Bhi12G000923 (gene) Wax gourd (B227) v1

BLAST of Bhi12G000923 vs. TAIR 10
Match: AT1G68910.1 (WPP domain-interacting protein 2 )

HSP 1 Score: 461.5 bits (1186), Expect = 1.0e-129
Identity = 283/538 (52.60%), Postives = 382/538 (71.00%), Query Frame = 0

Query: 19  QEMENTMKTLTKLELDLAYSSEKLMNLHVLLMCLLTQENDFEAMDLENEYIPDDSFGKVL 78
           +E +  ++ LTKLE+D AY+SEKLMNLHVLLM LL  +ND E M   +      SF K L
Sbjct: 18  EEEQEMLRMLTKLEIDSAYTSEKLMNLHVLLMHLLAWDNDLEGMGTLDS--SPASFEKAL 77

Query: 79  AFDFLSGFLDSEVRELDSFMDKLEAEIVDARGMVSSCRQSSEVFSVVEEKLLDSEKSLVQ 138
            FD L G L+SEV+E+D  +D LEA+IVD    +SSC+  +  + V+E KL +S +SL Q
Sbjct: 78  TFDLLCGILESEVKEVDEVLDVLEAQIVDTSYKISSCKHGN--YIVIEGKLGESAESLKQ 137

Query: 139 SQQQILEVKMQSAKLQRIVLSSGNWRLEDPVMSSQNDQVLLNINGKSNTMTEQQRHILRM 198
           S+ Q+ E+ +Q A+L+R +    N        S   + V L           + ++ LRM
Sbjct: 138 SRGQVSEITLQLAQLRRTLHYIRNG------TSENEESVELRQKYALKPSDLRHKNALRM 197

Query: 199 LEKSLARELDLEKKLSESKQREEELRMKLHYTEQVALRMEEAAEVVWGRFLEADNSVEIL 258
           LEKSL+REL+LEKKL E +Q EE+L++KLHYTE+V+ RMEEA+E +WGRFLEADNS E+L
Sbjct: 198 LEKSLSRELELEKKLMEFQQNEEQLKLKLHYTEEVSSRMEEASEFIWGRFLEADNSSEVL 257

Query: 259 MGISKELVGRLQLVQFNLHGSFQRENEVKTKCQDWTEQLKAKEVAIQKLE---KRNAELL 318
            GISKELVGRLQ++QF+L+GS QRE+E+K+K +D T QL+AK++ +QKLE     N+E++
Sbjct: 258 TGISKELVGRLQILQFSLNGSAQRESELKSKLEDCTVQLEAKDLLVQKLEGTISENSEIV 317

Query: 319 IAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEMENLVESLKESICISE 378
               +E+  LRE VKS E++LK + L+LKS   S +     L EMEN  ES+KE++  +E
Sbjct: 318 ----SEVLTLREYVKSAEQKLKNTDLELKSVNASKQEILVHLAEMENANESVKENLFEAE 377

Query: 379 NRAESVEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQLRQLEIQLQHAKSSSE 438
           +RAES EAK+ +L   NLELTEE++FLKD+   K KKV SLEKQ+R+LE+Q+Q++K SSE
Sbjct: 378 SRAESGEAKIKELDAANLELTEELNFLKDADDKKTKKVNSLEKQVRELEVQVQNSKVSSE 437

Query: 439 ASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIILSETNFELNKELTSLKA 498
           A+QEQQNMLYSAIWDMETLIEDLKSK SKAES+T++ EE CI+LS TN ELNK+++ L+ 
Sbjct: 438 ANQEQQNMLYSAIWDMETLIEDLKSKASKAESRTETVEEQCIVLSTTNSELNKDVSFLRQ 497

Query: 499 RVEFLEKALDQANGEKYTNANKVNLSSKFIMDMVLQLAVERDRIQSQLSILTNDNKAL 554
           + + LE  LD AN EK   A ++   +K +MDM+LQL+ ER+RIQ QL  L  +NK L
Sbjct: 498 KAKSLEAMLDLANNEKERYAQEITTRNKVLMDMMLQLSSERERIQEQLYSLAKENKIL 541

BLAST of Bhi12G000923 vs. TAIR 10
Match: AT1G68910.2 (WPP domain-interacting protein 2 )

HSP 1 Score: 461.5 bits (1186), Expect = 1.0e-129
Identity = 283/538 (52.60%), Postives = 382/538 (71.00%), Query Frame = 0

Query: 19  QEMENTMKTLTKLELDLAYSSEKLMNLHVLLMCLLTQENDFEAMDLENEYIPDDSFGKVL 78
           +E +  ++ LTKLE+D AY+SEKLMNLHVLLM LL  +ND E M   +      SF K L
Sbjct: 18  EEEQEMLRMLTKLEIDSAYTSEKLMNLHVLLMHLLAWDNDLEGMGTLDS--SPASFEKAL 77

Query: 79  AFDFLSGFLDSEVRELDSFMDKLEAEIVDARGMVSSCRQSSEVFSVVEEKLLDSEKSLVQ 138
            FD L G L+SEV+E+D  +D LEA+IVD    +SSC+  +  + V+E KL +S +SL Q
Sbjct: 78  TFDLLCGILESEVKEVDEVLDVLEAQIVDTSYKISSCKHGN--YIVIEGKLGESAESLKQ 137

Query: 139 SQQQILEVKMQSAKLQRIVLSSGNWRLEDPVMSSQNDQVLLNINGKSNTMTEQQRHILRM 198
           S+ Q+ E+ +Q A+L+R +    N        S   + V L           + ++ LRM
Sbjct: 138 SRGQVSEITLQLAQLRRTLHYIRNG------TSENEESVELRQKYALKPSDLRHKNALRM 197

Query: 199 LEKSLARELDLEKKLSESKQREEELRMKLHYTEQVALRMEEAAEVVWGRFLEADNSVEIL 258
           LEKSL+REL+LEKKL E +Q EE+L++KLHYTE+V+ RMEEA+E +WGRFLEADNS E+L
Sbjct: 198 LEKSLSRELELEKKLMEFQQNEEQLKLKLHYTEEVSSRMEEASEFIWGRFLEADNSSEVL 257

Query: 259 MGISKELVGRLQLVQFNLHGSFQRENEVKTKCQDWTEQLKAKEVAIQKLE---KRNAELL 318
            GISKELVGRLQ++QF+L+GS QRE+E+K+K +D T QL+AK++ +QKLE     N+E++
Sbjct: 258 TGISKELVGRLQILQFSLNGSAQRESELKSKLEDCTVQLEAKDLLVQKLEGTISENSEIV 317

Query: 319 IAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEMENLVESLKESICISE 378
               +E+  LRE VKS E++LK + L+LKS   S +     L EMEN  ES+KE++  +E
Sbjct: 318 ----SEVLTLREYVKSAEQKLKNTDLELKSVNASKQEILVHLAEMENANESVKENLFEAE 377

Query: 379 NRAESVEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQLRQLEIQLQHAKSSSE 438
           +RAES EAK+ +L   NLELTEE++FLKD+   K KKV SLEKQ+R+LE+Q+Q++K SSE
Sbjct: 378 SRAESGEAKIKELDAANLELTEELNFLKDADDKKTKKVNSLEKQVRELEVQVQNSKVSSE 437

Query: 439 ASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIILSETNFELNKELTSLKA 498
           A+QEQQNMLYSAIWDMETLIEDLKSK SKAES+T++ EE CI+LS TN ELNK+++ L+ 
Sbjct: 438 ANQEQQNMLYSAIWDMETLIEDLKSKASKAESRTETVEEQCIVLSTTNSELNKDVSFLRQ 497

Query: 499 RVEFLEKALDQANGEKYTNANKVNLSSKFIMDMVLQLAVERDRIQSQLSILTNDNKAL 554
           + + LE  LD AN EK   A ++   +K +MDM+LQL+ ER+RIQ QL  L  +NK L
Sbjct: 498 KAKSLEAMLDLANNEKERYAQEITTRNKVLMDMMLQLSSERERIQEQLYSLAKENKIL 541

BLAST of Bhi12G000923 vs. TAIR 10
Match: AT1G68910.3 (WPP domain-interacting protein 2 )

HSP 1 Score: 461.5 bits (1186), Expect = 1.0e-129
Identity = 283/538 (52.60%), Postives = 382/538 (71.00%), Query Frame = 0

Query: 19  QEMENTMKTLTKLELDLAYSSEKLMNLHVLLMCLLTQENDFEAMDLENEYIPDDSFGKVL 78
           +E +  ++ LTKLE+D AY+SEKLMNLHVLLM LL  +ND E M   +      SF K L
Sbjct: 18  EEEQEMLRMLTKLEIDSAYTSEKLMNLHVLLMHLLAWDNDLEGMGTLDS--SPASFEKAL 77

Query: 79  AFDFLSGFLDSEVRELDSFMDKLEAEIVDARGMVSSCRQSSEVFSVVEEKLLDSEKSLVQ 138
            FD L G L+SEV+E+D  +D LEA+IVD    +SSC+  +  + V+E KL +S +SL Q
Sbjct: 78  TFDLLCGILESEVKEVDEVLDVLEAQIVDTSYKISSCKHGN--YIVIEGKLGESAESLKQ 137

Query: 139 SQQQILEVKMQSAKLQRIVLSSGNWRLEDPVMSSQNDQVLLNINGKSNTMTEQQRHILRM 198
           S+ Q+ E+ +Q A+L+R +    N        S   + V L           + ++ LRM
Sbjct: 138 SRGQVSEITLQLAQLRRTLHYIRNG------TSENEESVELRQKYALKPSDLRHKNALRM 197

Query: 199 LEKSLARELDLEKKLSESKQREEELRMKLHYTEQVALRMEEAAEVVWGRFLEADNSVEIL 258
           LEKSL+REL+LEKKL E +Q EE+L++KLHYTE+V+ RMEEA+E +WGRFLEADNS E+L
Sbjct: 198 LEKSLSRELELEKKLMEFQQNEEQLKLKLHYTEEVSSRMEEASEFIWGRFLEADNSSEVL 257

Query: 259 MGISKELVGRLQLVQFNLHGSFQRENEVKTKCQDWTEQLKAKEVAIQKLE---KRNAELL 318
            GISKELVGRLQ++QF+L+GS QRE+E+K+K +D T QL+AK++ +QKLE     N+E++
Sbjct: 258 TGISKELVGRLQILQFSLNGSAQRESELKSKLEDCTVQLEAKDLLVQKLEGTISENSEIV 317

Query: 319 IAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEMENLVESLKESICISE 378
               +E+  LRE VKS E++LK + L+LKS   S +     L EMEN  ES+KE++  +E
Sbjct: 318 ----SEVLTLREYVKSAEQKLKNTDLELKSVNASKQEILVHLAEMENANESVKENLFEAE 377

Query: 379 NRAESVEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQLRQLEIQLQHAKSSSE 438
           +RAES EAK+ +L   NLELTEE++FLKD+   K KKV SLEKQ+R+LE+Q+Q++K SSE
Sbjct: 378 SRAESGEAKIKELDAANLELTEELNFLKDADDKKTKKVNSLEKQVRELEVQVQNSKVSSE 437

Query: 439 ASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIILSETNFELNKELTSLKA 498
           A+QEQQNMLYSAIWDMETLIEDLKSK SKAES+T++ EE CI+LS TN ELNK+++ L+ 
Sbjct: 438 ANQEQQNMLYSAIWDMETLIEDLKSKASKAESRTETVEEQCIVLSTTNSELNKDVSFLRQ 497

Query: 499 RVEFLEKALDQANGEKYTNANKVNLSSKFIMDMVLQLAVERDRIQSQLSILTNDNKAL 554
           + + LE  LD AN EK   A ++   +K +MDM+LQL+ ER+RIQ QL  L  +NK L
Sbjct: 498 KAKSLEAMLDLANNEKERYAQEITTRNKVLMDMMLQLSSERERIQEQLYSLAKENKIL 541

BLAST of Bhi12G000923 vs. TAIR 10
Match: AT5G11390.1 (WPP domain-interacting protein 1 )

HSP 1 Score: 357.5 bits (916), Expect = 2.1e-98
Identity = 245/576 (42.53%), Postives = 355/576 (61.63%), Query Frame = 0

Query: 20  EMENTMKTLTKLELDLAYSSEKLMNLHVLLMCLLTQENDFEAM---DLENEYIP------ 79
           ++ +  + LTK+ELD A+ SEKL+NL +L M L T+ENDFE+      E+E  P      
Sbjct: 44  QLSSAGEVLTKVELDFAFVSEKLVNLSLLTMQLGTRENDFESFVSKKEEDEEEPSSNVDD 103

Query: 80  -DDSFGKVLAFDFLSGFLDSEVRELDSFMDKLEAEIVDARGMVSSCRQSSEVFSVVEEKL 139
            DDS  K L FD LS  L+SEV+EL+S +  L+ EI  AR M+S  +   E F  +E KL
Sbjct: 104 DDDSAEKALEFDLLSSILNSEVKELESLLGFLQNEIQSARVMISPFQHDGEAFLDLEGKL 163

Query: 140 LDSEKSLVQSQQQILEVKMQSAKLQRI---VLSSGNWRLEDPVMSSQNDQVLLNINGKSN 199
            D+E+SL Q  +Q++E+K QS+  QR+   +   G+W        SQND    +++ K N
Sbjct: 164 NDTEQSLGQLMEQVVEMKKQSSNFQRLSSGLDEQGSWS-GGQTSVSQNDGEFGDLSAKIN 223

Query: 200 TMT-EQQRHILRMLEKSLARELDLEKKLSESKQREEELRMKLHYTEQVALRMEEAAEVVW 259
             T +QQR++LRMLEKSLA+E++LEKKLSES+  E EL MKL+ +EQ  + MEE  E  +
Sbjct: 224 MQTADQQRNVLRMLEKSLAKEMELEKKLSESRNTERELEMKLYSSEQDVVYMEEVTEDAF 283

Query: 260 GRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENEVKTKCQDWTEQLKAKEVAIQ 319
            R+LEADN+ E+  G SKE+ G+LQ++QFNL GSF+RE+ +K+K  D  E+L+AKE A+ 
Sbjct: 284 SRWLEADNAAEVFKGTSKEMSGKLQILQFNLSGSFKREDNLKSKLVDSKERLEAKECALH 343

Query: 320 KLEKRNAEL---------------------LIAKNAELDKLREEVKSLEEQLKESRLDLK 379
           KL+  NA L                     LI  N E   L E+V SLEEQL E  +  +
Sbjct: 344 KLDSSNARLADFLVAQTEGLKESLQEAEEKLILLNTENSTLSEKVSSLEEQLNEYGIQTE 403

Query: 380 SAYDSNEASQDQLIEMENLVESLKESICISENRAESVEAKLTQLQETNLELTEEVSFLKD 439
              D++  S   + ++E + E LK+ +  +E RAE  E+K   L+E+  EL +E+   +D
Sbjct: 404 ---DADATSGALITDLERINEELKDKLAKTEARAEETESKCKILEESKKELQDELGNFRD 463

Query: 440 SASNKEKKVGSLEKQLRQLEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSK 499
                  K+ SLEK LR  ++QL+HA ++ EAS+E+QN+LYS + DME +IEDLKSKV K
Sbjct: 464 KGFTIH-KLASLEKHLRDSDLQLEHAVAAVEASKEKQNLLYSTVSDMEDVIEDLKSKVLK 523

Query: 500 AESKTDSAEEHCIILSETNFELNKELTSLKARVEFLEKALDQANGEKYTNANKVNLSSKF 559
           AE++ D  EE  I++SE+N E+N+EL   K R++  EK L QA   K   A  + + +K 
Sbjct: 524 AENRADITEEKLIMVSESNAEVNEELKFFKGRLKEGEKYLQQAEERKLRTAKDIGVHNKI 583

Query: 560 IMDMVLQLAVERDRIQSQLSILTNDNKALIEKLRTV 561
           +  +V+QLA ER+R+  Q++ L+ +N  L+ KL+ V
Sbjct: 584 MKKLVMQLAAERERLHKQITNLSRENCVLMVKLKKV 614

BLAST of Bhi12G000923 vs. TAIR 10
Match: AT2G32240.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: response to cadmium ion; LOCATED IN: plasma membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Prefoldin (InterPro:IPR009053); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G05320.3); Has 470429 Blast hits to 168274 proteins in 4282 species: Archae - 6896; Bacteria - 131956; Metazoa - 175525; Fungi - 33166; Plants - 25441; Viruses - 2243; Other Eukaryotes - 95202 (source: NCBI BLink). )

HSP 1 Score: 55.1 bits (131), Expect = 2.3e-07
Identity = 109/497 (21.93%), Postives = 205/497 (41.25%), Query Frame = 0

Query: 115  CRQSSEVFSVVEEKLLDSEKSLVQSQQQILEVKMQSAKLQRIVLSSGNWRLEDPVMSSQN 174
            C+ S       E +L D E  L   + +I E++ Q + L++                 ++
Sbjct: 615  CQSSQSKHEDAEGRLKDLELLLQTEKYRIQELEEQVSSLEK-----------------KH 674

Query: 175  DQVLLNINGKSNTMTEQQRHILRMLEKSLARELDLEKKLSESKQREEELRMKLHYTEQVA 234
             +   +  G    + E Q      LE    +   LE  L+ + + E+EL   L+      
Sbjct: 675  GETEADSKGYLGQVAELQ----STLEAFQVKSSSLEAALNIATENEKELTENLNAVTSEK 734

Query: 235  LRMEEAAEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENEVKTKCQDWT 294
             ++E   +    +  E++N +E +        G+L+ ++ +L  +  +E+EV  K +   
Sbjct: 735  KKLEATVDEYSVKISESENLLESIRNELNVTQGKLESIENDLKAAGLQESEVMEKLKSAE 794

Query: 295  EQLKAKEVAI-------QKLEKRNAELLIAKNAELDKLREEVKSLEEQLKESRLDLKSAY 354
            E L+ K   I        +LE  +  L I     L K  EE  S + +       L+   
Sbjct: 795  ESLEQKGREIDEATTKRMELEALHQSLSIDSEHRLQKAMEEFTSRDSEASSLTEKLRDLE 854

Query: 355  DSNEASQDQLIEMENLVESLKESICISENR---AESVEAKL----TQLQETNLELTEEVS 414
               ++ ++QL E      SLKE +  +  R   AESV  KL     Q QE +L+ + E  
Sbjct: 855  GKIKSYEEQLAEASGKSSSLKEKLEQTLGRLAAAESVNEKLKQEFDQAQEKSLQSSSESE 914

Query: 415  FLKDSASNKEKKV---------GSLEKQ--LRQLEIQLQH--------------AKSSSE 474
             L ++ +  + K+         GS+EK+  L++LE  ++                K+   
Sbjct: 915  LLAETNNQLKIKIQELEGLIGSGSVEKETALKRLEEAIERFNQKETESSDLVEKLKTHEN 974

Query: 475  ASQEQQNMLYSA----------IWDMETLIEDLKSKVSKAESKTDSAEEHCIILSETNFE 534
              +E + + + A          + D  + +++L+S + +  +K    E+    L+E N +
Sbjct: 975  QIEEYKKLAHEASGVADTRKVELEDALSKLKNLESTIEELGAKCQGLEKESGDLAEVNLK 1034

Query: 535  LNKELTSLKARVEFLEKALDQANGEKYTNANKVNLSSKFIMDMVLQLAVERDRIQSQLSI 563
            LN EL +  +    L+  L     EK   AN++  S   I D+  QL  E +++QSQ+S 
Sbjct: 1035 LNLELANHGSEANELQTKLSALEAEKEQTANELEASKTTIEDLTKQLTSEGEKLQSQISS 1090

BLAST of Bhi12G000923 vs. ExPASy Swiss-Prot
Match: A8MQR0 (WPP domain-interacting tail-anchored protein 2 OS=Arabidopsis thaliana OX=3702 GN=WIT2 PE=1 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 1.5e-128
Identity = 283/538 (52.60%), Postives = 382/538 (71.00%), Query Frame = 0

Query: 19  QEMENTMKTLTKLELDLAYSSEKLMNLHVLLMCLLTQENDFEAMDLENEYIPDDSFGKVL 78
           +E +  ++ LTKLE+D AY+SEKLMNLHVLLM LL  +ND E M   +      SF K L
Sbjct: 18  EEEQEMLRMLTKLEIDSAYTSEKLMNLHVLLMHLLAWDNDLEGMGTLDS--SPASFEKAL 77

Query: 79  AFDFLSGFLDSEVRELDSFMDKLEAEIVDARGMVSSCRQSSEVFSVVEEKLLDSEKSLVQ 138
            FD L G L+SEV+E+D  +D LEA+IVD    +SSC+  +  + V+E KL +S +SL Q
Sbjct: 78  TFDLLCGILESEVKEVDEVLDVLEAQIVDTSYKISSCKHGN--YIVIEGKLGESAESLKQ 137

Query: 139 SQQQILEVKMQSAKLQRIVLSSGNWRLEDPVMSSQNDQVLLNINGKSNTMTEQQRHILRM 198
           S+ Q+ E+ +Q A+L+R +    N        S   + V L           + ++ LRM
Sbjct: 138 SRGQVSEITLQLAQLRRTLHYIRNG------TSENEESVELRQKYALKPSDLRHKNALRM 197

Query: 199 LEKSLARELDLEKKLSESKQREEELRMKLHYTEQVALRMEEAAEVVWGRFLEADNSVEIL 258
           LEKSL+REL+LEKKL E +Q EE+L++KLHYTE+V+ RMEEA+E +WGRFLEADNS E+L
Sbjct: 198 LEKSLSRELELEKKLMEFQQNEEQLKLKLHYTEEVSSRMEEASEFIWGRFLEADNSSEVL 257

Query: 259 MGISKELVGRLQLVQFNLHGSFQRENEVKTKCQDWTEQLKAKEVAIQKLE---KRNAELL 318
            GISKELVGRLQ++QF+L+GS QRE+E+K+K +D T QL+AK++ +QKLE     N+E++
Sbjct: 258 TGISKELVGRLQILQFSLNGSAQRESELKSKLEDCTVQLEAKDLLVQKLEGTISENSEIV 317

Query: 319 IAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEMENLVESLKESICISE 378
               +E+  LRE VKS E++LK + L+LKS   S +     L EMEN  ES+KE++  +E
Sbjct: 318 ----SEVLTLREYVKSAEQKLKNTDLELKSVNASKQEILVHLAEMENANESVKENLFEAE 377

Query: 379 NRAESVEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQLRQLEIQLQHAKSSSE 438
           +RAES EAK+ +L   NLELTEE++FLKD+   K KKV SLEKQ+R+LE+Q+Q++K SSE
Sbjct: 378 SRAESGEAKIKELDAANLELTEELNFLKDADDKKTKKVNSLEKQVRELEVQVQNSKVSSE 437

Query: 439 ASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIILSETNFELNKELTSLKA 498
           A+QEQQNMLYSAIWDMETLIEDLKSK SKAES+T++ EE CI+LS TN ELNK+++ L+ 
Sbjct: 438 ANQEQQNMLYSAIWDMETLIEDLKSKASKAESRTETVEEQCIVLSTTNSELNKDVSFLRQ 497

Query: 499 RVEFLEKALDQANGEKYTNANKVNLSSKFIMDMVLQLAVERDRIQSQLSILTNDNKAL 554
           + + LE  LD AN EK   A ++   +K +MDM+LQL+ ER+RIQ QL  L  +NK L
Sbjct: 498 KAKSLEAMLDLANNEKERYAQEITTRNKVLMDMMLQLSSERERIQEQLYSLAKENKIL 541

BLAST of Bhi12G000923 vs. ExPASy Swiss-Prot
Match: Q8L7E5 (WPP domain-interacting tail-anchored protein 1 OS=Arabidopsis thaliana OX=3702 GN=WIT1 PE=1 SV=2)

HSP 1 Score: 357.5 bits (916), Expect = 3.0e-97
Identity = 245/576 (42.53%), Postives = 355/576 (61.63%), Query Frame = 0

Query: 20  EMENTMKTLTKLELDLAYSSEKLMNLHVLLMCLLTQENDFEAM---DLENEYIP------ 79
           ++ +  + LTK+ELD A+ SEKL+NL +L M L T+ENDFE+      E+E  P      
Sbjct: 44  QLSSAGEVLTKVELDFAFVSEKLVNLSLLTMQLGTRENDFESFVSKKEEDEEEPSSNVDD 103

Query: 80  -DDSFGKVLAFDFLSGFLDSEVRELDSFMDKLEAEIVDARGMVSSCRQSSEVFSVVEEKL 139
            DDS  K L FD LS  L+SEV+EL+S +  L+ EI  AR M+S  +   E F  +E KL
Sbjct: 104 DDDSAEKALEFDLLSSILNSEVKELESLLGFLQNEIQSARVMISPFQHDGEAFLDLEGKL 163

Query: 140 LDSEKSLVQSQQQILEVKMQSAKLQRI---VLSSGNWRLEDPVMSSQNDQVLLNINGKSN 199
            D+E+SL Q  +Q++E+K QS+  QR+   +   G+W        SQND    +++ K N
Sbjct: 164 NDTEQSLGQLMEQVVEMKKQSSNFQRLSSGLDEQGSWS-GGQTSVSQNDGEFGDLSAKIN 223

Query: 200 TMT-EQQRHILRMLEKSLARELDLEKKLSESKQREEELRMKLHYTEQVALRMEEAAEVVW 259
             T +QQR++LRMLEKSLA+E++LEKKLSES+  E EL MKL+ +EQ  + MEE  E  +
Sbjct: 224 MQTADQQRNVLRMLEKSLAKEMELEKKLSESRNTERELEMKLYSSEQDVVYMEEVTEDAF 283

Query: 260 GRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENEVKTKCQDWTEQLKAKEVAIQ 319
            R+LEADN+ E+  G SKE+ G+LQ++QFNL GSF+RE+ +K+K  D  E+L+AKE A+ 
Sbjct: 284 SRWLEADNAAEVFKGTSKEMSGKLQILQFNLSGSFKREDNLKSKLVDSKERLEAKECALH 343

Query: 320 KLEKRNAEL---------------------LIAKNAELDKLREEVKSLEEQLKESRLDLK 379
           KL+  NA L                     LI  N E   L E+V SLEEQL E  +  +
Sbjct: 344 KLDSSNARLADFLVAQTEGLKESLQEAEEKLILLNTENSTLSEKVSSLEEQLNEYGIQTE 403

Query: 380 SAYDSNEASQDQLIEMENLVESLKESICISENRAESVEAKLTQLQETNLELTEEVSFLKD 439
              D++  S   + ++E + E LK+ +  +E RAE  E+K   L+E+  EL +E+   +D
Sbjct: 404 ---DADATSGALITDLERINEELKDKLAKTEARAEETESKCKILEESKKELQDELGNFRD 463

Query: 440 SASNKEKKVGSLEKQLRQLEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSK 499
                  K+ SLEK LR  ++QL+HA ++ EAS+E+QN+LYS + DME +IEDLKSKV K
Sbjct: 464 KGFTIH-KLASLEKHLRDSDLQLEHAVAAVEASKEKQNLLYSTVSDMEDVIEDLKSKVLK 523

Query: 500 AESKTDSAEEHCIILSETNFELNKELTSLKARVEFLEKALDQANGEKYTNANKVNLSSKF 559
           AE++ D  EE  I++SE+N E+N+EL   K R++  EK L QA   K   A  + + +K 
Sbjct: 524 AENRADITEEKLIMVSESNAEVNEELKFFKGRLKEGEKYLQQAEERKLRTAKDIGVHNKI 583

Query: 560 IMDMVLQLAVERDRIQSQLSILTNDNKALIEKLRTV 561
           +  +V+QLA ER+R+  Q++ L+ +N  L+ KL+ V
Sbjct: 584 MKKLVMQLAAERERLHKQITNLSRENCVLMVKLKKV 614

BLAST of Bhi12G000923 vs. ExPASy Swiss-Prot
Match: P05659 (Myosin-2 heavy chain, non muscle OS=Acanthamoeba castellanii OX=5755 PE=3 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 6.0e-05
Identity = 77/331 (23.26%), Postives = 151/331 (45.62%), Query Frame = 0

Query: 192  QRHILRMLEKSLARELDLEKKLSESKQREEELRMKLHYTEQVALRMEEAAEVVWGRFLEA 251
            QR+  + ++    +  DLEK+L+  K    +L  +    E+ A ++E+    +  + L+ 
Sbjct: 851  QRNFQKEIDDLKKQVKDLEKELAALKDANAKLDKEKQLAEEDADKLEKDLAALKLKILD- 910

Query: 252  DNSVEILMGISKELVGRLQLVQFNLHGSFQRENEVKTKCQDWTEQLKAKEVAIQKLEKRN 311
                  L G   +L     L+Q  + G  +   E  +   D  EQ +       KLE   
Sbjct: 911  ------LEGEKADLEEDNALLQKKVAGLEEELQEETSASNDILEQKR-------KLEAEK 970

Query: 312  AELLIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEME----NLVESL 371
             EL  A   E ++ R+ ++  + +++  R +L+  Y+   A+ D L + E      +   
Sbjct: 971  GELK-ASLEEEERNRKALQEAKTKVESERNELQDKYEDEAAAHDSLKKKEEDLSRELRET 1030

Query: 372  KESICISENRAESVEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQLRQLEIQL 431
            K+++  +EN +E++ +KL   +    ++  E+  +  +    EK   SLE++L Q   QL
Sbjct: 1031 KDALADAENISETLRSKLKNTERGADDVRNELDDVTATKLQLEKTKKSLEEELAQTRAQL 1090

Query: 432  QHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIILSE------ 491
            +  KS  EA+  +   L   + D  + ++ LKSK+S AE    +A++    L E      
Sbjct: 1091 EEEKSGKEAASSKAKQLGQQLEDARSEVDSLKSKLSAAEKSLKTAKDQNRDLDEQLEDER 1150

Query: 492  -TNFELNKELTSLKARVEFLEKALDQANGEK 512
                 ++K+  +L+A++  LE  +   +G+K
Sbjct: 1151 TVRANVDKQKKALEAKLTELEDQVTALDGQK 1166

BLAST of Bhi12G000923 vs. ExPASy Swiss-Prot
Match: Q14203 (Dynactin subunit 1 OS=Homo sapiens OX=9606 GN=DCTN1 PE=1 SV=3)

HSP 1 Score: 50.4 bits (119), Expect = 7.8e-05
Identity = 78/339 (23.01%), Postives = 153/339 (45.13%), Query Frame = 0

Query: 281 QRENEVKTKCQDWTEQLKA-------KEVAIQKLEKRNAELLIAKNAELDKLREEVKSLE 340
           + E  ++ + +D  E+L+         +  +++LEK   +L   +  +  K++E+   L+
Sbjct: 215 KEEEGLRAQVRDLEEKLETLRLKRAEDKAKLKELEKHKIQLEQVQEWK-SKMQEQQADLQ 274

Query: 341 EQLKESRLDLKSAYDSNEASQDQLIEMENLVESLKESICISENRAESVEAKLTQLQETNL 400
            +LKE+R + K A ++ E   +++ +  + +E       ++E RAES++ ++  L+E   
Sbjct: 275 RRLKEARKEAKEALEAKERYMEEMADTADAIEMATLDKEMAEERAESLQQEVEALKERVD 334

Query: 401 ELTEEVSFLKDSASNKEKKVGSLEKQLRQLEIQLQHAK---------SSSEASQ--EQQN 460
           ELT ++  LK     K     +   QL+QLE Q    K         SSSE  +  + Q 
Sbjct: 335 ELTTDLEILKAEIEEKGSDGAASSYQLKQLEEQNARLKDALVRMRDLSSSEKQEHVKLQK 394

Query: 461 MLYSAIWDMETL---IEDLKSKVSKAESKTD----------SAEEHCIILSETNFELNKE 520
           ++     ++E +    E L+ ++S+AES  D           AEE   +L++ N  L ++
Sbjct: 395 LMEKKNQELEVVRQQRERLQEELSQAESTIDELKEQVDAALGAEEMVEMLTDRNLNLEEK 454

Query: 521 LTSLKARVEFLEKALDQANGEKYTNANKVNLSSKFIMDMVLQLAVERDRIQSQLSILTND 580
           +  L+  V  LE A+++ N E   NA +  L  +  +DM      E  +          D
Sbjct: 455 VRELRETVGDLE-AMNEMNDELQENARETELELREQLDMAGARVREAQKRVEAAQETVAD 514

Query: 581 NKALIEKLRTVRDGASIITRD-----RGDYDKKERSAPE 584
            +  I+K R +      + R+         +++++  PE
Sbjct: 515 YQQTIKKYRQLTAHLQDVNRELTNQQEASVERQQQPPPE 551

BLAST of Bhi12G000923 vs. ExPASy Swiss-Prot
Match: O08788 (Dynactin subunit 1 OS=Mus musculus OX=10090 GN=Dctn1 PE=1 SV=3)

HSP 1 Score: 50.4 bits (119), Expect = 7.8e-05
Identity = 78/339 (23.01%), Postives = 153/339 (45.13%), Query Frame = 0

Query: 281 QRENEVKTKCQDWTEQLKA-------KEVAIQKLEKRNAELLIAKNAELDKLREEVKSLE 340
           + E  ++ + +D  E+L+         +  +++LEK   +L   +  +  K++E+   L+
Sbjct: 215 KEEEGLRAQVRDLEEKLETLRLKRSEDKAKLKELEKHKIQLEQVQEWK-SKMQEQQADLQ 274

Query: 341 EQLKESRLDLKSAYDSNEASQDQLIEMENLVESLKESICISENRAESVEAKLTQLQETNL 400
            +LKE+R + K A ++ E   +++ +  + +E       ++E RAES++ ++  L+E   
Sbjct: 275 RRLKEARKEAKEALEAKERYMEEMADTADAIEMATLDKEMAEERAESLQQEVEALKERVD 334

Query: 401 ELTEEVSFLKDSASNKEKKVGSLEKQLRQLEIQLQHAK---------SSSEASQ--EQQN 460
           ELT ++  LK     K     +   QL+QLE Q    K         SSSE  +  + Q 
Sbjct: 335 ELTTDLEILKAEIEEKGSDGAASSYQLKQLEEQNARLKDALVRMRDLSSSEKQEHVKLQK 394

Query: 461 MLYSAIWDMETL---IEDLKSKVSKAESKTD----------SAEEHCIILSETNFELNKE 520
           ++     ++E +    E L+ ++S+AES  D           AEE   +L++ N  L ++
Sbjct: 395 LMEKKNQELEVVRQQRERLQEELSQAESTIDELKEQVDAALGAEEMVEMLTDRNLNLEEK 454

Query: 521 LTSLKARVEFLEKALDQANGEKYTNANKVNLSSKFIMDMVLQLAVERDRIQSQLSILTND 580
           +  L+  V  LE A+++ N E   NA +  L  +  +DM      E  +          D
Sbjct: 455 VRELRETVGDLE-AMNEMNDELQENARETELELREQLDMAGARVREAQKRVEAAQETVAD 514

Query: 581 NKALIEKLRTVRDGASIITRD-----RGDYDKKERSAPE 584
            +  I+K R +      + R+         +++++  PE
Sbjct: 515 YQQTIKKYRQLTAHLQDVNRELTNQQEASVERQQQPPPE 551

BLAST of Bhi12G000923 vs. NCBI nr
Match: XP_038875156.1 (WPP domain-interacting tail-anchored protein 2-like [Benincasa hispida])

HSP 1 Score: 1078.5 bits (2788), Expect = 0.0e+00
Identity = 590/590 (100.00%), Postives = 590/590 (100.00%), Query Frame = 0

Query: 1   MGMMDENACNHESQRLEMQEMENTMKTLTKLELDLAYSSEKLMNLHVLLMCLLTQENDFE 60
           MGMMDENACNHESQRLEMQEMENTMKTLTKLELDLAYSSEKLMNLHVLLMCLLTQENDFE
Sbjct: 1   MGMMDENACNHESQRLEMQEMENTMKTLTKLELDLAYSSEKLMNLHVLLMCLLTQENDFE 60

Query: 61  AMDLENEYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMDKLEAEIVDARGMVSSCRQSSE 120
           AMDLENEYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMDKLEAEIVDARGMVSSCRQSSE
Sbjct: 61  AMDLENEYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMDKLEAEIVDARGMVSSCRQSSE 120

Query: 121 VFSVVEEKLLDSEKSLVQSQQQILEVKMQSAKLQRIVLSSGNWRLEDPVMSSQNDQVLLN 180
           VFSVVEEKLLDSEKSLVQSQQQILEVKMQSAKLQRIVLSSGNWRLEDPVMSSQNDQVLLN
Sbjct: 121 VFSVVEEKLLDSEKSLVQSQQQILEVKMQSAKLQRIVLSSGNWRLEDPVMSSQNDQVLLN 180

Query: 181 INGKSNTMTEQQRHILRMLEKSLARELDLEKKLSESKQREEELRMKLHYTEQVALRMEEA 240
           INGKSNTMTEQQRHILRMLEKSLARELDLEKKLSESKQREEELRMKLHYTEQVALRMEEA
Sbjct: 181 INGKSNTMTEQQRHILRMLEKSLARELDLEKKLSESKQREEELRMKLHYTEQVALRMEEA 240

Query: 241 AEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENEVKTKCQDWTEQLKAK 300
           AEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENEVKTKCQDWTEQLKAK
Sbjct: 241 AEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENEVKTKCQDWTEQLKAK 300

Query: 301 EVAIQKLEKRNAELLIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEM 360
           EVAIQKLEKRNAELLIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEM
Sbjct: 301 EVAIQKLEKRNAELLIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEM 360

Query: 361 ENLVESLKESICISENRAESVEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQL 420
           ENLVESLKESICISENRAESVEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQL
Sbjct: 361 ENLVESLKESICISENRAESVEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQL 420

Query: 421 RQLEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIILS 480
           RQLEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIILS
Sbjct: 421 RQLEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIILS 480

Query: 481 ETNFELNKELTSLKARVEFLEKALDQANGEKYTNANKVNLSSKFIMDMVLQLAVERDRIQ 540
           ETNFELNKELTSLKARVEFLEKALDQANGEKYTNANKVNLSSKFIMDMVLQLAVERDRIQ
Sbjct: 481 ETNFELNKELTSLKARVEFLEKALDQANGEKYTNANKVNLSSKFIMDMVLQLAVERDRIQ 540

Query: 541 SQLSILTNDNKALIEKLRTVRDGASIITRDRGDYDKKERSAPENDVNIPQ 591
           SQLSILTNDNKALIEKLRTVRDGASIITRDRGDYDKKERSAPENDVNIPQ
Sbjct: 541 SQLSILTNDNKALIEKLRTVRDGASIITRDRGDYDKKERSAPENDVNIPQ 590

BLAST of Bhi12G000923 vs. NCBI nr
Match: KAA0050245.1 (WPP domain-interacting tail-anchored protein 2-like [Cucumis melo var. makuwa] >TYJ98161.1 WPP domain-interacting tail-anchored protein 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 977.2 bits (2525), Expect = 6.1e-281
Identity = 538/586 (91.81%), Postives = 562/586 (95.90%), Query Frame = 0

Query: 1   MGMMDENACNHESQRLEMQEMENTMKTLTKLELDLAYSSEKLMNLHVLLMCLLTQENDFE 60
           MGMMDEN CN ESQRLEMQEME TMKTLTKLELDLAYSSEKLMNLHVLLMCLL QENDFE
Sbjct: 1   MGMMDENGCNRESQRLEMQEMEYTMKTLTKLELDLAYSSEKLMNLHVLLMCLLAQENDFE 60

Query: 61  AMDLENEYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMDKLEAEIVDARGMV-SSCRQSS 120
           AMDLEN+YIPDDSFGK+L FDFLSGFLDSEVRELD+FM+ LEAEIVDARGM  SSC QS+
Sbjct: 61  AMDLENDYIPDDSFGKLLVFDFLSGFLDSEVRELDTFMNTLEAEIVDARGMASSSCLQST 120

Query: 121 EVFSVVEEKLLDSEKSLVQSQQQILEVKMQSAKLQRIVLSSGNWRLEDPVMSSQNDQVLL 180
           EVFSV+E KL+DSEKSLVQS++QILEVKMQS KLQRIVLSSGNWRLEDP+MSSQNDQV  
Sbjct: 121 EVFSVLEGKLIDSEKSLVQSRKQILEVKMQSTKLQRIVLSSGNWRLEDPMMSSQNDQV-F 180

Query: 181 NINGKSNTMTEQQRHILRMLEKSLARELDLEKKLSESKQREEELRMKLHYTEQVALRMEE 240
           NINGKSNTMTEQQRHILRMLEKSLARELDLEK+LSESKQREEEL+MKLHYTEQVALRMEE
Sbjct: 181 NINGKSNTMTEQQRHILRMLEKSLARELDLEKQLSESKQREEELKMKLHYTEQVALRMEE 240

Query: 241 AAEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENEVKTKCQDWTEQLKA 300
            AEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENE+KTK QDWTEQL A
Sbjct: 241 TAEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENEIKTKFQDWTEQLNA 300

Query: 301 KEVAIQKLEKRNAELLIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIE 360
           KEVAIQKLEKRNAE LIAKNAELDKLREEVKSLEEQLKE RLDLK+AY+SNEASQDQLIE
Sbjct: 301 KEVAIQKLEKRNAE-LIAKNAELDKLREEVKSLEEQLKELRLDLKTAYESNEASQDQLIE 360

Query: 361 MENLVESLKESICISENRAESVEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQ 420
           MENLVESLKESICISENRAESVE KLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQ
Sbjct: 361 MENLVESLKESICISENRAESVETKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQ 420

Query: 421 LRQLEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIIL 480
           LR+LEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIIL
Sbjct: 421 LRELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIIL 480

Query: 481 SETNFELNKELTSLKARVEFLEKALDQANGEKYTNANKVNLSSKFIMDMVLQLAVERDRI 540
           SETNFELNKELTSLK RVEFLEKALDQANGEKY NAN++NLSSKFIMDMVLQLAVERDRI
Sbjct: 481 SETNFELNKELTSLKGRVEFLEKALDQANGEKYANANEINLSSKFIMDMVLQLAVERDRI 540

Query: 541 QSQLSILTNDNKALIEKLRTVRDGASIITRDRGDYDKKERSAPEND 586
           QSQLSILTNDNKALIEKL+ +RDGASI+TRDR DYD++ERSAP+ D
Sbjct: 541 QSQLSILTNDNKALIEKLKNIRDGASIVTRDREDYDEEERSAPKKD 584

BLAST of Bhi12G000923 vs. NCBI nr
Match: XP_008466053.1 (PREDICTED: WPP domain-interacting tail-anchored protein 2-like [Cucumis melo] >XP_008466062.1 PREDICTED: WPP domain-interacting tail-anchored protein 2-like [Cucumis melo])

HSP 1 Score: 974.9 bits (2519), Expect = 3.0e-280
Identity = 536/589 (91.00%), Postives = 563/589 (95.59%), Query Frame = 0

Query: 1   MGMMDENACNHESQRLEMQEMENTMKTLTKLELDLAYSSEKLMNLHVLLMCLLTQENDFE 60
           MGMMDEN CN ESQRLEMQEME TMKTLTKLELDLAYSSEKLMNLHVLLMCLL QENDFE
Sbjct: 1   MGMMDENGCNRESQRLEMQEMEYTMKTLTKLELDLAYSSEKLMNLHVLLMCLLAQENDFE 60

Query: 61  AMDLENEYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMDKLEAEIVDARGMV-SSCRQSS 120
           AMDLEN+YIPDDSFGK+L FDFLSGFLDSEVRELD+FM+ LEAEIVDARGM  SSC QS+
Sbjct: 61  AMDLENDYIPDDSFGKLLVFDFLSGFLDSEVRELDTFMNTLEAEIVDARGMASSSCLQST 120

Query: 121 EVFSVVEEKLLDSEKSLVQSQQQILEVKMQSAKLQRIVLSSGNWRLEDPVMSSQNDQVLL 180
           EVFSV+E KL+DSEKSLVQS++QILEVKMQS KLQRIVLS GNWRLEDP+MSSQNDQV  
Sbjct: 121 EVFSVLEGKLIDSEKSLVQSRKQILEVKMQSTKLQRIVLSRGNWRLEDPMMSSQNDQV-F 180

Query: 181 NINGKSNTMTEQQRHILRMLEKSLARELDLEKKLSESKQREEELRMKLHYTEQVALRMEE 240
           NINGKSNTMTEQQRHILRMLEKSLARELDLEK+LSESKQREEEL+MKLHYTEQVALRMEE
Sbjct: 181 NINGKSNTMTEQQRHILRMLEKSLARELDLEKQLSESKQREEELKMKLHYTEQVALRMEE 240

Query: 241 AAEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENEVKTKCQDWTEQLKA 300
            AEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRE+E+KTK QDWTEQL A
Sbjct: 241 TAEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQREHEIKTKFQDWTEQLNA 300

Query: 301 KEVAIQKLEKRNAELLIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIE 360
           KEVAIQKLEKRNAE LIAKNAELDKLREEVKSLEEQLKE RLDLK+AY+SNEASQDQLIE
Sbjct: 301 KEVAIQKLEKRNAE-LIAKNAELDKLREEVKSLEEQLKELRLDLKTAYESNEASQDQLIE 360

Query: 361 MENLVESLKESICISENRAESVEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQ 420
           MENLVESLKESICISENRAESVE KLTQLQETNLELTEEVSFLKDSAS+KEKKVGSLEKQ
Sbjct: 361 MENLVESLKESICISENRAESVETKLTQLQETNLELTEEVSFLKDSASSKEKKVGSLEKQ 420

Query: 421 LRQLEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIIL 480
           LR+LEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIIL
Sbjct: 421 LRELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIIL 480

Query: 481 SETNFELNKELTSLKARVEFLEKALDQANGEKYTNANKVNLSSKFIMDMVLQLAVERDRI 540
           SETNFELNKELTSLK RVEFLEKALDQANGEKY NAN++NLSSKFIMDMVLQLAVERDR+
Sbjct: 481 SETNFELNKELTSLKGRVEFLEKALDQANGEKYANANEINLSSKFIMDMVLQLAVERDRV 540

Query: 541 QSQLSILTNDNKALIEKLRTVRDGASIITRDRGDYDKKERSAPENDVNI 589
           Q QLSILTNDNKALIEKL+ +RDGASI+TRDR DYD++ERSAP+ DVNI
Sbjct: 541 QRQLSILTNDNKALIEKLKNIRDGASIVTRDREDYDEEERSAPKKDVNI 587

BLAST of Bhi12G000923 vs. NCBI nr
Match: XP_004149502.1 (WPP domain-interacting tail-anchored protein 2 [Cucumis sativus] >XP_011654564.1 WPP domain-interacting tail-anchored protein 2 [Cucumis sativus] >XP_031741688.1 WPP domain-interacting tail-anchored protein 2 [Cucumis sativus] >KGN49805.1 hypothetical protein Csa_004672 [Cucumis sativus])

HSP 1 Score: 964.9 bits (2493), Expect = 3.1e-277
Identity = 530/588 (90.14%), Postives = 556/588 (94.56%), Query Frame = 0

Query: 1   MGMMDENACNHESQRLEMQEMENTMKTLTKLELDLAYSSEKLMNLHVLLMCLLTQENDFE 60
           MGMMDEN C+ ESQRLEMQEME TMKTLTKLELDLAYSSEKLMNLHVLLMCLL QENDFE
Sbjct: 1   MGMMDENVCHRESQRLEMQEMEYTMKTLTKLELDLAYSSEKLMNLHVLLMCLLAQENDFE 60

Query: 61  AMDLENEYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMDKLEAEIVDARGMVSSCRQSSE 120
           AMDL ++YIPDDSFGKVL FDFLSGFLDSEVRELDSFM+ LEAE VDARGMVS+C QS+E
Sbjct: 61  AMDLVHDYIPDDSFGKVLVFDFLSGFLDSEVRELDSFMNTLEAETVDARGMVSTCLQSTE 120

Query: 121 VFSVVEEKLLDSEKSLVQSQQQILEVKMQSAKLQRIVLSSGNWRLEDPVMSSQNDQVLLN 180
           VFSV+E KLLDSEKSLVQS++QILEVKMQS KLQRIVLSSGNWRLEDP+MSSQNDQV  N
Sbjct: 121 VFSVLEGKLLDSEKSLVQSRKQILEVKMQSTKLQRIVLSSGNWRLEDPMMSSQNDQV-FN 180

Query: 181 INGKSNTMTEQQRHILRMLEKSLARELDLEKKLSESKQREEELRMKLHYTEQVALRMEEA 240
           INGKSNTMTEQQRHILRMLEKSLARELDLEK+LSESKQREEEL+MKLHYTEQVALRMEE 
Sbjct: 181 INGKSNTMTEQQRHILRMLEKSLARELDLEKQLSESKQREEELKMKLHYTEQVALRMEET 240

Query: 241 AEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENEVKTKCQDWTEQLKAK 300
           AEVVWGRFLEADNSVEILMGISKE VGRLQLVQFNLHGSFQREN++K K QDWTEQL AK
Sbjct: 241 AEVVWGRFLEADNSVEILMGISKEFVGRLQLVQFNLHGSFQRENDIKAKFQDWTEQLNAK 300

Query: 301 EVAIQKLEKRNAELLIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEM 360
           EVAIQKLEKRNAE LIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEM
Sbjct: 301 EVAIQKLEKRNAE-LIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEM 360

Query: 361 ENLVESLKESICISENRAESVEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQL 420
           ENLVE+LKESICISENRAE  E KLTQLQETNLELTEEVSFLKDS SNKEKKVGSLEKQL
Sbjct: 361 ENLVETLKESICISENRAEGAETKLTQLQETNLELTEEVSFLKDSVSNKEKKVGSLEKQL 420

Query: 421 RQLEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIILS 480
           R+LEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIILS
Sbjct: 421 RELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIILS 480

Query: 481 ETNFELNKELTSLKARVEFLEKALDQANGEKYTNANKVNLSSKFIMDMVLQLAVERDRIQ 540
           ETNFELNKELTSLK +VEFLEK+LDQANGEKY NAN++NLSSKF+MDMVLQLAVERDRIQ
Sbjct: 481 ETNFELNKELTSLKGQVEFLEKSLDQANGEKYANANEINLSSKFVMDMVLQLAVERDRIQ 540

Query: 541 SQLSILTNDNKALIEKLRTVRDGASIITRDRGDYDKKERSAPENDVNI 589
           SQLSILTNDNKALIEKL+ VRD A I+T  R DYD+KE+SAP+ DVNI
Sbjct: 541 SQLSILTNDNKALIEKLKNVRDSAPIVTLHREDYDEKEQSAPKKDVNI 586

BLAST of Bhi12G000923 vs. NCBI nr
Match: KAG7016657.1 (WPP domain-interacting tail-anchored protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 958.4 bits (2476), Expect = 2.9e-275
Identity = 522/588 (88.78%), Postives = 562/588 (95.58%), Query Frame = 0

Query: 1   MGMMDENACNHESQRLEMQEMENTMKTLTKLELDLAYSSEKLMNLHVLLMCLLTQENDFE 60
           MGMMDENACN ESQ LEMQE+E+TMKTLTKLEL+LAYSSEKL NLHVLLMCL+ QENDFE
Sbjct: 1   MGMMDENACNQESQGLEMQELESTMKTLTKLELNLAYSSEKLTNLHVLLMCLMAQENDFE 60

Query: 61  AMDLENEYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMDKLEAEIVDARGMVSSCRQSSE 120
            +DLEN+ IPDD F KVLAFDFLSGFLDSEVRELDS MD LEAEIVD+RGM+SSCRQSSE
Sbjct: 61  GVDLENDSIPDDLFEKVLAFDFLSGFLDSEVRELDSNMDSLEAEIVDSRGMISSCRQSSE 120

Query: 121 VFSVVEEKLLDSEKSLVQSQQQILEVKMQSAKLQRIVLSSGNWRLEDPVMSSQNDQVLLN 180
           VF+VVEEKL DSE+S+VQS+QQILEVKMQ AKLQR+VLSSGNWRLEDPVMSSQNDQV LN
Sbjct: 121 VFNVVEEKLNDSERSIVQSRQQILEVKMQLAKLQRMVLSSGNWRLEDPVMSSQNDQV-LN 180

Query: 181 INGKSNTMTEQQRHILRMLEKSLARELDLEKKLSESKQREEELRMKLHYTEQVALRMEEA 240
           INGKSN +TEQQRHILRMLEKSLARELDLEKKLSESKQREEELR+KLHYTEQV LRMEEA
Sbjct: 181 INGKSNPITEQQRHILRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVTLRMEEA 240

Query: 241 AEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENEVKTKCQDWTEQLKAK 300
           AEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENE+K K QDWTEQLKAK
Sbjct: 241 AEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWTEQLKAK 300

Query: 301 EVAIQKLEKRNAELLIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEM 360
           EV+IQKLEKRNA+ LIAKNAELDKLREEVKSLE+Q+KES+LDLKSAYDSNEASQDQLIEM
Sbjct: 301 EVSIQKLEKRNAD-LIAKNAELDKLREEVKSLEDQVKESKLDLKSAYDSNEASQDQLIEM 360

Query: 361 ENLVESLKESICISENRAESVEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQL 420
           ENL+ESLKESICISENRAE+VEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQL
Sbjct: 361 ENLIESLKESICISENRAENVEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQL 420

Query: 421 RQLEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIILS 480
           R+LEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIE+LKSKVSKAESKTDSAEEHCIILS
Sbjct: 421 RELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEELKSKVSKAESKTDSAEEHCIILS 480

Query: 481 ETNFELNKELTSLKARVEFLEKALDQANGEKYTNANKVNLSSKFIMDMVLQLAVERDRIQ 540
           ETNFELNKEL SLK R+EFLEKALDQA   K+TNAN++NLS+KFIMDMVLQLAVERDRIQ
Sbjct: 481 ETNFELNKELASLKTRIEFLEKALDQARSAKFTNANEINLSTKFIMDMVLQLAVERDRIQ 540

Query: 541 SQLSILTNDNKALIEKLRTVRDGASIITRDRGDYDKKERSAPENDVNI 589
           SQLS+LTNDNKALIE+L+ V++GAS++TRDRGDYD+++RSAP  DVNI
Sbjct: 541 SQLSLLTNDNKALIERLKNVKNGASVVTRDRGDYDEEQRSAPMKDVNI 586

BLAST of Bhi12G000923 vs. ExPASy TrEMBL
Match: A0A5A7U9G6 (WPP domain-interacting tail-anchored protein 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00430 PE=4 SV=1)

HSP 1 Score: 977.2 bits (2525), Expect = 2.9e-281
Identity = 538/586 (91.81%), Postives = 562/586 (95.90%), Query Frame = 0

Query: 1   MGMMDENACNHESQRLEMQEMENTMKTLTKLELDLAYSSEKLMNLHVLLMCLLTQENDFE 60
           MGMMDEN CN ESQRLEMQEME TMKTLTKLELDLAYSSEKLMNLHVLLMCLL QENDFE
Sbjct: 1   MGMMDENGCNRESQRLEMQEMEYTMKTLTKLELDLAYSSEKLMNLHVLLMCLLAQENDFE 60

Query: 61  AMDLENEYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMDKLEAEIVDARGMV-SSCRQSS 120
           AMDLEN+YIPDDSFGK+L FDFLSGFLDSEVRELD+FM+ LEAEIVDARGM  SSC QS+
Sbjct: 61  AMDLENDYIPDDSFGKLLVFDFLSGFLDSEVRELDTFMNTLEAEIVDARGMASSSCLQST 120

Query: 121 EVFSVVEEKLLDSEKSLVQSQQQILEVKMQSAKLQRIVLSSGNWRLEDPVMSSQNDQVLL 180
           EVFSV+E KL+DSEKSLVQS++QILEVKMQS KLQRIVLSSGNWRLEDP+MSSQNDQV  
Sbjct: 121 EVFSVLEGKLIDSEKSLVQSRKQILEVKMQSTKLQRIVLSSGNWRLEDPMMSSQNDQV-F 180

Query: 181 NINGKSNTMTEQQRHILRMLEKSLARELDLEKKLSESKQREEELRMKLHYTEQVALRMEE 240
           NINGKSNTMTEQQRHILRMLEKSLARELDLEK+LSESKQREEEL+MKLHYTEQVALRMEE
Sbjct: 181 NINGKSNTMTEQQRHILRMLEKSLARELDLEKQLSESKQREEELKMKLHYTEQVALRMEE 240

Query: 241 AAEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENEVKTKCQDWTEQLKA 300
            AEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENE+KTK QDWTEQL A
Sbjct: 241 TAEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENEIKTKFQDWTEQLNA 300

Query: 301 KEVAIQKLEKRNAELLIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIE 360
           KEVAIQKLEKRNAE LIAKNAELDKLREEVKSLEEQLKE RLDLK+AY+SNEASQDQLIE
Sbjct: 301 KEVAIQKLEKRNAE-LIAKNAELDKLREEVKSLEEQLKELRLDLKTAYESNEASQDQLIE 360

Query: 361 MENLVESLKESICISENRAESVEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQ 420
           MENLVESLKESICISENRAESVE KLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQ
Sbjct: 361 MENLVESLKESICISENRAESVETKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQ 420

Query: 421 LRQLEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIIL 480
           LR+LEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIIL
Sbjct: 421 LRELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIIL 480

Query: 481 SETNFELNKELTSLKARVEFLEKALDQANGEKYTNANKVNLSSKFIMDMVLQLAVERDRI 540
           SETNFELNKELTSLK RVEFLEKALDQANGEKY NAN++NLSSKFIMDMVLQLAVERDRI
Sbjct: 481 SETNFELNKELTSLKGRVEFLEKALDQANGEKYANANEINLSSKFIMDMVLQLAVERDRI 540

Query: 541 QSQLSILTNDNKALIEKLRTVRDGASIITRDRGDYDKKERSAPEND 586
           QSQLSILTNDNKALIEKL+ +RDGASI+TRDR DYD++ERSAP+ D
Sbjct: 541 QSQLSILTNDNKALIEKLKNIRDGASIVTRDREDYDEEERSAPKKD 584

BLAST of Bhi12G000923 vs. ExPASy TrEMBL
Match: A0A1S3CRS0 (WPP domain-interacting tail-anchored protein 2-like OS=Cucumis melo OX=3656 GN=LOC103503596 PE=4 SV=1)

HSP 1 Score: 974.9 bits (2519), Expect = 1.5e-280
Identity = 536/589 (91.00%), Postives = 563/589 (95.59%), Query Frame = 0

Query: 1   MGMMDENACNHESQRLEMQEMENTMKTLTKLELDLAYSSEKLMNLHVLLMCLLTQENDFE 60
           MGMMDEN CN ESQRLEMQEME TMKTLTKLELDLAYSSEKLMNLHVLLMCLL QENDFE
Sbjct: 1   MGMMDENGCNRESQRLEMQEMEYTMKTLTKLELDLAYSSEKLMNLHVLLMCLLAQENDFE 60

Query: 61  AMDLENEYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMDKLEAEIVDARGMV-SSCRQSS 120
           AMDLEN+YIPDDSFGK+L FDFLSGFLDSEVRELD+FM+ LEAEIVDARGM  SSC QS+
Sbjct: 61  AMDLENDYIPDDSFGKLLVFDFLSGFLDSEVRELDTFMNTLEAEIVDARGMASSSCLQST 120

Query: 121 EVFSVVEEKLLDSEKSLVQSQQQILEVKMQSAKLQRIVLSSGNWRLEDPVMSSQNDQVLL 180
           EVFSV+E KL+DSEKSLVQS++QILEVKMQS KLQRIVLS GNWRLEDP+MSSQNDQV  
Sbjct: 121 EVFSVLEGKLIDSEKSLVQSRKQILEVKMQSTKLQRIVLSRGNWRLEDPMMSSQNDQV-F 180

Query: 181 NINGKSNTMTEQQRHILRMLEKSLARELDLEKKLSESKQREEELRMKLHYTEQVALRMEE 240
           NINGKSNTMTEQQRHILRMLEKSLARELDLEK+LSESKQREEEL+MKLHYTEQVALRMEE
Sbjct: 181 NINGKSNTMTEQQRHILRMLEKSLARELDLEKQLSESKQREEELKMKLHYTEQVALRMEE 240

Query: 241 AAEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENEVKTKCQDWTEQLKA 300
            AEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRE+E+KTK QDWTEQL A
Sbjct: 241 TAEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQREHEIKTKFQDWTEQLNA 300

Query: 301 KEVAIQKLEKRNAELLIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIE 360
           KEVAIQKLEKRNAE LIAKNAELDKLREEVKSLEEQLKE RLDLK+AY+SNEASQDQLIE
Sbjct: 301 KEVAIQKLEKRNAE-LIAKNAELDKLREEVKSLEEQLKELRLDLKTAYESNEASQDQLIE 360

Query: 361 MENLVESLKESICISENRAESVEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQ 420
           MENLVESLKESICISENRAESVE KLTQLQETNLELTEEVSFLKDSAS+KEKKVGSLEKQ
Sbjct: 361 MENLVESLKESICISENRAESVETKLTQLQETNLELTEEVSFLKDSASSKEKKVGSLEKQ 420

Query: 421 LRQLEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIIL 480
           LR+LEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIIL
Sbjct: 421 LRELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIIL 480

Query: 481 SETNFELNKELTSLKARVEFLEKALDQANGEKYTNANKVNLSSKFIMDMVLQLAVERDRI 540
           SETNFELNKELTSLK RVEFLEKALDQANGEKY NAN++NLSSKFIMDMVLQLAVERDR+
Sbjct: 481 SETNFELNKELTSLKGRVEFLEKALDQANGEKYANANEINLSSKFIMDMVLQLAVERDRV 540

Query: 541 QSQLSILTNDNKALIEKLRTVRDGASIITRDRGDYDKKERSAPENDVNI 589
           Q QLSILTNDNKALIEKL+ +RDGASI+TRDR DYD++ERSAP+ DVNI
Sbjct: 541 QRQLSILTNDNKALIEKLKNIRDGASIVTRDREDYDEEERSAPKKDVNI 587

BLAST of Bhi12G000923 vs. ExPASy TrEMBL
Match: A0A0A0KJA1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G136890 PE=4 SV=1)

HSP 1 Score: 964.9 bits (2493), Expect = 1.5e-277
Identity = 530/588 (90.14%), Postives = 556/588 (94.56%), Query Frame = 0

Query: 1   MGMMDENACNHESQRLEMQEMENTMKTLTKLELDLAYSSEKLMNLHVLLMCLLTQENDFE 60
           MGMMDEN C+ ESQRLEMQEME TMKTLTKLELDLAYSSEKLMNLHVLLMCLL QENDFE
Sbjct: 1   MGMMDENVCHRESQRLEMQEMEYTMKTLTKLELDLAYSSEKLMNLHVLLMCLLAQENDFE 60

Query: 61  AMDLENEYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMDKLEAEIVDARGMVSSCRQSSE 120
           AMDL ++YIPDDSFGKVL FDFLSGFLDSEVRELDSFM+ LEAE VDARGMVS+C QS+E
Sbjct: 61  AMDLVHDYIPDDSFGKVLVFDFLSGFLDSEVRELDSFMNTLEAETVDARGMVSTCLQSTE 120

Query: 121 VFSVVEEKLLDSEKSLVQSQQQILEVKMQSAKLQRIVLSSGNWRLEDPVMSSQNDQVLLN 180
           VFSV+E KLLDSEKSLVQS++QILEVKMQS KLQRIVLSSGNWRLEDP+MSSQNDQV  N
Sbjct: 121 VFSVLEGKLLDSEKSLVQSRKQILEVKMQSTKLQRIVLSSGNWRLEDPMMSSQNDQV-FN 180

Query: 181 INGKSNTMTEQQRHILRMLEKSLARELDLEKKLSESKQREEELRMKLHYTEQVALRMEEA 240
           INGKSNTMTEQQRHILRMLEKSLARELDLEK+LSESKQREEEL+MKLHYTEQVALRMEE 
Sbjct: 181 INGKSNTMTEQQRHILRMLEKSLARELDLEKQLSESKQREEELKMKLHYTEQVALRMEET 240

Query: 241 AEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENEVKTKCQDWTEQLKAK 300
           AEVVWGRFLEADNSVEILMGISKE VGRLQLVQFNLHGSFQREN++K K QDWTEQL AK
Sbjct: 241 AEVVWGRFLEADNSVEILMGISKEFVGRLQLVQFNLHGSFQRENDIKAKFQDWTEQLNAK 300

Query: 301 EVAIQKLEKRNAELLIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEM 360
           EVAIQKLEKRNAE LIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEM
Sbjct: 301 EVAIQKLEKRNAE-LIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEM 360

Query: 361 ENLVESLKESICISENRAESVEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQL 420
           ENLVE+LKESICISENRAE  E KLTQLQETNLELTEEVSFLKDS SNKEKKVGSLEKQL
Sbjct: 361 ENLVETLKESICISENRAEGAETKLTQLQETNLELTEEVSFLKDSVSNKEKKVGSLEKQL 420

Query: 421 RQLEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIILS 480
           R+LEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIILS
Sbjct: 421 RELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIILS 480

Query: 481 ETNFELNKELTSLKARVEFLEKALDQANGEKYTNANKVNLSSKFIMDMVLQLAVERDRIQ 540
           ETNFELNKELTSLK +VEFLEK+LDQANGEKY NAN++NLSSKF+MDMVLQLAVERDRIQ
Sbjct: 481 ETNFELNKELTSLKGQVEFLEKSLDQANGEKYANANEINLSSKFVMDMVLQLAVERDRIQ 540

Query: 541 SQLSILTNDNKALIEKLRTVRDGASIITRDRGDYDKKERSAPENDVNI 589
           SQLSILTNDNKALIEKL+ VRD A I+T  R DYD+KE+SAP+ DVNI
Sbjct: 541 SQLSILTNDNKALIEKLKNVRDSAPIVTLHREDYDEKEQSAPKKDVNI 586

BLAST of Bhi12G000923 vs. ExPASy TrEMBL
Match: A0A6J1K445 (WPP domain-interacting tail-anchored protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111489874 PE=4 SV=1)

HSP 1 Score: 953.7 bits (2464), Expect = 3.5e-274
Identity = 521/588 (88.61%), Postives = 560/588 (95.24%), Query Frame = 0

Query: 1   MGMMDENACNHESQRLEMQEMENTMKTLTKLELDLAYSSEKLMNLHVLLMCLLTQENDFE 60
           MGMMDENACN E Q LEMQE+E++MKTLTKLEL+LAYSSEKL NLHVLLMCL+ QENDFE
Sbjct: 1   MGMMDENACNQEYQGLEMQELESSMKTLTKLELNLAYSSEKLTNLHVLLMCLMAQENDFE 60

Query: 61  AMDLENEYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMDKLEAEIVDARGMVSSCRQSSE 120
            +DLEN+ IPDD F KVLAFDFLSGFLDSEVRELDS MD LEAEIVD+RGM+SSCRQSSE
Sbjct: 61  GVDLENDSIPDDLFEKVLAFDFLSGFLDSEVRELDSNMDSLEAEIVDSRGMISSCRQSSE 120

Query: 121 VFSVVEEKLLDSEKSLVQSQQQILEVKMQSAKLQRIVLSSGNWRLEDPVMSSQNDQVLLN 180
           VF+VVEEKL DSE+S+VQS+QQILEVKMQ AKLQR+VLSSGNWRLEDPVMSSQNDQV LN
Sbjct: 121 VFNVVEEKLNDSERSIVQSRQQILEVKMQLAKLQRMVLSSGNWRLEDPVMSSQNDQV-LN 180

Query: 181 INGKSNTMTEQQRHILRMLEKSLARELDLEKKLSESKQREEELRMKLHYTEQVALRMEEA 240
           INGKSN +TEQQRHILRMLEKSLARELDLEKKLSESKQREEELR+KLHYTEQV LRMEEA
Sbjct: 181 INGKSNPITEQQRHILRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVTLRMEEA 240

Query: 241 AEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENEVKTKCQDWTEQLKAK 300
           AEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENE+K K QDW EQLKAK
Sbjct: 241 AEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWMEQLKAK 300

Query: 301 EVAIQKLEKRNAELLIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEM 360
           EV+IQKLEKRNA+ LIAKNAELDKLREEVKSLEEQLKES+LDLKS YDSNEASQDQLIEM
Sbjct: 301 EVSIQKLEKRNAD-LIAKNAELDKLREEVKSLEEQLKESKLDLKSVYDSNEASQDQLIEM 360

Query: 361 ENLVESLKESICISENRAESVEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQL 420
           ENL+ESLKESICISENRAE+VEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQL
Sbjct: 361 ENLIESLKESICISENRAENVEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQL 420

Query: 421 RQLEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIILS 480
           R+LEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIE+LKSKVSKAESKTDSAEEHCIILS
Sbjct: 421 RELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEELKSKVSKAESKTDSAEEHCIILS 480

Query: 481 ETNFELNKELTSLKARVEFLEKALDQANGEKYTNANKVNLSSKFIMDMVLQLAVERDRIQ 540
           ETNFELNKEL SLKAR+EFLEKALDQA   K+TNAN++NLS+KFIMDMVLQLAVERDRIQ
Sbjct: 481 ETNFELNKELASLKARIEFLEKALDQARSAKFTNANEINLSTKFIMDMVLQLAVERDRIQ 540

Query: 541 SQLSILTNDNKALIEKLRTVRDGASIITRDRGDYDKKERSAPENDVNI 589
           SQLSILTNDNKALIE+L+ V++GAS++TRDRG+YD+++RSAP  DVNI
Sbjct: 541 SQLSILTNDNKALIERLKNVKNGASVVTRDRGEYDEEQRSAPMKDVNI 586

BLAST of Bhi12G000923 vs. ExPASy TrEMBL
Match: A0A6J1FMT1 (WPP domain-interacting tail-anchored protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111445366 PE=4 SV=1)

HSP 1 Score: 951.8 bits (2459), Expect = 1.3e-273
Identity = 521/588 (88.61%), Postives = 560/588 (95.24%), Query Frame = 0

Query: 1   MGMMDENACNHESQRLEMQEMENTMKTLTKLELDLAYSSEKLMNLHVLLMCLLTQENDFE 60
           MGMMDENACN ESQ LEMQE+E+TMKTLTKLEL+LAYSSEKL NLHVLLMCL+ QENDFE
Sbjct: 1   MGMMDENACNQESQGLEMQELESTMKTLTKLELNLAYSSEKLTNLHVLLMCLMAQENDFE 60

Query: 61  AMDLENEYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMDKLEAEIVDARGMVSSCRQSSE 120
            +DLEN+ IPDD F KVLAFDFLSGFLDSEVRELDS MD LEAEIVD+RGM SSCRQSSE
Sbjct: 61  GVDLENDSIPDDLFEKVLAFDFLSGFLDSEVRELDSNMDSLEAEIVDSRGMTSSCRQSSE 120

Query: 121 VFSVVEEKLLDSEKSLVQSQQQILEVKMQSAKLQRIVLSSGNWRLEDPVMSSQNDQVLLN 180
           VF+VVEEKL DSE+S+VQS+QQILEVKMQ AKLQR+VLSSGNWRLEDPVMS QNDQV LN
Sbjct: 121 VFNVVEEKLNDSERSIVQSRQQILEVKMQLAKLQRMVLSSGNWRLEDPVMSYQNDQV-LN 180

Query: 181 INGKSNTMTEQQRHILRMLEKSLARELDLEKKLSESKQREEELRMKLHYTEQVALRMEEA 240
           INGKSN +TEQQRHILRMLEKSLARELDLEKKLSESKQREEELR+KLHYTEQV LRMEEA
Sbjct: 181 INGKSNPITEQQRHILRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVTLRMEEA 240

Query: 241 AEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENEVKTKCQDWTEQLKAK 300
           AEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENE+K K QDWTEQLKAK
Sbjct: 241 AEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENEIKDKFQDWTEQLKAK 300

Query: 301 EVAIQKLEKRNAELLIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEM 360
           EV+IQKLEKRNA+ LIAKNAELDKLREEVKSLE+Q+KES+LDLKSAYDSNEASQDQLIEM
Sbjct: 301 EVSIQKLEKRNAD-LIAKNAELDKLREEVKSLEDQVKESKLDLKSAYDSNEASQDQLIEM 360

Query: 361 ENLVESLKESICISENRAESVEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQL 420
           ENL+ESLKESICISENRAE+VEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQL
Sbjct: 361 ENLIESLKESICISENRAENVEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQL 420

Query: 421 RQLEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIILS 480
           R+LEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIE+LKSKVSKAESKTDSAEEH IILS
Sbjct: 421 RELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEELKSKVSKAESKTDSAEEHYIILS 480

Query: 481 ETNFELNKELTSLKARVEFLEKALDQANGEKYTNANKVNLSSKFIMDMVLQLAVERDRIQ 540
           ETNFELNKEL SLKAR+EFLEKALDQA   K+TNAN++NLS+KFIMDMVLQLAVERDRIQ
Sbjct: 481 ETNFELNKELASLKARIEFLEKALDQARSAKFTNANEINLSTKFIMDMVLQLAVERDRIQ 540

Query: 541 SQLSILTNDNKALIEKLRTVRDGASIITRDRGDYDKKERSAPENDVNI 589
           SQLS+LTNDNKALIE+L+ V++GAS++TRDRGDYD+++RSAP  DVNI
Sbjct: 541 SQLSLLTNDNKALIERLKNVKNGASVVTRDRGDYDEEQRSAPMKDVNI 586