Bhi12G000967 (gene) Wax gourd (B227) v1

Overview
NameBhi12G000967
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
Locationchr12: 33400608 .. 33402959 (-)
RNA-Seq ExpressionBhi12G000967
SyntenyBhi12G000967
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACCGACAAACAAACACACATTTTTATTTTTATCACTTTCATTTCTCAGAAAAAACACATTCTCAAACTGAAGAAGAAGAAGAAGAAGAAGAAGAAAAAAGAAGAAAAAAACAGAGCATTAATGGCCACTCTTCAATCATCTTCTCTCATTACCCAAAGGTAATTTCCATATCCTTTCCTTATCTTTCTTCTTCTTTTTGTCTCTTTTGTTTGATTCATCTTTCTGGGTTTTCAAATCTGAACCTCTCTTATTATGGATCTGCCCTTTTCTACAAACCCAACCTTTAAAAGAGCTTCCTTTCTTTTGTTTTTGTTTCTTTAATTTCTGACCCTTTTTGGATTTTGAAGCTTTGATGTCAAATCCTTTTGATTTGCTTTTCAATTTCATTTCTTAGTTCCAAAAAGGGTTGATTTACTCTGTTGTTGATACAACTCAGTGCTTGTTTTTTATAGGTTTATTTATAGATTTTATTTGGTTCATGGTTGCTCTTTTTAAGTAATTTTGGTTCTTTTAGAGTAGATAATCATGTTTTAAAAGACAATTATTTAAAAAAAAAAAAAATTGATTATAGGATGTTCCTGGAATGTTTTGAATCATGGCAGTGGGAGAAATTAACTAAAAATAGAATCTTTATCAAACTAACTTTTCAAATATTCTGTTGAAATTTAATTTTCTAACATTTTTCTTAATTTGTATGACTACTGAACATTTGTGAATAACAATCCAAGAAAGCAAAAGAAATATCATATAATTAAATTTTCCATAACCATAAACTTAAGTTGATTACACTAGGAGATGATCATTGTGTTCAAACTTGTGTCATGTCATTAGTATTAGTTTTTCGGTTCAATAGTGAGTTGGCGATATGAAAATTGATTTCTGTTCTTAAAACTTGCTTAGTTTGTTAATTGTACCAAATAAAATGAACTTCTAGCTGTTTGCTGGATTTGGAACCCCCAATTGTTTTGCTAGCTTATGCTCATTCCACACCTGCAAAAGCAGAACTAGACGAAGTATTTTAACACTACGGTTGGCTTTCGAATCCCGAATGGCGAACGGAGTCGATTGTATCGTATAAAATGACGAAAAGAAAAGAAAAGAATGGTCGCTTTACTGTAAATTTTTTTACCAAGATCAGTTTGCACCATGATAAAGGTCTTGATTGAAGGATAAACAAAGGATTTCTCAGCAAATTCTTGATGGAAATGAGGTCGCTATGCGTTGTTACATTCGATTGTCAGTTAGCTTTTCATTCGTTATGATCGTAACCATAGATAATCTCTCTTTAGATTTGGTATAGGAATGTGGAAGTGTTTGAATACGAACTCTTGATTGATTCTTCCCATTTTCAGGTTATTAGGGAAAGTGGCATTAGTCACTGGTGGAGCCTCTGGCATTGGAGAGGCCATTGTGCGCCTCTTCCACAGACATGGTGCAAAGGTTTGTTTCGTCGATGTGCAGGACGAGCCCGGTTATCGTCTCCATGAAAACCTCGGTGGCGACAAAGACTCGAACACTTATTATTCTCATTGTGATGTCACTGTGGAAGAGGATATTCGTCGTGCAGTCGACTTGACAGTAAGTAAATTCGGTACACTTGACATAATGGTGAACAATGCTGGGATCTCGGGCACCCCCTCGTCCGATATTCGCAATGTCGATGTATCGGAGTTCGAGAGGGTGTTTGATATAAATGTGAAGGCAGTTTTCATGGGAATGAAGTATGCTGCTAGTGTAATGATTCCGCGCAAGCAAGGATCGATCATATCACTCGGCAGCGTTGGTAGCGTCATAGGAGGAATAGGGCCCCATCATTACGTAGGTTCGAAGCATGCCGTATTGGGGCTTACGAGGAGTGTTGCAGCCGAGCTTGGACAACATGGAATTCGTGTAAATTGTGTCTCTCCTTACGGGGTTCCAACGGGATTGTCTGTAGCTCACTTGCCTGAAGAGGAGAGAACTGAGGATGTGTTTACAGATTTTCGTGAGTTTGCTAAAAAGAATGCTAATTTGCAGGGAGTAGAACTTGAGGTTGAAGATGTTGCCAATGCTGTCTTGTTCTTGGCTAGCGACGAGGCTCGATACATCAGCGGAGACAATCTCCTTGTTGATGGTGGCTTCACTCGTGTAAATCACTCGCTTCGTGTTTTCAAATGATCAACGTAGTCTTTAGAAGGAATGAGTTTATATCGTATATGGTGACTACAAACCTAAGATTTATGATTCTATATGTTATTTAATAAAAATATCCATTATGTTTATATATTGAATGTTATAAGCTTATATTAATATTTTTAACACATTCTTTGGTATACTTTTCTCTATATATATTTTATTTTCCTTATTTGTC

mRNA sequence

AAACCGACAAACAAACACACATTTTTATTTTTATCACTTTCATTTCTCAGAAAAAACACATTCTCAAACTGAAGAAGAAGAAGAAGAAGAAGAAGAAAAAAGAAGAAAAAAACAGAGCATTAATGGCCACTCTTCAATCATCTTCTCTCATTACCCAAAGGTTATTAGGGAAAGTGGCATTAGTCACTGGTGGAGCCTCTGGCATTGGAGAGGCCATTGTGCGCCTCTTCCACAGACATGGTGCAAAGGTTTGTTTCGTCGATGTGCAGGACGAGCCCGGTTATCGTCTCCATGAAAACCTCGGTGGCGACAAAGACTCGAACACTTATTATTCTCATTGTGATGTCACTGTGGAAGAGGATATTCGTCGTGCAGTCGACTTGACAGTAAGTAAATTCGGTACACTTGACATAATGGTGAACAATGCTGGGATCTCGGGCACCCCCTCGTCCGATATTCGCAATGTCGATGTATCGGAGTTCGAGAGGGTGTTTGATATAAATGTGAAGGCAGTTTTCATGGGAATGAAGTATGCTGCTAGTGTAATGATTCCGCGCAAGCAAGGATCGATCATATCACTCGGCAGCGTTGGTAGCGTCATAGGAGGAATAGGGCCCCATCATTACGTAGGTTCGAAGCATGCCGTATTGGGGCTTACGAGGAGTGTTGCAGCCGAGCTTGGACAACATGGAATTCGTGTAAATTGTGTCTCTCCTTACGGGGTTCCAACGGGATTGTCTGTAGCTCACTTGCCTGAAGAGGAGAGAACTGAGGATGTGTTTACAGATTTTCGTGAGTTTGCTAAAAAGAATGCTAATTTGCAGGGAGTAGAACTTGAGGTTGAAGATGTTGCCAATGCTGTCTTGTTCTTGGCTAGCGACGAGGCTCGATACATCAGCGGAGACAATCTCCTTGTTGATGGTGGCTTCACTCGTGTAAATCACTCGCTTCGTGTTTTCAAATGATCAACGTAGTCTTTAGAAGGAATGAGTTTATATCGTATATGGTGACTACAAACCTAAGATTTATGATTCTATATGTTATTTAATAAAAATATCCATTATGTTTATATATTGAATGTTATAAGCTTATATTAATATTTTTAACACATTCTTTGGTATACTTTTCTCTATATATATTTTATTTTCCTTATTTGTC

Coding sequence (CDS)

ATGGCCACTCTTCAATCATCTTCTCTCATTACCCAAAGGTTATTAGGGAAAGTGGCATTAGTCACTGGTGGAGCCTCTGGCATTGGAGAGGCCATTGTGCGCCTCTTCCACAGACATGGTGCAAAGGTTTGTTTCGTCGATGTGCAGGACGAGCCCGGTTATCGTCTCCATGAAAACCTCGGTGGCGACAAAGACTCGAACACTTATTATTCTCATTGTGATGTCACTGTGGAAGAGGATATTCGTCGTGCAGTCGACTTGACAGTAAGTAAATTCGGTACACTTGACATAATGGTGAACAATGCTGGGATCTCGGGCACCCCCTCGTCCGATATTCGCAATGTCGATGTATCGGAGTTCGAGAGGGTGTTTGATATAAATGTGAAGGCAGTTTTCATGGGAATGAAGTATGCTGCTAGTGTAATGATTCCGCGCAAGCAAGGATCGATCATATCACTCGGCAGCGTTGGTAGCGTCATAGGAGGAATAGGGCCCCATCATTACGTAGGTTCGAAGCATGCCGTATTGGGGCTTACGAGGAGTGTTGCAGCCGAGCTTGGACAACATGGAATTCGTGTAAATTGTGTCTCTCCTTACGGGGTTCCAACGGGATTGTCTGTAGCTCACTTGCCTGAAGAGGAGAGAACTGAGGATGTGTTTACAGATTTTCGTGAGTTTGCTAAAAAGAATGCTAATTTGCAGGGAGTAGAACTTGAGGTTGAAGATGTTGCCAATGCTGTCTTGTTCTTGGCTAGCGACGAGGCTCGATACATCAGCGGAGACAATCTCCTTGTTGATGGTGGCTTCACTCGTGTAAATCACTCGCTTCGTGTTTTCAAATGA

Protein sequence

MATLQSSSLITQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDEPGYRLHENLGGDKDSNTYYSHCDVTVEEDIRRAVDLTVSKFGTLDIMVNNAGISGTPSSDIRNVDVSEFERVFDINVKAVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVLGLTRSVAAELGQHGIRVNCVSPYGVPTGLSVAHLPEEERTEDVFTDFREFAKKNANLQGVELEVEDVANAVLFLASDEARYISGDNLLVDGGFTRVNHSLRVFK
Homology
BLAST of Bhi12G000967 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 384.0 bits (985), Expect = 1.0e-106
Identity = 189/275 (68.73%), Postives = 224/275 (81.45%), Query Frame = 0

Query: 7   SSLITQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDEPGYRLHEN-LGGDKD 66
           SSL +QRLLGKVAL+TGGA+GIGE+IVRLFH+HGAKVC VD+QD+ G  + ++ L G+  
Sbjct: 11  SSLPSQRLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEVCKSLLRGESK 70

Query: 67  SNTYYSHCDVTVEEDIRRAVDLTVSKFGTLDIMVNNAGISGTPSSDIRNVDVSEFERVFD 126
              ++ H DV VE+DI  AVD  V  FGTLDI++NNAG+ G P  DIRN  +SEFE  FD
Sbjct: 71  ETAFFIHGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCGAPCPDIRNYSLSEFEMTFD 130

Query: 127 INVKAVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVLGLTRSVAAE 186
           +NVK  F+ MK+AA VMIP K+GSI+SL SVG V+GG+GPH YVGSKHAVLGLTRSVAAE
Sbjct: 131 VNVKGAFLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSKHAVLGLTRSVAAE 190

Query: 187 LGQHGIRVNCVSPYGVPTGLSVAHLPEEERTEDVFTDFREFAKKNANLQGVELEVEDVAN 246
           LGQHGIRVNCVSPY V T L++AHLPEEERTED F  FR FA  NANL+GVEL V+DVAN
Sbjct: 191 LGQHGIRVNCVSPYAVATKLALAHLPEEERTEDAFVGFRNFAAANANLKGVELTVDDVAN 250

Query: 247 AVLFLASDEARYISGDNLLVDGGFTRVNHSLRVFK 281
           AVLFLASD++RYISGDNL++DGGFT  NHS +VF+
Sbjct: 251 AVLFLASDDSRYISGDNLMIDGGFTCTNHSFKVFR 285

BLAST of Bhi12G000967 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 232.6 bits (592), Expect = 3.8e-61
Identity = 125/272 (45.96%), Postives = 175/272 (64.34%), Query Frame = 0

Query: 6   SSSLITQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDEPGYRLHENLGGDKD 65
           +++L  +RL GKVA++TGGA GIG+A V LF RHGA V   DV +  G  L ++L   K 
Sbjct: 24  NTTLYPKRLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKT 83

Query: 66  S-NTYYSHCDVTVEEDIRRAVDLTVSKFGTLDIMVNNAGISGTPS--SDIRNVDVSEFER 125
           S    +  CDV+VE D+   V++TV+++G LDI+ NNAG+ G       I + D  EF+ 
Sbjct: 84  SPMVAFISCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDH 143

Query: 126 VFDINVKAVFMGMKYAASVMIPRK-QGSIISLGSVGSVIGGIGPHHYVGSKHAVLGLTRS 185
           V  +NV+ V +GMK+ A  MI R  +G IIS  SV  V+GG+GPH Y  SKHA++GLT++
Sbjct: 144 VMRVNVRGVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKN 203

Query: 186 VAAELGQHGIRVNCVSPYGVPTGLSV---AHLPEEERTEDVFTDFREFAKKNANLQGVEL 245
            A ELG++GIRVNC+SP+GV T + V         +  +D   +  EF +  ANL+G  L
Sbjct: 204 AACELGKYGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLKGETL 263

Query: 246 EVEDVANAVLFLASDEARYISGDNLLVDGGFT 271
              D+A A L+LASDE++Y++G NL+VDGG T
Sbjct: 264 RANDIAEAALYLASDESKYVNGHNLVVDGGVT 295

BLAST of Bhi12G000967 vs. TAIR 10
Match: AT3G26770.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 219.5 bits (558), Expect = 3.3e-57
Identity = 121/264 (45.83%), Postives = 171/264 (64.77%), Query Frame = 0

Query: 11  TQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDEPGYRLHENLGGDKDSNTYY 70
           +++L GKVAL+TGGASG+G+A    F RHGA+V   D+  E G +  + LG + +    +
Sbjct: 38  SKKLEGKVALITGGASGLGKATASEFLRHGARVVIADLDAETGTKTAKELGSEAE----F 97

Query: 71  SHCDVTVEEDIRRAVDLTVSKFGTLDIMVNNAGISG--TPSSDIRNVDVSEFERVFDINV 130
             CDVTVE DI  AV++TV ++G LD+M NNAGI G  TP+S I  +D++EFERV  INV
Sbjct: 98  VRCDVTVEADIAGAVEMTVERYGKLDVMYNNAGIVGPMTPAS-ISQLDMTEFERVMRINV 157

Query: 131 KAVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVLGLTRSVAAELGQ 190
             V  G+K+AA  MIP + G I+   SV  V GG+ PH Y  SK    G+ +S A+EL +
Sbjct: 158 FGVVSGIKHAAKFMIPARSGCILCTSSVAGVTGGLAPHSYTISKFTTPGIVKSAASELCE 217

Query: 191 HGIRVNCVSPYGVPTGLSVAHLPE--EERTEDVFTDFREFAKKNANLQGVELEVEDVANA 250
           HG+R+NC+SP  V T L++++L +   + +E+     RE  K    L+G E E  DVA A
Sbjct: 218 HGVRINCISPGTVATPLTLSYLQKVFPKVSEE---KLRETVKGMGELKGAECEEADVAKA 277

Query: 251 VLFLASDEARYISGDNLLVDGGFT 271
            L+LAS++ +Y++G NL+VDGG T
Sbjct: 278 ALYLASNDGKYVTGHNLVVDGGMT 293

BLAST of Bhi12G000967 vs. TAIR 10
Match: AT3G29250.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 216.9 bits (551), Expect = 2.1e-56
Identity = 122/261 (46.74%), Postives = 166/261 (63.60%), Query Frame = 0

Query: 13  RLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDEPGYRLHENLGGDKDSNTYYSH 72
           RL GK+A++TGGASGIG   VRLF  HGAKV  VD+Q+E G  L  ++G DK S   +  
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLDKAS---FYR 64

Query: 73  CDVTVEEDIRRAVDLTVSKFGTLDIMVNNAGISGTPSSDIRNVDVSEFERVFDINVKAVF 132
           C+VT E D+  AV  TV K G LD++ +NAG+     S + ++D+  F+R   +NV+   
Sbjct: 65  CNVTDETDVENAVKFTVEKHGKLDVLFSNAGVLEAFGS-VLDLDLEAFDRTMAVNVRGAA 124

Query: 133 MGMKYAASVMIPR-KQGSIISLGSVGSVIGGIGPHHYVGSKHAVLGLTRSVAAELGQHGI 192
             +K+AA  M+    +GSI+   S+ + IGG GPH Y  SKHA+LGL RS  A LGQ+GI
Sbjct: 125 AFIKHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGI 184

Query: 193 RVNCVSPYGVPTGLSVAHLPEEERTEDVFTDFREFAKKNANLQGVELEVEDVANAVLFLA 252
           RVN V+PYGV TG++ A+       E+      E+ +   NL+GV L+   +A A LFLA
Sbjct: 185 RVNGVAPYGVATGMTSAY------NEEAVKMLEEYGEALGNLKGVVLKARHIAEAALFLA 244

Query: 253 SDEARYISGDNLLVDGGFTRV 273
           SD++ YISG NL+VDGGF+ V
Sbjct: 245 SDDSVYISGQNLVVDGGFSVV 255

BLAST of Bhi12G000967 vs. TAIR 10
Match: AT3G29250.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 216.5 bits (550), Expect = 2.8e-56
Identity = 123/269 (45.72%), Postives = 170/269 (63.20%), Query Frame = 0

Query: 5   QSSSLITQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDEPGYRLHENLGGDK 64
           ++ S + Q L GK+A++TGGASGIG   VRLF  HGAKV  VD+Q+E G  L  ++G DK
Sbjct: 35  KTMSGLRQVLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLDK 94

Query: 65  DSNTYYSHCDVTVEEDIRRAVDLTVSKFGTLDIMVNNAGISGTPSSDIRNVDVSEFERVF 124
            S   +  C+VT E D+  AV  TV K G LD++ +NAG+     S + ++D+  F+R  
Sbjct: 95  AS---FYRCNVTDETDVENAVKFTVEKHGKLDVLFSNAGVLEAFGS-VLDLDLEAFDRTM 154

Query: 125 DINVKAVFMGMKYAASVMIPR-KQGSIISLGSVGSVIGGIGPHHYVGSKHAVLGLTRSVA 184
            +NV+     +K+AA  M+    +GSI+   S+ + IGG GPH Y  SKHA+LGL RS  
Sbjct: 155 AVNVRGAAAFIKHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSAC 214

Query: 185 AELGQHGIRVNCVSPYGVPTGLSVAHLPEEERTEDVFTDFREFAKKNANLQGVELEVEDV 244
           A LGQ+GIRVN V+PYGV TG++ A+       E+      E+ +   NL+GV L+   +
Sbjct: 215 AGLGQYGIRVNGVAPYGVATGMTSAY------NEEAVKMLEEYGEALGNLKGVVLKARHI 274

Query: 245 ANAVLFLASDEARYISGDNLLVDGGFTRV 273
           A A LFLASD++ YISG NL+VDGGF+ V
Sbjct: 275 AEAALFLASDDSVYISGQNLVVDGGFSVV 293

BLAST of Bhi12G000967 vs. ExPASy Swiss-Prot
Match: Q9C826 (Xanthoxin dehydrogenase OS=Arabidopsis thaliana OX=3702 GN=ABA2 PE=1 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 1.4e-105
Identity = 189/275 (68.73%), Postives = 224/275 (81.45%), Query Frame = 0

Query: 7   SSLITQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDEPGYRLHEN-LGGDKD 66
           SSL +QRLLGKVAL+TGGA+GIGE+IVRLFH+HGAKVC VD+QD+ G  + ++ L G+  
Sbjct: 11  SSLPSQRLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEVCKSLLRGESK 70

Query: 67  SNTYYSHCDVTVEEDIRRAVDLTVSKFGTLDIMVNNAGISGTPSSDIRNVDVSEFERVFD 126
              ++ H DV VE+DI  AVD  V  FGTLDI++NNAG+ G P  DIRN  +SEFE  FD
Sbjct: 71  ETAFFIHGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCGAPCPDIRNYSLSEFEMTFD 130

Query: 127 INVKAVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVLGLTRSVAAE 186
           +NVK  F+ MK+AA VMIP K+GSI+SL SVG V+GG+GPH YVGSKHAVLGLTRSVAAE
Sbjct: 131 VNVKGAFLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSKHAVLGLTRSVAAE 190

Query: 187 LGQHGIRVNCVSPYGVPTGLSVAHLPEEERTEDVFTDFREFAKKNANLQGVELEVEDVAN 246
           LGQHGIRVNCVSPY V T L++AHLPEEERTED F  FR FA  NANL+GVEL V+DVAN
Sbjct: 191 LGQHGIRVNCVSPYAVATKLALAHLPEEERTEDAFVGFRNFAAANANLKGVELTVDDVAN 250

Query: 247 AVLFLASDEARYISGDNLLVDGGFTRVNHSLRVFK 281
           AVLFLASD++RYISGDNL++DGGFT  NHS +VF+
Sbjct: 251 AVLFLASDDSRYISGDNLMIDGGFTCTNHSFKVFR 285

BLAST of Bhi12G000967 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 2.3e-95
Identity = 171/268 (63.81%), Postives = 213/268 (79.48%), Query Frame = 0

Query: 13  RLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDEPGYRLHENLGGDKDSNTYYSH 72
           RL GKVALVTGGASGIGE+I RLF  HGAK+C VDVQDE G ++ + LGG  D +  Y H
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRLGG--DPHACYFH 61

Query: 73  CDVTVEEDIRRAVDLTVSKFGTLDIMVNNAGISGTPSSDIRNVDVSEFERVFDINVKAVF 132
           CDVTVE+D+RRAVD T  K+GT+DIMVNNAGI+G    DIR+ D +EF++VFDINV  VF
Sbjct: 62  CDVTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGDKVIDIRDADFNEFKKVFDINVNGVF 121

Query: 133 MGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVLGLTRSVAAELGQHGIR 192
           +GMK+AA +MIP+ +GSI+SL SV SVI G GPH Y G+KHAV+GLT+SVAAELG+HGIR
Sbjct: 122 LGMKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGIR 181

Query: 193 VNCVSPYGVPTGLSVAHLPEEERTEDVFTDFREFAKKNANLQGVELEVEDVANAVLFLAS 252
           VNCVSPY VPT LS+ +LPE E  ED    F  F + NANL+GV+L   DVA AVL+LA+
Sbjct: 182 VNCVSPYAVPTRLSMPYLPESEMQEDALRGFLTFVRSNANLKGVDLMPNDVAEAVLYLAT 241

Query: 253 DEARYISGDNLLVDGGFTRVNHSLRVFK 281
           +E++Y+SG NL++DGGF+  NH+L+VF+
Sbjct: 242 EESKYVSGLNLVIDGGFSIANHTLQVFE 267

BLAST of Bhi12G000967 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 9.3e-73
Identity = 151/281 (53.74%), Postives = 194/281 (69.04%), Query Frame = 0

Query: 2   ATLQSSSLITQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDEPGYRLHENLG 61
           AT Q  + I +RL GKVAL+TGGASGIGE   +LF +HGAKV   DVQDE G+ + E +G
Sbjct: 3   ATSQVLTAIARRLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIG 62

Query: 62  GDKDSNTYYSHCDVTVEEDIRRAVDLTVSKFGTLDIMVNNAGISGTPSSDIRNVDVSEFE 121
               SN+ Y HCDVT E+ ++ AVD TVS +G LDIM +NAGIS      I + + ++FE
Sbjct: 63  ---TSNSTYIHCDVTNEDGVKNAVDNTVSTYGKLDIMFSNAGISDPNRPRIIDNEKADFE 122

Query: 122 RVFDINVKAVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVLGLTRS 181
           RVF +NV  VF+ MK+AA VMIP + G+IIS  S+ S +GG   H Y GSKHAVLGLTR+
Sbjct: 123 RVFSVNVTGVFLCMKHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRN 182

Query: 182 VAAELGQHGIRVNCVSPYGVPT--GLSVAHLPEEERTEDVFTDFREFAKKNANLQGVELE 241
           +A ELGQ GIRVNC+SP+G+PT  G   + +  EE  E+V      FA    NL+G +  
Sbjct: 183 LAVELGQFGIRVNCLSPFGLPTALGKKFSGIKNEEEFENVI----NFA---GNLKGPKFN 242

Query: 242 VEDVANAVLFLASDEARYISGDNLLVDGGFTRVNHSLRVFK 281
           VEDVANA L+LASDEA+Y+SG NL +DGGF+  N  ++VF+
Sbjct: 243 VEDVANAALYLASDEAKYVSGHNLFIDGGFSVCNSVIKVFQ 273

BLAST of Bhi12G000967 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 8.7e-63
Identity = 132/271 (48.71%), Postives = 174/271 (64.21%), Query Frame = 0

Query: 12  QRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDEPGYRLHENLGGDKDSNTYYS 71
           ++L+GKVA++TGGASGIG    RLF +HGA+V   D+QDE G  L   LG D  S   Y 
Sbjct: 13  RKLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLVAELGPDASS---YV 72

Query: 72  HCDVTVEEDIRRAVDLTVSKFGTLDIMVNNAGISGTPSSDIRNVDVSEFERVFDINVKAV 131
           HCDVT E D+  AVD  V++FG LD+M NNAG+SG P   +      +FERV  +N+   
Sbjct: 73  HCDVTNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMSECTKEDFERVLAVNLVGP 132

Query: 132 FMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVLGLTRSVAAELGQHGI 191
           F+G K+AA VM P ++GSIIS  S+ S + G   H Y  SKHA++G T + A ELG+HGI
Sbjct: 133 FLGTKHAARVMAPARRGSIISTASLSSSVSGAASHAYTTSKHALVGFTENAAGELGRHGI 192

Query: 192 RVNCVSPYGVPTGLSVAHL-PEEERTEDVFTDFREFAKKNANLQGV-ELEVEDVANAVLF 251
           RVNCVSP GV T L+ A +  ++E  E +  +       +ANL+G   L+ +D+A A LF
Sbjct: 193 RVNCVSPAGVATPLARAAMGMDDEAIEAIMAN-------SANLKGAGALKADDIAAAALF 252

Query: 252 LASDEARYISGDNLLVDGGFTRVNHSLRVFK 281
           LASD+ RY+SG NL VDGG + VN S   F+
Sbjct: 253 LASDDGRYVSGQNLRVDGGLSVVNSSFGFFR 273

BLAST of Bhi12G000967 vs. ExPASy Swiss-Prot
Match: P50160 (Sex determination protein tasselseed-2 OS=Zea mays OX=4577 GN=TS2 PE=2 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 1.6e-61
Identity = 133/282 (47.16%), Postives = 174/282 (61.70%), Query Frame = 0

Query: 12  QRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDEPGYRLHENLGGDKDSNTYYS 71
           +RL GKVA+VTGGA GIGEAIVRLF +HGA+V   D+ D  G  L   LG        + 
Sbjct: 51  KRLDGKVAIVTGGARGIGEAIVRLFAKHGARVVIADIDDAAGEALASALG----PQVSFV 110

Query: 72  HCDVTVEEDIRRAVDLTVSKF-GTLDIMVNNAGISGTPSSDIRNV---DVSEFERVFDIN 131
            CDV+VE+D+RRAVD  +S+  G LD+  NNAG+ G  +   R++   D +EF+RV  +N
Sbjct: 111 RCDVSVEDDVRRAVDWALSRHGGRLDVYCNNAGVLGRQTRAARSILSFDAAEFDRVLRVN 170

Query: 132 VKAVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVLGLTRSVAAELG 191
                +GMK+AA  M PR+ GSI+S+ SV +V+GG+GPH Y  SKHA++GLT++ A EL 
Sbjct: 171 ALGAALGMKHAARAMAPRRAGSIVSVASVAAVLGGLGPHAYTASKHAIVGLTKNAACELR 230

Query: 192 QHGIRVNCVSPYGVPTGLSVA--HLPEEERTEDVFTD-----------------FREFAK 251
            HG+RVNCVSP+GV T + +       ++ T D   D                   E  +
Sbjct: 231 AHGVRVNCVSPFGVATPMLINAWRQGHDDATADADRDLDLDLDVTVPSDQEVEKMEEVVR 290

Query: 252 KNANLQGVELEVEDVANAVLFLASDEARYISGDNLLVDGGFT 271
             A L+G  L   D+A AVLFLASDEARYISG NL+VDGG T
Sbjct: 291 GLATLKGPTLRPRDIAEAVLFLASDEARYISGHNLVVDGGVT 328

BLAST of Bhi12G000967 vs. NCBI nr
Match: XP_038906598.1 (xanthoxin dehydrogenase [Benincasa hispida])

HSP 1 Score: 553.1 bits (1424), Expect = 1.3e-153
Identity = 280/280 (100.00%), Postives = 280/280 (100.00%), Query Frame = 0

Query: 1   MATLQSSSLITQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDEPGYRLHENL 60
           MATLQSSSLITQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDEPGYRLHENL
Sbjct: 1   MATLQSSSLITQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDEPGYRLHENL 60

Query: 61  GGDKDSNTYYSHCDVTVEEDIRRAVDLTVSKFGTLDIMVNNAGISGTPSSDIRNVDVSEF 120
           GGDKDSNTYYSHCDVTVEEDIRRAVDLTVSKFGTLDIMVNNAGISGTPSSDIRNVDVSEF
Sbjct: 61  GGDKDSNTYYSHCDVTVEEDIRRAVDLTVSKFGTLDIMVNNAGISGTPSSDIRNVDVSEF 120

Query: 121 ERVFDINVKAVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVLGLTR 180
           ERVFDINVKAVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVLGLTR
Sbjct: 121 ERVFDINVKAVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVLGLTR 180

Query: 181 SVAAELGQHGIRVNCVSPYGVPTGLSVAHLPEEERTEDVFTDFREFAKKNANLQGVELEV 240
           SVAAELGQHGIRVNCVSPYGVPTGLSVAHLPEEERTEDVFTDFREFAKKNANLQGVELEV
Sbjct: 181 SVAAELGQHGIRVNCVSPYGVPTGLSVAHLPEEERTEDVFTDFREFAKKNANLQGVELEV 240

Query: 241 EDVANAVLFLASDEARYISGDNLLVDGGFTRVNHSLRVFK 281
           EDVANAVLFLASDEARYISGDNLLVDGGFTRVNHSLRVFK
Sbjct: 241 EDVANAVLFLASDEARYISGDNLLVDGGFTRVNHSLRVFK 280

BLAST of Bhi12G000967 vs. NCBI nr
Match: XP_008465699.1 (PREDICTED: xanthoxin dehydrogenase [Cucumis melo] >TYJ99851.1 xanthoxin dehydrogenase [Cucumis melo var. makuwa])

HSP 1 Score: 502.3 bits (1292), Expect = 2.7e-138
Identity = 251/280 (89.64%), Postives = 267/280 (95.36%), Query Frame = 0

Query: 1   MATLQSSSLITQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDEPGYRLHENL 60
           MAT   SSL++QRLLGKVA VTGGASGIGE IVRLFHRHGAKVCFVDVQDE GYRL E+L
Sbjct: 1   MATPPISSLVSQRLLGKVAFVTGGASGIGEGIVRLFHRHGAKVCFVDVQDELGYRLLESL 60

Query: 61  GGDKDSNTYYSHCDVTVEEDIRRAVDLTVSKFGTLDIMVNNAGISGTPSSDIRNVDVSEF 120
           GGDKD N +YSHCDVTVE+D+RRAVDLTV++FGTLDIMVNNAGISGTPSSDIRNVD+SEF
Sbjct: 61  GGDKDPNIFYSHCDVTVEDDLRRAVDLTVTRFGTLDIMVNNAGISGTPSSDIRNVDISEF 120

Query: 121 ERVFDINVKAVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVLGLTR 180
           E+VFDINVKAVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAV+GLTR
Sbjct: 121 EKVFDINVKAVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVVGLTR 180

Query: 181 SVAAELGQHGIRVNCVSPYGVPTGLSVAHLPEEERTEDVFTDFREFAKKNANLQGVELEV 240
           SVAAELGQHGIRVNCVSPY VPT L+VAHLPE+ERTEDVFT FREFAKKNANLQGVEL V
Sbjct: 181 SVAAELGQHGIRVNCVSPYAVPTNLAVAHLPEDERTEDVFTGFREFAKKNANLQGVELTV 240

Query: 241 EDVANAVLFLASDEARYISGDNLLVDGGFTRVNHSLRVFK 281
           EDVANAVLFLAS++ARYISGDNLLVDGGFTRVNHSLRVF+
Sbjct: 241 EDVANAVLFLASEDARYISGDNLLVDGGFTRVNHSLRVFR 280

BLAST of Bhi12G000967 vs. NCBI nr
Match: XP_011655258.1 (xanthoxin dehydrogenase isoform X1 [Cucumis sativus] >KGN51108.1 hypothetical protein Csa_009294 [Cucumis sativus])

HSP 1 Score: 497.7 bits (1280), Expect = 6.7e-137
Identity = 249/280 (88.93%), Postives = 265/280 (94.64%), Query Frame = 0

Query: 1   MATLQSSSLITQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDEPGYRLHENL 60
           MAT   SSLI+QRLLGKVALVTGGASGIGE IVRLFHRHGAKV FVDVQDE GYRL E+L
Sbjct: 1   MATPPISSLISQRLLGKVALVTGGASGIGEGIVRLFHRHGAKVFFVDVQDELGYRLQESL 60

Query: 61  GGDKDSNTYYSHCDVTVEEDIRRAVDLTVSKFGTLDIMVNNAGISGTPSSDIRNVDVSEF 120
           GGDKDSN +YSHCDVTVE+D+RRAVDLTV+KFGTLDIMVNNAGISGTPSSDIRNVDVSEF
Sbjct: 61  GGDKDSNIFYSHCDVTVEDDVRRAVDLTVTKFGTLDIMVNNAGISGTPSSDIRNVDVSEF 120

Query: 121 ERVFDINVKAVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVLGLTR 180
           E+VFDINVK VFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHY+ SKHAV+GLTR
Sbjct: 121 EKVFDINVKGVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYISSKHAVVGLTR 180

Query: 181 SVAAELGQHGIRVNCVSPYGVPTGLSVAHLPEEERTEDVFTDFREFAKKNANLQGVELEV 240
           S+AAELGQHGIRVNCVSPY VPT L+VAHLPE+ERTED+FT FREFAKKNANLQGVEL V
Sbjct: 181 SIAAELGQHGIRVNCVSPYAVPTNLAVAHLPEDERTEDMFTGFREFAKKNANLQGVELTV 240

Query: 241 EDVANAVLFLASDEARYISGDNLLVDGGFTRVNHSLRVFK 281
           EDVANAVLFLAS++ARYISGDNLLVDGGFTRVNHS RVF+
Sbjct: 241 EDVANAVLFLASEDARYISGDNLLVDGGFTRVNHSFRVFR 280

BLAST of Bhi12G000967 vs. NCBI nr
Match: KAA0066045.1 (xanthoxin dehydrogenase [Cucumis melo var. makuwa])

HSP 1 Score: 491.9 bits (1265), Expect = 3.7e-135
Identity = 244/268 (91.04%), Postives = 258/268 (96.27%), Query Frame = 0

Query: 13  RLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDEPGYRLHENLGGDKDSNTYYSH 72
           RLLGKVA VTGGASGIGE IVRLFHRHGAKVCFVDVQDE GYRL E+LGGDKD N +YSH
Sbjct: 4   RLLGKVAFVTGGASGIGEGIVRLFHRHGAKVCFVDVQDELGYRLLESLGGDKDPNIFYSH 63

Query: 73  CDVTVEEDIRRAVDLTVSKFGTLDIMVNNAGISGTPSSDIRNVDVSEFERVFDINVKAVF 132
           CDVTVE+D+RRAVDLTV++FGTLDIMVNNAGISGTPSSDIRNVD+SEFE+VFDINVKAVF
Sbjct: 64  CDVTVEDDLRRAVDLTVTRFGTLDIMVNNAGISGTPSSDIRNVDISEFEKVFDINVKAVF 123

Query: 133 MGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVLGLTRSVAAELGQHGIR 192
           MGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAV+GLTRSVAAELGQHGIR
Sbjct: 124 MGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVVGLTRSVAAELGQHGIR 183

Query: 193 VNCVSPYGVPTGLSVAHLPEEERTEDVFTDFREFAKKNANLQGVELEVEDVANAVLFLAS 252
           VNCVSPY VPT L+VAHLPE+ERTEDVFT FREFAKKNANLQGVEL VEDVANAVLFLAS
Sbjct: 184 VNCVSPYAVPTNLAVAHLPEDERTEDVFTGFREFAKKNANLQGVELTVEDVANAVLFLAS 243

Query: 253 DEARYISGDNLLVDGGFTRVNHSLRVFK 281
           ++ARYISGDNLLVDGGFTRVNHSLRVF+
Sbjct: 244 EDARYISGDNLLVDGGFTRVNHSLRVFR 271

BLAST of Bhi12G000967 vs. NCBI nr
Match: XP_031742249.1 (xanthoxin dehydrogenase isoform X2 [Cucumis sativus])

HSP 1 Score: 486.9 bits (1252), Expect = 1.2e-133
Identity = 241/268 (89.93%), Postives = 256/268 (95.52%), Query Frame = 0

Query: 13  RLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDEPGYRLHENLGGDKDSNTYYSH 72
           RLLGKVALVTGGASGIGE IVRLFHRHGAKV FVDVQDE GYRL E+LGGDKDSN +YSH
Sbjct: 4   RLLGKVALVTGGASGIGEGIVRLFHRHGAKVFFVDVQDELGYRLQESLGGDKDSNIFYSH 63

Query: 73  CDVTVEEDIRRAVDLTVSKFGTLDIMVNNAGISGTPSSDIRNVDVSEFERVFDINVKAVF 132
           CDVTVE+D+RRAVDLTV+KFGTLDIMVNNAGISGTPSSDIRNVDVSEFE+VFDINVK VF
Sbjct: 64  CDVTVEDDVRRAVDLTVTKFGTLDIMVNNAGISGTPSSDIRNVDVSEFEKVFDINVKGVF 123

Query: 133 MGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVLGLTRSVAAELGQHGIR 192
           MGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHY+ SKHAV+GLTRS+AAELGQHGIR
Sbjct: 124 MGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYISSKHAVVGLTRSIAAELGQHGIR 183

Query: 193 VNCVSPYGVPTGLSVAHLPEEERTEDVFTDFREFAKKNANLQGVELEVEDVANAVLFLAS 252
           VNCVSPY VPT L+VAHLPE+ERTED+FT FREFAKKNANLQGVEL VEDVANAVLFLAS
Sbjct: 184 VNCVSPYAVPTNLAVAHLPEDERTEDMFTGFREFAKKNANLQGVELTVEDVANAVLFLAS 243

Query: 253 DEARYISGDNLLVDGGFTRVNHSLRVFK 281
           ++ARYISGDNLLVDGGFTRVNHS RVF+
Sbjct: 244 EDARYISGDNLLVDGGFTRVNHSFRVFR 271

BLAST of Bhi12G000967 vs. ExPASy TrEMBL
Match: A0A5D3BMA8 (Xanthoxin dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold446G00650 PE=4 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.3e-138
Identity = 251/280 (89.64%), Postives = 267/280 (95.36%), Query Frame = 0

Query: 1   MATLQSSSLITQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDEPGYRLHENL 60
           MAT   SSL++QRLLGKVA VTGGASGIGE IVRLFHRHGAKVCFVDVQDE GYRL E+L
Sbjct: 1   MATPPISSLVSQRLLGKVAFVTGGASGIGEGIVRLFHRHGAKVCFVDVQDELGYRLLESL 60

Query: 61  GGDKDSNTYYSHCDVTVEEDIRRAVDLTVSKFGTLDIMVNNAGISGTPSSDIRNVDVSEF 120
           GGDKD N +YSHCDVTVE+D+RRAVDLTV++FGTLDIMVNNAGISGTPSSDIRNVD+SEF
Sbjct: 61  GGDKDPNIFYSHCDVTVEDDLRRAVDLTVTRFGTLDIMVNNAGISGTPSSDIRNVDISEF 120

Query: 121 ERVFDINVKAVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVLGLTR 180
           E+VFDINVKAVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAV+GLTR
Sbjct: 121 EKVFDINVKAVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVVGLTR 180

Query: 181 SVAAELGQHGIRVNCVSPYGVPTGLSVAHLPEEERTEDVFTDFREFAKKNANLQGVELEV 240
           SVAAELGQHGIRVNCVSPY VPT L+VAHLPE+ERTEDVFT FREFAKKNANLQGVEL V
Sbjct: 181 SVAAELGQHGIRVNCVSPYAVPTNLAVAHLPEDERTEDVFTGFREFAKKNANLQGVELTV 240

Query: 241 EDVANAVLFLASDEARYISGDNLLVDGGFTRVNHSLRVFK 281
           EDVANAVLFLAS++ARYISGDNLLVDGGFTRVNHSLRVF+
Sbjct: 241 EDVANAVLFLASEDARYISGDNLLVDGGFTRVNHSLRVFR 280

BLAST of Bhi12G000967 vs. ExPASy TrEMBL
Match: A0A1S3CPE9 (xanthoxin dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103503321 PE=4 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.3e-138
Identity = 251/280 (89.64%), Postives = 267/280 (95.36%), Query Frame = 0

Query: 1   MATLQSSSLITQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDEPGYRLHENL 60
           MAT   SSL++QRLLGKVA VTGGASGIGE IVRLFHRHGAKVCFVDVQDE GYRL E+L
Sbjct: 1   MATPPISSLVSQRLLGKVAFVTGGASGIGEGIVRLFHRHGAKVCFVDVQDELGYRLLESL 60

Query: 61  GGDKDSNTYYSHCDVTVEEDIRRAVDLTVSKFGTLDIMVNNAGISGTPSSDIRNVDVSEF 120
           GGDKD N +YSHCDVTVE+D+RRAVDLTV++FGTLDIMVNNAGISGTPSSDIRNVD+SEF
Sbjct: 61  GGDKDPNIFYSHCDVTVEDDLRRAVDLTVTRFGTLDIMVNNAGISGTPSSDIRNVDISEF 120

Query: 121 ERVFDINVKAVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVLGLTR 180
           E+VFDINVKAVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAV+GLTR
Sbjct: 121 EKVFDINVKAVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVVGLTR 180

Query: 181 SVAAELGQHGIRVNCVSPYGVPTGLSVAHLPEEERTEDVFTDFREFAKKNANLQGVELEV 240
           SVAAELGQHGIRVNCVSPY VPT L+VAHLPE+ERTEDVFT FREFAKKNANLQGVEL V
Sbjct: 181 SVAAELGQHGIRVNCVSPYAVPTNLAVAHLPEDERTEDVFTGFREFAKKNANLQGVELTV 240

Query: 241 EDVANAVLFLASDEARYISGDNLLVDGGFTRVNHSLRVFK 281
           EDVANAVLFLAS++ARYISGDNLLVDGGFTRVNHSLRVF+
Sbjct: 241 EDVANAVLFLASEDARYISGDNLLVDGGFTRVNHSLRVFR 280

BLAST of Bhi12G000967 vs. ExPASy TrEMBL
Match: A0A0A0KNF1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G453160 PE=4 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 3.3e-137
Identity = 249/280 (88.93%), Postives = 265/280 (94.64%), Query Frame = 0

Query: 1   MATLQSSSLITQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDEPGYRLHENL 60
           MAT   SSLI+QRLLGKVALVTGGASGIGE IVRLFHRHGAKV FVDVQDE GYRL E+L
Sbjct: 1   MATPPISSLISQRLLGKVALVTGGASGIGEGIVRLFHRHGAKVFFVDVQDELGYRLQESL 60

Query: 61  GGDKDSNTYYSHCDVTVEEDIRRAVDLTVSKFGTLDIMVNNAGISGTPSSDIRNVDVSEF 120
           GGDKDSN +YSHCDVTVE+D+RRAVDLTV+KFGTLDIMVNNAGISGTPSSDIRNVDVSEF
Sbjct: 61  GGDKDSNIFYSHCDVTVEDDVRRAVDLTVTKFGTLDIMVNNAGISGTPSSDIRNVDVSEF 120

Query: 121 ERVFDINVKAVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVLGLTR 180
           E+VFDINVK VFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHY+ SKHAV+GLTR
Sbjct: 121 EKVFDINVKGVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYISSKHAVVGLTR 180

Query: 181 SVAAELGQHGIRVNCVSPYGVPTGLSVAHLPEEERTEDVFTDFREFAKKNANLQGVELEV 240
           S+AAELGQHGIRVNCVSPY VPT L+VAHLPE+ERTED+FT FREFAKKNANLQGVEL V
Sbjct: 181 SIAAELGQHGIRVNCVSPYAVPTNLAVAHLPEDERTEDMFTGFREFAKKNANLQGVELTV 240

Query: 241 EDVANAVLFLASDEARYISGDNLLVDGGFTRVNHSLRVFK 281
           EDVANAVLFLAS++ARYISGDNLLVDGGFTRVNHS RVF+
Sbjct: 241 EDVANAVLFLASEDARYISGDNLLVDGGFTRVNHSFRVFR 280

BLAST of Bhi12G000967 vs. ExPASy TrEMBL
Match: A0A5A7VJY7 (Xanthoxin dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold21G00190 PE=4 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 1.8e-135
Identity = 244/268 (91.04%), Postives = 258/268 (96.27%), Query Frame = 0

Query: 13  RLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDEPGYRLHENLGGDKDSNTYYSH 72
           RLLGKVA VTGGASGIGE IVRLFHRHGAKVCFVDVQDE GYRL E+LGGDKD N +YSH
Sbjct: 4   RLLGKVAFVTGGASGIGEGIVRLFHRHGAKVCFVDVQDELGYRLLESLGGDKDPNIFYSH 63

Query: 73  CDVTVEEDIRRAVDLTVSKFGTLDIMVNNAGISGTPSSDIRNVDVSEFERVFDINVKAVF 132
           CDVTVE+D+RRAVDLTV++FGTLDIMVNNAGISGTPSSDIRNVD+SEFE+VFDINVKAVF
Sbjct: 64  CDVTVEDDLRRAVDLTVTRFGTLDIMVNNAGISGTPSSDIRNVDISEFEKVFDINVKAVF 123

Query: 133 MGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVLGLTRSVAAELGQHGIR 192
           MGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAV+GLTRSVAAELGQHGIR
Sbjct: 124 MGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVVGLTRSVAAELGQHGIR 183

Query: 193 VNCVSPYGVPTGLSVAHLPEEERTEDVFTDFREFAKKNANLQGVELEVEDVANAVLFLAS 252
           VNCVSPY VPT L+VAHLPE+ERTEDVFT FREFAKKNANLQGVEL VEDVANAVLFLAS
Sbjct: 184 VNCVSPYAVPTNLAVAHLPEDERTEDVFTGFREFAKKNANLQGVELTVEDVANAVLFLAS 243

Query: 253 DEARYISGDNLLVDGGFTRVNHSLRVFK 281
           ++ARYISGDNLLVDGGFTRVNHSLRVF+
Sbjct: 244 EDARYISGDNLLVDGGFTRVNHSLRVFR 271

BLAST of Bhi12G000967 vs. ExPASy TrEMBL
Match: A0A6J1JZF0 (xanthoxin dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111489670 PE=4 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 5.9e-131
Identity = 243/282 (86.17%), Postives = 262/282 (92.91%), Query Frame = 0

Query: 1   MATLQ--SSSLITQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDEPGYRLHE 60
           MAT +  SSSL+ QRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDE G  LHE
Sbjct: 1   MATAESSSSSLVPQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELGRCLHE 60

Query: 61  NLGGDKDSNTYYSHCDVTVEEDIRRAVDLTVSKFGTLDIMVNNAGISGTPSSDIRNVDVS 120
           +LGG+ DSNT+YSHCDVTVE+D+RRAVD+TVSKFGTLDIMVNNAGISGTPSSDIR+VDVS
Sbjct: 61  DLGGNDDSNTFYSHCDVTVEDDVRRAVDVTVSKFGTLDIMVNNAGISGTPSSDIRSVDVS 120

Query: 121 EFERVFDINVKAVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVLGL 180
           EFE+VF+INVKAVFMGMKYAASVMIP KQGSIISL SVGSVIGGIGPHHY+ SKHAVLGL
Sbjct: 121 EFEKVFNINVKAVFMGMKYAASVMIPNKQGSIISLASVGSVIGGIGPHHYISSKHAVLGL 180

Query: 181 TRSVAAELGQHGIRVNCVSPYGVPTGLSVAHLPEEERTEDVFTDFREFAKKNANLQGVEL 240
           TRSVAAELGQ+GIRVNCVSPY VPT L+ AHL EEERTEDV T FR+F  KNANLQGV+L
Sbjct: 181 TRSVAAELGQYGIRVNCVSPYAVPTSLAFAHLHEEERTEDVITGFRQFTGKNANLQGVDL 240

Query: 241 EVEDVANAVLFLASDEARYISGDNLLVDGGFTRVNHSLRVFK 281
           EVEDVANAVLFLAS+EARYISGDNLLVDGGF+ VNHSLRVF+
Sbjct: 241 EVEDVANAVLFLASNEARYISGDNLLVDGGFSCVNHSLRVFR 282

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G52340.11.0e-10668.73NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G51680.13.8e-6145.96NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G26770.13.3e-5745.83NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G29250.22.1e-5646.74NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G29250.12.8e-5645.72NAD(P)-binding Rossmann-fold superfamily protein [more]
Match NameE-valueIdentityDescription
Q9C8261.4e-10568.73Xanthoxin dehydrogenase OS=Arabidopsis thaliana OX=3702 GN=ABA2 PE=1 SV=1[more]
F1SWA02.3e-9563.81Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1[more]
Q94KL79.3e-7353.74Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P... [more]
Q7FAE18.7e-6348.71Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ... [more]
P501601.6e-6147.16Sex determination protein tasselseed-2 OS=Zea mays OX=4577 GN=TS2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038906598.11.3e-153100.00xanthoxin dehydrogenase [Benincasa hispida][more]
XP_008465699.12.7e-13889.64PREDICTED: xanthoxin dehydrogenase [Cucumis melo] >TYJ99851.1 xanthoxin dehydrog... [more]
XP_011655258.16.7e-13788.93xanthoxin dehydrogenase isoform X1 [Cucumis sativus] >KGN51108.1 hypothetical pr... [more]
KAA0066045.13.7e-13591.04xanthoxin dehydrogenase [Cucumis melo var. makuwa][more]
XP_031742249.11.2e-13389.93xanthoxin dehydrogenase isoform X2 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A5D3BMA81.3e-13889.64Xanthoxin dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3CPE91.3e-13889.64xanthoxin dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103503321 PE=4 SV=1[more]
A0A0A0KNF13.3e-13788.93Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G453160 PE=4 SV=1[more]
A0A5A7VJY71.8e-13591.04Xanthoxin dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A6J1JZF05.9e-13186.17xanthoxin dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111489670 PE=4 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 168..187
score: 39.43
coord: 148..156
score: 44.81
coord: 93..104
score: 57.32
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 93..104
score: 61.97
coord: 189..206
score: 40.75
coord: 168..187
score: 41.8
coord: 233..253
score: 35.68
coord: 18..35
score: 55.84
coord: 142..158
score: 30.01
NoneNo IPR availableGENE3D3.40.50.720coord: 4..279
e-value: 2.7E-78
score: 264.8
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 23..270
e-value: 2.6E-64
score: 217.0
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 20..247
e-value: 2.0E-17
score: 61.2
NoneNo IPR availablePIRSRPIRSR629511-2PIRSR629511-2coord: 12..269
e-value: 3.4E-32
score: 109.4
NoneNo IPR availablePANTHERPTHR42820SHORT-CHAIN DEHYDROGENASE REDUCTASEcoord: 7..280
NoneNo IPR availablePANTHERPTHR42820:SF1XANTHOXIN DEHYDROGENASEcoord: 7..280
NoneNo IPR availableCDDcd05326secoisolariciresinol-DH_like_SDR_ccoord: 13..270
e-value: 1.35102E-122
score: 348.291
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 16..271

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi12M000967Bhi12M000967mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009688 abscisic acid biosynthetic process
cellular_component GO:0005829 cytosol
molecular_function GO:0010301 xanthoxin dehydrogenase activity
molecular_function GO:0016491 oxidoreductase activity