Bhi12G000831 (gene) Wax gourd (B227) v1

Overview
NameBhi12G000831
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionB3 domain-containing protein At2g31420-like
Locationchr12: 28764377 .. 28765120 (+)
RNA-Seq ExpressionBhi12G000831
SyntenyBhi12G000831
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTAAAGGGGGCAAAGGTTGAAGGCTTTAGAATGGGTTTTCGTATTCCTGATTTAAACCAAATTTACATACCGAAGAGAAGAAGATCTTCCGGTGCCGGAAATAAAGAAGAACCTAAGATTGAAACCGATTTTCCAATGTCTATGTCAGAGGCTGCGGATATTCTGGTAGAGATATCACGCTCTGTTTTTTATGTTGAACCAGAAAAAATTAACAAAACCCACAAAAGAAAAAAGCCTTCGCAGCCGACGCCGACCACTATGACTAAGAAACAGATGAACAAGAAGGCTAAAAAGAGCAAGTCTTTGTCGATTCTTGAGATGCCGGCGGCAATGAGAGATCGGATCGTGAAAATGGGTGGCTACGAAATTAATCTGGTGATTCAGAAACAACTCCAAGATACGGATCTAAACAAAAATCAAGGGCGTTTGTCCCTCCCAACAGAGAAATTGTTGTTCGATTTTGCAACAGAGGAGGAGAAGAAATTGCTAAGCCAACGAGAAAATAAAAAGAAGAAAGGGATGAATGTATTGATAATGGATAATGTTTCTGAAGAAAGAAGGATTTGCTTGAAGAAGTGGAAGATTGGAAGTGGGGATGTGTATTGTTTGATGACACAATGGAATGCGTTTGCGGAAGAAACGGGATTGAAAAGTGGACAACATATTCAAATTTGGTGCTTTAGAAGAGATGATGAATTTGAAGCTTCTCGTCTTTGCTTTGGTATGGTTAAGTTAAACTAG

mRNA sequence

ATGGTAAAGGGGGCAAAGGTTGAAGGCTTTAGAATGGGTTTTCGTATTCCTGATTTAAACCAAATTTACATACCGAAGAGAAGAAGATCTTCCGGTGCCGGAAATAAAGAAGAACCTAAGATTGAAACCGATTTTCCAATGTCTATGTCAGAGGCTGCGGATATTCTGGTAGAGATATCACGCTCTGTTTTTTATGTTGAACCAGAAAAAATTAACAAAACCCACAAAAGAAAAAAGCCTTCGCAGCCGACGCCGACCACTATGACTAAGAAACAGATGAACAAGAAGGCTAAAAAGAGCAAGTCTTTGTCGATTCTTGAGATGCCGGCGGCAATGAGAGATCGGATCGTGAAAATGGGTGGCTACGAAATTAATCTGGTGATTCAGAAACAACTCCAAGATACGGATCTAAACAAAAATCAAGGGCGTTTGTCCCTCCCAACAGAGAAATTGTTGTTCGATTTTGCAACAGAGGAGGAGAAGAAATTGCTAAGCCAACGAGAAAATAAAAAGAAGAAAGGGATGAATGTATTGATAATGGATAATGTTTCTGAAGAAAGAAGGATTTGCTTGAAGAAGTGGAAGATTGGAAGTGGGGATGTGTATTGTTTGATGACACAATGGAATGCGTTTGCGGAAGAAACGGGATTGAAAAGTGGACAACATATTCAAATTTGGTGCTTTAGAAGAGATGATGAATTTGAAGCTTCTCGTCTTTGCTTTGGTATGGTTAAGTTAAACTAG

Coding sequence (CDS)

ATGGTAAAGGGGGCAAAGGTTGAAGGCTTTAGAATGGGTTTTCGTATTCCTGATTTAAACCAAATTTACATACCGAAGAGAAGAAGATCTTCCGGTGCCGGAAATAAAGAAGAACCTAAGATTGAAACCGATTTTCCAATGTCTATGTCAGAGGCTGCGGATATTCTGGTAGAGATATCACGCTCTGTTTTTTATGTTGAACCAGAAAAAATTAACAAAACCCACAAAAGAAAAAAGCCTTCGCAGCCGACGCCGACCACTATGACTAAGAAACAGATGAACAAGAAGGCTAAAAAGAGCAAGTCTTTGTCGATTCTTGAGATGCCGGCGGCAATGAGAGATCGGATCGTGAAAATGGGTGGCTACGAAATTAATCTGGTGATTCAGAAACAACTCCAAGATACGGATCTAAACAAAAATCAAGGGCGTTTGTCCCTCCCAACAGAGAAATTGTTGTTCGATTTTGCAACAGAGGAGGAGAAGAAATTGCTAAGCCAACGAGAAAATAAAAAGAAGAAAGGGATGAATGTATTGATAATGGATAATGTTTCTGAAGAAAGAAGGATTTGCTTGAAGAAGTGGAAGATTGGAAGTGGGGATGTGTATTGTTTGATGACACAATGGAATGCGTTTGCGGAAGAAACGGGATTGAAAAGTGGACAACATATTCAAATTTGGTGCTTTAGAAGAGATGATGAATTTGAAGCTTCTCGTCTTTGCTTTGGTATGGTTAAGTTAAACTAG

Protein sequence

MVKGAKVEGFRMGFRIPDLNQIYIPKRRRSSGAGNKEEPKIETDFPMSMSEAADILVEISRSVFYVEPEKINKTHKRKKPSQPTPTTMTKKQMNKKAKKSKSLSILEMPAAMRDRIVKMGGYEINLVIQKQLQDTDLNKNQGRLSLPTEKLLFDFATEEEKKLLSQRENKKKKGMNVLIMDNVSEERRICLKKWKIGSGDVYCLMTQWNAFAEETGLKSGQHIQIWCFRRDDEFEASRLCFGMVKLN
Homology
BLAST of Bhi12G000831 vs. TAIR 10
Match: AT2G31420.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 68.6 bits (166), Expect = 8.3e-12
Identity = 46/129 (35.66%), Postives = 69/129 (53.49%), Query Frame = 0

Query: 118 KMGGYEINLVIQKQ-LQDTDLNKNQGRLSLPTEKL-LFDFATEEEKKLLSQRENK-KKKG 177
           K  GY+  L+I+++ L  TDL KNQG LS+   KL   DF TE+E + L +   K K+ G
Sbjct: 70  KENGYDPKLIIKRRVLCTTDLRKNQGCLSMHLSKLEKSDFLTEDETRFLDEDFLKAKRDG 129

Query: 178 MNVLIMDNVSEERRICLKKWKIGSGDVYCLMTQWNAFAEETGLKSGQHIQIWCFRRDDEF 237
           + V ++D  S++  + LKKW   +   Y L   WN   ++   K    I+IW FR     
Sbjct: 130 LKVFLVDPESDKHVVYLKKWNGNTVWKYVLSHGWNNVIDKKIFKVNDVIEIWSFRDG--- 189

Query: 238 EASRLCFGM 244
            + +LCF +
Sbjct: 190 -SGKLCFAL 194

BLAST of Bhi12G000831 vs. TAIR 10
Match: AT3G24850.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 65.5 bits (158), Expect = 7.0e-11
Identity = 58/189 (30.69%), Postives = 88/189 (46.56%), Query Frame = 0

Query: 78  KKPSQP-----TPTTMTKKQMNKKAKKS---KSLSILEMPAAMRDR---IVKM-----GG 137
           K P  P     T  T  K++  ++ K++   K   +   P   RD    +VK+       
Sbjct: 164 KDPPNPLSLCLTGNTSRKRRAVEERKRTGGFKKAKVAPFPGTARDTPKWLVKVMRDMKEA 223

Query: 138 YEINLVIQKQLQDTDLNKNQGRLSLPTEKLL-FDFATEEEKKLLSQR-ENKKKKGMNVLI 197
            +  L+ +K L  TD+N  Q RLS+P   LL  DF T  E +++ +   N KK G+  ++
Sbjct: 224 KDAKLIFEKTLFVTDINPTQNRLSMPFNNLLQNDFLTSVESRIIKEDINNNKKIGVGAIL 283

Query: 198 MDNVSEERRICLKKWKI----GSGD-VYCLMTQWNAFAEETGLKSGQHIQIWCFRRDDEF 244
           +D  S +  + LK+W++    G G   Y L+  WN   E  GLK G +I +W FR     
Sbjct: 284 VDQRSVKWGVMLKRWELKKESGKGSWNYNLICGWNDVVEANGLKEGDNISVWSFRC---- 343

BLAST of Bhi12G000831 vs. TAIR 10
Match: AT1G05920.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 63.5 bits (153), Expect = 2.7e-10
Identity = 41/133 (30.83%), Postives = 67/133 (50.38%), Query Frame = 0

Query: 120 GGYEINLVIQKQLQDTDLNKNQGRLSLPTEKLL-FDFATEEEKKLLSQRE--NKKKKGMN 179
           G  +  L+I+K L   D++  Q RLS+P   ++  DF T +E +L+ + E  N+   G+ 
Sbjct: 176 GAGDPKLIIEKNLDSNDVDPRQNRLSIPINTVIQNDFLTLDESRLIDEDEITNEGNMGVA 235

Query: 180 VLIMDNVSEERRICLKKW--KIGSGDVY---CLMTQWNAFAEETGLKSGQHIQIWCFRRD 239
             ++D  +++  +  K+W     SG  Y    L  +W+   E  GLK G  I +W FR +
Sbjct: 236 AFLVDQRTKKWNMGFKQWFMTTDSGSSYWSFVLRGEWSNVVETNGLKEGDKISLWSFRSN 295

Query: 240 DEFEASRLCFGMV 245
           D      LCF +V
Sbjct: 296 D-----ILCFALV 303

BLAST of Bhi12G000831 vs. TAIR 10
Match: AT2G31720.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 63.5 bits (153), Expect = 2.7e-10
Identity = 35/123 (28.46%), Postives = 67/123 (54.47%), Query Frame = 0

Query: 114 DRIVKMGGYEINLVIQKQLQDTDLNKNQGRLSLPTEKLL-FDFATEEEKKLLSQRENK-- 173
           D + ++ G +  L+  K L ++D+++ Q RL +P +++L  DF  EEE + + +   K  
Sbjct: 135 DVMREVNGMDAKLIFVKVLPNSDVDELQTRLMMPWKQILDMDFLNEEELEKIDRHHKKIS 194

Query: 174 -KKKGMNVLIMDNVSEERRICLKKWKIGSGDVYCLMTQWNAFAEETGLKSGQHIQIWCFR 233
              KG +V+++++   +R++ LK+W + S   Y L   WN    +  L+ G  ++IW F 
Sbjct: 195 ASDKGADVIVVNSKGLQRKLKLKRWDMTSTSNYVLGLGWNKVVTDNILQRGTRLRIWSFH 254

BLAST of Bhi12G000831 vs. TAIR 10
Match: AT2G24670.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 52.8 bits (125), Expect = 4.7e-07
Identity = 41/166 (24.70%), Postives = 84/166 (50.60%), Query Frame = 0

Query: 77  RKKPSQPTPTTMTKKQMNKKAKKSKSLSILEMPAAMRDRIVKM------GGYEIN--LVI 136
           R +   P  +   +++ +KKAK+S   S  +        ++ +      G  E N   +I
Sbjct: 74  RNRHDLPPMSPPMEQRKSKKAKRSTDASSSKTREPTPGWLISLMRSKNGGDVEDNSKKII 133

Query: 137 QKQLQDTDLNKNQGRLSLPTEKLL-FDFATEEEKKLLSQRENK-KKKGMNVLIMDNVSEE 196
            K+L  TD++ +Q RLS+P  +++  +F   EEK+ + +  N+ +K+G++ +++D+   E
Sbjct: 134 DKELFQTDVDPHQSRLSIPVSQIVELEFLNHEEKRAIEEDANRVRKEGVDAILVDSHLRE 193

Query: 197 RRICLKKWKIGSGDVYCLMTQWNAFAEETGLKSGQHIQIWCFRRDD 233
             + L+   +    +Y L+  WN    +  L+   +I++W F  DD
Sbjct: 194 FPVNLRLRDMRGILLYNLVAGWNQVVTDCLLEENTNIRLWSFHADD 239

BLAST of Bhi12G000831 vs. ExPASy Swiss-Prot
Match: Q9SIC7 (B3 domain-containing protein At2g31420 OS=Arabidopsis thaliana OX=3702 GN=At2g31420 PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.2e-10
Identity = 46/129 (35.66%), Postives = 69/129 (53.49%), Query Frame = 0

Query: 118 KMGGYEINLVIQKQ-LQDTDLNKNQGRLSLPTEKL-LFDFATEEEKKLLSQRENK-KKKG 177
           K  GY+  L+I+++ L  TDL KNQG LS+   KL   DF TE+E + L +   K K+ G
Sbjct: 70  KENGYDPKLIIKRRVLCTTDLRKNQGCLSMHLSKLEKSDFLTEDETRFLDEDFLKAKRDG 129

Query: 178 MNVLIMDNVSEERRICLKKWKIGSGDVYCLMTQWNAFAEETGLKSGQHIQIWCFRRDDEF 237
           + V ++D  S++  + LKKW   +   Y L   WN   ++   K    I+IW FR     
Sbjct: 130 LKVFLVDPESDKHVVYLKKWNGNTVWKYVLSHGWNNVIDKKIFKVNDVIEIWSFRDG--- 189

Query: 238 EASRLCFGM 244
            + +LCF +
Sbjct: 190 -SGKLCFAL 194

BLAST of Bhi12G000831 vs. ExPASy Swiss-Prot
Match: Q9LRX6 (Putative B3 domain-containing protein At3g24850 OS=Arabidopsis thaliana OX=3702 GN=At3g24850 PE=3 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 9.9e-10
Identity = 58/189 (30.69%), Postives = 88/189 (46.56%), Query Frame = 0

Query: 78  KKPSQP-----TPTTMTKKQMNKKAKKS---KSLSILEMPAAMRDR---IVKM-----GG 137
           K P  P     T  T  K++  ++ K++   K   +   P   RD    +VK+       
Sbjct: 164 KDPPNPLSLCLTGNTSRKRRAVEERKRTGGFKKAKVAPFPGTARDTPKWLVKVMRDMKEA 223

Query: 138 YEINLVIQKQLQDTDLNKNQGRLSLPTEKLL-FDFATEEEKKLLSQR-ENKKKKGMNVLI 197
            +  L+ +K L  TD+N  Q RLS+P   LL  DF T  E +++ +   N KK G+  ++
Sbjct: 224 KDAKLIFEKTLFVTDINPTQNRLSMPFNNLLQNDFLTSVESRIIKEDINNNKKIGVGAIL 283

Query: 198 MDNVSEERRICLKKWKI----GSGD-VYCLMTQWNAFAEETGLKSGQHIQIWCFRRDDEF 244
           +D  S +  + LK+W++    G G   Y L+  WN   E  GLK G +I +W FR     
Sbjct: 284 VDQRSVKWGVMLKRWELKKESGKGSWNYNLICGWNDVVEANGLKEGDNISVWSFRC---- 343

BLAST of Bhi12G000831 vs. ExPASy Swiss-Prot
Match: Q9LSF7 (B3 domain-containing protein At3g25182 OS=Arabidopsis thaliana OX=3702 GN=At3g25182 PE=2 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.9e-09
Identity = 55/179 (30.73%), Postives = 83/179 (46.37%), Query Frame = 0

Query: 72  NKTHKRKKPSQPTPTTMTKKQMNKKAKKSKSLSILEMPAAMRDRIVKMGGYEINLVIQKQ 131
           NK  KR+   Q   T   KK       ++   +   +   MRD        +  L+ +K 
Sbjct: 164 NKNRKRRAVEQRKRTGGVKKAKVAPFSQTARETPEWLVNVMRD---MKEAKDAKLIFEKT 223

Query: 132 LQDTDLNKNQGRLSLPTEKLL-FDFATEEEKKLLSQR-ENKKKKGMNVLIMDNVSEERRI 191
           L  TD+N  Q RLS+P   LL  DF T  E +++ +  +N KK G+  +++D   ++  +
Sbjct: 224 LFVTDVNPTQNRLSMPFNNLLRNDFLTSVESRIIDKDIKNDKKIGVGAILVDQRCKKWGV 283

Query: 192 CLKKWKI----GSGD-VYCLMTQWNAFAEETGLKSGQHIQIWCFRRDDEFEASRLCFGM 244
            LK+W++    G G   Y L+  WN   E  GLK G +I +W FR         LCF M
Sbjct: 284 MLKRWEMKKESGKGSWNYNLICGWNDIVEANGLKEGDNISLWSFR-----SCGILCFAM 334

BLAST of Bhi12G000831 vs. ExPASy Swiss-Prot
Match: Q5RM09 (B3 domain-containing protein At1g05920 OS=Arabidopsis thaliana OX=3702 GN=At1g05920 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.7e-09
Identity = 41/133 (30.83%), Postives = 67/133 (50.38%), Query Frame = 0

Query: 120 GGYEINLVIQKQLQDTDLNKNQGRLSLPTEKLL-FDFATEEEKKLLSQRE--NKKKKGMN 179
           G  +  L+I+K L   D++  Q RLS+P   ++  DF T +E +L+ + E  N+   G+ 
Sbjct: 176 GAGDPKLIIEKNLDSNDVDPRQNRLSIPINTVIQNDFLTLDESRLIDEDEITNEGNMGVA 235

Query: 180 VLIMDNVSEERRICLKKW--KIGSGDVY---CLMTQWNAFAEETGLKSGQHIQIWCFRRD 239
             ++D  +++  +  K+W     SG  Y    L  +W+   E  GLK G  I +W FR +
Sbjct: 236 AFLVDQRTKKWNMGFKQWFMTTDSGSSYWSFVLRGEWSNVVETNGLKEGDKISLWSFRSN 295

Query: 240 DEFEASRLCFGMV 245
           D      LCF +V
Sbjct: 296 D-----ILCFALV 303

BLAST of Bhi12G000831 vs. ExPASy Swiss-Prot
Match: Q8RV83 (B3 domain-containing protein At2g31720 OS=Arabidopsis thaliana OX=3702 GN=ARF70 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.7e-09
Identity = 35/123 (28.46%), Postives = 67/123 (54.47%), Query Frame = 0

Query: 114 DRIVKMGGYEINLVIQKQLQDTDLNKNQGRLSLPTEKLL-FDFATEEEKKLLSQRENK-- 173
           D + ++ G +  L+  K L ++D+++ Q RL +P +++L  DF  EEE + + +   K  
Sbjct: 135 DVMREVNGMDAKLIFVKVLPNSDVDELQTRLMMPWKQILDMDFLNEEELEKIDRHHKKIS 194

Query: 174 -KKKGMNVLIMDNVSEERRICLKKWKIGSGDVYCLMTQWNAFAEETGLKSGQHIQIWCFR 233
              KG +V+++++   +R++ LK+W + S   Y L   WN    +  L+ G  ++IW F 
Sbjct: 195 ASDKGADVIVVNSKGLQRKLKLKRWDMTSTSNYVLGLGWNKVVTDNILQRGTRLRIWSFH 254

BLAST of Bhi12G000831 vs. NCBI nr
Match: XP_038874962.1 (putative B3 domain-containing protein At5g35780 [Benincasa hispida])

HSP 1 Score: 490.0 bits (1260), Expect = 1.2e-134
Identity = 247/247 (100.00%), Postives = 247/247 (100.00%), Query Frame = 0

Query: 1   MVKGAKVEGFRMGFRIPDLNQIYIPKRRRSSGAGNKEEPKIETDFPMSMSEAADILVEIS 60
           MVKGAKVEGFRMGFRIPDLNQIYIPKRRRSSGAGNKEEPKIETDFPMSMSEAADILVEIS
Sbjct: 1   MVKGAKVEGFRMGFRIPDLNQIYIPKRRRSSGAGNKEEPKIETDFPMSMSEAADILVEIS 60

Query: 61  RSVFYVEPEKINKTHKRKKPSQPTPTTMTKKQMNKKAKKSKSLSILEMPAAMRDRIVKMG 120
           RSVFYVEPEKINKTHKRKKPSQPTPTTMTKKQMNKKAKKSKSLSILEMPAAMRDRIVKMG
Sbjct: 61  RSVFYVEPEKINKTHKRKKPSQPTPTTMTKKQMNKKAKKSKSLSILEMPAAMRDRIVKMG 120

Query: 121 GYEINLVIQKQLQDTDLNKNQGRLSLPTEKLLFDFATEEEKKLLSQRENKKKKGMNVLIM 180
           GYEINLVIQKQLQDTDLNKNQGRLSLPTEKLLFDFATEEEKKLLSQRENKKKKGMNVLIM
Sbjct: 121 GYEINLVIQKQLQDTDLNKNQGRLSLPTEKLLFDFATEEEKKLLSQRENKKKKGMNVLIM 180

Query: 181 DNVSEERRICLKKWKIGSGDVYCLMTQWNAFAEETGLKSGQHIQIWCFRRDDEFEASRLC 240
           DNVSEERRICLKKWKIGSGDVYCLMTQWNAFAEETGLKSGQHIQIWCFRRDDEFEASRLC
Sbjct: 181 DNVSEERRICLKKWKIGSGDVYCLMTQWNAFAEETGLKSGQHIQIWCFRRDDEFEASRLC 240

Query: 241 FGMVKLN 248
           FGMVKLN
Sbjct: 241 FGMVKLN 247

BLAST of Bhi12G000831 vs. NCBI nr
Match: XP_038874960.1 (B3 domain-containing protein At2g31420-like [Benincasa hispida])

HSP 1 Score: 416.4 bits (1069), Expect = 1.7e-112
Identity = 214/238 (89.92%), Postives = 222/238 (93.28%), Query Frame = 0

Query: 12  MGFRIPDLNQIYIPKRRRSSGAGNKEEPKIETDFPMSMSEAADILVEISRSVFYVEPEKI 71
           MGF IPDLNQIYIPKRRRSSGAGNKE+P+IETDFPMSMSEAAD+LVE+S SVF  EPEKI
Sbjct: 1   MGFHIPDLNQIYIPKRRRSSGAGNKEQPEIETDFPMSMSEAADVLVEMSCSVFEAEPEKI 60

Query: 72  NKTHKRKK--PSQPTPTTMTKKQMNKKAKKSKSLSILEMPAAMRDRIVKMGGYEINLVIQ 131
           NKT+KRKK     PTPTTMT+KQMNKKAKKSKS SI E+PAAMRDRIVKMGGY INLVIQ
Sbjct: 61  NKTYKRKKHPTPMPTPTTMTEKQMNKKAKKSKSPSIPEIPAAMRDRIVKMGGYGINLVIQ 120

Query: 132 KQLQDTDLNKNQGRLSLPTEKLLFDFATEEEKKLLSQRENKKKKGMNVLIMDNVSEERRI 191
           KQLQDTDLNKNQGRLSLP EKLLFDFATEEEKKLLSQRENKKKKGMNVLIMDNVSEER I
Sbjct: 121 KQLQDTDLNKNQGRLSLPAEKLLFDFATEEEKKLLSQRENKKKKGMNVLIMDNVSEERSI 180

Query: 192 CLKKWKIGSGDVYCLMTQWNAFAEETGLKSGQHIQIWCFRRDDEFEASRLCFGMVKLN 248
           CLKKWKIGSGDVYCLMTQWNAFAEE GLKSGQHIQIWCFRRDDEFEASRLCF MVKL+
Sbjct: 181 CLKKWKIGSGDVYCLMTQWNAFAEEKGLKSGQHIQIWCFRRDDEFEASRLCFVMVKLD 238

BLAST of Bhi12G000831 vs. NCBI nr
Match: XP_038876237.1 (putative B3 domain-containing protein At3g24850 [Benincasa hispida])

HSP 1 Score: 374.0 bits (959), Expect = 9.9e-100
Identity = 191/242 (78.93%), Postives = 210/242 (86.78%), Query Frame = 0

Query: 7   VEGFRMGFRIPDLNQIYIPKRRR-SSGAGNKEEPKIETDFPMSMSEAADILVEISRSVFY 66
           VE FRMG  +PDLN+IYIP+RRR SSGAG KE+PK ET+ P+++ EAA IL+E++  V  
Sbjct: 7   VESFRMGLDLPDLNEIYIPRRRRGSSGAGKKEQPKTETELPLTVLEAAAILIEMANFVSD 66

Query: 67  VEPEKINKTHKRKKPSQPTPTTMTKKQMNKKAKKSKSLSILEMPAAMRDRIVKMGGYEIN 126
           VEP+ IN THKRKKP  PTPTTMTKKQ  KKAK+SK   I EMP AMRDRIV+MGGY IN
Sbjct: 67  VEPQNIN-THKRKKPPTPTPTTMTKKQRKKKAKQSKYPWITEMPVAMRDRIVEMGGYGIN 126

Query: 127 LVIQKQLQDTDLNKNQGRLSLPTEKLLFDFATEEEKKLLSQRENKKKKGMNVLIMDNVSE 186
           LVIQKQLQDTDLNKNQGRLSLP EKLLFDF TE+EK LLSQRENKKKKGMNVLIMD+V E
Sbjct: 127 LVIQKQLQDTDLNKNQGRLSLPAEKLLFDFTTEDEKILLSQRENKKKKGMNVLIMDDVLE 186

Query: 187 ERRICLKKWKIGSGDVYCLMTQWNAFAEETGLKSGQHIQIWCFRRDDEFEASRLCFGMVK 246
           ERRICLK WKIGSGDVYCLMTQWN+F EETGLKSG+HIQIWCFR+DDEFEASRLCF MVK
Sbjct: 187 ERRICLKMWKIGSGDVYCLMTQWNSFVEETGLKSGEHIQIWCFRKDDEFEASRLCFAMVK 246

Query: 247 LN 248
           LN
Sbjct: 247 LN 247

BLAST of Bhi12G000831 vs. NCBI nr
Match: XP_038907053.1 (B3 domain-containing protein At2g31420-like [Benincasa hispida])

HSP 1 Score: 318.5 bits (815), Expect = 4.9e-83
Identity = 162/246 (65.85%), Postives = 195/246 (79.27%), Query Frame = 0

Query: 1   MVKGAKVEGFRMGFRIPDLNQIYIPKRRRSSGAGNKEEPKIETDFPMSMSEAADILVEIS 60
           M  G  +E FRMGF +PDL+ IYIP++RR S    K++P+ E++ P++M EAA IL+E++
Sbjct: 1   MGMGPAIEAFRMGFDLPDLDNIYIPRKRRGSSGAGKKQPETESELPLTMLEAATILIEMT 60

Query: 61  RSVFYVEPEKINKTHKRKKPSQPTPTTMTKKQMNKKAKKSKSLSILEMPAAMRDRIVKMG 120
            SV  V PEKI+ THKRK P   TP T TKKQ  KKAK+SK  SI EMP A+RDRIV+MG
Sbjct: 61  NSVSDVNPEKISNTHKRKNPL--TPKTATKKQRKKKAKQSKYPSIPEMPEALRDRIVEMG 120

Query: 121 GYEINLVIQKQLQDTDLNKNQGRLSLPTEKLLFDFATEEEKKLLSQRENKKKKGMNVLIM 180
           GYEI LVIQKQL+DTDL+KN GRLS+P +KLLFDF TEEE+KLLS+ EN+ KKG+N +I+
Sbjct: 121 GYEIRLVIQKQLEDTDLSKNHGRLSMPIKKLLFDFTTEEERKLLSEVENQNKKGINTVII 180

Query: 181 DNVSEERRICLKKWKIGSGDVYCLMTQWNAFAEETGLKSGQHIQIWCFRRDDEFEASRLC 240
           D+V EER ICLKKWKIGSGDVYCL TQWN+  EETGLK GQHIQIW FR+DDE EA  +C
Sbjct: 181 DDV-EERMICLKKWKIGSGDVYCLTTQWNSLVEETGLKVGQHIQIWSFRKDDENEAYHVC 240

Query: 241 FGMVKL 247
           F MVKL
Sbjct: 241 FAMVKL 243

BLAST of Bhi12G000831 vs. NCBI nr
Match: XP_008467039.1 (PREDICTED: putative B3 domain-containing protein At3g24850 [Cucumis melo])

HSP 1 Score: 305.4 bits (781), Expect = 4.3e-79
Identity = 159/244 (65.16%), Postives = 193/244 (79.10%), Query Frame = 0

Query: 7   VEGFRMGFRIPDLNQIYIPKRRRSSGAGNKEEPKIETDFPMSMSEAADILVEISRSVFYV 66
           VEGFRM   +   NQ Y+P+RRRS     KE+   +T  P+SMSEAAD+L+E++ SV  V
Sbjct: 7   VEGFRM--NVHSFNQFYVPQRRRSFRVEIKEQSNPQTHLPISMSEAADVLIEMANSVPNV 66

Query: 67  EPEKINKTHKRKKPSQPTPTTMTKKQMNKKAKKSKSLSILEMPAAMRDRIVKMGGYEINL 126
           EP++INKTHKRK P + T T + K++  KK ++S+  SI EMP  MRDRI +MGGYEINL
Sbjct: 67  EPQQINKTHKRKNPPR-TNTAIKKQRRKKKVEQSEYPSIPEMPTTMRDRIEEMGGYEINL 126

Query: 127 VIQKQLQDTDLNKNQGRLSLPTEKLLFDFATEEEKKLLSQRENKKKKGMNVLIMDNVSEE 186
           VIQKQL DTDLNKN+GRLS+P +KL FDF TEEE+ LLSQ+ENK KKG+NV+IM+N+ E+
Sbjct: 127 VIQKQLTDTDLNKNEGRLSMPKKKLAFDFTTEEERNLLSQQENKNKKGINVMIMNNMVED 186

Query: 187 -RRICLKKWKIGS--GDVYCLMTQWNAFAEETGLKSGQHIQIWCFRRDDEFEASRLCFGM 246
            R ICLKKWKIGS  GDVYCLMTQWNAF EETGLKSGQHIQ+WCFR+D EFE  RLCF +
Sbjct: 187 IRSICLKKWKIGSGNGDVYCLMTQWNAFVEETGLKSGQHIQLWCFRKDYEFEPPRLCFAV 246

Query: 247 VKLN 248
           V+LN
Sbjct: 247 VRLN 247

BLAST of Bhi12G000831 vs. ExPASy TrEMBL
Match: A0A1S3CSS4 (putative B3 domain-containing protein At3g24850 OS=Cucumis melo OX=3656 GN=LOC103504467 PE=4 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 2.1e-79
Identity = 159/244 (65.16%), Postives = 193/244 (79.10%), Query Frame = 0

Query: 7   VEGFRMGFRIPDLNQIYIPKRRRSSGAGNKEEPKIETDFPMSMSEAADILVEISRSVFYV 66
           VEGFRM   +   NQ Y+P+RRRS     KE+   +T  P+SMSEAAD+L+E++ SV  V
Sbjct: 7   VEGFRM--NVHSFNQFYVPQRRRSFRVEIKEQSNPQTHLPISMSEAADVLIEMANSVPNV 66

Query: 67  EPEKINKTHKRKKPSQPTPTTMTKKQMNKKAKKSKSLSILEMPAAMRDRIVKMGGYEINL 126
           EP++INKTHKRK P + T T + K++  KK ++S+  SI EMP  MRDRI +MGGYEINL
Sbjct: 67  EPQQINKTHKRKNPPR-TNTAIKKQRRKKKVEQSEYPSIPEMPTTMRDRIEEMGGYEINL 126

Query: 127 VIQKQLQDTDLNKNQGRLSLPTEKLLFDFATEEEKKLLSQRENKKKKGMNVLIMDNVSEE 186
           VIQKQL DTDLNKN+GRLS+P +KL FDF TEEE+ LLSQ+ENK KKG+NV+IM+N+ E+
Sbjct: 127 VIQKQLTDTDLNKNEGRLSMPKKKLAFDFTTEEERNLLSQQENKNKKGINVMIMNNMVED 186

Query: 187 -RRICLKKWKIGS--GDVYCLMTQWNAFAEETGLKSGQHIQIWCFRRDDEFEASRLCFGM 246
            R ICLKKWKIGS  GDVYCLMTQWNAF EETGLKSGQHIQ+WCFR+D EFE  RLCF +
Sbjct: 187 IRSICLKKWKIGSGNGDVYCLMTQWNAFVEETGLKSGQHIQLWCFRKDYEFEPPRLCFAV 246

Query: 247 VKLN 248
           V+LN
Sbjct: 247 VRLN 247

BLAST of Bhi12G000831 vs. ExPASy TrEMBL
Match: A0A5A7TY94 (Putative B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold426G00280 PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 1.0e-73
Identity = 150/256 (58.59%), Postives = 189/256 (73.83%), Query Frame = 0

Query: 9   GFRMGFRIPDLNQIYIPKRRR--SSGAGNKEEPKIETDFPMSMSEAADILVEISRSVFYV 68
           G R+  ++PDLN IYIP++RR  SSGAG KE    ET+ P+SM+EAA IL++++ SV  V
Sbjct: 10  GLRICLQLPDLNDIYIPRKRRGSSSGAGKKERSMTETELPLSMAEAASILIQLANSVPDV 69

Query: 69  EPEKINKTHKRKKPSQPT--PTTMTKKQMN------------KKAKKS--KSLSILEMPA 128
           + +K+NKTHKRK P   T   T+ TKKQ N            KK +K   K +    MP 
Sbjct: 70  QRQKLNKTHKRKNPPTLTEASTSRTKKQRNPPTLTEASTSRTKKQRKKYVKQIEHPSMPV 129

Query: 129 AMRDRIVKMGGYEINLVIQKQLQDTDLNKNQGRLSLPTEKLLFDFATEEEKKLLSQRENK 188
           AMRDRI++MGGYEI LVIQKQL DTDLNKN GRLS+ T++L FDFATEEE KLLS++ENK
Sbjct: 130 AMRDRILEMGGYEIRLVIQKQLTDTDLNKNHGRLSMNTKQLSFDFATEEETKLLSEQENK 189

Query: 189 KKKGMNVLIMDNVSEERRICLKKWKIGSGDVYCLMTQWNAFAEETGLKSGQHIQIWCFRR 247
            K+G+NV+++D+V EER +CLKKWKIGSG+VYCLMT+WN   EE G K G+ IQ+W FR+
Sbjct: 190 NKRGINVMMLDDVLEERMLCLKKWKIGSGEVYCLMTRWNLMVEERGFKGGEEIQVWSFRK 249

BLAST of Bhi12G000831 vs. ExPASy TrEMBL
Match: A0A5A7TWX2 (Putative B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold663G00300 PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 1.0e-73
Identity = 146/242 (60.33%), Postives = 187/242 (77.27%), Query Frame = 0

Query: 9   GFRMGFRIPDLNQIYIPKRRR--SSGAGNKEEPKIETDFPMSMSEAADILVEISRSVFYV 68
           G R+   +PDL+ IYIP++RR  SSGAG KE+   ET+ P+SM+EAA IL++++ SV  V
Sbjct: 10  GLRICHHLPDLDNIYIPRKRRGSSSGAGKKEQSITETELPLSMAEAASILIQLANSVPDV 69

Query: 69  EPEKINKTHKRKKPSQPT--PTTMTKKQMNKKAKKSKSLSILEMPAAMRDRIVKMGGYEI 128
           + +K+NKTHKRK P  PT   T+ TKKQ  K   +S+   I  MP  MRDRI++MGGYEI
Sbjct: 70  QRQKLNKTHKRKNPPIPTEASTSKTKKQRKKNVDQSE---IPSMPVTMRDRILEMGGYEI 129

Query: 129 NLVIQKQLQDTDLNKNQGRLSLPTEKLLFDFATEEEKKLLSQRENKKKKGMNVLIMDNVS 188
            LVIQKQL DTDLNKN GRLS+  ++L FDFATEEE +LLS++ENK KKG+NV+I+D+V 
Sbjct: 130 RLVIQKQLTDTDLNKNHGRLSMNKKQLSFDFATEEESELLSEQENKNKKGINVMILDDVL 189

Query: 189 EERRICLKKWKIGSGDVYCLMTQWNAFAEETGLKSGQHIQIWCFRRDDEFEASRLCFGMV 247
           EER +CLKKWKIGSG+VYCLMT+WN   E+ G +SG+ IQ+W FR+DDE+EA RLCF +V
Sbjct: 190 EERMLCLKKWKIGSGEVYCLMTKWNLMVEKRGFESGEEIQVWSFRKDDEYEAHRLCFALV 248

BLAST of Bhi12G000831 vs. ExPASy TrEMBL
Match: A0A1S4E600 (putative B3 domain-containing protein At3g24850 OS=Cucumis melo OX=3656 GN=LOC103504562 PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 1.0e-73
Identity = 150/256 (58.59%), Postives = 189/256 (73.83%), Query Frame = 0

Query: 9   GFRMGFRIPDLNQIYIPKRRR--SSGAGNKEEPKIETDFPMSMSEAADILVEISRSVFYV 68
           G R+  ++PDLN IYIP++RR  SSGAG KE    ET+ P+SM+EAA IL++++ SV  V
Sbjct: 10  GLRICLQLPDLNDIYIPRKRRGSSSGAGKKERSMTETELPLSMAEAASILIQLANSVPDV 69

Query: 69  EPEKINKTHKRKKPSQPT--PTTMTKKQMN------------KKAKKS--KSLSILEMPA 128
           + +K+NKTHKRK P   T   T+ TKKQ N            KK +K   K +    MP 
Sbjct: 70  QRQKLNKTHKRKNPPTLTEASTSRTKKQRNPPTLTEASTSRTKKQRKKYVKQIEHPSMPV 129

Query: 129 AMRDRIVKMGGYEINLVIQKQLQDTDLNKNQGRLSLPTEKLLFDFATEEEKKLLSQRENK 188
           AMRDRI++MGGYEI LVIQKQL DTDLNKN GRLS+ T++L FDFATEEE KLLS++ENK
Sbjct: 130 AMRDRILEMGGYEIRLVIQKQLTDTDLNKNHGRLSMNTKQLSFDFATEEETKLLSEQENK 189

Query: 189 KKKGMNVLIMDNVSEERRICLKKWKIGSGDVYCLMTQWNAFAEETGLKSGQHIQIWCFRR 247
            K+G+NV+++D+V EER +CLKKWKIGSG+VYCLMT+WN   EE G K G+ IQ+W FR+
Sbjct: 190 NKRGINVMMLDDVLEERMLCLKKWKIGSGEVYCLMTRWNLMVEERGFKGGEEIQVWSFRK 249

BLAST of Bhi12G000831 vs. ExPASy TrEMBL
Match: A0A5A7TS79 (Putative B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold149G001480 PE=4 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 1.3e-73
Identity = 146/242 (60.33%), Postives = 187/242 (77.27%), Query Frame = 0

Query: 9   GFRMGFRIPDLNQIYIPKRRR--SSGAGNKEEPKIETDFPMSMSEAADILVEISRSVFYV 68
           G R+   +PDL+ IYIP++RR  SSGAG KE+   ET+ P+SM+EAA IL++++ SV  V
Sbjct: 10  GLRICHHLPDLDNIYIPRKRRGSSSGAGKKEQSITETELPLSMAEAASILIQLANSVPDV 69

Query: 69  EPEKINKTHKRKKPSQPT--PTTMTKKQMNKKAKKSKSLSILEMPAAMRDRIVKMGGYEI 128
           + +K+NKTHKRK P  PT   T+ TKKQ  K   +S+   I  MP  MRDRI++MGGYEI
Sbjct: 70  QRQKLNKTHKRKNPPIPTEASTSKTKKQRKKNVDQSE---IPSMPVTMRDRILEMGGYEI 129

Query: 129 NLVIQKQLQDTDLNKNQGRLSLPTEKLLFDFATEEEKKLLSQRENKKKKGMNVLIMDNVS 188
            LVIQKQL DTDLNKN GRLS+  ++L FDFATEEE KLLS++ENK KKG+NV+I+D+V 
Sbjct: 130 RLVIQKQLTDTDLNKNHGRLSMNKKQLSFDFATEEEGKLLSEQENKNKKGINVMILDDVL 189

Query: 189 EERRICLKKWKIGSGDVYCLMTQWNAFAEETGLKSGQHIQIWCFRRDDEFEASRLCFGMV 247
           EER +CLKKWKIG+G+VYCLMT+WN   E+ G +SG+ IQ+W FR+DDE+EA RLCF +V
Sbjct: 190 EERMLCLKKWKIGNGEVYCLMTKWNLMVEKRGFESGEEIQVWSFRKDDEYEAHRLCFALV 248

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT2G31420.18.3e-1235.66Domain of unknown function (DUF313) [more]
AT3G24850.17.0e-1130.69Domain of unknown function (DUF313) [more]
AT1G05920.12.7e-1030.83Domain of unknown function (DUF313) [more]
AT2G31720.12.7e-1028.46Domain of unknown function (DUF313) [more]
AT2G24670.14.7e-0724.70Domain of unknown function (DUF313) [more]
Match NameE-valueIdentityDescription
Q9SIC71.2e-1035.66B3 domain-containing protein At2g31420 OS=Arabidopsis thaliana OX=3702 GN=At2g31... [more]
Q9LRX69.9e-1030.69Putative B3 domain-containing protein At3g24850 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9LSF72.9e-0930.73B3 domain-containing protein At3g25182 OS=Arabidopsis thaliana OX=3702 GN=At3g25... [more]
Q5RM093.7e-0930.83B3 domain-containing protein At1g05920 OS=Arabidopsis thaliana OX=3702 GN=At1g05... [more]
Q8RV833.7e-0928.46B3 domain-containing protein At2g31720 OS=Arabidopsis thaliana OX=3702 GN=ARF70 ... [more]
Match NameE-valueIdentityDescription
XP_038874962.11.2e-134100.00putative B3 domain-containing protein At5g35780 [Benincasa hispida][more]
XP_038874960.11.7e-11289.92B3 domain-containing protein At2g31420-like [Benincasa hispida][more]
XP_038876237.19.9e-10078.93putative B3 domain-containing protein At3g24850 [Benincasa hispida][more]
XP_038907053.14.9e-8365.85B3 domain-containing protein At2g31420-like [Benincasa hispida][more]
XP_008467039.14.3e-7965.16PREDICTED: putative B3 domain-containing protein At3g24850 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A1S3CSS42.1e-7965.16putative B3 domain-containing protein At3g24850 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A5A7TY941.0e-7358.59Putative B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A5A7TWX21.0e-7360.33Putative B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S4E6001.0e-7358.59putative B3 domain-containing protein At3g24850 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A5A7TS791.3e-7360.33Putative B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005508B3 domain-containing protein At2g31720-likePFAMPF03754DUF313coord: 107..210
e-value: 2.8E-18
score: 66.2
IPR005508B3 domain-containing protein At2g31720-likePANTHERPTHR31541B3 DOMAIN PLANT PROTEIN-RELATEDcoord: 18..246
IPR015300DNA-binding pseudobarrel domain superfamilyGENE3D2.40.330.10coord: 119..239
e-value: 2.7E-14
score: 54.9
IPR015300DNA-binding pseudobarrel domain superfamilySUPERFAMILY101936DNA-binding pseudobarrel domaincoord: 125..241
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 71..102
NoneNo IPR availablePANTHERPTHR31541:SF25DOMAIN PROTEIN, PUTATIVE (DUF313)-RELATEDcoord: 18..246

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi12M000831Bhi12M000831mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding