Bhi12G000902 (gene) Wax gourd (B227) v1

Overview
NameBhi12G000902
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionPectinesterase
Locationchr12: 31276980 .. 31279293 (+)
RNA-Seq ExpressionBhi12G000902
SyntenyBhi12G000902
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTCCATTCAATATCATTACAAAGACCTCCAAAAAAAAGAAAAGAAAAGTACTTCCTGCACAAAACCCACTTTCTTTTTCCCATCTCTATGGTGGGAAAACACTTTTCATCTGGGTTTTGATCATTCTCATTTGCAGGAGGAAAACTTTTCATATAAGAGGGACAAAAAATAAAGAAACAAAATGGTGAATAAGGTGGTCTTGCCTGGAATATCCCTTATTCTTGTTGTTGGTGTAGCACTTGCAGTTGTTGCAGTGGTTAACAAGAACAATAATTCCACGACGGAGAATCTGTCCCCAAAGATGAAGGCAGTCTCAACCATTTGTTCTAATACTAATTACCAAGAAGAATGCCAAAATACACTTACCAATGTTGCTCATAATGCCTCTTCAGATGACCCTAAGGAGTATGTTAAAGCAGCAATTCTTGCCACTATGGAGGAGGTTAAAAAGGGCTATAATTTGACTGATGGCTTTATGATTGAGGCTGCTAACAATGCCAGTATCAAAATGGGTGTTGAGGATTGCAAGGACTTATTGCAATTCGCCATTGCCCAGTTGCAGGCATCTTACACAACTGTTGGCGAACCTGATTTGCACACCAACGCCGATCGTGTCGCTGATATTAAGAATTGGCTCACTGCTGTTATTTCTTATCAACAATCTTGTCTCGATGGACTTGGAGAATTTGATCCAAACCTTCAACAGAAGATGCAAGATGGTCTTGATATTGCTGCTAAGCTTACTAGTAATGCTCTTGCTATTGTTGATACAGTTTCTGATATTCTTCAAAACTTCGGCTTGCAATTCAAGGTAATTAAGGACAATGATTAGATGGAGACTAACCCACGTTCCCATTTTTTTTACACACTAAAATAAAGTTTTTTGACAGGTTCAACCAAGTGGTCGCAAGCTCCTTGGAACAATAGAGGTTGGGAGCGACGGGTTCCCGACATGGATGTCGGGGGCAGACAGGAAGTTGCTGGCATCAAGAGGAGGCGGTCGAGTGAAACCGAATGCAGTGGTGGCGAAGGATGGTAGCGGACAGTTCAAGACCATTGGTGCTGCATTGGCTGCATACCCAAAGGCCCTAAAAGGAAGATATGTAATCTACGTGAAAGCAGGGATCTATAATGAATATGTCACTGTGACTAAAGACATGACAAACATTTTTATGTATGGAGATGGCCCAAAAAGAACCATTGTCACTGGTCGCAGGAGCAACCGTGCTGGCTACACCACCCAAGACACTGCTACTTTTAGTAACTGCCACACTATACTTGATTTCAATCTAAGCCCTCTCCTTATTTTCTCCCTCTAAATCTCTTTGTCTTAATGTAAAATTATCCTTTTATCTCTGAGACCTCTAAATCTATAACAACTTACTCATATTTATCTCTTGCAGTCGCCATAGGAGCAGGATTTCTTTGCAAATCCATGGGATTCCAAAACACAGCCGGTCCTGAAGGCCACCAAGCCGTAGCCCTTAGAGTCCAATCCGACAAATCTGCCTTCTTTAACTGTAGAATGGATGGCTACCAAGACACTCTCTACGTGCAAACTGAACGTCAATTCTACCGCAATTGTGTCATATCCGGCACCGTAGATTTCATCTTCGGCGACTCAACCACCATCATTCAAAACTCCTTGATCATCGTTCGTCGTCCAATGGACAACCAACAAAACACTGTCACAGCCAATGGTCGTGCCGATCCAAAAGAAGTATCAGGCTTGGTGATCCACAACTGCAGAATTGTGCCAGAACAGAAGCTCTTTGCTGAGAGGTTCAAGATTCCAACATACTTGGGGCGCCCATGGAAGCAATACGCTAGAACTGTGATCATGGAGACTACATTGGGCGACTTCATTAAGCCGGATGGATACATGCCATGGTCTGGAAATTTTGCATTGGAGACTTGTTTGTATTTGGAGTATGGGAACAGAGGACCTGGTGCTAACACCAACAGGAGAGTAAGATGGAAGGGTGTTAAAGTCATTCCAAGAACTGAAGCTTTGAAATTCACTGCTGGATCATTTCTTCAAGGAGGAAGTTGGCTTCCAGGCACTGGTGGGCCTTACCTCTTGGGCTTGAAAGGCTAAAAAAATAAGGGATTGTGCTTGTTGATGAAGAATGTAATATAATTTATTTGATTTCCCATTCTATAACAATTATGCCTTAGATCTTAGTTGGTATTTTGTAAGCAATTGCAAATGGCCTTAATGGTCACAACTTTGAGAAGGCATTCATTTTTTACAAGAATGTTAGCAACAAATATCGTGTTATGTTATTACGAATTTTAACCGTTGCAAAAATT

mRNA sequence

CCTCCATTCAATATCATTACAAAGACCTCCAAAAAAAAGAAAAGAAAAGTACTTCCTGCACAAAACCCACTTTCTTTTTCCCATCTCTATGGTGGGAAAACACTTTTCATCTGGGTTTTGATCATTCTCATTTGCAGGAGGAAAACTTTTCATATAAGAGGGACAAAAAATAAAGAAACAAAATGGTGAATAAGGTGGTCTTGCCTGGAATATCCCTTATTCTTGTTGTTGGTGTAGCACTTGCAGTTGTTGCAGTGGTTAACAAGAACAATAATTCCACGACGGAGAATCTGTCCCCAAAGATGAAGGCAGTCTCAACCATTTGTTCTAATACTAATTACCAAGAAGAATGCCAAAATACACTTACCAATGTTGCTCATAATGCCTCTTCAGATGACCCTAAGGAGTATGTTAAAGCAGCAATTCTTGCCACTATGGAGGAGGTTAAAAAGGGCTATAATTTGACTGATGGCTTTATGATTGAGGCTGCTAACAATGCCAGTATCAAAATGGGTGTTGAGGATTGCAAGGACTTATTGCAATTCGCCATTGCCCAGTTGCAGGCATCTTACACAACTGTTGGCGAACCTGATTTGCACACCAACGCCGATCGTGTCGCTGATATTAAGAATTGGCTCACTGCTGTTATTTCTTATCAACAATCTTGTCTCGATGGACTTGGAGAATTTGATCCAAACCTTCAACAGAAGATGCAAGATGGTCTTGATATTGCTGCTAAGCTTACTAGTAATGCTCTTGCTATTGTTGATACAGTTTCTGATATTCTTCAAAACTTCGGCTTGCAATTCAAGGTTCAACCAAGTGGTCGCAAGCTCCTTGGAACAATAGAGGTTGGGAGCGACGGGTTCCCGACATGGATGTCGGGGGCAGACAGGAAGTTGCTGGCATCAAGAGGAGGCGGTCGAGTGAAACCGAATGCAGTGGTGGCGAAGGATGGTAGCGGACAGTTCAAGACCATTGGTGCTGCATTGGCTGCATACCCAAAGGCCCTAAAAGGAAGATATGTAATCTACGTGAAAGCAGGGATCTATAATGAATATGTCACTGTGACTAAAGACATGACAAACATTTTTATGTATGGAGATGGCCCAAAAAGAACCATTGTCACTGGTCGCAGGAGCAACCGTGCTGGCTACACCACCCAAGACACTGCTACTTTTATCGCCATAGGAGCAGGATTTCTTTGCAAATCCATGGGATTCCAAAACACAGCCGGTCCTGAAGGCCACCAAGCCGTAGCCCTTAGAGTCCAATCCGACAAATCTGCCTTCTTTAACTGTAGAATGGATGGCTACCAAGACACTCTCTACGTGCAAACTGAACGTCAATTCTACCGCAATTGTGTCATATCCGGCACCGTAGATTTCATCTTCGGCGACTCAACCACCATCATTCAAAACTCCTTGATCATCGTTCGTCGTCCAATGGACAACCAACAAAACACTGTCACAGCCAATGGTCGTGCCGATCCAAAAGAAGTATCAGGCTTGGTGATCCACAACTGCAGAATTGTGCCAGAACAGAAGCTCTTTGCTGAGAGGTTCAAGATTCCAACATACTTGGGGCGCCCATGGAAGCAATACGCTAGAACTGTGATCATGGAGACTACATTGGGCGACTTCATTAAGCCGGATGGATACATGCCATGGTCTGGAAATTTTGCATTGGAGACTTGTTTGTATTTGGAGTATGGGAACAGAGGACCTGGTGCTAACACCAACAGGAGAGTAAGATGGAAGGGTGTTAAAGTCATTCCAAGAACTGAAGCTTTGAAATTCACTGCTGGATCATTTCTTCAAGGAGGAAGTTGGCTTCCAGGCACTGGTGGGCCTTACCTCTTGGGCTTGAAAGGCTAAAAAAATAAGGGATTGTGCTTGTTGATGAAGAATGTAATATAATTTATTTGATTTCCCATTCTATAACAATTATGCCTTAGATCTTAGTTGGTATTTTGTAAGCAATTGCAAATGGCCTTAATGGTCACAACTTTGAGAAGGCATTCATTTTTTACAAGAATGTTAGCAACAAATATCGTGTTATGTTATTACGAATTTTAACCGTTGCAAAAATT

Coding sequence (CDS)

ATGGTGAATAAGGTGGTCTTGCCTGGAATATCCCTTATTCTTGTTGTTGGTGTAGCACTTGCAGTTGTTGCAGTGGTTAACAAGAACAATAATTCCACGACGGAGAATCTGTCCCCAAAGATGAAGGCAGTCTCAACCATTTGTTCTAATACTAATTACCAAGAAGAATGCCAAAATACACTTACCAATGTTGCTCATAATGCCTCTTCAGATGACCCTAAGGAGTATGTTAAAGCAGCAATTCTTGCCACTATGGAGGAGGTTAAAAAGGGCTATAATTTGACTGATGGCTTTATGATTGAGGCTGCTAACAATGCCAGTATCAAAATGGGTGTTGAGGATTGCAAGGACTTATTGCAATTCGCCATTGCCCAGTTGCAGGCATCTTACACAACTGTTGGCGAACCTGATTTGCACACCAACGCCGATCGTGTCGCTGATATTAAGAATTGGCTCACTGCTGTTATTTCTTATCAACAATCTTGTCTCGATGGACTTGGAGAATTTGATCCAAACCTTCAACAGAAGATGCAAGATGGTCTTGATATTGCTGCTAAGCTTACTAGTAATGCTCTTGCTATTGTTGATACAGTTTCTGATATTCTTCAAAACTTCGGCTTGCAATTCAAGGTTCAACCAAGTGGTCGCAAGCTCCTTGGAACAATAGAGGTTGGGAGCGACGGGTTCCCGACATGGATGTCGGGGGCAGACAGGAAGTTGCTGGCATCAAGAGGAGGCGGTCGAGTGAAACCGAATGCAGTGGTGGCGAAGGATGGTAGCGGACAGTTCAAGACCATTGGTGCTGCATTGGCTGCATACCCAAAGGCCCTAAAAGGAAGATATGTAATCTACGTGAAAGCAGGGATCTATAATGAATATGTCACTGTGACTAAAGACATGACAAACATTTTTATGTATGGAGATGGCCCAAAAAGAACCATTGTCACTGGTCGCAGGAGCAACCGTGCTGGCTACACCACCCAAGACACTGCTACTTTTATCGCCATAGGAGCAGGATTTCTTTGCAAATCCATGGGATTCCAAAACACAGCCGGTCCTGAAGGCCACCAAGCCGTAGCCCTTAGAGTCCAATCCGACAAATCTGCCTTCTTTAACTGTAGAATGGATGGCTACCAAGACACTCTCTACGTGCAAACTGAACGTCAATTCTACCGCAATTGTGTCATATCCGGCACCGTAGATTTCATCTTCGGCGACTCAACCACCATCATTCAAAACTCCTTGATCATCGTTCGTCGTCCAATGGACAACCAACAAAACACTGTCACAGCCAATGGTCGTGCCGATCCAAAAGAAGTATCAGGCTTGGTGATCCACAACTGCAGAATTGTGCCAGAACAGAAGCTCTTTGCTGAGAGGTTCAAGATTCCAACATACTTGGGGCGCCCATGGAAGCAATACGCTAGAACTGTGATCATGGAGACTACATTGGGCGACTTCATTAAGCCGGATGGATACATGCCATGGTCTGGAAATTTTGCATTGGAGACTTGTTTGTATTTGGAGTATGGGAACAGAGGACCTGGTGCTAACACCAACAGGAGAGTAAGATGGAAGGGTGTTAAAGTCATTCCAAGAACTGAAGCTTTGAAATTCACTGCTGGATCATTTCTTCAAGGAGGAAGTTGGCTTCCAGGCACTGGTGGGCCTTACCTCTTGGGCTTGAAAGGCTAA

Protein sequence

MVNKVVLPGISLILVVGVALAVVAVVNKNNNSTTENLSPKMKAVSTICSNTNYQEECQNTLTNVAHNASSDDPKEYVKAAILATMEEVKKGYNLTDGFMIEAANNASIKMGVEDCKDLLQFAIAQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLGEFDPNLQQKMQDGLDIAAKLTSNALAIVDTVSDILQNFGLQFKVQPSGRKLLGTIEVGSDGFPTWMSGADRKLLASRGGGRVKPNAVVAKDGSGQFKTIGAALAAYPKALKGRYVIYVKAGIYNEYVTVTKDMTNIFMYGDGPKRTIVTGRRSNRAGYTTQDTATFIAIGAGFLCKSMGFQNTAGPEGHQAVALRVQSDKSAFFNCRMDGYQDTLYVQTERQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMETTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPRTEALKFTAGSFLQGGSWLPGTGGPYLLGLKG
Homology
BLAST of Bhi12G000902 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 464.2 bits (1193), Expect = 1.5e-130
Identity = 235/561 (41.89%), Postives = 351/561 (62.57%), Query Frame = 0

Query: 4   KVVLPGISLILVVGVALAVV--AVVNKNNNSTTENLSPKMKAVSTICSNTNYQEECQNTL 63
           + V+  IS +L++ + +AV     VNK++N+  E ++  +KA+  +C+ T+Y+E C++TL
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTL 74

Query: 64  TNVAHNASSDDPKEYVKAAILATMEEVKKGYNLTDGFMIEAANNASIKMGVEDCKDLLQF 123
              A + S  DP E VK A  ATM+++      +   MIE   +   KM ++ CK+L+ +
Sbjct: 75  RKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQ-TMIELQKDPRAKMALDQCKELMDY 134

Query: 124 AIAQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLGEFDPNLQQKMQDGL 183
           AI +L  S+  +G+ + H   + +  ++ WL+A IS++Q+CLDG      N  + ++  L
Sbjct: 135 AIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKAL 194

Query: 184 DIAAKLTSNALAIVDTVSDILQNFGLQFKVQPSGRKLLGTIEVGSDGFPTWMSGADRKLL 243
             A +LT N LA+V  +S+ L    +    + + R+LL      S  FP+WM    R+LL
Sbjct: 195 KTAVQLTHNGLAMVTEMSNYLGQMQIP---EMNSRRLL------SQEFPSWMDARARRLL 254

Query: 244 ASRGGGRVKPNAVVAKDGSGQFKTIGAALAAYPKALKGRYVIYVKAGIYNEYVTVTKDMT 303
            +     VKP+ VVA+DGSGQ+KTI  AL   PK     +V+++K GIY EYV V + MT
Sbjct: 255 -NAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSMT 314

Query: 304 NIFMYGDGPKRTIVTGRRSNRAGYTTQDTATFIAIGAGFLCKSMGFQNTAGPEGHQAVAL 363
           ++   GDGP +T+++G +S + G TT  TAT   +G  F+ K++ F+NTAG   HQAVA+
Sbjct: 315 HLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVAI 374

Query: 364 RVQSDKSAFFNCRMDGYQDTLYVQTERQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRRP 423
           RV +D+S F+NC+ DGYQDTLY  + RQFYR+C ISGT+DF+FGD+  + QN  ++VR+P
Sbjct: 375 RVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRKP 434

Query: 424 MDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIME 483
           + NQ   +TA+GR DP+E +G V+  C IV E    A + +  TYLGRPWK+Y+RT+IM 
Sbjct: 435 LLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIMN 494

Query: 484 TTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPRTEALKFTA 543
           T + DF+ P+G+ PW G F L T  Y E  N GPGA   +RV W G+K +   E LKFT 
Sbjct: 495 TFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFTP 554

Query: 544 GSFLQGGSWLPGTGGPYLLGL 563
             ++QG +W+PG G PY+LGL
Sbjct: 555 AQYIQGDAWIPGKGVPYILGL 562

BLAST of Bhi12G000902 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 449.1 bits (1154), Expect = 5.1e-126
Identity = 226/559 (40.43%), Postives = 347/559 (62.08%), Query Frame = 0

Query: 10  ISLILVVGVALAVVAVVNKN----NNSTTENLSPKMKAVSTICSNTNYQEECQNTLTNVA 69
           IS +L++ + +AV   V+ N    ++     ++  +KAV  +C+ T+Y++ C++TL  + 
Sbjct: 20  ISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTL--IK 79

Query: 70  HNASSDDPKEYVKAAILATMEEVKKGYNLTDGFMIEAANNASIKMGVEDCKDLLQFAIAQ 129
           +  ++ DP E VK A   TM+++      +   M E   ++  +M ++ CK+L+ +A+ +
Sbjct: 80  NGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIM-ELQKDSRTRMALDQCKELMDYALDE 139

Query: 130 LQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLGEFDPNLQQKMQDGLDIAA 189
           L  S+  +G+ + H   + + +++ WL+A IS++++CL+G      N  + M+  L  A 
Sbjct: 140 LSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALKTAI 199

Query: 190 KLTSNALAIVDTVSDILQNFGLQFKVQP-SGRKLLGTIEVGSDGFPTWMSGADRKLL-AS 249
           +LT N LAI+  +S    NF  Q ++   + R+LL      ++GFP+W+    RKLL A+
Sbjct: 200 ELTHNGLAIISEMS----NFVGQMQIPGLNSRRLL------AEGFPSWVDQRGRKLLQAA 259

Query: 250 RGGGRVKPNAVVAKDGSGQFKTIGAALAAYPKALKGRYVIYVKAGIYNEYVTVTKDMTNI 309
                VKP+ VVA+DGSGQ+KTI  AL   PK     +V+++KAG+Y EYV V K M+++
Sbjct: 260 AAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMSHL 319

Query: 310 FMYGDGPKRTIVTGRRSNRAGYTTQDTATFIAIGAGFLCKSMGFQNTAGPEGHQAVALRV 369
              GDGP +TI++G ++ + G TT  TAT   +G  F+ K++GF+NTAG   HQAVA+RV
Sbjct: 320 VFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAVRV 379

Query: 370 QSDKSAFFNCRMDGYQDTLYVQTERQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRRPMD 429
           QSD+S FFNCR DGYQDTLY  + RQF+R+C ISGT+DF+FGD+  + QN  ++VR+P+ 
Sbjct: 380 QSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLP 439

Query: 430 NQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMETT 489
           NQ   +TA+GR DP+E +G V   C I  E    A +     YLGRPWK+Y+RT+IM T 
Sbjct: 440 NQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMNTF 499

Query: 490 LGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPRTEALKFTAGS 549
           + DF++P G+ PW G+F L+T  Y E  N GPG+    RV W G+K +   + LKFT   
Sbjct: 500 IPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTPAQ 559

Query: 550 FLQGGSWLPGTGGPYLLGL 563
           ++QG  W+PG G PY  GL
Sbjct: 560 YIQGDDWIPGKGVPYTTGL 565

BLAST of Bhi12G000902 vs. TAIR 10
Match: AT2G47030.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 447.2 bits (1149), Expect = 2.0e-125
Identity = 264/601 (43.93%), Postives = 359/601 (59.73%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVVNKNNNSTTENLSPKMKAVSTICSNTNYQEECQNT 60
           M+ KVV+   S++L+VGVA+ VVA +NKN ++   NLSP+MKAV  IC +T+ +  C  T
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNGDA---NLSPQMKAVQGICQSTSDKASCVKT 60

Query: 61  LTNVAHNASSDDPKEYVKAAILATMEEVKKGYNLTDGFMIEAANNAS--IKMGVEDCKDL 120
           L  V     S+DP + +KA +LAT +E+ K  N T    +   ++ S   K  ++ CK +
Sbjct: 61  LEPV----KSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCKRV 120

Query: 121 LQFAIAQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLGEFDPNLQQKMQ 180
             +A+  L      +GE DL     ++  +K WL  V +YQ  CLD + E D  L++ + 
Sbjct: 121 FMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKAIG 180

Query: 181 DGLDIAAKLTSNALAIVDTV----------SDILQNF--GLQFKVQP------------- 240
           +G+  +  LT+NA+ I  TV           D L+N   G+     P             
Sbjct: 181 EGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADPDG 240

Query: 241 SGRKLLGTIEVGSDGFPTWMSGADRKLLA--------SRGGG-RVKPNAVVAKDGSGQFK 300
             R+LL  I+    G PTW+SGADRKL+A         RGGG RV+ N VVAKDGSGQFK
Sbjct: 241 PARRLLEDID--ETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQFK 300

Query: 301 TIGAALAAYPKALKGRYVIYVKAGIYNEYVTVTKDMTNIFMYGDGPKRTIVTGRRS--NR 360
           T+  A+ A P+  +GR +IY+KAG+Y E V + K   NIFM+GDG ++T+++  RS    
Sbjct: 301 TVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVALS 360

Query: 361 AGYTTQDTATFIAIGAGFLCKSMGFQNTAGPEGHQAVALRVQSDKSAFFNCRMDGYQDTL 420
            G TT  +AT      GF+ K MGF+NTAGP GHQA A+RV  D++  FNCR DGYQDTL
Sbjct: 361 RGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQDTL 420

Query: 421 YVQTERQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRRPMDNQQNTVTANGRADPKEVS- 480
           YV   RQFYRNCV+SGTVDFIFG S T+IQN+LI+VR+    Q NTVTA+G      +  
Sbjct: 421 YVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGMKI 480

Query: 481 GLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMETTLGDFIKPDGYMPWSGNFA 540
           G+V+ NCRIVP++KL  ER  + TYLGRPWK+++ TVIM T +GD I+P+G+  W G   
Sbjct: 481 GIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGESF 540

Query: 541 LETCLYLEYGNRGPGANTNRRVRWKGVKVIPRTEALKFTAGSFLQGGSWLPGTGGPYLLG 563
            ++C Y+EY NRGPGA  NRRV W  V      E   FTA ++L   +W+     P  +G
Sbjct: 541 HKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNGFTAANWLGPINWIQEANVPVTIG 588

BLAST of Bhi12G000902 vs. TAIR 10
Match: AT2G26450.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 443.7 bits (1140), Expect = 2.2e-124
Identity = 234/523 (44.74%), Postives = 332/523 (63.48%), Query Frame = 0

Query: 42  KAVSTICSNTNYQEECQNTLTNVAHNA-SSDDPKEYVKAAILATMEEVKKGYNLTDGFMI 101
           K + T+CS+T Y + C+ TL N      + D+P  ++K+AI A  E++     + +  + 
Sbjct: 107 KIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDL---VLEKVLS 166

Query: 102 EAANNASIKMGVEDCKDLLQFAIAQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQ 161
               N   K  +E CK L++ A  +  AS   +   ++++    V D+++WL+AV+SYQ+
Sbjct: 167 LKTENQDDKDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQE 226

Query: 162 SCLDGLGEFDPNLQQKMQDGLDIAAKLTSNALAIVDTVSDILQNFGLQFKVQPSGRKLLG 221
           +CLDG  E   NL+ +++  ++ +  LTSN+LA++ T +   +N     KV    R LL 
Sbjct: 227 TCLDGFEE--GNLKSEVKTSVNSSQVLTSNSLALIKTFT---ENLSPVMKVVE--RHLL- 286

Query: 222 TIEVGSDGFPTWMSGADRKLLASRGGGRVKPNAVVAKDGSGQFKTIGAALAAYPKALKGR 281
                 D  P+W+S  DR++L +     +KPNA VAKDGSG F TI  AL A P+  +GR
Sbjct: 287 ------DDIPSWVSNDDRRMLRAVDVKALKPNATVAKDGSGDFTTINDALRAMPEKYEGR 346

Query: 282 YVIYVKAGIYNEYVTVTKDMTNIFMYGDGPKRTIVTGRRSNRAGYTTQDTATFIAIGAGF 341
           Y+IYVK GIY+EYVTV K   N+ M GDG ++TIVTG +S+     T  TATF+A G GF
Sbjct: 347 YIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEGF 406

Query: 342 LCKSMGFQNTAGPEGHQAVALRVQSDKSAFFNCRMDGYQDTLYVQTERQFYRNCVISGTV 401
           + +SMGF+NTAGPEGHQAVA+RVQSD+S F NCR +GYQDTLY  T RQ+YR+CVI GT+
Sbjct: 407 MAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGTI 466

Query: 402 DFIFGDSTTIIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAER 461
           DFIFGD+  I QN  I +R+ +  Q+NTVTA GR D  + +G V+HNC+I   + L   +
Sbjct: 467 DFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPVK 526

Query: 462 FKIPTYLGRPWKQYARTVIMETTLGDFIKPDGYMPW-SGNFALETCLYLEYGNRGPGANT 521
            +  +YLGRPWK Y+RT+IME+ + + I P G++ W   +FA++T  Y EY N+G   +T
Sbjct: 527 EEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGDT 586

Query: 522 NRRVRWKGVKVIPRTEALKFTAGSFLQGGSWLPGTGGPYLLGL 563
             RV+W G KVI + EAL +T G FLQ G W+  +G P  LGL
Sbjct: 587 TSRVKWPGFKVINKEEALNYTVGPFLQ-GDWISASGSPVKLGL 611

BLAST of Bhi12G000902 vs. TAIR 10
Match: AT2G47040.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 436.4 bits (1121), Expect = 3.4e-122
Identity = 261/613 (42.58%), Postives = 358/613 (58.40%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVVNKNNNSTTENLSPKMKAVSTICSNTNYQEECQNT 60
           M+ KVV+   S++L+VGVA+ VVA +NKN ++   NLSP+MKAV  IC  T+ +  C  T
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAYINKNGDA---NLSPQMKAVRGICEATSDKASCVKT 60

Query: 61  LTNVAHNASSDDPKEYVKAAILATMEEVKKGYNLTD------GFMIEAANNASIKMGVED 120
           L  V     SDDP + +KA +LAT + + +  N T       G  I   N A +    + 
Sbjct: 61  LEPV----KSDDPNKLIKAFMLATRDAITQSSNFTGKTEGNLGSGISPNNKAVL----DY 120

Query: 121 CKDLLQFAIAQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLGEFDPNLQ 180
           CK +  +A+  L      +GE DL+    ++  +K WLT V +YQ  CLD + E D  L+
Sbjct: 121 CKKVFMYALEDLSTIVEEMGE-DLNQIGSKIDQLKQWLTGVYNYQTDCLDDIEEDD--LR 180

Query: 181 QKMQDGLDIAAKLTSNALAIVDTVSDILQNFGLQ---FK--------------------- 240
           + + +G+  +  LTSNA+ I  TV   +    L+   FK                     
Sbjct: 181 KTIGEGIASSKILTSNAIDIFHTVVSAMAKLNLKVEDFKNMTGGIFAPSDKGAAPVNKGT 240

Query: 241 ---------VQPSG--RKLLGTIEVGSDGFPTWMSGADRKLL------ASRGGGRVKPNA 300
                      P G  R+LL  I+    G PTW+SGADRKL+      ++ GG R++   
Sbjct: 241 PPVADDSPVADPDGPARRLLEDID--ETGIPTWVSGADRKLMTKAGRGSNDGGARIRATF 300

Query: 301 VVAKDGSGQFKTIGAALAAYPKALKGRYVIYVKAGIYNEYVTVTKDMTNIFMYGDGPKRT 360
           VVAKDGSGQFKT+  A+ A P+   GR +I++KAGIY E V + K   NIFM+GDG ++T
Sbjct: 301 VVAKDGSGQFKTVQQAVNACPEKNPGRCIIHIKAGIYREQVIIPKKKNNIFMFGDGARKT 360

Query: 361 IVTGRRSNR--AGYTTQDTATFIAIGAGFLCKSMGFQNTAGPEGHQAVALRVQSDKSAFF 420
           +++  RS +   G TT  + T      GF+ K +GF+NTAGP GHQAVA+RV  D++  F
Sbjct: 361 VISYNRSVKLSPGTTTSLSGTVQVESEGFMAKWIGFKNTAGPMGHQAVAIRVNGDRAVIF 420

Query: 421 NCRMDGYQDTLYVQTERQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRRPMDNQQNTVTA 480
           NCR DGYQDTLYV   RQFYRN V+SGTVDFIFG S T+IQNSLI+VR+    Q NTVTA
Sbjct: 421 NCRFDGYQDTLYVNNGRQFYRNIVVSGTVDFIFGKSATVIQNSLIVVRKGNKGQFNTVTA 480

Query: 481 NGRADPKEVS-GLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMETTLGDFIKP 540
           +G      +  G+V+ NCRIVP++KL AER  + +YLGRPWK+++ TVI+ + +GD I+P
Sbjct: 481 DGNEKGLAMKIGIVLQNCRIVPDKKLAAERLIVESYLGRPWKKFSTTVIINSEIGDVIRP 540

Query: 541 DGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVI-PRTEALKFTAGSFLQGGS 563
           +G+  W G    ++C Y+EY NRGPGA TNRRV W  VK+     E   FT  ++L   +
Sbjct: 541 EGWKIWDGESFHKSCRYVEYNNRGPGAITNRRVNW--VKIARSAAEVNDFTVANWLGPIN 595

BLAST of Bhi12G000902 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 2.2e-129
Identity = 235/561 (41.89%), Postives = 351/561 (62.57%), Query Frame = 0

Query: 4   KVVLPGISLILVVGVALAVV--AVVNKNNNSTTENLSPKMKAVSTICSNTNYQEECQNTL 63
           + V+  IS +L++ + +AV     VNK++N+  E ++  +KA+  +C+ T+Y+E C++TL
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTL 74

Query: 64  TNVAHNASSDDPKEYVKAAILATMEEVKKGYNLTDGFMIEAANNASIKMGVEDCKDLLQF 123
              A + S  DP E VK A  ATM+++      +   MIE   +   KM ++ CK+L+ +
Sbjct: 75  RKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQ-TMIELQKDPRAKMALDQCKELMDY 134

Query: 124 AIAQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLGEFDPNLQQKMQDGL 183
           AI +L  S+  +G+ + H   + +  ++ WL+A IS++Q+CLDG      N  + ++  L
Sbjct: 135 AIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKAL 194

Query: 184 DIAAKLTSNALAIVDTVSDILQNFGLQFKVQPSGRKLLGTIEVGSDGFPTWMSGADRKLL 243
             A +LT N LA+V  +S+ L    +    + + R+LL      S  FP+WM    R+LL
Sbjct: 195 KTAVQLTHNGLAMVTEMSNYLGQMQIP---EMNSRRLL------SQEFPSWMDARARRLL 254

Query: 244 ASRGGGRVKPNAVVAKDGSGQFKTIGAALAAYPKALKGRYVIYVKAGIYNEYVTVTKDMT 303
            +     VKP+ VVA+DGSGQ+KTI  AL   PK     +V+++K GIY EYV V + MT
Sbjct: 255 -NAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSMT 314

Query: 304 NIFMYGDGPKRTIVTGRRSNRAGYTTQDTATFIAIGAGFLCKSMGFQNTAGPEGHQAVAL 363
           ++   GDGP +T+++G +S + G TT  TAT   +G  F+ K++ F+NTAG   HQAVA+
Sbjct: 315 HLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVAI 374

Query: 364 RVQSDKSAFFNCRMDGYQDTLYVQTERQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRRP 423
           RV +D+S F+NC+ DGYQDTLY  + RQFYR+C ISGT+DF+FGD+  + QN  ++VR+P
Sbjct: 375 RVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRKP 434

Query: 424 MDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIME 483
           + NQ   +TA+GR DP+E +G V+  C IV E    A + +  TYLGRPWK+Y+RT+IM 
Sbjct: 435 LLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIMN 494

Query: 484 TTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPRTEALKFTA 543
           T + DF+ P+G+ PW G F L T  Y E  N GPGA   +RV W G+K +   E LKFT 
Sbjct: 495 TFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFTP 554

Query: 544 GSFLQGGSWLPGTGGPYLLGL 563
             ++QG +W+PG G PY+LGL
Sbjct: 555 AQYIQGDAWIPGKGVPYILGL 562

BLAST of Bhi12G000902 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 449.1 bits (1154), Expect = 7.2e-125
Identity = 226/559 (40.43%), Postives = 347/559 (62.08%), Query Frame = 0

Query: 10  ISLILVVGVALAVVAVVNKN----NNSTTENLSPKMKAVSTICSNTNYQEECQNTLTNVA 69
           IS +L++ + +AV   V+ N    ++     ++  +KAV  +C+ T+Y++ C++TL  + 
Sbjct: 20  ISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTL--IK 79

Query: 70  HNASSDDPKEYVKAAILATMEEVKKGYNLTDGFMIEAANNASIKMGVEDCKDLLQFAIAQ 129
           +  ++ DP E VK A   TM+++      +   M E   ++  +M ++ CK+L+ +A+ +
Sbjct: 80  NGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIM-ELQKDSRTRMALDQCKELMDYALDE 139

Query: 130 LQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLGEFDPNLQQKMQDGLDIAA 189
           L  S+  +G+ + H   + + +++ WL+A IS++++CL+G      N  + M+  L  A 
Sbjct: 140 LSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALKTAI 199

Query: 190 KLTSNALAIVDTVSDILQNFGLQFKVQP-SGRKLLGTIEVGSDGFPTWMSGADRKLL-AS 249
           +LT N LAI+  +S    NF  Q ++   + R+LL      ++GFP+W+    RKLL A+
Sbjct: 200 ELTHNGLAIISEMS----NFVGQMQIPGLNSRRLL------AEGFPSWVDQRGRKLLQAA 259

Query: 250 RGGGRVKPNAVVAKDGSGQFKTIGAALAAYPKALKGRYVIYVKAGIYNEYVTVTKDMTNI 309
                VKP+ VVA+DGSGQ+KTI  AL   PK     +V+++KAG+Y EYV V K M+++
Sbjct: 260 AAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMSHL 319

Query: 310 FMYGDGPKRTIVTGRRSNRAGYTTQDTATFIAIGAGFLCKSMGFQNTAGPEGHQAVALRV 369
              GDGP +TI++G ++ + G TT  TAT   +G  F+ K++GF+NTAG   HQAVA+RV
Sbjct: 320 VFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAVRV 379

Query: 370 QSDKSAFFNCRMDGYQDTLYVQTERQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRRPMD 429
           QSD+S FFNCR DGYQDTLY  + RQF+R+C ISGT+DF+FGD+  + QN  ++VR+P+ 
Sbjct: 380 QSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLP 439

Query: 430 NQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMETT 489
           NQ   +TA+GR DP+E +G V   C I  E    A +     YLGRPWK+Y+RT+IM T 
Sbjct: 440 NQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMNTF 499

Query: 490 LGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPRTEALKFTAGS 549
           + DF++P G+ PW G+F L+T  Y E  N GPG+    RV W G+K +   + LKFT   
Sbjct: 500 IPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTPAQ 559

Query: 550 FLQGGSWLPGTGGPYLLGL 563
           ++QG  W+PG G PY  GL
Sbjct: 560 YIQGDDWIPGKGVPYTTGL 565

BLAST of Bhi12G000902 vs. ExPASy Swiss-Prot
Match: O80722 (Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 2.7e-124
Identity = 264/601 (43.93%), Postives = 359/601 (59.73%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVVNKNNNSTTENLSPKMKAVSTICSNTNYQEECQNT 60
           M+ KVV+   S++L+VGVA+ VVA +NKN ++   NLSP+MKAV  IC +T+ +  C  T
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNGDA---NLSPQMKAVQGICQSTSDKASCVKT 60

Query: 61  LTNVAHNASSDDPKEYVKAAILATMEEVKKGYNLTDGFMIEAANNAS--IKMGVEDCKDL 120
           L  V     S+DP + +KA +LAT +E+ K  N T    +   ++ S   K  ++ CK +
Sbjct: 61  LEPV----KSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCKRV 120

Query: 121 LQFAIAQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLGEFDPNLQQKMQ 180
             +A+  L      +GE DL     ++  +K WL  V +YQ  CLD + E D  L++ + 
Sbjct: 121 FMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKAIG 180

Query: 181 DGLDIAAKLTSNALAIVDTV----------SDILQNF--GLQFKVQP------------- 240
           +G+  +  LT+NA+ I  TV           D L+N   G+     P             
Sbjct: 181 EGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADPDG 240

Query: 241 SGRKLLGTIEVGSDGFPTWMSGADRKLLA--------SRGGG-RVKPNAVVAKDGSGQFK 300
             R+LL  I+    G PTW+SGADRKL+A         RGGG RV+ N VVAKDGSGQFK
Sbjct: 241 PARRLLEDID--ETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQFK 300

Query: 301 TIGAALAAYPKALKGRYVIYVKAGIYNEYVTVTKDMTNIFMYGDGPKRTIVTGRRS--NR 360
           T+  A+ A P+  +GR +IY+KAG+Y E V + K   NIFM+GDG ++T+++  RS    
Sbjct: 301 TVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVALS 360

Query: 361 AGYTTQDTATFIAIGAGFLCKSMGFQNTAGPEGHQAVALRVQSDKSAFFNCRMDGYQDTL 420
            G TT  +AT      GF+ K MGF+NTAGP GHQA A+RV  D++  FNCR DGYQDTL
Sbjct: 361 RGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQDTL 420

Query: 421 YVQTERQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRRPMDNQQNTVTANGRADPKEVS- 480
           YV   RQFYRNCV+SGTVDFIFG S T+IQN+LI+VR+    Q NTVTA+G      +  
Sbjct: 421 YVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGMKI 480

Query: 481 GLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMETTLGDFIKPDGYMPWSGNFA 540
           G+V+ NCRIVP++KL  ER  + TYLGRPWK+++ TVIM T +GD I+P+G+  W G   
Sbjct: 481 GIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGESF 540

Query: 541 LETCLYLEYGNRGPGANTNRRVRWKGVKVIPRTEALKFTAGSFLQGGSWLPGTGGPYLLG 563
            ++C Y+EY NRGPGA  NRRV W  V      E   FTA ++L   +W+     P  +G
Sbjct: 541 HKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNGFTAANWLGPINWIQEANVPVTIG 588

BLAST of Bhi12G000902 vs. ExPASy Swiss-Prot
Match: Q7Y201 (Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3702 GN=PME13 PE=2 SV=2)

HSP 1 Score: 443.7 bits (1140), Expect = 3.0e-123
Identity = 234/523 (44.74%), Postives = 332/523 (63.48%), Query Frame = 0

Query: 42  KAVSTICSNTNYQEECQNTLTNVAHNA-SSDDPKEYVKAAILATMEEVKKGYNLTDGFMI 101
           K + T+CS+T Y + C+ TL N      + D+P  ++K+AI A  E++     + +  + 
Sbjct: 107 KIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDL---VLEKVLS 166

Query: 102 EAANNASIKMGVEDCKDLLQFAIAQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQ 161
               N   K  +E CK L++ A  +  AS   +   ++++    V D+++WL+AV+SYQ+
Sbjct: 167 LKTENQDDKDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQE 226

Query: 162 SCLDGLGEFDPNLQQKMQDGLDIAAKLTSNALAIVDTVSDILQNFGLQFKVQPSGRKLLG 221
           +CLDG  E   NL+ +++  ++ +  LTSN+LA++ T +   +N     KV    R LL 
Sbjct: 227 TCLDGFEE--GNLKSEVKTSVNSSQVLTSNSLALIKTFT---ENLSPVMKVVE--RHLL- 286

Query: 222 TIEVGSDGFPTWMSGADRKLLASRGGGRVKPNAVVAKDGSGQFKTIGAALAAYPKALKGR 281
                 D  P+W+S  DR++L +     +KPNA VAKDGSG F TI  AL A P+  +GR
Sbjct: 287 ------DDIPSWVSNDDRRMLRAVDVKALKPNATVAKDGSGDFTTINDALRAMPEKYEGR 346

Query: 282 YVIYVKAGIYNEYVTVTKDMTNIFMYGDGPKRTIVTGRRSNRAGYTTQDTATFIAIGAGF 341
           Y+IYVK GIY+EYVTV K   N+ M GDG ++TIVTG +S+     T  TATF+A G GF
Sbjct: 347 YIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEGF 406

Query: 342 LCKSMGFQNTAGPEGHQAVALRVQSDKSAFFNCRMDGYQDTLYVQTERQFYRNCVISGTV 401
           + +SMGF+NTAGPEGHQAVA+RVQSD+S F NCR +GYQDTLY  T RQ+YR+CVI GT+
Sbjct: 407 MAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGTI 466

Query: 402 DFIFGDSTTIIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAER 461
           DFIFGD+  I QN  I +R+ +  Q+NTVTA GR D  + +G V+HNC+I   + L   +
Sbjct: 467 DFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPVK 526

Query: 462 FKIPTYLGRPWKQYARTVIMETTLGDFIKPDGYMPW-SGNFALETCLYLEYGNRGPGANT 521
            +  +YLGRPWK Y+RT+IME+ + + I P G++ W   +FA++T  Y EY N+G   +T
Sbjct: 527 EEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGDT 586

Query: 522 NRRVRWKGVKVIPRTEALKFTAGSFLQGGSWLPGTGGPYLLGL 563
             RV+W G KVI + EAL +T G FLQ G W+  +G P  LGL
Sbjct: 587 TSRVKWPGFKVINKEEALNYTVGPFLQ-GDWISASGSPVKLGL 611

BLAST of Bhi12G000902 vs. ExPASy Swiss-Prot
Match: Q5MFV8 (Pectinesterase 5 OS=Arabidopsis thaliana OX=3702 GN=PME5 PE=1 SV=2)

HSP 1 Score: 436.4 bits (1121), Expect = 4.9e-121
Identity = 261/613 (42.58%), Postives = 358/613 (58.40%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVVNKNNNSTTENLSPKMKAVSTICSNTNYQEECQNT 60
           M+ KVV+   S++L+VGVA+ VVA +NKN ++   NLSP+MKAV  IC  T+ +  C  T
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAYINKNGDA---NLSPQMKAVRGICEATSDKASCVKT 60

Query: 61  LTNVAHNASSDDPKEYVKAAILATMEEVKKGYNLTD------GFMIEAANNASIKMGVED 120
           L  V     SDDP + +KA +LAT + + +  N T       G  I   N A +    + 
Sbjct: 61  LEPV----KSDDPNKLIKAFMLATRDAITQSSNFTGKTEGNLGSGISPNNKAVL----DY 120

Query: 121 CKDLLQFAIAQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLGEFDPNLQ 180
           CK +  +A+  L      +GE DL+    ++  +K WLT V +YQ  CLD + E D  L+
Sbjct: 121 CKKVFMYALEDLSTIVEEMGE-DLNQIGSKIDQLKQWLTGVYNYQTDCLDDIEEDD--LR 180

Query: 181 QKMQDGLDIAAKLTSNALAIVDTVSDILQNFGLQ---FK--------------------- 240
           + + +G+  +  LTSNA+ I  TV   +    L+   FK                     
Sbjct: 181 KTIGEGIASSKILTSNAIDIFHTVVSAMAKLNLKVEDFKNMTGGIFAPSDKGAAPVNKGT 240

Query: 241 ---------VQPSG--RKLLGTIEVGSDGFPTWMSGADRKLL------ASRGGGRVKPNA 300
                      P G  R+LL  I+    G PTW+SGADRKL+      ++ GG R++   
Sbjct: 241 PPVADDSPVADPDGPARRLLEDID--ETGIPTWVSGADRKLMTKAGRGSNDGGARIRATF 300

Query: 301 VVAKDGSGQFKTIGAALAAYPKALKGRYVIYVKAGIYNEYVTVTKDMTNIFMYGDGPKRT 360
           VVAKDGSGQFKT+  A+ A P+   GR +I++KAGIY E V + K   NIFM+GDG ++T
Sbjct: 301 VVAKDGSGQFKTVQQAVNACPEKNPGRCIIHIKAGIYREQVIIPKKKNNIFMFGDGARKT 360

Query: 361 IVTGRRSNR--AGYTTQDTATFIAIGAGFLCKSMGFQNTAGPEGHQAVALRVQSDKSAFF 420
           +++  RS +   G TT  + T      GF+ K +GF+NTAGP GHQAVA+RV  D++  F
Sbjct: 361 VISYNRSVKLSPGTTTSLSGTVQVESEGFMAKWIGFKNTAGPMGHQAVAIRVNGDRAVIF 420

Query: 421 NCRMDGYQDTLYVQTERQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRRPMDNQQNTVTA 480
           NCR DGYQDTLYV   RQFYRN V+SGTVDFIFG S T+IQNSLI+VR+    Q NTVTA
Sbjct: 421 NCRFDGYQDTLYVNNGRQFYRNIVVSGTVDFIFGKSATVIQNSLIVVRKGNKGQFNTVTA 480

Query: 481 NGRADPKEVS-GLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMETTLGDFIKP 540
           +G      +  G+V+ NCRIVP++KL AER  + +YLGRPWK+++ TVI+ + +GD I+P
Sbjct: 481 DGNEKGLAMKIGIVLQNCRIVPDKKLAAERLIVESYLGRPWKKFSTTVIINSEIGDVIRP 540

Query: 541 DGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVI-PRTEALKFTAGSFLQGGS 563
           +G+  W G    ++C Y+EY NRGPGA TNRRV W  VK+     E   FT  ++L   +
Sbjct: 541 EGWKIWDGESFHKSCRYVEYNNRGPGAITNRRVNW--VKIARSAAEVNDFTVANWLGPIN 595

BLAST of Bhi12G000902 vs. NCBI nr
Match: XP_038875878.1 (pectinesterase-like [Benincasa hispida])

HSP 1 Score: 1130.9 bits (2924), Expect = 0.0e+00
Identity = 564/564 (100.00%), Postives = 564/564 (100.00%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVVNKNNNSTTENLSPKMKAVSTICSNTNYQEECQNT 60
           MVNKVVLPGISLILVVGVALAVVAVVNKNNNSTTENLSPKMKAVSTICSNTNYQEECQNT
Sbjct: 1   MVNKVVLPGISLILVVGVALAVVAVVNKNNNSTTENLSPKMKAVSTICSNTNYQEECQNT 60

Query: 61  LTNVAHNASSDDPKEYVKAAILATMEEVKKGYNLTDGFMIEAANNASIKMGVEDCKDLLQ 120
           LTNVAHNASSDDPKEYVKAAILATMEEVKKGYNLTDGFMIEAANNASIKMGVEDCKDLLQ
Sbjct: 61  LTNVAHNASSDDPKEYVKAAILATMEEVKKGYNLTDGFMIEAANNASIKMGVEDCKDLLQ 120

Query: 121 FAIAQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLGEFDPNLQQKMQDG 180
           FAIAQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLGEFDPNLQQKMQDG
Sbjct: 121 FAIAQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLGEFDPNLQQKMQDG 180

Query: 181 LDIAAKLTSNALAIVDTVSDILQNFGLQFKVQPSGRKLLGTIEVGSDGFPTWMSGADRKL 240
           LDIAAKLTSNALAIVDTVSDILQNFGLQFKVQPSGRKLLGTIEVGSDGFPTWMSGADRKL
Sbjct: 181 LDIAAKLTSNALAIVDTVSDILQNFGLQFKVQPSGRKLLGTIEVGSDGFPTWMSGADRKL 240

Query: 241 LASRGGGRVKPNAVVAKDGSGQFKTIGAALAAYPKALKGRYVIYVKAGIYNEYVTVTKDM 300
           LASRGGGRVKPNAVVAKDGSGQFKTIGAALAAYPKALKGRYVIYVKAGIYNEYVTVTKDM
Sbjct: 241 LASRGGGRVKPNAVVAKDGSGQFKTIGAALAAYPKALKGRYVIYVKAGIYNEYVTVTKDM 300

Query: 301 TNIFMYGDGPKRTIVTGRRSNRAGYTTQDTATFIAIGAGFLCKSMGFQNTAGPEGHQAVA 360
           TNIFMYGDGPKRTIVTGRRSNRAGYTTQDTATFIAIGAGFLCKSMGFQNTAGPEGHQAVA
Sbjct: 301 TNIFMYGDGPKRTIVTGRRSNRAGYTTQDTATFIAIGAGFLCKSMGFQNTAGPEGHQAVA 360

Query: 361 LRVQSDKSAFFNCRMDGYQDTLYVQTERQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRR 420
           LRVQSDKSAFFNCRMDGYQDTLYVQTERQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRR
Sbjct: 361 LRVQSDKSAFFNCRMDGYQDTLYVQTERQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRR 420

Query: 421 PMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIM 480
           PMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIM
Sbjct: 421 PMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIM 480

Query: 481 ETTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPRTEALKFT 540
           ETTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPRTEALKFT
Sbjct: 481 ETTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPRTEALKFT 540

Query: 541 AGSFLQGGSWLPGTGGPYLLGLKG 565
           AGSFLQGGSWLPGTGGPYLLGLKG
Sbjct: 541 AGSFLQGGSWLPGTGGPYLLGLKG 564

BLAST of Bhi12G000902 vs. NCBI nr
Match: KAA0050275.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYJ98132.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1007.3 bits (2603), Expect = 5.3e-290
Identity = 498/566 (87.99%), Postives = 534/566 (94.35%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVV--NKNNNSTTENLSPKMKAVSTICSNTNYQEECQ 60
           MVNKV+LPGISLILVVGVALAVVA+V  NK++++ TE+LSPKMKAVSTIC+N NYQ+ECQ
Sbjct: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLTNVAHNASSDDPKEYVKAAILATMEEVKKGYNLTDGFMIEAANNASIKMGVEDCKDL 120
           NTL+N AHNASSDDPKEYV+AAIL+T+EEVKKGYNLTDGFMIEAANN SIKMGVEDC+DL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIAQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLGEFDPNLQQKMQ 180
           LQFAI QLQASY+TVGEPDLHTNADRVADIKNWLT+VISYQQSCLDGL EFDP L+QKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDIAAKLTSNALAIVDTVSDILQNFGLQFKVQPSGRKLLGTIEVGSDGFPTWMSGADR 240
           DGL  A KLTSNALAIVD VSDIL +FGLQ K QPSGR+LLGT EVG DG+PTW++GADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLASRGGG-RVKPNAVVAKDGSGQFKTIGAALAAYPKALKGRYVIYVKAGIYNEYVTVT 300
           KLLASRGGG +VKPNAVVAKDGSGQFKTI AALAAYPKAL+GRYVIYVKAGIYNEYV +T
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMTNIFMYGDGPKRTIVTGRRSNRAGYTTQDTATFIAIGAGFLCKSMGFQNTAGPEGHQ 360
           KDM NIFMYGDGPK+TIVTG +SNR G+TTQDTATFIAIG GFLCKSMGFQNTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDKSAFFNCRMDGYQDTLYVQTERQFYRNCVISGTVDFIFGDSTTIIQNSLII 420
           AVALRVQSD+SAFFNCRMDGYQDTLYVQT+RQFYRNCVISGTVDFIFGDSTTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMETTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPRTEAL 540
           VIME+TLGDFI+P GYMPWSGNFALETCLYLEYGNRGPGA TNRRVRWKGVKVI R EA+
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 KFTAGSFLQGGSWLPGTGGPYLLGLK 564
           +FTAG FLQG +WLP TGGPYLLGLK
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLK 566

BLAST of Bhi12G000902 vs. NCBI nr
Match: XP_008466374.1 (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 1006.1 bits (2600), Expect = 1.2e-289
Identity = 497/566 (87.81%), Postives = 534/566 (94.35%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVV--NKNNNSTTENLSPKMKAVSTICSNTNYQEECQ 60
           MVNKV+LPGI+LILVVGVALAVVA+V  NK++++ TE+LSPKMKAVSTIC+N NYQ+ECQ
Sbjct: 1   MVNKVILPGITLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLTNVAHNASSDDPKEYVKAAILATMEEVKKGYNLTDGFMIEAANNASIKMGVEDCKDL 120
           NTL+N AHNASSDDPKEYV+AAIL+T+EEVKKGYNLTDGFMIEAANN SIKMGVEDC+DL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIAQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLGEFDPNLQQKMQ 180
           LQFAI QLQASY+TVGEPDLHTNADRVADIKNWLT+VISYQQSCLDGL EFDP L+QKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDIAAKLTSNALAIVDTVSDILQNFGLQFKVQPSGRKLLGTIEVGSDGFPTWMSGADR 240
           DGL  A KLTSNALAIVD VSDIL +FGLQ K QPSGR+LLGT EVG DG+PTW++GADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLASRGGG-RVKPNAVVAKDGSGQFKTIGAALAAYPKALKGRYVIYVKAGIYNEYVTVT 300
           KLLASRGGG +VKPNAVVAKDGSGQFKTI AALAAYPKAL+GRYVIYVKAGIYNEYV +T
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMTNIFMYGDGPKRTIVTGRRSNRAGYTTQDTATFIAIGAGFLCKSMGFQNTAGPEGHQ 360
           KDM NIFMYGDGPK+TIVTG +SNR G+TTQDTATFIAIG GFLCKSMGFQNTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDKSAFFNCRMDGYQDTLYVQTERQFYRNCVISGTVDFIFGDSTTIIQNSLII 420
           AVALRVQSD+SAFFNCRMDGYQDTLYVQT+RQFYRNCVISGTVDFIFGDSTTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMETTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPRTEAL 540
           VIME+TLGDFI+P GYMPWSGNFALETCLYLEYGNRGPGA TNRRVRWKGVKVI R EA+
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 KFTAGSFLQGGSWLPGTGGPYLLGLK 564
           +FTAG FLQG +WLP TGGPYLLGLK
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLK 566

BLAST of Bhi12G000902 vs. NCBI nr
Match: XP_038875879.1 (LOW QUALITY PROTEIN: pectinesterase-like [Benincasa hispida])

HSP 1 Score: 971.1 bits (2509), Expect = 4.2e-279
Identity = 477/564 (84.57%), Postives = 520/564 (92.20%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVVNKNNNSTTENLSPKMKAVSTICSNTNYQEECQNT 60
           MV KVV+ GISLILVVGVALAVVA+V KNNNS  +  SPKMKAVSTICS T+YQ+ECQNT
Sbjct: 1   MVGKVVVSGISLILVVGVALAVVAMVTKNNNSDVDLSSPKMKAVSTICSTTDYQQECQNT 60

Query: 61  LTNVAHNASSDDPKEYVKAAILATMEEVKKGYNLTDGFMIEAANNASIKMGVEDCKDLLQ 120
           LTNVA NAS DDPKEY KAA+LAT++E+ KGYNLT   M+EAANNASIKM V+DCKDLLQ
Sbjct: 61  LTNVAQNASVDDPKEYAKAAVLATIQEITKGYNLTADLMVEAANNASIKMSVDDCKDLLQ 120

Query: 121 FAIAQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLGEFDPNLQQKMQDG 180
            AI +L ASY+TVG+PDLHTN DRVADIKNWLTAVISYQQSCLDGL EFDP L+QKMQDG
Sbjct: 121 SAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQDG 180

Query: 181 LDIAAKLTSNALAIVDTVSDILQNFGLQFKVQPSGRKLLGTIEVGSDGFPTWMSGADRKL 240
           LD+A KLTSNALAIV  VSDIL+NFGLQ KVQPSGR+LL T E+GSDGFPTWMSGADRKL
Sbjct: 181 LDVAGKLTSNALAIVGAVSDILKNFGLQLKVQPSGRRLLETTEIGSDGFPTWMSGADRKL 240

Query: 241 LASRGGG-RVKPNAVVAKDGSGQFKTIGAALAAYPKALKGRYVIYVKAGIYNEYVTVTKD 300
           LASRGGG RVKPNAVVAKDGSGQ+K+IGAALAAYPKAL+GRYVIYVKAGIY+EY+TVTKD
Sbjct: 241 LASRGGGARVKPNAVVAKDGSGQYKSIGAALAAYPKALRGRYVIYVKAGIYDEYITVTKD 300

Query: 301 MTNIFMYGDGPKRTIVTGRRSNRAGYTTQDTATFIAIGAGFLCKSMGFQNTAGPEGHQAV 360
           M N+FMYGDGP++TIVTGR++NR G TTQ+TA+F AIG GFLCKSMGF+NTAGPEGHQAV
Sbjct: 301 MKNVFMYGDGPRKTIVTGRKNNRDGITTQNTASFSAIGEGFLCKSMGFRNTAGPEGHQAV 360

Query: 361 ALRVQSDKSAFFNCRMDGYQDTLYVQTERQFYRNCVISGTVDFIFGDSTTIIQNSLIIVR 420
           ALRVQSD+SAF+NCRMDGYQDTLYVQT RQFYRNCVISGTVDFIFGDS+TIIQNSLIIVR
Sbjct: 361 ALRVQSDRSAFYNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVR 420

Query: 421 RPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVI 480
           RPMDNQ+NTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYARTVI
Sbjct: 421 RPMDNQKNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVI 480

Query: 481 METTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPRTEALKF 540
           METTLGDFI+PDGYMPWSGNFALETC Y EYGNRGPGANTNRRVRWKGV+VI R EAL++
Sbjct: 481 METTLGDFIQPDGYMPWSGNFALETCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEALQY 540

Query: 541 TAGSFLQGGSWLPGTGGPYLLGLK 564
           TAG FL G +WLP TGGPYLLGLK
Sbjct: 541 TAGPFLMGRAWLPATGGPYLLGLK 564

BLAST of Bhi12G000902 vs. NCBI nr
Match: KGN49779.1 (hypothetical protein Csa_017783 [Cucumis sativus])

HSP 1 Score: 937.6 bits (2422), Expect = 5.1e-269
Identity = 462/565 (81.77%), Postives = 512/565 (90.62%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVVNKNNNS-TTENLSPKMKAVSTICSNTNYQEECQN 60
           MV KVV+ GISLILVVGVALAVVA+VNK+++S  T NLSPKMKAV++ICSN +YQ ECQ 
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60

Query: 61  TLTNVAHNASSDDPKEYVKAAILATMEEVKKGYNLTDGFMIEAANNASIKMGVEDCKDLL 120
           TL +V +N+S +DPKE++KAAI +T+EE+KKGYNLTD  M+EAANNA+IKM V+DCKDLL
Sbjct: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL 120

Query: 121 QFAIAQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLGEFDPNLQQKMQD 180
           Q AI +L ASY+TVG+PDLHTN DR+ADIKNWLTAVISYQQSCLDGL EFDP L+QKMQD
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180

Query: 181 GLDIAAKLTSNALAIVDTVSDILQNFGLQFKVQPSGRKLLGTIEVGSDGFPTWMSGADRK 240
           GLD+A KLTSNALAIV  VSDIL+  GLQ KVQPSGR+LLGT EV SD FPTW++G+DRK
Sbjct: 181 GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK 240

Query: 241 LLAS-RGGGRVKPNAVVAKDGSGQFKTIGAALAAYPKALKGRYVIYVKAGIYNEYVTVTK 300
           LLA+ RGG RVKPN VVAKDGSGQ+KTIGAALAAYPKALKGRYVIYVKAG+YNE + VTK
Sbjct: 241 LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK 300

Query: 301 DMTNIFMYGDGPKRTIVTGRRSNRAGYTTQDTATFIAIGAGFLCKSMGFQNTAGPEGHQA 360
           DM NIFMYGDGP++TIVTGR+SNR G TTQ+TA+F AIG GFLCKSMGF NTAGPEGHQA
Sbjct: 301 DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA 360

Query: 361 VALRVQSDKSAFFNCRMDGYQDTLYVQTERQFYRNCVISGTVDFIFGDSTTIIQNSLIIV 420
           VALRVQSD+SAFFNCRMDGYQDTLYVQT RQFYRNCVISGTVDFIFGDS TIIQNSLIIV
Sbjct: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTV 480
           RRPMDNQQNTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPT+LGRPWK+YARTV
Sbjct: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480

Query: 481 IMETTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPRTEALK 540
           IME+TLGDFI+P GYMPWSGNFAL TC Y EYGNRGPGANTNRRVRWKGV+VI R EA++
Sbjct: 481 IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ 540

Query: 541 FTAGSFLQGGSWLPGTGGPYLLGLK 564
           FTAG FL G +WLPGTGGPYLLGLK
Sbjct: 541 FTAGPFLLGKAWLPGTGGPYLLGLK 565

BLAST of Bhi12G000902 vs. ExPASy TrEMBL
Match: A0A5A7U9J8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00100 PE=3 SV=1)

HSP 1 Score: 1007.3 bits (2603), Expect = 2.5e-290
Identity = 498/566 (87.99%), Postives = 534/566 (94.35%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVV--NKNNNSTTENLSPKMKAVSTICSNTNYQEECQ 60
           MVNKV+LPGISLILVVGVALAVVA+V  NK++++ TE+LSPKMKAVSTIC+N NYQ+ECQ
Sbjct: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLTNVAHNASSDDPKEYVKAAILATMEEVKKGYNLTDGFMIEAANNASIKMGVEDCKDL 120
           NTL+N AHNASSDDPKEYV+AAIL+T+EEVKKGYNLTDGFMIEAANN SIKMGVEDC+DL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIAQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLGEFDPNLQQKMQ 180
           LQFAI QLQASY+TVGEPDLHTNADRVADIKNWLT+VISYQQSCLDGL EFDP L+QKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDIAAKLTSNALAIVDTVSDILQNFGLQFKVQPSGRKLLGTIEVGSDGFPTWMSGADR 240
           DGL  A KLTSNALAIVD VSDIL +FGLQ K QPSGR+LLGT EVG DG+PTW++GADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLASRGGG-RVKPNAVVAKDGSGQFKTIGAALAAYPKALKGRYVIYVKAGIYNEYVTVT 300
           KLLASRGGG +VKPNAVVAKDGSGQFKTI AALAAYPKAL+GRYVIYVKAGIYNEYV +T
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMTNIFMYGDGPKRTIVTGRRSNRAGYTTQDTATFIAIGAGFLCKSMGFQNTAGPEGHQ 360
           KDM NIFMYGDGPK+TIVTG +SNR G+TTQDTATFIAIG GFLCKSMGFQNTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDKSAFFNCRMDGYQDTLYVQTERQFYRNCVISGTVDFIFGDSTTIIQNSLII 420
           AVALRVQSD+SAFFNCRMDGYQDTLYVQT+RQFYRNCVISGTVDFIFGDSTTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMETTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPRTEAL 540
           VIME+TLGDFI+P GYMPWSGNFALETCLYLEYGNRGPGA TNRRVRWKGVKVI R EA+
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 KFTAGSFLQGGSWLPGTGGPYLLGLK 564
           +FTAG FLQG +WLP TGGPYLLGLK
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLK 566

BLAST of Bhi12G000902 vs. ExPASy TrEMBL
Match: A0A1S3CR27 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC107990290 PE=3 SV=1)

HSP 1 Score: 1006.1 bits (2600), Expect = 5.7e-290
Identity = 497/566 (87.81%), Postives = 534/566 (94.35%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVV--NKNNNSTTENLSPKMKAVSTICSNTNYQEECQ 60
           MVNKV+LPGI+LILVVGVALAVVA+V  NK++++ TE+LSPKMKAVSTIC+N NYQ+ECQ
Sbjct: 1   MVNKVILPGITLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLTNVAHNASSDDPKEYVKAAILATMEEVKKGYNLTDGFMIEAANNASIKMGVEDCKDL 120
           NTL+N AHNASSDDPKEYV+AAIL+T+EEVKKGYNLTDGFMIEAANN SIKMGVEDC+DL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIAQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLGEFDPNLQQKMQ 180
           LQFAI QLQASY+TVGEPDLHTNADRVADIKNWLT+VISYQQSCLDGL EFDP L+QKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDIAAKLTSNALAIVDTVSDILQNFGLQFKVQPSGRKLLGTIEVGSDGFPTWMSGADR 240
           DGL  A KLTSNALAIVD VSDIL +FGLQ K QPSGR+LLGT EVG DG+PTW++GADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLASRGGG-RVKPNAVVAKDGSGQFKTIGAALAAYPKALKGRYVIYVKAGIYNEYVTVT 300
           KLLASRGGG +VKPNAVVAKDGSGQFKTI AALAAYPKAL+GRYVIYVKAGIYNEYV +T
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMTNIFMYGDGPKRTIVTGRRSNRAGYTTQDTATFIAIGAGFLCKSMGFQNTAGPEGHQ 360
           KDM NIFMYGDGPK+TIVTG +SNR G+TTQDTATFIAIG GFLCKSMGFQNTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDKSAFFNCRMDGYQDTLYVQTERQFYRNCVISGTVDFIFGDSTTIIQNSLII 420
           AVALRVQSD+SAFFNCRMDGYQDTLYVQT+RQFYRNCVISGTVDFIFGDSTTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMETTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPRTEAL 540
           VIME+TLGDFI+P GYMPWSGNFALETCLYLEYGNRGPGA TNRRVRWKGVKVI R EA+
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 KFTAGSFLQGGSWLPGTGGPYLLGLK 564
           +FTAG FLQG +WLP TGGPYLLGLK
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLK 566

BLAST of Bhi12G000902 vs. ExPASy TrEMBL
Match: A0A0A0KLN2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1)

HSP 1 Score: 937.6 bits (2422), Expect = 2.5e-269
Identity = 462/565 (81.77%), Postives = 512/565 (90.62%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVVNKNNNS-TTENLSPKMKAVSTICSNTNYQEECQN 60
           MV KVV+ GISLILVVGVALAVVA+VNK+++S  T NLSPKMKAV++ICSN +YQ ECQ 
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60

Query: 61  TLTNVAHNASSDDPKEYVKAAILATMEEVKKGYNLTDGFMIEAANNASIKMGVEDCKDLL 120
           TL +V +N+S +DPKE++KAAI +T+EE+KKGYNLTD  M+EAANNA+IKM V+DCKDLL
Sbjct: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL 120

Query: 121 QFAIAQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLGEFDPNLQQKMQD 180
           Q AI +L ASY+TVG+PDLHTN DR+ADIKNWLTAVISYQQSCLDGL EFDP L+QKMQD
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180

Query: 181 GLDIAAKLTSNALAIVDTVSDILQNFGLQFKVQPSGRKLLGTIEVGSDGFPTWMSGADRK 240
           GLD+A KLTSNALAIV  VSDIL+  GLQ KVQPSGR+LLGT EV SD FPTW++G+DRK
Sbjct: 181 GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK 240

Query: 241 LLAS-RGGGRVKPNAVVAKDGSGQFKTIGAALAAYPKALKGRYVIYVKAGIYNEYVTVTK 300
           LLA+ RGG RVKPN VVAKDGSGQ+KTIGAALAAYPKALKGRYVIYVKAG+YNE + VTK
Sbjct: 241 LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK 300

Query: 301 DMTNIFMYGDGPKRTIVTGRRSNRAGYTTQDTATFIAIGAGFLCKSMGFQNTAGPEGHQA 360
           DM NIFMYGDGP++TIVTGR+SNR G TTQ+TA+F AIG GFLCKSMGF NTAGPEGHQA
Sbjct: 301 DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA 360

Query: 361 VALRVQSDKSAFFNCRMDGYQDTLYVQTERQFYRNCVISGTVDFIFGDSTTIIQNSLIIV 420
           VALRVQSD+SAFFNCRMDGYQDTLYVQT RQFYRNCVISGTVDFIFGDS TIIQNSLIIV
Sbjct: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTV 480
           RRPMDNQQNTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPT+LGRPWK+YARTV
Sbjct: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480

Query: 481 IMETTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPRTEALK 540
           IME+TLGDFI+P GYMPWSGNFAL TC Y EYGNRGPGANTNRRVRWKGV+VI R EA++
Sbjct: 481 IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ 540

Query: 541 FTAGSFLQGGSWLPGTGGPYLLGLK 564
           FTAG FL G +WLPGTGGPYLLGLK
Sbjct: 541 FTAGPFLLGKAWLPGTGGPYLLGLK 565

BLAST of Bhi12G000902 vs. ExPASy TrEMBL
Match: A0A5A7U4V7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00110 PE=3 SV=1)

HSP 1 Score: 935.6 bits (2417), Expect = 9.4e-269
Identity = 456/566 (80.57%), Postives = 514/566 (90.81%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVVNKNN--NSTTENLSPKMKAVSTICSNTNYQEECQ 60
           MV KV++  +SLILVVG+ALAVVA +NK N  N   E +SPKMKA+S ICS T+YQEEC+
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  NTLTNVAHNASSDDPKEYVKAAILATMEEVKKGYNLTDGFMIEAANNASIKMGVEDCKDL 120
           NTL +VAHN SS+DPK+Y KAAILAT++E+ KGYNL+D  ++EA NNASIKM VEDCKDL
Sbjct: 61  NTLNHVAHNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCKDL 120

Query: 121 LQFAIAQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLGEFDPNLQQKMQ 180
           LQFAI +LQASY+TVGE DLHT+ADR+ADIKNWL+AV+SYQQSCLDGLGEFDP L+Q+MQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DGLDIAAKLTSNALAIVDTVSDILQNFGLQFKVQPSGRKLLGTIEVGSDGFPTWMSGADR 240
           DGLD+A KLTSNALAIV  VS+IL N+GLQ KVQPSGR+LLGT EV SDGFPTW++GADR
Sbjct: 181 DGLDVAGKLTSNALAIVTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 KLLASRGGG-RVKPNAVVAKDGSGQFKTIGAALAAYPKALKGRYVIYVKAGIYNEYVTVT 300
           KLLAS+ GG R  PNAVVAKDGSGQ+KTI AALAAYPKAL+GRYVIYVKAGIY+EY+TVT
Sbjct: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300

Query: 301 KDMTNIFMYGDGPKRTIVTGRRSNRAGYTTQDTATFIAIGAGFLCKSMGFQNTAGPEGHQ 360
           KDM N+FMYGDGP++TIVTGR+SNR G+TTQ+TA+F AIG GFLCKSMGF NTAGP+GHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360

Query: 361 AVALRVQSDKSAFFNCRMDGYQDTLYVQTERQFYRNCVISGTVDFIFGDSTTIIQNSLII 420
           AVALRVQSD+SAFFNCRMDG+QDTLYVQT RQFYRNCVISGTVDFIFGDS+TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTA GRA+ KE++GLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMETTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPRTEAL 540
           VIME+TLGDFI+P GYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVI R EAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540

Query: 541 KFTAGSFLQGGSWLPGTGGPYLLGLK 564
           ++TAG+FL G +WLP TGG Y LGLK
Sbjct: 541 QYTAGAFLLGRTWLPRTGGIYYLGLK 566

BLAST of Bhi12G000902 vs. ExPASy TrEMBL
Match: A0A5A7U7W4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00090 PE=3 SV=1)

HSP 1 Score: 931.0 bits (2405), Expect = 2.3e-267
Identity = 460/565 (81.42%), Postives = 511/565 (90.44%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVVNKNNNS-TTENLSPKMKAVSTICSNTNYQEECQN 60
           MV KVV+  ++ +LVVGV LAV A+VNKN++S  T ++SPKMKAVS+ICSNT+YQ+ECQ 
Sbjct: 1   MVAKVVM-AVTSLLVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQT 60

Query: 61  TLTNVAHNASSDDPKEYVKAAILATMEEVKKGYNLTDGFMIEAANNASIKMGVEDCKDLL 120
           TL NV HN+S DDPKE++KAAI AT+EE+KKGYNLTD  M+EAANNASIKM V+DC+DLL
Sbjct: 61  TLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLL 120

Query: 121 QFAIAQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLGEFDPNLQQKMQD 180
           Q AI +L ASY+TVG+PDLHTN DRVADIKNWLTAVISYQQSCLDGL EFDP L+QKMQ 
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQG 180

Query: 181 GLDIAAKLTSNALAIVDTVSDILQNFGLQFKVQPSGRKLLGTIEVGSDGFPTWMSGADRK 240
           GLD+AAKLTSNALAIVD VSDIL++ GLQ KVQPSGR+LLGT EV +DG+PTW++GADRK
Sbjct: 181 GLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADRK 240

Query: 241 LLAS-RGGGRVKPNAVVAKDGSGQFKTIGAALAAYPKALKGRYVIYVKAGIYNEYVTVTK 300
           LLA+ RGG RVKPNAVVAKDGSGQ+KTI AALAAYPKALKGRYVIYVKAGIYNEY+ VTK
Sbjct: 241 LLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTK 300

Query: 301 DMTNIFMYGDGPKRTIVTGRRSNRAGYTTQDTATFIAIGAGFLCKSMGFQNTAGPEGHQA 360
           DM N+FMYGDGP++TIVTGR+SNR G  TQ+TATF AIG GFLCKSMGF NTAGPEGHQA
Sbjct: 301 DMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQA 360

Query: 361 VALRVQSDKSAFFNCRMDGYQDTLYVQTERQFYRNCVISGTVDFIFGDSTTIIQNSLIIV 420
           VALRVQSD+SAFFNCRMDGYQDTLYVQT RQFYRNCV+SGTVDFIFGDS TIIQNSLIIV
Sbjct: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTV 480
           RRPMDNQQNTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPT+LGRPWK+YARTV
Sbjct: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480

Query: 481 IMETTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPRTEALK 540
           IMETTLGDFI+P GYMPWSG+FAL TC Y EYGNRGPGANT RRVRWKGV+VI R EA++
Sbjct: 481 IMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQ 540

Query: 541 FTAGSFLQGGSWLPGTGGPYLLGLK 564
           FTAG FL G +WLP TGGPYLLGLK
Sbjct: 541 FTAGPFLLGRAWLPATGGPYLLGLK 564

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G27870.11.5e-13041.89Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G05610.15.1e-12640.43Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G47030.12.0e-12543.93Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G26450.12.2e-12444.74Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G47040.13.4e-12242.58Plant invertase/pectin methylesterase inhibitor superfamily [more]
Match NameE-valueIdentityDescription
Q3E8Z82.2e-12941.89Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3... [more]
Q8GX867.2e-12540.43Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3... [more]
O807222.7e-12443.93Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1[more]
Q7Y2013.0e-12344.74Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3... [more]
Q5MFV84.9e-12142.58Pectinesterase 5 OS=Arabidopsis thaliana OX=3702 GN=PME5 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_038875878.10.0e+00100.00pectinesterase-like [Benincasa hispida][more]
KAA0050275.15.3e-29087.99pectinesterase-like [Cucumis melo var. makuwa] >TYJ98132.1 pectinesterase-like [... [more]
XP_008466374.11.2e-28987.81PREDICTED: pectinesterase-like [Cucumis melo][more]
XP_038875879.14.2e-27984.57LOW QUALITY PROTEIN: pectinesterase-like [Benincasa hispida][more]
KGN49779.15.1e-26981.77hypothetical protein Csa_017783 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A5A7U9J82.5e-29087.99Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00100... [more]
A0A1S3CR275.7e-29087.81Pectinesterase OS=Cucumis melo OX=3656 GN=LOC107990290 PE=3 SV=1[more]
A0A0A0KLN22.5e-26981.77Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1[more]
A0A5A7U4V79.4e-26980.57Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00110... [more]
A0A5A7U7W42.3e-26781.42Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00090... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 39..194
e-value: 3.3E-31
score: 119.7
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 22..197
e-value: 8.4E-16
score: 55.8
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 43..194
e-value: 5.1E-23
score: 82.1
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 252..547
e-value: 2.8E-130
score: 433.4
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 41..199
e-value: 2.9E-28
score: 100.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 42..198
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 246..562
e-value: 1.7E-111
score: 374.7
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 4..562
NoneNo IPR availablePANTHERPTHR31707:SF164PECTINESTERASE 4-RELATEDcoord: 4..562
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 46..199
e-value: 6.14186E-46
score: 156.83
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 396..405
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 249..561

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi12M000902Bhi12M000902mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity