY +++G+   +  R G L +F I            N
Sbjct: 11  LDYTSDSVEFYPFN--NNIFVCGTYEIEKGDTEYKERRKGKLYLFEIEEEQQQKENDNNN 70

Query: 68  ADIGSLELLHRVE----TAGIFDIKWSSVGQTASPLLAQADADGCLRIHALECSSNGVEG 127
            +  + +L   ++     +GI D+KW++       +L    + G L I+  +      E 
Sbjct: 71  ENNNNNKLFKEIQNINFNSGILDMKWNN---NKDRILGVVMSKGELNIYQYD------EV 130

Query: 128 EYCLKDKCGSKISSS---MCLCLDWNTSASSISVGHSDGSISILSAGESHLEV--QNQWK 187
           E  L+ K  ++IS S   + L LDWN S   +    SDG+I +    + + +V  + +WK
Sbjct: 131 EKKLELKSSTEISLSNDILSLSLDWNKSGDKLICSFSDGNIGLFKVTKDYSKVTEEKRWK 190

Query: 188 AHEFELWATSFDTHQPHLVYTGSDDCKFSCWDLRD-----------TPSKVFHNTKVHKM 247
           AH++E W  +F+ H   +V++G DDCKF  WDL              P+  F  TK   M
Sbjct: 191 AHDYEAWICAFNYHDESIVFSGGDDCKFKIWDLNQLLNHNDDDIGIPPTPKF--TKRCDM 250

Query: 248 GVCCIAKIPNDPYKLLTGSYDENLRVWDIRAISKPINETSICLGGGVWKIKPHPSVSDVV 307
           GV  I   P     +  GSYDE LR+WD++++ +PI  T   LGGG+W+IK HP   + +
Sbjct: 251 GVTSIHCHPTIENLIAVGSYDECLRIWDLKSLKQPIITTD-SLGGGIWRIKWHPFQKNKL 310

Query: 308 LAACMHSGFAIVRVKDDG------AEVVETY-AKHNSLAYGADWQRGDSSQEDKRKRYAV 341
           + ACM  GF I+    +        ++ ++Y   H S+AYG DW    S  ++   +  +
Sbjct: 311 VTACMGGGFHILSTDPENINDFSTLQIEQSYNGPHKSIAYGVDW----SFNKNNFDKQFI 367

[Close]

BLAST of CSPI01G01320 vs. ExPASy Swiss-Prot
Match: Q9BTV6 (Diphthine methyltransferase OS=Homo sapiens OX=9606 GN=DPH7 PE=1 SV=2)

HSP 1 Score: 181.8 bits (460), Expect = 1.3e-44
Identity = 115/340 (33.82%), Postives = 162/340 (47.65%), Query Frame = 0


Query: 12  ADGVEFCPHSSFSNVLAASTYTLQEGEQ----PSRSGSLSI--------------FNINA 71
           AD VE+CP     ++LA  TY L+  E     P   G + +              FN N 
Sbjct: 16  ADSVEWCPLQGCRHLLACGTYQLRRPEDRPAGPQNKGGMEVKEPQVRLGRLFLYSFNDNN 75

Query: 72  DIGSLELLHRVETAGIFDIKWSSVGQTASPLLAQADADGCLRI---------HALECSSN 131
            I  L  + R +T+ I D+KW  +      LL  ADA G +++         H LE  S+
Sbjct: 76  SIHPLVEVQRKDTSAILDMKWCHIPVAGHALLGLADASGSIQLLRLVESEKSHVLEPLSS 135

Query: 132 GVEGEYCLKDKCGSKISSSMCLCLDWNTSASSISVGHSDGSISILSAGESHLEVQNQ--- 191
               E CL             L LDW+T  +  +       IS  S G+ HL + N+   
Sbjct: 136 LALEEQCL------------ALSLDWSTGKTGRAGDQPLKIISSDSTGQLHLLMVNETRP 195

Query: 192 -------WKAHEFELWATSFDTHQPHLVYTGSDDCKFSCWDLRDTPSKVFHNTKVHKMGV 251
                  W+AH+FE W  +F+   P +VY+G DD     WD R  P K    +K H MGV
Sbjct: 196 RLQKVASWQAHQFEAWIAAFNYWHPEIVYSGGDDGLLRGWDTR-VPGKFLFTSKRHTMGV 255

Query: 252 CCIAKIPNDPYKLLTGSYDENLRVWDIRAISKPINETSICLGGGVWKIKPHPSVSDVVLA 310
           C I   P+  + L TGSYDE++ +WD R + +P+ +T +   GGVW+IK HP    ++LA
Sbjct: 256 CSIQSSPHREHILATGSYDEHILLWDTRNMKQPLADTPV--QGGVWRIKWHPFHHHLLLA 315

[Close]

BLAST of CSPI01G01320 vs. ExPASy Swiss-Prot
Match: Q9CYU6 (Diphthine methyltransferase OS=Mus musculus OX=10090 GN=Dph7 PE=2 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 1.9e-43
Identity = 111/326 (34.05%), Postives = 167/326 (51.23%), Query Frame = 0


Query: 12  ADGVEFCPHSSFSNVLAASTYTLQ--------EGEQPS-RSGSLSIFNINADIGSLELL- 71
           AD VE+CP   + ++LA  TY L+        +G +P  R G L +F+ +    +  LL 
Sbjct: 21  ADSVEWCPVEGYQHLLACGTYQLRAPRDQPALDGSEPQVRLGRLYLFSFSEHNTAKPLLE 80

Query: 72  -HRVETAGIFDIKWSSVGQTASPLLAQADADGCL-RIHALECSSNGVEGEYCLKDKCGSK 131
             R +++ + D+KW  +  +   LL  A+A G +  +  +EC +N     Y L+      
Sbjct: 81  VQRRDSSAVLDMKWCHIPVSGHVLLGLANASGSIGLLRLMECENN----SYTLQPISSLA 140

Query: 132 ISSS-MCLCLDWNTSAS--------SISVGHSDGSISILSAGESHLEVQ--NQWKAHEFE 191
           +  + + L +DW+T  S         I    S G + +L   E   E+Q    W AH FE
Sbjct: 141 LDENCLSLSMDWSTGKSVRAREQPLKIISSDSKGQLHLLMVNEGTAELQLVASWPAHHFE 200

Query: 192 LWATSFDTHQPHLVYTGSDDCKFSCWDLRDTPSKVFHNTKVHKMGVCCIAKIPNDPYKLL 251
            W  +F+  Q  LVY+G DDC    WD R   + VF  +K H MGVC I   P+  + L 
Sbjct: 201 AWIAAFNYWQTELVYSGGDDCLLRGWDTRMLGTPVF-TSKRHCMGVCSIQSSPHQEHILA 260

Query: 252 TGSYDENLRVWDIRAISKPINETSICLGGGVWKIKPHPSVSDVVLAACMHSGFAIVRVKD 310
           TGSYDE++ +WD R I +P+ +  +   GGVW++K HP    ++LAACMH+GF I+  + 
Sbjct: 261 TGSYDEHVLLWDTRNIRQPLADVPV--QGGVWRLKWHPVHHHLLLAACMHNGFKILNCQK 320

[Close]

BLAST of CSPI01G01320 vs. ExPASy Swiss-Prot
Match: P38332 (Diphthine methyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RRT2 PE=1 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 2.4e-25
Identity = 71/213 (33.33%), Postives = 105/213 (49.30%), Query Frame = 0


Query: 155 HLEVQNQWKAHEFELWATSFDTHQP--HLVYTGSDDCKFSCWDLRDTPSKVFHNTKVHKM 214
           H+E     K HE E W   F + QP   +V+TG DD +    DLR +   ++ N ++H  
Sbjct: 186 HVESGQFLKPHELECWTAEFGSLQPFQDVVFTGGDDSRIMAHDLR-SKEFIWSNNRIHDA 245

Query: 215 GVCCI-AKIP----NDPYKLLTGSYDENLRVWDIRAISKPI---------NETSICLGGG 274
           GV  I    P    N P  ++TGSYD+N+R  D+R + + I         N+ +  LGGG
Sbjct: 246 GVVSIKCSQPNFRNNKPTSIITGSYDDNIRSLDLRMMGESIFPGANVPTVNKLACDLGGG 305

Query: 275 VWKI--------KPHPSVSDVVLAACMHSGFAIVRVKDDGAEV--VETYAK--HNSLAYG 334
           VW+         + H + SD +L  CM++G  +V + D+  E   ++ Y K  H+S+ YG
Sbjct: 306 VWRFVESPIDQEQSHHNGSDRLLVCCMYNGAKVVTMNDNSDEYFQIQHYLKKGHDSMCYG 365

Query: 335 ADWQRGDSSQEDKRKRYAVATCSFYDKLLRIWI 340
            DW               +ATCSFYD  L+ WI
Sbjct: 366 GDWSNS-----------LIATCSFYDNSLQTWI 386

[Close]

BLAST of CSPI01G01320 vs. ExPASy Swiss-Prot
Match: P43254 (E3 ubiquitin-protein ligase COP1 OS=Arabidopsis thaliana OX=3702 GN=COP1 PE=1 SV=2)

HSP 1 Score: 67.0 bits (162), Expect = 4.8e-10
Identity = 43/171 (25.15%), Postives = 79/171 (46.20%), Query Frame = 0


Query: 84  LLAQADADGCLRIHALECSSNGVEGEYC--LKDKCGSKISSSMCLCLDWNT-SASSISVG 143
           L A A    C+++       N      C  ++    SK+S     CL WN    + I+  
Sbjct: 385 LFATAGVSRCIKVFDFSSVVNEPADMQCPIVEMSTRSKLS-----CLSWNKHEKNHIASS 444

Query: 144 HSDGSISI--LSAGESHLEVQNQWKAHEFELWATSFDTHQPHLVYTGSDDCKFSCWDLRD 203
             +G +++  ++  +S +E    ++ HE   W+  F   +P ++ +GSDDCK   W  R 
Sbjct: 445 DYEGIVTVWDVTTRQSLME----YEEHEKRAWSVDFSRTEPSMLVSGSDDCKVKVWCTRQ 504

Query: 204 TPSKVFHNTKVHKMGVCCIAKIPNDPYKLLTGSYDENLRVWDIRAISKPIN 250
             S +  + K +   +CC+   P     +  GS D ++  +D+R IS+P++
Sbjct: 505 EASVINIDMKAN---ICCVKYNPGSSNYIAVGSADHHIHYYDLRNISQPLH 543

[Close]

BLAST of CSPI01G01320 vs. ExPASy TrEMBL
Match: A0A0A0LS22 (WD_REPEATS_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G004880 PE=4 SV=1)

HSP 1 Score: 725.3 bits (1871), Expect = 1.2e-205
Identity = 344/346 (99.42%), Postives = 346/346 (100.00%), Query Frame = 0


Query: 1   MDVAHCTLDGNADGVEFCPHSSFSNVLAASTYTLQEGEQPSRSGSLSIFNINADIGSLEL 60
           MDVAHCTLDGNADGVEFCPHSSFSNVLAASTYTLQEGEQPSRSGSLSIFNINADIGSLEL
Sbjct: 1   MDVAHCTLDGNADGVEFCPHSSFSNVLAASTYTLQEGEQPSRSGSLSIFNINADIGSLEL 60

Query: 61  LHRVETAGIFDIKWSSVGQTASPLLAQADADGCLRIHALECSSNGVEGEYCLKDKCGSKI 120
           LHRVETAGIFDIKWSSVGQTASPLLAQADADGCLRIHALECSSNGVEGEYCLK+KCGSKI
Sbjct: 61  LHRVETAGIFDIKWSSVGQTASPLLAQADADGCLRIHALECSSNGVEGEYCLKEKCGSKI 120

Query: 121 SSSMCLCLDWNTSASSISVGHSDGSISILSAGESHLEVQNQWKAHEFELWATSFDTHQPH 180
           SSSMCLCLDWNTSASSISVGHSDGSISILSAGESHLEVQNQWKAHEFELWATSFDTHQPH
Sbjct: 121 SSSMCLCLDWNTSASSISVGHSDGSISILSAGESHLEVQNQWKAHEFELWATSFDTHQPH 180

Query: 181 LVYTGSDDCKFSCWDLRDTPSKVFHNTKVHKMGVCCIAKIPNDPYKLLTGSYDENLRVWD 240
           LVYTGSDDCKFSCWDLRDTPSKVFHNTKVHKMGVCCIAKIPNDPYKLLTGSYDENLRVWD
Sbjct: 181 LVYTGSDDCKFSCWDLRDTPSKVFHNTKVHKMGVCCIAKIPNDPYKLLTGSYDENLRVWD 240

Query: 241 IRAISKPINETSICLGGGVWKIKPHPSVSDVVLAACMHSGFAIVRVKDDGAEVVETYAKH 300
           IRAISKPINETSICLGGGVWKIKPHPSVSDVVLAACMH+GFAIVRVKDDGAEVVETYAKH
Sbjct: 241 IRAISKPINETSICLGGGVWKIKPHPSVSDVVLAACMHNGFAIVRVKDDGAEVVETYAKH 300

Query: 301 NSLAYGADWQRGDSSQEDKRKRYAVATCSFYDKLLRIWIPETDIIT 347
           NSLAYGADWQRGDSSQEDKRKRYAVATCSFYDKLLRIWIPETDIIT
Sbjct: 301 NSLAYGADWQRGDSSQEDKRKRYAVATCSFYDKLLRIWIPETDIIT 346

[Close]

BLAST of CSPI01G01320 vs. ExPASy TrEMBL
Match: A0A1S3BVC6 (diphthine methyltransferase OS=Cucumis melo OX=3656 GN=LOC103493910 PE=4 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 1.3e-199
Identity = 336/346 (97.11%), Postives = 342/346 (98.84%), Query Frame = 0


Query: 1   MDVAHCTLDGNADGVEFCPHSSFSNVLAASTYTLQEGEQPSRSGSLSIFNINADIGSLEL 60
           MDVAHCTLDGNADGVEFCPHSSFSNVLAASTYTLQEGEQPSRSGSLSIFN+NADIGSLEL
Sbjct: 1   MDVAHCTLDGNADGVEFCPHSSFSNVLAASTYTLQEGEQPSRSGSLSIFNVNADIGSLEL 60

Query: 61  LHRVETAGIFDIKWSSVGQTASPLLAQADADGCLRIHALECSSNGVEGEYCLKDKCGSKI 120
           LHRVETAGIFDIKWS VGQTASPLLAQADADGCLRIHALECSSNGVEGEY LK+KCGSKI
Sbjct: 61  LHRVETAGIFDIKWSPVGQTASPLLAQADADGCLRIHALECSSNGVEGEY-LKEKCGSKI 120

Query: 121 SSSMCLCLDWNTSASSISVGHSDGSISILSAGESHLEVQNQWKAHEFELWATSFDTHQPH 180
           SSSMCLCLDWNTSASSISVGHSDGSISILSAGESHLEVQNQWKAHEFELWAT+FDTHQPH
Sbjct: 121 SSSMCLCLDWNTSASSISVGHSDGSISILSAGESHLEVQNQWKAHEFELWATAFDTHQPH 180

Query: 181 LVYTGSDDCKFSCWDLRDTPSKVFHNTKVHKMGVCCIAKIPNDPYKLLTGSYDENLRVWD 240
           LVYTGSDDCKFSCWDLRDTPSKVFHNTKVHKMGVCCIAKIPNDPYKLLTGSYDENLRVWD
Sbjct: 181 LVYTGSDDCKFSCWDLRDTPSKVFHNTKVHKMGVCCIAKIPNDPYKLLTGSYDENLRVWD 240

Query: 241 IRAISKPINETSICLGGGVWKIKPHPSVSDVVLAACMHSGFAIVRVKDDGAEVVETYAKH 300
           IRAISKPINETSICLGGGVWKIKPHPSV DVVLAACMH+GFAIVRVK+DGAEVVETYAKH
Sbjct: 241 IRAISKPINETSICLGGGVWKIKPHPSVFDVVLAACMHNGFAIVRVKEDGAEVVETYAKH 300

Query: 301 NSLAYGADWQRGDSSQEDKRKRYAVATCSFYDKLLRIWIPETDIIT 347
           +SLAYGADWQR DSSQEDKRKRYAVATCSFYDKLLRIWIPETDIIT
Sbjct: 301 SSLAYGADWQREDSSQEDKRKRYAVATCSFYDKLLRIWIPETDIIT 345

[Close]

BLAST of CSPI01G01320 vs. ExPASy TrEMBL
Match: A0A5A7UUM5 (Diphthine methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001730 PE=4 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 1.3e-199
Identity = 336/346 (97.11%), Postives = 342/346 (98.84%), Query Frame = 0


Query: 1   MDVAHCTLDGNADGVEFCPHSSFSNVLAASTYTLQEGEQPSRSGSLSIFNINADIGSLEL 60
           MDVAHCTLDGNADGVEFCPHSSFSNVLAASTYTLQEGEQPSRSGSLSIFN+NADIGSLEL
Sbjct: 1   MDVAHCTLDGNADGVEFCPHSSFSNVLAASTYTLQEGEQPSRSGSLSIFNVNADIGSLEL 60

Query: 61  LHRVETAGIFDIKWSSVGQTASPLLAQADADGCLRIHALECSSNGVEGEYCLKDKCGSKI 120
           LHRVETAGIFDIKWS VGQTASPLLAQADADGCLRIHALECSSNGVEGEY LK+KCGSKI
Sbjct: 61  LHRVETAGIFDIKWSPVGQTASPLLAQADADGCLRIHALECSSNGVEGEY-LKEKCGSKI 120

Query: 121 SSSMCLCLDWNTSASSISVGHSDGSISILSAGESHLEVQNQWKAHEFELWATSFDTHQPH 180
           SSSMCLCLDWNTSASSISVGHSDGSISILSAGESHLEVQNQWKAHEFELWAT+FDTHQPH
Sbjct: 121 SSSMCLCLDWNTSASSISVGHSDGSISILSAGESHLEVQNQWKAHEFELWATAFDTHQPH 180

Query: 181 LVYTGSDDCKFSCWDLRDTPSKVFHNTKVHKMGVCCIAKIPNDPYKLLTGSYDENLRVWD 240
           LVYTGSDDCKFSCWDLRDTPSKVFHNTKVHKMGVCCIAKIPNDPYKLLTGSYDENLRVWD
Sbjct: 181 LVYTGSDDCKFSCWDLRDTPSKVFHNTKVHKMGVCCIAKIPNDPYKLLTGSYDENLRVWD 240

Query: 241 IRAISKPINETSICLGGGVWKIKPHPSVSDVVLAACMHSGFAIVRVKDDGAEVVETYAKH 300
           IRAISKPINETSICLGGGVWKIKPHPSV DVVLAACMH+GFAIVRVK+DGAEVVETYAKH
Sbjct: 241 IRAISKPINETSICLGGGVWKIKPHPSVFDVVLAACMHNGFAIVRVKEDGAEVVETYAKH 300

Query: 301 NSLAYGADWQRGDSSQEDKRKRYAVATCSFYDKLLRIWIPETDIIT 347
           +SLAYGADWQR DSSQEDKRKRYAVATCSFYDKLLRIWIPETDIIT
Sbjct: 301 SSLAYGADWQREDSSQEDKRKRYAVATCSFYDKLLRIWIPETDIIT 345

[Close]

BLAST of CSPI01G01320 vs. ExPASy TrEMBL
Match: A0A6J1ERS6 (diphthine methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111435275 PE=4 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 2.2e-183
Identity = 309/345 (89.57%), Postives = 326/345 (94.49%), Query Frame = 0


Query: 1   MDVAHCTLDGNADGVEFCPHSSFSNVLAASTYTLQEGEQPSRSGSLSIFNINADIGSLEL 60
           MDVAHCTLDGNAD VEFCPHSSFSNVLAASTYTL EG+QPSRSGSLSIFN+NAD  SLEL
Sbjct: 16  MDVAHCTLDGNADAVEFCPHSSFSNVLAASTYTLLEGDQPSRSGSLSIFNVNADNCSLEL 75

Query: 61  LHRVETAGIFDIKWSSVGQTASPLLAQADADGCLRIHALECSSNGVEGEYCLKDKCGSKI 120
           LHRV+TAGIFDIKWS VG TASPLLAQADADGC+R+HALE SSNG EG+Y LK++CGSKI
Sbjct: 76  LHRVDTAGIFDIKWSPVGPTASPLLAQADADGCIRVHALEHSSNGGEGQY-LKERCGSKI 135

Query: 121 SSSMCLCLDWNTSASSISVGHSDGSISILSAGESHLEVQNQWKAHEFELWATSFDTHQPH 180
           SSSMCLCLDWN SA+SISVGHSDGSISILSAGESHLEVQNQW+AHEFELWATSFDTHQPH
Sbjct: 136 SSSMCLCLDWNPSATSISVGHSDGSISILSAGESHLEVQNQWEAHEFELWATSFDTHQPH 195

Query: 181 LVYTGSDDCKFSCWDLRDTPSKVFHNTKVHKMGVCCIAKIPNDPYKLLTGSYDENLRVWD 240
           LVYTGSDDCKFSCWDLRDTP+K FHNTK HKMGVCCIAK PNDPYKLLTGSYDE+LRVWD
Sbjct: 196 LVYTGSDDCKFSCWDLRDTPTKAFHNTKAHKMGVCCIAKCPNDPYKLLTGSYDEHLRVWD 255

Query: 241 IRAISKPINETSICLGGGVWKIKPHPSVSDVVLAACMHSGFAIVRVKDDG-AEVVETYAK 300
           IR+ISKP NETSICLGGGVW+IKPHPSV DVVLAACMH+GFAIVRVKDD  AEVVETY K
Sbjct: 256 IRSISKPTNETSICLGGGVWRIKPHPSVFDVVLAACMHNGFAIVRVKDDDTAEVVETYDK 315

Query: 301 HNSLAYGADWQRGDSSQEDKRKRYAVATCSFYDKLLRIWIPETDI 345
           H+SLAYGADWQRGDSS EDKRKRYAVATCSFYD+LLRIWIPETDI
Sbjct: 316 HSSLAYGADWQRGDSSHEDKRKRYAVATCSFYDRLLRIWIPETDI 359

[Close]

BLAST of CSPI01G01320 vs. ExPASy TrEMBL
Match: A0A6J1KQV5 (diphthine methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111497846 PE=4 SV=1)

HSP 1 Score: 642.1 bits (1655), Expect = 1.3e-180
Identity = 302/344 (87.79%), Postives = 321/344 (93.31%), Query Frame = 0


Query: 1   MDVAHCTLDGNADGVEFCPHSSFSNVLAASTYTLQEGEQPSRSGSLSIFNINADIGSLEL 60
           MDVAHCTLDGNAD VEFCPHSSFSNVLAASTYTL EG++PSRSG LSIFN+NAD  SLEL
Sbjct: 16  MDVAHCTLDGNADAVEFCPHSSFSNVLAASTYTLLEGDRPSRSGCLSIFNVNADDCSLEL 75

Query: 61  LHRVETAGIFDIKWSSVGQTASPLLAQADADGCLRIHALECSSNGVEGEYCLKDKCGSKI 120
           LHRV+TAGIFDIKWS VGQT SPLLAQADADGC+R+HALE SSNG EG+Y LK++CGSKI
Sbjct: 76  LHRVDTAGIFDIKWSPVGQTISPLLAQADADGCIRVHALEHSSNGAEGQY-LKERCGSKI 135

Query: 121 SSSMCLCLDWNTSASSISVGHSDGSISILSAGESHLEVQNQWKAHEFELWATSFDTHQPH 180
           SSSMCLCLDWN SA+SISVGHSDGSIS+LSAGES LEVQNQWKAHEFELWATSFD HQPH
Sbjct: 136 SSSMCLCLDWNPSATSISVGHSDGSISLLSAGESRLEVQNQWKAHEFELWATSFDPHQPH 195

Query: 181 LVYTGSDDCKFSCWDLRDTPSKVFHNTKVHKMGVCCIAKIPNDPYKLLTGSYDENLRVWD 240
           LVYTGSDDCKFSCWDLRDTP+K FHNTK HKMGVCCIAK  NDPYKLLTGSYDE+LRVWD
Sbjct: 196 LVYTGSDDCKFSCWDLRDTPTKAFHNTKAHKMGVCCIAKCLNDPYKLLTGSYDEHLRVWD 255

Query: 241 IRAISKPINETSICLGGGVWKIKPHPSVSDVVLAACMHSGFAIVRVKDDGAEVVETYAKH 300
           IR+ISKP NETSICLGGGVW+IKPHPSV DVVLAACMH+GFAIVRVKDD AEVVETY KH
Sbjct: 256 IRSISKPTNETSICLGGGVWRIKPHPSVFDVVLAACMHNGFAIVRVKDDTAEVVETYDKH 315

Query: 301 NSLAYGADWQRGDSSQEDKRKRYAVATCSFYDKLLRIWIPETDI 345
           +SLAYGADWQRGDSS ED+RKRY VATCSFYD+LLRIWIPETDI
Sbjct: 316 SSLAYGADWQRGDSSHEDERKRYTVATCSFYDRLLRIWIPETDI 358

[Close]

BLAST of CSPI01G01320 vs. NCBI nr
Match: XP_011656736.1 (diphthine methyltransferase homolog [Cucumis sativus] >XP_011656781.1 diphthine methyltransferase homolog [Cucumis sativus] >XP_031736197.1 diphthine methyltransferase homolog [Cucumis sativus] >KGN63572.1 hypothetical protein Csa_013548 [Cucumis sativus])

HSP 1 Score: 725.3 bits (1871), Expect = 2.5e-205
Identity = 344/346 (99.42%), Postives = 346/346 (100.00%), Query Frame = 0


Query: 1   MDVAHCTLDGNADGVEFCPHSSFSNVLAASTYTLQEGEQPSRSGSLSIFNINADIGSLEL 60
           MDVAHCTLDGNADGVEFCPHSSFSNVLAASTYTLQEGEQPSRSGSLSIFNINADIGSLEL
Sbjct: 1   MDVAHCTLDGNADGVEFCPHSSFSNVLAASTYTLQEGEQPSRSGSLSIFNINADIGSLEL 60

Query: 61  LHRVETAGIFDIKWSSVGQTASPLLAQADADGCLRIHALECSSNGVEGEYCLKDKCGSKI 120
           LHRVETAGIFDIKWSSVGQTASPLLAQADADGCLRIHALECSSNGVEGEYCLK+KCGSKI
Sbjct: 61  LHRVETAGIFDIKWSSVGQTASPLLAQADADGCLRIHALECSSNGVEGEYCLKEKCGSKI 120

Query: 121 SSSMCLCLDWNTSASSISVGHSDGSISILSAGESHLEVQNQWKAHEFELWATSFDTHQPH 180
           SSSMCLCLDWNTSASSISVGHSDGSISILSAGESHLEVQNQWKAHEFELWATSFDTHQPH
Sbjct: 121 SSSMCLCLDWNTSASSISVGHSDGSISILSAGESHLEVQNQWKAHEFELWATSFDTHQPH 180

Query: 181 LVYTGSDDCKFSCWDLRDTPSKVFHNTKVHKMGVCCIAKIPNDPYKLLTGSYDENLRVWD 240
           LVYTGSDDCKFSCWDLRDTPSKVFHNTKVHKMGVCCIAKIPNDPYKLLTGSYDENLRVWD
Sbjct: 181 LVYTGSDDCKFSCWDLRDTPSKVFHNTKVHKMGVCCIAKIPNDPYKLLTGSYDENLRVWD 240

Query: 241 IRAISKPINETSICLGGGVWKIKPHPSVSDVVLAACMHSGFAIVRVKDDGAEVVETYAKH 300
           IRAISKPINETSICLGGGVWKIKPHPSVSDVVLAACMH+GFAIVRVKDDGAEVVETYAKH
Sbjct: 241 IRAISKPINETSICLGGGVWKIKPHPSVSDVVLAACMHNGFAIVRVKDDGAEVVETYAKH 300

Query: 301 NSLAYGADWQRGDSSQEDKRKRYAVATCSFYDKLLRIWIPETDIIT 347
           NSLAYGADWQRGDSSQEDKRKRYAVATCSFYDKLLRIWIPETDIIT
Sbjct: 301 NSLAYGADWQRGDSSQEDKRKRYAVATCSFYDKLLRIWIPETDIIT 346

[Close]

BLAST of CSPI01G01320 vs. NCBI nr
Match: XP_008453090.1 (PREDICTED: diphthine methyltransferase [Cucumis melo] >XP_008453091.1 PREDICTED: diphthine methyltransferase [Cucumis melo] >XP_008453092.1 PREDICTED: diphthine methyltransferase [Cucumis melo] >KAA0057866.1 diphthine methyltransferase [Cucumis melo var. makuwa] >TYJ98556.1 diphthine methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 705.3 bits (1819), Expect = 2.6e-199
Identity = 336/346 (97.11%), Postives = 342/346 (98.84%), Query Frame = 0


Query: 1   MDVAHCTLDGNADGVEFCPHSSFSNVLAASTYTLQEGEQPSRSGSLSIFNINADIGSLEL 60
           MDVAHCTLDGNADGVEFCPHSSFSNVLAASTYTLQEGEQPSRSGSLSIFN+NADIGSLEL
Sbjct: 1   MDVAHCTLDGNADGVEFCPHSSFSNVLAASTYTLQEGEQPSRSGSLSIFNVNADIGSLEL 60

Query: 61  LHRVETAGIFDIKWSSVGQTASPLLAQADADGCLRIHALECSSNGVEGEYCLKDKCGSKI 120
           LHRVETAGIFDIKWS VGQTASPLLAQADADGCLRIHALECSSNGVEGEY LK+KCGSKI
Sbjct: 61  LHRVETAGIFDIKWSPVGQTASPLLAQADADGCLRIHALECSSNGVEGEY-LKEKCGSKI 120

Query: 121 SSSMCLCLDWNTSASSISVGHSDGSISILSAGESHLEVQNQWKAHEFELWATSFDTHQPH 180
           SSSMCLCLDWNTSASSISVGHSDGSISILSAGESHLEVQNQWKAHEFELWAT+FDTHQPH
Sbjct: 121 SSSMCLCLDWNTSASSISVGHSDGSISILSAGESHLEVQNQWKAHEFELWATAFDTHQPH 180

Query: 181 LVYTGSDDCKFSCWDLRDTPSKVFHNTKVHKMGVCCIAKIPNDPYKLLTGSYDENLRVWD 240
           LVYTGSDDCKFSCWDLRDTPSKVFHNTKVHKMGVCCIAKIPNDPYKLLTGSYDENLRVWD
Sbjct: 181 LVYTGSDDCKFSCWDLRDTPSKVFHNTKVHKMGVCCIAKIPNDPYKLLTGSYDENLRVWD 240

Query: 241 IRAISKPINETSICLGGGVWKIKPHPSVSDVVLAACMHSGFAIVRVKDDGAEVVETYAKH 300
           IRAISKPINETSICLGGGVWKIKPHPSV DVVLAACMH+GFAIVRVK+DGAEVVETYAKH
Sbjct: 241 IRAISKPINETSICLGGGVWKIKPHPSVFDVVLAACMHNGFAIVRVKEDGAEVVETYAKH 300

Query: 301 NSLAYGADWQRGDSSQEDKRKRYAVATCSFYDKLLRIWIPETDIIT 347
           +SLAYGADWQR DSSQEDKRKRYAVATCSFYDKLLRIWIPETDIIT
Sbjct: 301 SSLAYGADWQREDSSQEDKRKRYAVATCSFYDKLLRIWIPETDIIT 345

[Close]

BLAST of CSPI01G01320 vs. NCBI nr
Match: XP_038878593.1 (diphthine methyltransferase homolog [Benincasa hispida])

HSP 1 Score: 677.9 bits (1748), Expect = 4.5e-191
Identity = 319/346 (92.20%), Postives = 331/346 (95.66%), Query Frame = 0


Query: 1   MDVAHCTLDGNADGVEFCPHSSFSNVLAASTYTLQEGEQPSRSGSLSIFNINADIGSLEL 60
           MDVAHCTLDGNAD VEFCPH SFSNVLAASTYTLQEG+QP+RSGSLSIFN+NADIGSLEL
Sbjct: 1   MDVAHCTLDGNADAVEFCPHRSFSNVLAASTYTLQEGDQPTRSGSLSIFNVNADIGSLEL 60

Query: 61  LHRVETAGIFDIKWSSVGQTASPLLAQADADGCLRIHALECSSNGVEGEYCLKDKCGSKI 120
           LHRVETAGIFDIKWS VGQTASPLLAQADADGCLRIHALECSSNGVEGEY LK+KC SKI
Sbjct: 61  LHRVETAGIFDIKWSPVGQTASPLLAQADADGCLRIHALECSSNGVEGEY-LKEKCNSKI 120

Query: 121 SSSMCLCLDWNTSASSISVGHSDGSISILSAGESHLEVQNQWKAHEFELWATSFDTHQPH 180
           SSSMCLCLDWN SA+SISVGHSDGSISILSA ESHLEVQNQWKAHEFELWATSFDTHQPH
Sbjct: 121 SSSMCLCLDWNPSATSISVGHSDGSISILSARESHLEVQNQWKAHEFELWATSFDTHQPH 180

Query: 181 LVYTGSDDCKFSCWDLRDTPSKVFHNTKVHKMGVCCIAKIPNDPYKLLTGSYDENLRVWD 240
           LVYTGSDDCKFSCWDLRDTPSK FHNTK HKMGVCCIAK P+DPYKLLTGSYDENLRVWD
Sbjct: 181 LVYTGSDDCKFSCWDLRDTPSKAFHNTKAHKMGVCCIAKSPSDPYKLLTGSYDENLRVWD 240

Query: 241 IRAISKPINETSICLGGGVWKIKPHPSVSDVVLAACMHSGFAIVRVKDDGAEVVETYAKH 300
           IRAISKP NE S+CLGGGVWKIKPHPSV DV+LAACMH+GFAIVRVKDD AE+VETY KH
Sbjct: 241 IRAISKPTNENSVCLGGGVWKIKPHPSVFDVLLAACMHNGFAIVRVKDDVAEIVETYGKH 300

Query: 301 NSLAYGADWQRGDSSQEDKRKRYAVATCSFYDKLLRIWIPETDIIT 347
           NSLAYGADWQRGDSSQEDKRKRYAVATCSFYD+LLRIWIPETDI+T
Sbjct: 301 NSLAYGADWQRGDSSQEDKRKRYAVATCSFYDRLLRIWIPETDIVT 345

[Close]

BLAST of CSPI01G01320 vs. NCBI nr
Match: XP_023531460.1 (diphthine methyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 652.5 bits (1682), Expect = 2.0e-183
Identity = 309/345 (89.57%), Postives = 326/345 (94.49%), Query Frame = 0


Query: 1   MDVAHCTLDGNADGVEFCPHSSFSNVLAASTYTLQEGEQPSRSGSLSIFNINADIGSLEL 60
           MDVAHCTLDGNAD VEFCPHSSFSNVLAASTYTL EG+QPSRSGSLSIFN+NAD  SLEL
Sbjct: 16  MDVAHCTLDGNADAVEFCPHSSFSNVLAASTYTLLEGDQPSRSGSLSIFNVNADNCSLEL 75

Query: 61  LHRVETAGIFDIKWSSVGQTASPLLAQADADGCLRIHALECSSNGVEGEYCLKDKCGSKI 120
           LHRV+TAGIFDIKWS VG TASPLLAQADADGC+R+HALE SSNG EG+Y LK++CGSKI
Sbjct: 76  LHRVDTAGIFDIKWSPVGPTASPLLAQADADGCIRVHALEHSSNGAEGQY-LKERCGSKI 135

Query: 121 SSSMCLCLDWNTSASSISVGHSDGSISILSAGESHLEVQNQWKAHEFELWATSFDTHQPH 180
           SSSMCLCLDWN SA+SISVGHSDGSISILSAGESHLEVQNQW+AHEFELWATSFDTHQPH
Sbjct: 136 SSSMCLCLDWNPSATSISVGHSDGSISILSAGESHLEVQNQWEAHEFELWATSFDTHQPH 195

Query: 181 LVYTGSDDCKFSCWDLRDTPSKVFHNTKVHKMGVCCIAKIPNDPYKLLTGSYDENLRVWD 240
           LVYTGSDDCKFSCWDLRDTP+K FHNTK HKMGVCCIAK PNDPYKLLTGSYDE+LRVWD
Sbjct: 196 LVYTGSDDCKFSCWDLRDTPTKAFHNTKAHKMGVCCIAKCPNDPYKLLTGSYDEHLRVWD 255

Query: 241 IRAISKPINETSICLGGGVWKIKPHPSVSDVVLAACMHSGFAIVRVKDDG-AEVVETYAK 300
           IR+ISKP NETSICLGGGVW+IKPHPSV DVVLAACMH+GFAIVRVKDD  AEVVETY K
Sbjct: 256 IRSISKPTNETSICLGGGVWRIKPHPSVFDVVLAACMHNGFAIVRVKDDDTAEVVETYDK 315

Query: 301 HNSLAYGADWQRGDSSQEDKRKRYAVATCSFYDKLLRIWIPETDI 345
           H+SLAYGADWQRGDSS EDKRKRYAVATCSFYD+LLRIWIPETDI
Sbjct: 316 HSSLAYGADWQRGDSSHEDKRKRYAVATCSFYDRLLRIWIPETDI 359

[Close]

BLAST of CSPI01G01320 vs. NCBI nr
Match: XP_022928480.1 (diphthine methyltransferase [Cucurbita moschata])

HSP 1 Score: 651.4 bits (1679), Expect = 4.5e-183
Identity = 309/345 (89.57%), Postives = 326/345 (94.49%), Query Frame = 0


Query: 1   MDVAHCTLDGNADGVEFCPHSSFSNVLAASTYTLQEGEQPSRSGSLSIFNINADIGSLEL 60
           MDVAHCTLDGNAD VEFCPHSSFSNVLAASTYTL EG+QPSRSGSLSIFN+NAD  SLEL
Sbjct: 16  MDVAHCTLDGNADAVEFCPHSSFSNVLAASTYTLLEGDQPSRSGSLSIFNVNADNCSLEL 75

Query: 61  LHRVETAGIFDIKWSSVGQTASPLLAQADADGCLRIHALECSSNGVEGEYCLKDKCGSKI 120
           LHRV+TAGIFDIKWS VG TASPLLAQADADGC+R+HALE SSNG EG+Y LK++CGSKI
Sbjct: 76  LHRVDTAGIFDIKWSPVGPTASPLLAQADADGCIRVHALEHSSNGGEGQY-LKERCGSKI 135

Query: 121 SSSMCLCLDWNTSASSISVGHSDGSISILSAGESHLEVQNQWKAHEFELWATSFDTHQPH 180
           SSSMCLCLDWN SA+SISVGHSDGSISILSAGESHLEVQNQW+AHEFELWATSFDTHQPH
Sbjct: 136 SSSMCLCLDWNPSATSISVGHSDGSISILSAGESHLEVQNQWEAHEFELWATSFDTHQPH 195

Query: 181 LVYTGSDDCKFSCWDLRDTPSKVFHNTKVHKMGVCCIAKIPNDPYKLLTGSYDENLRVWD 240
           LVYTGSDDCKFSCWDLRDTP+K FHNTK HKMGVCCIAK PNDPYKLLTGSYDE+LRVWD
Sbjct: 196 LVYTGSDDCKFSCWDLRDTPTKAFHNTKAHKMGVCCIAKCPNDPYKLLTGSYDEHLRVWD 255

Query: 241 IRAISKPINETSICLGGGVWKIKPHPSVSDVVLAACMHSGFAIVRVKDDG-AEVVETYAK 300
           IR+ISKP NETSICLGGGVW+IKPHPSV DVVLAACMH+GFAIVRVKDD  AEVVETY K
Sbjct: 256 IRSISKPTNETSICLGGGVWRIKPHPSVFDVVLAACMHNGFAIVRVKDDDTAEVVETYDK 315

Query: 301 HNSLAYGADWQRGDSSQEDKRKRYAVATCSFYDKLLRIWIPETDI 345
           H+SLAYGADWQRGDSS EDKRKRYAVATCSFYD+LLRIWIPETDI
Sbjct: 316 HSSLAYGADWQRGDSSHEDKRKRYAVATCSFYDRLLRIWIPETDI 359

[Close]

BLAST of CSPI01G01320 vs. TAIR 10
Match: AT5G63010.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 446.4 bits (1147), Expect = 2.0e-125
Identity = 207/350 (59.14%), Postives = 268/350 (76.57%), Query Frame = 0


Query: 1   MDVAHCTLDGNADGVEFCPHSSFSNVLAASTYTLQEGEQPSRSGSLSIFNIN--ADIGSL 60
           MD A C L+GNAD VEFCPH S++N+LAASTYTLQEG+ PSRSGS+ +F++    D+G L
Sbjct: 1   MDAADCYLEGNADAVEFCPHESYANLLAASTYTLQEGDCPSRSGSVYLFDVGDVEDVG-L 60

Query: 61  ELLHRVETAGIFDIKWSSVGQTASPLLAQADADGCLRIHALECSSNGVEGEYCLKDKCGS 120
            L+ +++TAG+FDI+WS  G      LAQADADGCL ++ ++      E +  L++  G 
Sbjct: 61  NLIQKIDTAGVFDIRWSRGGDGGDVALAQADADGCLIVYKID----ETESKGTLREVSGK 120

Query: 121 KISSSMCLCLDWNTSASSISVGHSDGSISILSAGESHLEVQNQWKAHEFELWATSFDTHQ 180
           +ISSSMCLCLDW+ S++SI VG SDGS S++S  +S+LE   +WK H+FELW  SFD + 
Sbjct: 121 RISSSMCLCLDWDPSSTSIVVGLSDGSASVVSFTDSNLETVQEWKGHDFELWTASFDLNN 180

Query: 181 PHLVYTGSDDCKFSCWDLRDTP--SKVFHNTKVHKMGVCCIAKIPNDPYKLLTGSYDENL 240
           P+LVYTGSDDCKFSCWD+RD+P  ++VF N+KVH MGVCCI+  P+DPY + TGSYDE L
Sbjct: 181 PNLVYTGSDDCKFSCWDIRDSPADNRVFQNSKVHTMGVCCISSNPSDPYSIFTGSYDETL 240

Query: 241 RVWDIRAISKPINETSICLGGGVWKIKPHPSVSDVVLAACMHSGFAIVRVKDDGAEVVET 300
           RVWD R++S+P+NETS+ LGGGVW+IK HPS+S VVLAACMH+GFA+ +V D   EV+E+
Sbjct: 241 RVWDTRSVSRPLNETSVSLGGGVWRIKHHPSLSGVVLAACMHNGFALAKVSDGKGEVLES 300

Query: 301 YAKHNSLAYGADWQRGDSSQEDKRKRYAVATCSFYDKLLRIWIPETDIIT 347
           Y KH+SLAYGADW RG       +K+  VATCSFYD+LLR+W+P TD  T
Sbjct: 301 YNKHHSLAYGADWYRGKD-----QKQSVVATCSFYDRLLRVWMPITDFST 340

[Close]

BLAST of CSPI01G01320 vs. TAIR 10
Match: AT2G32950.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 67.0 bits (162), Expect = 3.4e-11
Identity = 43/171 (25.15%), Postives = 79/171 (46.20%), Query Frame = 0


Query: 84  LLAQADADGCLRIHALECSSNGVEGEYC--LKDKCGSKISSSMCLCLDWNT-SASSISVG 143
           L A A    C+++       N      C  ++    SK+S     CL WN    + I+  
Sbjct: 385 LFATAGVSRCIKVFDFSSVVNEPADMQCPIVEMSTRSKLS-----CLSWNKHEKNHIASS 444

Query: 144 HSDGSISI--LSAGESHLEVQNQWKAHEFELWATSFDTHQPHLVYTGSDDCKFSCWDLRD 203
             +G +++  ++  +S +E    ++ HE   W+  F   +P ++ +GSDDCK   W  R 
Sbjct: 445 DYEGIVTVWDVTTRQSLME----YEEHEKRAWSVDFSRTEPSMLVSGSDDCKVKVWCTRQ 504

Query: 204 TPSKVFHNTKVHKMGVCCIAKIPNDPYKLLTGSYDENLRVWDIRAISKPIN 250
             S +  + K +   +CC+   P     +  GS D ++  +D+R IS+P++
Sbjct: 505 EASVINIDMKAN---ICCVKYNPGSSNYIAVGSADHHIHYYDLRNISQPLH 543

[Close]

BLAST of CSPI01G01320 vs. TAIR 10
Match: AT5G58230.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 55.1 bits (131), Expect = 1.3e-07
Identity = 34/122 (27.87%), Postives = 64/122 (52.46%), Query Frame = 0


Query: 156 LEVQNQWKAHEFELWATSFDTHQPHLVYTGSDDCKFSCWDLRD-TPSKVFHNTKVHKMGV 215
           L+ Q  +KAHE  +   ++     +L  +  DD     WDLR  + SK   +   H M V
Sbjct: 217 LDAQQIFKAHEGVVEDVAWHLRHEYLFGSVGDDQYLLIWDLRSPSASKPVQSVVAHSMEV 276

Query: 216 CCIAKIPNDPYKLLTGSYDENLRVWDIRAISKPINETSICLGGGVWKIKPHPSVSDVVLA 275
            C+A  P + + + TGS D+ ++++D+R +S  ++ T       V+++  +P  ++ +LA
Sbjct: 277 NCLAFNPFNEWVVATGSTDKTVKLFDLRKLSTALH-TFDSHKEEVFQVGWNPK-NETILA 336

Query: 276 AC 277
           +C
Sbjct: 337 SC 336

[Close]

BLAST of CSPI01G01320 vs. TAIR 10
Match: AT1G53090.1 (SPA1-related 4 )

HSP 1 Score: 48.1 bits (113), Expect = 1.6e-05
Identity = 36/158 (22.78%), Postives = 65/158 (41.14%), Query Frame = 0


Query: 95  RIHALECSS---NGVEGEYCLKDKCGSKISSSMCLCLDWNT-SASSISVGHSDGSISILS 154
           +I   EC S   +G +  Y + +       S +C    WN+   S ++  + +G + +  
Sbjct: 507 KIKIFECESIIKDGRDIHYPVVELASRSKLSGIC----WNSYIKSQVASSNFEGVVQVWD 566

Query: 155 AGESHLEVQNQWKAHEFELWATSFDTHQPHLVYTGSDDCKFSCWDLRDTPSKVFHNTKVH 214
              + L    + K HE  +W+  + +  P L+ +GSDD     W +    S     TK +
Sbjct: 567 VARNQL--VTEMKEHEKRVWSIDYSSADPTLLASGSDDGSVKLWSINQGVSIGTIKTKAN 626

Query: 215 KMGVCCIAKIPNDPYKLLTGSYDENLRVWDIRAISKPI 249
              +CC+         L  GS D  +  +D+R    P+
Sbjct: 627 ---ICCVQFPSETGRSLAFGSADHKVYYYDLRNPKLPL 655

[Close]

BLAST of CSPI01G01320 vs. TAIR 10
Match: AT1G53090.2 (SPA1-related 4 )

HSP 1 Score: 48.1 bits (113), Expect = 1.6e-05
Identity = 36/158 (22.78%), Postives = 65/158 (41.14%), Query Frame = 0


Query: 95  RIHALECSS---NGVEGEYCLKDKCGSKISSSMCLCLDWNT-SASSISVGHSDGSISILS 154
           +I   EC S   +G +  Y + +       S +C    WN+   S ++  + +G + +  
Sbjct: 507 KIKIFECESIIKDGRDIHYPVVELASRSKLSGIC----WNSYIKSQVASSNFEGVVQVWD 566

Query: 155 AGESHLEVQNQWKAHEFELWATSFDTHQPHLVYTGSDDCKFSCWDLRDTPSKVFHNTKVH 214
              + L    + K HE  +W+  + +  P L+ +GSDD     W +    S     TK +
Sbjct: 567 VARNQL--VTEMKEHEKRVWSIDYSSADPTLLASGSDDGSVKLWSINQGVSIGTIKTKAN 626

Query: 215 KMGVCCIAKIPNDPYKLLTGSYDENLRVWDIRAISKPI 249
              +CC+         L  GS D  +  +D+R    P+
Sbjct: 627 ---ICCVQFPSETGRSLAFGSADHKVYYYDLRNPKLPL 655

The following BLAST results are available for this feature:

BLAST of CSPI01G01320 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q55C80	4.6e-45	31.73	Diphthine methyltransferase homolog OS=Dictyostelium discoideum OX=44689 GN=wdr8...	[more]
Q9BTV6	1.3e-44	33.82	Diphthine methyltransferase OS=Homo sapiens OX=9606 GN=DPH7 PE=1 SV=2	[more]
Q9CYU6	1.9e-43	34.05	Diphthine methyltransferase OS=Mus musculus OX=10090 GN=Dph7 PE=2 SV=1	[more]
P38332	2.4e-25	33.33	Diphthine methyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S2...	[more]
P43254	4.8e-10	25.15	E3 ubiquitin-protein ligase COP1 OS=Arabidopsis thaliana OX=3702 GN=COP1 PE=1 SV...	[more]

BLAST of CSPI01G01320 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LS22	1.2e-205	99.42	WD_REPEATS_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G...	[more]
A0A1S3BVC6	1.3e-199	97.11	diphthine methyltransferase OS=Cucumis melo OX=3656 GN=LOC103493910 PE=4 SV=1	[more]
A0A5A7UUM5	1.3e-199	97.11	Diphthine methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A6J1ERS6	2.2e-183	89.57	diphthine methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111435275 PE=4 S...	[more]
A0A6J1KQV5	1.3e-180	87.79	diphthine methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111497846 PE=4 SV=...	[more]

BLAST of CSPI01G01320 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011656736.1	2.5e-205	99.42	diphthine methyltransferase homolog [Cucumis sativus] >XP_011656781.1 diphthine ...	[more]
XP_008453090.1	2.6e-199	97.11	PREDICTED: diphthine methyltransferase [Cucumis melo] >XP_008453091.1 PREDICTED:...	[more]
XP_038878593.1	4.5e-191	92.20	diphthine methyltransferase homolog [Benincasa hispida]	[more]
XP_023531460.1	2.0e-183	89.57	diphthine methyltransferase [Cucurbita pepo subsp. pepo]	[more]
XP_022928480.1	4.5e-183	89.57	diphthine methyltransferase [Cucurbita moschata]	[more]

BLAST of CSPI01G01320 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G63010.1	2.0e-125	59.14	Transducin/WD40 repeat-like superfamily protein	[more]
AT2G32950.1	3.4e-11	25.15	Transducin/WD40 repeat-like superfamily protein	[more]
AT5G58230.1	1.3e-07	27.87	Transducin/WD40 repeat-like superfamily protein	[more]
AT1G53090.1	1.6e-05	22.78	SPA1-related 4	[more]
AT1G53090.2	1.6e-05	22.78	SPA1-related 4	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001680	WD40 repeat	SMART	SM00320	WD40_4	coord: 155..195 e-value: 0.086 score: 22.0 coord: 113..150 e-value: 41.0 score: 6.8 coord: 57..98 e-value: 62.0 score: 5.7 coord: 290..339 e-value: 130.0 score: 3.7 coord: 200..240 e-value: 2.3E-4 score: 30.5
IPR001680	WD40 repeat	PFAM	PF00400	WD40	coord: 205..240 e-value: 0.0097 score: 16.7
IPR001680	WD40 repeat	PROSITE	PS50082	WD_REPEATS_2	coord: 207..242 score: 9.806668
IPR015943	WD40/YVTN repeat-like-containing domain superfamily	GENE3D	2.130.10.10		coord: 9..338 e-value: 1.2E-28 score: 102.0
None	No IPR available	PANTHER	PTHR46042	DIPHTHINE METHYLTRANSFERASE	coord: 2..343
IPR019775	WD40 repeat, conserved site	PROSITE	PS00678	WD_REPEATS_1	coord: 227..241
IPR036322	WD40-repeat-containing domain superfamily	SUPERFAMILY	50978	WD40 repeat-like	coord: 43..338

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G01320.1	CSPI01G01320.1	mRNA
CSPI01G01320.2	CSPI01G01320.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0005515	protein binding