BhiUN533G10 (gene) Wax gourd (B227) v1

Overview


Name	BhiUN533G10
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	enoyl-CoA delta isomerase 2, peroxisomal-like
Location	Contig533: 248104 .. 248322 (+)
RNA-Seq Expression	BhiUN533G10
Synteny	BhiUN533G10

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGTGAAAGGCGAAGCGGCGGTGAGGATGGGGATCGTGGAATCGGCTCACGACGGCGAGGACGGCGTCATGGAGGCTGCCGTACGACTCGGGGAGCAGTTGGGGGCGAGAAATTGGAACGGTGAACCCTACGCGGAAATTAGGAAAAGTCTTTACCCAGAGATCTCTCGGCTGCTTGGATTGCCGGAGAAGGCCATATCGATTTCCAAGCTATGA

mRNA sequence

ATGAAAGTGAAAGGCGAAGCGGCGGTGAGGATGGGGATCGTGGAATCGGCTCACGACGGCGAGGACGGCGTCATGGAGGCTGCCGTACGACTCGGGGAGCAGTTGGGGGCGAGAAATTGGAACGGTGAACCCTACGCGGAAATTAGGAAAAGTCTTTACCCAGAGATCTCTCGGCTGCTTGGATTGCCGGAGAAGGCCATATCGATTTCCAAGCTATGA

Coding sequence (CDS)

ATGAAAGTGAAAGGCGAAGCGGCGGTGAGGATGGGGATCGTGGAATCGGCTCACGACGGCGAGGACGGCGTCATGGAGGCTGCCGTACGACTCGGGGAGCAGTTGGGGGCGAGAAATTGGAACGGTGAACCCTACGCGGAAATTAGGAAAAGTCTTTACCCAGAGATCTCTCGGCTGCTTGGATTGCCGGAGAAGGCCATATCGATTTCCAAGCTATGA

Protein sequence

MKVKGEAAVRMGIVESAHDGEDGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLLGLPEKAISISKL

Homology

[Close]

BLAST of BhiUN533G10 vs. TAIR 10
Match: AT4G14430.1 (indole-3-butyric acid response 10 )

HSP 1 Score: 80.9 bits (198), Expect = 4.7e-16
Identity = 40/72 (55.56%), Postives = 55/72 (76.39%), Query Frame = 0


Query: 2   KVKGEAAVRMGIVES-AHDGEDGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLL 61
           K++GE AV +GIV+S A+D E+GV+ A+VRLGE+L A+ W+GE YA IRKSLYPE+  +L
Sbjct: 169 KIRGEEAVGLGIVDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGIL 228

Query: 62  GLPEKAISISKL 73
           GL  +  +  KL
Sbjct: 229 GLETRVFATPKL 240

[Close]

BLAST of BhiUN533G10 vs. TAIR 10
Match: AT4G14440.1 (3-hydroxyacyl-CoA dehydratase 1 )

HSP 1 Score: 79.0 bits (193), Expect = 1.8e-15
Identity = 40/72 (55.56%), Postives = 51/72 (70.83%), Query Frame = 0


Query: 2   KVKGEAAVRMGIVES-AHDGEDGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLL 61
           K+KGE AV +GIV+S AHD  +GV+EA V LGE L A+ WNGE YA IRKSLYPE+ R++
Sbjct: 167 KLKGEEAVALGIVDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMV 226

Query: 62  GLPEKAISISKL 73
            L    ++   L
Sbjct: 227 DLTANNLATHNL 238

[Close]

BLAST of BhiUN533G10 vs. TAIR 10
Match: AT1G65520.1 (delta(3), delta(2)-enoyl CoA isomerase 1 )

HSP 1 Score: 46.6 bits (109), Expect = 9.8e-06
Identity = 22/55 (40.00%), Postives = 36/55 (65.45%), Query Frame = 0


Query: 2   KVKGEAAVRMGIVESAHDGEDGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEI 57
           KV  +  V+MGIV+SA+      +EAA++LGE++  R  +G  Y ++R+SL  E+
Sbjct: 164 KVTADVGVKMGIVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

[Close]

BLAST of BhiUN533G10 vs. ExPASy Swiss-Prot
Match: O23299 (Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2 PE=1 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 6.6e-15
Identity = 40/72 (55.56%), Postives = 55/72 (76.39%), Query Frame = 0


Query: 2   KVKGEAAVRMGIVES-AHDGEDGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLL 61
           K++GE AV +GIV+S A+D E+GV+ A+VRLGE+L A+ W+GE YA IRKSLYPE+  +L
Sbjct: 169 KIRGEEAVGLGIVDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGIL 228

Query: 62  GLPEKAISISKL 73
           GL  +  +  KL
Sbjct: 229 GLETRVFATPKL 240

[Close]

BLAST of BhiUN533G10 vs. ExPASy Swiss-Prot
Match: O23300 (Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 2.5e-14
Identity = 40/72 (55.56%), Postives = 51/72 (70.83%), Query Frame = 0


Query: 2   KVKGEAAVRMGIVES-AHDGEDGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLL 61
           K+KGE AV +GIV+S AHD  +GV+EA V LGE L A+ WNGE YA IRKSLYPE+ R++
Sbjct: 167 KLKGEEAVALGIVDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMV 226

Query: 62  GLPEKAISISKL 73
            L    ++   L
Sbjct: 227 DLTANNLATHNL 238

[Close]

BLAST of BhiUN533G10 vs. ExPASy Swiss-Prot
Match: O04469 (Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1 PE=1 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 1.4e-04
Identity = 22/55 (40.00%), Postives = 36/55 (65.45%), Query Frame = 0


Query: 2   KVKGEAAVRMGIVESAHDGEDGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEI 57
           KV  +  V+MGIV+SA+      +EAA++LGE++  R  +G  Y ++R+SL  E+
Sbjct: 164 KVTADVGVKMGIVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

[Close]

BLAST of BhiUN533G10 vs. NCBI nr
Match: XP_038877347.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Benincasa hispida])

HSP 1 Score: 138.3 bits (347), Expect = 2.7e-29
Identity = 69/72 (95.83%), Postives = 69/72 (95.83%), Query Frame = 0


Query: 1   MKVKGEAAVRMGIVESAHDGEDGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLL 60
           MKVKGEAAVRMGIVES HDGEDGVMEAAVR GEQLGARNWNGEPYAEIRKSLYPEISRLL
Sbjct: 168 MKVKGEAAVRMGIVESVHDGEDGVMEAAVRFGEQLGARNWNGEPYAEIRKSLYPEISRLL 227

Query: 61  GLPEKAISISKL 73
           GLPEK ISISKL
Sbjct: 228 GLPEKVISISKL 239

[Close]

BLAST of BhiUN533G10 vs. NCBI nr
Match: XP_004134080.1 (enoyl-CoA delta isomerase 2, peroxisomal [Cucumis sativus] >KGN56882.1 hypothetical protein Csa_009715 [Cucumis sativus])

HSP 1 Score: 132.9 bits (333), Expect = 1.1e-27
Identity = 66/72 (91.67%), Postives = 69/72 (95.83%), Query Frame = 0


Query: 1   MKVKGEAAVRMGIVESAHDGEDGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLL 60
           MKVKGEAAV+MGI +SAHDGE+GVMEAAVRLGEQLG R WNGEPYAEIRKSLYPEISRLL
Sbjct: 168 MKVKGEAAVKMGIADSAHDGENGVMEAAVRLGEQLGERKWNGEPYAEIRKSLYPEISRLL 227

Query: 61  GLPEKAISISKL 73
           GLPEKAISISKL
Sbjct: 228 GLPEKAISISKL 239

[Close]

BLAST of BhiUN533G10 vs. NCBI nr
Match: XP_008438558.1 (PREDICTED: enoyl-CoA delta isomerase 2, peroxisomal-like [Cucumis melo])

HSP 1 Score: 132.1 bits (331), Expect = 1.9e-27
Identity = 66/72 (91.67%), Postives = 69/72 (95.83%), Query Frame = 0


Query: 1   MKVKGEAAVRMGIVESAHDGEDGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLL 60
           MKVKGEAAV+MGI +SA+DGE GVMEAAVRLGEQLGAR WNGEPYAEIRKSLYPEISRLL
Sbjct: 168 MKVKGEAAVKMGIADSAYDGESGVMEAAVRLGEQLGARKWNGEPYAEIRKSLYPEISRLL 227

Query: 61  GLPEKAISISKL 73
           GLPEKAISISKL
Sbjct: 228 GLPEKAISISKL 239

[Close]

BLAST of BhiUN533G10 vs. NCBI nr
Match: KAA0049275.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucumis melo var. makuwa] >TYK17282.1 enoyl-CoA delta isomerase 2, peroxisomal-like [Cucumis melo var. makuwa])

HSP 1 Score: 130.6 bits (327), Expect = 5.5e-27
Identity = 65/72 (90.28%), Postives = 68/72 (94.44%), Query Frame = 0


Query: 1   MKVKGEAAVRMGIVESAHDGEDGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLL 60
           MKVKGEAAV+MGI +S +DGE GVMEAAVRLGEQLGAR WNGEPYAEIRKSLYPEISRLL
Sbjct: 168 MKVKGEAAVKMGIADSVYDGESGVMEAAVRLGEQLGARKWNGEPYAEIRKSLYPEISRLL 227

Query: 61  GLPEKAISISKL 73
           GLPEKAISISKL
Sbjct: 228 GLPEKAISISKL 239

[Close]

BLAST of BhiUN533G10 vs. NCBI nr
Match: XP_022980470.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita maxima])

HSP 1 Score: 128.3 bits (321), Expect = 2.8e-26
Identity = 64/72 (88.89%), Postives = 67/72 (93.06%), Query Frame = 0


Query: 1   MKVKGEAAVRMGIVESAHDGEDGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLL 60
           MKVKGE+AVRMGIVESAH+G DGVM+AAVRLGEQL  RNWNGEPYAEIRKSLYPEISRLL
Sbjct: 168 MKVKGESAVRMGIVESAHEGADGVMKAAVRLGEQLAERNWNGEPYAEIRKSLYPEISRLL 227

Query: 61  GLPEKAISISKL 73
           GLPEK  SISKL
Sbjct: 228 GLPEKFFSISKL 239

[Close]

BLAST of BhiUN533G10 vs. ExPASy TrEMBL
Match: A0A0A0L8C7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141780 PE=4 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 5.4e-28
Identity = 66/72 (91.67%), Postives = 69/72 (95.83%), Query Frame = 0


Query: 1   MKVKGEAAVRMGIVESAHDGEDGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLL 60
           MKVKGEAAV+MGI +SAHDGE+GVMEAAVRLGEQLG R WNGEPYAEIRKSLYPEISRLL
Sbjct: 168 MKVKGEAAVKMGIADSAHDGENGVMEAAVRLGEQLGERKWNGEPYAEIRKSLYPEISRLL 227

Query: 61  GLPEKAISISKL 73
           GLPEKAISISKL
Sbjct: 228 GLPEKAISISKL 239

[Close]

BLAST of BhiUN533G10 vs. ExPASy TrEMBL
Match: A0A1S3AWS8 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC103483623 PE=4 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 9.2e-28
Identity = 66/72 (91.67%), Postives = 69/72 (95.83%), Query Frame = 0


Query: 1   MKVKGEAAVRMGIVESAHDGEDGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLL 60
           MKVKGEAAV+MGI +SA+DGE GVMEAAVRLGEQLGAR WNGEPYAEIRKSLYPEISRLL
Sbjct: 168 MKVKGEAAVKMGIADSAYDGESGVMEAAVRLGEQLGARKWNGEPYAEIRKSLYPEISRLL 227

Query: 61  GLPEKAISISKL 73
           GLPEKAISISKL
Sbjct: 228 GLPEKAISISKL 239

[Close]

BLAST of BhiUN533G10 vs. ExPASy TrEMBL
Match: A0A5A7U6I8 (Enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001360 PE=4 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 2.7e-27
Identity = 65/72 (90.28%), Postives = 68/72 (94.44%), Query Frame = 0


Query: 1   MKVKGEAAVRMGIVESAHDGEDGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLL 60
           MKVKGEAAV+MGI +S +DGE GVMEAAVRLGEQLGAR WNGEPYAEIRKSLYPEISRLL
Sbjct: 168 MKVKGEAAVKMGIADSVYDGESGVMEAAVRLGEQLGARKWNGEPYAEIRKSLYPEISRLL 227

Query: 61  GLPEKAISISKL 73
           GLPEKAISISKL
Sbjct: 228 GLPEKAISISKL 239

[Close]

BLAST of BhiUN533G10 vs. ExPASy TrEMBL
Match: A0A6J1IWE7 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC111479854 PE=4 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.3e-26
Identity = 64/72 (88.89%), Postives = 67/72 (93.06%), Query Frame = 0


Query: 1   MKVKGEAAVRMGIVESAHDGEDGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLL 60
           MKVKGE+AVRMGIVESAH+G DGVM+AAVRLGEQL  RNWNGEPYAEIRKSLYPEISRLL
Sbjct: 168 MKVKGESAVRMGIVESAHEGADGVMKAAVRLGEQLAERNWNGEPYAEIRKSLYPEISRLL 227

Query: 61  GLPEKAISISKL 73
           GLPEK  SISKL
Sbjct: 228 GLPEKFFSISKL 239

[Close]

BLAST of BhiUN533G10 vs. ExPASy TrEMBL
Match: A0A6J1EBT7 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=LOC111431760 PE=4 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 2.5e-25
Identity = 62/72 (86.11%), Postives = 65/72 (90.28%), Query Frame = 0


Query: 1   MKVKGEAAVRMGIVESAHDGEDGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLL 60
           MKV GE AVRMGIVESAH+G DGVM+AAVRLGEQL  RNWNGEPYAEIRKSL+PEISRLL
Sbjct: 168 MKVTGEPAVRMGIVESAHEGADGVMKAAVRLGEQLAERNWNGEPYAEIRKSLHPEISRLL 227

Query: 61  GLPEKAISISKL 73
           GLPEK  SISKL
Sbjct: 228 GLPEKVFSISKL 239

The following BLAST results are available for this feature:

BLAST of BhiUN533G10 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G14430.1	4.7e-16	55.56	indole-3-butyric acid response 10	[more]
AT4G14440.1	1.8e-15	55.56	3-hydroxyacyl-CoA dehydratase 1	[more]
AT1G65520.1	9.8e-06	40.00	delta(3), delta(2)-enoyl CoA isomerase 1	[more]

BLAST of BhiUN533G10 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23299	6.6e-15	55.56	Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2...	[more]
O23300	2.5e-14	55.56	Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1	[more]
O04469	1.4e-04	40.00	Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1...	[more]

BLAST of BhiUN533G10 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038877347.1	2.7e-29	95.83	enoyl-CoA delta isomerase 2, peroxisomal-like [Benincasa hispida]	[more]
XP_004134080.1	1.1e-27	91.67	enoyl-CoA delta isomerase 2, peroxisomal [Cucumis sativus] >KGN56882.1 hypotheti...	[more]
XP_008438558.1	1.9e-27	91.67	PREDICTED: enoyl-CoA delta isomerase 2, peroxisomal-like [Cucumis melo]	[more]
KAA0049275.1	5.5e-27	90.28	enoyl-CoA delta isomerase 2, peroxisomal-like [Cucumis melo var. makuwa] >TYK172...	[more]
XP_022980470.1	2.8e-26	88.89	enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita maxima]	[more]

BLAST of BhiUN533G10 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L8C7	5.4e-28	91.67	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141780 PE=4 SV=1	[more]
A0A1S3AWS8	9.2e-28	91.67	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A5A7U6I8	2.7e-27	90.28	Enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo var. makuwa OX=119...	[more]
A0A6J1IWE7	1.3e-26	88.89	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1EBT7	2.5e-25	86.11	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=L...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR11941	ENOYL-COA HYDRATASE-RELATED	coord: 1..66
None	No IPR available	PANTHER	PTHR11941:SF75	ENOYL-COA DELTA ISOMERASE 2, PEROXISOMAL	coord: 1..66

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
BhiUN533M10	BhiUN533M10	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006635	fatty acid beta-oxidation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004165	dodecenoyl-CoA delta-isomerase activity
molecular_function	GO:0004300	enoyl-CoA hydratase activity