Bhi12G001197 (gene) Wax gourd (B227) v1

Overview
NameBhi12G001197
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionvacuolar protein sorting-associated protein 9A-like
Locationchr12: 44663555 .. 44667564 (+)
RNA-Seq ExpressionBhi12G001197
SyntenyBhi12G001197
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAACACTGATGTGTTCTTGGGTTTGCATGACTTTCTTGAGCGCATGCGTCAACCATCTGCCTCAGATTTCGTCAAATCCATTAAAAGGTTAATTTGCTTCTTATGTGCAAATTATAGGATATTTATTGACTTCAATTAGTTTTATTGTGATATTTCCTTCAAATTTCATCTGATTTCTTTACACATTAATTACAATACTTGCTCATTGTTCCTTTTCTCAGTCCTTTGCCTGGACCTTGAGTTTTTTTCTATCTAATTCGGTTCGTTTTCTTTAAGCATGTTTTAATTAAGAGTTCCTTTTGGGGAAAATTCACATGGTTTTTGATAATTGAGATAAAATTGGACTTAGTTGTGCGAGTTAAACTCAGTGCCTGTGATTTTTAGTTGATGATGTTATTTTAACATTTGAGATAAGTATTAATTACTTAATTTGATGAAGTTGTCACTAGAGATGAATCTCCTCATTTGGGCTTTTTGTTTTAGTTGCCATCCGTTTTTATAGATTCAAATTCCTGCCCATGAAAACTAAAACCAATCTCTTCATTTTCTTCTAGAAGGAGAAAAACACTCTCATTAACGGAAATTTAGATCACATATTCTTGGTAGGAAATAACATTTTCCAATCTCTGACGGAAAAGAACTTGTAGTTAGTGCCTAGTGATGGAGTTTAGTTTTAATGTTACGTACAAGACAACCTTCAAGAGGAGGACATATTGGTTAAGGCATTGGATATAACCGATTGGTTATTAGTTGAAGATCCTTTAATATCACCTACTTGAGACAGTATAATAATTAATACCTTAAGTGACTTACCAGCTAAGAATTTGGACTATGGGCTTTGGGCAATTTCAGTTTCAAACTTGTTTTGTACACTGGGGGTTCATAAGATTTCTACGCCTAATATATACATGCATGGATGTGTATATATCTATGAGTGTGCTTCAATTTTTCGAGCTTTAGATCGATATTGTTTTGTTACTATGTACTGAAAGAGTTGGGCAGTGATTACTAACATTTTTATTCTCTCTTTAGAGTCAAGTCTTTAGCATGTTAGAGGACATTGATTGTTGAGTTTGAATAGTAATATACATATGCATAAAGTTTTGACTGAACACTTTATATTGGCAGCTTTATTGTGTCATTTTCGAATAATGCTCCCGACCCTGAAAGGGATAGTGCTTCTGTCCAAGAATTCTTTGCCAAAATGGAGGGAGCTTTCAGAGCTCATCCACTTTGGTCTGGTTGCTCAGAAGAAGAGCTGGAAAGTGCTGGTGAAGTAAGTTCACTACTTTTGAGCATGTTACATCTTTGTAATTGACAATTATCTATTTATCACCTCAATATTGAGAATGAGGCGAATAATTAATTTTCAGTGGGTAAAAGATCTGGACTAGGAGACTTCAGTTTTAAAATATAATGTATAGTACATTTGATATTTGTCAAATATAGAAGTAAAACAAACCTACCTGAAAATGAGTAAGGCACATACTGATCCCATCCTCATATATTTAACAAAATTAATAAAATTAATGATATTAAAAAAAACCAAAACCAAGATCTTTTTATCTTGGTGCATATTTTTAGCAAACTTGCAAATAGTTTTCTAAAGGGATCATTTATTGTGTGAATTTGCAATAGCACAGGCTTTACGTCCAAAGTATTTGTTGCTTTAGTGATATTTATTATTTCTAACATTTATTCTATTTTCTAATGCGCCCCTGTCTATTAAATACAGGGATTGGAGAAGTATGTCATGACAAAGTTATTTTCTCGTGTCTATGCTTCACTTGCAGATGATGTCAAAATAGATGAACAACTTTCTGAAAAAATGGCATTGATTCAACAGTTCATTAGACCAGAAAATTTGGATATCAAACCAAATTTCCAAAATGAAACATCATGGTTGGTGAGTAAAGGTCAAAGTCATCTTCATATAATTTTTGCACTATTCATCTCCTCTCTCTTAAACTTTAGATCCTCCTGATTATTTATTTGCGCATAAATCATACTTTGAAGTTGGTTCTTTTGATACATTTTGCCAGCATTTTTAAGGTTTTTTCATCAAGTGATACTCTATCCAGCGAATTTAACTGAAATTTGCAGAATATCTTCTCTCCCTTGGCTTATCATCTATGAAAGTTTTGCATTTTTTATTTGGACATTCAAGTATACATTAAAAAATGTGTCGCAAATAATTTTTCTGTAGCTTTGTTGCTTCCGTATTCTTGGGTAATGCCAAAGTGTAGATGGAATTGTTAGTGAACCAATTCTCAGATCAGCTGTTGGCTACTCATAATGAAAAGAAAAGAAGTTTCCATAAACGTTATAATTTAATGTATATTTACTTTAAATGATTATGAAAGCCCAGGTTCTGGACAATTCTCTTGAGTTATATTTTTGGTTTTCCATGAGGAATTGGTAGGAGAAGACGATGAAATTTATAACTTTTTGAGACATTGCTGTTATGGAAATATGGTTCTTAATTGGTAGGAGAGAGACGATGAAATTCATAACTTTTTGAGACATTCCTGTTATGGAAATATGGTTCTTACTCTCAACTCTGATAAAAGTTAATTCAATTTTTATTTTTTTAACACCTTGTCTTTAATAAATAAAACTTTCTTTACTGAATTAGGTCATACCTCACATTTTAGTATGTGGAATTTTCCACCATTTGAATCGTTGCAGGTCACTTATACAAGTCTCACCTACATGTTTACATTTTATTGACAGCTTGCTCAGAAAGAACTCCTCAAGATTAACATGCACAAAGCTCCAAGAGACAAACTTGTGTGTCTTCTCAGTTGTTGTAAGGTCATCAGCAACCTGCTATTTAATGCTTCTATTGCTTCAAATGAGAATCTTCCGGGGGCTGATGAATTCCTTCCCGTCCTCATTTATGTTATCATAAAGGTGATGCATAATATAATTAAATGTCTCTTTTCACACTATAGCATGTATTCTTACATGTCAGCATTTGATAATATGGTATTTTTTCATCGAACACGAACAAACTTTAGTCATCTCTATTTGGCATTAAACTGAAAAGTCGGTTGTATATACTACATAATTATTTGTTTTGTATCTTAAAACATAGCTATTGTAGTATACAATGTAGAAACTTATCACACCATGCTCATAAATGTGTTTTTCGTGCACAATCTCTTGTAGGCAAACCCTCCACAACTGCACTCAAATTTATTGTATATACAACGATACAGGGGACAATCCCGATTAACTGGAGAAGCAGCATATTTTTTCACAAACATACTCTCTGCCGAGTCATTTATCTTAAACATTGATGCAAAGGCCCTCTCAATGGAAGAAATTGAGTTCGAAAAAAACATGGAATCAGCTCAAGCACTCCTATCTGGGCTCTCTAGTGATGTTGAAGCTCTTTCTAACAAAAGTAACTTGAATGAAGGGGTTGTTCATTCATCAGAACTAGTAGAAACCAGTTCTCAAGCTTCTGTTGAGCCTGCAGTTAGACCTAAGTCCACTGAAGCGAAGCCTAGAACTGAGGCACCACTTGCCAAAGATCAGTCAGCAGTAATGGAAGCTCCATCGCTCTCGGATTTGGAGAATAAGGGAGCAGCTATTCTTTTAAATGATCAGACAGGAGGAAGGCAAGCATTCCGAGATTATCCATATTTGTTTTCTCAAGTTGGGGATCTTACATTTAAGGACGTCGAAGAGCTTCTCAGTCAATACAAACAACTTGTTTTCAAGTACGTTTCCCTTTCTAGAGGACTGAGTGTCACAACTGCATTTTCATCTTATTCAAATTCTGAAATGCATTCCCAACATCATCCCGAGTCTTTCAAAGAACCTGAAGATGTTAGAGATGTGACATCGAATGATGAGTCAGCTGGAGAGAGGCATAGGTTAATTGATGGCTCAGATGGAACCTCGCTGTTTGGAGAAGAAAATGTCAGATCAGGGTTACCACAAGACAAGGTAGTACTACCTCAACATGAAGGAAACGAGGAAACATCTCAGTCCTAG

mRNA sequence

ATGGAGAACACTGATGTGTTCTTGGGTTTGCATGACTTTCTTGAGCGCATGCGTCAACCATCTGCCTCAGATTTCGTCAAATCCATTAAAAGCTTTATTGTGTCATTTTCGAATAATGCTCCCGACCCTGAAAGGGATAGTGCTTCTGTCCAAGAATTCTTTGCCAAAATGGAGGGAGCTTTCAGAGCTCATCCACTTTGGTCTGGTTGCTCAGAAGAAGAGCTGGAAAGTGCTGGTGAAGGATTGGAGAAGTATGTCATGACAAAGTTATTTTCTCGTGTCTATGCTTCACTTGCAGATGATGTCAAAATAGATGAACAACTTTCTGAAAAAATGGCATTGATTCAACAGTTCATTAGACCAGAAAATTTGGATATCAAACCAAATTTCCAAAATGAAACATCATGGTTGCTTGCTCAGAAAGAACTCCTCAAGATTAACATGCACAAAGCTCCAAGAGACAAACTTGTGTGTCTTCTCAGTTGTTGTAAGGTCATCAGCAACCTGCTATTTAATGCTTCTATTGCTTCAAATGAGAATCTTCCGGGGGCTGATGAATTCCTTCCCGTCCTCATTTATGTTATCATAAAGGCAAACCCTCCACAACTGCACTCAAATTTATTGTATATACAACGATACAGGGGACAATCCCGATTAACTGGAGAAGCAGCATATTTTTTCACAAACATACTCTCTGCCGAGTCATTTATCTTAAACATTGATGCAAAGGCCCTCTCAATGGAAGAAATTGAGTTCGAAAAAAACATGGAATCAGCTCAAGCACTCCTATCTGGGCTCTCTAGTGATGTTGAAGCTCTTTCTAACAAAAGTAACTTGAATGAAGGGGTTGTTCATTCATCAGAACTAGTAGAAACCAGTTCTCAAGCTTCTGTTGAGCCTGCAGTTAGACCTAAGTCCACTGAAGCGAAGCCTAGAACTGAGGCACCACTTGCCAAAGATCAGTCAGCAGTAATGGAAGCTCCATCGCTCTCGGATTTGGAGAATAAGGGAGCAGCTATTCTTTTAAATGATCAGACAGGAGGAAGGCAAGCATTCCGAGATTATCCATATTTGTTTTCTCAAGTTGGGGATCTTACATTTAAGGACGTCGAAGAGCTTCTCAGTCAATACAAACAACTTGTTTTCAAGTACGTTTCCCTTTCTAGAGGACTGAGTGTCACAACTGCATTTTCATCTTATTCAAATTCTGAAATGCATTCCCAACATCATCCCGAGTCTTTCAAAGAACCTGAAGATGTTAGAGATGTGACATCGAATGATGAGTCAGCTGGAGAGAGGCATAGGTTAATTGATGGCTCAGATGGAACCTCGCTGTTTGGAGAAGAAAATGTCAGATCAGGGTTACCACAAGACAAGGTAGTACTACCTCAACATGAAGGAAACGAGGAAACATCTCAGTCCTAG

Coding sequence (CDS)

ATGGAGAACACTGATGTGTTCTTGGGTTTGCATGACTTTCTTGAGCGCATGCGTCAACCATCTGCCTCAGATTTCGTCAAATCCATTAAAAGCTTTATTGTGTCATTTTCGAATAATGCTCCCGACCCTGAAAGGGATAGTGCTTCTGTCCAAGAATTCTTTGCCAAAATGGAGGGAGCTTTCAGAGCTCATCCACTTTGGTCTGGTTGCTCAGAAGAAGAGCTGGAAAGTGCTGGTGAAGGATTGGAGAAGTATGTCATGACAAAGTTATTTTCTCGTGTCTATGCTTCACTTGCAGATGATGTCAAAATAGATGAACAACTTTCTGAAAAAATGGCATTGATTCAACAGTTCATTAGACCAGAAAATTTGGATATCAAACCAAATTTCCAAAATGAAACATCATGGTTGCTTGCTCAGAAAGAACTCCTCAAGATTAACATGCACAAAGCTCCAAGAGACAAACTTGTGTGTCTTCTCAGTTGTTGTAAGGTCATCAGCAACCTGCTATTTAATGCTTCTATTGCTTCAAATGAGAATCTTCCGGGGGCTGATGAATTCCTTCCCGTCCTCATTTATGTTATCATAAAGGCAAACCCTCCACAACTGCACTCAAATTTATTGTATATACAACGATACAGGGGACAATCCCGATTAACTGGAGAAGCAGCATATTTTTTCACAAACATACTCTCTGCCGAGTCATTTATCTTAAACATTGATGCAAAGGCCCTCTCAATGGAAGAAATTGAGTTCGAAAAAAACATGGAATCAGCTCAAGCACTCCTATCTGGGCTCTCTAGTGATGTTGAAGCTCTTTCTAACAAAAGTAACTTGAATGAAGGGGTTGTTCATTCATCAGAACTAGTAGAAACCAGTTCTCAAGCTTCTGTTGAGCCTGCAGTTAGACCTAAGTCCACTGAAGCGAAGCCTAGAACTGAGGCACCACTTGCCAAAGATCAGTCAGCAGTAATGGAAGCTCCATCGCTCTCGGATTTGGAGAATAAGGGAGCAGCTATTCTTTTAAATGATCAGACAGGAGGAAGGCAAGCATTCCGAGATTATCCATATTTGTTTTCTCAAGTTGGGGATCTTACATTTAAGGACGTCGAAGAGCTTCTCAGTCAATACAAACAACTTGTTTTCAAGTACGTTTCCCTTTCTAGAGGACTGAGTGTCACAACTGCATTTTCATCTTATTCAAATTCTGAAATGCATTCCCAACATCATCCCGAGTCTTTCAAAGAACCTGAAGATGTTAGAGATGTGACATCGAATGATGAGTCAGCTGGAGAGAGGCATAGGTTAATTGATGGCTCAGATGGAACCTCGCTGTTTGGAGAAGAAAATGTCAGATCAGGGTTACCACAAGACAAGGTAGTACTACCTCAACATGAAGGAAACGAGGAAACATCTCAGTCCTAG

Protein sequence

MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGAFRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQLSEKMALIQQFIRPENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNENLPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFILNIDAKALSMEEIEFEKNMESAQALLSGLSSDVEALSNKSNLNEGVVHSSELVETSSQASVEPAVRPKSTEAKPRTEAPLAKDQSAVMEAPSLSDLENKGAAILLNDQTGGRQAFRDYPYLFSQVGDLTFKDVEELLSQYKQLVFKYVSLSRGLSVTTAFSSYSNSEMHSQHHPESFKEPEDVRDVTSNDESAGERHRLIDGSDGTSLFGEENVRSGLPQDKVVLPQHEGNEETSQS
Homology
BLAST of Bhi12G001197 vs. TAIR 10
Match: AT3G19770.1 (Vacuolar sorting protein 9 (VPS9) domain )

HSP 1 Score: 504.2 bits (1297), Expect = 1.1e-142
Identity = 274/439 (62.41%), Postives = 335/439 (76.31%), Query Frame = 0

Query: 1   MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MENTDVFLGLHDFLERMR+PSA DFVKSIKSFIVSFSNNAPDPE+D A VQEFF+KME A
Sbjct: 1   MENTDVFLGLHDFLERMRKPSAGDFVKSIKSFIVSFSNNAPDPEKDCAMVQEFFSKMEAA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQLSEKMALIQQFIR 120
           FRAHPLWSGCSEEEL+SAG+GLEKYVMTKLF+RV+AS  ++V  DE+L +KM+L+QQFI 
Sbjct: 61  FRAHPLWSGCSEEELDSAGDGLEKYVMTKLFTRVFASNTEEVIADEKLFQKMSLVQQFIS 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN 180
           PENLDI+P FQNE+SWLLAQKEL KINM+KAPRDKLVC+L+CCKVI+NLL NASIASNEN
Sbjct: 121 PENLDIQPTFQNESSWLLAQKELQKINMYKAPRDKLVCILNCCKVINNLLLNASIASNEN 180

Query: 181 LPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFILNI 240
            PGADEFLPVLIYV IKANPPQLHSNLLYIQRYR +S+L GEAAYFFTNILSAESFI NI
Sbjct: 181 APGADEFLPVLIYVTIKANPPQLHSNLLYIQRYRRESKLVGEAAYFFTNILSAESFISNI 240

Query: 241 DAKALSMEEIEFEKNMESAQALLSGLSSDVEALSNKSNLNEGVVHSSELVETSSQASVEP 300
           DAK++S++E EFEKNMESA+A +SGL S      + S        + +  ++ +      
Sbjct: 241 DAKSISLDEAEFEKNMESARARISGLDSQTYQTGHGSAPPPRDESTLQKTQSLNPKRENT 300

Query: 301 AVRPKSTEAKPRTEAPL-AKDQSAVMEAPSLSDLENKGAAILLNDQTGGRQAFRDYPYLF 360
             + KS+++   T   L    ++ + +A S+SDLENKGA +L    T   + F++YPY+F
Sbjct: 301 LFQSKSSDSLSGTNELLNINSETPMKKAESISDLENKGATLL--KDTEPSKVFQEYPYIF 360

Query: 361 SQVGDLTFKDVEELLSQYKQLVFKYVSLSRGLSVTTAFSSYSNSEMHSQHHPESFKEPED 420
           +  GDL   DVE LL+ YKQLVFKYV L++GL   T+ +  S+S + +     + KE ED
Sbjct: 361 ASAGDLRIGDVEGLLNSYKQLVFKYVCLTKGLGDGTSLAP-SSSPLQASSGFNTSKESED 420

Query: 421 VRDVTSNDESAGERHRLID 439
            R  +S+ +   E  R +D
Sbjct: 421 HRRSSSDVQMTKETDRSVD 436

BLAST of Bhi12G001197 vs. TAIR 10
Match: AT5G09320.1 (Vacuolar sorting protein 9 (VPS9) domain )

HSP 1 Score: 460.7 bits (1184), Expect = 1.4e-129
Identity = 254/443 (57.34%), Postives = 314/443 (70.88%), Query Frame = 0

Query: 1   MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MENTDV  GLH+FL +   PSA DF+KSIKSFIVS  N APDPE+D  +VQ+FF KME A
Sbjct: 1   MENTDVVSGLHNFLSK---PSAKDFIKSIKSFIVSILNTAPDPEKDCDAVQDFFYKMESA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQLSEKMALIQQFIR 120
           FRAHPLWSGCS++EL++AG+GLEKYVMTKLF RV+AS  +DV  DE+L +K++L+QQFI 
Sbjct: 61  FRAHPLWSGCSDDELDNAGDGLEKYVMTKLFPRVFASNTEDVISDEKLFQKISLVQQFIS 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN 180
           PENLDI+P FQN+TSWLLAQKEL KINM+ APRDKL+C+L CCKVI+NLL NASIASN+N
Sbjct: 121 PENLDIQPTFQNQTSWLLAQKELQKINMYNAPRDKLMCILRCCKVINNLLLNASIASNQN 180

Query: 181 LPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFILNI 240
            PGAD+FLPVLIYV IKANPPQ HSNLLYIQRYR QS+L GEA Y FTNILSAESFI NI
Sbjct: 181 EPGADQFLPVLIYVTIKANPPQFHSNLLYIQRYRRQSKLVGEAGYLFTNILSAESFISNI 240

Query: 241 DAKALSMEEIEFEKNMESAQALLSGLSSDVEALSNKSNLNEGVVHSSELVETSSQASVEP 300
           DAK+LSM+E +FE  M+SA A LSG  S           +    H + L    +      
Sbjct: 241 DAKSLSMDEADFETKMKSAHARLSGPGSQ----------SYQTDHGAALPTAHNTKRENM 300

Query: 301 AVRPKSTEAKPRTEAPLAKDQSAVMEAPSLSDLENKGAAILLNDQTGGRQAFRDYPYLFS 360
            +  KST++   T   L+  ++ + +A  ++DLENKGAA LLND++   + F++YPY+F+
Sbjct: 301 LLHTKSTDSFSGTNETLS--ETPIKKADPITDLENKGAATLLNDRSEATKIFQEYPYMFA 360

Query: 361 QVGDLTFKDVEELLSQYKQLVFKYVSLSRGL----SVTTAFSSYSNSEMHSQHHPESFKE 420
            VGDL   DVE+LL+ YKQLVFKYV LS+GL    S+T   S    S++   H   S   
Sbjct: 361 SVGDLKIGDVEDLLNNYKQLVFKYVCLSKGLGDATSLTPCISPLQASKVSENHTTLS--- 420

Query: 421 PEDVRDVTSNDESAGERHRLIDG 440
             D +  +  D S     R + G
Sbjct: 421 -SDFQTKSETDRSVDNLFRALQG 424

BLAST of Bhi12G001197 vs. TAIR 10
Match: AT3G19770.2 (Vacuolar sorting protein 9 (VPS9) domain )

HSP 1 Score: 274.2 bits (700), Expect = 1.9e-73
Identity = 162/303 (53.47%), Postives = 209/303 (68.98%), Query Frame = 0

Query: 137 LLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNENLPGADEFLPVLIYVII 196
           + AQKEL KINM+KAPRDKLVC+L+CCKVI+NLL NASIASNEN PGADEFLPVLIYV I
Sbjct: 1   MAAQKELQKINMYKAPRDKLVCILNCCKVINNLLLNASIASNENAPGADEFLPVLIYVTI 60

Query: 197 KANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFILNIDAKALSMEEIEFEKNM 256
           KANPPQLHSNLLYIQRYR +S+L GEAAYFFTNILSAESFI NIDAK++S++E EFEKNM
Sbjct: 61  KANPPQLHSNLLYIQRYRRESKLVGEAAYFFTNILSAESFISNIDAKSISLDEAEFEKNM 120

Query: 257 ESAQALLSGLSSDVEALSNKSNLNEGVVHSSELVETSSQASVEPAVRPKSTEAKPRTEAP 316
           ESA+A +SGL S      + S        + +  ++ +        + KS+++   T   
Sbjct: 121 ESARARISGLDSQTYQTGHGSAPPPRDESTLQKTQSLNPKRENTLFQSKSSDSLSGTNEL 180

Query: 317 L-AKDQSAVMEAPSLSDLENKGAAILLNDQTGGRQAFRDYPYLFSQVGDLTFKDVEELLS 376
           L    ++ + +A S+SDLENKGA +L    T   + F++YPY+F+  GDL   DVE LL+
Sbjct: 181 LNINSETPMKKAESISDLENKGATLL--KDTEPSKVFQEYPYIFASAGDLRIGDVEGLLN 240

Query: 377 QYKQLVFKYVSLSRGLSVTTAFSSYSNSEMHSQHHPESFKEPEDVRDVTSNDESAGERHR 436
            YKQLVFKYV L++GL   T+ +  S+S + +     + KE ED R  +S+ +   E  R
Sbjct: 241 SYKQLVFKYVCLTKGLGDGTSLAP-SSSPLQASSGFNTSKESEDHRRSSSDVQMTKETDR 300

Query: 437 LID 439
            +D
Sbjct: 301 SVD 300

BLAST of Bhi12G001197 vs. ExPASy Swiss-Prot
Match: Q9LT31 (Vacuolar protein sorting-associated protein 9A OS=Arabidopsis thaliana OX=3702 GN=VPS9A PE=1 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 1.6e-141
Identity = 274/439 (62.41%), Postives = 335/439 (76.31%), Query Frame = 0

Query: 1   MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MENTDVFLGLHDFLERMR+PSA DFVKSIKSFIVSFSNNAPDPE+D A VQEFF+KME A
Sbjct: 1   MENTDVFLGLHDFLERMRKPSAGDFVKSIKSFIVSFSNNAPDPEKDCAMVQEFFSKMEAA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQLSEKMALIQQFIR 120
           FRAHPLWSGCSEEEL+SAG+GLEKYVMTKLF+RV+AS  ++V  DE+L +KM+L+QQFI 
Sbjct: 61  FRAHPLWSGCSEEELDSAGDGLEKYVMTKLFTRVFASNTEEVIADEKLFQKMSLVQQFIS 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN 180
           PENLDI+P FQNE+SWLLAQKEL KINM+KAPRDKLVC+L+CCKVI+NLL NASIASNEN
Sbjct: 121 PENLDIQPTFQNESSWLLAQKELQKINMYKAPRDKLVCILNCCKVINNLLLNASIASNEN 180

Query: 181 LPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFILNI 240
            PGADEFLPVLIYV IKANPPQLHSNLLYIQRYR +S+L GEAAYFFTNILSAESFI NI
Sbjct: 181 APGADEFLPVLIYVTIKANPPQLHSNLLYIQRYRRESKLVGEAAYFFTNILSAESFISNI 240

Query: 241 DAKALSMEEIEFEKNMESAQALLSGLSSDVEALSNKSNLNEGVVHSSELVETSSQASVEP 300
           DAK++S++E EFEKNMESA+A +SGL S      + S        + +  ++ +      
Sbjct: 241 DAKSISLDEAEFEKNMESARARISGLDSQTYQTGHGSAPPPRDESTLQKTQSLNPKRENT 300

Query: 301 AVRPKSTEAKPRTEAPL-AKDQSAVMEAPSLSDLENKGAAILLNDQTGGRQAFRDYPYLF 360
             + KS+++   T   L    ++ + +A S+SDLENKGA +L    T   + F++YPY+F
Sbjct: 301 LFQSKSSDSLSGTNELLNINSETPMKKAESISDLENKGATLL--KDTEPSKVFQEYPYIF 360

Query: 361 SQVGDLTFKDVEELLSQYKQLVFKYVSLSRGLSVTTAFSSYSNSEMHSQHHPESFKEPED 420
           +  GDL   DVE LL+ YKQLVFKYV L++GL   T+ +  S+S + +     + KE ED
Sbjct: 361 ASAGDLRIGDVEGLLNSYKQLVFKYVCLTKGLGDGTSLAP-SSSPLQASSGFNTSKESED 420

Query: 421 VRDVTSNDESAGERHRLID 439
            R  +S+ +   E  R +D
Sbjct: 421 HRRSSSDVQMTKETDRSVD 436

BLAST of Bhi12G001197 vs. ExPASy Swiss-Prot
Match: Q10NQ3 (Vacuolar protein sorting-associated protein 9A OS=Oryza sativa subsp. japonica OX=39947 GN=VPS9A PE=1 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 3.4e-128
Identity = 259/443 (58.47%), Postives = 318/443 (71.78%), Query Frame = 0

Query: 8   LGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGAFRAHPLW 67
           L  HDFLERMRQPSA+DFVKSIK FIV+FSN APDPE DSA+VQEF   MEGAFRAH  W
Sbjct: 17  LAWHDFLERMRQPSAADFVKSIKGFIVTFSNRAPDPEHDSAAVQEFLENMEGAFRAHTPW 76

Query: 68  SGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQLSEKMALIQQFIRPENLDIK 127
           +G SEEELESAGEGLEKYVMTKLF+RV+AS+ +DVK DE+L EKM+L+QQFIRPENLDIK
Sbjct: 77  AGSSEEELESAGEGLEKYVMTKLFNRVFASVPEDVKSDEELFEKMSLLQQFIRPENLDIK 136

Query: 128 PNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNENLPGADEF 187
           P +Q+ETSWLLAQKEL KINM+KAPRDKL C+L+CCKVI+NLL NASI SNEN PGADEF
Sbjct: 137 PEYQSETSWLLAQKELQKINMYKAPRDKLACILNCCKVINNLLLNASIVSNENPPGADEF 196

Query: 188 LPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFILNIDAKALSM 247
           LPVLIYV IKANPPQLHSNLLYIQRYR QSRL  EA YFFTNILSAESFI NID ++LSM
Sbjct: 197 LPVLIYVTIKANPPQLHSNLLYIQRYRRQSRLVSEAQYFFTNILSAESFIWNIDGESLSM 256

Query: 248 EEIEFEKNMESAQALLSGLSSDVEALSNKSNLNEGVVHSSELVETSSQASVEPAVRPKST 307
           +E +F+K M+ A+  + GLS+  E   N++NL+      S+ ++ S  + V  ++  K  
Sbjct: 257 DERDFQKKMDLARERMLGLSASSENQDNQNNLDVR-EQKSQTLKASRDSDVNLSL--KDN 316

Query: 308 EAKPRTEAPLAKDQSA--VMEAPSLSDLENKGAAILLNDQTGGRQAFRDYPYLFSQVGDL 367
              P  E     D S+  V    S+SDLE KGAA LL D    ++  ++YP+LF++ GDL
Sbjct: 317 FQGPGLEMRRDSDASSNPVERVQSISDLEKKGAAELLKDDDLNKK-IQEYPFLFARSGDL 376

Query: 368 TFKDVEELLSQYKQLVFKYVSLSRGLSVTTAFSSYSNSEMHSQHHPESFKEPEDVRDVTS 427
           T  DVE LL+ YKQLV KYV+LS+G+ +     +  N  + S        E E+ ++V +
Sbjct: 377 TVADVENLLNSYKQLVLKYVALSQGMGI-----NLENPPVQSMQTVSDLVESEEPKNVKN 436

Query: 428 NDESAGERHRLIDGSDGTSLFGE 449
               +    +  D     +L+ E
Sbjct: 437 AVNFSEGSSKTSDDIKNDTLYSE 450

BLAST of Bhi12G001197 vs. ExPASy Swiss-Prot
Match: O74396 (Vacuolar protein sorting-associated protein 9a OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=vps901 PE=1 SV=3)

HSP 1 Score: 164.1 bits (414), Expect = 3.9e-39
Identity = 101/310 (32.58%), Postives = 167/310 (53.87%), Query Frame = 0

Query: 2   ENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGAF 61
           E  +     H FLE++R  SA    K +KSF+  F+            +++F   +    
Sbjct: 107 EINEPIFDFHMFLEQLRSSSAEPVAKYLKSFLSEFTKRRWTVNYQVKLIRDFLKFINEKI 166

Query: 62  RAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVY------------ASLADDVKIDEQLS 121
             +  W+  S+ E+++A EG+EK V+ +L++ ++            +  +DDV+ D  LS
Sbjct: 167 EQYEPWASGSQAEIDNAKEGMEKLVLNRLYTSLFSPEIAKSGIPLSSEHSDDVEEDRVLS 226

Query: 122 EKMALIQQFIRPENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNL 181
           EKM L  Q+I  ENLDIK    +   + LA  EL +IN + APRDK++CLL+CCKVI + 
Sbjct: 227 EKMELF-QWITEENLDIKKQKSSSKFFKLAADELRRINDYHAPRDKIICLLNCCKVIFSY 286

Query: 182 LFNASIASNENLPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTN 241
           L N  +   E+   AD F+P+LI+V+++A P  L SN+ YIQR+R   +LTGE  Y+ + 
Sbjct: 287 LRN--VVKEES---ADMFVPILIFVVLQARPAHLVSNIQYIQRFRSPEKLTGEVMYYLST 346

Query: 242 ILSAESFILNIDAKALSMEEIEFEKNMESAQALLSGLSSDVEALSNKSNLNEGVVHSSEL 300
           ++ A SFI  +D  +L++ E EF   +E +   +       E   +K+ +NE   +   +
Sbjct: 347 LMGAMSFIETLDCSSLTITEEEFNAQIEKSIKKMEERKLS-EKSESKTAVNENATYKDPV 406

BLAST of Bhi12G001197 vs. ExPASy Swiss-Prot
Match: A5D794 (GTPase-activating protein and VPS9 domain-containing protein 1 OS=Bos taurus OX=9913 GN=GAPVD1 PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 5.3e-28
Identity = 76/218 (34.86%), Postives = 121/218 (55.50%), Query Frame = 0

Query: 27   KSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGAFRAHPLWSGCSEEELESAGEGLEKYV 86
            K I+ FI  F       ++ +A V++F   + GA     +W   SEE+L+ A   +E+ V
Sbjct: 1198 KKIREFIQDFQKLTAADDK-TAQVEDFLQFLYGAMAQDVIWQNASEEQLQDAQLAIERSV 1257

Query: 87   MTKLFS-RVYASLADDVKIDEQLSEKMALIQQFIRPEN--LDIKPNFQNETSWLLAQKEL 146
            M ++F    Y +   D+  D+ L E +  + + +   +  L I   +  E  W  AQ E+
Sbjct: 1258 MNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHRALQIPEVYLKEAPWPSAQSEI 1317

Query: 147  LKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNENLPGADEFLPVLIYVIIKANPPQL 206
              I+ +K PRDK+ C+L  C  I NLL   S+A+ +++PGAD+F+PVL++V+IKANPP L
Sbjct: 1318 RTISAYKTPRDKVQCILRMCSTIMNLL---SLANEDSVPGADDFVPVLVFVLIKANPPCL 1377

Query: 207  HSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFILNID 242
             S + YI  +   S L+GE +Y++    +A  FI  ID
Sbjct: 1378 LSTVQYISSFYA-SCLSGEESYWWMQFTAAVEFIKTID 1410

BLAST of Bhi12G001197 vs. ExPASy Swiss-Prot
Match: Q14C86 (GTPase-activating protein and VPS9 domain-containing protein 1 OS=Homo sapiens OX=9606 GN=GAPVD1 PE=1 SV=2)

HSP 1 Score: 127.1 bits (318), Expect = 5.3e-28
Identity = 76/218 (34.86%), Postives = 121/218 (55.50%), Query Frame = 0

Query: 27   KSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGAFRAHPLWSGCSEEELESAGEGLEKYV 86
            K I+ FI  F       ++ +A V++F   + GA     +W   SEE+L+ A   +E+ V
Sbjct: 1263 KKIREFIQDFQKLTAADDK-TAQVEDFLQFLYGAMAQDVIWQNASEEQLQDAQLAIERSV 1322

Query: 87   MTKLFS-RVYASLADDVKIDEQLSEKMALIQQFIRPEN--LDIKPNFQNETSWLLAQKEL 146
            M ++F    Y +   D+  D+ L E +  + + +   +  L I   +  E  W  AQ E+
Sbjct: 1323 MNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHRALQIPEVYLREAPWPSAQSEI 1382

Query: 147  LKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNENLPGADEFLPVLIYVIIKANPPQL 206
              I+ +K PRDK+ C+L  C  I NLL   S+A+ +++PGAD+F+PVL++V+IKANPP L
Sbjct: 1383 RTISAYKTPRDKVQCILRMCSTIMNLL---SLANEDSVPGADDFVPVLVFVLIKANPPCL 1442

Query: 207  HSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFILNID 242
             S + YI  +   S L+GE +Y++    +A  FI  ID
Sbjct: 1443 LSTVQYISSFYA-SCLSGEESYWWMQFTAAVEFIKTID 1475

BLAST of Bhi12G001197 vs. NCBI nr
Match: XP_038874663.1 (vacuolar protein sorting-associated protein 9A-like [Benincasa hispida] >XP_038874664.1 vacuolar protein sorting-associated protein 9A-like [Benincasa hispida] >XP_038874665.1 vacuolar protein sorting-associated protein 9A-like [Benincasa hispida] >XP_038874667.1 vacuolar protein sorting-associated protein 9A-like [Benincasa hispida])

HSP 1 Score: 910.2 bits (2351), Expect = 7.3e-261
Identity = 474/474 (100.00%), Postives = 474/474 (100.00%), Query Frame = 0

Query: 1   MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA
Sbjct: 1   MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQLSEKMALIQQFIR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQLSEKMALIQQFIR
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQLSEKMALIQQFIR 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN 180
           PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN
Sbjct: 121 PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN 180

Query: 181 LPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFILNI 240
           LPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFILNI
Sbjct: 181 LPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFILNI 240

Query: 241 DAKALSMEEIEFEKNMESAQALLSGLSSDVEALSNKSNLNEGVVHSSELVETSSQASVEP 300
           DAKALSMEEIEFEKNMESAQALLSGLSSDVEALSNKSNLNEGVVHSSELVETSSQASVEP
Sbjct: 241 DAKALSMEEIEFEKNMESAQALLSGLSSDVEALSNKSNLNEGVVHSSELVETSSQASVEP 300

Query: 301 AVRPKSTEAKPRTEAPLAKDQSAVMEAPSLSDLENKGAAILLNDQTGGRQAFRDYPYLFS 360
           AVRPKSTEAKPRTEAPLAKDQSAVMEAPSLSDLENKGAAILLNDQTGGRQAFRDYPYLFS
Sbjct: 301 AVRPKSTEAKPRTEAPLAKDQSAVMEAPSLSDLENKGAAILLNDQTGGRQAFRDYPYLFS 360

Query: 361 QVGDLTFKDVEELLSQYKQLVFKYVSLSRGLSVTTAFSSYSNSEMHSQHHPESFKEPEDV 420
           QVGDLTFKDVEELLSQYKQLVFKYVSLSRGLSVTTAFSSYSNSEMHSQHHPESFKEPEDV
Sbjct: 361 QVGDLTFKDVEELLSQYKQLVFKYVSLSRGLSVTTAFSSYSNSEMHSQHHPESFKEPEDV 420

Query: 421 RDVTSNDESAGERHRLIDGSDGTSLFGEENVRSGLPQDKVVLPQHEGNEETSQS 475
           RDVTSNDESAGERHRLIDGSDGTSLFGEENVRSGLPQDKVVLPQHEGNEETSQS
Sbjct: 421 RDVTSNDESAGERHRLIDGSDGTSLFGEENVRSGLPQDKVVLPQHEGNEETSQS 474

BLAST of Bhi12G001197 vs. NCBI nr
Match: XP_004138949.2 (vacuolar protein sorting-associated protein 9A isoform X1 [Cucumis sativus])

HSP 1 Score: 850.5 bits (2196), Expect = 6.9e-243
Identity = 444/474 (93.67%), Postives = 453/474 (95.57%), Query Frame = 0

Query: 1   MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA
Sbjct: 1   MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQLSEKMALIQQFIR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQ+SEKMALIQQFIR
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQISEKMALIQQFIR 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN 180
           PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN
Sbjct: 121 PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN 180

Query: 181 LPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFILNI 240
            PGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTN+LSAESFI NI
Sbjct: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNVLSAESFISNI 240

Query: 241 DAKALSMEEIEFEKNMESAQALLSGLSSDVEALSNKSNLNEGVVHSSELVETSSQASVEP 300
           DAKALSMEEIEFEKNMESA+ALLSGLSSDVEALSNK+NL+EGV H SELVETSSQASVE 
Sbjct: 241 DAKALSMEEIEFEKNMESARALLSGLSSDVEALSNKNNLDEGVGHYSELVETSSQASVES 300

Query: 301 AVRPKSTEAKPRTEAPLAKDQSAVMEAPSLSDLENKGAAILLNDQTGGRQAFRDYPYLFS 360
            VRPK TE KPRTEAP AKDQSAVM+ PSLSDLENKGA ILLNDQTGGRQA R+YPYLFS
Sbjct: 301 PVRPKPTEGKPRTEAPHAKDQSAVMKVPSLSDLENKGATILLNDQTGGRQALREYPYLFS 360

Query: 361 QVGDLTFKDVEELLSQYKQLVFKYVSLSRGLSVTTAFSSYSNSEMHSQHHPESFKEPEDV 420
           QVGDLTFKDVEELLSQYKQLVFKYVSLSRGLS+TT FSSYS SEMHSQHH ESFKEPEDV
Sbjct: 361 QVGDLTFKDVEELLSQYKQLVFKYVSLSRGLSLTTEFSSYSKSEMHSQHHHESFKEPEDV 420

Query: 421 RDVTSNDESAGERHRLIDGSDGTSLFGEENVRSGLPQDKVVLPQHEGNEETSQS 475
           RDVTSNDESAG+RHRLIDG DGTSLFGEENV SGLPQDKV LPQ EGN E SQS
Sbjct: 421 RDVTSNDESAGDRHRLIDGPDGTSLFGEENVGSGLPQDKVELPQREGNNEMSQS 474

BLAST of Bhi12G001197 vs. NCBI nr
Match: XP_008441683.2 (PREDICTED: vacuolar protein sorting-associated protein 9A-like [Cucumis melo])

HSP 1 Score: 842.0 bits (2174), Expect = 2.5e-240
Identity = 443/474 (93.46%), Postives = 450/474 (94.94%), Query Frame = 0

Query: 1   MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA
Sbjct: 29  MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 88

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQLSEKMALIQQFIR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQLSEKMALIQQFIR
Sbjct: 89  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQLSEKMALIQQFIR 148

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN 180
           PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN
Sbjct: 149 PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN 208

Query: 181 LPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFILNI 240
            PGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFI NI
Sbjct: 209 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 268

Query: 241 DAKALSMEEIEFEKNMESAQALLSGLSSDVEALSNKSNLNEGVVHSSELVETSSQASVEP 300
           DAKALSMEEIEFEKNMESA+ALLSGLSSDVEA+SNK+NL+EGV H SELVETSSQASVE 
Sbjct: 269 DAKALSMEEIEFEKNMESARALLSGLSSDVEAISNKNNLDEGVGHYSELVETSSQASVES 328

Query: 301 AVRPKSTEAKPRTEAPLAKDQSAVMEAPSLSDLENKGAAILLNDQTGGRQAFRDYPYLFS 360
            VRPK TE KPRTEAP AKDQS VME PSLSDLENKGA ILLNDQTGGRQA RDYPYLFS
Sbjct: 329 QVRPKPTEGKPRTEAPHAKDQSTVMEVPSLSDLENKGATILLNDQTGGRQALRDYPYLFS 388

Query: 361 QVGDLTFKDVEELLSQYKQLVFKYVSLSRGLSVTTAFSSYSNSEMHSQHHPESFKEPEDV 420
           QVGDLTFKDVEELLSQYKQLVFKYVSLSRGL +TT  SSYSNSEM+SQHH ESFKEPEDV
Sbjct: 389 QVGDLTFKDVEELLSQYKQLVFKYVSLSRGLGLTTELSSYSNSEMYSQHHHESFKEPEDV 448

Query: 421 RDVTSNDESAGERHRLIDGSDGTSLFGEENVRSGLPQDKVVLPQHEGNEETSQS 475
           RD+TSNDESAGE HRLIDG DGTSLFGEENV SGL  DKVVLPQHEGN E SQS
Sbjct: 449 RDMTSNDESAGEGHRLIDGPDGTSLFGEENVGSGL--DKVVLPQHEGNNEMSQS 500

BLAST of Bhi12G001197 vs. NCBI nr
Match: KAA0032359.1 (vacuolar protein sorting-associated protein 9A-like [Cucumis melo var. makuwa])

HSP 1 Score: 781.9 bits (2018), Expect = 3.0e-222
Identity = 413/444 (93.02%), Postives = 420/444 (94.59%), Query Frame = 0

Query: 31  SFIVSFSNNAPDPERDSASVQEFFAKMEGAFRAHPLWSGCSEEELESAGEGLEKYVMTKL 90
           SFIVSFSNNAPDPERDSASVQEFFAKMEGAFRAHPLWSGCSEEELESAGEGLEKYVMTKL
Sbjct: 137 SFIVSFSNNAPDPERDSASVQEFFAKMEGAFRAHPLWSGCSEEELESAGEGLEKYVMTKL 196

Query: 91  FSRVYASLADDVKIDEQLSEKMALIQQFIRPENLDIKPNFQNETSWLLAQKELLKINMHK 150
           FSRVYASLADDVKIDEQLSEKMALIQQFIRPENLDIKPNFQNETSWLLAQKELLKINMHK
Sbjct: 197 FSRVYASLADDVKIDEQLSEKMALIQQFIRPENLDIKPNFQNETSWLLAQKELLKINMHK 256

Query: 151 APRDKLVCLLSCCKVISNLLFNASIASNENLPGADEFLPVLIYVIIKANPPQLHSNLLYI 210
           APRDKLVCLLSCCKVISNLLFNASIASNEN PGADEFLPVLIYVIIKANPPQLHSNLLYI
Sbjct: 257 APRDKLVCLLSCCKVISNLLFNASIASNENPPGADEFLPVLIYVIIKANPPQLHSNLLYI 316

Query: 211 QRYRGQSRLTGEAAYFFTNILSAESFILNIDAKALSMEEIEFEKNMESAQALLSGLSSDV 270
           QRYRGQSRLTGEAAYFFTNILSAESFI NIDAKALSMEEIEFEKNMESA+ALLSGLSSDV
Sbjct: 317 QRYRGQSRLTGEAAYFFTNILSAESFISNIDAKALSMEEIEFEKNMESARALLSGLSSDV 376

Query: 271 EALSNKSNLNEGVVHSSELVETSSQASVEPAVRPKSTEAKPRTEAPLAKDQSAVMEAPSL 330
           EA+SNK+NL+EGV H SELVETSSQASVE  VRPK TE KPRTEAP AKDQS VME PSL
Sbjct: 377 EAISNKNNLDEGVGHYSELVETSSQASVESQVRPKPTEGKPRTEAPHAKDQSTVMEVPSL 436

Query: 331 SDLENKGAAILLNDQTGGRQAFRDYPYLFSQVGDLTFKDVEELLSQYKQLVFKYVSLSRG 390
           SDLENKGA ILLNDQTGGRQA RDYPYLFSQVGDLTFKDVEELLSQYKQLVFKYVSLSRG
Sbjct: 437 SDLENKGATILLNDQTGGRQALRDYPYLFSQVGDLTFKDVEELLSQYKQLVFKYVSLSRG 496

Query: 391 LSVTTAFSSYSNSEMHSQHHPESFKEPEDVRDVTSNDESAGERHRLIDGSDGTSLFGEEN 450
           L +TT  SSYSNSEM+SQHH ESFKEPEDVRD+TSNDESAGE HRLIDG DGTSLFGEEN
Sbjct: 497 LGLTTELSSYSNSEMYSQHHHESFKEPEDVRDMTSNDESAGEGHRLIDGPDGTSLFGEEN 556

Query: 451 VRSGLPQDKVVLPQHEGNEETSQS 475
           V SGL  DKVVLPQHEGN E SQS
Sbjct: 557 VGSGL--DKVVLPQHEGNNEMSQS 578

BLAST of Bhi12G001197 vs. NCBI nr
Match: XP_022141647.1 (vacuolar protein sorting-associated protein 9A-like [Momordica charantia])

HSP 1 Score: 779.6 bits (2012), Expect = 1.5e-221
Identity = 409/477 (85.74%), Postives = 438/477 (91.82%), Query Frame = 0

Query: 1   MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MEN DVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA
Sbjct: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQLSEKMALIQQFIR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKLF+RVYASL DDVKIDEQLS+K+ALIQQF R
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFTRVYASLRDDVKIDEQLSDKIALIQQFAR 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN 180
           PENLDIKPNFQNETSWLLAQKELLKINM+KAPRDKLVCLL+CCKVISNLL NAS+ASNEN
Sbjct: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASNEN 180

Query: 181 LPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFILNI 240
            PGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQ+RLTGEAAYFFTN+LSAESFI NI
Sbjct: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFISNI 240

Query: 241 DAKALSMEEIEFEKNMESAQALLSGLSSDVEALSNKSNLNEGVVHSSELVETSSQAS--- 300
           DAKALSM+EIEFE  MESA+ALLSGLS+D EA+SNKSNL+EGVV +S LV++SSQAS   
Sbjct: 241 DAKALSMDEIEFENKMESARALLSGLSTDAEAISNKSNLDEGVVRNSGLVKSSSQASNVG 300

Query: 301 VEPAVRPKSTEAKPRTEAPLAKDQSAVMEAPSLSDLENKGAAILLNDQTGGRQAFRDYPY 360
            EPAV PKS EAKPRTEA  AKDQS VME PSLSDLENKGAAILLND T GRQAFRDYPY
Sbjct: 301 TEPAVIPKSAEAKPRTEASCAKDQSTVMEEPSLSDLENKGAAILLNDHT-GRQAFRDYPY 360

Query: 361 LFSQVGDLTFKDVEELLSQYKQLVFKYVSLSRGLSVTTAFSSYSNSEMHSQHHPESFKEP 420
           LF+QVGDLTF DV+ELLSQYKQLVFKY+SLS+GLS+TT+  S S+SEM S H PESFKEP
Sbjct: 361 LFAQVGDLTFNDVDELLSQYKQLVFKYISLSKGLSLTTSSVSVSHSEMQSHHRPESFKEP 420

Query: 421 EDVRDVTSNDESAGERHRLIDGSDGTSLFGEENVRSGLPQDKVVLPQHEGNEETSQS 475
           E +RDVTSN+ESAGE H+ IDG DG SLFGEE+V SGLPQDKVVL QHEGN++  QS
Sbjct: 421 EGIRDVTSNEESAGETHKRIDGPDGISLFGEESVGSGLPQDKVVLLQHEGNDKIPQS 476

BLAST of Bhi12G001197 vs. ExPASy TrEMBL
Match: A0A0A0LI50 (VPS9 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G128630 PE=4 SV=1)

HSP 1 Score: 850.5 bits (2196), Expect = 3.3e-243
Identity = 444/474 (93.67%), Postives = 453/474 (95.57%), Query Frame = 0

Query: 1   MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA
Sbjct: 1   MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQLSEKMALIQQFIR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQ+SEKMALIQQFIR
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQISEKMALIQQFIR 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN 180
           PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN
Sbjct: 121 PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN 180

Query: 181 LPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFILNI 240
            PGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTN+LSAESFI NI
Sbjct: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNVLSAESFISNI 240

Query: 241 DAKALSMEEIEFEKNMESAQALLSGLSSDVEALSNKSNLNEGVVHSSELVETSSQASVEP 300
           DAKALSMEEIEFEKNMESA+ALLSGLSSDVEALSNK+NL+EGV H SELVETSSQASVE 
Sbjct: 241 DAKALSMEEIEFEKNMESARALLSGLSSDVEALSNKNNLDEGVGHYSELVETSSQASVES 300

Query: 301 AVRPKSTEAKPRTEAPLAKDQSAVMEAPSLSDLENKGAAILLNDQTGGRQAFRDYPYLFS 360
            VRPK TE KPRTEAP AKDQSAVM+ PSLSDLENKGA ILLNDQTGGRQA R+YPYLFS
Sbjct: 301 PVRPKPTEGKPRTEAPHAKDQSAVMKVPSLSDLENKGATILLNDQTGGRQALREYPYLFS 360

Query: 361 QVGDLTFKDVEELLSQYKQLVFKYVSLSRGLSVTTAFSSYSNSEMHSQHHPESFKEPEDV 420
           QVGDLTFKDVEELLSQYKQLVFKYVSLSRGLS+TT FSSYS SEMHSQHH ESFKEPEDV
Sbjct: 361 QVGDLTFKDVEELLSQYKQLVFKYVSLSRGLSLTTEFSSYSKSEMHSQHHHESFKEPEDV 420

Query: 421 RDVTSNDESAGERHRLIDGSDGTSLFGEENVRSGLPQDKVVLPQHEGNEETSQS 475
           RDVTSNDESAG+RHRLIDG DGTSLFGEENV SGLPQDKV LPQ EGN E SQS
Sbjct: 421 RDVTSNDESAGDRHRLIDGPDGTSLFGEENVGSGLPQDKVELPQREGNNEMSQS 474

BLAST of Bhi12G001197 vs. ExPASy TrEMBL
Match: A0A1S3B4M4 (vacuolar protein sorting-associated protein 9A-like OS=Cucumis melo OX=3656 GN=LOC103485761 PE=4 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 1.2e-240
Identity = 443/474 (93.46%), Postives = 450/474 (94.94%), Query Frame = 0

Query: 1   MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA
Sbjct: 29  MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 88

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQLSEKMALIQQFIR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQLSEKMALIQQFIR
Sbjct: 89  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQLSEKMALIQQFIR 148

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN 180
           PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN
Sbjct: 149 PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN 208

Query: 181 LPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFILNI 240
            PGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFI NI
Sbjct: 209 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 268

Query: 241 DAKALSMEEIEFEKNMESAQALLSGLSSDVEALSNKSNLNEGVVHSSELVETSSQASVEP 300
           DAKALSMEEIEFEKNMESA+ALLSGLSSDVEA+SNK+NL+EGV H SELVETSSQASVE 
Sbjct: 269 DAKALSMEEIEFEKNMESARALLSGLSSDVEAISNKNNLDEGVGHYSELVETSSQASVES 328

Query: 301 AVRPKSTEAKPRTEAPLAKDQSAVMEAPSLSDLENKGAAILLNDQTGGRQAFRDYPYLFS 360
            VRPK TE KPRTEAP AKDQS VME PSLSDLENKGA ILLNDQTGGRQA RDYPYLFS
Sbjct: 329 QVRPKPTEGKPRTEAPHAKDQSTVMEVPSLSDLENKGATILLNDQTGGRQALRDYPYLFS 388

Query: 361 QVGDLTFKDVEELLSQYKQLVFKYVSLSRGLSVTTAFSSYSNSEMHSQHHPESFKEPEDV 420
           QVGDLTFKDVEELLSQYKQLVFKYVSLSRGL +TT  SSYSNSEM+SQHH ESFKEPEDV
Sbjct: 389 QVGDLTFKDVEELLSQYKQLVFKYVSLSRGLGLTTELSSYSNSEMYSQHHHESFKEPEDV 448

Query: 421 RDVTSNDESAGERHRLIDGSDGTSLFGEENVRSGLPQDKVVLPQHEGNEETSQS 475
           RD+TSNDESAGE HRLIDG DGTSLFGEENV SGL  DKVVLPQHEGN E SQS
Sbjct: 449 RDMTSNDESAGEGHRLIDGPDGTSLFGEENVGSGL--DKVVLPQHEGNNEMSQS 500

BLAST of Bhi12G001197 vs. ExPASy TrEMBL
Match: A0A5A7SSZ2 (Vacuolar protein sorting-associated protein 9A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold219G002250 PE=4 SV=1)

HSP 1 Score: 781.9 bits (2018), Expect = 1.5e-222
Identity = 413/444 (93.02%), Postives = 420/444 (94.59%), Query Frame = 0

Query: 31  SFIVSFSNNAPDPERDSASVQEFFAKMEGAFRAHPLWSGCSEEELESAGEGLEKYVMTKL 90
           SFIVSFSNNAPDPERDSASVQEFFAKMEGAFRAHPLWSGCSEEELESAGEGLEKYVMTKL
Sbjct: 137 SFIVSFSNNAPDPERDSASVQEFFAKMEGAFRAHPLWSGCSEEELESAGEGLEKYVMTKL 196

Query: 91  FSRVYASLADDVKIDEQLSEKMALIQQFIRPENLDIKPNFQNETSWLLAQKELLKINMHK 150
           FSRVYASLADDVKIDEQLSEKMALIQQFIRPENLDIKPNFQNETSWLLAQKELLKINMHK
Sbjct: 197 FSRVYASLADDVKIDEQLSEKMALIQQFIRPENLDIKPNFQNETSWLLAQKELLKINMHK 256

Query: 151 APRDKLVCLLSCCKVISNLLFNASIASNENLPGADEFLPVLIYVIIKANPPQLHSNLLYI 210
           APRDKLVCLLSCCKVISNLLFNASIASNEN PGADEFLPVLIYVIIKANPPQLHSNLLYI
Sbjct: 257 APRDKLVCLLSCCKVISNLLFNASIASNENPPGADEFLPVLIYVIIKANPPQLHSNLLYI 316

Query: 211 QRYRGQSRLTGEAAYFFTNILSAESFILNIDAKALSMEEIEFEKNMESAQALLSGLSSDV 270
           QRYRGQSRLTGEAAYFFTNILSAESFI NIDAKALSMEEIEFEKNMESA+ALLSGLSSDV
Sbjct: 317 QRYRGQSRLTGEAAYFFTNILSAESFISNIDAKALSMEEIEFEKNMESARALLSGLSSDV 376

Query: 271 EALSNKSNLNEGVVHSSELVETSSQASVEPAVRPKSTEAKPRTEAPLAKDQSAVMEAPSL 330
           EA+SNK+NL+EGV H SELVETSSQASVE  VRPK TE KPRTEAP AKDQS VME PSL
Sbjct: 377 EAISNKNNLDEGVGHYSELVETSSQASVESQVRPKPTEGKPRTEAPHAKDQSTVMEVPSL 436

Query: 331 SDLENKGAAILLNDQTGGRQAFRDYPYLFSQVGDLTFKDVEELLSQYKQLVFKYVSLSRG 390
           SDLENKGA ILLNDQTGGRQA RDYPYLFSQVGDLTFKDVEELLSQYKQLVFKYVSLSRG
Sbjct: 437 SDLENKGATILLNDQTGGRQALRDYPYLFSQVGDLTFKDVEELLSQYKQLVFKYVSLSRG 496

Query: 391 LSVTTAFSSYSNSEMHSQHHPESFKEPEDVRDVTSNDESAGERHRLIDGSDGTSLFGEEN 450
           L +TT  SSYSNSEM+SQHH ESFKEPEDVRD+TSNDESAGE HRLIDG DGTSLFGEEN
Sbjct: 497 LGLTTELSSYSNSEMYSQHHHESFKEPEDVRDMTSNDESAGEGHRLIDGPDGTSLFGEEN 556

Query: 451 VRSGLPQDKVVLPQHEGNEETSQS 475
           V SGL  DKVVLPQHEGN E SQS
Sbjct: 557 VGSGL--DKVVLPQHEGNNEMSQS 578

BLAST of Bhi12G001197 vs. ExPASy TrEMBL
Match: A0A6J1CKF0 (vacuolar protein sorting-associated protein 9A-like OS=Momordica charantia OX=3673 GN=LOC111011952 PE=4 SV=1)

HSP 1 Score: 779.6 bits (2012), Expect = 7.3e-222
Identity = 409/477 (85.74%), Postives = 438/477 (91.82%), Query Frame = 0

Query: 1   MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MEN DVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA
Sbjct: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQLSEKMALIQQFIR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKLF+RVYASL DDVKIDEQLS+K+ALIQQF R
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFTRVYASLRDDVKIDEQLSDKIALIQQFAR 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN 180
           PENLDIKPNFQNETSWLLAQKELLKINM+KAPRDKLVCLL+CCKVISNLL NAS+ASNEN
Sbjct: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASNEN 180

Query: 181 LPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFILNI 240
            PGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQ+RLTGEAAYFFTN+LSAESFI NI
Sbjct: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFISNI 240

Query: 241 DAKALSMEEIEFEKNMESAQALLSGLSSDVEALSNKSNLNEGVVHSSELVETSSQAS--- 300
           DAKALSM+EIEFE  MESA+ALLSGLS+D EA+SNKSNL+EGVV +S LV++SSQAS   
Sbjct: 241 DAKALSMDEIEFENKMESARALLSGLSTDAEAISNKSNLDEGVVRNSGLVKSSSQASNVG 300

Query: 301 VEPAVRPKSTEAKPRTEAPLAKDQSAVMEAPSLSDLENKGAAILLNDQTGGRQAFRDYPY 360
            EPAV PKS EAKPRTEA  AKDQS VME PSLSDLENKGAAILLND T GRQAFRDYPY
Sbjct: 301 TEPAVIPKSAEAKPRTEASCAKDQSTVMEEPSLSDLENKGAAILLNDHT-GRQAFRDYPY 360

Query: 361 LFSQVGDLTFKDVEELLSQYKQLVFKYVSLSRGLSVTTAFSSYSNSEMHSQHHPESFKEP 420
           LF+QVGDLTF DV+ELLSQYKQLVFKY+SLS+GLS+TT+  S S+SEM S H PESFKEP
Sbjct: 361 LFAQVGDLTFNDVDELLSQYKQLVFKYISLSKGLSLTTSSVSVSHSEMQSHHRPESFKEP 420

Query: 421 EDVRDVTSNDESAGERHRLIDGSDGTSLFGEENVRSGLPQDKVVLPQHEGNEETSQS 475
           E +RDVTSN+ESAGE H+ IDG DG SLFGEE+V SGLPQDKVVL QHEGN++  QS
Sbjct: 421 EGIRDVTSNEESAGETHKRIDGPDGISLFGEESVGSGLPQDKVVLLQHEGNDKIPQS 476

BLAST of Bhi12G001197 vs. ExPASy TrEMBL
Match: A0A6J1JJ31 (vacuolar protein sorting-associated protein 9A-like OS=Cucurbita maxima OX=3661 GN=LOC111487398 PE=4 SV=1)

HSP 1 Score: 771.9 bits (1992), Expect = 1.5e-219
Identity = 407/477 (85.32%), Postives = 434/477 (90.99%), Query Frame = 0

Query: 1   MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MEN DVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERD ASVQEFFAKMEGA
Sbjct: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDGASVQEFFAKMEGA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQLSEKMALIQQFIR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKL+SRVYASLADDVKIDEQLS+KMALIQQFIR
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN 180
           PENLDIKPNFQNE+SWLLAQKELLKINM+KAPRDKLVCLL CCKVISNLL NASIASNEN
Sbjct: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180

Query: 181 LPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFILNI 240
            PGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFI NI
Sbjct: 181 SPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240

Query: 241 DAKALSMEEIEFEKNMESAQALLSGLSSDVEALSNKSNLNEGVVHSSELVETSSQAS--- 300
           DA +LSMEE EFEKNMESA+ALLSGLS+DVE+ SNKSNL+E V  +SELVETSSQAS   
Sbjct: 241 DANSLSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300

Query: 301 VEPAVRPKSTEAKPRTEAPLAKDQSAVMEAPSLSDLENKGAAILLNDQTGGRQAFRDYPY 360
            EPAVRP S E+KP TE P  K QS VME  +LSDLE+KGAA+LLND T  +QAFRDYPY
Sbjct: 301 TEPAVRPNSPESKPSTEGPHVKYQSTVMEVSTLSDLESKGAAMLLNDHT-RKQAFRDYPY 360

Query: 361 LFSQVGDLTFKDVEELLSQYKQLVFKYVSLSRGLSVTTAFSSYSNSEMHSQHHPESFKEP 420
           LF+QVGDLTF DVEELL+QY+ +VFKYVSLS+GLS+ ++  S S+SEMHS H+PESFKEP
Sbjct: 361 LFAQVGDLTFNDVEELLNQYQHVVFKYVSLSKGLSLRSSSPSSSSSEMHSHHNPESFKEP 420

Query: 421 EDVRDVTSNDESAGERHRLIDGSDGTSLFGEENVRSGLPQDKVVLPQHEGNEETSQS 475
           EDVRDVTSNDESAGE H +IDG DGTSLFGEENV SGL QDKVVLPQHEGN+ TS S
Sbjct: 421 EDVRDVTSNDESAGETHTMIDGPDGTSLFGEENVGSGLMQDKVVLPQHEGNDTTSLS 476

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G19770.11.1e-14262.41Vacuolar sorting protein 9 (VPS9) domain [more]
AT5G09320.11.4e-12957.34Vacuolar sorting protein 9 (VPS9) domain [more]
AT3G19770.21.9e-7353.47Vacuolar sorting protein 9 (VPS9) domain [more]
Match NameE-valueIdentityDescription
Q9LT311.6e-14162.41Vacuolar protein sorting-associated protein 9A OS=Arabidopsis thaliana OX=3702 G... [more]
Q10NQ33.4e-12858.47Vacuolar protein sorting-associated protein 9A OS=Oryza sativa subsp. japonica O... [more]
O743963.9e-3932.58Vacuolar protein sorting-associated protein 9a OS=Schizosaccharomyces pombe (str... [more]
A5D7945.3e-2834.86GTPase-activating protein and VPS9 domain-containing protein 1 OS=Bos taurus OX=... [more]
Q14C865.3e-2834.86GTPase-activating protein and VPS9 domain-containing protein 1 OS=Homo sapiens O... [more]
Match NameE-valueIdentityDescription
XP_038874663.17.3e-261100.00vacuolar protein sorting-associated protein 9A-like [Benincasa hispida] >XP_0388... [more]
XP_004138949.26.9e-24393.67vacuolar protein sorting-associated protein 9A isoform X1 [Cucumis sativus][more]
XP_008441683.22.5e-24093.46PREDICTED: vacuolar protein sorting-associated protein 9A-like [Cucumis melo][more]
KAA0032359.13.0e-22293.02vacuolar protein sorting-associated protein 9A-like [Cucumis melo var. makuwa][more]
XP_022141647.11.5e-22185.74vacuolar protein sorting-associated protein 9A-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A0A0LI503.3e-24393.67VPS9 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G128630 PE=4 S... [more]
A0A1S3B4M41.2e-24093.46vacuolar protein sorting-associated protein 9A-like OS=Cucumis melo OX=3656 GN=L... [more]
A0A5A7SSZ21.5e-22293.02Vacuolar protein sorting-associated protein 9A-like OS=Cucumis melo var. makuwa ... [more]
A0A6J1CKF07.3e-22285.74vacuolar protein sorting-associated protein 9A-like OS=Momordica charantia OX=36... [more]
A0A6J1JJ311.5e-21985.32vacuolar protein sorting-associated protein 9A-like OS=Cucurbita maxima OX=3661 ... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 256..276
NoneNo IPR availableGENE3D1.10.246.120coord: 17..97
e-value: 5.2E-29
score: 102.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 408..439
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 403..474
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 290..323
NoneNo IPR availablePANTHERPTHR23101:SF110BNAC09G47180D PROTEINcoord: 1..408
IPR003123VPS9 domainSMARTSM00167vps9_2coord: 135..254
e-value: 1.1E-26
score: 104.7
IPR003123VPS9 domainPFAMPF02204VPS9coord: 138..241
e-value: 5.6E-28
score: 97.2
IPR003123VPS9 domainPROSITEPS51205VPS9coord: 102..246
score: 41.900139
IPR037191VPS9 domain superfamilyGENE3D1.20.1050.80VPS9 domaincoord: 98..265
e-value: 6.6E-65
score: 219.9
IPR037191VPS9 domain superfamilySUPERFAMILY109993VPS9 domaincoord: 11..257
IPR041545RABX5, catalytic core helical domainPFAMPF18151DUF5601coord: 27..91
e-value: 7.6E-17
score: 61.3
IPR045046Vacuolar protein sorting-associated protein 9-likePANTHERPTHR23101RAB GDP/GTP EXCHANGE FACTORcoord: 1..408

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi12M001197Bhi12M001197mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006886 intracellular protein transport
biological_process GO:0050790 regulation of catalytic activity
biological_process GO:0016192 vesicle-mediated transport
cellular_component GO:0000325 plant-type vacuole
cellular_component GO:0005802 trans-Golgi network
molecular_function GO:0005085 guanyl-nucleotide exchange factor activity