Bhi12G001398 (gene) Wax gourd (B227) v1

Overview


Name	Bhi12G001398
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	4,4'-diapophytoene desaturase (4,4'-diaponeurosporene-forming)
Location	chr12: 51173502 .. 51173675 (-)
RNA-Seq Expression	Bhi12G001398
Synteny	Bhi12G001398

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGTGGCGGTGGTCGGAGCAGGGATCAACGGTTTGGTTTCGGCTTATGTTCTAGCCAAAGCTGGAGTAGAGGTCGTGTTGTTTGAGAAGGAAGAGTATTTAGGTGGCCATTATAAAACTGTAAACTACGATGGCTTTTATTTGGACCTCGGCTTCATGGTCTTCAATCCT

mRNA sequence

ATGAAGGTGGCGGTGGTCGGAGCAGGGATCAACGGTTTGGTTTCGGCTTATGTTCTAGCCAAAGCTGGAGTAGAGGTCGTGTTGTTTGAGAAGGAAGAGTATTTAGGTGGCCATTATAAAACTGTAAACTACGATGGCTTTTATTTGGACCTCGGCTTCATGGTCTTCAATCCT

Coding sequence (CDS)

ATGAAGGTGGCGGTGGTCGGAGCAGGGATCAACGGTTTGGTTTCGGCTTATGTTCTAGCCAAAGCTGGAGTAGAGGTCGTGTTGTTTGAGAAGGAAGAGTATTTAGGTGGCCATTATAAAACTGTAAACTACGATGGCTTTTATTTGGACCTCGGCTTCATGGTCTTCAATCCT

Protein sequence

MKVAVVGAGINGLVSAYVLAKAGVEVVLFEKEEYLGGHYKTVNYDGFYLDLGFMVFNP

Homology

[Close]

BLAST of Bhi12G001398 vs. TAIR 10
Match: AT3G23530.1 (Cyclopropane-fatty-acyl-phospholipid synthase )

HSP 1 Score: 80.9 bits (198), Expect = 3.8e-16
Identity = 44/58 (75.86%), Postives = 49/58 (84.48%), Query Frame = 0


Query: 1  MKVAVVGAGINGLVSAYVLAKAGV-EVVLFEKEEYLGGHYKTVNYDGFYLDLGFMVFN 58
          MKVAV+G+GI+GL SAYVLA  GV EVVL+EKEE LGGH KTV +DG  LDLGFMVFN
Sbjct: 1  MKVAVIGSGISGLGSAYVLANQGVKEVVLYEKEESLGGHAKTVRFDGVDLDLGFMVFN 58

[Close]

BLAST of Bhi12G001398 vs. TAIR 10
Match: AT3G23510.1 (Cyclopropane-fatty-acyl-phospholipid synthase )

HSP 1 Score: 80.9 bits (198), Expect = 3.8e-16
Identity = 44/58 (75.86%), Postives = 49/58 (84.48%), Query Frame = 0


Query: 1  MKVAVVGAGINGLVSAYVLAKAGV-EVVLFEKEEYLGGHYKTVNYDGFYLDLGFMVFN 58
          MKVAV+G+GI+GL SAYVLA  GV EVVL+EKEE LGGH KTV +DG  LDLGFMVFN
Sbjct: 1  MKVAVIGSGISGLGSAYVLANQGVKEVVLYEKEESLGGHAKTVRFDGVDLDLGFMVFN 58

[Close]

BLAST of Bhi12G001398 vs. TAIR 10
Match: AT5G14220.1 (Flavin containing amine oxidoreductase family )

HSP 1 Score: 43.5 bits (101), Expect = 6.7e-05
Identity = 22/51 (43.14%), Postives = 33/51 (64.71%), Query Frame = 0


Query: 2  KVAVVGAGINGLVSAYVLAKAGVEVVLFEKEEYLGGHYKTVNYDGFYLDLG 53
          +VAVVGAG++GL +AY L   G+ V +FE +  +GG  ++V  +G   D G
Sbjct: 18 RVAVVGAGVSGLAAAYKLKSRGLNVTVFEADGRVGGKLRSVMQNGLIWDEG 68

[Close]

BLAST of Bhi12G001398 vs. TAIR 10
Match: AT5G14220.2 (Flavin containing amine oxidoreductase family )

HSP 1 Score: 43.5 bits (101), Expect = 6.7e-05
Identity = 22/51 (43.14%), Postives = 33/51 (64.71%), Query Frame = 0


Query: 2  KVAVVGAGINGLVSAYVLAKAGVEVVLFEKEEYLGGHYKTVNYDGFYLDLG 53
          +VAVVGAG++GL +AY L   G+ V +FE +  +GG  ++V  +G   D G
Sbjct: 18 RVAVVGAGVSGLAAAYKLKSRGLNVTVFEADGRVGGKLRSVMQNGLIWDEG 68

[Close]

BLAST of Bhi12G001398 vs. TAIR 10
Match: AT3G09580.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 42.7 bits (99), Expect = 1.1e-04
Identity = 21/54 (38.89%), Postives = 32/54 (59.26%), Query Frame = 0


Query: 3   VAVVGAGINGLVSAYVLAKAGVEVVLFEKEEYLGGHYKTVNYDGFYLDLGFMVF 57
           V ++GAG+ GL +A  L    +  +L E  + +GG  +T   DGF+LD GF +F
Sbjct: 49  VIIIGAGLAGLAAANQLTSKRIPFLLLEASDGVGGRVRTDIVDGFFLDRGFQIF 102

[Close]

BLAST of Bhi12G001398 vs. ExPASy Swiss-Prot
Match: P81383 (L-amino-acid oxidase OS=Ophiophagus hannah OX=8665 PE=1 SV=3)

HSP 1 Score: 50.8 bits (120), Expect = 5.9e-06
Identity = 25/53 (47.17%), Postives = 37/53 (69.81%), Query Frame = 0


Query: 2   KVAVVGAGINGLVSAYVLAKAGVEVVLFEKEEYLGGHYKTVNYDGFYLDLGFM 55
           K+ +VGAGI+GL +A +  +AG EVV+ E  + +GG  KT   DG+Y+D+G M
Sbjct: 53  KIVIVGAGISGLTAAKLFREAGHEVVILEASDRVGGRIKTHREDGWYVDVGPM 105

[Close]

BLAST of Bhi12G001398 vs. ExPASy Swiss-Prot
Match: Q2FDU6 (4,4'-diapophytoene desaturase (4,4'-diaponeurosporene-forming) OS=Staphylococcus aureus (strain USA300) OX=367830 GN=crtN PE=3 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 7.7e-06
Identity = 24/52 (46.15%), Postives = 33/52 (63.46%), Query Frame = 0


Query: 1  MKVAVVGAGINGLVSAYVLAKAGVEVVLFEKEEYLGGHYKTVNYDGFYLDLG 53
          MK+AV+GAG+ GL +A  +A  G EV +FEK   +GG    +  DGF  D+G
Sbjct: 1  MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKKDGFTFDMG 52

[Close]

BLAST of Bhi12G001398 vs. ExPASy Swiss-Prot
Match: Q2FV60 (4,4'-diapophytoene desaturase (4,4'-diaponeurosporene-forming) OS=Staphylococcus aureus (strain NCTC 8325 / PS 47) OX=93061 GN=crtN PE=3 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 7.7e-06
Identity = 24/52 (46.15%), Postives = 33/52 (63.46%), Query Frame = 0


Query: 1  MKVAVVGAGINGLVSAYVLAKAGVEVVLFEKEEYLGGHYKTVNYDGFYLDLG 53
          MK+AV+GAG+ GL +A  +A  G EV +FEK   +GG    +  DGF  D+G
Sbjct: 1  MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKKDGFTFDMG 52

[Close]

BLAST of Bhi12G001398 vs. ExPASy Swiss-Prot
Match: O07855 (4,4'-diapophytoene desaturase (4,4'-diaponeurosporene-forming) OS=Staphylococcus aureus (strain Newman) OX=426430 GN=crtN PE=1 SV=2)

HSP 1 Score: 50.4 bits (119), Expect = 7.7e-06
Identity = 24/52 (46.15%), Postives = 33/52 (63.46%), Query Frame = 0


Query: 1  MKVAVVGAGINGLVSAYVLAKAGVEVVLFEKEEYLGGHYKTVNYDGFYLDLG 53
          MK+AV+GAG+ GL +A  +A  G EV +FEK   +GG    +  DGF  D+G
Sbjct: 1  MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKKDGFTFDMG 52

[Close]

BLAST of Bhi12G001398 vs. ExPASy Swiss-Prot
Match: Q99R76 (4,4'-diapophytoene desaturase (4,4'-diaponeurosporene-forming) OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) OX=158878 GN=crtN PE=3 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 7.7e-06
Identity = 24/52 (46.15%), Postives = 33/52 (63.46%), Query Frame = 0


Query: 1  MKVAVVGAGINGLVSAYVLAKAGVEVVLFEKEEYLGGHYKTVNYDGFYLDLG 53
          MK+AV+GAG+ GL +A  +A  G EV +FEK   +GG    +  DGF  D+G
Sbjct: 1  MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKKDGFTFDMG 52

[Close]

BLAST of Bhi12G001398 vs. NCBI nr
Match: XP_038875013.1 (zeta-carotene desaturase-like [Benincasa hispida])

HSP 1 Score: 117.1 bits (292), Expect = 5.1e-23
Identity = 58/58 (100.00%), Postives = 58/58 (100.00%), Query Frame = 0


Query: 1  MKVAVVGAGINGLVSAYVLAKAGVEVVLFEKEEYLGGHYKTVNYDGFYLDLGFMVFNP 59
          MKVAVVGAGINGLVSAYVLAKAGVEVVLFEKEEYLGGHYKTVNYDGFYLDLGFMVFNP
Sbjct: 1  MKVAVVGAGINGLVSAYVLAKAGVEVVLFEKEEYLGGHYKTVNYDGFYLDLGFMVFNP 58

[Close]

BLAST of Bhi12G001398 vs. NCBI nr
Match: XP_038875015.1 (uncharacterized protein LOC120067537 [Benincasa hispida])

HSP 1 Score: 117.1 bits (292), Expect = 5.1e-23
Identity = 58/58 (100.00%), Postives = 58/58 (100.00%), Query Frame = 0


Query: 1  MKVAVVGAGINGLVSAYVLAKAGVEVVLFEKEEYLGGHYKTVNYDGFYLDLGFMVFNP 59
          MKVAVVGAGINGLVSAYVLAKAGVEVVLFEKEEYLGGHYKTVNYDGFYLDLGFMVFNP
Sbjct: 1  MKVAVVGAGINGLVSAYVLAKAGVEVVLFEKEEYLGGHYKTVNYDGFYLDLGFMVFNP 58

[Close]

BLAST of Bhi12G001398 vs. NCBI nr
Match: XP_038875017.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC120067539 [Benincasa hispida])

HSP 1 Score: 100.1 bits (248), Expect = 6.5e-18
Identity = 51/57 (89.47%), Postives = 54/57 (94.74%), Query Frame = 0


Query: 1  MKVAVVGAGINGLVSAYVLAKAGVEVVLFEKEEYLGGHYKTVNYDGFYLDLGFMVFN 58
          MKVAVVGAG +GLVSAYVLAKAGVEVVLFEKE+YLGGH KTVN+DGF LDLGFMVFN
Sbjct: 1  MKVAVVGAGXSGLVSAYVLAKAGVEVVLFEKEDYLGGHSKTVNFDGFDLDLGFMVFN 57

[Close]

BLAST of Bhi12G001398 vs. NCBI nr
Match: KAG1361485.1 (putative Cyclopropane fatty acid synthase [Cocos nucifera])

HSP 1 Score: 98.6 bits (244), Expect = 1.9e-17
Identity = 47/57 (82.46%), Postives = 55/57 (96.49%), Query Frame = 0


Query: 1  MKVAVVGAGINGLVSAYVLAKAGVEVVLFEKEEYLGGHYKTVNYDGFYLDLGFMVFN 58
          M+VA+VGAGI+GLVSAYVLAKAGV+VVL+EKE+YLGGH +TVN+DG YLDLGFMVFN
Sbjct: 1  MRVAIVGAGISGLVSAYVLAKAGVDVVLYEKEDYLGGHARTVNFDGIYLDLGFMVFN 57

[Close]

BLAST of Bhi12G001398 vs. NCBI nr
Match: KAG7996653.1 (hypothetical protein I3843_01G172300 [Carya illinoinensis] >KAG7996654.1 hypothetical protein I3843_01G172300 [Carya illinoinensis] >KAG7996655.1 hypothetical protein I3843_01G172300 [Carya illinoinensis] >KAG7996656.1 hypothetical protein I3843_01G172300 [Carya illinoinensis])

HSP 1 Score: 96.7 bits (239), Expect = 7.1e-17
Identity = 48/57 (84.21%), Postives = 54/57 (94.74%), Query Frame = 0


Query: 1  MKVAVVGAGINGLVSAYVLAKAGVEVVLFEKEEYLGGHYKTVNYDGFYLDLGFMVFN 58
          M+VAV+GAGI+GLVSAYVLAKAGV+VVL+EKEEYLGGH KTVN+DG  LDLGFMVFN
Sbjct: 4  MRVAVIGAGISGLVSAYVLAKAGVDVVLYEKEEYLGGHAKTVNFDGVDLDLGFMVFN 60

[Close]

BLAST of Bhi12G001398 vs. ExPASy TrEMBL
Match: A0A6I9SA72 (uncharacterized protein LOC105055750 isoform X1 OS=Elaeis guineensis var. tenera OX=51953 GN=LOC105055750 PE=4 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 4.5e-17
Identity = 46/57 (80.70%), Postives = 55/57 (96.49%), Query Frame = 0


Query: 1  MKVAVVGAGINGLVSAYVLAKAGVEVVLFEKEEYLGGHYKTVNYDGFYLDLGFMVFN 58
          M+VA+VGAGI+GLVSAYVLAKAGV+VVL+EKE+YLGGH +TVN+DG +LDLGFMVFN
Sbjct: 1  MRVAIVGAGISGLVSAYVLAKAGVDVVLYEKEDYLGGHARTVNFDGVHLDLGFMVFN 57

[Close]

BLAST of Bhi12G001398 vs. ExPASy TrEMBL
Match: A0A5A7U4B4 (Mycolic acid cyclopropane synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold560G00260 PE=4 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 4.5e-17
Identity = 47/59 (79.66%), Postives = 55/59 (93.22%), Query Frame = 0


Query: 1  MKVAVVGAGINGLVSAYVLAKAGVEVVLFEKEEYLGG-HYKTVNYDGFYLDLGFMVFNP 59
          MKVAV+GAGINGL+SAYV+AKAGVEVVLFEKEEYLG  H++T+N+DGF LDLG M+FNP
Sbjct: 1  MKVAVIGAGINGLISAYVVAKAGVEVVLFEKEEYLGSHHFRTINFDGFDLDLGIMLFNP 59

[Close]

BLAST of Bhi12G001398 vs. ExPASy TrEMBL
Match: A0A5D3B982 (Cyclopropane-fatty-acyl-phospholipid synthase isoform 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold726G00140 PE=4 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 4.5e-17
Identity = 47/59 (79.66%), Postives = 55/59 (93.22%), Query Frame = 0


Query: 1  MKVAVVGAGINGLVSAYVLAKAGVEVVLFEKEEYLGG-HYKTVNYDGFYLDLGFMVFNP 59
          MKVAV+GAGINGL+SAYV+AKAGVEVVLFEKEEYLG  H++T+N+DGF LDLG M+FNP
Sbjct: 1  MKVAVIGAGINGLISAYVVAKAGVEVVLFEKEEYLGSHHFRTINFDGFDLDLGIMLFNP 59

[Close]

BLAST of Bhi12G001398 vs. ExPASy TrEMBL
Match: A0A5A7U8M1 (Mycolic acid cyclopropane synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold560G00430 PE=4 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 4.5e-17
Identity = 47/59 (79.66%), Postives = 55/59 (93.22%), Query Frame = 0


Query: 1  MKVAVVGAGINGLVSAYVLAKAGVEVVLFEKEEYLGG-HYKTVNYDGFYLDLGFMVFNP 59
          MKVAV+GAGINGL+SAYV+AKAGVEVVLFEKEEYLG  H++T+N+DGF LDLG M+FNP
Sbjct: 1  MKVAVIGAGINGLISAYVVAKAGVEVVLFEKEEYLGSHHFRTINFDGFDLDLGIMLFNP 59

[Close]

BLAST of Bhi12G001398 vs. ExPASy TrEMBL
Match: A0A2K1ZIN8 (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_008G170300 PE=4 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.0e-16
Identity = 48/57 (84.21%), Postives = 54/57 (94.74%), Query Frame = 0


Query: 1  MKVAVVGAGINGLVSAYVLAKAGVEVVLFEKEEYLGGHYKTVNYDGFYLDLGFMVFN 58
          M+VAVVGAGI+GLVSAYVLAKAGVEVVL+EKE+YLGGH KTV++DG  LDLGFMVFN
Sbjct: 1  MRVAVVGAGISGLVSAYVLAKAGVEVVLYEKEDYLGGHAKTVSFDGVDLDLGFMVFN 57

The following BLAST results are available for this feature:

BLAST of Bhi12G001398 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G23530.1	3.8e-16	75.86	Cyclopropane-fatty-acyl-phospholipid synthase	[more]
AT3G23510.1	3.8e-16	75.86	Cyclopropane-fatty-acyl-phospholipid synthase	[more]
AT5G14220.1	6.7e-05	43.14	Flavin containing amine oxidoreductase family	[more]
AT5G14220.2	6.7e-05	43.14	Flavin containing amine oxidoreductase family	[more]
AT3G09580.1	1.1e-04	38.89	FAD/NAD(P)-binding oxidoreductase family protein	[more]

BLAST of Bhi12G001398 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P81383	5.9e-06	47.17	L-amino-acid oxidase OS=Ophiophagus hannah OX=8665 PE=1 SV=3	[more]
Q2FDU6	7.7e-06	46.15	4,4'-diapophytoene desaturase (4,4'-diaponeurosporene-forming) OS=Staphylococcus...	[more]
Q2FV60	7.7e-06	46.15	4,4'-diapophytoene desaturase (4,4'-diaponeurosporene-forming) OS=Staphylococcus...	[more]
O07855	7.7e-06	46.15	4,4'-diapophytoene desaturase (4,4'-diaponeurosporene-forming) OS=Staphylococcus...	[more]
Q99R76	7.7e-06	46.15	4,4'-diapophytoene desaturase (4,4'-diaponeurosporene-forming) OS=Staphylococcus...	[more]

BLAST of Bhi12G001398 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038875013.1	5.1e-23	100.00	zeta-carotene desaturase-like [Benincasa hispida]	[more]
XP_038875015.1	5.1e-23	100.00	uncharacterized protein LOC120067537 [Benincasa hispida]	[more]
XP_038875017.1	6.5e-18	89.47	LOW QUALITY PROTEIN: uncharacterized protein LOC120067539 [Benincasa hispida]	[more]
KAG1361485.1	1.9e-17	82.46	putative Cyclopropane fatty acid synthase [Cocos nucifera]	[more]
KAG7996653.1	7.1e-17	84.21	hypothetical protein I3843_01G172300 [Carya illinoinensis] >KAG7996654.1 hypothe...	[more]

BLAST of Bhi12G001398 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6I9SA72	4.5e-17	80.70	uncharacterized protein LOC105055750 isoform X1 OS=Elaeis guineensis var. tenera...	[more]
A0A5A7U4B4	4.5e-17	79.66	Mycolic acid cyclopropane synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A5D3B982	4.5e-17	79.66	Cyclopropane-fatty-acyl-phospholipid synthase isoform 5 OS=Cucumis melo var. mak...	[more]
A0A5A7U8M1	4.5e-17	79.66	Mycolic acid cyclopropane synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A2K1ZIN8	1.0e-16	84.21	Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_008G170300 PE=4 ...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00419	ADXRDTASE	coord: 2..24 score: 45.96 coord: 25..38 score: 29.59
None	No IPR available	PFAM	PF13450	NAD_binding_8	coord: 5..58 e-value: 1.9E-15 score: 56.8
None	No IPR available	PANTHER	PTHR42923	PROTOPORPHYRINOGEN OXIDASE	coord: 1..58
None	No IPR available	PANTHER	PTHR42923:SF17	OS12G0267100 PROTEIN	coord: 1..58
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 1..58 e-value: 7.6E-16 score: 60.6
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 1..57

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi12M001398	Bhi12M001398	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity