Bhi12G002101 (gene) Wax gourd (B227) v1

Overview


Name	Bhi12G002101
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Cytochrome P450
Location	chr12: 74839621 .. 74841829 (-)
RNA-Seq Expression	Bhi12G002101
Synteny	Bhi12G002101

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAAGAGATCAACAACGAGAGGGAAATGGAAGACTCCATCTTAGCATATTCAGCGATTTCAATTCTGAGTATTGGTATAGTTTTGGGAATCATAAAGCATTTGTTGAAATCAAATTGCTACAAGAATCTCCCTCCATGTCCACCCTCAATTCCAATTATAGGTCACCTCCATCTCCTTAAACTCCCAGTTCATAGGACACTACAAACTCTCTCACAAAAGTATGGTCCAGTACTTTCTCTTCGGTTCGGCTCTCGTTTAGTCGTCGTGGTTTCGTCCATGCAGGCTGCTGAAGAATGCTTCACCAAAAACGACATCATTTTCGCAAACCGTCCGGACTTCGCCGTGAGCCGGTGCTTGAGTTACAACCACACCACCCTTGGAGCGGCTCCCTACGGTGATCACTGGCGTAAGCTTCGTCGTGTCAGCGCACTCGAAATATTGTCTTCAAATCGTCTTAACACGAATTCCGGGATTCGGAGAGACGAAGTGAGAAGAGTAATGAGGAAGATATATGAAGCTTCGAGAGATGATTTTGGCAAAGTGGAGTTCAAATCAATGGTGAAGGAATTGACATTGAACATAACAATGAGAATGGTGGCAGGGAAGAGATATTATGGTGAAGAAGCAGCTAAAAGTAGTGAAGCAAGAACATTTCAAGCTATTGTGAATGAGCTTTTTGAATTTACTTTGTCTTCATATCCAGCTGACTTCTTGCCCATTTTGAAGTATATTGATATTCAAGGTTTTATGAAGAGGGCAAAGAAGCTTATCACAAGGGTTGATGCATTTTGGCAAGGATTGATTGATGAGCATAGAAGAGGTGAAATTAGTAATGAAGAAATGGAGAATTCTATGGTTGCTCATTTCCTTAAGTTGCAAGAGTCACAGCCCGAGTATTATACAGATGATATTATCAAAGGCTTGATTTTGGTTAGTTCATCTCATCTTTCTCTTTCTCTATGTAAATTCTGTTTTTCTAGGCTATTCTTTTTCTTGGTTTTCAACTCGTTGGAATATCTTGACTCTGTCGCAAGACAAACTGCGATTAGGCAATTGAGTAGGATGCACAAACATGAGTGTAGCCTAAATTTGATGATAGAAACAATCAACAAATAAATAATCAAACAAGAAGATCACTATTGGTGTGTACTATTTTATTATCTTGAGGAGCAACTAAGTATAGAAAATAAATTACAGATAGAGCGTTTATATATAGCATCCCTCTTAAACCTTAAACAGAATAAACCCAAAATAAAAGCTCAAAACAAAAGCAATGAGATCCCATGCTTCGTCAATCAGAGCACTAAATGACATATTCAAGCATTTCAACACTAAAAAGCACCTAAGTATTTTTCTTTGAGTTCAATAACATGTAGGGTAGGAGATTTGAACCTCTGGCTTGCATCAATTCTAGACTTATACGATTCGTGAAAGAATACATATCGTCTTTTCATCTAACAAACATATTTCATCTTACAGACGATGATACTCGGTGGCTCAGACACTACTGCGGTGACAATAGAGTGGGCAATGTCAAATCTCCTAAACCATCCATCAGTATTGAAGAAGGCAAGAGCTGAATTAGAGTCTCAACTGGGTGAAGATCAATTGCTTGAAGAAACCGATCTTTCAAAATTAAGTTACCTACGATGTATAGTCCTAGAAACTCTTCGGTTGTACCCGGCTGGCCCGCTCCTACTGCCACATAAGTCTTCAGCCGACTGTACCATATTAGGATACGACGTGCCACATGACACAATCTTATTGGTAAATGCTTGGGCTATCCATAGGGACCCCACATTATGGGAAGATCCAATGAGTTTTAAACCAGAGAGGTTTGAGAATAATAATGGGGAAGGGGATGGAAGTAGTAAGTTAATGATAGCATTTGGTTTGGGAAGAAGAGCTTGTCCAGGAACTGGAATGGCACATAGAGTGCTGGGTTTGAGTCTTGGACTGTTGATTCAATGTTTTGATTGGAAGAGTATTGAAGGTAAGGAAGTAGATATGACTGAAGGAATTGGGGTTAGTATGCCTAAAGCTCAACCACTTGAAGCATTATGTAAAGCAAGAGAAATTGCAACCAAGCTGCTTTGAAACTTTTTGAAATGCAATCAAATAACAACTCTTATGCAAAGAGATCTTCCTTAAGTTTTGTGTTGTATGGGTTTGTGGCTATTCCTTTATGCTTGTTTTAATATTTTGTCA

mRNA sequence

GAAAGAGATCAACAACGAGAGGGAAATGGAAGACTCCATCTTAGCATATTCAGCGATTTCAATTCTGAGTATTGGTATAGTTTTGGGAATCATAAAGCATTTGTTGAAATCAAATTGCTACAAGAATCTCCCTCCATGTCCACCCTCAATTCCAATTATAGGTCACCTCCATCTCCTTAAACTCCCAGTTCATAGGACACTACAAACTCTCTCACAAAAGTATGGTCCAGTACTTTCTCTTCGGTTCGGCTCTCGTTTAGTCGTCGTGGTTTCGTCCATGCAGGCTGCTGAAGAATGCTTCACCAAAAACGACATCATTTTCGCAAACCGTCCGGACTTCGCCGTGAGCCGGTGCTTGAGTTACAACCACACCACCCTTGGAGCGGCTCCCTACGGTGATCACTGGCGTAAGCTTCGTCGTGTCAGCGCACTCGAAATATTGTCTTCAAATCGTCTTAACACGAATTCCGGGATTCGGAGAGACGAAGTGAGAAGAGTAATGAGGAAGATATATGAAGCTTCGAGAGATGATTTTGGCAAAGTGGAGTTCAAATCAATGGTGAAGGAATTGACATTGAACATAACAATGAGAATGGTGGCAGGGAAGAGATATTATGGTGAAGAAGCAGCTAAAAGTAGTGAAGCAAGAACATTTCAAGCTATTGTGAATGAGCTTTTTGAATTTACTTTGTCTTCATATCCAGCTGACTTCTTGCCCATTTTGAAGTATATTGATATTCAAGGTTTTATGAAGAGGGCAAAGAAGCTTATCACAAGGGTTGATGCATTTTGGCAAGGATTGATTGATGAGCATAGAAGAGGTGAAATTAGTAATGAAGAAATGGAGAATTCTATGGTTGCTCATTTCCTTAAGTTGCAAGAGTCACAGCCCGAGTATTATACAGATGATATTATCAAAGGCTTGATTTTGACGATGATACTCGGTGGCTCAGACACTACTGCGGTGACAATAGAGTGGGCAATGTCAAATCTCCTAAACCATCCATCAGTATTGAAGAAGGCAAGAGCTGAATTAGAGTCTCAACTGGGTGAAGATCAATTGCTTGAAGAAACCGATCTTTCAAAATTAAGTTACCTACGATGTATAGTCCTAGAAACTCTTCGGTTGTACCCGGCTGGCCCGCTCCTACTGCCACATAAGTCTTCAGCCGACTGTACCATATTAGGATACGACGTGCCACATGACACAATCTTATTGGTAAATGCTTGGGCTATCCATAGGGACCCCACATTATGGGAAGATCCAATGAGTTTTAAACCAGAGAGGTTTGAGAATAATAATGGGGAAGGGGATGGAAGTAGTAAGTTAATGATAGCATTTGGTTTGGGAAGAAGAGCTTGTCCAGGAACTGGAATGGCACATAGAGTGCTGGGTTTGAGTCTTGGACTGTTGATTCAATGTTTTGATTGGAAGAGTATTGAAGGTAAGGAAGTAGATATGACTGAAGGAATTGGGGTTAGTATGCCTAAAGCTCAACCACTTGAAGCATTATGTAAAGCAAGAGAAATTGCAACCAAGCTGCTTTGAAACTTTTTGAAATGCAATCAAATAACAACTCTTATGCAAAGAGATCTTCCTTAAGTTTTGTGTTGTATGGGTTTGTGGCTATTCCTTTATGCTTGTTTTAATATTTTGTCA

Coding sequence (CDS)

ATGGAAGACTCCATCTTAGCATATTCAGCGATTTCAATTCTGAGTATTGGTATAGTTTTGGGAATCATAAAGCATTTGTTGAAATCAAATTGCTACAAGAATCTCCCTCCATGTCCACCCTCAATTCCAATTATAGGTCACCTCCATCTCCTTAAACTCCCAGTTCATAGGACACTACAAACTCTCTCACAAAAGTATGGTCCAGTACTTTCTCTTCGGTTCGGCTCTCGTTTAGTCGTCGTGGTTTCGTCCATGCAGGCTGCTGAAGAATGCTTCACCAAAAACGACATCATTTTCGCAAACCGTCCGGACTTCGCCGTGAGCCGGTGCTTGAGTTACAACCACACCACCCTTGGAGCGGCTCCCTACGGTGATCACTGGCGTAAGCTTCGTCGTGTCAGCGCACTCGAAATATTGTCTTCAAATCGTCTTAACACGAATTCCGGGATTCGGAGAGACGAAGTGAGAAGAGTAATGAGGAAGATATATGAAGCTTCGAGAGATGATTTTGGCAAAGTGGAGTTCAAATCAATGGTGAAGGAATTGACATTGAACATAACAATGAGAATGGTGGCAGGGAAGAGATATTATGGTGAAGAAGCAGCTAAAAGTAGTGAAGCAAGAACATTTCAAGCTATTGTGAATGAGCTTTTTGAATTTACTTTGTCTTCATATCCAGCTGACTTCTTGCCCATTTTGAAGTATATTGATATTCAAGGTTTTATGAAGAGGGCAAAGAAGCTTATCACAAGGGTTGATGCATTTTGGCAAGGATTGATTGATGAGCATAGAAGAGGTGAAATTAGTAATGAAGAAATGGAGAATTCTATGGTTGCTCATTTCCTTAAGTTGCAAGAGTCACAGCCCGAGTATTATACAGATGATATTATCAAAGGCTTGATTTTGACGATGATACTCGGTGGCTCAGACACTACTGCGGTGACAATAGAGTGGGCAATGTCAAATCTCCTAAACCATCCATCAGTATTGAAGAAGGCAAGAGCTGAATTAGAGTCTCAACTGGGTGAAGATCAATTGCTTGAAGAAACCGATCTTTCAAAATTAAGTTACCTACGATGTATAGTCCTAGAAACTCTTCGGTTGTACCCGGCTGGCCCGCTCCTACTGCCACATAAGTCTTCAGCCGACTGTACCATATTAGGATACGACGTGCCACATGACACAATCTTATTGGTAAATGCTTGGGCTATCCATAGGGACCCCACATTATGGGAAGATCCAATGAGTTTTAAACCAGAGAGGTTTGAGAATAATAATGGGGAAGGGGATGGAAGTAGTAAGTTAATGATAGCATTTGGTTTGGGAAGAAGAGCTTGTCCAGGAACTGGAATGGCACATAGAGTGCTGGGTTTGAGTCTTGGACTGTTGATTCAATGTTTTGATTGGAAGAGTATTGAAGGTAAGGAAGTAGATATGACTGAAGGAATTGGGGTTAGTATGCCTAAAGCTCAACCACTTGAAGCATTATGTAAAGCAAGAGAAATTGCAACCAAGCTGCTTTGA

Protein sequence

MEDSILAYSAISILSIGIVLGIIKHLLKSNCYKNLPPCPPSIPIIGHLHLLKLPVHRTLQTLSQKYGPVLSLRFGSRLVVVVSSMQAAEECFTKNDIIFANRPDFAVSRCLSYNHTTLGAAPYGDHWRKLRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIYEASRDDFGKVEFKSMVKELTLNITMRMVAGKRYYGEEAAKSSEARTFQAIVNELFEFTLSSYPADFLPILKYIDIQGFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDIIKGLILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAELESQLGEDQLLEETDLSKLSYLRCIVLETLRLYPAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMSFKPERFENNNGEGDGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKEVDMTEGIGVSMPKAQPLEALCKAREIATKLL

Homology

[Close]

BLAST of Bhi12G002101 vs. TAIR 10
Match: AT4G37310.1 (cytochrome P450, family 81, subfamily H, polypeptide 1 )

HSP 1 Score: 519.2 bits (1336), Expect = 3.6e-147
Identity = 260/503 (51.69%), Postives = 366/503 (72.76%), Query Frame = 0


Query: 13  ILSIGIVLGIIK-HLLKSNCYKNLPPCPP-SIPIIGHLHLLKLP-VHRTLQTLSQKYGPV 72
           IL+  + + I+K  LL +   KNLPP P    PIIGHLHLLK P +HRTL  LS   GPV
Sbjct: 7   ILTTLVAIFIVKIVLLVTKPNKNLPPSPNICFPIIGHLHLLKKPLLHRTLSHLSHSLGPV 66

Query: 73  LSLRFGSRLVVVVSSMQAAEECF-TKNDIIFANRPDFAVSRCLSYNHTTLGAAPYGDHWR 132
            SLR GSRL V++SS  AAEECF TKNDI+ ANRP F + + ++Y++T++  APYGDHWR
Sbjct: 67  FSLRLGSRLAVIISSPTAAEECFLTKNDIVLANRPRFIMGKYVAYDYTSMVTAPYGDHWR 126

Query: 133 KLRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIYEASRDDFGKVEFKSMVKELTLNITM 192
            LRR++ALE+ S+NRLN ++ IR DEV+ +++K+++ S +   KVE + ++  LTLN+ M
Sbjct: 127 NLRRITALEVFSTNRLNASAEIRHDEVKMLLQKLHDLSVERPAKVELRQLLTGLTLNVIM 186

Query: 193 RMVAGKRYYGE-EAAKSSEARTFQAIVNELFEFTLSSYPADFLPILKYIDIQGFMKRAKK 252
           RM+ GKR++ E +  K+  +  F+ +V E+ E + +  PADFLP L++ D +G +KRAK+
Sbjct: 187 RMMTGKRFFEEDDGGKAGISLEFRELVAEILELSAADNPADFLPALRWFDYKGLVKRAKR 246

Query: 253 LITRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDIIKGLILTMILG 312
           +  R+D+  QG +DEHR  +    E +N+M+AH L  QE +P  Y+D  IKGLIL M++G
Sbjct: 247 IGERMDSLLQGFLDEHRANK-DRLEFKNTMIAHLLDSQEKEPHNYSDQTIKGLILMMVVG 306

Query: 313 GSDTTAVTIEWAMSNLLNHPSVLKKARAELESQL---GEDQLLEETDLSKLSYLRCIVLE 372
           G+DT+A+T+EWAMSNLLNHP +L+  R  +++Q+      +LL+E DL  ++YL+ +V E
Sbjct: 307 GTDTSALTVEWAMSNLLNHPQILETTRQNIDTQMETSSSRRLLKEEDLVNMNYLKNVVSE 366

Query: 373 TLRLYPAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMSFKPERFEN 432
           TLRLYP  PL++PH  S+DC I G++VP DTI+LVN WAIHRDP++W+DP SFKPERFE 
Sbjct: 367 TLRLYPVAPLMVPHVPSSDCVIGGFNVPRDTIVLVNLWAIHRDPSVWDDPTSFKPERFEG 426

Query: 433 NNGEGDGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKEVDMTEGIG 492
           ++  G  + K+M  FGLGRRACPG  +A+RV+GL LG +IQCF+W+S  G +VDMTEG G
Sbjct: 427 SDQFGHYNGKMM-PFGLGRRACPGLSLANRVVGLLLGSMIQCFEWESGSGGQVDMTEGPG 486

Query: 493 VSMPKAQPLEALCKAREIATKLL 508
           +S+PKA+PL   C+ RE+A++LL
Sbjct: 487 LSLPKAEPLVVTCRTREMASELL 507

[Close]

BLAST of Bhi12G002101 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 519.2 bits (1336), Expect = 3.6e-147
Identity = 261/509 (51.28%), Postives = 361/509 (70.92%), Query Frame = 0


Query: 1   MEDSILAYSAISILSIGIVLGIIKHLLKSNCYKNLPPCPP-SIPIIGHLHLLKLPVHRTL 60
           ME   L +S +      +VL +I  + K     NLPP P  S+P+IGHL LLK P+HRT 
Sbjct: 1   METKTLIFSILF-----VVLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTF 60

Query: 61  QTLSQKY--GPVLSLRFGSRLVVVVSSMQAAEECFTKNDIIFANRPDFAVSRCLSYNHTT 120
            +LSQ     P+ SLR G+RLV V SS   AEECFTKND++ ANRP+F +++ ++Y++TT
Sbjct: 61  LSLSQSLNNAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTT 120

Query: 121 LGAAPYGDHWRKLRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIYEASRDDFGKVEFKS 180
           + AA YGDHWR LRR+ ++EI S++RLN+   IR+DE+RR++ ++      +F KV+ KS
Sbjct: 121 MIAASYGDHWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKS 180

Query: 181 MVKELTLNITMRMVAGKRYYGEEAAKSSEARTFQAIVNELFEFTLSSYPADFLPILKYID 240
           M+ +LT N  +RMVAGKRYYG+      EA+  + ++ ++     +    D+LP+L+   
Sbjct: 181 MLSDLTFNNILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRL-- 240

Query: 241 IQGFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDII 300
           +  +  R KKL  R+D F QGL+DE R      +E  N+M+ H L LQESQP+Y+TD II
Sbjct: 241 VSDYETRVKKLAGRLDEFLQGLVDEKREA----KEKGNTMIDHLLTLQESQPDYFTDRII 300

Query: 301 KGLILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAELESQLGEDQLLEETDLSKLSY 360
           KG +L +IL G+DT+AVT+EWA+SN+LNHP VL KAR E++ ++G D+L++E+D+S L Y
Sbjct: 301 KGNMLALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPY 360

Query: 361 LRCIVLETLRLYPAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMSF 420
           L+ IV ETLRLYPA P+LLPH +S DC + GYD+P  TILL N WAIHRDP LW+DPMSF
Sbjct: 361 LQNIVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSF 420

Query: 421 KPERFENNNGEGDGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKEV 480
           KPERFE      +G ++ ++ FGLGRRACPG+G+AHR++ L+LG LIQC +W+ I G+EV
Sbjct: 421 KPERFEK-----EGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKI-GEEV 480

Query: 481 DMTEGIGVSMPKAQPLEALCKAREIATKL 507
           DM+EG GV+MPKA+PLEA+C+AR    K+
Sbjct: 481 DMSEGKGVTMPKAKPLEAMCRARPSVVKI 492

[Close]

BLAST of Bhi12G002101 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 512.7 bits (1319), Expect = 3.4e-145
Identity = 265/498 (53.21%), Postives = 354/498 (71.08%), Query Frame = 0


Query: 13  ILSIGIVLGIIKHLLKSNCYKNLPPCPP-SIPIIGHLHLLKLPVHRTLQTLSQKYG--PV 72
           +LS+  ++G IK  L      NLPP P  ++P+IGHL LLK P+HR   ++SQ  G  P+
Sbjct: 14  VLSLIFLIGRIKRKL------NLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLGDAPI 73

Query: 73  LSLRFGSRLVVVVSSMQAAEECFTKNDIIFANRPDFAVSRCLSYNHTTLGAAPYGDHWRK 132
           +SLR G+RL+ VVSS   AEECFTKND+I ANR     ++ +SY ++T+ +A Y +HWR 
Sbjct: 74  ISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEHWRN 133

Query: 133 LRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIYEASRDDFGKVEFKSMVKELTLNITMR 192
           LRR+ ALEI S++RLN+ S IRRDE+RR++ ++   S   F KVE KSM  +LT N  +R
Sbjct: 134 LRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDLTFNNIIR 193

Query: 193 MVAGKRYYGEEAAKSSEARTFQAIVNELFEFTLSSYPADFLPILKYIDIQGFMKRAKKLI 252
           M+AGK YYG+      EA+  + ++ E    +     AD++PIL +I       R KKL 
Sbjct: 194 MLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS--ETRIKKLA 253

Query: 253 TRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDIIKGLILTMILGGS 312
            R+D F QGL+DE R G+   E+ EN+MV H L LQE+QPEYY D IIKG +L++I GG+
Sbjct: 254 GRLDEFLQGLVDEKREGK---EKKENTMVDHLLCLQETQPEYYMDRIIKGTMLSLIAGGT 313

Query: 313 DTTAVTIEWAMSNLLNHPSVLKKARAELESQLGEDQLLEETDLSKLSYLRCIVLETLRLY 372
           DTTAVT+EWA+S+LLN+P VL KAR E++  +G D+LLEE+D+  L YL+ IV ETLRLY
Sbjct: 314 DTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLRLY 373

Query: 373 PAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMSFKPERFENNNGEG 432
           PA P+LLPH +S DC + GYD+P  T+LL NAWAIHRDP LW+DP SFKPERFE      
Sbjct: 374 PAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEK----- 433

Query: 433 DGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKEVDMTEGIGVSMPK 492
           +G +K ++ FGLGRRACPG+G+A R++ LSLG LIQCF+W+ I  +EVDMTEG G++MPK
Sbjct: 434 EGEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTMPK 493

Query: 493 AQPLEALCKAREIATKLL 508
           A+PLEA+C+AR+   K+L
Sbjct: 494 ARPLEAMCRARDFVGKIL 495

[Close]

BLAST of Bhi12G002101 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 506.1 bits (1302), Expect = 3.2e-143
Identity = 258/508 (50.79%), Postives = 357/508 (70.28%), Query Frame = 0


Query: 3   DSILAYSAISILSIGIVLGIIKHLLKSNCYKNLPPCPP-SIPIIGHLHLLKLPVHRTLQT 62
           + IL  S +S+ ++ I L     L +S    NLPP P  S+P+IGHLHLLK P+HRT  +
Sbjct: 4   NQILILSFLSLFTLAIFL-----LTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLS 63

Query: 63  LSQKYG--PVLSLRFGSRLVVVVSSMQAAEECFTKNDIIFANRPDFAVSRCLSYNHTTLG 122
           LS+  G  PV  LR G+RLV V+SS   AEECFTKND++ ANRP F +S+ L YN T L 
Sbjct: 64  LSKSIGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLL 123

Query: 123 AAPYGDHWRKLRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIYEASRDDFGKVEFKSMV 182
           +A YGDHWR LRR++A+EI S++RLN+   IR+DE+RR++  +   S   F +VE K+++
Sbjct: 124 SASYGDHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLL 183

Query: 183 KELTLNITMRMVAGKRYYGEEAAKSSEARTFQAIVNELFEFTLSSYPADFLPILKYIDIQ 242
             L  N T+RM+AGKRY+GE+   + +A+  + +V+E      +  P D+L IL++  + 
Sbjct: 184 TNLASNTTIRMLAGKRYFGED---NDDAKLVKNLVSEAVTSAGAGNPIDYLSILRW--VS 243

Query: 243 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDIIKG 302
            + KR K L  R D F Q L+DE R    + +E   +M+ H L LQ+ QP+YYTD IIKG
Sbjct: 244 SYEKRIKNLGNRFDTFLQKLVDEKR----AEKEKGETMIDHLLALQDIQPDYYTDVIIKG 303

Query: 303 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAELESQLGEDQLLEETDLSKLSYLR 362
           +ILT+I+ G+DT++VT+EWAMSNLLNHP +LKKAR E++ ++G D+L++E+D+  LSYL+
Sbjct: 304 IILTLIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQ 363

Query: 363 CIVLETLRLYPAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMSFKP 422
            IVLETLR+YPA PLLLPH SS DC + GYD+P  T++L NAWA+HRDP +WEDP  FKP
Sbjct: 364 SIVLETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKP 423

Query: 423 ERFENNNGEGDGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKEVDM 482
           ERFE      +G ++ +I+FG+GRRACPG G+AHR++  +LG L+QCF+W+ +    VDM
Sbjct: 424 ERFEK-----EGEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDM 483

Query: 483 TEGIGVSMPKAQPLEALCKAREIATKLL 508
           TE  G ++PKA PL A+CKAR I  KL+
Sbjct: 484 TEDKGATLPKAIPLRAMCKARSIVDKLI 492

[Close]

BLAST of Bhi12G002101 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 502.7 bits (1293), Expect = 3.5e-142
Identity = 265/512 (51.76%), Postives = 360/512 (70.31%), Query Frame = 0


Query: 1   MEDSILAYSAISI-LSIGIVLGIIKHLLKSNCYKNLPPCPP-SIPIIGHLHLLKLPVHRT 60
           ME   L ++ + I LS+  ++G IK         NLPP P  ++P+IGHL LLK P+HR 
Sbjct: 1   MEGQTLIFTFLFISLSLTFIIGRIKR------RPNLPPSPSWALPVIGHLRLLKPPLHRV 60

Query: 61  LQTLSQKYG--PVLSLRFGSRLVVVVSSMQAAEECFTKNDIIFANRPDFAVSRCLSYNHT 120
             ++S+  G  P++SLR G+RLV VVSS   AEECFTKND++ ANR +   S+ +SY  T
Sbjct: 61  FLSVSESLGDAPIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCT 120

Query: 121 TLGAAPYGDHWRKLRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIYEASRDDFGKVEFK 180
           T+  A YGDHWR LRR+ A+EI S++RLN+ S IRRDE+ R++  +   S  +F KVE K
Sbjct: 121 TVVTASYGDHWRNLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMK 180

Query: 181 SMVKELTLNITMRMVAGKRYYGEEAAKSSEARTFQAIVNELFEFTLSSYPADFLPILKYI 240
           SM   LT N  +RM+AGK YYG+ A    EA+  + ++ E      +   AD+LPIL + 
Sbjct: 181 SMFSNLTFNNIIRMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTW- 240

Query: 241 DIQGFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDI 300
            I G  KR KK+ +R+D F QGL+DE R G+   E+ +N+MV H L LQE+QPEYYTD+I
Sbjct: 241 -ITGSEKRIKKIASRLDEFLQGLVDERREGK---EKRQNTMVDHLLCLQETQPEYYTDNI 300

Query: 301 IKGLILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAELESQLGEDQLLEETDLSKLS 360
           IKG++L++IL G+DT+AVT+EW +S LLNHP +L KAR E+++++G ++L+EE+DLS L 
Sbjct: 301 IKGIMLSLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLP 360

Query: 361 YLRCIVLETLRLYPAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMS 420
           YL+ IV E+LRLYPA PLL+PH +S DC + GY +P  T+LL NAWAIHRDP +W+DP S
Sbjct: 361 YLQNIVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTS 420

Query: 421 FKPERFENNNGEGDGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKE 480
           FKPERFE      +G ++ ++ FGLGRRACPG+G+A R+  L++G LIQCF+W+ I  +E
Sbjct: 421 FKPERFEK-----EGEAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEE 480

Query: 481 VDMTE-GIGVSMPKAQPLEALCKAREIATKLL 508
           VDMTE G GV MPKA PL A+CKAR +  K+L
Sbjct: 481 VDMTEGGGGVIMPKAIPLVAMCKARPVVGKIL 496

[Close]

BLAST of Bhi12G002101 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 1.1e-153
Identity = 275/508 (54.13%), Postives = 371/508 (73.03%), Query Frame = 0


Query: 1   MEDSILAYSAISILSIGIVLGIIKHLLKSNCYKNLPPCPP-SIPIIGHLHLLKLPVHRTL 60
           ME S L Y+ ++++ + I L   K    S   +NLPP P  ++P+IGHLHL+   +HR+L
Sbjct: 1   MESSTLLYTFLAVVLLSISL---KLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSL 60

Query: 61  QTLSQKYGPVLSLRFGSRLVVVVSSMQAAEECFTKNDIIFANRPDFAVSRCLSYNHTTLG 120
             LS+KYG V SL+ G+RLV+VVSS  AAEECFTKNDI+FANRP F + + + YN+TT+ 
Sbjct: 61  YDLSKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMV 120

Query: 121 AAPYGDHWRKLRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIYEASRDDFGKVEFKSMV 180
            +PYG+HWR LRR++A+EI S+  LN    IR DEV++++  +Y++S  DFGKVE KS +
Sbjct: 121 GSPYGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKL 180

Query: 181 KELTLNITMRMVAGKRYYGEEAAKSSEARTFQAIVNELFEFTLSSYPADFLPILKYIDIQ 240
            EL+ N+TMRMVAGKRY+G++   S EA+ F+A++ E+FE   +S P DF+P L++ID +
Sbjct: 181 SELSFNVTMRMVAGKRYFGQD-VDSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFK 240

Query: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDIIKG 300
            + K+  K+   +DAF Q LI E R   I+   +  +M+ H L LQESQPEYYTD IIKG
Sbjct: 241 NYEKKVSKISQEMDAFLQRLIHESR---INKNNV--TMIDHLLSLQESQPEYYTDQIIKG 300

Query: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAELESQLGEDQLLEETDLSKLSYLR 360
           +I+ ++L G+DT+AVT+EWAMS LLNHP  L+KAR E+E+Q+G ++L+EE DL KL+YL 
Sbjct: 301 IIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLH 360

Query: 361 CIVLETLRLYPAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMSFKP 420
            I+ ET RL PA P+L+PH+SS DC + GYDVP  TILLVNAWAIHRDP  W++P  FKP
Sbjct: 361 NIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKP 420

Query: 421 ERFENNNGEGDGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKEVDM 480
           ER   + G     SKLM  FG+GRR+CPG+G+A RV+GL+LG LIQCF+WK I   ++DM
Sbjct: 421 ER---HGGVELEPSKLM-PFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDM 480

Query: 481 TEGIGVSMPKAQPLEALCKAREIATKLL 508
            EG G++MPKAQPLEALCK R I  K++
Sbjct: 481 AEGSGLTMPKAQPLEALCKPRNILHKVV 495

[Close]

BLAST of Bhi12G002101 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 533.5 bits (1373), Expect = 2.6e-150
Identity = 257/505 (50.89%), Postives = 369/505 (73.07%), Query Frame = 0


Query: 4   SILAYSAISILSIGIVLGIIKHLLKSNCYKNLPPCPPSIPIIGHLHLLKLPVHRTLQTLS 63
           S+L+YS +  L++  +  I+   +++  +KNLPP PPS+PIIG+LH LK P+HRT + LS
Sbjct: 5   SLLSYS-VFYLALFFIFNIV---IRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLS 64

Query: 64  QKYGPVLSLRFGSRLVVVVSSMQAAEECFTKNDIIFANRPDFAVSRCLSYNHTTLGAAPY 123
           +KYG V SL FGSRLVVVVSS    ++CFTKND++ ANRP F   + + YN+TTLG+  Y
Sbjct: 65  EKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSY 124

Query: 124 GDHWRKLRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIYEASRDDFGKVEFKSMVKELT 183
           G+HWR LRR++AL++LS++R+N+ SGIRRDE +R++ ++ + S  +F ++E  S + ++T
Sbjct: 125 GEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMT 184

Query: 184 LNITMRMVAGKRYYGEEAAKS--SEARTFQAIVNELFEFTLSSYPADFLPILKYIDIQGF 243
            N  MRM++GKRYYGE+   S   EA  F+ +V+EL + + ++   DF+P+L+++D +  
Sbjct: 185 FNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENL 244

Query: 244 MKRAKKLITRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDIIKGLI 303
            KR K +  + DAF +GLI+EHR    + +E  N+M+ H L LQ+SQPEYYTD IIKGL 
Sbjct: 245 EKRLKDISGKTDAFLRGLIEEHR----TKKERANTMIDHLLNLQDSQPEYYTDQIIKGLA 304

Query: 304 LTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAELESQLGEDQLLEETDLSKLSYLRCI 363
           L M+L G+D++AVT+EW+MSNLLNHP VLKK + EL++ +G+D+L++E+DL KL+YL+ +
Sbjct: 305 LAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNV 364

Query: 364 VLETLRLYPAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMSFKPER 423
           + ETLRLY   PLLLPH +S +C I GY VP DTI+L+NAWAIHRDP LW +  +FKPER
Sbjct: 365 INETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPER 424

Query: 424 FENNNGEGDGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKEVDMTE 483
           FE       G  + +IAFG+GRRACPG G+A R + ++L LLIQCFDWK I G ++D+ E
Sbjct: 425 FEKK-----GELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAE 484

Query: 484 GIGVSMPKAQPLEALCKAREIATKL 507
             G ++ K  PL+A+CK+R +  K+
Sbjct: 485 RDGFTLTKLVPLKAMCKSRPVINKV 496

[Close]

BLAST of Bhi12G002101 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 1.7e-149
Identity = 271/499 (54.31%), Postives = 360/499 (72.14%), Query Frame = 0


Query: 12  SILSIGIVLGIIKHLLK--SNCYKNLPPCPPSIPIIGHLHLLKLPVHRTLQTLSQKYGPV 71
           S+LS+  ++  IK LLK  S   KNLPP PP+IPIIG+LH LK P+HRT  TLSQ YG +
Sbjct: 6   SLLSLSFII-TIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDI 65

Query: 72  LSLRFGSRLVVVVSSMQAAEECFTKNDIIFANRPDFAVSRCLSYNHTTLGAAPYGDHWRK 131
            SL FGSRLVVVVSS   A ECFTKNDII ANRP F   + + YN+TTLG+A YGDHWR 
Sbjct: 66  FSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRN 125

Query: 132 LRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIY-EASRDDFG--KVEFKSMVKELTLNI 191
           LRR++ +++LS+NRLN+  G+RRDE  R+++K+  +   + FG  KVE +  + E+T N 
Sbjct: 126 LRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNA 185

Query: 192 TMRMVAGKRYYGEEAAKS--SEARTFQAIVNELFEFTLSSYPADFLPILKYIDIQGFMKR 251
            MRM++GKRYYG++   S   EA+ F+ I++E+     ++   DFLP+L+ +D+    KR
Sbjct: 186 MMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKR 245

Query: 252 AKKLITRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDIIKGLILTM 311
            K++  R +AF +GLI+EHRRG I ++    +M+ H LKL ESQPEYY+D +IKGLI  M
Sbjct: 246 CKRIAKRSNAFLEGLIEEHRRGNIHSD--GGTMIDHLLKLSESQPEYYSDHLIKGLIQGM 305

Query: 312 ILGGSDTTAVTIEWAMSNLLNHPSVLKKARAELESQLGEDQLLEETDLSKLSYLRCIVLE 371
           +L G+DT+AVTIEW MS LLNHP VLKKA+ EL++Q+G+++L++E DLSKL YL+ I+ E
Sbjct: 306 LLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISE 365

Query: 372 TLRLYPAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMSFKPERFEN 431
           TLRL+P  PLLLPH SS DCTI  ++VP DTI+L N W IHRDP  W D +SFKPERFE 
Sbjct: 366 TLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFEK 425

Query: 432 NNGEGDGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKEVDMTEGIG 491
                +  +K+M AFGLGRRACPG  +A R +G ++GLLIQCF+W+    +++DM EG G
Sbjct: 426 E----EEVNKVM-AFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKG 485

Query: 492 VSMPKAQPLEALCKAREIA 504
           ++MP   PL A+CKA  IA
Sbjct: 486 ITMPMKIPLRAMCKALPIA 496

[Close]

BLAST of Bhi12G002101 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 2.5e-148
Identity = 257/474 (54.22%), Postives = 340/474 (71.73%), Query Frame = 0


Query: 29  SNCYKNLPPCPPSIPIIGHLHLLKLPVHRTLQTLSQKYGPVLSLRFGSRLVVVVSSMQAA 88
           S  +KNLPP P  +PIIG+LH LK P+H T  TLSQKYG + SL FGSRLVVVVSS+  A
Sbjct: 26  SRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQIFSLWFGSRLVVVVSSLTIA 85

Query: 89  EECFTKNDIIFANRPDFAVSRCLSYNHTTLGAAPYGDHWRKLRRVSALEILSSNRLNTNS 148
           +ECFTKNDI+ ANRP F   + + YN+TT+  +PYGDHWR LRR+ ++EILSS+RLN+  
Sbjct: 86  QECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNLRRILSIEILSSHRLNSFL 145

Query: 149 GIRRDEVRRVMRKIYEASRDDFGKVEFKSMVKELTLNITMRMVAGKRYYGEEAAKS--SE 208
            IRRDE+ R+++K+ + S + F +VE + M  E+T N  MRMV+GKRYYG +   S   E
Sbjct: 146 EIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTIMRMVSGKRYYGNDCDVSDVEE 205

Query: 209 ARTFQAIVNELFEFTLSSYPADFLPILKYIDIQGFMKRAKKLITRVDAFWQGLIDEHRRG 268
           AR F+ I+ E+     ++   DFL  L++ D  G  KR KK+  R DAF QGLIDEHR G
Sbjct: 206 ARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLKKISKRTDAFLQGLIDEHRFG 265

Query: 269 EISNEEMENSMVAHFLKLQESQPEYYTDDIIKGLILTMILGGSDTTAVTIEWAMSNLLNH 328
           + ++    N+M+ H L  Q+SQPEYYTD IIKGL++ M+L G+DT++VTIEWAMSNLLNH
Sbjct: 266 KRNS----NTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLLAGTDTSSVTIEWAMSNLLNH 325

Query: 329 PSVLKKARAELESQLGEDQLLEETDLSKLSYLRCIVLETLRLYPAGPLLLPHKSSADCTI 388
           P ++KKA+ EL++ +G D+ ++E D+SKL YL+ IV ETLRL+ A PLL+PH SS D ++
Sbjct: 326 PEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETLRLHAAAPLLVPHLSSEDFSL 385

Query: 389 LGYDVPHDTILLVNAWAIHRDPTLWEDPMSFKPERFENNNGEGDGSSKLMIAFGLGRRAC 448
            GY++P +TIL+VNAW IHRDP LW DP  FKPERFE      +G    +++FGLGRRAC
Sbjct: 386 GGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEK-----EGEVNKLLSFGLGRRAC 445

Query: 449 PGTGMAHRVLGLSLGLLIQCFDWKSIEGKEVDMTEGIGVSMPKAQPLEALCKAR 501
           PG  ++ R  GL+LGLLIQCF+WK I  +++DM E  G++  K   L A+CK R
Sbjct: 446 PGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGITAGKKTSLNAMCKVR 490

[Close]

BLAST of Bhi12G002101 vs. ExPASy Swiss-Prot
Match: Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 1.1e-145
Identity = 250/504 (49.60%), Postives = 360/504 (71.43%), Query Frame = 0


Query: 5   ILAYSAISILSIGIVLGIIKHLLKSNCYKNLPPCPPSIPIIGHLHLLKLPVHRTLQTLSQ 64
           IL+Y   S+  + I   II+ L +S  +KNLPP P S+PIIG+LH LK P++RT + L++
Sbjct: 3   ILSYLCYSLFYLSIFF-IIRLLFQSRKFKNLPPGPTSLPIIGNLHHLKRPLNRTFKALTE 62

Query: 65  KYGPVLSLRFGSRLVVVVSSMQAAEECFTKNDIIFANRPDFAVSRCLSYNHTTLGAAPYG 124
           KYG V+SL FGSRLVVVVSS+   +ECFTKND++ ANRP F   + + YN+TTLG+  YG
Sbjct: 63  KYGNVISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYG 122

Query: 125 DHWRKLRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIYEASRDDFGKVEFKSMVKELTL 184
           +HWR LRR+++L++LS++R+N  + IRRDE +R+++K+ E S   F +VE      ++T 
Sbjct: 123 EHWRNLRRITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRFFDMTF 182

Query: 185 NITMRMVAGKRYYGEEA--AKSSEARTFQAIVNELFEFTLSSYPADFLPILKYIDIQGFM 244
           N  MRM++GKRYYG++   ++  EA  F+ +V+EL + + ++   DF+P+LK++D +   
Sbjct: 183 NNIMRMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLE 242

Query: 245 KRAKKLITRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDIIKGLIL 304
           KR K++  + D F  GL+ E R    S +E  N+M+ H L +QESQPEYYTD IIKGL L
Sbjct: 243 KRVKRIGEKNDVFLSGLLQEQR----SKKERTNTMIDHLLNMQESQPEYYTDTIIKGLCL 302

Query: 305 TMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAELESQLGEDQLLEETDLSKLSYLRCIV 364
            M+L G+D++AVT+EW MSN+LN+P VLKK R E+++ +G+D+L++E+DL KL+YLR ++
Sbjct: 303 AMLLAGTDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVI 362

Query: 365 LETLRLYPAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMSFKPERF 424
            ETLRLY   PLLLPH ++ +C + GY VP DTI+L+NAWAIHRDP  W +  +FKPERF
Sbjct: 363 YETLRLYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERF 422

Query: 425 ENNNGEGDGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKEVDMTEG 484
           +       G  + MIAFG+GRRACPG G+A R + ++L LL+QCFDWK I  +++DM+E 
Sbjct: 423 DKK-----GELEKMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSER 482

Query: 485 IGVSMPKAQPLEALCKAREIATKL 507
            G +M K  PL+A+CK R +  K+
Sbjct: 483 DGFTMTKLLPLKAMCKTRPVVNKV 496

[Close]

BLAST of Bhi12G002101 vs. NCBI nr
Match: XP_038907094.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 1010.7 bits (2612), Expect = 4.3e-291
Identity = 507/507 (100.00%), Postives = 507/507 (100.00%), Query Frame = 0


Query: 1   MEDSILAYSAISILSIGIVLGIIKHLLKSNCYKNLPPCPPSIPIIGHLHLLKLPVHRTLQ 60
           MEDSILAYSAISILSIGIVLGIIKHLLKSNCYKNLPPCPPSIPIIGHLHLLKLPVHRTLQ
Sbjct: 1   MEDSILAYSAISILSIGIVLGIIKHLLKSNCYKNLPPCPPSIPIIGHLHLLKLPVHRTLQ 60

Query: 61  TLSQKYGPVLSLRFGSRLVVVVSSMQAAEECFTKNDIIFANRPDFAVSRCLSYNHTTLGA 120
           TLSQKYGPVLSLRFGSRLVVVVSSMQAAEECFTKNDIIFANRPDFAVSRCLSYNHTTLGA
Sbjct: 61  TLSQKYGPVLSLRFGSRLVVVVSSMQAAEECFTKNDIIFANRPDFAVSRCLSYNHTTLGA 120

Query: 121 APYGDHWRKLRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIYEASRDDFGKVEFKSMVK 180
           APYGDHWRKLRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIYEASRDDFGKVEFKSMVK
Sbjct: 121 APYGDHWRKLRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIYEASRDDFGKVEFKSMVK 180

Query: 181 ELTLNITMRMVAGKRYYGEEAAKSSEARTFQAIVNELFEFTLSSYPADFLPILKYIDIQG 240
           ELTLNITMRMVAGKRYYGEEAAKSSEARTFQAIVNELFEFTLSSYPADFLPILKYIDIQG
Sbjct: 181 ELTLNITMRMVAGKRYYGEEAAKSSEARTFQAIVNELFEFTLSSYPADFLPILKYIDIQG 240

Query: 241 FMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDIIKGL 300
           FMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDIIKGL
Sbjct: 241 FMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDIIKGL 300

Query: 301 ILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAELESQLGEDQLLEETDLSKLSYLRC 360
           ILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAELESQLGEDQLLEETDLSKLSYLRC
Sbjct: 301 ILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAELESQLGEDQLLEETDLSKLSYLRC 360

Query: 361 IVLETLRLYPAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMSFKPE 420
           IVLETLRLYPAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMSFKPE
Sbjct: 361 IVLETLRLYPAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMSFKPE 420

Query: 421 RFENNNGEGDGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKEVDMT 480
           RFENNNGEGDGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKEVDMT
Sbjct: 421 RFENNNGEGDGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKEVDMT 480

Query: 481 EGIGVSMPKAQPLEALCKAREIATKLL 508
           EGIGVSMPKAQPLEALCKAREIATKLL
Sbjct: 481 EGIGVSMPKAQPLEALCKAREIATKLL 507

[Close]

BLAST of Bhi12G002101 vs. NCBI nr
Match: XP_008459942.1 (PREDICTED: isoflavone 3'-hydroxylase-like [Cucumis melo] >KAA0039878.1 isoflavone 3'-hydroxylase-like [Cucumis melo var. makuwa] >TYK24623.1 isoflavone 3'-hydroxylase-like [Cucumis melo var. makuwa])

HSP 1 Score: 901.7 bits (2329), Expect = 2.8e-258
Identity = 450/508 (88.58%), Postives = 477/508 (93.90%), Query Frame = 0


Query: 1   MEDSILAYSAISILSIG-IVLGIIKHLLKSNCYKNLPPCPPSIPIIGHLHLLKLPVHRTL 60
           MEDSIL YSA SILSIG +VL IIK+LLK N  KNLPP PPSIPIIGHLHLLKLPVHRTL
Sbjct: 1   MEDSILQYSAFSILSIGVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 60

Query: 61  QTLSQKYGPVLSLRFGSRLVVVVSSMQAAEECFTKNDIIFANRPDFAVSRCLSYNHTTLG 120
           QTLSQKYGPVLSLRFGSRLVVVV+S++AAEECFTKND+IFANRP+FAVS CLSYN TTLG
Sbjct: 61  QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG 120

Query: 121 AAPYGDHWRKLRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIYEASRDDFGKVEFKSMV 180
           AAPYGDHWRKLRRVS LEIL S+RLN N  IRRDEVRR ++KI+E SRD FGKVEFK +V
Sbjct: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAVKKIFEVSRDGFGKVEFKPLV 180

Query: 181 KELTLNITMRMVAGKRYYGEEAAKSSEARTFQAIVNELFEFTLSSYPADFLPILKYIDIQ 240
           KELT+NITMRMVAGKRYYGEEAAKSSEARTFQAIV+ELFEFTLSSYPADFLPILKYIDIQ
Sbjct: 181 KELTMNITMRMVAGKRYYGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 240

Query: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDIIKG 300
           GFMKRAKKLI RVDAFWQGLIDEHRRGEISNEEM+N MVAHFLKLQE+QP+YYTDDIIKG
Sbjct: 241 GFMKRAKKLIARVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 300

Query: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAELESQLGEDQLLEETDLSKLSYLR 360
           LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAE+ESQL  D+LLEETDLSKLSYLR
Sbjct: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLDGDKLLEETDLSKLSYLR 360

Query: 361 CIVLETLRLYPAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMSFKP 420
           CIVLETLRLYPAGPLLLPHKSSADC+I GY++P DTILLVNAWAIHRDPTLWEDP SF+P
Sbjct: 361 CIVLETLRLYPAGPLLLPHKSSADCSISGYNIPRDTILLVNAWAIHRDPTLWEDPTSFRP 420

Query: 421 ERFENNNGEGDGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKEVDM 480
           ERFENNNGEGD ++KLMIAFGLGRRACPGTGMAHRV+GL LG LIQCFDWKSI+GKEVDM
Sbjct: 421 ERFENNNGEGDANNKLMIAFGLGRRACPGTGMAHRVMGLMLGTLIQCFDWKSIDGKEVDM 480

Query: 481 TEGIGVSMPKAQPLEALCKAREIATKLL 508
            EG GVSMPKAQPLEALCKAREIATKLL
Sbjct: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 508

[Close]

BLAST of Bhi12G002101 vs. NCBI nr
Match: NP_001289568.1 (isoflavone 2'-hydroxylase-like [Cucumis sativus] >AIT72031.1 cytochrome P450 [Cucumis sativus])

HSP 1 Score: 893.3 bits (2307), Expect = 9.9e-256
Identity = 446/508 (87.80%), Postives = 474/508 (93.31%), Query Frame = 0


Query: 1   MEDSILAYSAISILSIG-IVLGIIKHLLKSNCYKNLPPCPPSIPIIGHLHLLKLPVHRTL 60
           MEDSIL YSA SILSI  +VL IIK+LLK N  KNLPP PPSIPIIGHLHLLKLPVHRTL
Sbjct: 1   MEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 60

Query: 61  QTLSQKYGPVLSLRFGSRLVVVVSSMQAAEECFTKNDIIFANRPDFAVSRCLSYNHTTLG 120
           QTLSQKYGPVLSLRFGSR VVVV+S++AAEECF KNDIIFANRP+FAVS CLSYN TTLG
Sbjct: 61  QTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLG 120

Query: 121 AAPYGDHWRKLRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIYEASRDDFGKVEFKSMV 180
           AAPYGDHWRKLRRVS LEIL S+RLN N  IRRDEVRR M+KIYE SRD FGKVEFK +V
Sbjct: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLV 180

Query: 181 KELTLNITMRMVAGKRYYGEEAAKSSEARTFQAIVNELFEFTLSSYPADFLPILKYIDIQ 240
           KELT+N+TMRMVAGKRY+GEEAAKSSEARTFQAIV+ELFEFTLSSYPADFLPILKYIDIQ
Sbjct: 181 KELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 240

Query: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDIIKG 300
           GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEM+N MVAHFLKLQE+QP+YYTDDIIKG
Sbjct: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 300

Query: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAELESQLGEDQLLEETDLSKLSYLR 360
           LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAE+ESQLG D+L+EETDLSKL+YLR
Sbjct: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLR 360

Query: 361 CIVLETLRLYPAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMSFKP 420
            I+LETLRLYPAGPLLLPHKSSADC I GYD+P DT+LLVNAWAIHRDP LWEDP SFKP
Sbjct: 361 YIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKP 420

Query: 421 ERFENNNGEGDGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKEVDM 480
           ERFENNNGEGDG++KLMIAFGLGRRACPGTGMA+RV+GL LG LIQCFDWKSI+GKEVDM
Sbjct: 421 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDM 480

Query: 481 TEGIGVSMPKAQPLEALCKAREIATKLL 508
            EG GVSMPKAQPLEALCKAREIATKLL
Sbjct: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 508

[Close]

BLAST of Bhi12G002101 vs. NCBI nr
Match: KGN46394.2 (hypothetical protein Csa_005702 [Cucumis sativus])

HSP 1 Score: 893.3 bits (2307), Expect = 9.9e-256
Identity = 446/508 (87.80%), Postives = 474/508 (93.31%), Query Frame = 0


Query: 1   MEDSILAYSAISILSIG-IVLGIIKHLLKSNCYKNLPPCPPSIPIIGHLHLLKLPVHRTL 60
           MEDSIL YSA SILSI  +VL IIK+LLK N  KNLPP PPSIPIIGHLHLLKLPVHRTL
Sbjct: 22  MEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 81

Query: 61  QTLSQKYGPVLSLRFGSRLVVVVSSMQAAEECFTKNDIIFANRPDFAVSRCLSYNHTTLG 120
           QTLSQKYGPVLSLRFGSR VVVV+S++AAEECF KNDIIFANRP+FAVS CLSYN TTLG
Sbjct: 82  QTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLG 141

Query: 121 AAPYGDHWRKLRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIYEASRDDFGKVEFKSMV 180
           AAPYGDHWRKLRRVS LEIL S+RLN N  IRRDEVRR M+KIYE SRD FGKVEFK +V
Sbjct: 142 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLV 201

Query: 181 KELTLNITMRMVAGKRYYGEEAAKSSEARTFQAIVNELFEFTLSSYPADFLPILKYIDIQ 240
           KELT+N+TMRMVAGKRY+GEEAAKSSEARTFQAIV+ELFEFTLSSYPADFLPILKYIDIQ
Sbjct: 202 KELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 261

Query: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDIIKG 300
           GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEM+N MVAHFLKLQE+QP+YYTDDIIKG
Sbjct: 262 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 321

Query: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAELESQLGEDQLLEETDLSKLSYLR 360
           LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAE+ESQLG D+L+EETDLSKL+YLR
Sbjct: 322 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLR 381

Query: 361 CIVLETLRLYPAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMSFKP 420
            I+LETLRLYPAGPLLLPHKSSADC I GYD+P DT+LLVNAWAIHRDP LWEDP SFKP
Sbjct: 382 YIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKP 441

Query: 421 ERFENNNGEGDGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKEVDM 480
           ERFENNNGEGDG++KLMIAFGLGRRACPGTGMA+RV+GL LG LIQCFDWKSI+GKEVDM
Sbjct: 442 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDM 501

Query: 481 TEGIGVSMPKAQPLEALCKAREIATKLL 508
            EG GVSMPKAQPLEALCKAREIATKLL
Sbjct: 502 NEGKGVSMPKAQPLEALCKAREIATKLL 529

[Close]

BLAST of Bhi12G002101 vs. NCBI nr
Match: KAG7025244.1 (Isoflavone 2'-hydroxylase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 844.3 bits (2180), Expect = 5.3e-241
Identity = 421/507 (83.04%), Postives = 462/507 (91.12%), Query Frame = 0


Query: 1   MEDSILAYSAISILSIGIVLGIIKHLLKSNCYKNLPPCPPSIPIIGHLHLLKLPVHRTLQ 60
           M+DS   Y   S+L I +V+  I + LK    KNLPP PPSIPIIGHLHLLK PVHR+L 
Sbjct: 1   MDDSTFLY---SVLCIALVVA-ISYALKQKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLL 60

Query: 61  TLSQKYGPVLSLRFGSRLVVVVSSMQAAEECFTKNDIIFANRPDFAVSRCLSYNHTTLGA 120
           TLSQKYGPVLSLRFGSRLVVVVSS+ AAEECFTKND++FANRPDFAVS+CLSYNHTTLGA
Sbjct: 61  TLSQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGA 120

Query: 121 APYGDHWRKLRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIYEASRDDFGKVEFKSMVK 180
           APYGDHWRKLRRVSALEILS+NRLNTNS IRRDEVRR++ K+ + SRDDF +VEFKSMVK
Sbjct: 121 APYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDDFARVEFKSMVK 180

Query: 181 ELTLNITMRMVAGKRYYGEEAAKSSEARTFQAIVNELFEFTLSSYPADFLPILKYIDIQG 240
           ELTLNITMRMVAGKRYYGEE  +SSEARTF+AIV+ELFEF L+ YPADFLPILKYIDIQG
Sbjct: 181 ELTLNITMRMVAGKRYYGEETDESSEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQG 240

Query: 241 FMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDIIKGL 300
           FMKRAKKLI RVDAFWQG++DEHR  +I N+EMENSMVAHFLKLQESQP+YYTDDIIKGL
Sbjct: 241 FMKRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGL 300

Query: 301 ILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAELESQLGEDQLLEETDLSKLSYLRC 360
           ILTM+LGGSDTTAVTIEWAMSNLLNHP+VLKKARAELE+QLG DQLLEE+++ KLSYLRC
Sbjct: 301 ILTMVLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLG-DQLLEESEVPKLSYLRC 360

Query: 361 IVLETLRLYPAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMSFKPE 420
           IVLETLRLYPAGPLLLPHKSSADCT+ GYDVP DTILLVNAWAIHRDPTLWEDP SF+PE
Sbjct: 361 IVLETLRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPE 420

Query: 421 RFENNNGEGDGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKEVDMT 480
           RFE   G+G+ +SKLMIAFGLGRRACPG+GMAHRVLGL+LGLLIQCFDWKSI+GKEVDMT
Sbjct: 421 RFE--GGDGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMT 480

Query: 481 EGIGVSMPKAQPLEALCKAREIATKLL 508
           EG GVSMPKA+PLEALCK R IA+KLL
Sbjct: 481 EGKGVSMPKAEPLEALCKPRPIASKLL 500

[Close]

BLAST of Bhi12G002101 vs. ExPASy TrEMBL
Match: A0A5D3DLU7 (Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G001700 PE=3 SV=1)

HSP 1 Score: 901.7 bits (2329), Expect = 1.4e-258
Identity = 450/508 (88.58%), Postives = 477/508 (93.90%), Query Frame = 0


Query: 1   MEDSILAYSAISILSIG-IVLGIIKHLLKSNCYKNLPPCPPSIPIIGHLHLLKLPVHRTL 60
           MEDSIL YSA SILSIG +VL IIK+LLK N  KNLPP PPSIPIIGHLHLLKLPVHRTL
Sbjct: 1   MEDSILQYSAFSILSIGVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 60

Query: 61  QTLSQKYGPVLSLRFGSRLVVVVSSMQAAEECFTKNDIIFANRPDFAVSRCLSYNHTTLG 120
           QTLSQKYGPVLSLRFGSRLVVVV+S++AAEECFTKND+IFANRP+FAVS CLSYN TTLG
Sbjct: 61  QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG 120

Query: 121 AAPYGDHWRKLRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIYEASRDDFGKVEFKSMV 180
           AAPYGDHWRKLRRVS LEIL S+RLN N  IRRDEVRR ++KI+E SRD FGKVEFK +V
Sbjct: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAVKKIFEVSRDGFGKVEFKPLV 180

Query: 181 KELTLNITMRMVAGKRYYGEEAAKSSEARTFQAIVNELFEFTLSSYPADFLPILKYIDIQ 240
           KELT+NITMRMVAGKRYYGEEAAKSSEARTFQAIV+ELFEFTLSSYPADFLPILKYIDIQ
Sbjct: 181 KELTMNITMRMVAGKRYYGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 240

Query: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDIIKG 300
           GFMKRAKKLI RVDAFWQGLIDEHRRGEISNEEM+N MVAHFLKLQE+QP+YYTDDIIKG
Sbjct: 241 GFMKRAKKLIARVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 300

Query: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAELESQLGEDQLLEETDLSKLSYLR 360
           LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAE+ESQL  D+LLEETDLSKLSYLR
Sbjct: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLDGDKLLEETDLSKLSYLR 360

Query: 361 CIVLETLRLYPAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMSFKP 420
           CIVLETLRLYPAGPLLLPHKSSADC+I GY++P DTILLVNAWAIHRDPTLWEDP SF+P
Sbjct: 361 CIVLETLRLYPAGPLLLPHKSSADCSISGYNIPRDTILLVNAWAIHRDPTLWEDPTSFRP 420

Query: 421 ERFENNNGEGDGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKEVDM 480
           ERFENNNGEGD ++KLMIAFGLGRRACPGTGMAHRV+GL LG LIQCFDWKSI+GKEVDM
Sbjct: 421 ERFENNNGEGDANNKLMIAFGLGRRACPGTGMAHRVMGLMLGTLIQCFDWKSIDGKEVDM 480

Query: 481 TEGIGVSMPKAQPLEALCKAREIATKLL 508
            EG GVSMPKAQPLEALCKAREIATKLL
Sbjct: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 508

[Close]

BLAST of Bhi12G002101 vs. ExPASy TrEMBL
Match: A0A1S3CAW8 (isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103498910 PE=3 SV=1)

HSP 1 Score: 901.7 bits (2329), Expect = 1.4e-258
Identity = 450/508 (88.58%), Postives = 477/508 (93.90%), Query Frame = 0


Query: 1   MEDSILAYSAISILSIG-IVLGIIKHLLKSNCYKNLPPCPPSIPIIGHLHLLKLPVHRTL 60
           MEDSIL YSA SILSIG +VL IIK+LLK N  KNLPP PPSIPIIGHLHLLKLPVHRTL
Sbjct: 1   MEDSILQYSAFSILSIGVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 60

Query: 61  QTLSQKYGPVLSLRFGSRLVVVVSSMQAAEECFTKNDIIFANRPDFAVSRCLSYNHTTLG 120
           QTLSQKYGPVLSLRFGSRLVVVV+S++AAEECFTKND+IFANRP+FAVS CLSYN TTLG
Sbjct: 61  QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG 120

Query: 121 AAPYGDHWRKLRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIYEASRDDFGKVEFKSMV 180
           AAPYGDHWRKLRRVS LEIL S+RLN N  IRRDEVRR ++KI+E SRD FGKVEFK +V
Sbjct: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAVKKIFEVSRDGFGKVEFKPLV 180

Query: 181 KELTLNITMRMVAGKRYYGEEAAKSSEARTFQAIVNELFEFTLSSYPADFLPILKYIDIQ 240
           KELT+NITMRMVAGKRYYGEEAAKSSEARTFQAIV+ELFEFTLSSYPADFLPILKYIDIQ
Sbjct: 181 KELTMNITMRMVAGKRYYGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 240

Query: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDIIKG 300
           GFMKRAKKLI RVDAFWQGLIDEHRRGEISNEEM+N MVAHFLKLQE+QP+YYTDDIIKG
Sbjct: 241 GFMKRAKKLIARVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 300

Query: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAELESQLGEDQLLEETDLSKLSYLR 360
           LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAE+ESQL  D+LLEETDLSKLSYLR
Sbjct: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLDGDKLLEETDLSKLSYLR 360

Query: 361 CIVLETLRLYPAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMSFKP 420
           CIVLETLRLYPAGPLLLPHKSSADC+I GY++P DTILLVNAWAIHRDPTLWEDP SF+P
Sbjct: 361 CIVLETLRLYPAGPLLLPHKSSADCSISGYNIPRDTILLVNAWAIHRDPTLWEDPTSFRP 420

Query: 421 ERFENNNGEGDGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKEVDM 480
           ERFENNNGEGD ++KLMIAFGLGRRACPGTGMAHRV+GL LG LIQCFDWKSI+GKEVDM
Sbjct: 421 ERFENNNGEGDANNKLMIAFGLGRRACPGTGMAHRVMGLMLGTLIQCFDWKSIDGKEVDM 480

Query: 481 TEGIGVSMPKAQPLEALCKAREIATKLL 508
            EG GVSMPKAQPLEALCKAREIATKLL
Sbjct: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 508

[Close]

BLAST of Bhi12G002101 vs. ExPASy TrEMBL
Match: A0A097IYP1 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa6G088160 PE=2 SV=1)

HSP 1 Score: 893.3 bits (2307), Expect = 4.8e-256
Identity = 446/508 (87.80%), Postives = 474/508 (93.31%), Query Frame = 0


Query: 1   MEDSILAYSAISILSIG-IVLGIIKHLLKSNCYKNLPPCPPSIPIIGHLHLLKLPVHRTL 60
           MEDSIL YSA SILSI  +VL IIK+LLK N  KNLPP PPSIPIIGHLHLLKLPVHRTL
Sbjct: 1   MEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 60

Query: 61  QTLSQKYGPVLSLRFGSRLVVVVSSMQAAEECFTKNDIIFANRPDFAVSRCLSYNHTTLG 120
           QTLSQKYGPVLSLRFGSR VVVV+S++AAEECF KNDIIFANRP+FAVS CLSYN TTLG
Sbjct: 61  QTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLG 120

Query: 121 AAPYGDHWRKLRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIYEASRDDFGKVEFKSMV 180
           AAPYGDHWRKLRRVS LEIL S+RLN N  IRRDEVRR M+KIYE SRD FGKVEFK +V
Sbjct: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLV 180

Query: 181 KELTLNITMRMVAGKRYYGEEAAKSSEARTFQAIVNELFEFTLSSYPADFLPILKYIDIQ 240
           KELT+N+TMRMVAGKRY+GEEAAKSSEARTFQAIV+ELFEFTLSSYPADFLPILKYIDIQ
Sbjct: 181 KELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 240

Query: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDIIKG 300
           GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEM+N MVAHFLKLQE+QP+YYTDDIIKG
Sbjct: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 300

Query: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAELESQLGEDQLLEETDLSKLSYLR 360
           LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAE+ESQLG D+L+EETDLSKL+YLR
Sbjct: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLR 360

Query: 361 CIVLETLRLYPAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMSFKP 420
            I+LETLRLYPAGPLLLPHKSSADC I GYD+P DT+LLVNAWAIHRDP LWEDP SFKP
Sbjct: 361 YIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKP 420

Query: 421 ERFENNNGEGDGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKEVDM 480
           ERFENNNGEGDG++KLMIAFGLGRRACPGTGMA+RV+GL LG LIQCFDWKSI+GKEVDM
Sbjct: 421 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDM 480

Query: 481 TEGIGVSMPKAQPLEALCKAREIATKLL 508
            EG GVSMPKAQPLEALCKAREIATKLL
Sbjct: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 508

[Close]

BLAST of Bhi12G002101 vs. ExPASy TrEMBL
Match: A0A6J1H614 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111460794 PE=3 SV=1)

HSP 1 Score: 843.2 bits (2177), Expect = 5.7e-241
Identity = 421/507 (83.04%), Postives = 461/507 (90.93%), Query Frame = 0


Query: 1   MEDSILAYSAISILSIGIVLGIIKHLLKSNCYKNLPPCPPSIPIIGHLHLLKLPVHRTLQ 60
           M+DS   YSA   L I +V+  I + LK   +KNLPP PPSIPIIGHLHLLK PVHR+L 
Sbjct: 1   MDDSTFLYSA---LCIALVVA-ISYALKPKSHKNLPPTPPSIPIIGHLHLLKPPVHRSLL 60

Query: 61  TLSQKYGPVLSLRFGSRLVVVVSSMQAAEECFTKNDIIFANRPDFAVSRCLSYNHTTLGA 120
           TLSQKYGPVLSLRFGSRLVVVVSS+ AAEECFTKND++FANRPDFAVS+CLSYNHTTLGA
Sbjct: 61  TLSQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGA 120

Query: 121 APYGDHWRKLRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIYEASRDDFGKVEFKSMVK 180
           APYGDHWRKLRRVSALEILS+NRLNTNS IRRDEVRR+  K+ + SRDDF +VEFKSMVK
Sbjct: 121 APYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLANKLSQTSRDDFARVEFKSMVK 180

Query: 181 ELTLNITMRMVAGKRYYGEEAAKSSEARTFQAIVNELFEFTLSSYPADFLPILKYIDIQG 240
           ELTLNITMRMVAGKRYYGEE  +SSEA TF+AIV+ELFEF L+ YPADFLPILKYIDIQG
Sbjct: 181 ELTLNITMRMVAGKRYYGEETDESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDIQG 240

Query: 241 FMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDIIKGL 300
           FMKRAKKLI RVDAFWQG++DEHR  +I N+EMENSMVAHFLKLQESQP+YYTDDIIKGL
Sbjct: 241 FMKRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGL 300

Query: 301 ILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAELESQLGEDQLLEETDLSKLSYLRC 360
           ILTM+LGGSDTTAVTIEWAMSNLLNHP+VLKKARAELE+QLG DQLLEE+++ KLSYLRC
Sbjct: 301 ILTMVLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLG-DQLLEESEVPKLSYLRC 360

Query: 361 IVLETLRLYPAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMSFKPE 420
           IVLETLRLYPAGPLLLPHKSSADCT+ GYDVP DTILLVNAWAIHRDPTLWEDP SF+PE
Sbjct: 361 IVLETLRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPE 420

Query: 421 RFENNNGEGDGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKEVDMT 480
           RFE   G+G+ +SKLMIAFGLGRRACPG+GMAHRVLGL+LGLLIQCFDWKSI+GKEVDMT
Sbjct: 421 RFE--GGDGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMT 480

Query: 481 EGIGVSMPKAQPLEALCKAREIATKLL 508
           EG GVSMPKA+PLEALCK R IA+KLL
Sbjct: 481 EGKGVSMPKAEPLEALCKPRPIASKLL 500

[Close]

BLAST of Bhi12G002101 vs. ExPASy TrEMBL
Match: A0A6J1KS03 (isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111498126 PE=3 SV=1)

HSP 1 Score: 840.1 bits (2169), Expect = 4.8e-240
Identity = 418/507 (82.45%), Postives = 461/507 (90.93%), Query Frame = 0


Query: 1   MEDSILAYSAISILSIGIVLGIIKHLLKSNCYKNLPPCPPSIPIIGHLHLLKLPVHRTLQ 60
           M+DS   Y   S+L I +V+  I + LK    KNLPP PPSIPIIGHLHLLK PVHR+L 
Sbjct: 1   MDDSTFLY---SVLCIALVVA-ISYALKPKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLL 60

Query: 61  TLSQKYGPVLSLRFGSRLVVVVSSMQAAEECFTKNDIIFANRPDFAVSRCLSYNHTTLGA 120
           TLSQKYGPVLSLRFGSRLVVVVSS+ AAEECFTKND++FANRPDFAVS+CLSYNHTTLGA
Sbjct: 61  TLSQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGA 120

Query: 121 APYGDHWRKLRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIYEASRDDFGKVEFKSMVK 180
           APYGDHWRKLRRVSALEILS+NRLNTNS IRRDEVRR++ K+ ++SRDDF +VEFKSMVK
Sbjct: 121 APYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMVK 180

Query: 181 ELTLNITMRMVAGKRYYGEEAAKSSEARTFQAIVNELFEFTLSSYPADFLPILKYIDIQG 240
           ELTLNITMRMVAGKRYYGEE  +SSEARTF+AIVNELFEF L+ YPADFLPILKYID QG
Sbjct: 181 ELTLNITMRMVAGKRYYGEETNESSEARTFRAIVNELFEFMLAQYPADFLPILKYIDFQG 240

Query: 241 FMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDIIKGL 300
           FMKRAKKLI RVDAFWQG++DEHR  ++ N+EMENSMVAHFLKLQESQP+YYTDDIIKGL
Sbjct: 241 FMKRAKKLIKRVDAFWQGIVDEHRTNQVHNQEMENSMVAHFLKLQESQPQYYTDDIIKGL 300

Query: 301 ILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAELESQLGEDQLLEETDLSKLSYLRC 360
           ILTM+LGGSDTTAVTIEWAMSNLLNHP+VLKKARAELE+QLG DQLLEE+++ KLSYLRC
Sbjct: 301 ILTMVLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLG-DQLLEESEVPKLSYLRC 360

Query: 361 IVLETLRLYPAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMSFKPE 420
           IVLETLRLYPAGPLLLPHKSSADCT+ GY+VP DTILLVNAWAIHRDPTLWEDP SF+PE
Sbjct: 361 IVLETLRLYPAGPLLLPHKSSADCTLSGYNVPRDTILLVNAWAIHRDPTLWEDPTSFRPE 420

Query: 421 RFENNNGEGDGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKEVDMT 480
           RFE   G+ + +SKLMIAFGLGRRACPG+GMAHRVLGL+LGLLIQCFDWKSI+GKEVDMT
Sbjct: 421 RFE--GGDSEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDMT 480

Query: 481 EGIGVSMPKAQPLEALCKAREIATKLL 508
           EG GVSMPKA+PLEALCK R IA+KLL
Sbjct: 481 EGKGVSMPKAEPLEALCKPRPIASKLL 500

The following BLAST results are available for this feature:

BLAST of Bhi12G002101 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G37310.1	3.6e-147	51.69	cytochrome P450, family 81, subfamily H, polypeptide 1	[more]
AT4G37370.1	3.6e-147	51.28	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]
AT4G37360.1	3.4e-145	53.21	cytochrome P450, family 81, subfamily D, polypeptide 2	[more]
AT4G37330.1	3.2e-143	50.79	cytochrome P450, family 81, subfamily D, polypeptide 4	[more]
AT4G37340.1	3.5e-142	51.76	cytochrome P450, family 81, subfamily D, polypeptide 3	[more]

BLAST of Bhi12G002101 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
W8JMU7	1.1e-153	54.13	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
P93147	2.6e-150	50.89	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2	[more]
Q6WNQ9	1.7e-149	54.31	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]
Q6WNQ8	2.5e-148	54.22	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]
Q6WNR0	1.1e-145	49.60	Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1	[more]

BLAST of Bhi12G002101 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038907094.1	4.3e-291	100.00	cytochrome P450 81Q32-like [Benincasa hispida]	[more]
XP_008459942.1	2.8e-258	88.58	PREDICTED: isoflavone 3'-hydroxylase-like [Cucumis melo] >KAA0039878.1 isoflavon...	[more]
NP_001289568.1	9.9e-256	87.80	isoflavone 2'-hydroxylase-like [Cucumis sativus] >AIT72031.1 cytochrome P450 [Cu...	[more]
KGN46394.2	9.9e-256	87.80	hypothetical protein Csa_005702 [Cucumis sativus]	[more]
KAG7025244.1	5.3e-241	83.04	Isoflavone 2'-hydroxylase [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Bhi12G002101 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DLU7	1.4e-258	88.58	Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3CAW8	1.4e-258	88.58	isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103498910 PE=3 SV=1	[more]
A0A097IYP1	4.8e-256	87.80	Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa6G088160 PE=2 SV=1	[more]
A0A6J1H614	5.7e-241	83.04	isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111460794 PE=...	[more]
A0A6J1KS03	4.8e-240	82.45	isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111498126 PE=3 ...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 360..371 score: 39.26 coord: 307..324 score: 34.96 coord: 437..446 score: 51.99 coord: 446..457 score: 31.96
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 36..488 e-value: 2.0E-91 score: 307.1
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 359..377 score: 33.12 coord: 400..424 score: 38.96 coord: 436..446 score: 48.46 coord: 296..313 score: 24.01 coord: 182..200 score: 22.23 coord: 87..108 score: 25.79 coord: 446..469 score: 34.37 coord: 63..82 score: 43.93 coord: 316..342 score: 31.88
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 28..505 e-value: 1.0E-123 score: 415.4
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 36..500
None	No IPR available	PIRSR	PIRSR000209-1	PIRSR000209-1	coord: 37..483 e-value: 3.4E-34 score: 115.5
None	No IPR available	PANTHER	PTHR24298:SF836	CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 2-RELATED	coord: 9..502
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 9..502
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 439..448

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi12M002101	Bhi12M002101	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006284	base-excision repair
biological_process	GO:0006298	mismatch repair
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005634	nucleus
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0034039	8-oxo-7,8-dihydroguanine DNA N-glycosylase activity
molecular_function	GO:0035485	adenine/guanine mispair binding
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0032357	oxidized purine DNA binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0000701	purine-specific mismatch base pair DNA N-glycosylase activity
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen