Bhi12G002247 (gene) Wax gourd (B227) v1

Overview
NameBhi12G002247
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationchr12: 79500369 .. 79503231 (+)
RNA-Seq ExpressionBhi12G002247
SyntenyBhi12G002247
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTGACTTTGATTTTCTTGCTTTTTAAAGATTCAACTCTCTCTCAAGAACCCGGAGAGCTTAATGTTGCCTTCAATCTACCCAACTACCTTTCTTTCCAATTCCACCGTCTTCAATGGAGGATATCCACCAAAACCTTCTTCCTCCACCACAACCGCCGCCACCGTCGAACACCGTCGTGGACTTTCCGCCATACTTCCGATGCCCTATTTCCATGGAGCTCATGCAAGATCCAGTTACGATTTCCACTGGAGTTTCCTTTGAAAGAGCTAATATTGAGAAGTGGTTCTTCACTTACAATAAGAAAACTTGCCCTGCAACCATGCAAACCATTGTCAACTTTGATATCACACCAAATTATACGCTCAAAAGGCTCATACTTTCTTGGAAAACGAAAGAGTCTTCCTCCTCCTCCTCCTCGTCGCCATCGTCGTCGATGAAGTACGATGATGTTGTGGCTGATCTCTTGAAGAACATCGAGTCGACGCCGTTCAAGGCGAGTTCTTTGAAGAAACTACGGTTGCTCATGGCAATGGATGATGTAGCTGCGGTCGGAGCTAAACCCGTCTTTGTTCGAGCGAATGGGTTTGACGTTCTTGTCGGAATCATCACGCAAGTCGCCATTTGTGAGAGTGCCGATTTCGCTAACTTTGAAGCTTGCGAGGAGGCTTTAGGGGTATTGTTGCAAACACTAAACTCAAAAGTTTAATTTTTTTTCTTAATGTCTAACAGTAAGGGTGCTTATTCAAACATCCATCCTCTATAACCTTTTGGTCCAAAATATATGCTTTAACTGGTGGACATATAATCTAATTTGGCCAAAGATTTAAGTTTTATTTATATCTGAATAAAAGGGCTATGTTTGATAGTTTGAACAAGATGCAATAAGTAACTACTAACATTAAGTTAAAAGAGAAACCGGCATGAATGAAACTTAAAATTAGGGGTGTTCAAAAAATACAACGATTTGAAAAAATTGATTAACCCAACCCAAACAACCCGTACGATTTGGGTTGGGTTATCAACTCATTTGGGTTGGATTTGATTCAATTAAATAAAAATTTTATGGGTTCACCTAATATAATCTAAATCAATCCGAACTAACCCGAATTTATTATTAATTTAAGAATTTTTTTTTTACTTATCACACAATTATTTATACATATATTGATTTTAATTGTATTTAATTTCAATATTTTTTTAATAATTTATTTTTCAACAACTCTTAAAATAGTTTTTGCACTTTAGAAAGAAAATTTTCACTATATAAATTGAAATTGGGTAGTTAATCTTAATTCAATATATGAAAATAATTAAATTAGATTTTTACATATTTATATTTTGCTTCTTTTTAGAACAAAATTTTAAATAAATGACCCAATTAACTCGAACCAACCCAACCCAAATATTTCATAGTTGGGTTAAGTTGGGTTGGGTTCATTTTTGAATGAGGGTTATTTGGGATGAAAAAATTTACAACCCAAACAATTGGGTTGGGTCTAAAAAGTCTTTCAACCTAATTTAACTCAATCCAAGAATGTTATCACCCCTACTTGAAATACCATGAAAGACTATCGTTAAATTTGTTACAATGCTTTAAATCTTTTATATGGTGTTCGAATGACCTAAGTTAAATTCGTATTTGGCATTATTTAATTATTTGTGACTATTTACGATTATGATTCTAAGGTTTAGAGAAGTGCAATATATAAATTGTCCAACCCTAGAATTGTCATTTGTTCTATAATTTATAACATTCTCTCTAATAAAATTATCATCACTTTATCCTGTAGACATAGTTATACATTGTATGTTGATTCTTTATTCTTTTTACGTTTTTTGTATTCCTTAATTATTGATTTCGTAACAAAACTCAAAAGATTAAGTTGATGGGATGGATTACGATATGCTCAATCTTTTTTTAATATTCTTAACAGGTATTATCTCAATTCCCATTTTCCAAAACTGAAAAGCCATTCGAGTTGCTATCAAGACCAGAAGCCATCAAATCAATGGCGATTGTTTTACAAAGAGGAAGCATGGAAGGAAGGTTTTATGCCATGGAAATGCTCCAACAAATATCCAAAAAGGAATACGATTGGAACTCTTTAACAAAACATCATATCATCGACTTGTTCAAATCAATATTGGAACTTGCATCGGATGAGATAATCAAGAAAGCAAGTACAATTAAGACGACAAATTTCTCAATAATTACACAATGGTACGATATCGTCCACATGCATATTTTCATTTTATATAGCTTCACTTTTAATTACTAATTAATCTTTCGTTTTCATAGTCGATACGGAACATTGTTCTCGTCTGCCCTAGAGCTTTTGATTGAAATCATGGAATCCTCGAAAAAAAGTCGTCTTCTATCGATCGAGGCCGGTGCTGTTTGCGTCATGATTGATCTTCTTCCTGACTCAAACCGATCGAAGTGCGAGAAAATATTACACATACTCAAACTTCTAAGTGAGTGTGCAGAGGGAAGGTCAGCCATGGGGGAGCATATTATGGGGATAGCAACAGTGACAAAGAAAATGAGTATATCAAACACAGCTACAAAAATTGGGATGAAAATCTTGTGGTTGATTTGCAATTATCATCCTAGTGAGAGGGTTTTAGAAGAAATGATGGGTTGTGGGACAGTGAAGAAGCTTTTAGGGATCTTGAATGGTGATGGAAGATCTTCAACCAAGGAGAAGGCCATGAAGATCATGAAGCTTCATGGGAGTTTTTGGAGGAGGTTTCCTTGTTTTCCTTATGAGTTTAGGGATTATTTGAAATTGATAAATCAATAGAATATACGTTGAAGTGAATGAGATCAATTGGATTTTGGAGTTTTTCTCACGGTTAAA

mRNA sequence

TTTGACTTTGATTTTCTTGCTTTTTAAAGATTCAACTCTCTCTCAAGAACCCGGAGAGCTTAATGTTGCCTTCAATCTACCCAACTACCTTTCTTTCCAATTCCACCGTCTTCAATGGAGGATATCCACCAAAACCTTCTTCCTCCACCACAACCGCCGCCACCGTCGAACACCGTCGTGGACTTTCCGCCATACTTCCGATGCCCTATTTCCATGGAGCTCATGCAAGATCCAGTTACGATTTCCACTGGAGTTTCCTTTGAAAGAGCTAATATTGAGAAGTGGTTCTTCACTTACAATAAGAAAACTTGCCCTGCAACCATGCAAACCATTGTCAACTTTGATATCACACCAAATTATACGCTCAAAAGGCTCATACTTTCTTGGAAAACGAAAGAGTCTTCCTCCTCCTCCTCCTCGTCGCCATCGTCGTCGATGAAGTACGATGATGTTGTGGCTGATCTCTTGAAGAACATCGAGTCGACGCCGTTCAAGGCGAGTTCTTTGAAGAAACTACGGTTGCTCATGGCAATGGATGATGTAGCTGCGGTCGGAGCTAAACCCGTCTTTGTTCGAGCGAATGGGTTTGACGTTCTTGTCGGAATCATCACGCAAGTCGCCATTTGTGAGAGTGCCGATTTCGCTAACTTTGAAGCTTGCGAGGAGGCTTTAGGGGTATTATCTCAATTCCCATTTTCCAAAACTGAAAAGCCATTCGAGTTGCTATCAAGACCAGAAGCCATCAAATCAATGGCGATTGTTTTACAAAGAGGAAGCATGGAAGGAAGGTTTTATGCCATGGAAATGCTCCAACAAATATCCAAAAAGGAATACGATTGGAACTCTTTAACAAAACATCATATCATCGACTTGTTCAAATCAATATTGGAACTTGCATCGGATGAGATAATCAAGAAAGCAAGTACAATTAAGACGACAAATTTCTCAATAATTACACAATGTCGATACGGAACATTGTTCTCGTCTGCCCTAGAGCTTTTGATTGAAATCATGGAATCCTCGAAAAAAAGTCGTCTTCTATCGATCGAGGCCGGTGCTGTTTGCGTCATGATTGATCTTCTTCCTGACTCAAACCGATCGAAGTGCGAGAAAATATTACACATACTCAAACTTCTAAGTGAGTGTGCAGAGGGAAGGTCAGCCATGGGGGAGCATATTATGGGGATAGCAACAGTGACAAAGAAAATGAGTATATCAAACACAGCTACAAAAATTGGGATGAAAATCTTGTGGTTGATTTGCAATTATCATCCTAGTGAGAGGGTTTTAGAAGAAATGATGGGTTGTGGGACAGTGAAGAAGCTTTTAGGGATCTTGAATGGTGATGGAAGATCTTCAACCAAGGAGAAGGCCATGAAGATCATGAAGCTTCATGGGAGTTTTTGGAGGAGGTTTCCTTGTTTTCCTTATGAGTTTAGGGATTATTTGAAATTGATAAATCAATAGAATATACGTTGAAGTGAATGAGATCAATTGGATTTTGGAGTTTTTCTCACGGTTAAA

Coding sequence (CDS)

ATGGAGGATATCCACCAAAACCTTCTTCCTCCACCACAACCGCCGCCACCGTCGAACACCGTCGTGGACTTTCCGCCATACTTCCGATGCCCTATTTCCATGGAGCTCATGCAAGATCCAGTTACGATTTCCACTGGAGTTTCCTTTGAAAGAGCTAATATTGAGAAGTGGTTCTTCACTTACAATAAGAAAACTTGCCCTGCAACCATGCAAACCATTGTCAACTTTGATATCACACCAAATTATACGCTCAAAAGGCTCATACTTTCTTGGAAAACGAAAGAGTCTTCCTCCTCCTCCTCCTCGTCGCCATCGTCGTCGATGAAGTACGATGATGTTGTGGCTGATCTCTTGAAGAACATCGAGTCGACGCCGTTCAAGGCGAGTTCTTTGAAGAAACTACGGTTGCTCATGGCAATGGATGATGTAGCTGCGGTCGGAGCTAAACCCGTCTTTGTTCGAGCGAATGGGTTTGACGTTCTTGTCGGAATCATCACGCAAGTCGCCATTTGTGAGAGTGCCGATTTCGCTAACTTTGAAGCTTGCGAGGAGGCTTTAGGGGTATTATCTCAATTCCCATTTTCCAAAACTGAAAAGCCATTCGAGTTGCTATCAAGACCAGAAGCCATCAAATCAATGGCGATTGTTTTACAAAGAGGAAGCATGGAAGGAAGGTTTTATGCCATGGAAATGCTCCAACAAATATCCAAAAAGGAATACGATTGGAACTCTTTAACAAAACATCATATCATCGACTTGTTCAAATCAATATTGGAACTTGCATCGGATGAGATAATCAAGAAAGCAAGTACAATTAAGACGACAAATTTCTCAATAATTACACAATGTCGATACGGAACATTGTTCTCGTCTGCCCTAGAGCTTTTGATTGAAATCATGGAATCCTCGAAAAAAAGTCGTCTTCTATCGATCGAGGCCGGTGCTGTTTGCGTCATGATTGATCTTCTTCCTGACTCAAACCGATCGAAGTGCGAGAAAATATTACACATACTCAAACTTCTAAGTGAGTGTGCAGAGGGAAGGTCAGCCATGGGGGAGCATATTATGGGGATAGCAACAGTGACAAAGAAAATGAGTATATCAAACACAGCTACAAAAATTGGGATGAAAATCTTGTGGTTGATTTGCAATTATCATCCTAGTGAGAGGGTTTTAGAAGAAATGATGGGTTGTGGGACAGTGAAGAAGCTTTTAGGGATCTTGAATGGTGATGGAAGATCTTCAACCAAGGAGAAGGCCATGAAGATCATGAAGCTTCATGGGAGTTTTTGGAGGAGGTTTCCTTGTTTTCCTTATGAGTTTAGGGATTATTTGAAATTGATAAATCAATAG

Protein sequence

MEDIHQNLLPPPQPPPPSNTVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPNYTLKRLILSWKTKESSSSSSSSPSSSMKYDDVVADLLKNIESTPFKASSLKKLRLLMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESADFANFEACEEALGVLSQFPFSKTEKPFELLSRPEAIKSMAIVLQRGSMEGRFYAMEMLQQISKKEYDWNSLTKHHIIDLFKSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIEIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEKAMKIMKLHGSFWRRFPCFPYEFRDYLKLINQ
Homology
[Close]
BLAST of Bhi12G002247 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 185.3 bits (469), Expect = 1.1e-46
Identity = 132/429 (30.77%), Postives = 217/429 (50.58%), Query Frame = 0 

Query: 22  VDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 81
           ++ PP+F CPIS+E+M+DPV +STG++++R +IEKW F   K +CP T Q I + D+TPN
Sbjct: 10  IEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTPN 69

Query: 82  YTLKRLILSWKTKESSSS-----SSSSPSSSMKYDDVVADLLKNIESTPFKASSLKKLRL 141
           +TL+RLI SW T  +S       +   P    + + ++ D   + E+   +   LK+LR 
Sbjct: 70  HTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHEN---QVKCLKRLRQ 129

Query: 142 LMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESADFANFEACEEALGVLSQFPFSK 201
           +++ +       K     A   + L  I+       S D  N    +EAL +L     S+
Sbjct: 130 IVSEN----ATNKRCLEAAGVPEFLANIV-------SNDSENGSLTDEALNLLYHLETSE 189

Query: 202 TEKPFELLSRPE--AIKSMAIVLQRGSMEGRFYAMEMLQQISKKEYDWNSLTKHHIIDLF 261
           T     L ++ +   +KS+  ++QRG  E R YA  +L+ I +      S+T     ++F
Sbjct: 190 TVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP--EVF 249

Query: 262 KSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIEIMESSKKSRLLSIEAG 321
             ++++  D I +KA+  K     ++  C +G                  ++R  ++EAG
Sbjct: 250 TEVVQILDDRISQKAT--KAAMHILVNICPWG------------------RNRHKAVEAG 309

Query: 322 AVCVMIDLLPD----SNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTKK-MSISN 381
            + V+I+LL D    S R   E  + +L LL +CAEGR+    H   IA V KK + +S 
Sbjct: 310 VISVIIELLMDESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQ 369

Query: 382 TATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEKAMKIMKLHGS 439
           TA+   +++L  +  +  +  +L EM+  G V KL  +L       TKEKA +++KLH  
Sbjct: 370 TASDRAVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHAR 402

[Close]
BLAST of Bhi12G002247 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 172.2 bits (435), Expect = 9.7e-43
Identity = 126/449 (28.06%), Postives = 224/449 (49.89%), Query Frame = 0 

Query: 22  VDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 81
           ++ P +F CPIS+++M+DPV +STG++++R +IEKW F+  K +CP T Q I   D+TPN
Sbjct: 5   IEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTPN 64

Query: 82  YTLKRLILSWKTKESSSSSSSSPSSSMKY-DDVVADLLKNIESTPF-KASSLKKLRLLMA 141
           +TL+RLI SW T  +S      P+         +  L+K   S+   +   LK+LR +++
Sbjct: 65  HTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQIVS 124

Query: 142 MD-------DVAAVGAKPVFVRANGFDVLVGIITQVA------ICESADFAN-FEA---- 201
            +       + A V      + +N  D      + ++      +C+S    N F++    
Sbjct: 125 ENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSRSL 184

Query: 202 CEEALGVLSQFPFSKTEKPFELLSR--PEAIKSMAIVLQRGSMEGRFYAMEMLQQISKKE 261
            +EAL VL     S+T     L ++     +K++  ++QRG  E R YA  +L+++ +  
Sbjct: 185 MDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLEVA 244

Query: 262 YDWNSLTKHHIIDLFKSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIEI 321
                +      +LF  ++++  D+I  KA+  ++    ++  C +G             
Sbjct: 245 DPMQIILLER--ELFGEVIQILHDQISHKAT--RSAMQILVITCPWG------------- 304

Query: 322 MESSKKSRLLSIEAGAVCVMIDLLPD----SNRSKCEKILHILKLLSECAEGRSAMGEHI 381
                ++R  ++E G + ++I+LL D    S R   E  + +L +L +CAEGR+    H 
Sbjct: 305 -----RNRHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHG 364

Query: 382 MGIATVTKK-MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRS 441
             IA V+KK + +S   ++  +++L  +  +  +  +L+EM+  G V KL  +L     +
Sbjct: 365 AAIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGN 424

Query: 442 STKEKAMKIMKLHGSFWRRFPCFPYEFRD 444
            TKEKA +++KLH   WR  PC P    D
Sbjct: 425 KTKEKAKELLKLHARVWRESPCVPRNLYD 431

[Close]
BLAST of Bhi12G002247 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 144.1 bits (362), Expect = 2.8e-34
Identity = 124/443 (27.99%), Postives = 210/443 (47.40%), Query Frame = 0 

Query: 22  VDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTI-VNFDITP 81
           ++ P YF CPIS+E+M+DPVT  +G++++R NI KW       +CP T Q + ++ D+TP
Sbjct: 22  IEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSDLTP 81

Query: 82  NYTLKRLILSWKTKESSS-----SSSSSPSSSMKYDDVVADLLKNIESTPFKASSLKKLR 141
           N+ L+RLI  W  +  +      S+   P   +   + + +L K  +    +  +L+KL 
Sbjct: 82  NHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKLE 141

Query: 142 LLMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESAD---FANFEACEEALGVLSQF 201
           +L AMD     G     +   G  V   +I  V  C S D       +  +E+L +L   
Sbjct: 142 VL-AMD-----GNNRRLMCECG--VHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLI 201

Query: 202 PFSKTEKPFELLSRPEAIKSMAIVLQRGSMEGRFYAMEMLQQISKKEYDWNSLTKHHIID 261
                +    L+     ++S+  VL +     + Y + +L+ ++  EY  + + +    +
Sbjct: 202 GIPSNDAKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLT--EYTSSHIVERLNPE 261

Query: 262 LFKSILELASDEIIKKASTIKTTNFSIITQCR----------------YGTLFSSALELL 321
           +FK I+    D +     T  T   ++ +  R                     ++AL +L
Sbjct: 262 IFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMIL 321

Query: 322 IEIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSK--CEKILHILKLLSECAEGRSAMGEH 381
           +E   S  ++R L ++ GAV  +I+L       K   E +L +L  L  CA GR+ +  H
Sbjct: 322 LE-TSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAH 381

Query: 382 IMGIATVTKK-MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGR 437
             GIA VTK+ + +S  A    + IL  +  + P   V+EEM+  GTV+KL  +L  D  
Sbjct: 382 RGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCG 441

[Close]
BLAST of Bhi12G002247 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 138.3 bits (347), Expect = 1.5e-32
Identity = 121/438 (27.63%), Postives = 203/438 (46.35%), Query Frame = 0 

Query: 12  PQPPPPSNTVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQ 71
           P+   P +  +  P +FRCPIS+ELMQDPVT+ TG +++RA+IE W    N  TCP T  
Sbjct: 2   PRNIEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRA 61

Query: 72  TIVNFDITPNYTLKRLILSWKTKESSSSSSSSPSSSMKYDDV-VADLLKNIE-------S 131
            + +F + PN+TL+RLI  W     S+     P+     D   V  LL           S
Sbjct: 62  PLSDFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVS 121

Query: 132 TPFKASSLKKLRLLMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESADFANFEACE 191
              +A++L++LR      D   V    +    N  ++L+ I+        ++  + E   
Sbjct: 122 VRSRAAALRRLRGFARDSDKNRV----LIAAHNATEILIKIL-------FSETTSSELVS 181

Query: 192 EALGVLSQFPFSKTEKPFELLSRPEAIKSMAIVLQRGSMEGRFYAMEMLQQIS--KKEYD 251
           E+L +L   P ++  +   + S P  ++ +  +L   S+E R  A  +++ +S   K  D
Sbjct: 182 ESLALLVMLPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSAD 241

Query: 252 WNSLTKHHIIDLFKSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIEIME 311
                 +    +F+ +L+L  + I  + +           +    TLF+         + 
Sbjct: 242 LKGSISNS-ESVFEGVLDLLRNPISSRRA----------LKIGIKTLFA---------LC 301

Query: 312 SSKKSRLLSIEAGAVCVMID-LLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIAT 371
           S K +R ++I AGA  ++ID L  D +R   E+ L  ++LL    EG +A GEH + +  
Sbjct: 302 SVKSTRHIAITAGAPEILIDRLAADFDRCDTERALATVELLCRTPEGCAAFGEHALTVPL 361

Query: 372 VTKK-MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEK 431
           + K  + +S+ AT+     L  +C     ER  EE  G G V +LL ++  +     K+K
Sbjct: 362 LVKTILRVSDRATEYAAGALLALCT--AEERWREEAAGAGVVVQLLLMVQSECTERAKKK 406

Query: 432 AMKIMKLHGSFWRRFPCF 438
           A K++KL    W  +  F
Sbjct: 422 AQKLLKLLRDSWPDYNSF 406

[Close]
BLAST of Bhi12G002247 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 125.2 bits (313), Expect = 1.4e-28
Identity = 121/425 (28.47%), Postives = 196/425 (46.12%), Query Frame = 0 

Query: 17  PSNTVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNF 76
           P +  +  P +FRCPIS++LM DPVTISTG +++R +I+ W    N  TCP T   + +F
Sbjct: 7   PLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGN-TTCPVTRVALSDF 66

Query: 77  DITPNYTLKRLILSWKTKESSSSSSSSPSSSMKYDDV-VADLLKNIE-------STPFKA 136
            + PN+TL+RLI  W     S+     P+     D + V  LL           S   +A
Sbjct: 67  TLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRA 126

Query: 137 SSLKKLRLLMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESADFANFEACEEALGV 196
           +++++LR L    +   V    +    N  ++LV I+   A  E+   ++ E   E+L +
Sbjct: 127 AAIRRLRGLARDSEKNRV----LIAGHNAREILVRIL--FADIETTSLSS-ELVSESLAL 186

Query: 197 LSQFPFSKTEKPFELLSRPEAIKSMAIVLQRGSMEGRFYAMEMLQQISKKEYDWNSLTKH 256
           L     ++TE    + S P  +  M  +L   S+E R  A  +++ +         LT  
Sbjct: 187 LVLLHMTETECE-AVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMV---------LTGA 246

Query: 257 HIIDLFKSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIEIMESSKKSRL 316
             +DL   ++   SD I +    +     S     + G     AL L+       K++R 
Sbjct: 247 KSMDL--KLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLV-------KQTRH 306

Query: 317 LSIEAGAVCVMID-LLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTKK-MS 376
           L+I AGA  ++ID L  D +R   E+ L  ++LL    EG +A GEH + +  + K  + 
Sbjct: 307 LAISAGAPGILIDRLAADFDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILR 366

Query: 377 ISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEKAMKIMKL 432
           +S+ AT+     L  +C     ER  +E    G V +LL ++  D     K KA  ++KL
Sbjct: 367 VSDRATEYAAGALLALCT--AEERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKL 402

[Close]
BLAST of Bhi12G002247 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 1.6e-45
Identity = 132/429 (30.77%), Postives = 217/429 (50.58%), Query Frame = 0 

Query: 22  VDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 81
           ++ PP+F CPIS+E+M+DPV +STG++++R +IEKW F   K +CP T Q I + D+TPN
Sbjct: 10  IEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTPN 69

Query: 82  YTLKRLILSWKTKESSSS-----SSSSPSSSMKYDDVVADLLKNIESTPFKASSLKKLRL 141
           +TL+RLI SW T  +S       +   P    + + ++ D   + E+   +   LK+LR 
Sbjct: 70  HTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHEN---QVKCLKRLRQ 129

Query: 142 LMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESADFANFEACEEALGVLSQFPFSK 201
           +++ +       K     A   + L  I+       S D  N    +EAL +L     S+
Sbjct: 130 IVSEN----ATNKRCLEAAGVPEFLANIV-------SNDSENGSLTDEALNLLYHLETSE 189

Query: 202 TEKPFELLSRPE--AIKSMAIVLQRGSMEGRFYAMEMLQQISKKEYDWNSLTKHHIIDLF 261
           T     L ++ +   +KS+  ++QRG  E R YA  +L+ I +      S+T     ++F
Sbjct: 190 TVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP--EVF 249

Query: 262 KSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIEIMESSKKSRLLSIEAG 321
             ++++  D I +KA+  K     ++  C +G                  ++R  ++EAG
Sbjct: 250 TEVVQILDDRISQKAT--KAAMHILVNICPWG------------------RNRHKAVEAG 309

Query: 322 AVCVMIDLLPD----SNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTKK-MSISN 381
            + V+I+LL D    S R   E  + +L LL +CAEGR+    H   IA V KK + +S 
Sbjct: 310 VISVIIELLMDESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQ 369

Query: 382 TATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEKAMKIMKLHGS 439
           TA+   +++L  +  +  +  +L EM+  G V KL  +L       TKEKA +++KLH  
Sbjct: 370 TASDRAVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHAR 402

[Close]
BLAST of Bhi12G002247 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 1.4e-41
Identity = 126/449 (28.06%), Postives = 224/449 (49.89%), Query Frame = 0 

Query: 22  VDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 81
           ++ P +F CPIS+++M+DPV +STG++++R +IEKW F+  K +CP T Q I   D+TPN
Sbjct: 5   IEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTPN 64

Query: 82  YTLKRLILSWKTKESSSSSSSSPSSSMKY-DDVVADLLKNIESTPF-KASSLKKLRLLMA 141
           +TL+RLI SW T  +S      P+         +  L+K   S+   +   LK+LR +++
Sbjct: 65  HTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQIVS 124

Query: 142 MD-------DVAAVGAKPVFVRANGFDVLVGIITQVA------ICESADFAN-FEA---- 201
            +       + A V      + +N  D      + ++      +C+S    N F++    
Sbjct: 125 ENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSRSL 184

Query: 202 CEEALGVLSQFPFSKTEKPFELLSR--PEAIKSMAIVLQRGSMEGRFYAMEMLQQISKKE 261
            +EAL VL     S+T     L ++     +K++  ++QRG  E R YA  +L+++ +  
Sbjct: 185 MDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLEVA 244

Query: 262 YDWNSLTKHHIIDLFKSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIEI 321
                +      +LF  ++++  D+I  KA+  ++    ++  C +G             
Sbjct: 245 DPMQIILLER--ELFGEVIQILHDQISHKAT--RSAMQILVITCPWG------------- 304

Query: 322 MESSKKSRLLSIEAGAVCVMIDLLPD----SNRSKCEKILHILKLLSECAEGRSAMGEHI 381
                ++R  ++E G + ++I+LL D    S R   E  + +L +L +CAEGR+    H 
Sbjct: 305 -----RNRHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHG 364

Query: 382 MGIATVTKK-MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRS 441
             IA V+KK + +S   ++  +++L  +  +  +  +L+EM+  G V KL  +L     +
Sbjct: 365 AAIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGN 424

Query: 442 STKEKAMKIMKLHGSFWRRFPCFPYEFRD 444
            TKEKA +++KLH   WR  PC P    D
Sbjct: 425 KTKEKAKELLKLHARVWRESPCVPRNLYD 431

[Close]
BLAST of Bhi12G002247 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 4.0e-33
Identity = 124/443 (27.99%), Postives = 210/443 (47.40%), Query Frame = 0 

Query: 22  VDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTI-VNFDITP 81
           ++ P YF CPIS+E+M+DPVT  +G++++R NI KW       +CP T Q + ++ D+TP
Sbjct: 8   IEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSDLTP 67

Query: 82  NYTLKRLILSWKTKESSS-----SSSSSPSSSMKYDDVVADLLKNIESTPFKASSLKKLR 141
           N+ L+RLI  W  +  +      S+   P   +   + + +L K  +    +  +L+KL 
Sbjct: 68  NHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKLE 127

Query: 142 LLMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESAD---FANFEACEEALGVLSQF 201
           +L AMD     G     +   G  V   +I  V  C S D       +  +E+L +L   
Sbjct: 128 VL-AMD-----GNNRRLMCECG--VHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLI 187

Query: 202 PFSKTEKPFELLSRPEAIKSMAIVLQRGSMEGRFYAMEMLQQISKKEYDWNSLTKHHIID 261
                +    L+     ++S+  VL +     + Y + +L+ ++  EY  + + +    +
Sbjct: 188 GIPSNDAKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLT--EYTSSHIVERLNPE 247

Query: 262 LFKSILELASDEIIKKASTIKTTNFSIITQCR----------------YGTLFSSALELL 321
           +FK I+    D +     T  T   ++ +  R                     ++AL +L
Sbjct: 248 IFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMIL 307

Query: 322 IEIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSK--CEKILHILKLLSECAEGRSAMGEH 381
           +E   S  ++R L ++ GAV  +I+L       K   E +L +L  L  CA GR+ +  H
Sbjct: 308 LE-TSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAH 367

Query: 382 IMGIATVTKK-MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGR 437
             GIA VTK+ + +S  A    + IL  +  + P   V+EEM+  GTV+KL  +L  D  
Sbjct: 368 RGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCG 427

[Close]
BLAST of Bhi12G002247 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 2.2e-31
Identity = 121/438 (27.63%), Postives = 203/438 (46.35%), Query Frame = 0 

Query: 12  PQPPPPSNTVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQ 71
           P+   P +  +  P +FRCPIS+ELMQDPVT+ TG +++RA+IE W    N  TCP T  
Sbjct: 2   PRNIEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRA 61

Query: 72  TIVNFDITPNYTLKRLILSWKTKESSSSSSSSPSSSMKYDDV-VADLLKNIE-------S 131
            + +F + PN+TL+RLI  W     S+     P+     D   V  LL           S
Sbjct: 62  PLSDFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVS 121

Query: 132 TPFKASSLKKLRLLMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESADFANFEACE 191
              +A++L++LR      D   V    +    N  ++L+ I+        ++  + E   
Sbjct: 122 VRSRAAALRRLRGFARDSDKNRV----LIAAHNATEILIKIL-------FSETTSSELVS 181

Query: 192 EALGVLSQFPFSKTEKPFELLSRPEAIKSMAIVLQRGSMEGRFYAMEMLQQIS--KKEYD 251
           E+L +L   P ++  +   + S P  ++ +  +L   S+E R  A  +++ +S   K  D
Sbjct: 182 ESLALLVMLPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSAD 241

Query: 252 WNSLTKHHIIDLFKSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIEIME 311
                 +    +F+ +L+L  + I  + +           +    TLF+         + 
Sbjct: 242 LKGSISNS-ESVFEGVLDLLRNPISSRRA----------LKIGIKTLFA---------LC 301

Query: 312 SSKKSRLLSIEAGAVCVMID-LLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIAT 371
           S K +R ++I AGA  ++ID L  D +R   E+ L  ++LL    EG +A GEH + +  
Sbjct: 302 SVKSTRHIAITAGAPEILIDRLAADFDRCDTERALATVELLCRTPEGCAAFGEHALTVPL 361

Query: 372 VTKK-MSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEK 431
           + K  + +S+ AT+     L  +C     ER  EE  G G V +LL ++  +     K+K
Sbjct: 362 LVKTILRVSDRATEYAAGALLALCT--AEERWREEAAGAGVVVQLLLMVQSECTERAKKK 406

Query: 432 AMKIMKLHGSFWRRFPCF 438
           A K++KL    W  +  F
Sbjct: 422 AQKLLKLLRDSWPDYNSF 406

[Close]
BLAST of Bhi12G002247 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.9e-27
Identity = 121/425 (28.47%), Postives = 196/425 (46.12%), Query Frame = 0 

Query: 17  PSNTVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNF 76
           P +  +  P +FRCPIS++LM DPVTISTG +++R +I+ W    N  TCP T   + +F
Sbjct: 7   PLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGN-TTCPVTRVALSDF 66

Query: 77  DITPNYTLKRLILSWKTKESSSSSSSSPSSSMKYDDV-VADLLKNIE-------STPFKA 136
            + PN+TL+RLI  W     S+     P+     D + V  LL           S   +A
Sbjct: 67  TLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRA 126

Query: 137 SSLKKLRLLMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESADFANFEACEEALGV 196
           +++++LR L    +   V    +    N  ++LV I+   A  E+   ++ E   E+L +
Sbjct: 127 AAIRRLRGLARDSEKNRV----LIAGHNAREILVRIL--FADIETTSLSS-ELVSESLAL 186

Query: 197 LSQFPFSKTEKPFELLSRPEAIKSMAIVLQRGSMEGRFYAMEMLQQISKKEYDWNSLTKH 256
           L     ++TE    + S P  +  M  +L   S+E R  A  +++ +         LT  
Sbjct: 187 LVLLHMTETECE-AVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMV---------LTGA 246

Query: 257 HIIDLFKSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIEIMESSKKSRL 316
             +DL   ++   SD I +    +     S     + G     AL L+       K++R 
Sbjct: 247 KSMDL--KLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLV-------KQTRH 306

Query: 317 LSIEAGAVCVMID-LLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIATVTKK-MS 376
           L+I AGA  ++ID L  D +R   E+ L  ++LL    EG +A GEH + +  + K  + 
Sbjct: 307 LAISAGAPGILIDRLAADFDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILR 366

Query: 377 ISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEKAMKIMKL 432
           +S+ AT+     L  +C     ER  +E    G V +LL ++  D     K KA  ++KL
Sbjct: 367 VSDRATEYAAGALLALCT--AEERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKL 402

[Close]
BLAST of Bhi12G002247 vs. NCBI nr
Match: XP_038875803.1 (E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida])

HSP 1 Score: 884.8 bits (2285), Expect = 3.1e-253
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 0 

Query: 1   MEDIHQNLLPPPQPPPPSNTVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MEDIHQNLLPPPQPPPPSNTVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIHQNLLPPPQPPPPSNTVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKTKESSSSSSSSPSSSMKYDDVVADLLKN 120
           YNKKTCPATMQTIVNFDITPNYTLKRLILSWKTKESSSSSSSSPSSSMKYDDVVADLLKN
Sbjct: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKTKESSSSSSSSPSSSMKYDDVVADLLKN 120

Query: 121 IESTPFKASSLKKLRLLMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESADFANFE 180
           IESTPFKASSLKKLRLLMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESADFANFE
Sbjct: 121 IESTPFKASSLKKLRLLMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESADFANFE 180

Query: 181 ACEEALGVLSQFPFSKTEKPFELLSRPEAIKSMAIVLQRGSMEGRFYAMEMLQQISKKEY 240
           ACEEALGVLSQFPFSKTEKPFELLSRPEAIKSMAIVLQRGSMEGRFYAMEMLQQISKKEY
Sbjct: 181 ACEEALGVLSQFPFSKTEKPFELLSRPEAIKSMAIVLQRGSMEGRFYAMEMLQQISKKEY 240

Query: 241 DWNSLTKHHIIDLFKSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIEIM 300
           DWNSLTKHHIIDLFKSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIEIM
Sbjct: 241 DWNSLTKHHIIDLFKSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIEIM 300

Query: 301 ESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIAT 360
           ESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIAT
Sbjct: 301 ESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIAT 360

Query: 361 VTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEKA 420
           VTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEKA
Sbjct: 361 VTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEKA 420

Query: 421 MKIMKLHGSFWRRFPCFPYEFRDYLKLINQ 451
           MKIMKLHGSFWRRFPCFPYEFRDYLKLINQ
Sbjct: 421 MKIMKLHGSFWRRFPCFPYEFRDYLKLINQ 450

[Close]
BLAST of Bhi12G002247 vs. NCBI nr
Match: XP_004145072.2 (E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN46274.1 hypothetical protein Csa_004999 [Cucumis sativus])

HSP 1 Score: 807.4 bits (2084), Expect = 6.4e-230
Identity = 415/453 (91.61%), Postives = 430/453 (94.92%), Query Frame = 0 

Query: 1   MEDIHQNLLPPPQPPPPSNTVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MEDIHQN  PPP  P P  T VDFPP+FRCPISMELMQDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIHQNPFPPPHAPTP--TTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKTKESSSSSSSS--PSSSMKYDDVVADLL 120
           YNKKTCPATMQTIVNFDITPNYTLKRLILSWK K+ S SS+SS  PSSSM+Y DVVADLL
Sbjct: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLL 120

Query: 121 KNIESTPFKASSLKKLRLLMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESADFAN 180
           KNIES+PFKASSLKKLRLLMA+DDVAAVGAKPVFVR NGF+VL+GI+TQVAICES+DFAN
Sbjct: 121 KNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFAN 180

Query: 181 FEACEEALGVLSQFPFSKTEKPFELLSRPEAIKSMAIVLQRGSMEGRFYAMEMLQQISKK 240
           FEACEEALGVLSQFPFSKTEKPFELLS PEAIKSMAIVLQRGS+EGRFYAMEMLQQISKK
Sbjct: 181 FEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKK 240

Query: 241 EYDWNSLTKHHIIDLFKSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIE 300
            YDWNSLTKH IIDLFKSILELASDEIIKKAS IKTT+ SI+T CRYGTLFSSALELLIE
Sbjct: 241 GYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI 360
           IMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI
Sbjct: 301 IMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI 360

Query: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGIL-NGDGRSSTK 420
           ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGIL NGDGRSSTK
Sbjct: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTK 420

Query: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYLKLINQ 451
           EKA K+MKLHGSFWRRFPCFPYE+RDYLKLINQ
Sbjct: 421 EKAKKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451

[Close]
BLAST of Bhi12G002247 vs. NCBI nr
Match: XP_008460073.1 (PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis melo])

HSP 1 Score: 794.7 bits (2051), Expect = 4.3e-226
Identity = 406/451 (90.02%), Postives = 423/451 (93.79%), Query Frame = 0 

Query: 1   MEDIHQNLLPPPQ-PPPPSNTVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFF 60
           MEDIHQ+  PP   P PP    VDFPP+FRCPISMELMQDPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKTKESSSSSSSSPSSSMKYDDVVADLLK 120
           TYNKKTCPATMQTIVNFDITPNYTLKRLILSWK KES SS+S  PSSSM+Y DVVADLLK
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDVVADLLK 120

Query: 121 NIESTPFKASSLKKLRLLMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESADFANF 180
           NIES+PFKASSLKKLRLLMA+DDVAAVGAKPVFVR NGF++LV I+TQVAICE +DFANF
Sbjct: 121 NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANF 180

Query: 181 EACEEALGVLSQFPFSKTEKPFELLSRPEAIKSMAIVLQRGSMEGRFYAMEMLQQISKKE 240
           EACEEALGVLSQFPFSKTEKPFELLS PEAIKSMAI+LQRGS+EGRFYAMEMLQQISKK 
Sbjct: 181 EACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG 240

Query: 241 YDWNSLTKHHIIDLFKSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIEI 300
           YDWNSLTKH IIDLFKSILELASDEIIKKAS IKT + S ITQCRYGTLFSSALELLIEI
Sbjct: 241 YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLIEI 300

Query: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIA 360
           MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKIL+ILKLLSECAEGRSAMGEH MGIA
Sbjct: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMGIA 360

Query: 361 TVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEK 420
           TVTKKMSISNTATKIGMKILW IC+YHPSERVLEEMMGCGTVKKLLGILNGDG SSTKEK
Sbjct: 361 TVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEK 420

Query: 421 AMKIMKLHGSFWRRFPCFPYEFRDYLKLINQ 451
           AMK+MKLHGSFWRRFPCFPYE+RDYLKLINQ
Sbjct: 421 AMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451

[Close]
BLAST of Bhi12G002247 vs. NCBI nr
Match: XP_023549278.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 767.7 bits (1981), Expect = 5.6e-218
Identity = 398/452 (88.05%), Postives = 420/452 (92.92%), Query Frame = 0 

Query: 1   MEDIHQNLLPPPQPPPPSNTVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MEDI QN LPP QP PP   V D PPYFRCPISME+M DPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPSQPLPPPPAVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKTKES-SSSSSSSPS-SSMKYDDVVADLL 120
           YNKKTCPATMQ IVNFDITPNYTLKRLILSWK  ES SS+SSSSP   SM+YDDVVA LL
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPGPPSMEYDDVVA-LL 120

Query: 121 KNIESTPFKASSLKKLRLLMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESADFAN 180
           KNIE +PFKASSLKKLRLLMA+DDVAAVGAKP+FV+ANGF+VLVG++TQVAICES++FAN
Sbjct: 121 KNIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFAN 180

Query: 181 FEACEEALGVLSQFPFSKTEKPFELLSRPEAIKSMAIVLQRGSMEGRFYAMEMLQQISKK 240
           FEACEEALGVLSQFPFSKTEK FELLSRPEAIKSMAIVLQRGS+EGRFYAME+LQQISKK
Sbjct: 181 FEACEEALGVLSQFPFSKTEKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKK 240

Query: 241 EYDWNSLTKHHIIDLFKSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIE 300
            YD+NSLTKHH IDLFKSILELASDEIIKKAST+K T+ SI TQCRYGTLFSSALELLIE
Sbjct: 241 GYDYNSLTKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI 360
           IMESSKK RLL IEAGAVC+MIDLLPDSNRSKCEKILH+LKLLS+CAEGR AM EHIMGI
Sbjct: 301 IMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGI 360

Query: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGR-SSTK 420
           ATVTKKMS SNTATKIG+KILWLICNYHPSERVLEEMMGCGTVKKL+GILNGDGR SSTK
Sbjct: 361 ATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTK 420

Query: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYLKLIN 450
           EKAMKIMKLHGSFWRRFPCFPYEFRDY KLIN
Sbjct: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

[Close]
BLAST of Bhi12G002247 vs. NCBI nr
Match: KAG6574816.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 763.8 bits (1971), Expect = 8.1e-217
Identity = 394/452 (87.17%), Postives = 420/452 (92.92%), Query Frame = 0 

Query: 1   MEDIHQNLLPPPQPPPPSNTVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MEDI QN LPPPQP PP   V D PPYFRCPISME+M DPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKTKES-SSSSSSSP-SSSMKYDDVVADLL 120
           YNKKTCPATMQ IVNFDITPNYTLKRLI+SWK  ES SS+SSSSP   SM+YDDVVA LL
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLIVSWKMNESCSSASSSSPRPPSMEYDDVVA-LL 120

Query: 121 KNIESTPFKASSLKKLRLLMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESADFAN 180
           KNIE +PFKASSLKKLRLLMA+DDVAAVGAKP+FV+ANGF+VLVG++TQ+AICES++FAN
Sbjct: 121 KNIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFAN 180

Query: 181 FEACEEALGVLSQFPFSKTEKPFELLSRPEAIKSMAIVLQRGSMEGRFYAMEMLQQISKK 240
           FEACEEALGVLSQFPFSK EK FELL+RPEAIKSMAIVLQRGS+EGRFYAME+LQQISKK
Sbjct: 181 FEACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKK 240

Query: 241 EYDWNSLTKHHIIDLFKSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIE 300
            YD+NSL+KHH IDLFKSILELASDEIIKKAST+K T+ SI TQCRYGTLFSSALELLIE
Sbjct: 241 GYDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI 360
           IMESSKK RLL IEAGAVC+MIDLLPDSNRSKCEKILH+LKLLS+CAEGR AM EHIMGI
Sbjct: 301 IMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGI 360

Query: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGR-SSTK 420
           ATVTKKMS SNTATKIG+KILWLICNYHPSERVLEEMMGCGTVKKL+GILNGDGR SSTK
Sbjct: 361 ATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTK 420

Query: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYLKLIN 450
           EKAMKIMKLHGSFWRRFPCFPYEFRDY KLIN
Sbjct: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

[Close]
BLAST of Bhi12G002247 vs. ExPASy TrEMBL
Match: A0A0A0KBP6 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G079190 PE=4 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 3.1e-230
Identity = 415/453 (91.61%), Postives = 430/453 (94.92%), Query Frame = 0 

Query: 1   MEDIHQNLLPPPQPPPPSNTVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MEDIHQN  PPP  P P  T VDFPP+FRCPISMELMQDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIHQNPFPPPHAPTP--TTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKTKESSSSSSSS--PSSSMKYDDVVADLL 120
           YNKKTCPATMQTIVNFDITPNYTLKRLILSWK K+ S SS+SS  PSSSM+Y DVVADLL
Sbjct: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLL 120

Query: 121 KNIESTPFKASSLKKLRLLMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESADFAN 180
           KNIES+PFKASSLKKLRLLMA+DDVAAVGAKPVFVR NGF+VL+GI+TQVAICES+DFAN
Sbjct: 121 KNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFAN 180

Query: 181 FEACEEALGVLSQFPFSKTEKPFELLSRPEAIKSMAIVLQRGSMEGRFYAMEMLQQISKK 240
           FEACEEALGVLSQFPFSKTEKPFELLS PEAIKSMAIVLQRGS+EGRFYAMEMLQQISKK
Sbjct: 181 FEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKK 240

Query: 241 EYDWNSLTKHHIIDLFKSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIE 300
            YDWNSLTKH IIDLFKSILELASDEIIKKAS IKTT+ SI+T CRYGTLFSSALELLIE
Sbjct: 241 GYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI 360
           IMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI
Sbjct: 301 IMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI 360

Query: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGIL-NGDGRSSTK 420
           ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGIL NGDGRSSTK
Sbjct: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTK 420

Query: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYLKLINQ 451
           EKA K+MKLHGSFWRRFPCFPYE+RDYLKLINQ
Sbjct: 421 EKAKKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451

[Close]
BLAST of Bhi12G002247 vs. ExPASy TrEMBL
Match: A0A1S3CC54 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498994 PE=4 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 2.1e-226
Identity = 406/451 (90.02%), Postives = 423/451 (93.79%), Query Frame = 0 

Query: 1   MEDIHQNLLPPPQ-PPPPSNTVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFF 60
           MEDIHQ+  PP   P PP    VDFPP+FRCPISMELMQDPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKTKESSSSSSSSPSSSMKYDDVVADLLK 120
           TYNKKTCPATMQTIVNFDITPNYTLKRLILSWK KES SS+S  PSSSM+Y DVVADLLK
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDVVADLLK 120

Query: 121 NIESTPFKASSLKKLRLLMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESADFANF 180
           NIES+PFKASSLKKLRLLMA+DDVAAVGAKPVFVR NGF++LV I+TQVAICE +DFANF
Sbjct: 121 NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANF 180

Query: 181 EACEEALGVLSQFPFSKTEKPFELLSRPEAIKSMAIVLQRGSMEGRFYAMEMLQQISKKE 240
           EACEEALGVLSQFPFSKTEKPFELLS PEAIKSMAI+LQRGS+EGRFYAMEMLQQISKK 
Sbjct: 181 EACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG 240

Query: 241 YDWNSLTKHHIIDLFKSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIEI 300
           YDWNSLTKH IIDLFKSILELASDEIIKKAS IKT + S ITQCRYGTLFSSALELLIEI
Sbjct: 241 YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLIEI 300

Query: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIA 360
           MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKIL+ILKLLSECAEGRSAMGEH MGIA
Sbjct: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMGIA 360

Query: 361 TVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEK 420
           TVTKKMSISNTATKIGMKILW IC+YHPSERVLEEMMGCGTVKKLLGILNGDG SSTKEK
Sbjct: 361 TVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEK 420

Query: 421 AMKIMKLHGSFWRRFPCFPYEFRDYLKLINQ 451
           AMK+MKLHGSFWRRFPCFPYE+RDYLKLINQ
Sbjct: 421 AMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451

[Close]
BLAST of Bhi12G002247 vs. ExPASy TrEMBL
Match: A0A6J1H6I8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460075 PE=4 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 5.6e-216
Identity = 392/452 (86.73%), Postives = 418/452 (92.48%), Query Frame = 0 

Query: 1   MEDIHQNLLPPPQPPPPSNTVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MEDI QN LPPPQP PP   V D PPYFRCPISME+M DPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKTKES-SSSSSSSP-SSSMKYDDVVADLL 120
           YNKKTCPATMQ IVNFDITPNYTLKRLILSWK  ES SS+SSSSP   SM+YDDVVA LL
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPRPPSMEYDDVVA-LL 120

Query: 121 KNIESTPFKASSLKKLRLLMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESADFAN 180
           KNIE +PFKASSLKKLRLLMA+DDV AVGAKP+FV+ANGF+VLVG++TQ+AICES++FAN
Sbjct: 121 KNIELSPFKASSLKKLRLLMAIDDVVAVGAKPIFVQANGFEVLVGLVTQIAICESSNFAN 180

Query: 181 FEACEEALGVLSQFPFSKTEKPFELLSRPEAIKSMAIVLQRGSMEGRFYAMEMLQQISKK 240
           FEACEEALGVLSQFPFSK EK FELL+RPEAIKSMAIVLQRGS+EGRFYAME+LQQISKK
Sbjct: 181 FEACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKK 240

Query: 241 EYDWNSLTKHHIIDLFKSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIE 300
            YD+NSL+KHH IDLFKSILELASDEIIKKAST+K T+ SI T CRYGTLFSSALELLIE
Sbjct: 241 GYDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATHCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI 360
           IMESSKK RLL IEAGAVC+MIDLLPDSNRSKCEKILH+LKLLS+CAEGR AM EHIMGI
Sbjct: 301 IMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGI 360

Query: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGR-SSTK 420
           ATVTKKMS SNTATKIG+KILWLICNYHPSERVLEEM+GCGTVKKL+GILNGDGR SSTK
Sbjct: 361 ATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMIGCGTVKKLVGILNGDGRSSSTK 420

Query: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYLKLIN 450
           EKAMKIMKLHGSFWRRFPCFPYEFRDY KLIN
Sbjct: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

[Close]
BLAST of Bhi12G002247 vs. ExPASy TrEMBL
Match: A0A6J1KWQ8 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499411 PE=4 SV=1)

HSP 1 Score: 748.0 bits (1930), Expect = 2.2e-212
Identity = 389/452 (86.06%), Postives = 413/452 (91.37%), Query Frame = 0 

Query: 1   MEDIHQNLLPPPQPPPPSNTVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MEDI QN LPPPQPP       D PPYFRCPISME+M DPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQPP----AAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKTKESSS--SSSSSPSSSMKYDDVVADLL 120
           YNKKTCPATMQ IVNFDITPNYTLKRLILSWK  ES S  SSSS    S++YDDVVA LL
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSFASSSSPRPPSVEYDDVVA-LL 120

Query: 121 KNIESTPFKASSLKKLRLLMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESADFAN 180
           KNIE +PFKASSLKKLRLLMA+DDVAAV AK +FV+ANGF+VLVG++TQVAICES++FAN
Sbjct: 121 KNIELSPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFAN 180

Query: 181 FEACEEALGVLSQFPFSKTEKPFELLSRPEAIKSMAIVLQRGSMEGRFYAMEMLQQISKK 240
           FEACEEALGVLSQFPFSKTEK FELLS+ EAIKSM IVLQRGS+EGRFYAME+LQQISKK
Sbjct: 181 FEACEEALGVLSQFPFSKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKK 240

Query: 241 EYDWNSLTKHHIIDLFKSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIE 300
            YD+NSL+KHH IDLFKSILELASDEIIKKASTIKTT+ SI TQCRYGTLFSSALELLIE
Sbjct: 241 GYDYNSLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI 360
           IMESSKK RLL IEAGA+C+MIDLLPDSNRSKCEKILH+LKLLS+CAEGR AM EHIMGI
Sbjct: 301 IMESSKKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGI 360

Query: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGR-SSTK 420
           ATVTKKMS SNTATKIG+KILWLICNYHPSERVLEEMMGCGTVKKL+GILNGDGR SSTK
Sbjct: 361 ATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTK 420

Query: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYLKLIN 450
           EKAMKIMKLHGSFWRRFPCFPYEFRDY KLIN
Sbjct: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 447

[Close]
BLAST of Bhi12G002247 vs. ExPASy TrEMBL
Match: A0A5D3DLY2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00740 PE=4 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 3.9e-201
Identity = 371/451 (82.26%), Postives = 389/451 (86.25%), Query Frame = 0 

Query: 1   MEDIHQNLLPPPQ-PPPPSNTVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFF 60
           MEDIHQ+  PP   P PP    VDFPP+FRCPISMELMQDPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKTKESSSSSSSSPSSSMKYDDVVADLLK 120
           TYNKKTCPATMQTIVNFDITPNYTLKRLILSWK KES SS+S  PSSSM+Y DVVADLLK
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDVVADLLK 120

Query: 121 NIESTPFKASSLKKLRLLMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESADFANF 180
           NIES+PFKASSLKKLRLLMA+DDVAAVGAKPVFVR NGF++LV I+TQVAICE +DF NF
Sbjct: 121 NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFTNF 180

Query: 181 EACEEALGVLSQFPFSKTEKPFELLSRPEAIKSMAIVLQRGSMEGRFYAMEMLQQISKKE 240
           EACEEALGVLSQFPFSKTEKPFELLS PEAIKSMAI+LQRGS+EGRFYAMEMLQQISKK 
Sbjct: 181 EACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG 240

Query: 241 YDWNSLTKHHIIDLFKSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIEI 300
           YDWNSLTKH IIDLFKSILELASDEIIKKAS IKTT+ S ITQCRYGTLFSSALELLIEI
Sbjct: 241 YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSTITQCRYGTLFSSALELLIEI 300

Query: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIA 360
           MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKIL+ILKLLSECAEG            
Sbjct: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEG------------ 360

Query: 361 TVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEK 420
                                   +YHPSERVLEEMMGCGTVKKLLGILNGDG SSTKEK
Sbjct: 361 ------------------------SYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEK 415

Query: 421 AMKIMKLHGSFWRRFPCFPYEFRDYLKLINQ 451
           AMK+MKLHGSFWR+FPCFPYE+RDYLKLINQ
Sbjct: 421 AMKVMKLHGSFWRKFPCFPYEYRDYLKLINQ 415

The following BLAST results are available for this feature:
BLAST of Bhi12G002247 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G35930.11.1e-4630.77plant U-box 23 [more]
AT3G52450.19.7e-4328.06plant U-box 22 [more]
AT3G11840.12.8e-3427.99plant U-box 24 [more]
AT3G19380.11.5e-3227.63plant U-box 25 [more]
AT1G49780.11.4e-2828.47plant U-box 26 [more]
BLAST of Bhi12G002247 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q84TG31.6e-4530.77E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Q9SVC61.4e-4128.06E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
Q9SF154.0e-3327.99E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ... [more]
Q9LT792.2e-3127.63U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q9FXA41.9e-2728.47U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
BLAST of Bhi12G002247 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038875803.13.1e-253100.00E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida][more]
XP_004145072.26.4e-23091.61E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN46274.1 hypothetical pro... [more]
XP_008460073.14.3e-22690.02PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis melo][more]
XP_023549278.15.6e-21888.05E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo][more]
KAG6574816.18.1e-21787.17E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sorori... [more]
BLAST of Bhi12G002247 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KBP63.1e-23091.61RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G079190 PE... [more]
A0A1S3CC542.1e-22690.02RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498994 PE=4 ... [more]
A0A6J1H6I85.6e-21686.73RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460075... [more]
A0A6J1KWQ82.2e-21286.06RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499411 P... [more]
A0A5D3DLY23.9e-20182.26RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 445..450
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 24..449
NoneNo IPR availablePANTHERPTHR22849:SF23RING-TYPE E3 UBIQUITIN TRANSFERASEcoord: 24..449
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 28..71
e-value: 8.44576E-21
score: 83.0519
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 17..95
IPR003613U box domainSMARTSM00504Ubox_2coord: 27..91
e-value: 9.3E-24
score: 94.9
IPR003613U box domainPFAMPF04564U-boxcoord: 25..95
e-value: 1.4E-16
score: 60.5
IPR003613U box domainPROSITEPS51698U_BOXcoord: 23..98
score: 31.881668
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 112..430
e-value: 5.4E-10
score: 40.4
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 17..107
e-value: 5.0E-24
score: 86.1
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 179..424

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi12M002247Bhi12M002247mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity