Bhi12G001733 (gene) Wax gourd (B227) v1

Overview


Name	Bhi12G001733
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Ubiquitin-conjugating enzyme family protein
Location	chr12: 61626137 .. 61629260 (+)
RNA-Seq Expression	Bhi12G001733
Synteny	Bhi12G001733

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTCGAGACACCGCCAGAACGATTAGTTTGTTTTTCCGTCACTTTCTTAAAACGACACTTCCTCCGTCGTTTTGAACAAAGGAATCTCATCTTTTATTCTCCCCTCTTCAGTTAATCATCTCTAAAAAAAATTAAAAAACAAACAAACAAACAAACATGTCTTCTCCTTCGTCTTCTCCTCTCTGTTATTCACACGACGGCGAAGACAAACACCCCGCCACCGAAGACGGCGGTGACGAACCTAACGGATCGCCACCGCCAACGCCGTTCTCCTTCAAACAGTTCGATATCGTCAGCGACTACTCCGATCACCATTACGCCGATTCAAACCTCTTCGCGAACTGCATTGGATCGGATGTCTCAAAGAAGATCATGCGAGAATGGAAGATTCTCGAAAGAAATCTTCCGGAATCGATTTTCGTTCGTGCTTACGAAGAGAGAATCGATCTTCTACGAGCAGTAATCGTAGGTCCAGCCGGAACTCCATATCACGACGGATTGTTCTTCTTCGACCTAGCTTTCACCTCGGACTATCCGAATAGTCCTCCGATGGTGTATTACCATTCCTACGGACTACGATTAAACCCTAACCTATACGAAGACGGCTACGTGTGCTTGAGTTTGATAAACACCTGGGATGGAAAAGAAGAAGAGCGATGGAATCCAGCGATTTCGACAGTTTTACAGATTTTAATATCGATTCAAGCGCTGGTGTTGAATGAGAAGCCGTATTTCAACGAACCGGAACTGGAAGAGGAGGTGGAAGCGGAGATTTACTCAGATGCATACAATAAGGAAGCGTACTTGTTCTGTTGCAAGATGATGATGAGATTGATGAAGAATCCGCCGAAGAATTACGAGGAGTTTGTGAAGAATTACTTTGGGGAGAAAGGGAAAGGGATACTGGCGGCGTGTAAAGCTTACTCCGACGGCCGTGTTAGGGTTGGGAGGTTCAATGAAAAGGAGAATAGTGAAAGTGAAAGTGATGATTGTAACAGAGCGGAGGTTTCTGCCATTTTTAAGGCGTTAATGGAGAAGATGCATCGGGATCTGCACGCAGCTTTCCTCCTCTTCTGCACCATCTAGAGGGTACGAACTAAGCCAACTAGTGAAATTGTCCCCCCCCTCTTTTTTTTTAAATTACTTAATTTAAGGAAGTCAAATACAGAAAATATTTTGGGTGTGAAGATAATTTGAAAGGAACATTGACTTATTATTTTCTTTTTTTTTTCCTTGTTAAATTAGTTAGTTTTTTTAATAAAAAAAAACCTTGTTTCAAAAGTTACTTTGATGACATGAAAATCGAGATCTAAACACCATTAACTGTTTTAAGAATTAATATTCATCGAACAATCATTAAGTAACATTATAAGGAGAAAAAATGTGGTGGTGTTAAAGTGAGTTTAGTTAGACGGTAATTGATATGGTGTTTCTCCTAAAAAGTCAAAATTCGATCCTCATCTATGGAAAAAAACGAAAAAAAAATGTTACTTAGAAAATAAATGATAAAATTTAGAGGTATTTTTTACCATATTCTTCCAAAGTTACAAAATTACTTTGGAACAAAATTCAGGATTTGAGATGGTGTGATAGTATAGACTACTCAATATTCATCGACAATATAAATATCTAAAATATTTATTGAAGGTGAAGGAAAACATTGCATTTTTTCAGAAGGAAAAAAGAAAGGATATTTTTAAAATAAATGAAAAGATTTGGGAGTTTATATGTTGTTGAACCTAAATATCTAATAAATAGAGAACTTTTGAAGAATATGTTATTAAGTTAGAAATAAAGGAAGATAAAGTACTGAAATAAGATTTGAAAAGTTTTGAGACAAAATTTATTTATTTAAAAAAAAAATTTAAACAAAATATATGAATATAAAAAAGGGGTGCAATATTTAGAAGTGGTTCTTTCTAGAAGTGGTAAATTTTAGATGTATATATGCAAAATTAGATACTATTTCATTAAAAAGGGTTAGAAAGTACAGTTCCAACTTTCAAGTATGTGCTAACTGCTAAACGTGCCATGTGATTTGAAATAAATAAATAAATAAATAAATAAATATATTATTATTATTATTATTATTATAAAAAATATTTTTTTTTTTTAAAAAAAACAAGACACTTCACAAAGCGTCTCTTGCGGCCAAAAATAAAGTGATGACTAAGGGCCAATAGGTCAATGATCCACATGGTCATTGTCAAAATTATATTTATTTTCCATTATTTTATATAGAAAAAGAACATGACCAAAATATTTTGAAAATCGTTGTGAATTATTATTGCTATGTGATTGATATTAACATAAGAAGTGATTATTTTTGGCGTTTGTTTGGTTTGGAATTTGTGACATGAAATAAACAAATCTAAATGTCAAATATTAAGAAAGTTTCTTGGACTTTTACATATTCTAAGTTAAATTGTAAACTAAATATTTCACACCAAAAGTATAGAAGTTGTAGTAATTTATACCTAAACTAACAATTGTATCGATTTAAACTTTAAATTTTTGTAAGTGTATCAATTTACACCATTCATTATGTCTCCTTTAGAAAAACTTCATGTTAAATTTATAATTGTATCAATTAAACATAGAAACTTTCATAAATAAATCAATTTAAGTCTTTTAGACAAAATTTTCTTTGAAAATCGTGTATTCATTTACTCTAATCATCCATTTATAAAATCGACATTTGAAGAAGTCTATACATGTTTGAGAATTAATGCATGAATGATTTTTTATGGTATTGTCCTTCCATTATATATCATGGATCTTTCATAGTCAATTTAAAACCTTGGTCATATTCCCAACATATTGTTTAAAATAGTACTAGACGAATTACCAGACTTAATGACATAGGACTCAACATGGCATAACAATTGGTTATACACAAACACACCAAAGTTAAACTGCGATTCACTGCCAATTTGATACAACGAGATTGCAAGGGAAGGGGACTTCCTAGAGCCATGAGTGGAAGGGCACCAATTGGCAATGCCGATCTTATGGAGAATAGCATAATTCACACTTAAGGCAGCAACAGACAATTGACTAGCTTGAGGCTAAGTAGAGCAAGTCCCTCCAGTCAATTCAGACATCAAAT

mRNA sequence

TTTCGAGACACCGCCAGAACGATTAGTTTGTTTTTCCGTCACTTTCTTAAAACGACACTTCCTCCGTCGTTTTGAACAAAGGAATCTCATCTTTTATTCTCCCCTCTTCAGTTAATCATCTCTAAAAAAAATTAAAAAACAAACAAACAAACAAACATGTCTTCTCCTTCGTCTTCTCCTCTCTGTTATTCACACGACGGCGAAGACAAACACCCCGCCACCGAAGACGGCGGTGACGAACCTAACGGATCGCCACCGCCAACGCCGTTCTCCTTCAAACAGTTCGATATCGTCAGCGACTACTCCGATCACCATTACGCCGATTCAAACCTCTTCGCGAACTGCATTGGATCGGATGTCTCAAAGAAGATCATGCGAGAATGGAAGATTCTCGAAAGAAATCTTCCGGAATCGATTTTCGTTCGTGCTTACGAAGAGAGAATCGATCTTCTACGAGCAGTAATCGTAGGTCCAGCCGGAACTCCATATCACGACGGATTGTTCTTCTTCGACCTAGCTTTCACCTCGGACTATCCGAATAGTCCTCCGATGGTGTATTACCATTCCTACGGACTACGATTAAACCCTAACCTATACGAAGACGGCTACGTGTGCTTGAGTTTGATAAACACCTGGGATGGAAAAGAAGAAGAGCGATGGAATCCAGCGATTTCGACAGTTTTACAGATTTTAATATCGATTCAAGCGCTGGTGTTGAATGAGAAGCCGTATTTCAACGAACCGGAACTGGAAGAGGAGGTGGAAGCGGAGATTTACTCAGATGCATACAATAAGGAAGCGTACTTGTTCTGTTGCAAGATGATGATGAGATTGATGAAGAATCCGCCGAAGAATTACGAGGAGTTTGTGAAGAATTACTTTGGGGAGAAAGGGAAAGGGATACTGGCGGCGTGTAAAGCTTACTCCGACGGCCGTGTTAGGGTTGGGAGGTTCAATGAAAAGGAGAATAGTGAAAGTGAAAGTGATGATTGTAACAGAGCGGAGGTTTCTGCCATTTTTAAGGCGTTAATGGAGAAGATGCATCGGGATCTGCACGCAGCTTTCCTCCTCTTCTGCACCATCTAGAGGGACTCAACATGGCATAACAATTGGTTATACACAAACACACCAAAGTTAAACTGCGATTCACTGCCAATTTGATACAACGAGATTGCAAGGGAAGGGGACTTCCTAGAGCCATGAGTGGAAGGGCACCAATTGGCAATGCCGATCTTATGGAGAATAGCATAATTCACACTTAAGGCAGCAACAGACAATTGACTAGCTTGAGGCTAAGTAGAGCAAGTCCCTCCAGTCAATTCAGACATCAAAT

Coding sequence (CDS)

ATGTCTTCTCCTTCGTCTTCTCCTCTCTGTTATTCACACGACGGCGAAGACAAACACCCCGCCACCGAAGACGGCGGTGACGAACCTAACGGATCGCCACCGCCAACGCCGTTCTCCTTCAAACAGTTCGATATCGTCAGCGACTACTCCGATCACCATTACGCCGATTCAAACCTCTTCGCGAACTGCATTGGATCGGATGTCTCAAAGAAGATCATGCGAGAATGGAAGATTCTCGAAAGAAATCTTCCGGAATCGATTTTCGTTCGTGCTTACGAAGAGAGAATCGATCTTCTACGAGCAGTAATCGTAGGTCCAGCCGGAACTCCATATCACGACGGATTGTTCTTCTTCGACCTAGCTTTCACCTCGGACTATCCGAATAGTCCTCCGATGGTGTATTACCATTCCTACGGACTACGATTAAACCCTAACCTATACGAAGACGGCTACGTGTGCTTGAGTTTGATAAACACCTGGGATGGAAAAGAAGAAGAGCGATGGAATCCAGCGATTTCGACAGTTTTACAGATTTTAATATCGATTCAAGCGCTGGTGTTGAATGAGAAGCCGTATTTCAACGAACCGGAACTGGAAGAGGAGGTGGAAGCGGAGATTTACTCAGATGCATACAATAAGGAAGCGTACTTGTTCTGTTGCAAGATGATGATGAGATTGATGAAGAATCCGCCGAAGAATTACGAGGAGTTTGTGAAGAATTACTTTGGGGAGAAAGGGAAAGGGATACTGGCGGCGTGTAAAGCTTACTCCGACGGCCGTGTTAGGGTTGGGAGGTTCAATGAAAAGGAGAATAGTGAAAGTGAAAGTGATGATTGTAACAGAGCGGAGGTTTCTGCCATTTTTAAGGCGTTAATGGAGAAGATGCATCGGGATCTGCACGCAGCTTTCCTCCTCTTCTGCACCATCTAG

Protein sequence

MSSPSSSPLCYSHDGEDKHPATEDGGDEPNGSPPPTPFSFKQFDIVSDYSDHHYADSNLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTSDYPNSPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPELEEEVEAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNYEEFVKNYFGEKGKGILAACKAYSDGRVRVGRFNEKENSESESDDCNRAEVSAIFKALMEKMHRDLHAAFLLFCTI

Homology

[Close]

BLAST of Bhi12G001733 vs. TAIR 10
Match: AT2G33770.1 (phosphate 2 )

HSP 1 Score: 259.2 bits (661), Expect = 4.1e-69
Identity = 130/269 (48.33%), Postives = 177/269 (65.80%), Query Frame = 0


Query: 40  FKQFDIVSDYSDHHY--ADSNLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERID 99
           F+QFD+V+D SDHH+  +D  L  + +     KK+ +EW  LE NLP +I+VR  EER+D
Sbjct: 633 FRQFDMVNDCSDHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVCEERMD 692

Query: 100 LLRAVIVGPAGTPYHDGLFFFDLAFTSDYPNSPPMVYYHSYGLRLNPNLYEDGYVCLSLI 159
           LLRA +VG  GTPYHDGLFFFD+     YP+ PPMV+YHS G+RLNPNLYE G VCLSL+
Sbjct: 693 LLRAALVGAPGTPYHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNLYESGRVCLSLL 752

Query: 160 NTWDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPELEEEV---EAEIYSDAYNKE 219
           NTW G   E WN   S++LQ+L+S QALVLNEKPYFNE   ++++   E E  S +YN+ 
Sbjct: 753 NTWSGSGTEVWNAGSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAEGEKNSVSYNEN 812

Query: 220 AYLFCCKMMMRLMKNPPKNYEEFVKNYFGEKGKGILAACKAYSDGRVRVGRFNEKENSES 279
           A+L  CK M+ +++ PPK++E  VK++F  + + +LAACKAY +G       N + NS +
Sbjct: 813 AFLITCKSMISMLRKPPKHFEMLVKDHFTHRAQHVLAACKAYMEGVPVGSSANLQGNSTT 872

Query: 280 ESDDCNRAEVSAIFKALMEKMHRDLHAAF 304
            S           FK ++ K++  L  AF
Sbjct: 873 NSTG---------FKIMLSKLYPKLLEAF 892

[Close]

BLAST of Bhi12G001733 vs. TAIR 10
Match: AT3G15355.1 (ubiquitin-conjugating enzyme 25 )

HSP 1 Score: 257.3 bits (656), Expect = 1.6e-68
Identity = 127/262 (48.47%), Postives = 170/262 (64.89%), Query Frame = 0


Query: 40  FKQFDIVSDYSDHHYADSNLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLL 99
           FK+FDIV D+SDHHYA     +     D +K+I  EW+ILE++LPE IFVRAYE R+DLL
Sbjct: 305 FKRFDIVEDFSDHHYASKGTTSKQHSKDWAKRIQDEWRILEKDLPEMIFVRAYESRMDLL 364

Query: 100 RAVIVGPAGTPYHDGLFFFDLAFTSDYPNSPPMVYYHSYGLRLNPNLYEDGYVCLSLINT 159
           RAVI+G  GTPYHDGLFFFD+ F   YP++PP+V+YHS GLR+NPNLY  G VCLSL+ T
Sbjct: 365 RAVIIGAQGTPYHDGLFFFDIFFPDTYPSTPPIVHYHSGGLRINPNLYNCGKVCLSLLGT 424

Query: 160 WDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPELEE---EVEAEIYSDAYNKEAY 219
           W G + E+W P  ST+LQ+L+SIQ L+LN+KPYFNEP  E        E  S AY++  +
Sbjct: 425 WSGNQREKWIPNTSTMLQVLVSIQGLILNQKPYFNEPGYERSAGSAHGESTSKAYSENTF 484

Query: 220 LFCCKMMMRLMKNPPKNYEEFVKNYFGEKGKGILAACKAYSDGR----VRVGRFNEKENS 279
           +   K M+  M+ PPK +E+F   +F      +L AC AY +G     +  G  + +ENS
Sbjct: 485 ILSLKTMVYTMRRPPKYFEDFAYGHFFSCAHDVLKACNAYRNGATPGYLVKGAPDVEENS 544

Query: 280 ESESDDCNRAEVSAIFKALMEK 295
              S    R +V+   + ++ K
Sbjct: 545 AGMSSLKFRTDVATFVETVLLK 566

[Close]

BLAST of Bhi12G001733 vs. TAIR 10
Match: AT2G16920.1 (ubiquitin-conjugating enzyme 23 )

HSP 1 Score: 253.1 bits (645), Expect = 3.0e-67
Identity = 133/272 (48.90%), Postives = 173/272 (63.60%), Query Frame = 0


Query: 39   SFKQFDIVSDYSDHHYADSNLFANCIGSDVS-----KKIMREWKILERNLPESIFVRAYE 98
            SF++FDI  D  DHH      F    G         KK+ ++WKIL+ NLP+ IFVRAYE
Sbjct: 823  SFRRFDISQDPLDHH------FLGVDGQKTKERQWFKKVDQDWKILQNNLPDGIFVRAYE 882

Query: 99   ERIDLLRAVIVGPAGTPYHDGLFFFDLAFTSDYPNSPPMVYYHSYGLRLNPNLYEDGYVC 158
            +R+DLLRAVIVG  GTPY DGLFFFD    SDYP+ PP  YYHS G RLNPNLYE+G VC
Sbjct: 883  DRMDLLRAVIVGAFGTPYQDGLFFFDFHLPSDYPSVPPSAYYHSGGWRLNPNLYEEGKVC 942

Query: 159  LSLINTWDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPELEEEV---EAEIYSDA 218
            LSL+NTW G+  E W+P  S++LQ+L+S+Q LVLN KPYFNE   +++V   E E  S  
Sbjct: 943  LSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQVGTAEGEKNSLG 1002

Query: 219  YNKEAYLFCCKMMMRLMKNPPKNYEEFVKNYFGEKGKGILAACKAYSDGRVRVGRFNEKE 278
            YN+  +L  CK MM LM+ PPK++EE +K++F ++G  IL AC AY  G + +G   +  
Sbjct: 1003 YNENTFLLNCKTMMYLMRKPPKDFEELIKDHFRKRGYYILKACDAYMKGYL-IGSLTKDA 1062

Query: 279  NSESESDDCNRAEVSAIFKALMEKMHRDLHAA 303
            +   E    N   V   FK ++ K+   L +A
Sbjct: 1063 SVIDERSSANSTSVG--FKLMLAKIAPKLFSA 1085

[Close]

BLAST of Bhi12G001733 vs. TAIR 10
Match: AT1G53025.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 243.0 bits (619), Expect = 3.1e-64
Identity = 123/268 (45.90%), Postives = 165/268 (61.57%), Query Frame = 0


Query: 1   MSSPSSSPLCYSHDGEDKHPATEDG----GDEPNGSPPPTPF--SFKQFDIVSDYSDHHY 60
           +++P+ +P  Y       HP   +      D  N       F   FK+FD V D+SDHHY
Sbjct: 199 VTNPTPNPFSYDASASSSHPIAAEPISSVQDSSNARKLKEEFLRDFKRFDTVEDFSDHHY 258

Query: 61  ADSNLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDG 120
           A     +     +  KK+  +WKILE +LPE+I VRA E R+DLLRAVI+G  GTPYHDG
Sbjct: 259 ASKGKSSKQHSKNWVKKVQADWKILENDLPEAISVRACESRMDLLRAVIIGAEGTPYHDG 318

Query: 121 LFFFDLAFTSDYPNSPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAIST 180
           LFFFD+ F   YP+ PP V+YHS GLR+NPNLY  G VCLSL+ TW G   E+W P  ST
Sbjct: 319 LFFFDIQFPDTYPSVPPNVHYHSGGLRINPNLYNCGKVCLSLLGTWAGSAREKWLPNEST 378

Query: 181 VLQILISIQALVLNEKPYFNEPELEEE---VEAEIYSDAYNKEAYLFCCKMMMRLMKNPP 240
           +LQ+L+SIQAL+LNEKPYFNEP   +       E  S  Y++  +L   K M+  ++ PP
Sbjct: 379 MLQLLVSIQALILNEKPYFNEPGYVQSAGTASGESKSKVYSENVFLLSLKTMVYSIRRPP 438

Query: 241 KNYEEFVKNYFGEKGKGILAACKAYSDG 260
           +++EE+V+N++  +   I+ AC AY  G
Sbjct: 439 QHFEEYVQNHYFVRSHDIVKACNAYKAG 466

[Close]

BLAST of Bhi12G001733 vs. TAIR 10
Match: AT1G53023.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 241.5 bits (615), Expect = 8.9e-64
Identity = 122/232 (52.59%), Postives = 156/232 (67.24%), Query Frame = 0


Query: 40  FKQFDIVSDYSDHHYADSNLFANCI---------GSDVSKKIMREWKILERNLPESIFVR 99
           FK+FD V D+SDHHYA     A              +  K I +EWKIL++NLPE+IFVR
Sbjct: 21  FKRFDTVEDFSDHHYAAEGSPAKLSLWHLKFKGRPKNWVKDIQKEWKILDKNLPETIFVR 80

Query: 100 AYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTSDYPNSPPMVYYHSYGLRLNPNLYEDG 159
           A E RIDLLRAVI+G  GTPYHDGLFFFD+ F   YP+ PP V+YHS GLR+NPNLY+ G
Sbjct: 81  ACESRIDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPKVHYHSGGLRINPNLYKCG 140

Query: 160 YVCLSLINTWDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPELEEEVE---AEIY 219
            VCLSLI+TW GK+ E+W P  ST+LQ+L+SIQAL+LNEKPY+NEP  E+ +     E Y
Sbjct: 141 KVCLSLISTWTGKKREKWLPKESTMLQLLVSIQALILNEKPYYNEPGYEKSMGTPLGESY 200

Query: 220 SDAYNKEAYLFCCKMMMRLMKNPPKNYEEFVKNYFGEKGKGILAACKAYSDG 260
           S  Y++  ++F  K M         ++EEFV+++F  +   I+ AC AY DG
Sbjct: 201 SKDYSENVFVFSLKTM---------HFEEFVRSHFFVRSHDIVKACNAYKDG 243

[Close]

BLAST of Bhi12G001733 vs. ExPASy Swiss-Prot
Match: Q8VY10 (Probable ubiquitin-conjugating enzyme E2 24 OS=Arabidopsis thaliana OX=3702 GN=UBC24 PE=1 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 5.8e-68
Identity = 130/269 (48.33%), Postives = 177/269 (65.80%), Query Frame = 0


Query: 40  FKQFDIVSDYSDHHY--ADSNLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERID 99
           F+QFD+V+D SDHH+  +D  L  + +     KK+ +EW  LE NLP +I+VR  EER+D
Sbjct: 633 FRQFDMVNDCSDHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVCEERMD 692

Query: 100 LLRAVIVGPAGTPYHDGLFFFDLAFTSDYPNSPPMVYYHSYGLRLNPNLYEDGYVCLSLI 159
           LLRA +VG  GTPYHDGLFFFD+     YP+ PPMV+YHS G+RLNPNLYE G VCLSL+
Sbjct: 693 LLRAALVGAPGTPYHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNLYESGRVCLSLL 752

Query: 160 NTWDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPELEEEV---EAEIYSDAYNKE 219
           NTW G   E WN   S++LQ+L+S QALVLNEKPYFNE   ++++   E E  S +YN+ 
Sbjct: 753 NTWSGSGTEVWNAGSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAEGEKNSVSYNEN 812

Query: 220 AYLFCCKMMMRLMKNPPKNYEEFVKNYFGEKGKGILAACKAYSDGRVRVGRFNEKENSES 279
           A+L  CK M+ +++ PPK++E  VK++F  + + +LAACKAY +G       N + NS +
Sbjct: 813 AFLITCKSMISMLRKPPKHFEMLVKDHFTHRAQHVLAACKAYMEGVPVGSSANLQGNSTT 872

Query: 280 ESDDCNRAEVSAIFKALMEKMHRDLHAAF 304
            S           FK ++ K++  L  AF
Sbjct: 873 NSTG---------FKIMLSKLYPKLLEAF 892

[Close]

BLAST of Bhi12G001733 vs. ExPASy Swiss-Prot
Match: Q9LUQ5 (Probable ubiquitin-conjugating enzyme E2 25 OS=Arabidopsis thaliana OX=3702 GN=UBC25 PE=2 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 2.2e-67
Identity = 127/262 (48.47%), Postives = 170/262 (64.89%), Query Frame = 0


Query: 40  FKQFDIVSDYSDHHYADSNLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLL 99
           FK+FDIV D+SDHHYA     +     D +K+I  EW+ILE++LPE IFVRAYE R+DLL
Sbjct: 305 FKRFDIVEDFSDHHYASKGTTSKQHSKDWAKRIQDEWRILEKDLPEMIFVRAYESRMDLL 364

Query: 100 RAVIVGPAGTPYHDGLFFFDLAFTSDYPNSPPMVYYHSYGLRLNPNLYEDGYVCLSLINT 159
           RAVI+G  GTPYHDGLFFFD+ F   YP++PP+V+YHS GLR+NPNLY  G VCLSL+ T
Sbjct: 365 RAVIIGAQGTPYHDGLFFFDIFFPDTYPSTPPIVHYHSGGLRINPNLYNCGKVCLSLLGT 424

Query: 160 WDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPELEE---EVEAEIYSDAYNKEAY 219
           W G + E+W P  ST+LQ+L+SIQ L+LN+KPYFNEP  E        E  S AY++  +
Sbjct: 425 WSGNQREKWIPNTSTMLQVLVSIQGLILNQKPYFNEPGYERSAGSAHGESTSKAYSENTF 484

Query: 220 LFCCKMMMRLMKNPPKNYEEFVKNYFGEKGKGILAACKAYSDGR----VRVGRFNEKENS 279
           +   K M+  M+ PPK +E+F   +F      +L AC AY +G     +  G  + +ENS
Sbjct: 485 ILSLKTMVYTMRRPPKYFEDFAYGHFFSCAHDVLKACNAYRNGATPGYLVKGAPDVEENS 544

Query: 280 ESESDDCNRAEVSAIFKALMEK 295
              S    R +V+   + ++ K
Sbjct: 545 AGMSSLKFRTDVATFVETVLLK 566

[Close]

BLAST of Bhi12G001733 vs. ExPASy Swiss-Prot
Match: Q9ZVX1 (Probable ubiquitin-conjugating enzyme E2 23 OS=Arabidopsis thaliana OX=3702 GN=UBC23 PE=1 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 4.2e-66
Identity = 133/272 (48.90%), Postives = 173/272 (63.60%), Query Frame = 0


Query: 39   SFKQFDIVSDYSDHHYADSNLFANCIGSDVS-----KKIMREWKILERNLPESIFVRAYE 98
            SF++FDI  D  DHH      F    G         KK+ ++WKIL+ NLP+ IFVRAYE
Sbjct: 823  SFRRFDISQDPLDHH------FLGVDGQKTKERQWFKKVDQDWKILQNNLPDGIFVRAYE 882

Query: 99   ERIDLLRAVIVGPAGTPYHDGLFFFDLAFTSDYPNSPPMVYYHSYGLRLNPNLYEDGYVC 158
            +R+DLLRAVIVG  GTPY DGLFFFD    SDYP+ PP  YYHS G RLNPNLYE+G VC
Sbjct: 883  DRMDLLRAVIVGAFGTPYQDGLFFFDFHLPSDYPSVPPSAYYHSGGWRLNPNLYEEGKVC 942

Query: 159  LSLINTWDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPELEEEV---EAEIYSDA 218
            LSL+NTW G+  E W+P  S++LQ+L+S+Q LVLN KPYFNE   +++V   E E  S  
Sbjct: 943  LSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQVGTAEGEKNSLG 1002

Query: 219  YNKEAYLFCCKMMMRLMKNPPKNYEEFVKNYFGEKGKGILAACKAYSDGRVRVGRFNEKE 278
            YN+  +L  CK MM LM+ PPK++EE +K++F ++G  IL AC AY  G + +G   +  
Sbjct: 1003 YNENTFLLNCKTMMYLMRKPPKDFEELIKDHFRKRGYYILKACDAYMKGYL-IGSLTKDA 1062

Query: 279  NSESESDDCNRAEVSAIFKALMEKMHRDLHAA 303
            +   E    N   V   FK ++ K+   L +A
Sbjct: 1063 SVIDERSSANSTSVG--FKLMLAKIAPKLFSA 1085

[Close]

BLAST of Bhi12G001733 vs. ExPASy Swiss-Prot
Match: Q9C918 (Putative ubiquitin-conjugating enzyme E2 38 OS=Arabidopsis thaliana OX=3702 GN=UBC38 PE=3 SV=2)

HSP 1 Score: 244.6 bits (623), Expect = 1.5e-63
Identity = 117/224 (52.23%), Postives = 152/224 (67.86%), Query Frame = 0


Query: 39  SFKQFDIVSDYSDHHYADSNLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDL 98
           +FK+FD V D+SDHHY      +     +  KK+  EWKIL +NLPE+IFVRA E R+DL
Sbjct: 26  NFKRFDSVEDFSDHHYVSKGKASKQHSKNWVKKVQDEWKILNQNLPETIFVRACESRMDL 85

Query: 99  LRAVIVGPAGTPYHDGLFFFDLAFTSDYPNSPPMVYYHSYGLRLNPNLYEDGYVCLSLIN 158
           LRAVI+G  GTPYHDGLFFFD+ F   YP+ PP VYYHS GLR+NPNLY  G VCLSL+ 
Sbjct: 86  LRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVYYHSGGLRINPNLYNCGKVCLSLLG 145

Query: 159 TWDGKEEERWNPAISTVLQILISIQALVLNEKPYFNEPE---LEEEVEAEIYSDAYNKEA 218
           TW G   + W P  ST+LQ+L+SIQALVLNE+PYFNEP    ++     E  S  Y++  
Sbjct: 146 TWHGNARQSWLPKESTMLQVLVSIQALVLNEQPYFNEPGYGLIKGTWLGESKSKVYSENV 205

Query: 219 YLFCCKMMMRLMKNPPKNYEEFVKNYFGEKGKGILAACKAYSDG 260
           +L   K M+  M+ PP+++EE+V+N++  +   I+ AC AY  G
Sbjct: 206 FLLSLKTMVYSMRKPPQHFEEYVQNHYFVRSHDIVKACNAYKAG 249

[Close]

BLAST of Bhi12G001733 vs. ExPASy Swiss-Prot
Match: Q8GY87 (Probable ubiquitin-conjugating enzyme E2 26 OS=Arabidopsis thaliana OX=3702 GN=UBC26 PE=2 SV=2)

HSP 1 Score: 243.0 bits (619), Expect = 4.3e-63
Identity = 123/268 (45.90%), Postives = 165/268 (61.57%), Query Frame = 0


Query: 1   MSSPSSSPLCYSHDGEDKHPATEDG----GDEPNGSPPPTPF--SFKQFDIVSDYSDHHY 60
           +++P+ +P  Y       HP   +      D  N       F   FK+FD V D+SDHHY
Sbjct: 199 VTNPTPNPFSYDASASSSHPIAAEPISSVQDSSNARKLKEEFLRDFKRFDTVEDFSDHHY 258

Query: 61  ADSNLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDG 120
           A     +     +  KK+  +WKILE +LPE+I VRA E R+DLLRAVI+G  GTPYHDG
Sbjct: 259 ASKGKSSKQHSKNWVKKVQADWKILENDLPEAISVRACESRMDLLRAVIIGAEGTPYHDG 318

Query: 121 LFFFDLAFTSDYPNSPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAIST 180
           LFFFD+ F   YP+ PP V+YHS GLR+NPNLY  G VCLSL+ TW G   E+W P  ST
Sbjct: 319 LFFFDIQFPDTYPSVPPNVHYHSGGLRINPNLYNCGKVCLSLLGTWAGSAREKWLPNEST 378

Query: 181 VLQILISIQALVLNEKPYFNEPELEEE---VEAEIYSDAYNKEAYLFCCKMMMRLMKNPP 240
           +LQ+L+SIQAL+LNEKPYFNEP   +       E  S  Y++  +L   K M+  ++ PP
Sbjct: 379 MLQLLVSIQALILNEKPYFNEPGYVQSAGTASGESKSKVYSENVFLLSLKTMVYSIRRPP 438

Query: 241 KNYEEFVKNYFGEKGKGILAACKAYSDG 260
           +++EE+V+N++  +   I+ AC AY  G
Sbjct: 439 QHFEEYVQNHYFVRSHDIVKACNAYKAG 466

[Close]

BLAST of Bhi12G001733 vs. NCBI nr
Match: XP_038907021.1 (probable ubiquitin-conjugating enzyme E2 25 [Benincasa hispida])

HSP 1 Score: 639.8 bits (1649), Expect = 1.2e-179
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0


Query: 1   MSSPSSSPLCYSHDGEDKHPATEDGGDEPNGSPPPTPFSFKQFDIVSDYSDHHYADSNLF 60
           MSSPSSSPLCYSHDGEDKHPATEDGGDEPNGSPPPTPFSFKQFDIVSDYSDHHYADSNLF
Sbjct: 1   MSSPSSSPLCYSHDGEDKHPATEDGGDEPNGSPPPTPFSFKQFDIVSDYSDHHYADSNLF 60

Query: 61  ANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDL 120
           ANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDL
Sbjct: 61  ANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDL 120

Query: 121 AFTSDYPNSPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILI 180
           AFTSDYPNSPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILI
Sbjct: 121 AFTSDYPNSPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILI 180

Query: 181 SIQALVLNEKPYFNEPELEEEVEAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNYEEFVKN 240
           SIQALVLNEKPYFNEPELEEEVEAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNYEEFVKN
Sbjct: 181 SIQALVLNEKPYFNEPELEEEVEAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNYEEFVKN 240

Query: 241 YFGEKGKGILAACKAYSDGRVRVGRFNEKENSESESDDCNRAEVSAIFKALMEKMHRDLH 300
           YFGEKGKGILAACKAYSDGRVRVGRFNEKENSESESDDCNRAEVSAIFKALMEKMHRDLH
Sbjct: 241 YFGEKGKGILAACKAYSDGRVRVGRFNEKENSESESDDCNRAEVSAIFKALMEKMHRDLH 300

Query: 301 AAFLLFCTI 310
           AAFLLFCTI
Sbjct: 301 AAFLLFCTI 309

[Close]

BLAST of Bhi12G001733 vs. NCBI nr
Match: XP_004148800.1 (probable ubiquitin-conjugating enzyme E2 25 [Cucumis sativus] >KGN47459.1 hypothetical protein Csa_021762 [Cucumis sativus])

HSP 1 Score: 527.7 bits (1358), Expect = 6.7e-146
Identity = 260/316 (82.28%), Postives = 279/316 (88.29%), Query Frame = 0


Query: 1   MSSPSSSPLCYSHDGEDKHPATEDGGDEPN---GSPPPTPFSFKQFDIVSDYSDHHYADS 60
           M+SPS  P     DGEDKHP T DGGDEPN    SP P  FSFK+FDIV+DYSDHHYADS
Sbjct: 1   MASPSLPPSPPPLDGEDKHPTTVDGGDEPNRSLSSPSPPSFSFKRFDIVTDYSDHHYADS 60

Query: 61  NLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFF 120
           NLF NCIGSDVSKKIMREWK+LERNLPESIFVRAYEERIDLLRAVIVGP+GTPYHDGLFF
Sbjct: 61  NLFPNCIGSDVSKKIMREWKVLERNLPESIFVRAYEERIDLLRAVIVGPSGTPYHDGLFF 120

Query: 121 FDLAFTSDYPNSPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQ 180
           FDLAFTSDYPN+PPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNP+ISTVLQ
Sbjct: 121 FDLAFTSDYPNTPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPSISTVLQ 180

Query: 181 ILISIQALVLNEKPYFNEPELE--EEVEAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNYE 240
           ILISIQ LVLNEKPYFNEPELE  E  + EIYSDAYNKE YL CC+MMMRLMKNP KNYE
Sbjct: 181 ILISIQGLVLNEKPYFNEPELEKDEADQGEIYSDAYNKEVYLLCCEMMMRLMKNPVKNYE 240

Query: 241 EFVKNYFGEKGKGILAACKAYSDGRVRVGRFNEKENSESES---DDCNRAEVSAIFKALM 300
           EFV+NYFG KG+ ILAAC+AYSDGRVRVGR+NEKEN+ + +   ++C R +VSAIFK LM
Sbjct: 241 EFVRNYFGGKGERILAACEAYSDGRVRVGRYNEKENNNNNNNNGENCKRGDVSAIFKTLM 300

Query: 301 EKMHRDLHAAFLLFCT 309
           EKMHRDLHAAFL FCT
Sbjct: 301 EKMHRDLHAAFLHFCT 316

[Close]

BLAST of Bhi12G001733 vs. NCBI nr
Match: XP_008458344.1 (PREDICTED: putative ubiquitin-conjugating enzyme E2 39 [Cucumis melo] >TYK02958.1 putative ubiquitin-conjugating enzyme E2 39 [Cucumis melo var. makuwa])

HSP 1 Score: 520.4 bits (1339), Expect = 1.1e-143
Identity = 264/324 (81.48%), Postives = 280/324 (86.42%), Query Frame = 0


Query: 1   MSSPSSSPLCYSHDGEDKHPATEDGGDEPN---GSPPPTP--FSFKQFDIVSDYSDHHYA 60
           M+SPS SP     DGEDKHP T DGGDEPN    SP P+P  FSFK+FDIVSDYSDHHYA
Sbjct: 1   MASPSPSP-SPPLDGEDKHPTTVDGGDEPNRSLSSPSPSPPSFSFKRFDIVSDYSDHHYA 60

Query: 61  DSNLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGL 120
           DSNLF NCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGP+GTPYHDGL
Sbjct: 61  DSNLFPNCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPSGTPYHDGL 120

Query: 121 FFFDLAFTSDYPNSPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTV 180
           FFFDLAFTSDYP +PPMVY HSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNP+ISTV
Sbjct: 121 FFFDLAFTSDYPITPPMVYCHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPSISTV 180

Query: 181 LQILISIQALVLNEKPYFNEPELEEEV---EAEIYSDAYNKEAYLFCCKMMMRLMKNPPK 240
           LQILISIQALVLNEKPYFNEPELEEE    + EIYSDAYNKE YL CC+MMMRLMKNP K
Sbjct: 181 LQILISIQALVLNEKPYFNEPELEEEEADDQGEIYSDAYNKEVYLLCCEMMMRLMKNPVK 240

Query: 241 NYEEFVKNYFGEKGKGILAACKAYSDGRVRVGRFNEKE--------NSESESDDCNRAEV 300
           NYEEFV+NYFGEKG+ ILAAC+AYSDGRVRVGR+NE E        N+ +  + C R +V
Sbjct: 241 NYEEFVRNYFGEKGERILAACEAYSDGRVRVGRYNENEKENNNINNNNNNNGETCKRGDV 300

Query: 301 SAIFKALMEKMHRDLHAAFLLFCT 309
           SAIFKALMEK HRDLHAAFL FCT
Sbjct: 301 SAIFKALMEKTHRDLHAAFLHFCT 323

[Close]

BLAST of Bhi12G001733 vs. NCBI nr
Match: XP_022999956.1 (probable ubiquitin-conjugating enzyme E2 25 [Cucurbita maxima])

HSP 1 Score: 487.6 bits (1254), Expect = 7.7e-134
Identity = 245/303 (80.86%), Postives = 264/303 (87.13%), Query Frame = 0


Query: 6   SSPLCYSHDGEDKHPATEDGGDEPNGSPPPTPFSFKQFDIVSDYSDHHYADSNLFANCIG 65
           +S LCYSH  EDK P T     EP  SPP     FK+FDIV+D+SDHHYA SNLF NCIG
Sbjct: 2   ASALCYSHHAEDKQPPTP---HEPERSPP-----FKRFDIVTDFSDHHYAHSNLFPNCIG 61

Query: 66  SDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTSD 125
           SDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAF+SD
Sbjct: 62  SDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSD 121

Query: 126 YPNSPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILISIQAL 185
           YP SPP+VYYHSYGLRLNPNLYEDGYVCLSLINTWDGK+EERWNPAISTVLQILISIQAL
Sbjct: 122 YPISPPIVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQAL 181

Query: 186 VLNEKPYFNEPELEEEVEAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNYEEFVKNYFGEK 245
           VLNE+PYFNEPEL+E  EAEIYSDAYNKEAYLFCCKM MRL++NPPKN+EEFV++YF ++
Sbjct: 182 VLNERPYFNEPELDEAAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQR 241

Query: 246 GKGILAACKAYSDGRVRVGRF--NEKEN---SESESDDCNRAEVSAIFKALMEKMHRDLH 304
           GKGILAACKAYS+GRVRVGRF  +E EN   S S S+  N   VSAIFKALMEK HRDL 
Sbjct: 242 GKGILAACKAYSEGRVRVGRFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEKTHRDLQ 296

[Close]

BLAST of Bhi12G001733 vs. NCBI nr
Match: XP_022930350.1 (probable ubiquitin-conjugating enzyme E2 25 [Cucurbita moschata])

HSP 1 Score: 487.6 bits (1254), Expect = 7.7e-134
Identity = 245/303 (80.86%), Postives = 264/303 (87.13%), Query Frame = 0


Query: 6   SSPLCYSHDGEDKHPATEDGGDEPNGSPPPTPFSFKQFDIVSDYSDHHYADSNLFANCIG 65
           +S LCYSHD EDK P T     EP  SPP     FK+FDIV+D+SDHHYA SNLF NCIG
Sbjct: 2   ASALCYSHDAEDKQPPT---ALEPERSPP-----FKRFDIVTDFSDHHYAHSNLFPNCIG 61

Query: 66  SDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTSD 125
           SDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAF+SD
Sbjct: 62  SDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSD 121

Query: 126 YPNSPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILISIQAL 185
           YP  PP+VYYHSYGLRLNPNLYEDGYVCLSLINTWDGK+EERWNPAISTVLQILISIQAL
Sbjct: 122 YPICPPIVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQAL 181

Query: 186 VLNEKPYFNEPELEEEVEAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNYEEFVKNYFGEK 245
           VLNE+PYFNEPEL+E  EAEIYSDAYNKEAYLFCCKM MRL++NPPKN+EEFV++YF ++
Sbjct: 182 VLNERPYFNEPELDEAAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQR 241

Query: 246 GKGILAACKAYSDGRVRVGRF--NEKEN---SESESDDCNRAEVSAIFKALMEKMHRDLH 304
           GKGILAACKAYS+GRVRVGRF  +E EN   S S S+  N   VSAIFKALMEK HRDL 
Sbjct: 242 GKGILAACKAYSEGRVRVGRFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEKTHRDLQ 296

[Close]

BLAST of Bhi12G001733 vs. ExPASy TrEMBL
Match: A0A0A0KF71 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G330470 PE=4 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 3.2e-146
Identity = 260/316 (82.28%), Postives = 279/316 (88.29%), Query Frame = 0


Query: 1   MSSPSSSPLCYSHDGEDKHPATEDGGDEPN---GSPPPTPFSFKQFDIVSDYSDHHYADS 60
           M+SPS  P     DGEDKHP T DGGDEPN    SP P  FSFK+FDIV+DYSDHHYADS
Sbjct: 1   MASPSLPPSPPPLDGEDKHPTTVDGGDEPNRSLSSPSPPSFSFKRFDIVTDYSDHHYADS 60

Query: 61  NLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFF 120
           NLF NCIGSDVSKKIMREWK+LERNLPESIFVRAYEERIDLLRAVIVGP+GTPYHDGLFF
Sbjct: 61  NLFPNCIGSDVSKKIMREWKVLERNLPESIFVRAYEERIDLLRAVIVGPSGTPYHDGLFF 120

Query: 121 FDLAFTSDYPNSPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQ 180
           FDLAFTSDYPN+PPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNP+ISTVLQ
Sbjct: 121 FDLAFTSDYPNTPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPSISTVLQ 180

Query: 181 ILISIQALVLNEKPYFNEPELE--EEVEAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNYE 240
           ILISIQ LVLNEKPYFNEPELE  E  + EIYSDAYNKE YL CC+MMMRLMKNP KNYE
Sbjct: 181 ILISIQGLVLNEKPYFNEPELEKDEADQGEIYSDAYNKEVYLLCCEMMMRLMKNPVKNYE 240

Query: 241 EFVKNYFGEKGKGILAACKAYSDGRVRVGRFNEKENSESES---DDCNRAEVSAIFKALM 300
           EFV+NYFG KG+ ILAAC+AYSDGRVRVGR+NEKEN+ + +   ++C R +VSAIFK LM
Sbjct: 241 EFVRNYFGGKGERILAACEAYSDGRVRVGRYNEKENNNNNNNNGENCKRGDVSAIFKTLM 300

Query: 301 EKMHRDLHAAFLLFCT 309
           EKMHRDLHAAFL FCT
Sbjct: 301 EKMHRDLHAAFLHFCT 316

[Close]

BLAST of Bhi12G001733 vs. ExPASy TrEMBL
Match: A0A5D3BVE0 (Putative ubiquitin-conjugating enzyme E2 39 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00410 PE=4 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 5.2e-144
Identity = 264/324 (81.48%), Postives = 280/324 (86.42%), Query Frame = 0


Query: 1   MSSPSSSPLCYSHDGEDKHPATEDGGDEPN---GSPPPTP--FSFKQFDIVSDYSDHHYA 60
           M+SPS SP     DGEDKHP T DGGDEPN    SP P+P  FSFK+FDIVSDYSDHHYA
Sbjct: 1   MASPSPSP-SPPLDGEDKHPTTVDGGDEPNRSLSSPSPSPPSFSFKRFDIVSDYSDHHYA 60

Query: 61  DSNLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGL 120
           DSNLF NCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGP+GTPYHDGL
Sbjct: 61  DSNLFPNCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPSGTPYHDGL 120

Query: 121 FFFDLAFTSDYPNSPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTV 180
           FFFDLAFTSDYP +PPMVY HSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNP+ISTV
Sbjct: 121 FFFDLAFTSDYPITPPMVYCHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPSISTV 180

Query: 181 LQILISIQALVLNEKPYFNEPELEEEV---EAEIYSDAYNKEAYLFCCKMMMRLMKNPPK 240
           LQILISIQALVLNEKPYFNEPELEEE    + EIYSDAYNKE YL CC+MMMRLMKNP K
Sbjct: 181 LQILISIQALVLNEKPYFNEPELEEEEADDQGEIYSDAYNKEVYLLCCEMMMRLMKNPVK 240

Query: 241 NYEEFVKNYFGEKGKGILAACKAYSDGRVRVGRFNEKE--------NSESESDDCNRAEV 300
           NYEEFV+NYFGEKG+ ILAAC+AYSDGRVRVGR+NE E        N+ +  + C R +V
Sbjct: 241 NYEEFVRNYFGEKGERILAACEAYSDGRVRVGRYNENEKENNNINNNNNNNGETCKRGDV 300

Query: 301 SAIFKALMEKMHRDLHAAFLLFCT 309
           SAIFKALMEK HRDLHAAFL FCT
Sbjct: 301 SAIFKALMEKTHRDLHAAFLHFCT 323

[Close]

BLAST of Bhi12G001733 vs. ExPASy TrEMBL
Match: A0A1S3C8W9 (putative ubiquitin-conjugating enzyme E2 39 OS=Cucumis melo OX=3656 GN=LOC103497784 PE=4 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 5.2e-144
Identity = 264/324 (81.48%), Postives = 280/324 (86.42%), Query Frame = 0


Query: 1   MSSPSSSPLCYSHDGEDKHPATEDGGDEPN---GSPPPTP--FSFKQFDIVSDYSDHHYA 60
           M+SPS SP     DGEDKHP T DGGDEPN    SP P+P  FSFK+FDIVSDYSDHHYA
Sbjct: 1   MASPSPSP-SPPLDGEDKHPTTVDGGDEPNRSLSSPSPSPPSFSFKRFDIVSDYSDHHYA 60

Query: 61  DSNLFANCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGL 120
           DSNLF NCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGP+GTPYHDGL
Sbjct: 61  DSNLFPNCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPSGTPYHDGL 120

Query: 121 FFFDLAFTSDYPNSPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTV 180
           FFFDLAFTSDYP +PPMVY HSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNP+ISTV
Sbjct: 121 FFFDLAFTSDYPITPPMVYCHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPSISTV 180

Query: 181 LQILISIQALVLNEKPYFNEPELEEEV---EAEIYSDAYNKEAYLFCCKMMMRLMKNPPK 240
           LQILISIQALVLNEKPYFNEPELEEE    + EIYSDAYNKE YL CC+MMMRLMKNP K
Sbjct: 181 LQILISIQALVLNEKPYFNEPELEEEEADDQGEIYSDAYNKEVYLLCCEMMMRLMKNPVK 240

Query: 241 NYEEFVKNYFGEKGKGILAACKAYSDGRVRVGRFNEKE--------NSESESDDCNRAEV 300
           NYEEFV+NYFGEKG+ ILAAC+AYSDGRVRVGR+NE E        N+ +  + C R +V
Sbjct: 241 NYEEFVRNYFGEKGERILAACEAYSDGRVRVGRYNENEKENNNINNNNNNNGETCKRGDV 300

Query: 301 SAIFKALMEKMHRDLHAAFLLFCT 309
           SAIFKALMEK HRDLHAAFL FCT
Sbjct: 301 SAIFKALMEKTHRDLHAAFLHFCT 323

[Close]

BLAST of Bhi12G001733 vs. ExPASy TrEMBL
Match: A0A6J1KEJ5 (probable ubiquitin-conjugating enzyme E2 25 OS=Cucurbita maxima OX=3661 GN=LOC111494283 PE=4 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 3.7e-134
Identity = 245/303 (80.86%), Postives = 264/303 (87.13%), Query Frame = 0


Query: 6   SSPLCYSHDGEDKHPATEDGGDEPNGSPPPTPFSFKQFDIVSDYSDHHYADSNLFANCIG 65
           +S LCYSH  EDK P T     EP  SPP     FK+FDIV+D+SDHHYA SNLF NCIG
Sbjct: 2   ASALCYSHHAEDKQPPTP---HEPERSPP-----FKRFDIVTDFSDHHYAHSNLFPNCIG 61

Query: 66  SDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTSD 125
           SDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAF+SD
Sbjct: 62  SDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSD 121

Query: 126 YPNSPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILISIQAL 185
           YP SPP+VYYHSYGLRLNPNLYEDGYVCLSLINTWDGK+EERWNPAISTVLQILISIQAL
Sbjct: 122 YPISPPIVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQAL 181

Query: 186 VLNEKPYFNEPELEEEVEAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNYEEFVKNYFGEK 245
           VLNE+PYFNEPEL+E  EAEIYSDAYNKEAYLFCCKM MRL++NPPKN+EEFV++YF ++
Sbjct: 182 VLNERPYFNEPELDEAAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQR 241

Query: 246 GKGILAACKAYSDGRVRVGRF--NEKEN---SESESDDCNRAEVSAIFKALMEKMHRDLH 304
           GKGILAACKAYS+GRVRVGRF  +E EN   S S S+  N   VSAIFKALMEK HRDL 
Sbjct: 242 GKGILAACKAYSEGRVRVGRFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEKTHRDLQ 296

[Close]

BLAST of Bhi12G001733 vs. ExPASy TrEMBL
Match: A0A6J1EWP5 (probable ubiquitin-conjugating enzyme E2 25 OS=Cucurbita moschata OX=3662 GN=LOC111436820 PE=4 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 3.7e-134
Identity = 245/303 (80.86%), Postives = 264/303 (87.13%), Query Frame = 0


Query: 6   SSPLCYSHDGEDKHPATEDGGDEPNGSPPPTPFSFKQFDIVSDYSDHHYADSNLFANCIG 65
           +S LCYSHD EDK P T     EP  SPP     FK+FDIV+D+SDHHYA SNLF NCIG
Sbjct: 2   ASALCYSHDAEDKQPPT---ALEPERSPP-----FKRFDIVTDFSDHHYAHSNLFPNCIG 61

Query: 66  SDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFTSD 125
           SDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAF+SD
Sbjct: 62  SDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSD 121

Query: 126 YPNSPPMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPAISTVLQILISIQAL 185
           YP  PP+VYYHSYGLRLNPNLYEDGYVCLSLINTWDGK+EERWNPAISTVLQILISIQAL
Sbjct: 122 YPICPPIVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQAL 181

Query: 186 VLNEKPYFNEPELEEEVEAEIYSDAYNKEAYLFCCKMMMRLMKNPPKNYEEFVKNYFGEK 245
           VLNE+PYFNEPEL+E  EAEIYSDAYNKEAYLFCCKM MRL++NPPKN+EEFV++YF ++
Sbjct: 182 VLNERPYFNEPELDEAAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQR 241

Query: 246 GKGILAACKAYSDGRVRVGRF--NEKEN---SESESDDCNRAEVSAIFKALMEKMHRDLH 304
           GKGILAACKAYS+GRVRVGRF  +E EN   S S S+  N   VSAIFKALMEK HRDL 
Sbjct: 242 GKGILAACKAYSEGRVRVGRFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEKTHRDLQ 296

The following BLAST results are available for this feature:

BLAST of Bhi12G001733 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G33770.1	4.1e-69	48.33	phosphate 2	[more]
AT3G15355.1	1.6e-68	48.47	ubiquitin-conjugating enzyme 25	[more]
AT2G16920.1	3.0e-67	48.90	ubiquitin-conjugating enzyme 23	[more]
AT1G53025.1	3.1e-64	45.90	Ubiquitin-conjugating enzyme family protein	[more]
AT1G53023.1	8.9e-64	52.59	Ubiquitin-conjugating enzyme family protein	[more]

BLAST of Bhi12G001733 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VY10	5.8e-68	48.33	Probable ubiquitin-conjugating enzyme E2 24 OS=Arabidopsis thaliana OX=3702 GN=U...	[more]
Q9LUQ5	2.2e-67	48.47	Probable ubiquitin-conjugating enzyme E2 25 OS=Arabidopsis thaliana OX=3702 GN=U...	[more]
Q9ZVX1	4.2e-66	48.90	Probable ubiquitin-conjugating enzyme E2 23 OS=Arabidopsis thaliana OX=3702 GN=U...	[more]
Q9C918	1.5e-63	52.23	Putative ubiquitin-conjugating enzyme E2 38 OS=Arabidopsis thaliana OX=3702 GN=U...	[more]
Q8GY87	4.3e-63	45.90	Probable ubiquitin-conjugating enzyme E2 26 OS=Arabidopsis thaliana OX=3702 GN=U...	[more]

BLAST of Bhi12G001733 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038907021.1	1.2e-179	100.00	probable ubiquitin-conjugating enzyme E2 25 [Benincasa hispida]	[more]
XP_004148800.1	6.7e-146	82.28	probable ubiquitin-conjugating enzyme E2 25 [Cucumis sativus] >KGN47459.1 hypoth...	[more]
XP_008458344.1	1.1e-143	81.48	PREDICTED: putative ubiquitin-conjugating enzyme E2 39 [Cucumis melo] >TYK02958....	[more]
XP_022999956.1	7.7e-134	80.86	probable ubiquitin-conjugating enzyme E2 25 [Cucurbita maxima]	[more]
XP_022930350.1	7.7e-134	80.86	probable ubiquitin-conjugating enzyme E2 25 [Cucurbita moschata]	[more]

BLAST of Bhi12G001733 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KF71	3.2e-146	82.28	UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G330470 PE...	[more]
A0A5D3BVE0	5.2e-144	81.48	Putative ubiquitin-conjugating enzyme E2 39 OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3C8W9	5.2e-144	81.48	putative ubiquitin-conjugating enzyme E2 39 OS=Cucumis melo OX=3656 GN=LOC103497...	[more]
A0A6J1KEJ5	3.7e-134	80.86	probable ubiquitin-conjugating enzyme E2 25 OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1EWP5	3.7e-134	80.86	probable ubiquitin-conjugating enzyme E2 25 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00212	ubc_7	coord: 70..224 e-value: 4.2E-25 score: 99.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..37
None	No IPR available	PANTHER	PTHR46116:SF19	UBIQUITIN-CONJUGATING ENZYME E2 38-RELATED	coord: 39..303
None	No IPR available	PANTHER	PTHR46116	(E3-INDEPENDENT) E2 UBIQUITIN-CONJUGATING ENZYME	coord: 39..303
IPR000608	Ubiquitin-conjugating enzyme E2	PFAM	PF00179	UQ_con	coord: 72..208 e-value: 1.1E-24 score: 86.7
IPR000608	Ubiquitin-conjugating enzyme E2	PROSITE	PS50127	UBC_2	coord: 67..224 score: 37.324005
IPR000608	Ubiquitin-conjugating enzyme E2	CDD	cd00195	UBCc	coord: 69..240 e-value: 2.51404E-38 score: 130.785
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	GENE3D	3.10.110.10	Ubiquitin Conjugating Enzyme	coord: 35..301 e-value: 4.2E-80 score: 271.1
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	SUPERFAMILY	54495	UBC-like	coord: 56..231

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi12M001733	Bhi12M001733	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0061631	ubiquitin conjugating enzyme activity