CSPI01G01180 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G01180
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Kinetochore protein SPC25
Location	Chr1: 735638 .. 738259 (-)
RNA-Seq Expression	CSPI01G01180
Synteny	CSPI01G01180

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGGGCGAACGAGTGTACAGCAACTCCTTTGCTCTGTGAGAGAATGGAGAACAAGGCGAATGATTCTGTTCGTGCGAGGTTGGAAAAACTTCGAATCGTTCGTGAAAATGAAATTCCAGTTCAGCAGCAAAAGATGGAGTCCTTCGCGGATTCATTTTACAAATCAGTGGAATCGATCAGTGTAAGGTCCCGAGCAAATGCCCTAAGTCAAGGTGAGCTATCTATTTTCTTCAATTTACTTGGGAAAGATATGGGTGTGAAACTTGTTACTGTGGTCACTTACGACACTGTAATCGGCATTAGAATGGTTTGTGAAGTGACCATTAGGGTTTTGAAATGGGTAGCGATTAAGACTCAGAATGGATGTAATTCTAGGGAATTGAGTTGGAGTTGGTTCAGAAGTGGCAAAGTTTACTTTGAGATTTCTTGTTTCATTTTTTATTTATTTGGTGATGTATATTTTTTGAATTCTCAGGCAAACTGGGGGAACTAAAAGCGAGGCTGAGGGAGACGGAAGATGAACTGGTTAAAGCTCTTGCAGGTATGAGCTATGATTATGCTACTTTCCTTCAACGCTGTATGAGTTAGAATATACTACTTCGTTGAAGATTGATTCGACTTGAAGTGGCATTTAAGAGTTGAGTTATTTTTCCACTAAAATTGTTGAGAATCTTTTACAACGGTAGTGCTTTGGATTTGGAGCAAGTGATGGGCGAAATGTTGGTGTTTGTGATGCTGGTATATTGTTTCTTACTTAAAATTATCGCTCATCGTAAGGGCTTATTTCCTTTATACTTGGATATTTTTGTAGTGAAGACTCGTAAAGAGGCCAAACGCCTCACAATCATCGACTCCATTGCAACTTCAAAATCCAGAACAGAAGAGCTCAGGAATACTTTACAAGACTGCAAAGCAAGGAGGGATGAATATGCTACAATTATATCTCAGCAATCCTTAGGTAATGTCTGTCAATCTTTTTTTCTTTCAGACCCAGTCTTCCTTTATGCAAACAGAAAACTGCACATGGACTGAAGGTAAATTAAATTGGATATGCATTGATCAATCTTTATTTGCTACTTACTTGCAAGATCTCTTCCACAATTCTTTTTGCAAACTTCAACTTTAGAAGTTCCATACGATAACTGATTACTTCTCTCTCTTTCCAATGCTATCTATAGTTTTATCTTCATCTGAACGCAAGGAAACCCAGGAGAGCGAGTGCAGAAATGAAATACAAGAGGCTATTTCTTGGTACAAAAGGGTTCTTGATTTTCATATTGAAGGAGGATATGGTATGATAATTCAAGTTTGTTTTTGAAGAATCTCAACAGTTTTTGGGAAGCTTGAGATAAATTTGCTGACATCTCTCGGCATTTCAGGGGTGAAATTCTCTTTCAAGAAAATAAATATAGACAATCCTGATGAAGAATATTCTTTTACGATCCGTCATGCAAATGATACTTATATGTGTAAGTGTTATCATACGTGCACTTAATATATGTTGATTTTTCTAGCTCATTATCTTCTGACAACCTGTATTTTTTTTCCTCGACTTTTTAGTGTTAGATTGTAACCCATCCTTGTCAGATATTAAGGAGTTGATACATGAATTAAATAAGACAAATGGTTTGTTCAAGCTTGTCAGGATCATGAGACGGAGATTCCAGGAGGCTGCAGCAGAAGGTGTGCCCTGAGTTTTTAACTTTTCGCTTGAAAGGTATTTTTCCACTAAACGGTTATACGTTTGCTATTCAACAGGAGCTGGGGCTCCAGCATTGTCTCTCCACCAAGGTTCATCAATAATCTCTACGTCAGCCCCAGTCTTGTCATCATCAACCGAGGGAAGTGAATCTTCAACTGAAGAAATTAACATAGATGAAGTTGAGGAAACCAACAAACATTCTAAGAACATTTTACCCAGAAAGAACCCAGCTATCCACTCCAGTGGATCTGCATTTTCTTTGCGTCGATCTCCTCGTTTTAAGGTATGTTCAATTTTAATGTTTGATGTCTGCTGATATTGATTTAGGATTCTCGGGTATATGATACTAAGAGGATACTATAAGTCTATATCTTCTATGTATAACAGGTTGATCTTTTTTTTAAAGCACATTTCAAATTGTTATGCAAAAGAGAATTACTAATAAAATGCACCTCGTATGTGGCAGGTCAGGAAATAAGGTTGGAGGCTGTTAATGATTGAACAAGTATTAGGTTGATGGGCTTAGCCTTTTGCTTCCATGACATGAATTGCTTTGCAATTAACGTTGATTATGCCATTATGGGTCCTGATCTCAACATTTTTCAGAACTTCTCTGACTACTCTGAAGGGTCCATATCATAAAAGTGAAGTTGAAGAAGGCTTTATCCATGACCTGTTGTATGTTCGTTGTATCTGATTTGTTTTTGTGTATTATTTTACACGAAGTTATGGTTTTCATGTAATGCATGGGGTGATTATCCCAAATGTACCGAATGCCAGTACTTTGTATCAATCTGATCTGTTCTAGATTCCACATCATCGGACTTAAATTAAGGTTTGTGCAGTTTTAAATTAATTAATTGTGAAAGCTTGAAGGTGAAACACTGAATGTTCACTTTTCTATGAAATTAAGTTAGTGTA

mRNA sequence

CGGGCGAACGAGTGTACAGCAACTCCTTTGCTCTGTGAGAGAATGGAGAACAAGGCGAATGATTCTGTTCGTGCGAGGTTGGAAAAACTTCGAATCGTTCGTGAAAATGAAATTCCAGTTCAGCAGCAAAAGATGGAGTCCTTCGCGGATTCATTTTACAAATCAGTGGAATCGATCAGTGTAAGGTCCCGAGCAAATGCCCTAAGTCAAGGCAAACTGGGGGAACTAAAAGCGAGGCTGAGGGAGACGGAAGATGAACTGGTTAAAGCTCTTGCAGTGAAGACTCGTAAAGAGGCCAAACGCCTCACAATCATCGACTCCATTGCAACTTCAAAATCCAGAACAGAAGAGCTCAGGAATACTTTACAAGACTGCAAAGCAAGGAGGGATGAATATGCTACAATTATATCTCAGCAATCCTTAGTTTTATCTTCATCTGAACGCAAGGAAACCCAGGAGAGCGAGTGCAGAAATGAAATACAAGAGGCTATTTCTTGGTACAAAAGGGTTCTTGATTTTCATATTGAAGGAGGATATGGGGTGAAATTCTCTTTCAAGAAAATAAATATAGACAATCCTGATGAAGAATATTCTTTTACGATCCGTCATGCAAATGATACTTATATGTTGTTAGATTGTAACCCATCCTTGTCAGATATTAAGGAGTTGATACATGAATTAAATAAGACAAATGGTTTGTTCAAGCTTGTCAGGATCATGAGACGGAGATTCCAGGAGGCTGCAGCAGAAGGAGCTGGGGCTCCAGCATTGTCTCTCCACCAAGGTTCATCAATAATCTCTACGTCAGCCCCAGTCTTGTCATCATCAACCGAGGGAAGTGAATCTTCAACTGAAGAAATTAACATAGATGAAGTTGAGGAAACCAACAAACATTCTAAGAACATTTTACCCAGAAAGAACCCAGCTATCCACTCCAGTGGATCTGCATTTTCTTTGCGTCGATCTCCTCGTTTTAAGGTCAGGAAATAAGGTTGGAGGCTGTTAATGATTGAACAAGTATTAGGTTGATGGGCTTAGCCTTTTGCTTCCATGACATGAATTGCTTTGCAATTAACGTTGATTATGCCATTATGGGTCCTGATCTCAACATTTTTCAGAACTTCTCTGACTACTCTGAAGGGTCCATATCATAAAAGTGAAGTTGAAGAAGGCTTTATCCATGACCTGTTGTATGTTCGTTGTATCTGATTTGTTTTTGTGTATTATTTTACACGAAGTTATGGTTTTCATGTAATGCATGGGGTGATTATCCCAAATGTACCGAATGCCAGTACTTTGTATCAATCTGATCTGTTCTAGATTCCACATCATCGGACTTAAATTAAGGTTTGTGCAGTTTTAAATTAATTAATTGTGAAAGCTTGAAGGTGAAACACTGAATGTTCACTTTTCTATGAAATTAAGTTAGTGTA

Coding sequence (CDS)

ATGGAGAACAAGGCGAATGATTCTGTTCGTGCGAGGTTGGAAAAACTTCGAATCGTTCGTGAAAATGAAATTCCAGTTCAGCAGCAAAAGATGGAGTCCTTCGCGGATTCATTTTACAAATCAGTGGAATCGATCAGTGTAAGGTCCCGAGCAAATGCCCTAAGTCAAGGCAAACTGGGGGAACTAAAAGCGAGGCTGAGGGAGACGGAAGATGAACTGGTTAAAGCTCTTGCAGTGAAGACTCGTAAAGAGGCCAAACGCCTCACAATCATCGACTCCATTGCAACTTCAAAATCCAGAACAGAAGAGCTCAGGAATACTTTACAAGACTGCAAAGCAAGGAGGGATGAATATGCTACAATTATATCTCAGCAATCCTTAGTTTTATCTTCATCTGAACGCAAGGAAACCCAGGAGAGCGAGTGCAGAAATGAAATACAAGAGGCTATTTCTTGGTACAAAAGGGTTCTTGATTTTCATATTGAAGGAGGATATGGGGTGAAATTCTCTTTCAAGAAAATAAATATAGACAATCCTGATGAAGAATATTCTTTTACGATCCGTCATGCAAATGATACTTATATGTTGTTAGATTGTAACCCATCCTTGTCAGATATTAAGGAGTTGATACATGAATTAAATAAGACAAATGGTTTGTTCAAGCTTGTCAGGATCATGAGACGGAGATTCCAGGAGGCTGCAGCAGAAGGAGCTGGGGCTCCAGCATTGTCTCTCCACCAAGGTTCATCAATAATCTCTACGTCAGCCCCAGTCTTGTCATCATCAACCGAGGGAAGTGAATCTTCAACTGAAGAAATTAACATAGATGAAGTTGAGGAAACCAACAAACATTCTAAGAACATTTTACCCAGAAAGAACCCAGCTATCCACTCCAGTGGATCTGCATTTTCTTTGCGTCGATCTCCTCGTTTTAAGGTCAGGAAATAA

Protein sequence

MENKANDSVRARLEKLRIVRENEIPVQQQKMESFADSFYKSVESISVRSRANALSQGKLGELKARLRETEDELVKALAVKTRKEAKRLTIIDSIATSKSRTEELRNTLQDCKARRDEYATIISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRHANDTYMLLDCNPSLSDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGAGAPALSLHQGSSIISTSAPVLSSSTEGSESSTEEINIDEVEETNKHSKNILPRKNPAIHSSGSAFSLRRSPRFKVRK*

Homology

[Close]

BLAST of CSPI01G01180 vs. ExPASy Swiss-Prot
Match: Q93VK9 (Kinetochore protein SPC25 homolog OS=Arabidopsis thaliana OX=3702 GN=SPC25 PE=1 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 4.7e-49
Identity = 131/310 (42.26%), Postives = 190/310 (61.29%), Query Frame = 0


Query: 7   DSVRARLEKLRIVRENEIPVQQQKMESF-ADSFYKSVESISVRSRANALSQGKLGELKAR 66
           D+ +  +  L ++ E +I  Q+ K++SF A  F +S+ S+  R++A A SQ +L  LKA 
Sbjct: 11  DTTKETMASLGLICEKDIHEQRLKIDSFIASPFRRSMNSLVERAQATAQSQVELMNLKAD 70

Query: 67  LRETEDELVKALAVKTRKEAKRLTIIDSIATSKSRTEELRNTLQDCKARRDEYATIISQQ 126
           LRE EDELVK LAVKTRKEA+++ I DSI+ ++SR E LR  LQ  K+++D+   IISQQ
Sbjct: 71  LREAEDELVKVLAVKTRKEARQMGIRDSISATQSRIEVLRRNLQLQKSKKDDSVRIISQQ 130

Query: 127 SLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSF 186
              LS S+    + +E + +I EAISWY   L FH+E G+GVKF+F  I+   P  E+SF
Sbjct: 131 LQALSKSKDNAGKVTEDKADIHEAISWYNHALGFHVEAGHGVKFTFTNIDAKRPTREFSF 190

Query: 187 TIRHANDTYMLLDCNPSLSDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGAGAPALSL 246
           T+ + ND Y LLD +  L  I E++ ELNKTN LF+ VR+MR +F ++        +  L
Sbjct: 191 TVHYGNDIYTLLDSDLQLDYINEMVQELNKTNDLFRFVRLMREQFLKSTLSELPTHSGQL 250

Query: 247 HQGSSIISTSAPVLSSSTEGSESSTEEINIDEVEETNKHSKNILPRKNPAIHSSGSAFSL 306
            Q +S IS SAP +S ST+ + S+ E  N     + N+  K     ++P + +  S  + 
Sbjct: 251 QQETSAISASAPAISFSTDTNMSTPE--NKRSKVQVNRRQKR--GSESPLL-APVSTSAT 310

Query: 307 RRSPRFKVRK 316
           RRS RFK +K
Sbjct: 311 RRSSRFKGKK 315

[Close]

BLAST of CSPI01G01180 vs. ExPASy TrEMBL
Match: A0A0A0LU84 (Kinetochore protein SPC25 OS=Cucumis sativus OX=3659 GN=Csa_1G004230 PE=3 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 6.6e-163
Identity = 313/315 (99.37%), Postives = 315/315 (100.00%), Query Frame = 0


Query: 1   MENKANDSVRARLEKLRIVRENEIPVQQQKMESFADSFYKSVESISVRSRANALSQGKLG 60
           MENKANDSVRARLEKLRI+RENEIPVQQQKMESFADSFYKSVESISVRSRANALSQGKLG
Sbjct: 1   MENKANDSVRARLEKLRILRENEIPVQQQKMESFADSFYKSVESISVRSRANALSQGKLG 60

Query: 61  ELKARLRETEDELVKALAVKTRKEAKRLTIIDSIATSKSRTEELRNTLQDCKARRDEYAT 120
           ELKARLRETEDELVKALAVKTRKEAKRLTIIDSIATSKSRTEELRNTLQDCKARRDEYAT
Sbjct: 61  ELKARLRETEDELVKALAVKTRKEAKRLTIIDSIATSKSRTEELRNTLQDCKARRDEYAT 120

Query: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
           IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD
Sbjct: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180

Query: 181 EEYSFTIRHANDTYMLLDCNPSLSDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGAGA 240
           EEYSFTIRHANDTYMLLDCNPSLSDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGAGA
Sbjct: 181 EEYSFTIRHANDTYMLLDCNPSLSDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGAGA 240

Query: 241 PALSLHQGSSIISTSAPVLSSSTEGSESSTEEINIDEVEETNKHSKNILPRKNPAIHSSG 300
           PALSLHQGSS+ISTSAPVLSSSTEGSESSTEEINIDEVEETNKHSKNILPRKNPAIHSSG
Sbjct: 241 PALSLHQGSSVISTSAPVLSSSTEGSESSTEEINIDEVEETNKHSKNILPRKNPAIHSSG 300

Query: 301 SAFSLRRSPRFKVRK 316
           SAFSLRRSPRFKVRK
Sbjct: 301 SAFSLRRSPRFKVRK 315

[Close]

BLAST of CSPI01G01180 vs. ExPASy TrEMBL
Match: A0A1S3CM71 (Kinetochore protein SPC25 OS=Cucumis melo OX=3656 GN=LOC103502389 PE=3 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 1.9e-154
Identity = 302/315 (95.87%), Postives = 305/315 (96.83%), Query Frame = 0


Query: 1   MENKANDSVRARLEKLRIVRENEIPVQQQKMESFADSFYKSVESISVRSRANALSQGKLG 60
           ME KA DSVRARLEKLRIVRENEIPVQQQKMESFADSF KSVESISVRSRANALSQGKLG
Sbjct: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGKLG 60

Query: 61  ELKARLRETEDELVKALAVKTRKEAKRLTIIDSIATSKSRTEELRNTLQDCKARRDEYAT 120
           ELKARLR  EDELVKALAVKTRKEAKRLTIIDSIATSKSR EELRNTLQDCKARRDEYAT
Sbjct: 61  ELKARLRVAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120

Query: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
           IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD
Sbjct: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180

Query: 181 EEYSFTIRHANDTYMLLDCNPSLSDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGAGA 240
           EEYSFTIRHANDTYMLLDCNPSL DIKELIHELNKTNGLFKLVRIMRRRFQEAAAEG+GA
Sbjct: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGA 240

Query: 241 PALSLHQGSSIISTSAPVLSSSTEGSESSTEEINIDEVEETNKHSKNILPRKNPAIHSSG 300
           PALSLHQGSSIISTSAP LSSSTE S+SSTEEIN+DEVEETNKHSKNILPRK PAIHSSG
Sbjct: 241 PALSLHQGSSIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSG 300

Query: 301 SAFSLRRSPRFKVRK 316
           SAFSLRRSPRFKVRK
Sbjct: 301 SAFSLRRSPRFKVRK 315

[Close]

BLAST of CSPI01G01180 vs. ExPASy TrEMBL
Match: A0A5D3BFE9 (Kinetochore protein SPC25 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001250 PE=3 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 4.3e-146
Identity = 300/364 (82.42%), Postives = 303/364 (83.24%), Query Frame = 0


Query: 1   MENKANDSVRARLEKLRIVRENEIPVQQQKMESFADSFYKSVESISVRSRANALSQG--- 60
           ME KA DSVRARLEKLRIVRENEIPVQQQKMESFADSF KSVESISVRSRANALSQG   
Sbjct: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGELS 60

Query: 61  ------------------------------------------------KLGELKARLRET 120
                                                           KLGELKARLR  
Sbjct: 61  IFFILFGKEMGVKLVTVATYDTVIDIRMVCEVTIRVLKWVAIKTQNGWKLGELKARLRVA 120

Query: 121 EDELVKALAVKTRKEAKRLTIIDSIATSKSRTEELRNTLQDCKARRDEYATIISQQSLVL 180
           EDELVKALAVKTRKEAKRLTIIDSIATSKSR EELRNTLQDCKARRDEYATIISQQSLVL
Sbjct: 121 EDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLVL 180

Query: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240
           SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH
Sbjct: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240

Query: 241 ANDTYMLLDCNPSLSDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGAGAPALSLHQGS 300
           ANDTYMLLDCNPSL DIKELIHELNKTNGLFKLVRIMRRRFQEAAAEG+GAPALSLHQGS
Sbjct: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAPALSLHQGS 300

Query: 301 SIISTSAPVLSSSTEGSESSTEEINIDEVEETNKHSKNILPRKNPAIHSSGSAFSLRRSP 314
           SIISTSAP LSSSTE S+SSTEEIN+DEVEETNKHSKNILPRK PAIHSSGSAFSLRRSP
Sbjct: 301 SIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 360

[Close]

BLAST of CSPI01G01180 vs. ExPASy TrEMBL
Match: A0A5A7UUK0 (Kinetochore protein SPC25 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G001230 PE=3 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 1.3e-145
Identity = 299/364 (82.14%), Postives = 302/364 (82.97%), Query Frame = 0


Query: 1   MENKANDSVRARLEKLRIVRENEIPVQQQKMESFADSFYKSVESISVRSRANALSQG--- 60
           ME KA DSVRARLEKLRIVRENEIPVQQQKMESFADSF KSVESISVRSRANALSQG   
Sbjct: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGELS 60

Query: 61  ------------------------------------------------KLGELKARLRET 120
                                                           KLGELKARLR  
Sbjct: 61  IFFILFGKEMGVKLVTVATYDTVIDIRMVCEVTIRVLKWVAIKTQNGWKLGELKARLRVA 120

Query: 121 EDELVKALAVKTRKEAKRLTIIDSIATSKSRTEELRNTLQDCKARRDEYATIISQQSLVL 180
           EDELVK LAVKTRKEAKRLTIIDSIATSKSR EELRNTLQDCKARRDEYATIISQQSLVL
Sbjct: 121 EDELVKTLAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLVL 180

Query: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240
           SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH
Sbjct: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240

Query: 241 ANDTYMLLDCNPSLSDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGAGAPALSLHQGS 300
           ANDTYMLLDCNPSL DIKELIHELNKTNGLFKLVRIMRRRFQEAAAEG+GAPALSLHQGS
Sbjct: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAPALSLHQGS 300

Query: 301 SIISTSAPVLSSSTEGSESSTEEINIDEVEETNKHSKNILPRKNPAIHSSGSAFSLRRSP 314
           SIISTSAP LSSSTE S+SSTEEIN+DEVEETNKHSKNILPRK PAIHSSGSAFSLRRSP
Sbjct: 301 SIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 360

[Close]

BLAST of CSPI01G01180 vs. ExPASy TrEMBL
Match: A0A6J1BYG5 (Kinetochore protein SPC25 OS=Momordica charantia OX=3673 GN=LOC111006883 PE=3 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 1.8e-128
Identity = 256/316 (81.01%), Postives = 279/316 (88.29%), Query Frame = 0


Query: 1   MENKANDSVRARLEKLRIVRENEIPVQQQKMESFADSFYKSVESISVRSRANALSQGKLG 60
           MENK  DS+R ++E LR+VRE EIPVQQQKMESFADSF KS+ESI+VRSR NALSQGKLG
Sbjct: 1   MENKVEDSIRVKMESLRMVREKEIPVQQQKMESFADSFRKSLESITVRSRENALSQGKLG 60

Query: 61  ELKARLRETEDELVKALAVKTRKEAKRLTIIDSIATSKSRTEELRNTLQDCKARRDEYAT 120
           ELKARLRE EDELVKALAVKTRKEAKRL I DS+A SKSR EELRNTLQDCKA+RDEY+T
Sbjct: 61  ELKARLREAEDELVKALAVKTRKEAKRLAITDSLAASKSRIEELRNTLQDCKAKRDEYST 120

Query: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
           IISQQSL LSSSE KETQES+CR+EIQEAISWY RVLDFHIEGG GVKFSFKKINI+ PD
Sbjct: 121 IISQQSLALSSSEHKETQESDCRSEIQEAISWYNRVLDFHIEGGRGVKFSFKKININIPD 180

Query: 181 EEYSFTIRHANDTYMLLDCNPSLSDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGAGA 240
           +EYSFTIRHA+DTY LLDCNPSL DI++LIHELNKTNGLFK VR+MR+RFQE AAEG GA
Sbjct: 181 KEYSFTIRHASDTYTLLDCNPSLQDIQDLIHELNKTNGLFKFVRVMRQRFQEIAAEGVGA 240

Query: 241 PALSLHQGSSIISTSAPVLSSSTEGSESSTEEI-NIDEVEETNKHSKNILPRKNPAIHSS 300
            ALSLHQ S+IIS SAP LSSST+ SESST+EI N   +EETNKHSK IL  K PA+ S 
Sbjct: 241 QALSLHQDSTIISVSAPGLSSSTDRSESSTQEIDNQVRLEETNKHSKKILSGKKPAVLSP 300

Query: 301 GSAFSLRRSPRFKVRK 316
           GSAFSLRRSPRFKVRK
Sbjct: 301 GSAFSLRRSPRFKVRK 316

[Close]

BLAST of CSPI01G01180 vs. NCBI nr
Match: XP_004138099.1 (kinetochore protein SPC25 homolog [Cucumis sativus] >KGN63556.1 hypothetical protein Csa_014306 [Cucumis sativus])

HSP 1 Score: 583.2 bits (1502), Expect = 1.4e-162
Identity = 313/315 (99.37%), Postives = 315/315 (100.00%), Query Frame = 0


Query: 1   MENKANDSVRARLEKLRIVRENEIPVQQQKMESFADSFYKSVESISVRSRANALSQGKLG 60
           MENKANDSVRARLEKLRI+RENEIPVQQQKMESFADSFYKSVESISVRSRANALSQGKLG
Sbjct: 1   MENKANDSVRARLEKLRILRENEIPVQQQKMESFADSFYKSVESISVRSRANALSQGKLG 60

Query: 61  ELKARLRETEDELVKALAVKTRKEAKRLTIIDSIATSKSRTEELRNTLQDCKARRDEYAT 120
           ELKARLRETEDELVKALAVKTRKEAKRLTIIDSIATSKSRTEELRNTLQDCKARRDEYAT
Sbjct: 61  ELKARLRETEDELVKALAVKTRKEAKRLTIIDSIATSKSRTEELRNTLQDCKARRDEYAT 120

Query: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
           IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD
Sbjct: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180

Query: 181 EEYSFTIRHANDTYMLLDCNPSLSDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGAGA 240
           EEYSFTIRHANDTYMLLDCNPSLSDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGAGA
Sbjct: 181 EEYSFTIRHANDTYMLLDCNPSLSDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGAGA 240

Query: 241 PALSLHQGSSIISTSAPVLSSSTEGSESSTEEINIDEVEETNKHSKNILPRKNPAIHSSG 300
           PALSLHQGSS+ISTSAPVLSSSTEGSESSTEEINIDEVEETNKHSKNILPRKNPAIHSSG
Sbjct: 241 PALSLHQGSSVISTSAPVLSSSTEGSESSTEEINIDEVEETNKHSKNILPRKNPAIHSSG 300

Query: 301 SAFSLRRSPRFKVRK 316
           SAFSLRRSPRFKVRK
Sbjct: 301 SAFSLRRSPRFKVRK 315

[Close]

BLAST of CSPI01G01180 vs. NCBI nr
Match: XP_008464538.1 (PREDICTED: probable kinetochore protein SPC25 [Cucumis melo])

HSP 1 Score: 555.1 bits (1429), Expect = 4.0e-154
Identity = 302/315 (95.87%), Postives = 305/315 (96.83%), Query Frame = 0


Query: 1   MENKANDSVRARLEKLRIVRENEIPVQQQKMESFADSFYKSVESISVRSRANALSQGKLG 60
           ME KA DSVRARLEKLRIVRENEIPVQQQKMESFADSF KSVESISVRSRANALSQGKLG
Sbjct: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGKLG 60

Query: 61  ELKARLRETEDELVKALAVKTRKEAKRLTIIDSIATSKSRTEELRNTLQDCKARRDEYAT 120
           ELKARLR  EDELVKALAVKTRKEAKRLTIIDSIATSKSR EELRNTLQDCKARRDEYAT
Sbjct: 61  ELKARLRVAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120

Query: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
           IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD
Sbjct: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180

Query: 181 EEYSFTIRHANDTYMLLDCNPSLSDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGAGA 240
           EEYSFTIRHANDTYMLLDCNPSL DIKELIHELNKTNGLFKLVRIMRRRFQEAAAEG+GA
Sbjct: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGA 240

Query: 241 PALSLHQGSSIISTSAPVLSSSTEGSESSTEEINIDEVEETNKHSKNILPRKNPAIHSSG 300
           PALSLHQGSSIISTSAP LSSSTE S+SSTEEIN+DEVEETNKHSKNILPRK PAIHSSG
Sbjct: 241 PALSLHQGSSIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSG 300

Query: 301 SAFSLRRSPRFKVRK 316
           SAFSLRRSPRFKVRK
Sbjct: 301 SAFSLRRSPRFKVRK 315

[Close]

BLAST of CSPI01G01180 vs. NCBI nr
Match: TYJ98520.1 (putative kinetochore protein SPC25 [Cucumis melo var. makuwa])

HSP 1 Score: 527.3 bits (1357), Expect = 8.9e-146
Identity = 300/364 (82.42%), Postives = 303/364 (83.24%), Query Frame = 0


Query: 1   MENKANDSVRARLEKLRIVRENEIPVQQQKMESFADSFYKSVESISVRSRANALSQG--- 60
           ME KA DSVRARLEKLRIVRENEIPVQQQKMESFADSF KSVESISVRSRANALSQG   
Sbjct: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGELS 60

Query: 61  ------------------------------------------------KLGELKARLRET 120
                                                           KLGELKARLR  
Sbjct: 61  IFFILFGKEMGVKLVTVATYDTVIDIRMVCEVTIRVLKWVAIKTQNGWKLGELKARLRVA 120

Query: 121 EDELVKALAVKTRKEAKRLTIIDSIATSKSRTEELRNTLQDCKARRDEYATIISQQSLVL 180
           EDELVKALAVKTRKEAKRLTIIDSIATSKSR EELRNTLQDCKARRDEYATIISQQSLVL
Sbjct: 121 EDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLVL 180

Query: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240
           SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH
Sbjct: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240

Query: 241 ANDTYMLLDCNPSLSDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGAGAPALSLHQGS 300
           ANDTYMLLDCNPSL DIKELIHELNKTNGLFKLVRIMRRRFQEAAAEG+GAPALSLHQGS
Sbjct: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAPALSLHQGS 300

Query: 301 SIISTSAPVLSSSTEGSESSTEEINIDEVEETNKHSKNILPRKNPAIHSSGSAFSLRRSP 314
           SIISTSAP LSSSTE S+SSTEEIN+DEVEETNKHSKNILPRK PAIHSSGSAFSLRRSP
Sbjct: 301 SIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 360

[Close]

BLAST of CSPI01G01180 vs. NCBI nr
Match: KAA0057836.1 (putative kinetochore protein SPC25 [Cucumis melo var. makuwa])

HSP 1 Score: 525.8 bits (1353), Expect = 2.6e-145
Identity = 299/364 (82.14%), Postives = 302/364 (82.97%), Query Frame = 0


Query: 1   MENKANDSVRARLEKLRIVRENEIPVQQQKMESFADSFYKSVESISVRSRANALSQG--- 60
           ME KA DSVRARLEKLRIVRENEIPVQQQKMESFADSF KSVESISVRSRANALSQG   
Sbjct: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGELS 60

Query: 61  ------------------------------------------------KLGELKARLRET 120
                                                           KLGELKARLR  
Sbjct: 61  IFFILFGKEMGVKLVTVATYDTVIDIRMVCEVTIRVLKWVAIKTQNGWKLGELKARLRVA 120

Query: 121 EDELVKALAVKTRKEAKRLTIIDSIATSKSRTEELRNTLQDCKARRDEYATIISQQSLVL 180
           EDELVK LAVKTRKEAKRLTIIDSIATSKSR EELRNTLQDCKARRDEYATIISQQSLVL
Sbjct: 121 EDELVKTLAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLVL 180

Query: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240
           SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH
Sbjct: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240

Query: 241 ANDTYMLLDCNPSLSDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGAGAPALSLHQGS 300
           ANDTYMLLDCNPSL DIKELIHELNKTNGLFKLVRIMRRRFQEAAAEG+GAPALSLHQGS
Sbjct: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAPALSLHQGS 300

Query: 301 SIISTSAPVLSSSTEGSESSTEEINIDEVEETNKHSKNILPRKNPAIHSSGSAFSLRRSP 314
           SIISTSAP LSSSTE S+SSTEEIN+DEVEETNKHSKNILPRK PAIHSSGSAFSLRRSP
Sbjct: 301 SIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 360

[Close]

BLAST of CSPI01G01180 vs. NCBI nr
Match: XP_038880541.1 (kinetochore protein SPC25 homolog [Benincasa hispida])

HSP 1 Score: 505.0 bits (1299), Expect = 4.7e-139
Identity = 279/316 (88.29%), Postives = 290/316 (91.77%), Query Frame = 0


Query: 1   MENKANDSVRARLEKLRIVRENEIPVQQQKMESFADSFYKSVESISVRSRANALSQGKLG 60
           MENKA DSVRARLEKLRIVRE EIP QQQKMESF +SFYKSVESI+VRSRANALSQGKLG
Sbjct: 1   MENKAKDSVRARLEKLRIVREKEIPFQQQKMESFVNSFYKSVESINVRSRANALSQGKLG 60

Query: 61  ELKARLRETEDELVKALAVKTRKEAKRLTIIDSIATSKSRTEELRNTLQDCKARRDEYAT 120
           ELKARLRE EDELVKALAVKTRKEAKRLTIIDS+ATSKSR EELRNTLQD KARRDEY+T
Sbjct: 61  ELKARLREAEDELVKALAVKTRKEAKRLTIIDSLATSKSRIEELRNTLQDSKARRDEYST 120

Query: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
           IIS+QSL LSSSERKE QESECR EIQEAISWYKRVLDFHIEGG+GVKFSFKKIN+ NPD
Sbjct: 121 IISRQSLALSSSERKEPQESECRKEIQEAISWYKRVLDFHIEGGHGVKFSFKKINVVNPD 180

Query: 181 EEYSFTIRHANDTYMLLDCNPSLSDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGAGA 240
           EEYSFTIRHANDTYMLLDCNPSL DIKELIHELNKTNGLFKLVRIMRRRFQEAAA+G  A
Sbjct: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAKGVEA 240

Query: 241 PALSLHQGSSIISTSAPVLSSSTEGSESSTEEIN-IDEVEETNKHSKNILPRKNPAIHSS 300
            ALSLHQGS II  SAP LSSSTE SESS+EEIN  DE+EETNKH+K ILP K PAI SS
Sbjct: 241 QALSLHQGSMIIPMSAPGLSSSTERSESSSEEINKKDELEETNKHTKKILPGKKPAIKSS 300

Query: 301 GSAFSLRRSPRFKVRK 316
           GSAFSLRRSPRFKVRK
Sbjct: 301 GSAFSLRRSPRFKVRK 316

[Close]

BLAST of CSPI01G01180 vs. TAIR 10
Match: AT3G48210.1 (CONTAINS InterPro DOMAIN/s: Kinetochore-Ndc80 complex, subunit Spc25 (InterPro:IPR013255); Has 194 Blast hits to 194 proteins in 72 species: Archae - 0; Bacteria - 4; Metazoa - 72; Fungi - 39; Plants - 62; Viruses - 0; Other Eukaryotes - 17 (source: NCBI BLink). )

HSP 1 Score: 196.4 bits (498), Expect = 3.4e-50
Identity = 131/310 (42.26%), Postives = 190/310 (61.29%), Query Frame = 0


Query: 7   DSVRARLEKLRIVRENEIPVQQQKMESF-ADSFYKSVESISVRSRANALSQGKLGELKAR 66
           D+ +  +  L ++ E +I  Q+ K++SF A  F +S+ S+  R++A A SQ +L  LKA 
Sbjct: 11  DTTKETMASLGLICEKDIHEQRLKIDSFIASPFRRSMNSLVERAQATAQSQVELMNLKAD 70

Query: 67  LRETEDELVKALAVKTRKEAKRLTIIDSIATSKSRTEELRNTLQDCKARRDEYATIISQQ 126
           LRE EDELVK LAVKTRKEA+++ I DSI+ ++SR E LR  LQ  K+++D+   IISQQ
Sbjct: 71  LREAEDELVKVLAVKTRKEARQMGIRDSISATQSRIEVLRRNLQLQKSKKDDSVRIISQQ 130

Query: 127 SLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSF 186
              LS S+    + +E + +I EAISWY   L FH+E G+GVKF+F  I+   P  E+SF
Sbjct: 131 LQALSKSKDNAGKVTEDKADIHEAISWYNHALGFHVEAGHGVKFTFTNIDAKRPTREFSF 190

Query: 187 TIRHANDTYMLLDCNPSLSDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGAGAPALSL 246
           T+ + ND Y LLD +  L  I E++ ELNKTN LF+ VR+MR +F ++        +  L
Sbjct: 191 TVHYGNDIYTLLDSDLQLDYINEMVQELNKTNDLFRFVRLMREQFLKSTLSELPTHSGQL 250

Query: 247 HQGSSIISTSAPVLSSSTEGSESSTEEINIDEVEETNKHSKNILPRKNPAIHSSGSAFSL 306
            Q +S IS SAP +S ST+ + S+ E  N     + N+  K     ++P + +  S  + 
Sbjct: 251 QQETSAISASAPAISFSTDTNMSTPE--NKRSKVQVNRRQKR--GSESPLL-APVSTSAT 310

Query: 307 RRSPRFKVRK 316
           RRS RFK +K
Sbjct: 311 RRSSRFKGKK 315

The following BLAST results are available for this feature:

BLAST of CSPI01G01180 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93VK9	4.7e-49	42.26	Kinetochore protein SPC25 homolog OS=Arabidopsis thaliana OX=3702 GN=SPC25 PE=1 ...	[more]

BLAST of CSPI01G01180 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LU84	6.6e-163	99.37	Kinetochore protein SPC25 OS=Cucumis sativus OX=3659 GN=Csa_1G004230 PE=3 SV=1	[more]
A0A1S3CM71	1.9e-154	95.87	Kinetochore protein SPC25 OS=Cucumis melo OX=3656 GN=LOC103502389 PE=3 SV=1	[more]
A0A5D3BFE9	4.3e-146	82.42	Kinetochore protein SPC25 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A5A7UUK0	1.3e-145	82.14	Kinetochore protein SPC25 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo...	[more]
A0A6J1BYG5	1.8e-128	81.01	Kinetochore protein SPC25 OS=Momordica charantia OX=3673 GN=LOC111006883 PE=3 SV...	[more]

BLAST of CSPI01G01180 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004138099.1	1.4e-162	99.37	kinetochore protein SPC25 homolog [Cucumis sativus] >KGN63556.1 hypothetical pro...	[more]
XP_008464538.1	4.0e-154	95.87	PREDICTED: probable kinetochore protein SPC25 [Cucumis melo]	[more]
TYJ98520.1	8.9e-146	82.42	putative kinetochore protein SPC25 [Cucumis melo var. makuwa]	[more]
KAA0057836.1	2.6e-145	82.14	putative kinetochore protein SPC25 [Cucumis melo var. makuwa]	[more]
XP_038880541.1	4.7e-139	88.29	kinetochore protein SPC25 homolog [Benincasa hispida]	[more]

BLAST of CSPI01G01180 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G48210.1	3.4e-50	42.26	CONTAINS InterPro DOMAIN/s: Kinetochore-Ndc80 complex, subunit Spc25 (InterPro:I...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 52..86
None	No IPR available	GENE3D	3.30.457.50	Chromosome segregation protein Spc25	coord: 138..237 e-value: 1.3E-24 score: 88.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 256..315
None	No IPR available	PANTHER	PTHR14281:SF3	SUBFAMILY NOT NAMED	coord: 1..313
IPR013255	Chromosome segregation protein Spc25, C-terminal	PFAM	PF08234	Spindle_Spc25	coord: 166..231 e-value: 3.5E-20 score: 72.0
IPR045143	Kinetochore protein Spc25	PANTHER	PTHR14281	KINETOCHORE PROTEIN SPC25-RELATED	coord: 1..313

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G01180.1	CSPI01G01180.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007049	cell cycle
biological_process	GO:0051301	cell division
biological_process	GO:0007059	chromosome segregation
cellular_component	GO:0031262	Ndc80 complex
cellular_component	GO:0005634	nucleus