Bhi12G001303 (gene) Wax gourd (B227) v1

Overview
NameBhi12G001303
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionDNA-directed RNA polymerase subunit beta
Locationchr12: 48501113 .. 48501346 (-)
RNA-Seq ExpressionBhi12G001303
SyntenyBhi12G001303
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGATACAGATCAAGAAATTGAATTTCAATTATTTGTGGTGGTAGAACCATTGATAAAAGAAAGAGATGCTGTATATGAATCACTACCGGGGTCCCCGGCCAGAACCACACGTGCAAGTTTCCCTACATGTGGCTCATCCGTGCTTTTCGAGGCGCTGCCTGTGACTCAGCATGGGAGAAGGGGGCGGAACTGCACACTTTCGTGTTCAGAGCATTGTCCGAGTGAATAG

mRNA sequence

ATGGAAGATACAGATCAAGAAATTGAATTTCAATTATTTGTGGTGGTAGAACCATTGATAAAAGAAAGAGATGCTGTATATGAATCACTACCGGGGTCCCCGGCCAGAACCACACGTGCAAGTTTCCCTACATGTGGCTCATCCGTGCTTTTCGAGGCGCTGCCTGTGACTCAGCATGGGAGAAGGGGGCGGAACTGCACACTTTCGTGTTCAGAGCATTGTCCGAGTGAATAG

Coding sequence (CDS)

ATGGAAGATACAGATCAAGAAATTGAATTTCAATTATTTGTGGTGGTAGAACCATTGATAAAAGAAAGAGATGCTGTATATGAATCACTACCGGGGTCCCCGGCCAGAACCACACGTGCAAGTTTCCCTACATGTGGCTCATCCGTGCTTTTCGAGGCGCTGCCTGTGACTCAGCATGGGAGAAGGGGGCGGAACTGCACACTTTCGTGTTCAGAGCATTGTCCGAGTGAATAG

Protein sequence

MEDTDQEIEFQLFVVVEPLIKERDAVYESLPGSPARTTRASFPTCGSSVLFEALPVTQHGRRGRNCTLSCSEHCPSE
Homology
[Close]
BLAST of Bhi12G001303 vs. TAIR 10
Match: ATCG00190.1 (RNA polymerase subunit beta )

HSP 1 Score: 47.8 bits (112), Expect = 4.7e-06
Identity = 26/34 (76.47%), Postives = 28/34 (82.35%), Query Frame = 0 

Query: 1  MEDTDQEIEFQLFV----VVEPLIKERDAVYESL 31
          +ED D EIEFQLFV    +VEPLIKERDAVYESL
Sbjct: 41 IEDIDHEIEFQLFVETYQLVEPLIKERDAVYESL 74

[Close]
BLAST of Bhi12G001303 vs. ExPASy Swiss-Prot
Match: A6MMT6 (DNA-directed RNA polymerase subunit beta OS=Illicium oligandrum OX=145286 GN=rpoB PE=3 SV=2)

HSP 1 Score: 52.4 bits (124), Expect = 2.7e-06
Identity = 28/34 (82.35%), Postives = 30/34 (88.24%), Query Frame = 0 

Query: 1  MEDTDQEIEFQLFV----VVEPLIKERDAVYESL 31
          MEDTDQE+EFQLFV    +VEPLIKERDAVYESL
Sbjct: 41 MEDTDQEMEFQLFVETYQLVEPLIKERDAVYESL 74

[Close]
BLAST of Bhi12G001303 vs. ExPASy Swiss-Prot
Match: A9LYH7 (DNA-directed RNA polymerase subunit beta OS=Acorus americanus OX=263995 GN=rpoB PE=3 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 3.5e-06
Identity = 28/34 (82.35%), Postives = 30/34 (88.24%), Query Frame = 0 

Query: 1  MEDTDQEIEFQLFV----VVEPLIKERDAVYESL 31
          +EDTDQEIEFQLFV    +VEPLIKERDAVYESL
Sbjct: 53 IEDTDQEIEFQLFVERYQLVEPLIKERDAVYESL 86

[Close]
BLAST of Bhi12G001303 vs. ExPASy Swiss-Prot
Match: Q3V542 (DNA-directed RNA polymerase subunit beta OS=Acorus calamus OX=4465 GN=rpoB PE=3 SV=2)

HSP 1 Score: 52.0 bits (123), Expect = 3.5e-06
Identity = 28/34 (82.35%), Postives = 30/34 (88.24%), Query Frame = 0 

Query: 1  MEDTDQEIEFQLFV----VVEPLIKERDAVYESL 31
          +EDTDQEIEFQLFV    +VEPLIKERDAVYESL
Sbjct: 41 IEDTDQEIEFQLFVERYQLVEPLIKERDAVYESL 74

[Close]
BLAST of Bhi12G001303 vs. ExPASy Swiss-Prot
Match: Q5QA72 (DNA-directed RNA polymerase subunit beta OS=Acorus gramineus OX=55184 GN=rpoB PE=3 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 3.5e-06
Identity = 28/34 (82.35%), Postives = 30/34 (88.24%), Query Frame = 0 

Query: 1  MEDTDQEIEFQLFV----VVEPLIKERDAVYESL 31
          +EDTDQEIEFQLFV    +VEPLIKERDAVYESL
Sbjct: 41 IEDTDQEIEFQLFVERYQLVEPLIKERDAVYESL 74

[Close]
BLAST of Bhi12G001303 vs. ExPASy Swiss-Prot
Match: Q8S8X9 (DNA-directed RNA polymerase subunit beta OS=Atropa belladonna OX=33113 GN=rpoB PE=3 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 3.5e-06
Identity = 28/34 (82.35%), Postives = 30/34 (88.24%), Query Frame = 0 

Query: 1  MEDTDQEIEFQLFV----VVEPLIKERDAVYESL 31
          +EDTDQEIEFQLFV    +VEPLIKERDAVYESL
Sbjct: 41 IEDTDQEIEFQLFVETYQLVEPLIKERDAVYESL 74

[Close]
BLAST of Bhi12G001303 vs. NCBI nr
Match: KAG5606558.1 (hypothetical protein H5410_028050, partial [Solanum commersonii])

HSP 1 Score: 86.3 bits (212), Expect = 1.3e-13
Identity = 39/48 (81.25%), Postives = 41/48 (85.42%), Query Frame = 0 

Query: 30 LPGSPARTTRASFPTCGSSVLFEALPVTQHGRRGRNCTLSCSEHCPSE 78
          +P SPARTT ASFP CGS VLFEALPVTQHG+ G NCTLSC EHCPSE
Sbjct: 1  IPRSPARTTCASFPACGSPVLFEALPVTQHGKMGWNCTLSCLEHCPSE 48

[Close]
BLAST of Bhi12G001303 vs. NCBI nr
Match: KAG5575582.1 (hypothetical protein H5410_055716 [Solanum commersonii])

HSP 1 Score: 84.0 bits (206), Expect = 6.4e-13
Identity = 40/50 (80.00%), Postives = 41/50 (82.00%), Query Frame = 0 

Query: 28 ESLPGSPARTTRASFPTCGSSVLFEALPVTQHGRRGRNCTLSCSEHCPSE 78
          E  PG+    TRASFP CGS VL EALPVTQHGRRGRNCTLSCSEHCPSE
Sbjct: 21 EIFPGN----TRASFPACGSPVLSEALPVTQHGRRGRNCTLSCSEHCPSE 66

[Close]
BLAST of Bhi12G001303 vs. NCBI nr
Match: YP_009745379.1 (RNA polymerase beta subunit [Primula tsiangii] >QIH29824.1 RNA polymerase beta subunit [Primula tsiangii])

HSP 1 Score: 54.7 bits (130), Expect = 4.1e-04
Identity = 32/46 (69.57%), Postives = 33/46 (71.74%), Query Frame = 0 

Query: 1  MEDTDQEIEFQLFV----VVEPLIKERDAVYESLPGSPARTTRASF 43
          MEDTDQEIEFQLFV    +VEPLIKERDAVYESL  S      A F
Sbjct: 41 MEDTDQEIEFQLFVETYQLVEPLIKERDAVYESLTYSSELYVSAGF 86

[Close]
BLAST of Bhi12G001303 vs. NCBI nr
Match: YP_009262517.1 (RNA polymerase beta subunit [Acioa guianensis] >ANI87175.1 RNA polymerase beta subunit [Acioa guianensis])

HSP 1 Score: 53.5 bits (127), Expect = 9.2e-04
Identity = 29/34 (85.29%), Postives = 30/34 (88.24%), Query Frame = 0 

Query: 1  MEDTDQEIEFQLFV----VVEPLIKERDAVYESL 31
          MEDTDQEIEFQLFV    +VEPLIKERDAVYESL
Sbjct: 41 MEDTDQEIEFQLFVETYQLVEPLIKERDAVYESL 74

[Close]
BLAST of Bhi12G001303 vs. NCBI nr
Match: YP_009640514.1 (RNA polymerase beta subunit [Olea perrieri] >QBS53179.1 RNA polymerase beta subunit [Olea perrieri])

HSP 1 Score: 53.5 bits (127), Expect = 9.2e-04
Identity = 29/34 (85.29%), Postives = 30/34 (88.24%), Query Frame = 0 

Query: 1  MEDTDQEIEFQLFV----VVEPLIKERDAVYESL 31
          MEDTDQEIEFQLFV    +VEPLIKERDAVYESL
Sbjct: 41 MEDTDQEIEFQLFVERYQLVEPLIKERDAVYESL 74

[Close]
BLAST of Bhi12G001303 vs. ExPASy TrEMBL
Match: A0A3Q7FG84 (Uncharacterized protein OS=Solanum lycopersicum OX=4081 PE=4 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 4.6e-17
Identity = 44/48 (91.67%), Postives = 44/48 (91.67%), Query Frame = 0 

Query: 30 LPGSPARTTRASFPTCGSSVLFEALPVTQHGRRGRNCTLSCSEHCPSE 78
          LPG PARTTRASFP CGS VLFEALPVTQHGRR RNCTLSCSEHCPSE
Sbjct: 19 LPGFPARTTRASFPACGSPVLFEALPVTQHGRRRRNCTLSCSEHCPSE 66

[Close]
BLAST of Bhi12G001303 vs. ExPASy TrEMBL
Match: A0A5C0Q119 (DNA-directed RNA polymerase subunit beta OS=Nasa triphylla OX=77620 GN=rpoB PE=3 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 4.5e-04
Identity = 29/34 (85.29%), Postives = 30/34 (88.24%), Query Frame = 0 

Query: 1  MEDTDQEIEFQLFV----VVEPLIKERDAVYESL 31
          MEDTDQEIEFQLFV    +VEPLIKERDAVYESL
Sbjct: 41 MEDTDQEIEFQLFVETYQLVEPLIKERDAVYESL 74

[Close]
BLAST of Bhi12G001303 vs. ExPASy TrEMBL
Match: M4GXN6 (DNA-directed RNA polymerase subunit beta OS=Francoa sonchifolia OX=23250 GN=rpoB PE=3 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 4.5e-04
Identity = 29/34 (85.29%), Postives = 30/34 (88.24%), Query Frame = 0 

Query: 1  MEDTDQEIEFQLFV----VVEPLIKERDAVYESL 31
          MEDTDQEIEFQLFV    +VEPLIKERDAVYESL
Sbjct: 41 MEDTDQEIEFQLFVETYQLVEPLIKERDAVYESL 74

[Close]
BLAST of Bhi12G001303 vs. ExPASy TrEMBL
Match: A0A291EX78 (DNA-directed RNA polymerase subunit beta OS=Sinowilsonia henryi OX=51004 GN=rpoB PE=3 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 4.5e-04
Identity = 29/34 (85.29%), Postives = 30/34 (88.24%), Query Frame = 0 

Query: 1  MEDTDQEIEFQLFV----VVEPLIKERDAVYESL 31
          MEDTDQEIEFQLFV    +VEPLIKERDAVYESL
Sbjct: 41 MEDTDQEIEFQLFVETYQLVEPLIKERDAVYESL 74

[Close]
BLAST of Bhi12G001303 vs. ExPASy TrEMBL
Match: V9NYU8 (DNA-directed RNA polymerase subunit beta OS=Melianthus villosus OX=377280 GN=rpoB PE=3 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 4.5e-04
Identity = 29/34 (85.29%), Postives = 30/34 (88.24%), Query Frame = 0 

Query: 1  MEDTDQEIEFQLFV----VVEPLIKERDAVYESL 31
          MEDTDQEIEFQLFV    +VEPLIKERDAVYESL
Sbjct: 41 MEDTDQEIEFQLFVETYQLVEPLIKERDAVYESL 74

The following BLAST results are available for this feature:
BLAST of Bhi12G001303 vs. TAIR 10
Match NameE-valueIdentityDescription
ATCG00190.14.7e-0676.47RNA polymerase subunit beta [more]
BLAST of Bhi12G001303 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
A6MMT62.7e-0682.35DNA-directed RNA polymerase subunit beta OS=Illicium oligandrum OX=145286 GN=rpo... [more]
A9LYH73.5e-0682.35DNA-directed RNA polymerase subunit beta OS=Acorus americanus OX=263995 GN=rpoB ... [more]
Q3V5423.5e-0682.35DNA-directed RNA polymerase subunit beta OS=Acorus calamus OX=4465 GN=rpoB PE=3 ... [more]
Q5QA723.5e-0682.35DNA-directed RNA polymerase subunit beta OS=Acorus gramineus OX=55184 GN=rpoB PE... [more]
Q8S8X93.5e-0682.35DNA-directed RNA polymerase subunit beta OS=Atropa belladonna OX=33113 GN=rpoB P... [more]
BLAST of Bhi12G001303 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG5606558.11.3e-1381.25hypothetical protein H5410_028050, partial [Solanum commersonii][more]
KAG5575582.16.4e-1380.00hypothetical protein H5410_055716 [Solanum commersonii][more]
YP_009745379.14.1e-0469.57RNA polymerase beta subunit [Primula tsiangii] >QIH29824.1 RNA polymerase beta s... [more]
YP_009262517.19.2e-0485.29RNA polymerase beta subunit [Acioa guianensis] >ANI87175.1 RNA polymerase beta s... [more]
YP_009640514.19.2e-0485.29RNA polymerase beta subunit [Olea perrieri] >QBS53179.1 RNA polymerase beta subu... [more]
BLAST of Bhi12G001303 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A3Q7FG844.6e-1791.67Uncharacterized protein OS=Solanum lycopersicum OX=4081 PE=4 SV=1[more]
A0A5C0Q1194.5e-0485.29DNA-directed RNA polymerase subunit beta OS=Nasa triphylla OX=77620 GN=rpoB PE=3... [more]
M4GXN64.5e-0485.29DNA-directed RNA polymerase subunit beta OS=Francoa sonchifolia OX=23250 GN=rpoB... [more]
A0A291EX784.5e-0485.29DNA-directed RNA polymerase subunit beta OS=Sinowilsonia henryi OX=51004 GN=rpoB... [more]
V9NYU84.5e-0485.29DNA-directed RNA polymerase subunit beta OS=Melianthus villosus OX=377280 GN=rpo... [more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi12M001303Bhi12M001303mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0009507 chloroplast
molecular_function GO:0003677 DNA binding
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0032549 ribonucleoside binding