BhiUN32G25 (gene) Wax gourd (B227) v1

Overview
NameBhiUN32G25
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionLipoxygenase
LocationContig32: 550368 .. 552580 (+)
RNA-Seq ExpressionBhiUN32G25
SyntenyBhiUN32G25
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGGAAAACAGGCTGTATATATTGGATCACCATGATTCAATAATTCCATTTCTAAGAAGAATAAATTCATTAAATTCCACAAAAACATATGCCACAAGAACAATTCTGTTCCTAAAAGATGATGGGACTTTGAAGCCTTTGGCAATTGAATTAAGCTTACCCCACCCAGATGGAGACAAATTTGGTGCCATTAGCAGAGTAGTTTTGGCAGCTAAAGAAGGAGTTGATGCCTCAATTTGGCAATTGGCAAAGGCTTATGTTGCTGTTAATGATACTGGCCACCATCAACTTGTTAGTCACTGGTAACCTCTTAAAACAAACTCTCAACTCTTAAAAAACCCTAAGGATTATATATTTATAATTGTATTACATTTTCAAAATTTAAAGTAGTTTTCCAATTTTATCCTTCTTTTCACATATTAAGTAAGGGTATTTTGTAAATAACCTGAATCTTGAACCAAAGAACCTGCGTTCTTTGTCAGCAGAGCTTTGATTTTAAGGCAACCCATATCTAGTCCAATCGTCGGAGTTGAAAATCAAATTGATTTGAGGATTTTAGTATTGAGAATCAAGGTCCACATATTCAAGGTGTTCTTTGTTCAACCTTAATTTGGTTTTTATATTAATCAACTCATTTTAATTTGTATTTATGTGTGTATAGGTTGAACACCCATGCAGTAATTGAGCCATTTGTGATTGCAACCAACAGACAATTGAGTGTTCTTCATCCCATTCACAAGTTGCTAGTGCCTCATTTTAAGGATACCATGAAAATCAATGCTCTTGCAAGGGAATCTCTTATTAGTGCTAATGGCATCATTGAGAGAACTCATTATCCAGCTAAATATTCCATGGAGATGTCTTCTTTTGCTTACAAAACTTGGGTCTTCCCTCAACAAGCTCTTCCTGCTGATTTGATTGAGAGGTAAACCATTTTCATCTCTTCAATTTTGTTTTGAATTCTACTACAAGAAAAAAGATTTCTCGACGCCAAAACATGCATCACGAGAAAAGCTATGGGGAGAAAGTGTGTCTCCCGACGCATCTTGGCTGCCCCCGGTAAATGTGAAGAACTCCCAACATTGTATAATTGTCCTTATGAGATATAGTTGAAGTTTGGATATATATGAGGTTCAAATACAAAAGTACTCTTGAATTTCGCCGCTGGCTTAAAAAATAACTTTCAAAAGTTACACTTTTATCTTTGACTTTTCATAAATGTTTCAGTTAAATTTTCGAATTGTTGCAAACATAAACAGACACTGTGAACTAAATTGAAAACAATAGCAATTAATTGGTTTTTTTGTAAAAAAAAAAAAAACTAATAGTCAAAAACTTAAATGTCTAAAACACATATTTAAGCTACAAATACATACATAAAATTAAATTAGAAAAAAAATGTTATCTTTGAATATAAAAAATGAATCTATTGACTTGTGGTTTGTTGTTTCAGAGGAATTGCAATTAAGGATCCAAGTGCCCCACATGGAGTCCAATTACTCATAGAAGATTATCCATATGCTGTTGATGGACTTGAGATTTGGACAGCAATTAAAACATGGGTACAAGATTATTGCTCTTTTTACTACCAGAAAAATGAAATGATTTGTGATGATCAAGAGCTCCAATCTTGGTGGAAAGAGCTTAGAGAGAAAGGTCATGAAGATAAGAAAAATGAAACATGGTGGCCAAACATGCAAACTCTTGAGGAACTAGTTGAAATATGTACTATCATCATATGGATTTCTTCCGCTCTTCATGCTGCAGTTAATTTTGGACAATATTCTTACGGTGGTTTTTTTCCGAACCGACCAACAATCAGTACGAGATTGTTGCCTGATGAAGGAACTCCAGATTATGAAGAGCTTGAGTTGCATCCCGAGAAGGCTTTCTTGAAAACAATTTCTTCTCAACGGAAAGAGAGCATTTCAGTTGTGTCAATAATTGCGATTTTGTCAATGCATTTTTCTGATGAGGTGTATTTAGGGGAAAGAAGCAATCCAGAGTGGACATGGGATAAGGAAGCTTTGGAAGCATTTGATAAGTTTAGAGGAAGATTAAAGGAAATCGAGGAGAGGATTCTGAAAAGAAACAAAGATCCTAAGTTGAAGAATAGAGTTGGACCTGTAAATGTACCCTACACTCTACTCTTTCCAACTAGCACCAATGGCCTCACAGGCAAAGGAATTCCTAATAGCATTTCCATTTGA

mRNA sequence

ATGAAGGAAAACAGGCTGTATATATTGGATCACCATGATTCAATAATTCCATTTCTAAGAAGAATAAATTCATTAAATTCCACAAAAACATATGCCACAAGAACAATTCTGTTCCTAAAAGATGATGGGACTTTGAAGCCTTTGGCAATTGAATTAAGCTTACCCCACCCAGATGGAGACAAATTTGGTGCCATTAGCAGAGTAGTTTTGGCAGCTAAAGAAGGAGTTGATGCCTCAATTTGGCAATTGGCAAAGGCTTATGTTGCTGTTAATGATACTGGCCACCATCAACTTGTTAGTCACTGGTTGAACACCCATGCAGTAATTGAGCCATTTGTGATTGCAACCAACAGACAATTGAGTGTTCTTCATCCCATTCACAAGTTGCTAGTGCCTCATTTTAAGGATACCATGAAAATCAATGCTCTTGCAAGGGAATCTCTTATTAGTGCTAATGGCATCATTGAGAGAACTCATTATCCAGCTAAATATTCCATGGAGATGTCTTCTTTTGCTTACAAAACTTGGGTCTTCCCTCAACAAGCTCTTCCTGCTGATTTGATTGAGAGAGGAATTGCAATTAAGGATCCAAGTGCCCCACATGGAGTCCAATTACTCATAGAAGATTATCCATATGCTGTTGATGGACTTGAGATTTGGACAGCAATTAAAACATGGGTACAAGATTATTGCTCTTTTTACTACCAGAAAAATGAAATGATTTGTGATGATCAAGAGCTCCAATCTTGGTGGAAAGAGCTTAGAGAGAAAGGTCATGAAGATAAGAAAAATGAAACATGGTGGCCAAACATGCAAACTCTTGAGGAACTAGTTGAAATATGTACTATCATCATATGGATTTCTTCCGCTCTTCATGCTGCAGTTAATTTTGGACAATATTCTTACGGTGGTTTTTTTCCGAACCGACCAACAATCAGTACGAGATTGTTGCCTGATGAAGGAACTCCAGATTATGAAGAGCTTGAGTTGCATCCCGAGAAGGCTTTCTTGAAAACAATTTCTTCTCAACGGAAAGAGAGCATTTCAGTTGTGTCAATAATTGCGATTTTGTCAATGCATTTTTCTGATGAGGTGTATTTAGGGGAAAGAAGCAATCCAGAGTGGACATGGGATAAGGAAGCTTTGGAAGCATTTGATAAGTTTAGAGGAAGATTAAAGGAAATCGAGGAGAGGATTCTGAAAAGAAACAAAGATCCTAAGTTGAAGAATAGAGTTGGACCTGTAAATGTACCCTACACTCTACTCTTTCCAACTAGCACCAATGGCCTCACAGGCAAAGGAATTCCTAATAGCATTTCCATTTGA

Coding sequence (CDS)

ATGAAGGAAAACAGGCTGTATATATTGGATCACCATGATTCAATAATTCCATTTCTAAGAAGAATAAATTCATTAAATTCCACAAAAACATATGCCACAAGAACAATTCTGTTCCTAAAAGATGATGGGACTTTGAAGCCTTTGGCAATTGAATTAAGCTTACCCCACCCAGATGGAGACAAATTTGGTGCCATTAGCAGAGTAGTTTTGGCAGCTAAAGAAGGAGTTGATGCCTCAATTTGGCAATTGGCAAAGGCTTATGTTGCTGTTAATGATACTGGCCACCATCAACTTGTTAGTCACTGGTTGAACACCCATGCAGTAATTGAGCCATTTGTGATTGCAACCAACAGACAATTGAGTGTTCTTCATCCCATTCACAAGTTGCTAGTGCCTCATTTTAAGGATACCATGAAAATCAATGCTCTTGCAAGGGAATCTCTTATTAGTGCTAATGGCATCATTGAGAGAACTCATTATCCAGCTAAATATTCCATGGAGATGTCTTCTTTTGCTTACAAAACTTGGGTCTTCCCTCAACAAGCTCTTCCTGCTGATTTGATTGAGAGAGGAATTGCAATTAAGGATCCAAGTGCCCCACATGGAGTCCAATTACTCATAGAAGATTATCCATATGCTGTTGATGGACTTGAGATTTGGACAGCAATTAAAACATGGGTACAAGATTATTGCTCTTTTTACTACCAGAAAAATGAAATGATTTGTGATGATCAAGAGCTCCAATCTTGGTGGAAAGAGCTTAGAGAGAAAGGTCATGAAGATAAGAAAAATGAAACATGGTGGCCAAACATGCAAACTCTTGAGGAACTAGTTGAAATATGTACTATCATCATATGGATTTCTTCCGCTCTTCATGCTGCAGTTAATTTTGGACAATATTCTTACGGTGGTTTTTTTCCGAACCGACCAACAATCAGTACGAGATTGTTGCCTGATGAAGGAACTCCAGATTATGAAGAGCTTGAGTTGCATCCCGAGAAGGCTTTCTTGAAAACAATTTCTTCTCAACGGAAAGAGAGCATTTCAGTTGTGTCAATAATTGCGATTTTGTCAATGCATTTTTCTGATGAGGTGTATTTAGGGGAAAGAAGCAATCCAGAGTGGACATGGGATAAGGAAGCTTTGGAAGCATTTGATAAGTTTAGAGGAAGATTAAAGGAAATCGAGGAGAGGATTCTGAAAAGAAACAAAGATCCTAAGTTGAAGAATAGAGTTGGACCTGTAAATGTACCCTACACTCTACTCTTTCCAACTAGCACCAATGGCCTCACAGGCAAAGGAATTCCTAATAGCATTTCCATTTGA

Protein sequence

MKENRLYILDHHDSIIPFLRRINSLNSTKTYATRTILFLKDDGTLKPLAIELSLPHPDGDKFGAISRVVLAAKEGVDASIWQLAKAYVAVNDTGHHQLVSHWLNTHAVIEPFVIATNRQLSVLHPIHKLLVPHFKDTMKINALARESLISANGIIERTHYPAKYSMEMSSFAYKTWVFPQQALPADLIERGIAIKDPSAPHGVQLLIEDYPYAVDGLEIWTAIKTWVQDYCSFYYQKNEMICDDQELQSWWKELREKGHEDKKNETWWPNMQTLEELVEICTIIIWISSALHAAVNFGQYSYGGFFPNRPTISTRLLPDEGTPDYEELELHPEKAFLKTISSQRKESISVVSIIAILSMHFSDEVYLGERSNPEWTWDKEALEAFDKFRGRLKEIEERILKRNKDPKLKNRVGPVNVPYTLLFPTSTNGLTGKGIPNSISI
Homology
BLAST of BhiUN32G25 vs. TAIR 10
Match: AT3G22400.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 609.0 bits (1569), Expect = 3.0e-174
Identity = 282/446 (63.23%), Postives = 368/446 (82.51%), Query Frame = 0

Query: 1   MKENRLYILDHHDSIIPFLRRINSLNSTKTYATRTILFLKDDGTLKPLAIELSLPHPDGD 60
           +++N+LYILDHHD+++P+L RINS N TKTYATRT+L L+ DGTLKPLAIELSLPH  G+
Sbjct: 443 LEQNKLYILDHHDALMPYLTRINSTN-TKTYATRTLLLLQADGTLKPLAIELSLPHAQGE 502

Query: 61  KFGAISRVVLAAKEGVDASIWQLAKAYVAVNDTGHHQLVSHWLNTHAVIEPFVIATNRQL 120
            +G++S+V   A++GV+ S+WQLAKAY AVND+G+HQL+SHWL THAVIEPF+IA+NRQL
Sbjct: 503 SYGSVSKVFTPAEKGVEGSVWQLAKAYAAVNDSGYHQLISHWLQTHAVIEPFIIASNRQL 562

Query: 121 SVLHPIHKLLVPHFKDTMKINALARESLISANGIIERTHYPAKYSMEMSSFAYKTWVFPQ 180
           SV+HPIHKLL PHF+DTM INALAR  LI+++G++ERT +P++Y+MEMSS  YK WVF +
Sbjct: 563 SVVHPIHKLLHPHFRDTMNINALARHVLINSDGVLERTVFPSRYAMEMSSSIYKNWVFTE 622

Query: 181 QALPADLIERGIAIKDPSAPHGVQLLIEDYPYAVDGLEIWTAIKTWVQDYCSFYYQKNEM 240
           QALP DL++RG+A++DP++ +GV+LLIEDYP+AVDGLEIW+AIKTWV +YC+FYY  ++ 
Sbjct: 623 QALPKDLLKRGVAVEDPNSDNGVKLLIEDYPFAVDGLEIWSAIKTWVTEYCTFYYNNDKT 682

Query: 241 ICDDQELQSWWKELREKGHEDKKNETWWPNMQTLEELVEICTIIIWISSALHAAVNFGQY 300
           +  D E+QSWW ELR KGH DK++E+WWP+MQT ++L+E CTIIIWI+SALHAAVNFGQY
Sbjct: 683 VQTDTEIQSWWTELRTKGHGDKRHESWWPSMQTRDDLIETCTIIIWIASALHAAVNFGQY 742

Query: 301 SYGGFFPNRPTISTRLLPDEGTPDYEELELHPEKAFLKTISSQRKESISVVSIIAILSMH 360
            Y GF PNRPT+S R +P+ GT +Y ELE   + AFLKTI+ Q  +++  +SII ILSMH
Sbjct: 743 PYAGFLPNRPTVSRRFMPEPGTDEYAELEEDADVAFLKTITPQ-LQTLLGISIIEILSMH 802

Query: 361 FSDEVYLGERSNPEWTWDKEALEAFDKFRGRLKEIEERILKRNKDPKLKNRVGPVNVPYT 420
            +DE+YLG+R +P WT D E LEAF +F   L+ IE  I++RN D + KNR GPVN+PYT
Sbjct: 803 STDEIYLGQRDSPNWTADDEPLEAFKRFGKELELIENNIIRRNNDKRFKNRTGPVNIPYT 862

Query: 421 LLFPTSTN-----GLTGKGIPNSISI 442
           LL+P +T+     G+TGKGIPNS+SI
Sbjct: 863 LLYPNTTDYTREGGITGKGIPNSVSI 886

BLAST of BhiUN32G25 vs. TAIR 10
Match: AT1G55020.1 (lipoxygenase 1 )

HSP 1 Score: 594.3 bits (1531), Expect = 7.7e-170
Identity = 286/442 (64.71%), Postives = 361/442 (81.67%), Query Frame = 0

Query: 1   MKENRLYILDHHDSIIPFLRRINSLNSTKTYATRTILFLKDDGTLKPLAIELSLPHPDGD 60
           +++ RL+ILDHHD+++P+L R+N+  +TKTYA+RT+LFLKDDGTLKPL IELSLPHP+GD
Sbjct: 421 LEKERLFILDHHDTLMPYLGRVNT-TTTKTYASRTLLFLKDDGTLKPLVIELSLPHPNGD 480

Query: 61  KFGAISRVVLAAKEGVDASIWQLAKAYVAVNDTGHHQLVSHWLNTHAVIEPFVIATNRQL 120
           KFGA+S V     EGV  S+WQLAKA+V VND+G+HQL+SHW+ THA IEPFVIATNRQL
Sbjct: 481 KFGAVSEVYTPG-EGVYDSLWQLAKAFVGVNDSGNHQLISHWMQTHASIEPFVIATNRQL 540

Query: 121 SVLHPIHKLLVPHFKDTMKINALARESLISANGIIERTHYPAKYSMEMSSFAYKT-WVFP 180
           SVLHP+ KLL PHF+DTM INALAR+ LI+  GI E T +P+KY+MEMSSF YK  W FP
Sbjct: 541 SVLHPVFKLLEPHFRDTMNINALARQILINGGGIFEITVFPSKYAMEMSSFIYKNHWTFP 600

Query: 181 QQALPADLIERGIAIKDPSAPHGVQLLIEDYPYAVDGLEIWTAIKTWVQDYCSFYYQKNE 240
            QALPA+L +RG+A++DP APHG++L I+DYPYAVDGLE+W AI++WV+DY   +Y+  E
Sbjct: 601 DQALPAELKKRGMAVEDPEAPHGLRLRIKDYPYAVDGLEVWYAIESWVRDYIFLFYKIEE 660

Query: 241 MICDDQELQSWWKELREKGHEDKKNETWWPNMQTLEELVEICTIIIWISSALHAAVNFGQ 300
            I  D ELQ+WWKE+RE+GH DKK+E WWP MQT EELVE CTIIIW++SALHAAVNFGQ
Sbjct: 661 DIQTDTELQAWWKEVREEGHGDKKSEPWWPKMQTREELVESCTIIIWVASALHAAVNFGQ 720

Query: 301 YSYGGFFPNRPTISTRLLPDEGTPDYEELELHPEKAFLKTISSQRKESISVVSIIAILSM 360
           Y   G+ PNRPTIS + +P E TP++EELE +P+K FLKTI++Q  +++  +S+I ILS 
Sbjct: 721 YPVAGYLPNRPTISRQYMPKENTPEFEELEKNPDKVFLKTITAQ-LQTLLGISLIEILST 780

Query: 361 HFSDEVYLGERSNPEWTWDKEALEAFDKFRGRLKEIEERILKRNKDPKLKNRVGPVNVPY 420
           H SDEVYLG+R + EW  +KEALEAF+KF  ++KEIE+ I +RN D  LKNR G V +PY
Sbjct: 781 HSSDEVYLGQRDSKEWAAEKEALEAFEKFGEKVKEIEKNIDERNDDETLKNRTGLVKMPY 840

Query: 421 TLLFPTSTNGLTGKGIPNSISI 442
           TLLFP+S  G+TG+GIPNS+SI
Sbjct: 841 TLLFPSSEGGVTGRGIPNSVSI 859

BLAST of BhiUN32G25 vs. TAIR 10
Match: AT1G17420.1 (lipoxygenase 3 )

HSP 1 Score: 457.6 bits (1176), Expect = 1.1e-128
Identity = 232/443 (52.37%), Postives = 304/443 (68.62%), Query Frame = 0

Query: 1   MKENRLYILDHHDSIIPFLRRINSLNSTKTYATRTILFLKDDGTLKPLAIELSLPHPDGD 60
           ++ENRLY+LD+HD  +PFL RIN+L+  K YATRTI FL   GTLKP+AIELSLP P G 
Sbjct: 480 LEENRLYMLDYHDIFLPFLDRINALDGRKAYATRTIFFLTRLGTLKPVAIELSLP-PHGP 539

Query: 61  KFGAISRVVLAAKEGVDASIWQLAKAYVAVNDTGHHQLVSHWLNTHAVIEPFVIATNRQL 120
           K  +  RV+    +     +WQLAKA+V+ ND G HQLV+HWL THA +EPF++A +RQL
Sbjct: 540 KHRS-KRVLTPPVDATSNWMWQLAKAHVSSNDAGVHQLVNHWLRTHACLEPFILAAHRQL 599

Query: 121 SVLHPIHKLLVPHFKDTMKINALARESLISANGIIERTHYPAKYSMEMSSFAYK-TWVFP 180
           S +HPI KLL PH + T++INALAR+SLISA+G+IE       Y MEMS+ AYK +W F 
Sbjct: 600 SAMHPIFKLLDPHMRYTLEINALARQSLISADGVIEGGFTAGAYGMEMSAAAYKSSWRFD 659

Query: 181 QQALPADLIERGIAIKDPSAPHGVQLLIEDYPYAVDGLEIWTAIKTWVQDYCSFYYQKNE 240
            + LPADLI RG+AI D + PHG++LLIEDYPYA DGL +W+AI+TWV+ Y   YY    
Sbjct: 660 MEGLPADLIRRGMAIPDATQPHGLKLLIEDYPYANDGLLLWSAIQTWVRTYVERYYPNPN 719

Query: 241 MICDDQELQSWWKELREKGHEDKKNETWWPNMQTLEELVEICTIIIWISSALHAAVNFGQ 300
           +I  D ELQSW+ E    GH D ++  WWP + T+++LV I T +IW++SA HAA+NFGQ
Sbjct: 720 LIKTDSELQSWYSESINVGHADLRDADWWPELSTVDDLVSILTTLIWLASAQHAALNFGQ 779

Query: 301 YSYGGFFPNRPTISTRLLPDEGTPDYEELELHPEKAFLKTISSQRKESISVVSIIAILSM 360
           Y YGG+ PNRP +  RL+PDE  P+Y     HPEK +  ++ S  + S   ++++  LS 
Sbjct: 780 YPYGGYVPNRPPLMRRLIPDESDPEYASFISHPEKYYFSSMPSLAQTS-KFMAVVDTLST 839

Query: 361 HFSDEVYLGERSNPE-WTWDKEALEAFDKFRGRLKEIEERILKRNKDPKLKNRVGPVNVP 420
           H  DE Y+GER  P  WT D E +EAF  F   +  IE+ I KRN DP  +NR G   +P
Sbjct: 840 HSPDEEYIGERQQPSIWTGDAEIVEAFYGFAAEIGRIEKEIEKRNADPDRRNRCGAGVLP 899

Query: 421 YTLLFPTSTNGLTGKGIPNSISI 442
           Y LL P+S  G+T +G+PNS+SI
Sbjct: 900 YELLVPSSEPGVTCRGVPNSVSI 919

BLAST of BhiUN32G25 vs. TAIR 10
Match: AT1G72520.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 445.3 bits (1144), Expect = 5.8e-125
Identity = 219/443 (49.44%), Postives = 302/443 (68.17%), Query Frame = 0

Query: 1   MKENRLYILDHHDSIIPFLRRINSLNSTKTYATRTILFLKDDGTLKPLAIELSLPHPDGD 60
           ++ NRL+++D+HD  +PFL RIN+L+  K YATRTILFL   GTLKP+AIELSLP     
Sbjct: 486 LETNRLFMVDYHDIYLPFLDRINALDGRKAYATRTILFLTRLGTLKPIAIELSLPSQSSS 545

Query: 61  KFGAISRVVLAAKEGVDASIWQLAKAYVAVNDTGHHQLVSHWLNTHAVIEPFVIATNRQL 120
              +  RVV    +     +WQLAKA+V  ND G HQLV+HWL THA +EPF++A +RQL
Sbjct: 546 NQKS-KRVVTPPVDATSNWMWQLAKAHVGSNDAGVHQLVNHWLRTHACLEPFILAAHRQL 605

Query: 121 SVLHPIHKLLVPHFKDTMKINALARESLISANGIIERTHYPAKYSMEMSSFAYKT-WVFP 180
           S +HPI KLL PH + T++INA+AR++LISA+G+IE      +Y +E+SS AYK  W F 
Sbjct: 606 SAMHPIFKLLDPHMRYTLEINAVARQTLISADGVIESCFTAGQYGLEISSAAYKNKWRFD 665

Query: 181 QQALPADLIERGIAIKDPSAPHGVQLLIEDYPYAVDGLEIWTAIKTWVQDYCSFYYQKNE 240
            + LPADLI RG+A+ DP+ PHG++LL+EDYPYA DGL +W+AI+TWV+ Y   YY  + 
Sbjct: 666 MEGLPADLIRRGMAVPDPTQPHGLKLLVEDYPYANDGLLLWSAIQTWVRTYVERYYANSN 725

Query: 241 MICDDQELQSWWKELREKGHEDKKNETWWPNMQTLEELVEICTIIIWISSALHAAVNFGQ 300
           +I  D ELQ+W+ E    GH D ++  WWP + T+E+LV + T IIW++SA HAA+NFGQ
Sbjct: 726 LIQTDTELQAWYSESINVGHADHRDAEWWPKLSTVEDLVSVITTIIWLASAQHAALNFGQ 785

Query: 301 YSYGGFFPNRPTISTRLLPDEGTPDYEELELHPEKAFLKTISSQRKESISVVSIIAILSM 360
           Y YGG+ PNRP +  RL+PDE  P++      P+K F  ++ S   ++   ++++  LS 
Sbjct: 786 YPYGGYVPNRPPLMRRLIPDESDPEFTSFIEDPQKYFFSSMPS-LLQTTKFMAVVDTLST 845

Query: 361 HFSDEVYLGERSNPE-WTWDKEALEAFDKFRGRLKEIEERILKRNKDPKLKNRVGPVNVP 420
           H  DE Y+GER  P  WT D E ++AF  F   +  IE+ I KRN+DP  +NR G   +P
Sbjct: 846 HSPDEEYIGERQQPSIWTGDAEIVDAFYGFSAEIGRIEKEIDKRNRDPSRRNRCGAGVLP 905

Query: 421 YTLLFPTSTNGLTGKGIPNSISI 442
           Y L+ P+S  G+T +G+PNS+SI
Sbjct: 906 YELMAPSSEPGVTCRGVPNSVSI 926

BLAST of BhiUN32G25 vs. TAIR 10
Match: AT1G67560.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 439.1 bits (1128), Expect = 4.2e-123
Identity = 226/449 (50.33%), Postives = 309/449 (68.82%), Query Frame = 0

Query: 1   MKENRLYILDHHDSIIPFLRRINSL--NSTKTYATRTILFLKDDGTLKPLAIELSLPHPD 60
           ++E RL+++D+HD ++PF+ +INS+  +  KTYA+RTI F   +G L+PLAIELSLP   
Sbjct: 476 LEEKRLFLVDYHDILLPFVEKINSIKEDPRKTYASRTIFFYSKNGALRPLAIELSLPPT- 535

Query: 61  GDKFGAISRVVLAAKEGVDAS---IWQLAKAYVAVNDTGHHQLVSHWLNTHAVIEPFVIA 120
                A S        G DA+   IW+LAKA+V  ND G HQLV+HWL THA +EP++IA
Sbjct: 536 -----AESENKFVYTHGHDATTHWIWKLAKAHVCSNDAGVHQLVNHWLRTHASMEPYIIA 595

Query: 121 TNRQLSVLHPIHKLLVPHFKDTMKINALARESLISANGIIERTHYPAKYSMEMSSFAYKT 180
           TNRQLS +HP++KLL PH + T++INA AR+SLI+  GIIE    P KY+ME+SS AYK+
Sbjct: 596 TNRQLSTMHPVYKLLHPHMRYTLEINARARKSLINGGGIIESCFTPGKYAMELSSAAYKS 655

Query: 181 -WVFPQQALPADLIERGIAIKDPSAPHGVQLLIEDYPYAVDGLEIWTAIKTWVQDYCSFY 240
            W F  + LPADL+ RG+A +D SA  GV+L+I+DYPYA DGL IW AIK  V+ Y   +
Sbjct: 656 MWRFDMEGLPADLVRRGMAEEDSSAECGVRLVIDDYPYAADGLLIWKAIKDLVESYVKHF 715

Query: 241 YQKNEMICDDQELQSWWKELREKGHEDKKNETWWPNMQTLEELVEICTIIIWISSALHAA 300
           Y  ++ I  D ELQ+WW E++ KGH DKK+E WWP + T ++L +I T +IWI+S  HAA
Sbjct: 716 YSDSKSITSDLELQAWWDEIKNKGHYDKKDEPWWPKLNTTQDLSQILTNMIWIASGQHAA 775

Query: 301 VNFGQYSYGGFFPNRPTISTRLLPDEGTPDYEELELHPEKAFLKTISSQRKESISVVSII 360
           +NFGQY +GG+ PNRPT+  +L+P E  PDYE    +P+ +FL ++ +Q  ++  V+++ 
Sbjct: 776 INFGQYPFGGYVPNRPTLLRKLIPQETDPDYEMFMRNPQYSFLGSLPTQ-LQATKVMAVQ 835

Query: 361 AILSMHFSDEVYLGERSNPE--WTWDKEALEAFDKFRGRLKEIEERILKRNKDPKLKNRV 420
             LS H  DE YL E    +  W  D++ ++ F+KF   L +IE+ I +RNKD KLKNR 
Sbjct: 836 ETLSTHSPDEEYLIELREVQRHWFQDEQVVKYFNKFSEELVKIEKTINERNKDKKLKNRT 895

Query: 421 GPVNVPYTLLFPTSTNGLTGKGIPNSISI 442
           G    PY LL PTS +G+TG+GIPNSISI
Sbjct: 896 GAGMPPYELLLPTSPHGVTGRGIPNSISI 917

BLAST of BhiUN32G25 vs. ExPASy Swiss-Prot
Match: Q41238 (Linoleate 9S-lipoxygenase 6 (Fragment) OS=Solanum tuberosum OX=4113 GN=LOX1.6 PE=1 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 1.1e-184
Identity = 304/441 (68.93%), Postives = 376/441 (85.26%), Query Frame = 0

Query: 1   MKENRLYILDHHDSIIPFLRRINSLNSTKTYATRTILFLKDDGTLKPLAIELSLPHPDGD 60
           M  N+L+IL+HHD +IP+LRRIN+  +TKTYA+RT+LFL+D+G+LKPLAIELSLPHPDGD
Sbjct: 419 MNNNKLFILNHHDVLIPYLRRINT-TTTKTYASRTLLFLQDNGSLKPLAIELSLPHPDGD 478

Query: 61  KFGAISRVVLAAKEGVDASIWQLAKAYVAVNDTGHHQLVSHWLNTHAVIEPFVIATNRQL 120
           +FG IS+V   + +GV++SIWQLAKAYVAVND+G HQL+SHWLNTHAVIEPFVIATNRQL
Sbjct: 479 QFGVISKVYTPSDQGVESSIWQLAKAYVAVNDSGVHQLISHWLNTHAVIEPFVIATNRQL 538

Query: 121 SVLHPIHKLLVPHFKDTMKINALARESLISANGIIERTHYPAKYSMEMSSFAYKTWVFPQ 180
           SVLHPIHKLL PHF+DTM INA+AR+ LI+A G++E T +P+K++MEMS+  YK WVFP 
Sbjct: 539 SVLHPIHKLLYPHFRDTMNINAMARQILINAGGVLESTVFPSKFAMEMSAVVYKDWVFPD 598

Query: 181 QALPADLIERGIAIKDPSAPHGVQLLIEDYPYAVDGLEIWTAIKTWVQDYCSFYYQKNEM 240
           QALPADL++RG+A++D S+PHGV+LLIEDYPYAVDGLEIW+AIK+WV DYCSFYY  +E 
Sbjct: 599 QALPADLVKRGVAVEDSSSPHGVRLLIEDYPYAVDGLEIWSAIKSWVTDYCSFYYGSDEE 658

Query: 241 ICDDQELQSWWKELREKGHEDKKNETWWPNMQTLEELVEICTIIIWISSALHAAVNFGQY 300
           I  D ELQ+WWKELRE GH DKKNE WWP M+T +EL++ CT IIWI+SALHAAVNFGQY
Sbjct: 659 ILKDNELQAWWKELREVGHGDKKNEPWWPEMETPQELIDSCTTIIWIASALHAAVNFGQY 718

Query: 301 SYGGFFPNRPTISTRLLPDEGTPDYEELELHPEKAFLKTISSQRKESISVVSIIAILSMH 360
            Y G+ PNRPT+S R +P+ GTP+YEEL+ +P+KAFLKTI++Q  +++  VS+I ILS H
Sbjct: 719 PYAGYLPNRPTVSRRFMPEPGTPEYEELKKNPDKAFLKTITAQ-LQTLLGVSLIEILSRH 778

Query: 361 FSDEVYLGERSNPEWTWDKEALEAFDKFRGRLKEIEERILKRNKDPKLKNRVGPVNVPYT 420
            +DE+YLG+R +PEWT DKE L AFDKF  +L +IE++I++RN D  L NR GPVN PYT
Sbjct: 779 TTDEIYLGQRESPEWTKDKEPLAAFDKFGKKLTDIEKQIIQRNGDNILTNRSGPVNAPYT 838

Query: 421 LLFPTSTNGLTGKGIPNSISI 442
           LLFPTS  GLTGKGIPNS+SI
Sbjct: 839 LLFPTSEGGLTGKGIPNSVSI 857

BLAST of BhiUN32G25 vs. ExPASy Swiss-Prot
Match: Q43191 (Probable linoleate 9S-lipoxygenase 5 OS=Solanum tuberosum OX=4113 GN=LOX1.5 PE=2 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 3.2e-184
Identity = 300/441 (68.03%), Postives = 378/441 (85.71%), Query Frame = 0

Query: 1   MKENRLYILDHHDSIIPFLRRINSLNSTKTYATRTILFLKDDGTLKPLAIELSLPHPDGD 60
           +K NRLYIL+HHD ++P++RRIN+ N TK YA+RT+LFL+DDGT+KP+AIELSLPHPDGD
Sbjct: 424 IKTNRLYILNHHDILMPYVRRINTTN-TKLYASRTLLFLQDDGTMKPVAIELSLPHPDGD 483

Query: 61  KFGAISRVVLAAKEGVDASIWQLAKAYVAVNDTGHHQLVSHWLNTHAVIEPFVIATNRQL 120
           + GA+S+V   A +GV+ SIWQLAKAYVAVND+G HQL+SHWLNTHA IEPFVIATNRQL
Sbjct: 484 ELGAVSKVYTPADQGVEGSIWQLAKAYVAVNDSGVHQLISHWLNTHAAIEPFVIATNRQL 543

Query: 121 SVLHPIHKLLVPHFKDTMKINALARESLISANGIIERTHYPAKYSMEMSSFAYKTWVFPQ 180
           SVLHPIHKLL PHF+DTM INALAR+ LI+A G++E T +PAKY+MEMS+  YK+WVFP+
Sbjct: 544 SVLHPIHKLLHPHFRDTMNINALARQILINAGGVLEMTVFPAKYAMEMSAVVYKSWVFPE 603

Query: 181 QALPADLIERGIAIKDPSAPHGVQLLIEDYPYAVDGLEIWTAIKTWVQDYCSFYYQKNEM 240
           QALPADLI+RG+A++D S+PHGV+LLI+DYPYAVDGLEIW+AIK+WV +YC+FYY+ +E+
Sbjct: 604 QALPADLIKRGVAVEDSSSPHGVRLLIQDYPYAVDGLEIWSAIKSWVTEYCNFYYKSDEL 663

Query: 241 ICDDQELQSWWKELREKGHEDKKNETWWPNMQTLEELVEICTIIIWISSALHAAVNFGQY 300
           +  D ELQ+WWKELRE+GH DKK+E WWP MQT +EL + CTIIIWI+SALHAAVNFGQY
Sbjct: 664 VLKDNELQAWWKELREEGHGDKKDEPWWPKMQTRQELKDSCTIIIWIASALHAAVNFGQY 723

Query: 301 SYGGFFPNRPTISTRLLPDEGTPDYEELELHPEKAFLKTISSQRKESISVVSIIAILSMH 360
            Y G+ PNRPT+S R +P+ GTP+YEEL+ +P+KA+LKTI+ Q  +++  +S+I ILS H
Sbjct: 724 PYAGYLPNRPTLSRRFMPEPGTPEYEELKTNPDKAYLKTITPQ-LQTLLGISLIEILSRH 783

Query: 361 FSDEVYLGERSNPEWTWDKEALEAFDKFRGRLKEIEERILKRNKDPKLKNRVGPVNVPYT 420
            SDE+YLG+R + EWT D+E + AF++F  +L EIE++I++ N D K KNR GPVNVPYT
Sbjct: 784 ASDEIYLGQRDSSEWTKDQEPIAAFERFGKKLSEIEDQIIQMNGDKKWKNRSGPVNVPYT 843

Query: 421 LLFPTSTNGLTGKGIPNSISI 442
           LLFPTS  GLTGKGIPNS+SI
Sbjct: 844 LLFPTSEQGLTGKGIPNSVSI 862

BLAST of BhiUN32G25 vs. ExPASy Swiss-Prot
Match: Q43190 (Probable linoleate 9S-lipoxygenase 4 OS=Solanum tuberosum OX=4113 GN=LOX1.4 PE=2 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 7.0e-184
Identity = 303/441 (68.71%), Postives = 375/441 (85.03%), Query Frame = 0

Query: 1   MKENRLYILDHHDSIIPFLRRINSLNSTKTYATRTILFLKDDGTLKPLAIELSLPHPDGD 60
           M  N+L+IL+HHD +IP+LRRIN+  +TKTYA+RT+LFL+D+G+LKPLAIELSLPHPDGD
Sbjct: 422 MNNNKLFILNHHDVLIPYLRRINT-TTTKTYASRTLLFLQDNGSLKPLAIELSLPHPDGD 481

Query: 61  KFGAISRVVLAAKEGVDASIWQLAKAYVAVNDTGHHQLVSHWLNTHAVIEPFVIATNRQL 120
           +FG  S+V   + +GV++SIWQLAKAYVAVND+G HQL+SHWLNTHAVIEPFVIATNRQL
Sbjct: 482 QFGVTSKVYTPSDQGVESSIWQLAKAYVAVNDSGVHQLISHWLNTHAVIEPFVIATNRQL 541

Query: 121 SVLHPIHKLLVPHFKDTMKINALARESLISANGIIERTHYPAKYSMEMSSFAYKTWVFPQ 180
           SVLHPIHKLL PHF+DTM INA+AR+ LI+A G++E T +P+K++MEMS+  YK WVFP 
Sbjct: 542 SVLHPIHKLLYPHFRDTMNINAMARQILINAGGVLESTVFPSKFAMEMSAVVYKDWVFPD 601

Query: 181 QALPADLIERGIAIKDPSAPHGVQLLIEDYPYAVDGLEIWTAIKTWVQDYCSFYYQKNEM 240
           QALPADL++RG+A++D S+PHGV+LLIEDYPYAVDGLEIW+AIK+WV DYCSFYY  +E 
Sbjct: 602 QALPADLVKRGVAVEDSSSPHGVRLLIEDYPYAVDGLEIWSAIKSWVTDYCSFYYGSDEE 661

Query: 241 ICDDQELQSWWKELREKGHEDKKNETWWPNMQTLEELVEICTIIIWISSALHAAVNFGQY 300
           I  D ELQ+WWKELRE GH DKKNE WWP M+T +EL++ CT IIWI+SALHAAVNFGQY
Sbjct: 662 ILKDNELQAWWKELREVGHGDKKNEPWWPEMETPQELIDSCTTIIWIASALHAAVNFGQY 721

Query: 301 SYGGFFPNRPTISTRLLPDEGTPDYEELELHPEKAFLKTISSQRKESISVVSIIAILSMH 360
            Y G+ PNRPT+S R +P+ GTP+YEEL+ +P+KAFLKTI++Q  +++  VS+I ILS H
Sbjct: 722 PYAGYLPNRPTVSRRFMPEPGTPEYEELKKNPDKAFLKTITAQ-LQTLLGVSLIEILSRH 781

Query: 361 FSDEVYLGERSNPEWTWDKEALEAFDKFRGRLKEIEERILKRNKDPKLKNRVGPVNVPYT 420
            +DE+YLG+R +PEWT DKE L AFDKF  +L +IE++I++RN D  L NR GPVN PYT
Sbjct: 782 TTDEIYLGQRESPEWTKDKEPLAAFDKFGKKLTDIEKQIIQRNGDNILINRSGPVNAPYT 841

Query: 421 LLFPTSTNGLTGKGIPNSISI 442
           LLFPTS  GLTGKGIPNS+SI
Sbjct: 842 LLFPTSEGGLTGKGIPNSVSI 860

BLAST of BhiUN32G25 vs. ExPASy Swiss-Prot
Match: O22507 (Probable linoleate 9S-lipoxygenase 7 OS=Solanum tuberosum OX=4113 GN=LOX1.7 PE=2 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 7.8e-183
Identity = 302/441 (68.48%), Postives = 372/441 (84.35%), Query Frame = 0

Query: 1   MKENRLYILDHHDSIIPFLRRINSLNSTKTYATRTILFLKDDGTLKPLAIELSLPHPDGD 60
           M  N+L+IL+HHD IIP+LRRIN+   TKTYA+RT+LFL+D+G+LKPLAIELSLPHPDGD
Sbjct: 423 MNNNKLFILNHHDVIIPYLRRINT-TITKTYASRTLLFLQDNGSLKPLAIELSLPHPDGD 482

Query: 61  KFGAISRVVLAAKEGVDASIWQLAKAYVAVNDTGHHQLVSHWLNTHAVIEPFVIATNRQL 120
           +FG  S+V   + +GV++SIWQLAKAYVAVNDTG HQL+SHWLNTHAVIEPFVIATNRQL
Sbjct: 483 QFGVTSKVYTPSDQGVESSIWQLAKAYVAVNDTGVHQLISHWLNTHAVIEPFVIATNRQL 542

Query: 121 SVLHPIHKLLVPHFKDTMKINALARESLISANGIIERTHYPAKYSMEMSSFAYKTWVFPQ 180
           SVLHPIHKLL PHF+DTM INA AR+ L++A G++E T + +K++MEMS+  YK WVFP 
Sbjct: 543 SVLHPIHKLLYPHFRDTMNINASARQILVNAGGVLESTVFQSKFAMEMSAVVYKDWVFPD 602

Query: 181 QALPADLIERGIAIKDPSAPHGVQLLIEDYPYAVDGLEIWTAIKTWVQDYCSFYYQKNEM 240
           QALPADL++RG+A++D S+PHGV+LLIEDYPYAVDGLEIW+AIK+WV DYCSFYY  +E 
Sbjct: 603 QALPADLVKRGVAVEDSSSPHGVRLLIEDYPYAVDGLEIWSAIKSWVTDYCSFYYGSDEE 662

Query: 241 ICDDQELQSWWKELREKGHEDKKNETWWPNMQTLEELVEICTIIIWISSALHAAVNFGQY 300
           I  D ELQ+WWKELRE GH DKKNE WWP M+T +EL++ CT IIWI+SALHAAVNFGQY
Sbjct: 663 ILKDNELQAWWKELREVGHGDKKNEPWWPEMKTPQELIDSCTTIIWIASALHAAVNFGQY 722

Query: 301 SYGGFFPNRPTISTRLLPDEGTPDYEELELHPEKAFLKTISSQRKESISVVSIIAILSMH 360
            Y G+ PNRPT+S R +P+ GTPDYEEL+ +P+KAFLKTI++Q  +++  VS++ ILS H
Sbjct: 723 PYAGYLPNRPTVSRRFMPEPGTPDYEELKRNPDKAFLKTITAQ-LQTLLGVSLVEILSRH 782

Query: 361 FSDEVYLGERSNPEWTWDKEALEAFDKFRGRLKEIEERILKRNKDPKLKNRVGPVNVPYT 420
            +DE+YLG+R +PEWT DKE L AFD+F  +L +IE++I++RN D  L NR GPVN PYT
Sbjct: 783 TTDEIYLGQRESPEWTKDKEPLAAFDRFGKKLTDIEKQIIQRNGDNILTNRSGPVNAPYT 842

Query: 421 LLFPTSTNGLTGKGIPNSISI 442
           LLFPTS  GLTGKGIPNS+SI
Sbjct: 843 LLFPTSEGGLTGKGIPNSVSI 861

BLAST of BhiUN32G25 vs. ExPASy Swiss-Prot
Match: O24379 (Linoleate 9S-lipoxygenase 2 OS=Solanum tuberosum OX=4113 GN=LOX1.2 PE=1 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 1.3e-182
Identity = 301/441 (68.25%), Postives = 373/441 (84.58%), Query Frame = 0

Query: 1   MKENRLYILDHHDSIIPFLRRINSLNSTKTYATRTILFLKDDGTLKPLAIELSLPHPDGD 60
           M  N+L+IL+HHD +IP+LRRIN+  +TKTYA+RT+LFL+D+G+LKPLAIELSLPHPDGD
Sbjct: 423 MNNNKLFILNHHDVLIPYLRRINT-TTTKTYASRTLLFLQDNGSLKPLAIELSLPHPDGD 482

Query: 61  KFGAISRVVLAAKEGVDASIWQLAKAYVAVNDTGHHQLVSHWLNTHAVIEPFVIATNRQL 120
           +FG  S+V   + +GV++SIWQLAKAYVAVND+G HQL+SHWLNTHAVIEPFVIATNRQL
Sbjct: 483 QFGVTSKVYTPSDQGVESSIWQLAKAYVAVNDSGVHQLISHWLNTHAVIEPFVIATNRQL 542

Query: 121 SVLHPIHKLLVPHFKDTMKINALARESLISANGIIERTHYPAKYSMEMSSFAYKTWVFPQ 180
           SVLHPIHKLL PHF+DTM INA+AR+ LI+A G++E T + +K++MEMS+  YK WVFP 
Sbjct: 543 SVLHPIHKLLYPHFRDTMNINAMARQILINAGGVLESTVFQSKFAMEMSAVVYKDWVFPD 602

Query: 181 QALPADLIERGIAIKDPSAPHGVQLLIEDYPYAVDGLEIWTAIKTWVQDYCSFYYQKNEM 240
           QALPADL++RG+A++D S+PHGV+LLIEDYPYAVDGLEIW+AIK+WV DYCSFYY  +E 
Sbjct: 603 QALPADLVKRGVAVEDSSSPHGVRLLIEDYPYAVDGLEIWSAIKSWVSDYCSFYYGSDEE 662

Query: 241 ICDDQELQSWWKELREKGHEDKKNETWWPNMQTLEELVEICTIIIWISSALHAAVNFGQY 300
           I  D ELQ+WWKELRE GH DKKNE WWP M+  +EL++ CT IIWI+SALHAAVNFGQY
Sbjct: 663 ILKDNELQAWWKELREVGHGDKKNEPWWPEMERPQELIDSCTTIIWIASALHAAVNFGQY 722

Query: 301 SYGGFFPNRPTISTRLLPDEGTPDYEELELHPEKAFLKTISSQRKESISVVSIIAILSMH 360
            Y G+ PNRPT+S R +P+ GTP+YEEL+ +P+KAFLKTI++Q  +++  VS+I ILS H
Sbjct: 723 PYAGYLPNRPTVSRRFMPEPGTPEYEELKKNPDKAFLKTITAQ-LQTLLGVSLIEILSRH 782

Query: 361 FSDEVYLGERSNPEWTWDKEALEAFDKFRGRLKEIEERILKRNKDPKLKNRVGPVNVPYT 420
            +DE+YLG+R +PEWT DKE L AFDKF  +L +IE++I++RN D  L NR GPVN PYT
Sbjct: 783 TTDEIYLGQRESPEWTKDKEPLAAFDKFGKKLTDIEKQIIQRNGDNILTNRSGPVNAPYT 842

Query: 421 LLFPTSTNGLTGKGIPNSISI 442
           LLFPTS  GLTGKGIPNS+SI
Sbjct: 843 LLFPTSEGGLTGKGIPNSVSI 861

BLAST of BhiUN32G25 vs. NCBI nr
Match: XP_038877029.1 (probable linoleate 9S-lipoxygenase 5 [Benincasa hispida])

HSP 1 Score: 899.0 bits (2322), Expect = 1.6e-257
Identity = 441/441 (100.00%), Postives = 441/441 (100.00%), Query Frame = 0

Query: 1   MKENRLYILDHHDSIIPFLRRINSLNSTKTYATRTILFLKDDGTLKPLAIELSLPHPDGD 60
           MKENRLYILDHHDSIIPFLRRINSLNSTKTYATRTILFLKDDGTLKPLAIELSLPHPDGD
Sbjct: 427 MKENRLYILDHHDSIIPFLRRINSLNSTKTYATRTILFLKDDGTLKPLAIELSLPHPDGD 486

Query: 61  KFGAISRVVLAAKEGVDASIWQLAKAYVAVNDTGHHQLVSHWLNTHAVIEPFVIATNRQL 120
           KFGAISRVVLAAKEGVDASIWQLAKAYVAVNDTGHHQLVSHWLNTHAVIEPFVIATNRQL
Sbjct: 487 KFGAISRVVLAAKEGVDASIWQLAKAYVAVNDTGHHQLVSHWLNTHAVIEPFVIATNRQL 546

Query: 121 SVLHPIHKLLVPHFKDTMKINALARESLISANGIIERTHYPAKYSMEMSSFAYKTWVFPQ 180
           SVLHPIHKLLVPHFKDTMKINALARESLISANGIIERTHYPAKYSMEMSSFAYKTWVFPQ
Sbjct: 547 SVLHPIHKLLVPHFKDTMKINALARESLISANGIIERTHYPAKYSMEMSSFAYKTWVFPQ 606

Query: 181 QALPADLIERGIAIKDPSAPHGVQLLIEDYPYAVDGLEIWTAIKTWVQDYCSFYYQKNEM 240
           QALPADLIERGIAIKDPSAPHGVQLLIEDYPYAVDGLEIWTAIKTWVQDYCSFYYQKNEM
Sbjct: 607 QALPADLIERGIAIKDPSAPHGVQLLIEDYPYAVDGLEIWTAIKTWVQDYCSFYYQKNEM 666

Query: 241 ICDDQELQSWWKELREKGHEDKKNETWWPNMQTLEELVEICTIIIWISSALHAAVNFGQY 300
           ICDDQELQSWWKELREKGHEDKKNETWWPNMQTLEELVEICTIIIWISSALHAAVNFGQY
Sbjct: 667 ICDDQELQSWWKELREKGHEDKKNETWWPNMQTLEELVEICTIIIWISSALHAAVNFGQY 726

Query: 301 SYGGFFPNRPTISTRLLPDEGTPDYEELELHPEKAFLKTISSQRKESISVVSIIAILSMH 360
           SYGGFFPNRPTISTRLLPDEGTPDYEELELHPEKAFLKTISSQRKESISVVSIIAILSMH
Sbjct: 727 SYGGFFPNRPTISTRLLPDEGTPDYEELELHPEKAFLKTISSQRKESISVVSIIAILSMH 786

Query: 361 FSDEVYLGERSNPEWTWDKEALEAFDKFRGRLKEIEERILKRNKDPKLKNRVGPVNVPYT 420
           FSDEVYLGERSNPEWTWDKEALEAFDKFRGRLKEIEERILKRNKDPKLKNRVGPVNVPYT
Sbjct: 787 FSDEVYLGERSNPEWTWDKEALEAFDKFRGRLKEIEERILKRNKDPKLKNRVGPVNVPYT 846

Query: 421 LLFPTSTNGLTGKGIPNSISI 442
           LLFPTSTNGLTGKGIPNSISI
Sbjct: 847 LLFPTSTNGLTGKGIPNSISI 867

BLAST of BhiUN32G25 vs. NCBI nr
Match: XP_022150790.1 (probable linoleate 9S-lipoxygenase 5 [Momordica charantia])

HSP 1 Score: 719.5 bits (1856), Expect = 1.7e-203
Identity = 347/441 (78.68%), Postives = 394/441 (89.34%), Query Frame = 0

Query: 1   MKENRLYILDHHDSIIPFLRRINSLNSTKTYATRTILFLKDDGTLKPLAIELSLPHPDGD 60
           +++N+LYILDHHDS+IP+LRRIN+   TKTYATRT+LFLK+DGTLKPLAIELSLPHP GD
Sbjct: 424 LEKNKLYILDHHDSLIPYLRRINT-TPTKTYATRTLLFLKNDGTLKPLAIELSLPHPQGD 483

Query: 61  KFGAISRVVLAAKEGVDASIWQLAKAYVAVNDTGHHQLVSHWLNTHAVIEPFVIATNRQL 120
           +FG IS+V+L AK GVD SIWQLAKAYVAVNDTG+HQL+SHWLNTHAVIEPFVIATNRQL
Sbjct: 484 QFGVISKVLLPAKGGVDGSIWQLAKAYVAVNDTGYHQLISHWLNTHAVIEPFVIATNRQL 543

Query: 121 SVLHPIHKLLVPHFKDTMKINALARESLISANGIIERTHYPAKYSMEMSSFAYKTWVFPQ 180
           SV+HPIHKLLVPHF+DTM INALAR+SLI+A+GIIE THYPAKYSMEMSSF YK WVFP+
Sbjct: 544 SVVHPIHKLLVPHFRDTMNINALARQSLINADGIIETTHYPAKYSMEMSSFVYKNWVFPE 603

Query: 181 QALPADLIERGIAIKDPSAPHGVQLLIEDYPYAVDGLEIWTAIKTWVQDYCSFYYQKNEM 240
           QALPADLI+RGIAI+DPS+PHG++LLIEDYPYAVDGLEIWTAIKTWV DYCSFYY+ NE 
Sbjct: 604 QALPADLIKRGIAIQDPSSPHGLRLLIEDYPYAVDGLEIWTAIKTWVTDYCSFYYKDNET 663

Query: 241 ICDDQELQSWWKELREKGHEDKKNETWWPNMQTLEELVEICTIIIWISSALHAAVNFGQY 300
           + +D ELQSWWKELREKGH D K+E WWP MQT+++L++ CTIIIWISSALHAAVNFGQY
Sbjct: 664 LHNDVELQSWWKELREKGHGDLKDEPWWPKMQTIQDLIDSCTIIIWISSALHAAVNFGQY 723

Query: 301 SYGGFFPNRPTISTRLLPDEGTPDYEELELHPEKAFLKTISSQRKESISVVSIIAILSMH 360
            YGGF PNRP+ S R LP++GT DY+ELE +PEKAFLKTI+SQ +  +  VS+I ILS H
Sbjct: 724 PYGGFSPNRPSTSRRFLPEQGTQDYKELESNPEKAFLKTITSQLQALVG-VSVIEILSRH 783

Query: 361 FSDEVYLGERSNPEWTWDKEALEAFDKFRGRLKEIEERILKRNKDPKLKNRVGPVNVPYT 420
            SDEVYLG+RSNPEWT DKEALEAFDKF  RL EIE  I KRNKDP LKNRVGPV +PYT
Sbjct: 784 SSDEVYLGQRSNPEWTLDKEALEAFDKFGKRLSEIEANIGKRNKDPNLKNRVGPVEMPYT 843

Query: 421 LLFPTSTNGLTGKGIPNSISI 442
           LLFPTS+ GLTG+GIPNSISI
Sbjct: 844 LLFPTSSEGLTGRGIPNSISI 862

BLAST of BhiUN32G25 vs. NCBI nr
Match: XP_038877026.1 (probable linoleate 9S-lipoxygenase 5 [Benincasa hispida])

HSP 1 Score: 714.1 bits (1842), Expect = 7.2e-202
Identity = 341/441 (77.32%), Postives = 396/441 (89.80%), Query Frame = 0

Query: 1   MKENRLYILDHHDSIIPFLRRINSLNSTKTYATRTILFLKDDGTLKPLAIELSLPHPDGD 60
           +++N+LYIL+HHDS+IP+LRRIN+   TKTYATRTILFLK+DGTLKPLAIELSLPHP+GD
Sbjct: 425 IEKNKLYILNHHDSLIPYLRRINT-TPTKTYATRTILFLKNDGTLKPLAIELSLPHPEGD 484

Query: 61  KFGAISRVVLAAKEGVDASIWQLAKAYVAVNDTGHHQLVSHWLNTHAVIEPFVIATNRQL 120
           KFG IS+V+L AK G+D SIWQLAKAYV VNDTG+HQL+SHWLNTHA IEPFVIATNRQL
Sbjct: 485 KFGVISKVILPAKTGIDGSIWQLAKAYVTVNDTGYHQLISHWLNTHATIEPFVIATNRQL 544

Query: 121 SVLHPIHKLLVPHFKDTMKINALARESLISANGIIERTHYPAKYSMEMSSFAYKTWVFPQ 180
           SV+HPIHKLLVPHF+DTM INALAR+SLI+A+GIIE THYPAKYSMEMSSF YK+WVFPQ
Sbjct: 545 SVVHPIHKLLVPHFRDTMNINALARQSLINADGIIETTHYPAKYSMEMSSFVYKSWVFPQ 604

Query: 181 QALPADLIERGIAIKDPSAPHGVQLLIEDYPYAVDGLEIWTAIKTWVQDYCSFYYQKNEM 240
           QALPADLI+RG+AI+D S+PHG++LLIEDYPYAVDGLEIW+AIKTWVQDYCSFYY+ ++ 
Sbjct: 605 QALPADLIKRGVAIEDSSSPHGLKLLIEDYPYAVDGLEIWSAIKTWVQDYCSFYYKNDQT 664

Query: 241 ICDDQELQSWWKELREKGHEDKKNETWWPNMQTLEELVEICTIIIWISSALHAAVNFGQY 300
           + +D ELQSWWKELREKGH DKK+E WWP MQ+L++L+E CTIIIWISSALHAAVNFGQY
Sbjct: 665 LHNDTELQSWWKELREKGHADKKDEPWWPKMQSLQDLIETCTIIIWISSALHAAVNFGQY 724

Query: 301 SYGGFFPNRPTISTRLLPDEGTPDYEELELHPEKAFLKTISSQRKESISVVSIIAILSMH 360
            YGGF PNRP+ S R LP+ GTP+Y+ELE +PEKAFLKTI+SQ +  +  VS+I ILS H
Sbjct: 725 PYGGFPPNRPSTSRRFLPENGTPNYKELESNPEKAFLKTITSQLQALVG-VSVIEILSRH 784

Query: 361 FSDEVYLGERSNPEWTWDKEALEAFDKFRGRLKEIEERILKRNKDPKLKNRVGPVNVPYT 420
            SDEVYLG+RSNPEWT DKEALEAF+KF  +L EIE  I  RNKDP+LKNRVGPV++PYT
Sbjct: 785 SSDEVYLGQRSNPEWTLDKEALEAFEKFGEKLGEIEAEIAMRNKDPELKNRVGPVDMPYT 844

Query: 421 LLFPTSTNGLTGKGIPNSISI 442
           LLFPTS+ GLTG+GIPNSISI
Sbjct: 845 LLFPTSSEGLTGRGIPNSISI 863

BLAST of BhiUN32G25 vs. NCBI nr
Match: XP_022964683.1 (probable linoleate 9S-lipoxygenase 5 [Cucurbita moschata])

HSP 1 Score: 710.3 bits (1832), Expect = 1.0e-200
Identity = 339/441 (76.87%), Postives = 396/441 (89.80%), Query Frame = 0

Query: 1   MKENRLYILDHHDSIIPFLRRINSLNSTKTYATRTILFLKDDGTLKPLAIELSLPHPDGD 60
           +++N+LYIL+HHDS+IP+LRRIN   STKTYATRT+LFLK+DGTLKPLAIELSLPHP GD
Sbjct: 424 LEKNKLYILNHHDSLIPYLRRING-TSTKTYATRTLLFLKNDGTLKPLAIELSLPHPQGD 483

Query: 61  KFGAISRVVLAAKEGVDASIWQLAKAYVAVNDTGHHQLVSHWLNTHAVIEPFVIATNRQL 120
           +FGA+SRVV+ A EGV+ SIW LAKAYVAVNDTG HQL+SHWLNTHA IEPFVIATNRQL
Sbjct: 484 QFGAVSRVVVPAVEGVEGSIWLLAKAYVAVNDTGFHQLISHWLNTHATIEPFVIATNRQL 543

Query: 121 SVLHPIHKLLVPHFKDTMKINALARESLISANGIIERTHYPAKYSMEMSSFAYKTWVFPQ 180
           SV+HPIHKLLVPHF+DTM INALAR+SLI+A+GIIE THYPAKYSMEMSSF YK+WVFP 
Sbjct: 544 SVIHPIHKLLVPHFRDTMNINALARQSLINADGIIETTHYPAKYSMEMSSFVYKSWVFPD 603

Query: 181 QALPADLIERGIAIKDPSAPHGVQLLIEDYPYAVDGLEIWTAIKTWVQDYCSFYYQKNEM 240
           QALP D+I+RG+A++DPSAPHG++LLIEDYPYAVDGLEIW+AIKTWV DYCSFYY+ +E 
Sbjct: 604 QALPVDIIKRGVAMEDPSAPHGLRLLIEDYPYAVDGLEIWSAIKTWVTDYCSFYYKNDET 663

Query: 241 ICDDQELQSWWKELREKGHEDKKNETWWPNMQTLEELVEICTIIIWISSALHAAVNFGQY 300
           + +D ELQSWWKELRE+GH DKK+E WWP MQ++++L+E CTIIIWISSALHAAVNFGQY
Sbjct: 664 LRNDTELQSWWKELRERGHGDKKDEPWWPKMQSIQDLIESCTIIIWISSALHAAVNFGQY 723

Query: 301 SYGGFFPNRPTISTRLLPDEGTPDYEELELHPEKAFLKTISSQRKESISVVSIIAILSMH 360
            YGGF PNRP+ S RLLP+EGT +Y+ELE +PEKAFL+TI+SQ +  +  VS+I ILS H
Sbjct: 724 PYGGFSPNRPSTSRRLLPEEGTREYKELESNPEKAFLRTITSQLQALVG-VSVIEILSRH 783

Query: 361 FSDEVYLGERSNPEWTWDKEALEAFDKFRGRLKEIEERILKRNKDPKLKNRVGPVNVPYT 420
            SDEVYLG+RSNPEWT DKEALEAF++F  RL EIE RI+KRNKDP+LKNRVGPV +PYT
Sbjct: 784 SSDEVYLGQRSNPEWTLDKEALEAFERFGERLGEIEGRIVKRNKDPELKNRVGPVEMPYT 843

Query: 421 LLFPTSTNGLTGKGIPNSISI 442
           LLFPTS+ GLTG+GIPNSISI
Sbjct: 844 LLFPTSSEGLTGRGIPNSISI 862

BLAST of BhiUN32G25 vs. NCBI nr
Match: KAG6583413.1 (putative linoleate 9S-lipoxygenase 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 706.4 bits (1822), Expect = 1.5e-199
Identity = 338/441 (76.64%), Postives = 394/441 (89.34%), Query Frame = 0

Query: 1   MKENRLYILDHHDSIIPFLRRINSLNSTKTYATRTILFLKDDGTLKPLAIELSLPHPDGD 60
           +++N+LYIL+HHD +IP+LRRIN   STKTYATRT+LFLK+DGTLKPLAIELSLPHP GD
Sbjct: 424 VEKNKLYILNHHDPLIPYLRRING-TSTKTYATRTLLFLKNDGTLKPLAIELSLPHPQGD 483

Query: 61  KFGAISRVVLAAKEGVDASIWQLAKAYVAVNDTGHHQLVSHWLNTHAVIEPFVIATNRQL 120
           +FGA+SRVV+ A EGV+ SIW LAKAYVAVNDTG HQL+SHWLNTHA IEPFVIATNRQL
Sbjct: 484 QFGAVSRVVVPAVEGVEGSIWLLAKAYVAVNDTGFHQLISHWLNTHATIEPFVIATNRQL 543

Query: 121 SVLHPIHKLLVPHFKDTMKINALARESLISANGIIERTHYPAKYSMEMSSFAYKTWVFPQ 180
           SV+HPIHKLLVPHF+DTM INALAR+SLI+A+GIIE THYPAKYSMEMSSF YK+WVFP 
Sbjct: 544 SVIHPIHKLLVPHFRDTMNINALARQSLINADGIIETTHYPAKYSMEMSSFVYKSWVFPD 603

Query: 181 QALPADLIERGIAIKDPSAPHGVQLLIEDYPYAVDGLEIWTAIKTWVQDYCSFYYQKNEM 240
           QALP DLI+RG+A++DPSAPHG++LLIEDYPYAVDGLEIW+AIKTWV DYCSFYY+ +E 
Sbjct: 604 QALPVDLIKRGVAMEDPSAPHGLRLLIEDYPYAVDGLEIWSAIKTWVTDYCSFYYKNDET 663

Query: 241 ICDDQELQSWWKELREKGHEDKKNETWWPNMQTLEELVEICTIIIWISSALHAAVNFGQY 300
           + +D ELQSWWKELRE+GH DKK+E WWP MQ++++L+E CTIIIWISSALHAAVNFGQY
Sbjct: 664 LRNDTELQSWWKELRERGHGDKKDEPWWPKMQSIQDLIESCTIIIWISSALHAAVNFGQY 723

Query: 301 SYGGFFPNRPTISTRLLPDEGTPDYEELELHPEKAFLKTISSQRKESISVVSIIAILSMH 360
            YGGF PNRP+ S RLLP+EGT +Y+ELE +PEKA L+TI+SQ +  +  VS+I ILS H
Sbjct: 724 PYGGFSPNRPSTSRRLLPEEGTREYKELESNPEKALLRTITSQLQALVG-VSVIEILSRH 783

Query: 361 FSDEVYLGERSNPEWTWDKEALEAFDKFRGRLKEIEERILKRNKDPKLKNRVGPVNVPYT 420
            SDEVYLG+RSNPEWT DKEALEAF++F  RL EIE RI+KRNKDP+LKNRVGPV +PYT
Sbjct: 784 SSDEVYLGQRSNPEWTLDKEALEAFERFGERLGEIEGRIVKRNKDPELKNRVGPVEMPYT 843

Query: 421 LLFPTSTNGLTGKGIPNSISI 442
           LLFPTS+ GLTG+GIPNSISI
Sbjct: 844 LLFPTSSQGLTGRGIPNSISI 862

BLAST of BhiUN32G25 vs. ExPASy TrEMBL
Match: A0A6J1D9G9 (Lipoxygenase OS=Momordica charantia OX=3673 GN=LOC111018853 PE=3 SV=1)

HSP 1 Score: 719.5 bits (1856), Expect = 8.3e-204
Identity = 347/441 (78.68%), Postives = 394/441 (89.34%), Query Frame = 0

Query: 1   MKENRLYILDHHDSIIPFLRRINSLNSTKTYATRTILFLKDDGTLKPLAIELSLPHPDGD 60
           +++N+LYILDHHDS+IP+LRRIN+   TKTYATRT+LFLK+DGTLKPLAIELSLPHP GD
Sbjct: 424 LEKNKLYILDHHDSLIPYLRRINT-TPTKTYATRTLLFLKNDGTLKPLAIELSLPHPQGD 483

Query: 61  KFGAISRVVLAAKEGVDASIWQLAKAYVAVNDTGHHQLVSHWLNTHAVIEPFVIATNRQL 120
           +FG IS+V+L AK GVD SIWQLAKAYVAVNDTG+HQL+SHWLNTHAVIEPFVIATNRQL
Sbjct: 484 QFGVISKVLLPAKGGVDGSIWQLAKAYVAVNDTGYHQLISHWLNTHAVIEPFVIATNRQL 543

Query: 121 SVLHPIHKLLVPHFKDTMKINALARESLISANGIIERTHYPAKYSMEMSSFAYKTWVFPQ 180
           SV+HPIHKLLVPHF+DTM INALAR+SLI+A+GIIE THYPAKYSMEMSSF YK WVFP+
Sbjct: 544 SVVHPIHKLLVPHFRDTMNINALARQSLINADGIIETTHYPAKYSMEMSSFVYKNWVFPE 603

Query: 181 QALPADLIERGIAIKDPSAPHGVQLLIEDYPYAVDGLEIWTAIKTWVQDYCSFYYQKNEM 240
           QALPADLI+RGIAI+DPS+PHG++LLIEDYPYAVDGLEIWTAIKTWV DYCSFYY+ NE 
Sbjct: 604 QALPADLIKRGIAIQDPSSPHGLRLLIEDYPYAVDGLEIWTAIKTWVTDYCSFYYKDNET 663

Query: 241 ICDDQELQSWWKELREKGHEDKKNETWWPNMQTLEELVEICTIIIWISSALHAAVNFGQY 300
           + +D ELQSWWKELREKGH D K+E WWP MQT+++L++ CTIIIWISSALHAAVNFGQY
Sbjct: 664 LHNDVELQSWWKELREKGHGDLKDEPWWPKMQTIQDLIDSCTIIIWISSALHAAVNFGQY 723

Query: 301 SYGGFFPNRPTISTRLLPDEGTPDYEELELHPEKAFLKTISSQRKESISVVSIIAILSMH 360
            YGGF PNRP+ S R LP++GT DY+ELE +PEKAFLKTI+SQ +  +  VS+I ILS H
Sbjct: 724 PYGGFSPNRPSTSRRFLPEQGTQDYKELESNPEKAFLKTITSQLQALVG-VSVIEILSRH 783

Query: 361 FSDEVYLGERSNPEWTWDKEALEAFDKFRGRLKEIEERILKRNKDPKLKNRVGPVNVPYT 420
            SDEVYLG+RSNPEWT DKEALEAFDKF  RL EIE  I KRNKDP LKNRVGPV +PYT
Sbjct: 784 SSDEVYLGQRSNPEWTLDKEALEAFDKFGKRLSEIEANIGKRNKDPNLKNRVGPVEMPYT 843

Query: 421 LLFPTSTNGLTGKGIPNSISI 442
           LLFPTS+ GLTG+GIPNSISI
Sbjct: 844 LLFPTSSEGLTGRGIPNSISI 862

BLAST of BhiUN32G25 vs. ExPASy TrEMBL
Match: A0A6J1HLL8 (Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111464693 PE=3 SV=1)

HSP 1 Score: 710.3 bits (1832), Expect = 5.0e-201
Identity = 339/441 (76.87%), Postives = 396/441 (89.80%), Query Frame = 0

Query: 1   MKENRLYILDHHDSIIPFLRRINSLNSTKTYATRTILFLKDDGTLKPLAIELSLPHPDGD 60
           +++N+LYIL+HHDS+IP+LRRIN   STKTYATRT+LFLK+DGTLKPLAIELSLPHP GD
Sbjct: 424 LEKNKLYILNHHDSLIPYLRRING-TSTKTYATRTLLFLKNDGTLKPLAIELSLPHPQGD 483

Query: 61  KFGAISRVVLAAKEGVDASIWQLAKAYVAVNDTGHHQLVSHWLNTHAVIEPFVIATNRQL 120
           +FGA+SRVV+ A EGV+ SIW LAKAYVAVNDTG HQL+SHWLNTHA IEPFVIATNRQL
Sbjct: 484 QFGAVSRVVVPAVEGVEGSIWLLAKAYVAVNDTGFHQLISHWLNTHATIEPFVIATNRQL 543

Query: 121 SVLHPIHKLLVPHFKDTMKINALARESLISANGIIERTHYPAKYSMEMSSFAYKTWVFPQ 180
           SV+HPIHKLLVPHF+DTM INALAR+SLI+A+GIIE THYPAKYSMEMSSF YK+WVFP 
Sbjct: 544 SVIHPIHKLLVPHFRDTMNINALARQSLINADGIIETTHYPAKYSMEMSSFVYKSWVFPD 603

Query: 181 QALPADLIERGIAIKDPSAPHGVQLLIEDYPYAVDGLEIWTAIKTWVQDYCSFYYQKNEM 240
           QALP D+I+RG+A++DPSAPHG++LLIEDYPYAVDGLEIW+AIKTWV DYCSFYY+ +E 
Sbjct: 604 QALPVDIIKRGVAMEDPSAPHGLRLLIEDYPYAVDGLEIWSAIKTWVTDYCSFYYKNDET 663

Query: 241 ICDDQELQSWWKELREKGHEDKKNETWWPNMQTLEELVEICTIIIWISSALHAAVNFGQY 300
           + +D ELQSWWKELRE+GH DKK+E WWP MQ++++L+E CTIIIWISSALHAAVNFGQY
Sbjct: 664 LRNDTELQSWWKELRERGHGDKKDEPWWPKMQSIQDLIESCTIIIWISSALHAAVNFGQY 723

Query: 301 SYGGFFPNRPTISTRLLPDEGTPDYEELELHPEKAFLKTISSQRKESISVVSIIAILSMH 360
            YGGF PNRP+ S RLLP+EGT +Y+ELE +PEKAFL+TI+SQ +  +  VS+I ILS H
Sbjct: 724 PYGGFSPNRPSTSRRLLPEEGTREYKELESNPEKAFLRTITSQLQALVG-VSVIEILSRH 783

Query: 361 FSDEVYLGERSNPEWTWDKEALEAFDKFRGRLKEIEERILKRNKDPKLKNRVGPVNVPYT 420
            SDEVYLG+RSNPEWT DKEALEAF++F  RL EIE RI+KRNKDP+LKNRVGPV +PYT
Sbjct: 784 SSDEVYLGQRSNPEWTLDKEALEAFERFGERLGEIEGRIVKRNKDPELKNRVGPVEMPYT 843

Query: 421 LLFPTSTNGLTGKGIPNSISI 442
           LLFPTS+ GLTG+GIPNSISI
Sbjct: 844 LLFPTSSEGLTGRGIPNSISI 862

BLAST of BhiUN32G25 vs. ExPASy TrEMBL
Match: A0A6J1I416 (Lipoxygenase OS=Cucurbita maxima OX=3661 GN=LOC111469465 PE=3 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 4.7e-199
Identity = 338/441 (76.64%), Postives = 393/441 (89.12%), Query Frame = 0

Query: 1   MKENRLYILDHHDSIIPFLRRINSLNSTKTYATRTILFLKDDGTLKPLAIELSLPHPDGD 60
           +++N+LYIL+HHD +IP+LRRIN   STKTYATRT+LFLK+DGTLKPLAIELSLPHP GD
Sbjct: 424 LEKNKLYILNHHDPLIPYLRRING-TSTKTYATRTLLFLKNDGTLKPLAIELSLPHPQGD 483

Query: 61  KFGAISRVVLAAKEGVDASIWQLAKAYVAVNDTGHHQLVSHWLNTHAVIEPFVIATNRQL 120
           +FGAISRVV+ A EGV+ SIW LAKAYVAVNDTG HQL+SHWLNTHA IEPFVI+TNRQL
Sbjct: 484 QFGAISRVVVPAVEGVEGSIWLLAKAYVAVNDTGFHQLISHWLNTHATIEPFVISTNRQL 543

Query: 121 SVLHPIHKLLVPHFKDTMKINALARESLISANGIIERTHYPAKYSMEMSSFAYKTWVFPQ 180
           SV+HPIHKLLVPHF+DTM INALAR+SLI+A+GIIE THYPAKYSMEMSSF YK+WVFP 
Sbjct: 544 SVIHPIHKLLVPHFRDTMNINALARQSLINADGIIETTHYPAKYSMEMSSFVYKSWVFPD 603

Query: 181 QALPADLIERGIAIKDPSAPHGVQLLIEDYPYAVDGLEIWTAIKTWVQDYCSFYYQKNEM 240
           QALP DLI+RG+A++DPSAPHG++LLIEDYPYAVDGLEIW+AIKTWV DYCSFYY+ +E 
Sbjct: 604 QALPVDLIKRGVAMEDPSAPHGLRLLIEDYPYAVDGLEIWSAIKTWVTDYCSFYYKNDET 663

Query: 241 ICDDQELQSWWKELREKGHEDKKNETWWPNMQTLEELVEICTIIIWISSALHAAVNFGQY 300
           + +D ELQSWWKELRE+GH DKK+E WWP MQ++++L+E CTIIIWISSALHAAVNFGQY
Sbjct: 664 LRNDTELQSWWKELRERGHGDKKDEPWWPKMQSIQDLIESCTIIIWISSALHAAVNFGQY 723

Query: 301 SYGGFFPNRPTISTRLLPDEGTPDYEELELHPEKAFLKTISSQRKESISVVSIIAILSMH 360
            YGGF PNRP+ S RLLP+EGT +Y+ELE +PEKAFL+TI+SQ +  +  VS+I ILS H
Sbjct: 724 PYGGFSPNRPSTSRRLLPEEGTREYKELESNPEKAFLRTITSQLQALVG-VSVIEILSRH 783

Query: 361 FSDEVYLGERSNPEWTWDKEALEAFDKFRGRLKEIEERILKRNKDPKLKNRVGPVNVPYT 420
            SDEVYLG+RSNPEWT DKEALEAF++F  RL EIE RI  RNKDP+LKNRVGPV +PYT
Sbjct: 784 SSDEVYLGQRSNPEWTLDKEALEAFERFGERLGEIEGRIGGRNKDPELKNRVGPVEMPYT 843

Query: 421 LLFPTSTNGLTGKGIPNSISI 442
           LLFPTS+ GLTG+GIPNSISI
Sbjct: 844 LLFPTSSEGLTGRGIPNSISI 862

BLAST of BhiUN32G25 vs. ExPASy TrEMBL
Match: A0A1S3BY90 (Lipoxygenase OS=Cucumis melo OX=3656 GN=LOC103494874 PE=3 SV=1)

HSP 1 Score: 700.3 bits (1806), Expect = 5.2e-198
Identity = 335/442 (75.79%), Postives = 393/442 (88.91%), Query Frame = 0

Query: 1   MKENRLYILDHHDSIIPFLRRINSLNSTKTYATRTILFLKDDGTLKPLAIELSLPHPDGD 60
           +++ +LYIL+HHDS+IP+LRRIN+   TKTYATRT+LFLK+DGTLKPLAIELSLPHP GD
Sbjct: 424 LEKKKLYILNHHDSLIPYLRRINT-TPTKTYATRTVLFLKNDGTLKPLAIELSLPHPQGD 483

Query: 61  KFGAISRVVLAAKEGVDASIWQLAKAYVAVNDTGHHQLVSHWLNTHAVIEPFVIATNRQL 120
           +FG IS+V+L +  G+DASIWQLAKAYV VNDTG+HQL+SHWLNTHA IEPFVIATNRQL
Sbjct: 484 EFGVISKVILPSNTGIDASIWQLAKAYVTVNDTGYHQLISHWLNTHATIEPFVIATNRQL 543

Query: 121 SVLHPIHKLLVPHFKDTMKINALARESLISANGIIERTHYPAKYSMEMSSFAYKT-WVFP 180
           SV+HPIHKLL+PHF+DTM INALAR+SLI+A+GIIE THYP+KYSMEMSSF YKT WVFP
Sbjct: 544 SVVHPIHKLLLPHFRDTMNINALARQSLINADGIIETTHYPSKYSMEMSSFVYKTNWVFP 603

Query: 181 QQALPADLIERGIAIKDPSAPHGVQLLIEDYPYAVDGLEIWTAIKTWVQDYCSFYYQKNE 240
           QQALPADLI+RG+AI+D ++PHG+QLLIEDYPYAVDGLEIW+AIKTWVQDYCSFYY+ ++
Sbjct: 604 QQALPADLIKRGVAIEDSNSPHGLQLLIEDYPYAVDGLEIWSAIKTWVQDYCSFYYKDDQ 663

Query: 241 MICDDQELQSWWKELREKGHEDKKNETWWPNMQTLEELVEICTIIIWISSALHAAVNFGQ 300
            + +D ELQSWWKELREKGH DKK+E WWP M +L++LVE CTIIIWISSALHAAVNFGQ
Sbjct: 664 TLHNDTELQSWWKELREKGHADKKDEPWWPRMDSLQDLVETCTIIIWISSALHAAVNFGQ 723

Query: 301 YSYGGFFPNRPTISTRLLPDEGTPDYEELELHPEKAFLKTISSQRKESISVVSIIAILSM 360
           Y YGGF PNRP+ S R LP+ GT DY+ELE +PEKAFL+TI+SQ +  +  VS+I ILS 
Sbjct: 724 YPYGGFAPNRPSTSRRFLPESGTSDYKELESNPEKAFLRTITSQLQVLVG-VSVIEILSR 783

Query: 361 HFSDEVYLGERSNPEWTWDKEALEAFDKFRGRLKEIEERILKRNKDPKLKNRVGPVNVPY 420
           H SDEVYLG+RSNPEWT DKEALEAF+KF  +L EIE +I  RNKDP+LKNRVGPV++PY
Sbjct: 784 HSSDEVYLGQRSNPEWTLDKEALEAFEKFGEKLGEIERKIAMRNKDPQLKNRVGPVDMPY 843

Query: 421 TLLFPTSTNGLTGKGIPNSISI 442
           TLLFPTS+ GLTG+GIPNSISI
Sbjct: 844 TLLFPTSSEGLTGRGIPNSISI 863

BLAST of BhiUN32G25 vs. ExPASy TrEMBL
Match: A0A5A7U3G4 (Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G00520 PE=3 SV=1)

HSP 1 Score: 700.3 bits (1806), Expect = 5.2e-198
Identity = 335/442 (75.79%), Postives = 393/442 (88.91%), Query Frame = 0

Query: 1   MKENRLYILDHHDSIIPFLRRINSLNSTKTYATRTILFLKDDGTLKPLAIELSLPHPDGD 60
           +++ +LYIL+HHDS+IP+LRRIN+   TKTYATRT+LFLK+DGTLKPLAIELSLPHP GD
Sbjct: 424 LEKKKLYILNHHDSLIPYLRRINT-TPTKTYATRTVLFLKNDGTLKPLAIELSLPHPQGD 483

Query: 61  KFGAISRVVLAAKEGVDASIWQLAKAYVAVNDTGHHQLVSHWLNTHAVIEPFVIATNRQL 120
           +FG IS+V+L +  G+DASIWQLAKAYV VNDTG+HQL+SHWLNTHA IEPFVIATNRQL
Sbjct: 484 EFGVISKVILPSNTGIDASIWQLAKAYVTVNDTGYHQLISHWLNTHATIEPFVIATNRQL 543

Query: 121 SVLHPIHKLLVPHFKDTMKINALARESLISANGIIERTHYPAKYSMEMSSFAYKT-WVFP 180
           SV+HPIHKLL+PHF+DTM INALAR+SLI+A+GIIE THYP+KYSMEMSSF YKT WVFP
Sbjct: 544 SVVHPIHKLLLPHFRDTMNINALARQSLINADGIIETTHYPSKYSMEMSSFVYKTNWVFP 603

Query: 181 QQALPADLIERGIAIKDPSAPHGVQLLIEDYPYAVDGLEIWTAIKTWVQDYCSFYYQKNE 240
           QQALPADLI+RG+AI+D ++PHG+QLLIEDYPYAVDGLEIW+AIKTWVQDYCSFYY+ ++
Sbjct: 604 QQALPADLIKRGVAIEDSNSPHGLQLLIEDYPYAVDGLEIWSAIKTWVQDYCSFYYKDDQ 663

Query: 241 MICDDQELQSWWKELREKGHEDKKNETWWPNMQTLEELVEICTIIIWISSALHAAVNFGQ 300
            + +D ELQSWWKELREKGH DKK+E WWP M +L++LVE CTIIIWISSALHAAVNFGQ
Sbjct: 664 TLHNDTELQSWWKELREKGHADKKDEPWWPRMDSLQDLVETCTIIIWISSALHAAVNFGQ 723

Query: 301 YSYGGFFPNRPTISTRLLPDEGTPDYEELELHPEKAFLKTISSQRKESISVVSIIAILSM 360
           Y YGGF PNRP+ S R LP+ GT DY+ELE +PEKAFL+TI+SQ +  +  VS+I ILS 
Sbjct: 724 YPYGGFAPNRPSTSRRFLPESGTSDYKELESNPEKAFLRTITSQLQVLVG-VSVIEILSR 783

Query: 361 HFSDEVYLGERSNPEWTWDKEALEAFDKFRGRLKEIEERILKRNKDPKLKNRVGPVNVPY 420
           H SDEVYLG+RSNPEWT DKEALEAF+KF  +L EIE +I  RNKDP+LKNRVGPV++PY
Sbjct: 784 HSSDEVYLGQRSNPEWTLDKEALEAFEKFGEKLGEIERKIAMRNKDPQLKNRVGPVDMPY 843

Query: 421 TLLFPTSTNGLTGKGIPNSISI 442
           TLLFPTS+ GLTG+GIPNSISI
Sbjct: 844 TLLFPTSSEGLTGRGIPNSISI 863

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G22400.13.0e-17463.23PLAT/LH2 domain-containing lipoxygenase family protein [more]
AT1G55020.17.7e-17064.71lipoxygenase 1 [more]
AT1G17420.11.1e-12852.37lipoxygenase 3 [more]
AT1G72520.15.8e-12549.44PLAT/LH2 domain-containing lipoxygenase family protein [more]
AT1G67560.14.2e-12350.33PLAT/LH2 domain-containing lipoxygenase family protein [more]
Match NameE-valueIdentityDescription
Q412381.1e-18468.93Linoleate 9S-lipoxygenase 6 (Fragment) OS=Solanum tuberosum OX=4113 GN=LOX1.6 PE... [more]
Q431913.2e-18468.03Probable linoleate 9S-lipoxygenase 5 OS=Solanum tuberosum OX=4113 GN=LOX1.5 PE=2... [more]
Q431907.0e-18468.71Probable linoleate 9S-lipoxygenase 4 OS=Solanum tuberosum OX=4113 GN=LOX1.4 PE=2... [more]
O225077.8e-18368.48Probable linoleate 9S-lipoxygenase 7 OS=Solanum tuberosum OX=4113 GN=LOX1.7 PE=2... [more]
O243791.3e-18268.25Linoleate 9S-lipoxygenase 2 OS=Solanum tuberosum OX=4113 GN=LOX1.2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_038877029.11.6e-257100.00probable linoleate 9S-lipoxygenase 5 [Benincasa hispida][more]
XP_022150790.11.7e-20378.68probable linoleate 9S-lipoxygenase 5 [Momordica charantia][more]
XP_038877026.17.2e-20277.32probable linoleate 9S-lipoxygenase 5 [Benincasa hispida][more]
XP_022964683.11.0e-20076.87probable linoleate 9S-lipoxygenase 5 [Cucurbita moschata][more]
KAG6583413.11.5e-19976.64putative linoleate 9S-lipoxygenase 5, partial [Cucurbita argyrosperma subsp. sor... [more]
Match NameE-valueIdentityDescription
A0A6J1D9G98.3e-20478.68Lipoxygenase OS=Momordica charantia OX=3673 GN=LOC111018853 PE=3 SV=1[more]
A0A6J1HLL85.0e-20176.87Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111464693 PE=3 SV=1[more]
A0A6J1I4164.7e-19976.64Lipoxygenase OS=Cucurbita maxima OX=3661 GN=LOC111469465 PE=3 SV=1[more]
A0A1S3BY905.2e-19875.79Lipoxygenase OS=Cucumis melo OX=3656 GN=LOC103494874 PE=3 SV=1[more]
A0A5A7U3G45.2e-19875.79Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G00520 P... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013819Lipoxygenase, C-terminalPRINTSPR00087LIPOXYGENASEcoord: 96..113
score: 72.78
coord: 78..95
score: 55.95
coord: 116..136
score: 68.64
IPR013819Lipoxygenase, C-terminalPFAMPF00305Lipoxygenasecoord: 1..424
e-value: 3.7E-213
score: 709.8
IPR013819Lipoxygenase, C-terminalPROSITEPS51393LIPOXYGENASE_3coord: 1..441
score: 203.75531
IPR001246Lipoxygenase, plantPRINTSPR00468PLTLPOXGNASEcoord: 31..55
score: 81.87
coord: 354..368
score: 68.37
NoneNo IPR availableGENE3D1.20.245.10coord: 93..439
e-value: 4.5E-146
score: 488.4
NoneNo IPR availableGENE3D3.10.450.60coord: 1..92
e-value: 1.5E-38
score: 133.8
NoneNo IPR availablePANTHERPTHR11771:SF126LINOLEATE 9S-LIPOXYGENASE 1coord: 1..441
IPR000907LipoxygenasePANTHERPTHR11771LIPOXYGENASEcoord: 1..441
IPR020834Lipoxygenase, conserved sitePROSITEPS00081LIPOXYGENASE_2coord: 123..133
IPR020833Lipoxygenase, iron binding sitePROSITEPS00711LIPOXYGENASE_1coord: 96..110
IPR036226Lipoxigenase, C-terminal domain superfamilySUPERFAMILY48484Lipoxigenasecoord: 2..441

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
BhiUN32M25BhiUN32M25mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0034440 lipid oxidation
biological_process GO:0031408 oxylipin biosynthetic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
molecular_function GO:0016491 oxidoreductase activity