Bhi12G000764 (gene) Wax gourd (B227) v1

Overview
NameBhi12G000764
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationchr12: 26656281 .. 26660647 (-)
RNA-Seq ExpressionBhi12G000764
SyntenyBhi12G000764
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCAGGGAGGTCCATTGCTGGCGCCGGAGATGTCATGAAGAAAGACTGCACCGATCTCATTCGTCGGATTGCTCTCCTGATTCATCTGGCGGAGGAGATCACAAACTTTTGTAGTGGCAGCGGGGAGAATTTTGAGGCATTGAATGATGATGGGTCTTCTTCATCATCTTCTTCGTTGGATTGCCTTTCGGAAGTGGTCGGAGCAATTCAGGCAGCAAAGCGGCTTCTGTACATGGCTTTGACTTTCTCTGTATACGACGAGGAAGGTTGTGGTGCGACTTCGACGGTGAGTTTGATCATTATCGTTGTCTTTCTGTTGTCATTAGATTACATTGTTTGTGTGTTTGAATTGGAAAACGAAAGTCAACAGAGCTGGAAGTGGAACTTCCATAGTTCGATTGATGTAATAACTCAGCACTTCTGAGTTTTTGATTCAGTGCAGTTTTGGCAAATTTTTTTGGCTGCTAAAGCGTTTCTGTTTGCTTTGCTTTGATTTTGCGAGAAATTTCATTCCTACGTGTTGCAGCTGTTTTTGTGGTGGTCGTCGTTTTTTTTTTTTTTTTTTTTGAAAATTTAAAAAGATATATATTTAGGCACGATCCTCATATTTAAGCATTCCATATAATTTTTCAATTCTTTTTTAGTGGGTTGTCCAAAGATTTGTTTATAACAAGAGAATTCAACTTTCAGGCTTCCATTCCATTGATAAAGTTATATAGTTTTTCTTCTTTTACCTTTATATTTGAAAATATTTTATTTTAGTTTTAAAATTTAAAACTATTTAGATCATGTTTGAGGAATCATTTTTAAAAGTGATTTTAATGGAAATAGTTTGTTTCCAAGTGATTTACTAAAAGAGATTTTCATAGGATGTTTGGTTTTATACTTTTAATTTGTGATTATCATATTATTCAAATTGATTTTAAATTATTCAAAACATATTGGAGAGTAATTACGAATACTACATAAGCGATTTAATGATTTCAAAATTACCTTCAAATATGTCCTTATGGTTGATAATTTGTTTTATAAGATACAAAATATTTCTTAGTCTATCTATTTTTGAACTTAACACCGAGGTGTATGTGAATTGGAACCTAAAGCTTATTTGATAACTATTGTTTTTTGTTTTTTCGTTTTGGAAAATCTCCCGCTTTTGAGTTTAATTGTCTTAGCTATTTACTTTCTAAAATATATTTTTAAAAAACCTAAATTCGAAAATCTAAAAAGGGTATTATTTTTGAAATTTGATTAAAAATTCAAATCTTAACTTAAAAGAAAAAAGACTAAAATATAAAGAAACGGCAAGAAAATTAGTATGAGAAACAGAGAGCTAAAGAATGAAATTGTTATTGAATAAGTCTCAATTTTTATGAATTTTTTGTTTGTTAAATACAAAATGGAAATTTAACTGTCAATTACATGTGGTCAATGGTTTATTTTATTTTTGATACAAAGTGGCGAGTTCTAGAGCCAATTGAGAGAAGGAAGAGAAATGTCAACAATAATAAATATTCTTTTTCTTAGCTAATTTAACAAAAAAAAAAACATTGTGGAAAGTTCAATTAGCTTACATTAGCAACCACATCATCATTTTGTTAAGCTAACAAATCATTAACTTGAGAAACAACTAGAAACAGATTTCAAAACCAACTATAATTGAAATTCAAACTAGACCAAACATGTCACTTTTGGATTCTCCAATATCAAATAAAAATATCTTAAGGATTAAATTCTTAAAAACTAAGTTCAAAATTTTGGACCAAAAGTATAAGATACATCAATGAATATATATTTTCATGATTACTGCAGAAAAGTTTAAATAAAATTAAAAGAAAATCTCGATTACGATTACGATTCTGAATATTCAAAATTGTATAGAAGGTTTCAATTACATCTAGAGCTTTTAATTTTATGTTTGATAGACTTTTGCCATTAAATTCGTCAATTCGTTAGTTAATTATTAATTGGGAATTTGTGGTGAAATATGACAACCGAAGAGGATAATATATATATATATAAAATGAAAAAATGGATTATTATATTTTCTTTTAATTCCACTCTGAAGTTTTGCTAATGTTCTTAATAATCTGCTTTGTTCGAATTAAGGAGGAAGCTACCAAAAAATTGGTTCTACAATTTCAATACGTGACAACAAGATTGGAAACTGCACTTTCAAATCTGCCGTACGACCACTTTTGTGTCTCAGACGAAGTACAAGAACAGGTAAACATAACATCCTCATTCCTATCATTAAAGGAAACCGAAGTCTCTTCGAGAAGTTTAGATGTTTGATAGCATGAGATTTCATCTCAAAACTAATTGATAATAAGAGAAGTAACCCATCTATCTTATAAGAAGTATGGGAAATTTTCTAACATTAGATATATTAGCAAAAGAAAAGATCTATGGTATATAAGTGAGGATAATTTGAATAGTCTCTTCCTCGAATAATGTTTGAGAAAGTTATTGTTTTGTTTGTTAAGGTTGATTTGGTTAGAGCTCAATTGAGGAGAGCATCAAACAAGTATGAATCTATGTCTAACCCTGCAGAAAAGAAGCTTCAAACTCGAGGTTCTGTAAAATGGATGATCAATAATGATGTCAAGAGTATTTCTAGTGTTGATGATGGAGATGTAGAGTCTCAGCATCATCCTCGGAACTGCAATTATCCGACTAGTTTCGATTCGGTGAATTCTTGTTTTGATGAGAGTTCAAGTGTTGTTCATTCTGATATGGAAGATGTTCTAGCCAGCAAGAGCCAAGATGAGGTTAGGAAGCCTATTGAAGTTGACATTCCTGAAAACTTCTTGTGCCCCATTTCCTTTGAGTTGATGTTGGATCCCGTCATCTGCTCGACCGGACAGGTACAATCACCATTGATTGAATCTTTGTTTAAGTATTGATATAATTAAATGTATCGTAACCCATCCATTTAAGTTTTTAAGTTGATTGGTGATTTAACTGTTCTATTTTTTTGTTTGGCTTTTATGAAAGCTTTTAACCAAACATGTTTTCACTTAATTTAGTTCGAAAAAGAAAAAAAGTTAAAATGAAACCGTTTTGGAATGGGGCTCGGACGGATTTTGTTGGAATGAGATTCGTATTTATATTGTTTACTATTTTATCCTTAGGATTTAGAGGAGTGTGCTATGTAAACTTTATAGTTATATTGGTGCCTTTGAATCTATATTCTTATGTTTTTCTGTAACACACTAAATCTCTGTTTCGATTTCTCTTCTTTCTACGTTTTTTGTTTTCTTTGTTTGTCAATTTCATAACCGTCTTAATTATGATCCTTTGTAGACATATGAAAGATCCAACGTACAAAATTGGATAGACAGAGGAAATAGAACATGTCCAAAAACTCAGGAGCAGCTCCAAACTCTGATCCTCACTCCAAATTTTCTAATGAGAAAACTGATAGCTGAATGGTGTGAAGAGCACAATGTGAAGCTAGAGGAAGGATTAACCAGCAGGAAATTTAAGAAGTATAGATCCTTCGAAGACGACTGCCGAAGGACTCTGCCGATAAAAACTCTCGTTCGACACCTTTCATTCGGGTCAATTCAGGAGCAGAAAATAGCTGTAACCGAGATTCGAAAACTATCGAAAAGCAGCTCAGATCATAGAGTTGAAATAGCAGAAGCAGGAGCAATCCCACAGCTGGTTAACCTTTTAACTTCAGAAGATATTGTAACACAAGAGAATGCAATGTCTTGCATTCTCAACCTTTCACTTCATGAGCAAAACAAGAGACTTGTTATGCTTTCTGGTGCTGTTTCTTACATTTCCAAAGTCCTCAAAGTTGGGAGCATGGAAGGGAGAGAGTGTGCTGCTGCGACGATTTACAGCTTGTCGTTAGCCGATGAGAACAAGGCAATAATCGGGGCTTCAGGTGTGATACCGGACCTGTTAGAAATTCTCGAGATCGGTAGCCCGAGAGGGCAGAAAGATGCTGCAGGAGCTCTATTGAATTTGTGTATGTACCAAGGGAACAAGGGCAGGGCTTTGAAGGCTGGGATTGTCAGACCATTGTTGAAAATACTCTCTGATCCAAATGGATCTTTGGTTGATGATGCTCTCTATATAATGTCAGTTCTTTGCGGCCACCCAGAAGCGAAAGCTGCAATGGCGAATGCGAATACGCTGCTCGTATTGACCGATGTTCTGAAGATGGGATCCTCTCGCAGCAAGGAAAATGCAGCTGCAGTTCTGCTTGCATTGTGCAAGGGAGATTGGGAGAAGCTGGAATGGTTAACTAGGCTTGGCGCAATGGCGTCGTTGATGAAACTTGCGGAGAACGGCACGGGGAGAGCGAGGCGGAAGGCTGCTTCATTGTTGGAACAACTCAGAAAGTCATGA

mRNA sequence

ATGTCAGGGAGGTCCATTGCTGGCGCCGGAGATGTCATGAAGAAAGACTGCACCGATCTCATTCGTCGGATTGCTCTCCTGATTCATCTGGCGGAGGAGATCACAAACTTTTGTAGTGGCAGCGGGGAGAATTTTGAGGCATTGAATGATGATGGGTCTTCTTCATCATCTTCTTCGTTGGATTGCCTTTCGGAAGTGGTCGGAGCAATTCAGGCAGCAAAGCGGCTTCTGTACATGGCTTTGACTTTCTCTGTATACGACGAGGAAGGTTGTGGTGCGACTTCGACGGAGGAAGCTACCAAAAAATTGGTTCTACAATTTCAATACGTGACAACAAGATTGGAAACTGCACTTTCAAATCTGCCGTACGACCACTTTTGTGTCTCAGACGAAGTACAAGAACAGGTTGATTTGGTTAGAGCTCAATTGAGGAGAGCATCAAACAAGTATGAATCTATGTCTAACCCTGCAGAAAAGAAGCTTCAAACTCGAGGTTCTGTAAAATGGATGATCAATAATGATGTCAAGAGTATTTCTAGTGTTGATGATGGAGATGTAGAGTCTCAGCATCATCCTCGGAACTGCAATTATCCGACTAGTTTCGATTCGGTGAATTCTTGTTTTGATGAGAGTTCAAGTGTTGTTCATTCTGATATGGAAGATGTTCTAGCCAGCAAGAGCCAAGATGAGGTTAGGAAGCCTATTGAAGTTGACATTCCTGAAAACTTCTTGTGCCCCATTTCCTTTGAGTTGATGTTGGATCCCGTCATCTGCTCGACCGGACAGACATATGAAAGATCCAACGTACAAAATTGGATAGACAGAGGAAATAGAACATGTCCAAAAACTCAGGAGCAGCTCCAAACTCTGATCCTCACTCCAAATTTTCTAATGAGAAAACTGATAGCTGAATGGTGTGAAGAGCACAATGTGAAGCTAGAGGAAGGATTAACCAGCAGGAAATTTAAGAAGTATAGATCCTTCGAAGACGACTGCCGAAGGACTCTGCCGATAAAAACTCTCGTTCGACACCTTTCATTCGGGTCAATTCAGGAGCAGAAAATAGCTGTAACCGAGATTCGAAAACTATCGAAAAGCAGCTCAGATCATAGAGTTGAAATAGCAGAAGCAGGAGCAATCCCACAGCTGGTTAACCTTTTAACTTCAGAAGATATTGTAACACAAGAGAATGCAATGTCTTGCATTCTCAACCTTTCACTTCATGAGCAAAACAAGAGACTTGTTATGCTTTCTGGTGCTGTTTCTTACATTTCCAAAGTCCTCAAAGTTGGGAGCATGGAAGGGAGAGAGTGTGCTGCTGCGACGATTTACAGCTTGTCGTTAGCCGATGAGAACAAGGCAATAATCGGGGCTTCAGGTGTGATACCGGACCTGTTAGAAATTCTCGAGATCGGTAGCCCGAGAGGGCAGAAAGATGCTGCAGGAGCTCTATTGAATTTGTGTATGTACCAAGGGAACAAGGGCAGGGCTTTGAAGGCTGGGATTGTCAGACCATTGTTGAAAATACTCTCTGATCCAAATGGATCTTTGGTTGATGATGCTCTCTATATAATGTCAGTTCTTTGCGGCCACCCAGAAGCGAAAGCTGCAATGGCGAATGCGAATACGCTGCTCGTATTGACCGATGTTCTGAAGATGGGATCCTCTCGCAGCAAGGAAAATGCAGCTGCAGTTCTGCTTGCATTGTGCAAGGGAGATTGGGAGAAGCTGGAATGGTTAACTAGGCTTGGCGCAATGGCGTCGTTGATGAAACTTGCGGAGAACGGCACGGGGAGAGCGAGGCGGAAGGCTGCTTCATTGTTGGAACAACTCAGAAAGTCATGA

Coding sequence (CDS)

ATGTCAGGGAGGTCCATTGCTGGCGCCGGAGATGTCATGAAGAAAGACTGCACCGATCTCATTCGTCGGATTGCTCTCCTGATTCATCTGGCGGAGGAGATCACAAACTTTTGTAGTGGCAGCGGGGAGAATTTTGAGGCATTGAATGATGATGGGTCTTCTTCATCATCTTCTTCGTTGGATTGCCTTTCGGAAGTGGTCGGAGCAATTCAGGCAGCAAAGCGGCTTCTGTACATGGCTTTGACTTTCTCTGTATACGACGAGGAAGGTTGTGGTGCGACTTCGACGGAGGAAGCTACCAAAAAATTGGTTCTACAATTTCAATACGTGACAACAAGATTGGAAACTGCACTTTCAAATCTGCCGTACGACCACTTTTGTGTCTCAGACGAAGTACAAGAACAGGTTGATTTGGTTAGAGCTCAATTGAGGAGAGCATCAAACAAGTATGAATCTATGTCTAACCCTGCAGAAAAGAAGCTTCAAACTCGAGGTTCTGTAAAATGGATGATCAATAATGATGTCAAGAGTATTTCTAGTGTTGATGATGGAGATGTAGAGTCTCAGCATCATCCTCGGAACTGCAATTATCCGACTAGTTTCGATTCGGTGAATTCTTGTTTTGATGAGAGTTCAAGTGTTGTTCATTCTGATATGGAAGATGTTCTAGCCAGCAAGAGCCAAGATGAGGTTAGGAAGCCTATTGAAGTTGACATTCCTGAAAACTTCTTGTGCCCCATTTCCTTTGAGTTGATGTTGGATCCCGTCATCTGCTCGACCGGACAGACATATGAAAGATCCAACGTACAAAATTGGATAGACAGAGGAAATAGAACATGTCCAAAAACTCAGGAGCAGCTCCAAACTCTGATCCTCACTCCAAATTTTCTAATGAGAAAACTGATAGCTGAATGGTGTGAAGAGCACAATGTGAAGCTAGAGGAAGGATTAACCAGCAGGAAATTTAAGAAGTATAGATCCTTCGAAGACGACTGCCGAAGGACTCTGCCGATAAAAACTCTCGTTCGACACCTTTCATTCGGGTCAATTCAGGAGCAGAAAATAGCTGTAACCGAGATTCGAAAACTATCGAAAAGCAGCTCAGATCATAGAGTTGAAATAGCAGAAGCAGGAGCAATCCCACAGCTGGTTAACCTTTTAACTTCAGAAGATATTGTAACACAAGAGAATGCAATGTCTTGCATTCTCAACCTTTCACTTCATGAGCAAAACAAGAGACTTGTTATGCTTTCTGGTGCTGTTTCTTACATTTCCAAAGTCCTCAAAGTTGGGAGCATGGAAGGGAGAGAGTGTGCTGCTGCGACGATTTACAGCTTGTCGTTAGCCGATGAGAACAAGGCAATAATCGGGGCTTCAGGTGTGATACCGGACCTGTTAGAAATTCTCGAGATCGGTAGCCCGAGAGGGCAGAAAGATGCTGCAGGAGCTCTATTGAATTTGTGTATGTACCAAGGGAACAAGGGCAGGGCTTTGAAGGCTGGGATTGTCAGACCATTGTTGAAAATACTCTCTGATCCAAATGGATCTTTGGTTGATGATGCTCTCTATATAATGTCAGTTCTTTGCGGCCACCCAGAAGCGAAAGCTGCAATGGCGAATGCGAATACGCTGCTCGTATTGACCGATGTTCTGAAGATGGGATCCTCTCGCAGCAAGGAAAATGCAGCTGCAGTTCTGCTTGCATTGTGCAAGGGAGATTGGGAGAAGCTGGAATGGTTAACTAGGCTTGGCGCAATGGCGTCGTTGATGAAACTTGCGGAGAACGGCACGGGGAGAGCGAGGCGGAAGGCTGCTTCATTGTTGGAACAACTCAGAAAGTCATGA

Protein sequence

MSGRSIAGAGDVMKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSSLDCLSEVVGAIQAAKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSNLPYDHFCVSDEVQEQVDLVRAQLRRASNKYESMSNPAEKKLQTRGSVKWMINNDVKSISSVDDGDVESQHHPRNCNYPTSFDSVNSCFDESSSVVHSDMEDVLASKSQDEVRKPIEVDIPENFLCPISFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPNFLMRKLIAEWCEEHNVKLEEGLTSRKFKKYRSFEDDCRRTLPIKTLVRHLSFGSIQEQKIAVTEIRKLSKSSSDHRVEIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLHEQNKRLVMLSGAVSYISKVLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSPRGQKDAAGALLNLCMYQGNKGRALKAGIVRPLLKILSDPNGSLVDDALYIMSVLCGHPEAKAAMANANTLLVLTDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLAENGTGRARRKAASLLEQLRKS
Homology
BLAST of Bhi12G000764 vs. TAIR 10
Match: AT1G23030.1 (ARM repeat superfamily protein )

HSP 1 Score: 498.8 bits (1283), Expect = 6.2e-141
Identity = 294/609 (48.28%), Postives = 402/609 (66.01%), Query Frame = 0

Query: 12  VMKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSSLDCLSEVVGAIQ 71
           + KKDC DL RR+ LL HL EEI           ++   D ++SSSS  D  S++V  +Q
Sbjct: 27  MFKKDCADLTRRVCLLTHLLEEIR----------DSTPIDSAASSSSENDWWSDLVVGLQ 86

Query: 72  AAKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSNLPYDHFCVSDE 131
           AAKRLL  A  F   D       S++ A K++  QFQ VT +LE ALSNLPYD + +SDE
Sbjct: 87  AAKRLLSTA-RFQARD-------SSDGAAKRISFQFQCVTWKLEKALSNLPYDLYDISDE 146

Query: 132 VQEQVDLVRAQLRRASNKYESMSNPAEKKLQTRGSVKWMINNDVK-----SISSVDDGDV 191
           V EQV+L R+QLRRA  +Y S+++       +    +   +N +K      + SV +   
Sbjct: 147 VGEQVELARSQLRRAMQRYGSLNSNKFSSALSEPMERDGFSNVIKIKAEEKLESVSETLH 206

Query: 192 ESQHHPRNCNYPTSFDSVNSCFDESSSVVHSDMEDVLASKSQDEVRKPIEVDIPENFLCP 251
             +   +  + P    S  S     S    +D  D + +K+ DE +K  ++ IP +FLCP
Sbjct: 207 FGEEEEKQSSPPLRRSSSISLAYYLSKDADTDRLDKMVNKNTDESKKSDKLTIPVDFLCP 266

Query: 252 ISFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPNFLMRKLIAEWC 311
           +S ELM DPVI +TGQTYER+ +Q WID GN TCPKTQ++L+   LTPN+++R LI+ WC
Sbjct: 267 VSLELMKDPVIVATGQTYERAYIQRWIDCGNLTCPKTQQKLENFTLTPNYVLRSLISRWC 326

Query: 312 EEHNVKLEEGLTSRKFKKYRSFEDDCRRTLPIKTLVRHLSFGSIQEQKIAVTEIRKLSKS 371
            EHN++   G  + + K              I+ LV+ LS  S ++++ AV+EIR LSK 
Sbjct: 327 AEHNIEQPAGYINGRTKNSGDMS-------VIRALVQRLSSRSTEDRRNAVSEIRSLSKR 386

Query: 372 SSDHRVEIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLHEQNKRLVMLSGAVSYISK 431
           S+D+R+ IAEAGAIP LVNLLTSED+ TQENA++C+LNLS++E NK L+M +GAV+ I +
Sbjct: 387 STDNRILIAEAGAIPVLVNLLTSEDVATQENAITCVLNLSIYENNKELIMFAGAVTSIVQ 446

Query: 432 VLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSPRGQKDAAGALLN 491
           VL+ G+ME RE AAAT++SLSLADENK IIG SG IP L+++LE G+PRG+KDAA AL N
Sbjct: 447 VLRAGTMEARENAAATLFSLSLADENKIIIGGSGAIPALVDLLENGTPRGKKDAATALFN 506

Query: 492 LCMYQGNKGRALKAGIVRPLLKILSD-PNGSLVDDALYIMSVLCGHPEAKAAMANANTLL 551
           LC+Y GNKGRA++AGIV  L+K+LSD     +VD+AL I+SVL  + +AK+A+  ANTL 
Sbjct: 507 LCIYHGNKGRAVRAGIVTALVKMLSDSTRHRMVDEALTILSVLANNQDAKSAIVKANTLP 566

Query: 552 VLTDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLAENGTGRARRKAA 611
            L  +L+   +R++ENAAA+LL+LCK D EKL  + RLGA+  LM L++NGT R +RKA 
Sbjct: 567 ALIGILQTDQTRNRENAAAILLSLCKRDTEKLITIGRLGAVVPLMDLSKNGTERGKRKAI 610

Query: 612 SLLEQLRKS 615
           SLLE LRK+
Sbjct: 627 SLLELLRKA 610

BLAST of Bhi12G000764 vs. TAIR 10
Match: AT1G71020.1 (ARM repeat superfamily protein )

HSP 1 Score: 487.3 bits (1253), Expect = 1.9e-137
Identity = 300/621 (48.31%), Postives = 403/621 (64.90%), Query Frame = 0

Query: 6   IAGAGDVMKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSSLDCLSE 65
           I G   + KKDC+DL RR+ LL HL EEI +    S       +D  SS +S   D  S+
Sbjct: 21  IPGNFGLFKKDCSDLARRVGLLTHLIEEIRDSSPPSE------SDASSSLNSHECDWWSD 80

Query: 66  VVGAIQAAKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSNLPYDH 125
           +V  +QAAKRLL  A +F   +       S++ A K++  QFQ VT +LE AL +L YD 
Sbjct: 81  LVVGLQAAKRLLSSATSFQARE-------SSDGAAKRISFQFQCVTWKLEKALGDLTYDR 140

Query: 126 FCVSDEVQEQVDLVRAQLRRASNKYES---------MSNPAEKKLQTRGSVKWMINNDVK 185
           + +SDEV+EQV+L R QLRRA  +Y S         +S P EK   +   V   + +  +
Sbjct: 141 YDISDEVREQVELARLQLRRAMQRYGSLNSKKFSSGLSEPMEKDASSNRKVIEKLESIPE 200

Query: 186 SISSVDDGDVESQHHPRNCNYPTSFDSVNSCFDESSSVVHSDMEDVLASKSQDEVRKPIE 245
           ++ S+ D + + +  P     P    SV+  F  S       +E  +   S D+ +K   
Sbjct: 201 TVHSLSD-EKKFESPP-----PWKSSSVSLAFFLSKDGDDERLEKAVTENS-DDSQKSDN 260

Query: 246 VDIPENFLCPISFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPNF 305
           + IPE+FLCPIS ELM DP I STGQTYERS +Q WID GN +CPKTQ++L+   LTPN+
Sbjct: 261 LTIPEDFLCPISLELMKDPAIVSTGQTYERSFIQRWIDCGNLSCPKTQQKLENFTLTPNY 320

Query: 306 LMRKLIAEWCEEHNVKLEEG-LTSRKFKKYRSFEDDCRRTLPIKTLVRHLSFGSIQEQKI 365
           ++R LI++WC +HN++   G +  R      SF D       I+ LV  LS  SI++++ 
Sbjct: 321 VLRSLISQWCTKHNIEQPGGYMNGRTKNSDGSFRDLSGDMSAIRALVCKLSSQSIEDRRT 380

Query: 366 AVTEIRKLSKSSSDHRVEIAEAGAIPQLVNLLTSE-DIVTQENAMSCILNLSLHEQNKRL 425
           AV+EIR LSK S+D+R+ IAEAGAIP LV LLTS+ D  TQENA++CILNLS++E NK L
Sbjct: 381 AVSEIRSLSKRSTDNRILIAEAGAIPVLVKLLTSDGDTETQENAVTCILNLSIYEHNKEL 440

Query: 426 VMLSGAVSYISKVLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSP 485
           +ML+GAV+ I  VL+ GSME RE AAAT++SLSLADENK IIGASG I  L+++L+ GS 
Sbjct: 441 IMLAGAVTSIVLVLRAGSMEARENAAATLFSLSLADENKIIIGASGAIMALVDLLQYGSV 500

Query: 486 RGQKDAAGALLNLCMYQGNKGRALKAGIVRPLLKILSDPNGS-LVDDALYIMSVLCGHPE 545
           RG+KDAA AL NLC+YQGNKGRA++AGIV+PL+K+L+D +   + D+AL I+SVL  +  
Sbjct: 501 RGKKDAATALFNLCIYQGNKGRAVRAGIVKPLVKMLTDSSSERMADEALTILSVLASNQV 560

Query: 546 AKAAMANANTLLVLTDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLA 605
           AK A+  AN +  L D L+    R++ENAAA+LL LCK D EKL  + RLGA+  LM+L+
Sbjct: 561 AKTAILRANAIPPLIDCLQKDQPRNRENAAAILLCLCKRDTEKLISIGRLGAVVPLMELS 620

Query: 606 ENGTGRARRKAASLLEQLRKS 615
            +GT RA+RKA SLLE LRKS
Sbjct: 621 RDGTERAKRKANSLLELLRKS 621

BLAST of Bhi12G000764 vs. TAIR 10
Match: AT1G71020.2 (ARM repeat superfamily protein )

HSP 1 Score: 399.8 bits (1026), Expect = 3.9e-111
Identity = 238/472 (50.42%), Postives = 320/472 (67.80%), Query Frame = 0

Query: 147 SNKYES-MSNPAEKKLQTRGSVKWMINNDVKSISSVDDGDVESQHHPRNCNYPTSFDSVN 206
           S K+ S +S P EK   +   V   + +  +++ S+ D + + +  P     P    SV+
Sbjct: 9   SKKFSSGLSEPMEKDASSNRKVIEKLESIPETVHSLSD-EKKFESPP-----PWKSSSVS 68

Query: 207 SCFDESSSVVHSDMEDVLASKSQDEVRKPIEVDIPENFLCPISFELMLDPVICSTGQTYE 266
             F  S       +E  +   S D+ +K   + IPE+FLCPIS ELM DP I STGQTYE
Sbjct: 69  LAFFLSKDGDDERLEKAVTENS-DDSQKSDNLTIPEDFLCPISLELMKDPAIVSTGQTYE 128

Query: 267 RSNVQNWIDRGNRTCPKTQEQLQTLILTPNFLMRKLIAEWCEEHNVKLEEG-LTSRKFKK 326
           RS +Q WID GN +CPKTQ++L+   LTPN+++R LI++WC +HN++   G +  R    
Sbjct: 129 RSFIQRWIDCGNLSCPKTQQKLENFTLTPNYVLRSLISQWCTKHNIEQPGGYMNGRTKNS 188

Query: 327 YRSFEDDCRRTLPIKTLVRHLSFGSIQEQKIAVTEIRKLSKSSSDHRVEIAEAGAIPQLV 386
             SF D       I+ LV  LS  SI++++ AV+EIR LSK S+D+R+ IAEAGAIP LV
Sbjct: 189 DGSFRDLSGDMSAIRALVCKLSSQSIEDRRTAVSEIRSLSKRSTDNRILIAEAGAIPVLV 248

Query: 387 NLLTSE-DIVTQENAMSCILNLSLHEQNKRLVMLSGAVSYISKVLKVGSMEGRECAAATI 446
            LLTS+ D  TQENA++CILNLS++E NK L+ML+GAV+ I  VL+ GSME RE AAAT+
Sbjct: 249 KLLTSDGDTETQENAVTCILNLSIYEHNKELIMLAGAVTSIVLVLRAGSMEARENAAATL 308

Query: 447 YSLSLADENKAIIGASGVIPDLLEILEIGSPRGQKDAAGALLNLCMYQGNKGRALKAGIV 506
           +SLSLADENK IIGASG I  L+++L+ GS RG+KDAA AL NLC+YQGNKGRA++AGIV
Sbjct: 309 FSLSLADENKIIIGASGAIMALVDLLQYGSVRGKKDAATALFNLCIYQGNKGRAVRAGIV 368

Query: 507 RPLLKILSDPNGS-LVDDALYIMSVLCGHPEAKAAMANANTLLVLTDVLKMGSSRSKENA 566
           +PL+K+L+D +   + D+AL I+SVL  +  AK A+  AN +  L D L+    R++ENA
Sbjct: 369 KPLVKMLTDSSSERMADEALTILSVLASNQVAKTAILRANAIPPLIDCLQKDQPRNRENA 428

Query: 567 AAVLLALCKGDWEKLEWLTRLGAMASLMKLAENGTGRARRKAASLLEQLRKS 615
           AA+LL LCK D EKL  + RLGA+  LM+L+ +GT RA+RKA SLLE LRKS
Sbjct: 429 AAILLCLCKRDTEKLISIGRLGAVVPLMELSRDGTERAKRKANSLLELLRKS 473

BLAST of Bhi12G000764 vs. TAIR 10
Match: AT3G54850.1 (plant U-box 14 )

HSP 1 Score: 392.5 bits (1007), Expect = 6.2e-109
Identity = 250/617 (40.52%), Postives = 377/617 (61.10%), Query Frame = 0

Query: 19  DLIRRIALLIHLAEEITNFCSGSGENFEALNDD--GSSSSSSSLDCLSEVVGAIQAAKRL 78
           DL+RRI LL    EE+ +       N E   D   G  +   +LD   E+  ++    +L
Sbjct: 39  DLVRRITLLSPFFEELIDV------NVELKKDQITGFEAMRIALDSSLELFRSVNGGSKL 98

Query: 79  LYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSNLPYDHFCVSDEVQEQV 138
                 F ++D +             LV +F+ +T  +E ALS +PY+   VS+EV+EQV
Sbjct: 99  ------FQLFDRD------------SLVEKFRDMTVEIEAALSQIPYEKIEVSEEVREQV 158

Query: 139 DLVRAQLRRASNKYESMS-------NPAEKKLQTRGSVKWMINNDVKSISSVDDGDVESQ 198
            L+  Q +RA  ++E            AE  +     +   ++ +++ ++++D  +++ +
Sbjct: 159 QLLHFQFKRAKERWEESDLQLSHDLAMAENVMDPDPIILKRLSQELQ-LTTID--ELKKE 218

Query: 199 HHPRNCNYPTSFD-SVNSCFDESSSVV----------HSDMEDVLASKSQDEVRKPIEVD 258
            H  +  Y  S+D   + CF+  SS++           SD +    S+     R P+   
Sbjct: 219 SHAIH-EYFLSYDGDPDDCFERMSSLLKNLVDFVTMESSDPDPSTGSRIVSRHRSPV--- 278

Query: 259 IPENFLCPISFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPNFLM 318
           IPE F CPIS ELM DPVI STGQTYERS++Q W+D G++TCPK+QE L    LTPN+++
Sbjct: 279 IPEYFRCPISLELMKDPVIVSTGQTYERSSIQKWLDAGHKTCPKSQETLLHAGLTPNYVL 338

Query: 319 RKLIAEWCEEHNVKLEEGLTS-RKFKKYRSFEDDCRRTLPIKTLVRHLSFGSIQEQKIAV 378
           + LIA WCE + ++L +   S R  K   S   DC RT  + +L+  L+ G+ ++Q+ A 
Sbjct: 339 KSLIALWCESNGIELPQNQGSCRTTKIGGSSSSDCDRTF-VLSLLEKLANGTTEQQRAAA 398

Query: 379 TEIRKLSKSSSDHRVEIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLHEQNKRLVML 438
            E+R L+K + D+RV IAEAGAIP LV LL+S D  TQE++++ +LNLS++E NK  ++ 
Sbjct: 399 GELRLLAKRNVDNRVCIAEAGAIPLLVELLSSPDPRTQEHSVTALLNLSINEGNKGAIVD 458

Query: 439 SGAVSYISKVLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSPRGQ 498
           +GA++ I +VLK GSME RE AAAT++SLS+ DENK  IGA+G I  L+ +LE G+ RG+
Sbjct: 459 AGAITDIVEVLKNGSMEARENAAATLFSLSVIDENKVAIGAAGAIQALISLLEEGTRRGK 518

Query: 499 KDAAGALLNLCMYQGNKGRALKAGIVRPLLKILSDPNGSLVDDALYIMSVLCGHPEAKAA 558
           KDAA A+ NLC+YQGNK RA+K GIV PL ++L D  G +VD+AL I+++L  + E K A
Sbjct: 519 KDAATAIFNLCIYQGNKSRAVKGGIVDPLTRLLKDAGGGMVDEALAILAILSTNQEGKTA 578

Query: 559 MANANTLLVLTDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLAENGT 615
           +A A ++ VL ++++ GS R++ENAAA+L  LC G+ E+L     +GA  +L +L ENGT
Sbjct: 579 IAEAESIPVLVEIIRTGSPRNRENAAAILWYLCIGNIERLNVAREVGADVALKELTENGT 623

BLAST of Bhi12G000764 vs. TAIR 10
Match: AT3G46510.1 (plant U-box 13 )

HSP 1 Score: 379.0 bits (972), Expect = 7.1e-105
Identity = 235/625 (37.60%), Postives = 376/625 (60.16%), Query Frame = 0

Query: 13  MKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSSLDCLSEVVGAIQA 72
           +KK C +L RR+ LL+ + EEI        E+ E +++D       +L  L  +  A+ +
Sbjct: 29  VKKLCYNLARRLKLLVPMFEEIR-------ESNEPISED-------TLKTLMNLKEAMCS 88

Query: 73  AKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSNLPYDHFCVSDEV 132
           AK      L F     +       E+ T KL+     V+ +LE +LS +PY+   +SDEV
Sbjct: 89  AKDY----LKFCSQGSKIYLVMEREQVTSKLM----EVSVKLEQSLSQIPYEELDISDEV 148

Query: 133 QEQVDLVRAQLRRASNKYESMSNPAEKKLQTR-------GSVKWMINNDVKSISSVDDGD 192
           +EQV+LV +Q RRA  + +   +   + LQ+         + + ++    K +  ++  D
Sbjct: 149 REQVELVLSQFRRAKGRVDVSDDELYEDLQSLCNKSSDVDAYQPVLERVAKKLHLMEIPD 208

Query: 193 VESQHHPRNCNYPTSFDSVNSCFDESSSVVHSDMEDVLASKSQDEVRKPIEVD------- 252
           +  +    +    +S   V    +E + V+   ++D + ++  +   + + V+       
Sbjct: 209 LAQESVALHEMVASSGGDVGENIEEMAMVLKM-IKDFVQTEDDNGEEQKVGVNSRSNGQT 268

Query: 253 ----------IPENFLCPISFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQ 312
                     IP++F CPIS E+M DPVI S+GQTYER+ ++ WI+ G+ TCPKTQ+ L 
Sbjct: 269 STAASQKIPVIPDDFRCPISLEMMRDPVIVSSGQTYERTCIEKWIEGGHSTCPKTQQALT 328

Query: 313 TLILTPNFLMRKLIAEWCEEHNVKLEEGLTSRKFKKYRSFEDDCRRTLPIKTLVRHLSFG 372
           +  LTPN+++R LIA+WCE ++++  +  +S + +K  SF         I+ L+  L++G
Sbjct: 329 STTLTPNYVLRSLIAQWCEANDIEPPKPPSSLRPRKVSSFSSPAEAN-KIEDLMWRLAYG 388

Query: 373 SIQEQKIAVTEIRKLSKSSSDHRVEIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLH 432
           + ++Q+ A  EIR L+K ++D+RV IAEAGAIP LV LL++ D   QE++++ +LNLS+ 
Sbjct: 389 NPEDQRSAAGEIRLLAKRNADNRVAIAEAGAIPLLVGLLSTPDSRIQEHSVTALLNLSIC 448

Query: 433 EQNKRLVMLSGAVSYISKVLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEI 492
           E NK  ++ +GA+  I +VLK GSME RE AAAT++SLS+ DENK  IGA G IP L+ +
Sbjct: 449 ENNKGAIVSAGAIPGIVQVLKKGSMEARENAAATLFSLSVIDENKVTIGALGAIPPLVVL 508

Query: 493 LEIGSPRGQKDAAGALLNLCMYQGNKGRALKAGIVRPLLKILSDPNGSLVDDALYIMSVL 552
           L  G+ RG+KDAA AL NLC+YQGNKG+A++AG++  L ++L++P   +VD+AL I+++L
Sbjct: 509 LNEGTQRGKKDAATALFNLCIYQGNKGKAIRAGVIPTLTRLLTEPGSGMVDEALAILAIL 568

Query: 553 CGHPEAKAAMANANTLLVLTDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMAS 612
             HPE KA + +++ +  L + ++ GS R++ENAAAVL+ LC GD + L    +LG M  
Sbjct: 569 SSHPEGKAIIGSSDAVPSLVEFIRTGSPRNRENAAAVLVHLCSGDPQHLVEAQKLGLMGP 628

Query: 613 LMKLAENGTGRARRKAASLLEQLRK 614
           L+ LA NGT R +RKAA LLE++ +
Sbjct: 629 LIDLAGNGTDRGKRKAAQLLERISR 629

BLAST of Bhi12G000764 vs. ExPASy Swiss-Prot
Match: Q8GUG9 (U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2 SV=2)

HSP 1 Score: 498.8 bits (1283), Expect = 8.7e-140
Identity = 294/609 (48.28%), Postives = 402/609 (66.01%), Query Frame = 0

Query: 12  VMKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSSLDCLSEVVGAIQ 71
           + KKDC DL RR+ LL HL EEI           ++   D ++SSSS  D  S++V  +Q
Sbjct: 27  MFKKDCADLTRRVCLLTHLLEEIR----------DSTPIDSAASSSSENDWWSDLVVGLQ 86

Query: 72  AAKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSNLPYDHFCVSDE 131
           AAKRLL  A  F   D       S++ A K++  QFQ VT +LE ALSNLPYD + +SDE
Sbjct: 87  AAKRLLSTA-RFQARD-------SSDGAAKRISFQFQCVTWKLEKALSNLPYDLYDISDE 146

Query: 132 VQEQVDLVRAQLRRASNKYESMSNPAEKKLQTRGSVKWMINNDVK-----SISSVDDGDV 191
           V EQV+L R+QLRRA  +Y S+++       +    +   +N +K      + SV +   
Sbjct: 147 VGEQVELARSQLRRAMQRYGSLNSNKFSSALSEPMERDGFSNVIKIKAEEKLESVSETLH 206

Query: 192 ESQHHPRNCNYPTSFDSVNSCFDESSSVVHSDMEDVLASKSQDEVRKPIEVDIPENFLCP 251
             +   +  + P    S  S     S    +D  D + +K+ DE +K  ++ IP +FLCP
Sbjct: 207 FGEEEEKQSSPPLRRSSSISLAYYLSKDADTDRLDKMVNKNTDESKKSDKLTIPVDFLCP 266

Query: 252 ISFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPNFLMRKLIAEWC 311
           +S ELM DPVI +TGQTYER+ +Q WID GN TCPKTQ++L+   LTPN+++R LI+ WC
Sbjct: 267 VSLELMKDPVIVATGQTYERAYIQRWIDCGNLTCPKTQQKLENFTLTPNYVLRSLISRWC 326

Query: 312 EEHNVKLEEGLTSRKFKKYRSFEDDCRRTLPIKTLVRHLSFGSIQEQKIAVTEIRKLSKS 371
            EHN++   G  + + K              I+ LV+ LS  S ++++ AV+EIR LSK 
Sbjct: 327 AEHNIEQPAGYINGRTKNSGDMS-------VIRALVQRLSSRSTEDRRNAVSEIRSLSKR 386

Query: 372 SSDHRVEIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLHEQNKRLVMLSGAVSYISK 431
           S+D+R+ IAEAGAIP LVNLLTSED+ TQENA++C+LNLS++E NK L+M +GAV+ I +
Sbjct: 387 STDNRILIAEAGAIPVLVNLLTSEDVATQENAITCVLNLSIYENNKELIMFAGAVTSIVQ 446

Query: 432 VLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSPRGQKDAAGALLN 491
           VL+ G+ME RE AAAT++SLSLADENK IIG SG IP L+++LE G+PRG+KDAA AL N
Sbjct: 447 VLRAGTMEARENAAATLFSLSLADENKIIIGGSGAIPALVDLLENGTPRGKKDAATALFN 506

Query: 492 LCMYQGNKGRALKAGIVRPLLKILSD-PNGSLVDDALYIMSVLCGHPEAKAAMANANTLL 551
           LC+Y GNKGRA++AGIV  L+K+LSD     +VD+AL I+SVL  + +AK+A+  ANTL 
Sbjct: 507 LCIYHGNKGRAVRAGIVTALVKMLSDSTRHRMVDEALTILSVLANNQDAKSAIVKANTLP 566

Query: 552 VLTDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLAENGTGRARRKAA 611
            L  +L+   +R++ENAAA+LL+LCK D EKL  + RLGA+  LM L++NGT R +RKA 
Sbjct: 567 ALIGILQTDQTRNRENAAAILLSLCKRDTEKLITIGRLGAVVPLMDLSKNGTERGKRKAI 610

Query: 612 SLLEQLRKS 615
           SLLE LRK+
Sbjct: 627 SLLELLRKA 610

BLAST of Bhi12G000764 vs. ExPASy Swiss-Prot
Match: Q9C9A6 (U-box domain-containing protein 10 OS=Arabidopsis thaliana OX=3702 GN=PUB10 PE=2 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 2.6e-136
Identity = 300/621 (48.31%), Postives = 403/621 (64.90%), Query Frame = 0

Query: 6   IAGAGDVMKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSSLDCLSE 65
           I G   + KKDC+DL RR+ LL HL EEI +    S       +D  SS +S   D  S+
Sbjct: 21  IPGNFGLFKKDCSDLARRVGLLTHLIEEIRDSSPPSE------SDASSSLNSHECDWWSD 80

Query: 66  VVGAIQAAKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSNLPYDH 125
           +V  +QAAKRLL  A +F   +       S++ A K++  QFQ VT +LE AL +L YD 
Sbjct: 81  LVVGLQAAKRLLSSATSFQARE-------SSDGAAKRISFQFQCVTWKLEKALGDLTYDR 140

Query: 126 FCVSDEVQEQVDLVRAQLRRASNKYES---------MSNPAEKKLQTRGSVKWMINNDVK 185
           + +SDEV+EQV+L R QLRRA  +Y S         +S P EK   +   V   + +  +
Sbjct: 141 YDISDEVREQVELARLQLRRAMQRYGSLNSKKFSSGLSEPMEKDASSNRKVIEKLESIPE 200

Query: 186 SISSVDDGDVESQHHPRNCNYPTSFDSVNSCFDESSSVVHSDMEDVLASKSQDEVRKPIE 245
           ++ S+ D + + +  P     P    SV+  F  S       +E  +   S D+ +K   
Sbjct: 201 TVHSLSD-EKKFESPP-----PWKSSSVSLAFFLSKDGDDERLEKAVTENS-DDSQKSDN 260

Query: 246 VDIPENFLCPISFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPNF 305
           + IPE+FLCPIS ELM DP I STGQTYERS +Q WID GN +CPKTQ++L+   LTPN+
Sbjct: 261 LTIPEDFLCPISLELMKDPAIVSTGQTYERSFIQRWIDCGNLSCPKTQQKLENFTLTPNY 320

Query: 306 LMRKLIAEWCEEHNVKLEEG-LTSRKFKKYRSFEDDCRRTLPIKTLVRHLSFGSIQEQKI 365
           ++R LI++WC +HN++   G +  R      SF D       I+ LV  LS  SI++++ 
Sbjct: 321 VLRSLISQWCTKHNIEQPGGYMNGRTKNSDGSFRDLSGDMSAIRALVCKLSSQSIEDRRT 380

Query: 366 AVTEIRKLSKSSSDHRVEIAEAGAIPQLVNLLTSE-DIVTQENAMSCILNLSLHEQNKRL 425
           AV+EIR LSK S+D+R+ IAEAGAIP LV LLTS+ D  TQENA++CILNLS++E NK L
Sbjct: 381 AVSEIRSLSKRSTDNRILIAEAGAIPVLVKLLTSDGDTETQENAVTCILNLSIYEHNKEL 440

Query: 426 VMLSGAVSYISKVLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSP 485
           +ML+GAV+ I  VL+ GSME RE AAAT++SLSLADENK IIGASG I  L+++L+ GS 
Sbjct: 441 IMLAGAVTSIVLVLRAGSMEARENAAATLFSLSLADENKIIIGASGAIMALVDLLQYGSV 500

Query: 486 RGQKDAAGALLNLCMYQGNKGRALKAGIVRPLLKILSDPNGS-LVDDALYIMSVLCGHPE 545
           RG+KDAA AL NLC+YQGNKGRA++AGIV+PL+K+L+D +   + D+AL I+SVL  +  
Sbjct: 501 RGKKDAATALFNLCIYQGNKGRAVRAGIVKPLVKMLTDSSSERMADEALTILSVLASNQV 560

Query: 546 AKAAMANANTLLVLTDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLA 605
           AK A+  AN +  L D L+    R++ENAAA+LL LCK D EKL  + RLGA+  LM+L+
Sbjct: 561 AKTAILRANAIPPLIDCLQKDQPRNRENAAAILLCLCKRDTEKLISIGRLGAVVPLMELS 620

Query: 606 ENGTGRARRKAASLLEQLRKS 615
            +GT RA+RKA SLLE LRKS
Sbjct: 621 RDGTERAKRKANSLLELLRKS 621

BLAST of Bhi12G000764 vs. ExPASy Swiss-Prot
Match: Q8VZ40 (U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 8.7e-108
Identity = 250/617 (40.52%), Postives = 377/617 (61.10%), Query Frame = 0

Query: 19  DLIRRIALLIHLAEEITNFCSGSGENFEALNDD--GSSSSSSSLDCLSEVVGAIQAAKRL 78
           DL+RRI LL    EE+ +       N E   D   G  +   +LD   E+  ++    +L
Sbjct: 39  DLVRRITLLSPFFEELIDV------NVELKKDQITGFEAMRIALDSSLELFRSVNGGSKL 98

Query: 79  LYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSNLPYDHFCVSDEVQEQV 138
                 F ++D +             LV +F+ +T  +E ALS +PY+   VS+EV+EQV
Sbjct: 99  ------FQLFDRD------------SLVEKFRDMTVEIEAALSQIPYEKIEVSEEVREQV 158

Query: 139 DLVRAQLRRASNKYESMS-------NPAEKKLQTRGSVKWMINNDVKSISSVDDGDVESQ 198
            L+  Q +RA  ++E            AE  +     +   ++ +++ ++++D  +++ +
Sbjct: 159 QLLHFQFKRAKERWEESDLQLSHDLAMAENVMDPDPIILKRLSQELQ-LTTID--ELKKE 218

Query: 199 HHPRNCNYPTSFD-SVNSCFDESSSVV----------HSDMEDVLASKSQDEVRKPIEVD 258
            H  +  Y  S+D   + CF+  SS++           SD +    S+     R P+   
Sbjct: 219 SHAIH-EYFLSYDGDPDDCFERMSSLLKNLVDFVTMESSDPDPSTGSRIVSRHRSPV--- 278

Query: 259 IPENFLCPISFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPNFLM 318
           IPE F CPIS ELM DPVI STGQTYERS++Q W+D G++TCPK+QE L    LTPN+++
Sbjct: 279 IPEYFRCPISLELMKDPVIVSTGQTYERSSIQKWLDAGHKTCPKSQETLLHAGLTPNYVL 338

Query: 319 RKLIAEWCEEHNVKLEEGLTS-RKFKKYRSFEDDCRRTLPIKTLVRHLSFGSIQEQKIAV 378
           + LIA WCE + ++L +   S R  K   S   DC RT  + +L+  L+ G+ ++Q+ A 
Sbjct: 339 KSLIALWCESNGIELPQNQGSCRTTKIGGSSSSDCDRTF-VLSLLEKLANGTTEQQRAAA 398

Query: 379 TEIRKLSKSSSDHRVEIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLHEQNKRLVML 438
            E+R L+K + D+RV IAEAGAIP LV LL+S D  TQE++++ +LNLS++E NK  ++ 
Sbjct: 399 GELRLLAKRNVDNRVCIAEAGAIPLLVELLSSPDPRTQEHSVTALLNLSINEGNKGAIVD 458

Query: 439 SGAVSYISKVLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSPRGQ 498
           +GA++ I +VLK GSME RE AAAT++SLS+ DENK  IGA+G I  L+ +LE G+ RG+
Sbjct: 459 AGAITDIVEVLKNGSMEARENAAATLFSLSVIDENKVAIGAAGAIQALISLLEEGTRRGK 518

Query: 499 KDAAGALLNLCMYQGNKGRALKAGIVRPLLKILSDPNGSLVDDALYIMSVLCGHPEAKAA 558
           KDAA A+ NLC+YQGNK RA+K GIV PL ++L D  G +VD+AL I+++L  + E K A
Sbjct: 519 KDAATAIFNLCIYQGNKSRAVKGGIVDPLTRLLKDAGGGMVDEALAILAILSTNQEGKTA 578

Query: 559 MANANTLLVLTDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLAENGT 615
           +A A ++ VL ++++ GS R++ENAAA+L  LC G+ E+L     +GA  +L +L ENGT
Sbjct: 579 IAEAESIPVLVEIIRTGSPRNRENAAAILWYLCIGNIERLNVAREVGADVALKELTENGT 623

BLAST of Bhi12G000764 vs. ExPASy Swiss-Prot
Match: Q5VRH9 (U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 4.8e-106
Identity = 253/621 (40.74%), Postives = 364/621 (58.62%), Query Frame = 0

Query: 13  MKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSSLDCLSEVVGAIQA 72
           +++ C DL RR+ LL  L + +                  +SSSSSS   L++ +G   A
Sbjct: 19  LRRPCADLSRRVRLLAPLLDHLP-----------------ASSSSSSSTPLADALG---A 78

Query: 73  AKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSNLPYDHFCVSDEV 132
           A+ LL      S  D+   G    +E        F  V  ++  AL  LPY+ F +  EV
Sbjct: 79  ARDLLRKTRDGSKIDQAMRGDAFLDE--------FAGVNRQIHLALDALPYNTFHMPQEV 138

Query: 133 QEQVDLVRAQLRRASNK-------------YESMSNPAEKKLQTRGSVKWMINN--DVKS 192
           QEQV LV +Q +RAS +             +    NP++  L TR S K  ++   D+K+
Sbjct: 139 QEQVALVHSQFQRASTRTDPPDTQLSMDLAWALTDNPSDPALLTRISHKLQLHTMADMKN 198

Query: 193 IS-SVDDGDVESQHHPRNCNYPTSFDSVNSCFDESSS--VVHSDMEDVLASKSQD-EVRK 252
            S ++ +  + +   P  C      D ++S   +     V      D L ++S   + R 
Sbjct: 199 ESIALHNMVISTAGEPDGC-----VDQMSSLLKKLKDCVVTEDHANDALTTRSASIKHRS 258

Query: 253 PIEVDIPENFLCPISFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILT 312
           PI   IP+ F CPIS ELM DPVI S+GQTYERS +Q W+D G++TCPKTQ+ L    LT
Sbjct: 259 PI---IPDEFRCPISLELMQDPVIVSSGQTYERSCIQKWLDSGHKTCPKTQQPLSHTSLT 318

Query: 313 PNFLMRKLIAEWCEEHNVKLEEGLTSRKFKKYRSFEDDCRRTLPIKTLVRHLSFGSIQEQ 372
           PNF+++ LI++WCE + ++L +   + + KK     D     L   +L+  L  G+  EQ
Sbjct: 319 PNFVLKSLISQWCEANGIELPKNKQNSRDKKAAKSSDYDHAGL--VSLMNRLRSGNQDEQ 378

Query: 373 KIAVTEIRKLSKSSSDHRVEIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLHEQNKR 432
           + A  EIR L+K + ++R+ IAEAGAIP LVNLL+S D  TQE+A++ +LNLS+HE NK 
Sbjct: 379 RAAAGEIRLLAKRNVNNRICIAEAGAIPLLVNLLSSSDPRTQEHAVTALLNLSIHENNKA 438

Query: 433 LVMLSGAVSYISKVLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGS 492
            ++ S A+  I +VLK GSME RE AAAT++SLS+ DENK  IGA+G IP L+ +L  GS
Sbjct: 439 SIVDSHAIPKIVEVLKTGSMETRENAAATLFSLSVVDENKVTIGAAGAIPPLINLLCDGS 498

Query: 493 PRGQKDAAGALLNLCMYQGNKGRALKAGIVRPLLKILSDPNGSLVDDALYIMSVLCGHPE 552
           PRG+KDAA A+ NLC+YQGNK RA+KAGIV  L+  L DP G ++D+AL ++S+L G+PE
Sbjct: 499 PRGKKDAATAIFNLCIYQGNKVRAVKAGIVIHLMNFLVDPTGGMIDEALSLLSILAGNPE 558

Query: 553 AKAAMANANTLLVLTDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLA 612
            K  +A +  +  L +V+K GS R++ENAAA+L  LC  D E+       G   +L +L+
Sbjct: 559 GKIVIARSEPIPPLVEVIKTGSPRNRENAAAILWLLCSADTEQTLAAKAAGVEDALKELS 601

Query: 613 ENGTGRARRKAASLLEQLRKS 615
           E GT RA+RKA+S+LE + ++
Sbjct: 619 ETGTDRAKRKASSILELMHQA 601

BLAST of Bhi12G000764 vs. ExPASy Swiss-Prot
Match: Q9SNC6 (U-box domain-containing protein 13 OS=Arabidopsis thaliana OX=3702 GN=PUB13 PE=1 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 1.0e-103
Identity = 235/625 (37.60%), Postives = 376/625 (60.16%), Query Frame = 0

Query: 13  MKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSSLDCLSEVVGAIQA 72
           +KK C +L RR+ LL+ + EEI        E+ E +++D       +L  L  +  A+ +
Sbjct: 29  VKKLCYNLARRLKLLVPMFEEIR-------ESNEPISED-------TLKTLMNLKEAMCS 88

Query: 73  AKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSNLPYDHFCVSDEV 132
           AK      L F     +       E+ T KL+     V+ +LE +LS +PY+   +SDEV
Sbjct: 89  AKDY----LKFCSQGSKIYLVMEREQVTSKLM----EVSVKLEQSLSQIPYEELDISDEV 148

Query: 133 QEQVDLVRAQLRRASNKYESMSNPAEKKLQTR-------GSVKWMINNDVKSISSVDDGD 192
           +EQV+LV +Q RRA  + +   +   + LQ+         + + ++    K +  ++  D
Sbjct: 149 REQVELVLSQFRRAKGRVDVSDDELYEDLQSLCNKSSDVDAYQPVLERVAKKLHLMEIPD 208

Query: 193 VESQHHPRNCNYPTSFDSVNSCFDESSSVVHSDMEDVLASKSQDEVRKPIEVD------- 252
           +  +    +    +S   V    +E + V+   ++D + ++  +   + + V+       
Sbjct: 209 LAQESVALHEMVASSGGDVGENIEEMAMVLKM-IKDFVQTEDDNGEEQKVGVNSRSNGQT 268

Query: 253 ----------IPENFLCPISFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQ 312
                     IP++F CPIS E+M DPVI S+GQTYER+ ++ WI+ G+ TCPKTQ+ L 
Sbjct: 269 STAASQKIPVIPDDFRCPISLEMMRDPVIVSSGQTYERTCIEKWIEGGHSTCPKTQQALT 328

Query: 313 TLILTPNFLMRKLIAEWCEEHNVKLEEGLTSRKFKKYRSFEDDCRRTLPIKTLVRHLSFG 372
           +  LTPN+++R LIA+WCE ++++  +  +S + +K  SF         I+ L+  L++G
Sbjct: 329 STTLTPNYVLRSLIAQWCEANDIEPPKPPSSLRPRKVSSFSSPAEAN-KIEDLMWRLAYG 388

Query: 373 SIQEQKIAVTEIRKLSKSSSDHRVEIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLH 432
           + ++Q+ A  EIR L+K ++D+RV IAEAGAIP LV LL++ D   QE++++ +LNLS+ 
Sbjct: 389 NPEDQRSAAGEIRLLAKRNADNRVAIAEAGAIPLLVGLLSTPDSRIQEHSVTALLNLSIC 448

Query: 433 EQNKRLVMLSGAVSYISKVLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEI 492
           E NK  ++ +GA+  I +VLK GSME RE AAAT++SLS+ DENK  IGA G IP L+ +
Sbjct: 449 ENNKGAIVSAGAIPGIVQVLKKGSMEARENAAATLFSLSVIDENKVTIGALGAIPPLVVL 508

Query: 493 LEIGSPRGQKDAAGALLNLCMYQGNKGRALKAGIVRPLLKILSDPNGSLVDDALYIMSVL 552
           L  G+ RG+KDAA AL NLC+YQGNKG+A++AG++  L ++L++P   +VD+AL I+++L
Sbjct: 509 LNEGTQRGKKDAATALFNLCIYQGNKGKAIRAGVIPTLTRLLTEPGSGMVDEALAILAIL 568

Query: 553 CGHPEAKAAMANANTLLVLTDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMAS 612
             HPE KA + +++ +  L + ++ GS R++ENAAAVL+ LC GD + L    +LG M  
Sbjct: 569 SSHPEGKAIIGSSDAVPSLVEFIRTGSPRNRENAAAVLVHLCSGDPQHLVEAQKLGLMGP 628

Query: 613 LMKLAENGTGRARRKAASLLEQLRK 614
           L+ LA NGT R +RKAA LLE++ +
Sbjct: 629 LIDLAGNGTDRGKRKAAQLLERISR 629

BLAST of Bhi12G000764 vs. NCBI nr
Match: XP_038875664.1 (U-box domain-containing protein 11-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1186.0 bits (3067), Expect = 0.0e+00
Identity = 614/614 (100.00%), Postives = 614/614 (100.00%), Query Frame = 0

Query: 1   MSGRSIAGAGDVMKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSSL 60
           MSGRSIAGAGDVMKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSSL
Sbjct: 1   MSGRSIAGAGDVMKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSSL 60

Query: 61  DCLSEVVGAIQAAKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSN 120
           DCLSEVVGAIQAAKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSN
Sbjct: 61  DCLSEVVGAIQAAKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSN 120

Query: 121 LPYDHFCVSDEVQEQVDLVRAQLRRASNKYESMSNPAEKKLQTRGSVKWMINNDVKSISS 180
           LPYDHFCVSDEVQEQVDLVRAQLRRASNKYESMSNPAEKKLQTRGSVKWMINNDVKSISS
Sbjct: 121 LPYDHFCVSDEVQEQVDLVRAQLRRASNKYESMSNPAEKKLQTRGSVKWMINNDVKSISS 180

Query: 181 VDDGDVESQHHPRNCNYPTSFDSVNSCFDESSSVVHSDMEDVLASKSQDEVRKPIEVDIP 240
           VDDGDVESQHHPRNCNYPTSFDSVNSCFDESSSVVHSDMEDVLASKSQDEVRKPIEVDIP
Sbjct: 181 VDDGDVESQHHPRNCNYPTSFDSVNSCFDESSSVVHSDMEDVLASKSQDEVRKPIEVDIP 240

Query: 241 ENFLCPISFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPNFLMRK 300
           ENFLCPISFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPNFLMRK
Sbjct: 241 ENFLCPISFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPNFLMRK 300

Query: 301 LIAEWCEEHNVKLEEGLTSRKFKKYRSFEDDCRRTLPIKTLVRHLSFGSIQEQKIAVTEI 360
           LIAEWCEEHNVKLEEGLTSRKFKKYRSFEDDCRRTLPIKTLVRHLSFGSIQEQKIAVTEI
Sbjct: 301 LIAEWCEEHNVKLEEGLTSRKFKKYRSFEDDCRRTLPIKTLVRHLSFGSIQEQKIAVTEI 360

Query: 361 RKLSKSSSDHRVEIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLHEQNKRLVMLSGA 420
           RKLSKSSSDHRVEIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLHEQNKRLVMLSGA
Sbjct: 361 RKLSKSSSDHRVEIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLHEQNKRLVMLSGA 420

Query: 421 VSYISKVLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSPRGQKDA 480
           VSYISKVLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSPRGQKDA
Sbjct: 421 VSYISKVLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSPRGQKDA 480

Query: 481 AGALLNLCMYQGNKGRALKAGIVRPLLKILSDPNGSLVDDALYIMSVLCGHPEAKAAMAN 540
           AGALLNLCMYQGNKGRALKAGIVRPLLKILSDPNGSLVDDALYIMSVLCGHPEAKAAMAN
Sbjct: 481 AGALLNLCMYQGNKGRALKAGIVRPLLKILSDPNGSLVDDALYIMSVLCGHPEAKAAMAN 540

Query: 541 ANTLLVLTDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLAENGTGRA 600
           ANTLLVLTDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLAENGTGRA
Sbjct: 541 ANTLLVLTDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLAENGTGRA 600

Query: 601 RRKAASLLEQLRKS 615
           RRKAASLLEQLRKS
Sbjct: 601 RRKAASLLEQLRKS 614

BLAST of Bhi12G000764 vs. NCBI nr
Match: XP_038875663.1 (U-box domain-containing protein 11-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1186.0 bits (3067), Expect = 0.0e+00
Identity = 614/614 (100.00%), Postives = 614/614 (100.00%), Query Frame = 0

Query: 1   MSGRSIAGAGDVMKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSSL 60
           MSGRSIAGAGDVMKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSSL
Sbjct: 28  MSGRSIAGAGDVMKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSSL 87

Query: 61  DCLSEVVGAIQAAKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSN 120
           DCLSEVVGAIQAAKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSN
Sbjct: 88  DCLSEVVGAIQAAKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSN 147

Query: 121 LPYDHFCVSDEVQEQVDLVRAQLRRASNKYESMSNPAEKKLQTRGSVKWMINNDVKSISS 180
           LPYDHFCVSDEVQEQVDLVRAQLRRASNKYESMSNPAEKKLQTRGSVKWMINNDVKSISS
Sbjct: 148 LPYDHFCVSDEVQEQVDLVRAQLRRASNKYESMSNPAEKKLQTRGSVKWMINNDVKSISS 207

Query: 181 VDDGDVESQHHPRNCNYPTSFDSVNSCFDESSSVVHSDMEDVLASKSQDEVRKPIEVDIP 240
           VDDGDVESQHHPRNCNYPTSFDSVNSCFDESSSVVHSDMEDVLASKSQDEVRKPIEVDIP
Sbjct: 208 VDDGDVESQHHPRNCNYPTSFDSVNSCFDESSSVVHSDMEDVLASKSQDEVRKPIEVDIP 267

Query: 241 ENFLCPISFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPNFLMRK 300
           ENFLCPISFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPNFLMRK
Sbjct: 268 ENFLCPISFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPNFLMRK 327

Query: 301 LIAEWCEEHNVKLEEGLTSRKFKKYRSFEDDCRRTLPIKTLVRHLSFGSIQEQKIAVTEI 360
           LIAEWCEEHNVKLEEGLTSRKFKKYRSFEDDCRRTLPIKTLVRHLSFGSIQEQKIAVTEI
Sbjct: 328 LIAEWCEEHNVKLEEGLTSRKFKKYRSFEDDCRRTLPIKTLVRHLSFGSIQEQKIAVTEI 387

Query: 361 RKLSKSSSDHRVEIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLHEQNKRLVMLSGA 420
           RKLSKSSSDHRVEIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLHEQNKRLVMLSGA
Sbjct: 388 RKLSKSSSDHRVEIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLHEQNKRLVMLSGA 447

Query: 421 VSYISKVLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSPRGQKDA 480
           VSYISKVLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSPRGQKDA
Sbjct: 448 VSYISKVLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSPRGQKDA 507

Query: 481 AGALLNLCMYQGNKGRALKAGIVRPLLKILSDPNGSLVDDALYIMSVLCGHPEAKAAMAN 540
           AGALLNLCMYQGNKGRALKAGIVRPLLKILSDPNGSLVDDALYIMSVLCGHPEAKAAMAN
Sbjct: 508 AGALLNLCMYQGNKGRALKAGIVRPLLKILSDPNGSLVDDALYIMSVLCGHPEAKAAMAN 567

Query: 541 ANTLLVLTDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLAENGTGRA 600
           ANTLLVLTDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLAENGTGRA
Sbjct: 568 ANTLLVLTDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLAENGTGRA 627

Query: 601 RRKAASLLEQLRKS 615
           RRKAASLLEQLRKS
Sbjct: 628 RRKAASLLEQLRKS 641

BLAST of Bhi12G000764 vs. NCBI nr
Match: XP_038875665.1 (U-box domain-containing protein 11-like isoform X3 [Benincasa hispida])

HSP 1 Score: 1163.3 bits (3008), Expect = 0.0e+00
Identity = 602/602 (100.00%), Postives = 602/602 (100.00%), Query Frame = 0

Query: 13  MKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSSLDCLSEVVGAIQA 72
           MKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSSLDCLSEVVGAIQA
Sbjct: 1   MKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSSLDCLSEVVGAIQA 60

Query: 73  AKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSNLPYDHFCVSDEV 132
           AKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSNLPYDHFCVSDEV
Sbjct: 61  AKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSNLPYDHFCVSDEV 120

Query: 133 QEQVDLVRAQLRRASNKYESMSNPAEKKLQTRGSVKWMINNDVKSISSVDDGDVESQHHP 192
           QEQVDLVRAQLRRASNKYESMSNPAEKKLQTRGSVKWMINNDVKSISSVDDGDVESQHHP
Sbjct: 121 QEQVDLVRAQLRRASNKYESMSNPAEKKLQTRGSVKWMINNDVKSISSVDDGDVESQHHP 180

Query: 193 RNCNYPTSFDSVNSCFDESSSVVHSDMEDVLASKSQDEVRKPIEVDIPENFLCPISFELM 252
           RNCNYPTSFDSVNSCFDESSSVVHSDMEDVLASKSQDEVRKPIEVDIPENFLCPISFELM
Sbjct: 181 RNCNYPTSFDSVNSCFDESSSVVHSDMEDVLASKSQDEVRKPIEVDIPENFLCPISFELM 240

Query: 253 LDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPNFLMRKLIAEWCEEHNVK 312
           LDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPNFLMRKLIAEWCEEHNVK
Sbjct: 241 LDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPNFLMRKLIAEWCEEHNVK 300

Query: 313 LEEGLTSRKFKKYRSFEDDCRRTLPIKTLVRHLSFGSIQEQKIAVTEIRKLSKSSSDHRV 372
           LEEGLTSRKFKKYRSFEDDCRRTLPIKTLVRHLSFGSIQEQKIAVTEIRKLSKSSSDHRV
Sbjct: 301 LEEGLTSRKFKKYRSFEDDCRRTLPIKTLVRHLSFGSIQEQKIAVTEIRKLSKSSSDHRV 360

Query: 373 EIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLHEQNKRLVMLSGAVSYISKVLKVGS 432
           EIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLHEQNKRLVMLSGAVSYISKVLKVGS
Sbjct: 361 EIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLHEQNKRLVMLSGAVSYISKVLKVGS 420

Query: 433 MEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSPRGQKDAAGALLNLCMYQG 492
           MEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSPRGQKDAAGALLNLCMYQG
Sbjct: 421 MEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSPRGQKDAAGALLNLCMYQG 480

Query: 493 NKGRALKAGIVRPLLKILSDPNGSLVDDALYIMSVLCGHPEAKAAMANANTLLVLTDVLK 552
           NKGRALKAGIVRPLLKILSDPNGSLVDDALYIMSVLCGHPEAKAAMANANTLLVLTDVLK
Sbjct: 481 NKGRALKAGIVRPLLKILSDPNGSLVDDALYIMSVLCGHPEAKAAMANANTLLVLTDVLK 540

Query: 553 MGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLAENGTGRARRKAASLLEQLR 612
           MGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLAENGTGRARRKAASLLEQLR
Sbjct: 541 MGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLAENGTGRARRKAASLLEQLR 600

Query: 613 KS 615
           KS
Sbjct: 601 KS 602

BLAST of Bhi12G000764 vs. NCBI nr
Match: XP_008467149.1 (PREDICTED: U-box domain-containing protein 11 [Cucumis melo])

HSP 1 Score: 1028.9 bits (2659), Expect = 1.8e-296
Identity = 537/616 (87.18%), Postives = 576/616 (93.51%), Query Frame = 0

Query: 1   MSGRSIAGAGDVMKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSS- 60
           MSGRSIAG GD MKKDCTDLIRRIALLIHLAEEITNFCSGSG+NFE  NDDGSS+S SS 
Sbjct: 28  MSGRSIAGTGDAMKKDCTDLIRRIALLIHLAEEITNFCSGSGDNFEKSNDDGSSTSLSSW 87

Query: 61  LDCLSEVVGAIQAAKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALS 120
           LDCLSEVVGAIQAAKRLLY ALTFS YDEEGC A STEE TKKLVLQF++VTTRLETALS
Sbjct: 88  LDCLSEVVGAIQAAKRLLYTALTFSAYDEEGC-APSTEEGTKKLVLQFKHVTTRLETALS 147

Query: 121 NLPYDHFCVSDEVQEQVDLVRAQLRRASNKYESMSNPAEKKLQTRGSVKWMINNDVKSIS 180
           NLPYD FCVSDEVQEQVDLVRAQLRRAS+KYESMSNPAEKKLQ R SVKWMINN+V+S++
Sbjct: 148 NLPYDQFCVSDEVQEQVDLVRAQLRRASHKYESMSNPAEKKLQARSSVKWMINNEVRSMA 207

Query: 181 SVDDGDVESQHHPRNCNYPTSFDSVNSCFDESSSVVHSDMEDVLASKSQDEVRKPIEVDI 240
           SVDDGD ESQH PRN ++  S DSVNSCFDE SSVVHSD EDV+AS+SQDEV+KP+E++I
Sbjct: 208 SVDDGD-ESQHRPRNRDHLASLDSVNSCFDECSSVVHSDTEDVVASRSQDEVKKPLEIEI 267

Query: 241 PENFLCPISFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPNFLMR 300
           PENFLCPIS+ELMLDPVI STGQTYERSN+Q WIDRGNR CPKTQEQLQ LILTPNF+MR
Sbjct: 268 PENFLCPISYELMLDPVIISTGQTYERSNIQKWIDRGNRICPKTQEQLQALILTPNFIMR 327

Query: 301 KLIAEWCEEHNVKLEEGLTSRKFKKYRSFEDDCRRT-LPIKTLVRHLSFGSIQEQKIAVT 360
           KLI EWCEEHNVKLEEGLT+ K KK RS EDDCRRT LPIKTLVRHLSFGS+QEQKIAVT
Sbjct: 328 KLIYEWCEEHNVKLEEGLTNGKLKKCRSSEDDCRRTPLPIKTLVRHLSFGSVQEQKIAVT 387

Query: 361 EIRKLSKSSSDHRVEIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLHEQNKRLVMLS 420
           EIR+LSKSSS+HRVEIAEAGAIPQLVNLL+S+D++TQENA+SCILNLSLHEQNKRL+MLS
Sbjct: 388 EIRQLSKSSSEHRVEIAEAGAIPQLVNLLSSKDVITQENAISCILNLSLHEQNKRLIMLS 447

Query: 421 GAVSYISKVLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSPRGQK 480
           GAVSYIS+VLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEIL+IG+PRGQK
Sbjct: 448 GAVSYISQVLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILDIGTPRGQK 507

Query: 481 DAAGALLNLCMYQGNKGRALKAGIVRPLLKILSDPNGSLVDDALYIMSVLCGHPEAKAAM 540
           DAAGALLNLCMYQGNKGRAL AGIV+PLLK+LSD NGSLVDDALYIMS+LCGHP+AKAAM
Sbjct: 508 DAAGALLNLCMYQGNKGRALNAGIVKPLLKMLSDSNGSLVDDALYIMSILCGHPDAKAAM 567

Query: 541 ANANTLLVLTDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLAENGTG 600
            NAN+LLVLTDVLK GS RSKENAAAVLLA CKGD EKLEWLTRLGAMA LMKLAENGTG
Sbjct: 568 GNANSLLVLTDVLKTGSPRSKENAAAVLLAFCKGDREKLEWLTRLGAMAPLMKLAENGTG 627

Query: 601 RARRKAASLLEQLRKS 615
           RARRKAASLL+QLRKS
Sbjct: 628 RARRKAASLLDQLRKS 641

BLAST of Bhi12G000764 vs. NCBI nr
Match: KAE8648611.1 (hypothetical protein Csa_009171 [Cucumis sativus])

HSP 1 Score: 1023.1 bits (2644), Expect = 1.0e-294
Identity = 535/617 (86.71%), Postives = 573/617 (92.87%), Query Frame = 0

Query: 1   MSGRSIAGAGDVMKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSS- 60
           MSGRSIAGAGD MKKDCTDLIRRIALL+HLAEEITNFCSGS +NFE  NDDGSSSSS S 
Sbjct: 28  MSGRSIAGAGDTMKKDCTDLIRRIALLVHLAEEITNFCSGSWDNFEKSNDDGSSSSSLSS 87

Query: 61  -LDCLSEVVGAIQAAKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETAL 120
            LDCLSEVVGAIQAAKRLLY ALTFS  DEEGC  TSTEEATKKLVLQF++VTT LETAL
Sbjct: 88  WLDCLSEVVGAIQAAKRLLYTALTFSPNDEEGC-VTSTEEATKKLVLQFKHVTTILETAL 147

Query: 121 SNLPYDHFCVSDEVQEQVDLVRAQLRRASNKYESMSNPAEKKLQTRGSVKWMINNDVKSI 180
           SNLPYD FCVSDEVQEQVDLVRAQLRRAS+KYESMSNPAEKKLQ R S+KWMINNDV+S+
Sbjct: 148 SNLPYDKFCVSDEVQEQVDLVRAQLRRASHKYESMSNPAEKKLQARSSIKWMINNDVRSM 207

Query: 181 SSVDDGDVESQHHPRNCNYPTSFDSVNSCFDESSSVVHSDMEDVLASKSQDEVRKPIEVD 240
           SSVDDGD ESQH PRN +Y TS DSVNSCFDE SSVVHSDMEDV+AS+SQDEV+K +E +
Sbjct: 208 SSVDDGD-ESQHRPRNRDYLTSLDSVNSCFDECSSVVHSDMEDVVASRSQDEVKKHLETE 267

Query: 241 IPENFLCPISFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPNFLM 300
           IPENFLCPIS+ELMLDPVI STGQTYERSN+QNWIDRGNR CPKTQEQLQ LILTPNF+M
Sbjct: 268 IPENFLCPISYELMLDPVIVSTGQTYERSNIQNWIDRGNRICPKTQEQLQALILTPNFIM 327

Query: 301 RKLIAEWCEEHNVKLEEGLTSRKFKKYRSFEDDCRRT-LPIKTLVRHLSFGSIQEQKIAV 360
           RKLI EWCEEHNVKLEEGLT++K KK RSFEDDCRRT LPI TLVRHLSFGS+QEQK AV
Sbjct: 328 RKLIYEWCEEHNVKLEEGLTNKKLKKCRSFEDDCRRTPLPINTLVRHLSFGSVQEQKTAV 387

Query: 361 TEIRKLSKSSSDHRVEIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLHEQNKRLVML 420
           TEIR+LSKSSSDHRVEIAEAGAIPQLVNLLTS+D++TQENA+SCILNLSLHEQNKRL+ML
Sbjct: 388 TEIRQLSKSSSDHRVEIAEAGAIPQLVNLLTSKDVITQENAISCILNLSLHEQNKRLIML 447

Query: 421 SGAVSYISKVLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSPRGQ 480
           SGAVSYIS+VLKVGSMEGRECAAATIYSLSLADENKA+IGAS VIPDL+EIL+IGSPRGQ
Sbjct: 448 SGAVSYISQVLKVGSMEGRECAAATIYSLSLADENKAVIGASDVIPDLIEILDIGSPRGQ 507

Query: 481 KDAAGALLNLCMYQGNKGRALKAGIVRPLLKILSDPNGSLVDDALYIMSVLCGHPEAKAA 540
           KDAAGALLNLCMYQGNKGRALKAGIV+PLLK+LSD NGSLVDDALYIMS+LCGHP+AKA 
Sbjct: 508 KDAAGALLNLCMYQGNKGRALKAGIVKPLLKMLSDSNGSLVDDALYIMSILCGHPDAKAT 567

Query: 541 MANANTLLVLTDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLAENGT 600
           M NAN+LLVLTDVLK GS RSKENAAAVLLA CKGD EKLEWLTRLGA+A LMKL ENGT
Sbjct: 568 MGNANSLLVLTDVLKTGSPRSKENAAAVLLAFCKGDREKLEWLTRLGAIAPLMKLGENGT 627

Query: 601 GRARRKAASLLEQLRKS 615
           GRARRKAA+LL+QL KS
Sbjct: 628 GRARRKAATLLDQLGKS 642

BLAST of Bhi12G000764 vs. ExPASy TrEMBL
Match: A0A1S3CSU4 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103504572 PE=4 SV=1)

HSP 1 Score: 1028.9 bits (2659), Expect = 8.9e-297
Identity = 537/616 (87.18%), Postives = 576/616 (93.51%), Query Frame = 0

Query: 1   MSGRSIAGAGDVMKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSS- 60
           MSGRSIAG GD MKKDCTDLIRRIALLIHLAEEITNFCSGSG+NFE  NDDGSS+S SS 
Sbjct: 28  MSGRSIAGTGDAMKKDCTDLIRRIALLIHLAEEITNFCSGSGDNFEKSNDDGSSTSLSSW 87

Query: 61  LDCLSEVVGAIQAAKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALS 120
           LDCLSEVVGAIQAAKRLLY ALTFS YDEEGC A STEE TKKLVLQF++VTTRLETALS
Sbjct: 88  LDCLSEVVGAIQAAKRLLYTALTFSAYDEEGC-APSTEEGTKKLVLQFKHVTTRLETALS 147

Query: 121 NLPYDHFCVSDEVQEQVDLVRAQLRRASNKYESMSNPAEKKLQTRGSVKWMINNDVKSIS 180
           NLPYD FCVSDEVQEQVDLVRAQLRRAS+KYESMSNPAEKKLQ R SVKWMINN+V+S++
Sbjct: 148 NLPYDQFCVSDEVQEQVDLVRAQLRRASHKYESMSNPAEKKLQARSSVKWMINNEVRSMA 207

Query: 181 SVDDGDVESQHHPRNCNYPTSFDSVNSCFDESSSVVHSDMEDVLASKSQDEVRKPIEVDI 240
           SVDDGD ESQH PRN ++  S DSVNSCFDE SSVVHSD EDV+AS+SQDEV+KP+E++I
Sbjct: 208 SVDDGD-ESQHRPRNRDHLASLDSVNSCFDECSSVVHSDTEDVVASRSQDEVKKPLEIEI 267

Query: 241 PENFLCPISFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPNFLMR 300
           PENFLCPIS+ELMLDPVI STGQTYERSN+Q WIDRGNR CPKTQEQLQ LILTPNF+MR
Sbjct: 268 PENFLCPISYELMLDPVIISTGQTYERSNIQKWIDRGNRICPKTQEQLQALILTPNFIMR 327

Query: 301 KLIAEWCEEHNVKLEEGLTSRKFKKYRSFEDDCRRT-LPIKTLVRHLSFGSIQEQKIAVT 360
           KLI EWCEEHNVKLEEGLT+ K KK RS EDDCRRT LPIKTLVRHLSFGS+QEQKIAVT
Sbjct: 328 KLIYEWCEEHNVKLEEGLTNGKLKKCRSSEDDCRRTPLPIKTLVRHLSFGSVQEQKIAVT 387

Query: 361 EIRKLSKSSSDHRVEIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLHEQNKRLVMLS 420
           EIR+LSKSSS+HRVEIAEAGAIPQLVNLL+S+D++TQENA+SCILNLSLHEQNKRL+MLS
Sbjct: 388 EIRQLSKSSSEHRVEIAEAGAIPQLVNLLSSKDVITQENAISCILNLSLHEQNKRLIMLS 447

Query: 421 GAVSYISKVLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSPRGQK 480
           GAVSYIS+VLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEIL+IG+PRGQK
Sbjct: 448 GAVSYISQVLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILDIGTPRGQK 507

Query: 481 DAAGALLNLCMYQGNKGRALKAGIVRPLLKILSDPNGSLVDDALYIMSVLCGHPEAKAAM 540
           DAAGALLNLCMYQGNKGRAL AGIV+PLLK+LSD NGSLVDDALYIMS+LCGHP+AKAAM
Sbjct: 508 DAAGALLNLCMYQGNKGRALNAGIVKPLLKMLSDSNGSLVDDALYIMSILCGHPDAKAAM 567

Query: 541 ANANTLLVLTDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLAENGTG 600
            NAN+LLVLTDVLK GS RSKENAAAVLLA CKGD EKLEWLTRLGAMA LMKLAENGTG
Sbjct: 568 GNANSLLVLTDVLKTGSPRSKENAAAVLLAFCKGDREKLEWLTRLGAMAPLMKLAENGTG 627

Query: 601 RARRKAASLLEQLRKS 615
           RARRKAASLL+QLRKS
Sbjct: 628 RARRKAASLLDQLRKS 641

BLAST of Bhi12G000764 vs. ExPASy TrEMBL
Match: A0A6J1K2B7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111489474 PE=4 SV=1)

HSP 1 Score: 936.8 bits (2420), Expect = 4.6e-269
Identity = 495/619 (79.97%), Postives = 541/619 (87.40%), Query Frame = 0

Query: 1   MSGRSIAGAGDVMKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSSL 60
           MSGRSI GAGDVMKKDCTDLIRRIALLIHLAEEITNFC G  + FEAL D  SSSSSS L
Sbjct: 61  MSGRSITGAGDVMKKDCTDLIRRIALLIHLAEEITNFCGGFRDKFEALTDSISSSSSSCL 120

Query: 61  DCLSEVVGAIQAAKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSN 120
           DCLSEV+GAIQAAKRLLY A+TFSVYD++    TSTE ATKKLVLQF YVT+RLETALSN
Sbjct: 121 DCLSEVIGAIQAAKRLLYSAMTFSVYDDDEVSLTSTEGATKKLVLQFHYVTSRLETALSN 180

Query: 121 LPYDHFCVSDEVQEQVDLVRAQLRRASNKYESMSNPAEKKLQTRGSVKWMINNDVKSISS 180
           LPYDHFCV+DEVQEQVDLVRAQL RAS  YESMS+P  KKL+  GSVK MI +DVK++SS
Sbjct: 181 LPYDHFCVADEVQEQVDLVRAQLMRASKNYESMSDPTHKKLEATGSVKAMIFHDVKTMSS 240

Query: 181 VDDGDVESQHHPRNCNYPTSFDS-----VNSCFDESSSVVHSDMEDVLASKSQDEVRKPI 240
           VD+GD +SQH P N +    FDS      N CF+E SS VHS+MEDVL+ KSQDEV K  
Sbjct: 241 VDNGDPDSQHCPPNRDDLIRFDSDEIKNSNPCFNECSS-VHSEMEDVLSIKSQDEVGKLD 300

Query: 241 EVDIPENFLCPISFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPN 300
            ++IPENFLC IS ELM+DPVI STGQTYER N+Q WIDRGN TCPKTQEQLQ+LILTPN
Sbjct: 301 VIEIPENFLCSISLELMIDPVIVSTGQTYERYNIQKWIDRGNTTCPKTQEQLQSLILTPN 360

Query: 301 FLMRKLIAEWCEEHNVKLEEGLTSRKFKKYRSFEDDCRRTLPIKTLVRHLSFGSIQEQKI 360
           F MR LI+EWC EHNV LE+GLT+RK KKYRSFED CRRTLPIKTLVRHLSFGS+QEQK 
Sbjct: 361 FAMRNLISEWCGEHNVNLEKGLTNRKLKKYRSFEDGCRRTLPIKTLVRHLSFGSVQEQKA 420

Query: 361 AVTEIRKLSKSSSDHRVEIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLHEQNKRLV 420
           AVTEIR+LSKSSSDHRVEIA+AGAIPQLV LLTSED+ TQENA+SCILNLSLHE NKRL+
Sbjct: 421 AVTEIRQLSKSSSDHRVEIAKAGAIPQLVILLTSEDVATQENAISCILNLSLHEPNKRLI 480

Query: 421 MLSGAVSYISKVLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSPR 480
           ML GA SYIS+VLK GSMEGRECAA TIYSLSLADENKA+IGASGVIPDL+EIL+IGSPR
Sbjct: 481 MLHGAFSYISQVLKFGSMEGRECAAGTIYSLSLADENKAMIGASGVIPDLIEILQIGSPR 540

Query: 481 GQKDAAGALLNLCMYQGNKGRALKAGIVRPLLKILSDPNGSLVDDALYIMSVLCGHPEAK 540
           GQKDAAGALLNLCMYQGNKGRA +AGIV+ LLK+LSD NG+LVDDALYIMSVLC HPEAK
Sbjct: 541 GQKDAAGALLNLCMYQGNKGRAFRAGIVKLLLKMLSDSNGTLVDDALYIMSVLCSHPEAK 600

Query: 541 AAMANANTLLVLTDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLAEN 600
           AAM NAN+LLVLT+VLK GSSRS+ENA AVLLALCKGDWEKLEWLTRLGA   LMKL+E+
Sbjct: 601 AAMGNANSLLVLTNVLKKGSSRSRENAVAVLLALCKGDWEKLEWLTRLGAAVPLMKLSED 660

Query: 601 GTGRARRKAASLLEQLRKS 615
           GT RARRKAASLL+QLRKS
Sbjct: 661 GTERARRKAASLLDQLRKS 678

BLAST of Bhi12G000764 vs. ExPASy TrEMBL
Match: A0A6J1JYM0 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111489474 PE=4 SV=1)

HSP 1 Score: 936.8 bits (2420), Expect = 4.6e-269
Identity = 495/619 (79.97%), Postives = 541/619 (87.40%), Query Frame = 0

Query: 1   MSGRSIAGAGDVMKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSSL 60
           MSGRSI GAGDVMKKDCTDLIRRIALLIHLAEEITNFC G  + FEAL D  SSSSSS L
Sbjct: 56  MSGRSITGAGDVMKKDCTDLIRRIALLIHLAEEITNFCGGFRDKFEALTDSISSSSSSCL 115

Query: 61  DCLSEVVGAIQAAKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSN 120
           DCLSEV+GAIQAAKRLLY A+TFSVYD++    TSTE ATKKLVLQF YVT+RLETALSN
Sbjct: 116 DCLSEVIGAIQAAKRLLYSAMTFSVYDDDEVSLTSTEGATKKLVLQFHYVTSRLETALSN 175

Query: 121 LPYDHFCVSDEVQEQVDLVRAQLRRASNKYESMSNPAEKKLQTRGSVKWMINNDVKSISS 180
           LPYDHFCV+DEVQEQVDLVRAQL RAS  YESMS+P  KKL+  GSVK MI +DVK++SS
Sbjct: 176 LPYDHFCVADEVQEQVDLVRAQLMRASKNYESMSDPTHKKLEATGSVKAMIFHDVKTMSS 235

Query: 181 VDDGDVESQHHPRNCNYPTSFDS-----VNSCFDESSSVVHSDMEDVLASKSQDEVRKPI 240
           VD+GD +SQH P N +    FDS      N CF+E SS VHS+MEDVL+ KSQDEV K  
Sbjct: 236 VDNGDPDSQHCPPNRDDLIRFDSDEIKNSNPCFNECSS-VHSEMEDVLSIKSQDEVGKLD 295

Query: 241 EVDIPENFLCPISFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPN 300
            ++IPENFLC IS ELM+DPVI STGQTYER N+Q WIDRGN TCPKTQEQLQ+LILTPN
Sbjct: 296 VIEIPENFLCSISLELMIDPVIVSTGQTYERYNIQKWIDRGNTTCPKTQEQLQSLILTPN 355

Query: 301 FLMRKLIAEWCEEHNVKLEEGLTSRKFKKYRSFEDDCRRTLPIKTLVRHLSFGSIQEQKI 360
           F MR LI+EWC EHNV LE+GLT+RK KKYRSFED CRRTLPIKTLVRHLSFGS+QEQK 
Sbjct: 356 FAMRNLISEWCGEHNVNLEKGLTNRKLKKYRSFEDGCRRTLPIKTLVRHLSFGSVQEQKA 415

Query: 361 AVTEIRKLSKSSSDHRVEIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLHEQNKRLV 420
           AVTEIR+LSKSSSDHRVEIA+AGAIPQLV LLTSED+ TQENA+SCILNLSLHE NKRL+
Sbjct: 416 AVTEIRQLSKSSSDHRVEIAKAGAIPQLVILLTSEDVATQENAISCILNLSLHEPNKRLI 475

Query: 421 MLSGAVSYISKVLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSPR 480
           ML GA SYIS+VLK GSMEGRECAA TIYSLSLADENKA+IGASGVIPDL+EIL+IGSPR
Sbjct: 476 MLHGAFSYISQVLKFGSMEGRECAAGTIYSLSLADENKAMIGASGVIPDLIEILQIGSPR 535

Query: 481 GQKDAAGALLNLCMYQGNKGRALKAGIVRPLLKILSDPNGSLVDDALYIMSVLCGHPEAK 540
           GQKDAAGALLNLCMYQGNKGRA +AGIV+ LLK+LSD NG+LVDDALYIMSVLC HPEAK
Sbjct: 536 GQKDAAGALLNLCMYQGNKGRAFRAGIVKLLLKMLSDSNGTLVDDALYIMSVLCSHPEAK 595

Query: 541 AAMANANTLLVLTDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLAEN 600
           AAM NAN+LLVLT+VLK GSSRS+ENA AVLLALCKGDWEKLEWLTRLGA   LMKL+E+
Sbjct: 596 AAMGNANSLLVLTNVLKKGSSRSRENAVAVLLALCKGDWEKLEWLTRLGAAVPLMKLSED 655

Query: 601 GTGRARRKAASLLEQLRKS 615
           GT RARRKAASLL+QLRKS
Sbjct: 656 GTERARRKAASLLDQLRKS 673

BLAST of Bhi12G000764 vs. ExPASy TrEMBL
Match: A0A6J1FID8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111445603 PE=4 SV=1)

HSP 1 Score: 924.5 bits (2388), Expect = 2.4e-265
Identity = 489/619 (79.00%), Postives = 537/619 (86.75%), Query Frame = 0

Query: 1   MSGRSIAGAGDVMKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSSL 60
           MSGRSI GAGDVMKKDCTDLIRRIALLIHLAEEITNFC G  + FEAL  D  +SSSS L
Sbjct: 61  MSGRSITGAGDVMKKDCTDLIRRIALLIHLAEEITNFCGGFRDKFEALT-DSIASSSSCL 120

Query: 61  DCLSEVVGAIQAAKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSN 120
           DCLSEVVGAIQAAKRLLY A+TFSVYD++    T TE  TKKLVLQF YVTTRLETALSN
Sbjct: 121 DCLSEVVGAIQAAKRLLYAAMTFSVYDDDEGSPTPTEGGTKKLVLQFHYVTTRLETALSN 180

Query: 121 LPYDHFCVSDEVQEQVDLVRAQLRRASNKYESMSNPAEKKLQTRGSVKWMINNDVKSISS 180
           LP+DHFCV+DEVQEQVDLVRAQL RAS  YESMS+P  KKL+  GSVK MI +DVK++SS
Sbjct: 181 LPFDHFCVADEVQEQVDLVRAQLMRASKNYESMSDPIHKKLEATGSVKGMIFHDVKTMSS 240

Query: 181 VDDGDVESQHHPRNCNYPTSFDS-----VNSCFDESSSVVHSDMEDVLASKSQDEVRKPI 240
           VDD D +SQH P N +    FDS      NSCF+E SS VHS+MEDVL+ KSQDEV +  
Sbjct: 241 VDDADPDSQHRPPNRDDLICFDSDELKNSNSCFNECSS-VHSEMEDVLSIKSQDEVGQLD 300

Query: 241 EVDIPENFLCPISFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPN 300
            ++IPENF CPIS ELM+DPVI STGQTYERSN+Q WIDRGN +CPKTQEQLQ+LILTPN
Sbjct: 301 VIEIPENFFCPISLELMIDPVIISTGQTYERSNIQKWIDRGNTSCPKTQEQLQSLILTPN 360

Query: 301 FLMRKLIAEWCEEHNVKLEEGLTSRKFKKYRSFEDDCRRTLPIKTLVRHLSFGSIQEQKI 360
           F MR LI+EWC EHNV LE+GLT+RK KKYRSFED CRR LPIKTLVRHLS GS+QEQK 
Sbjct: 361 FAMRNLISEWCGEHNVNLEKGLTNRKLKKYRSFEDGCRRMLPIKTLVRHLSLGSVQEQKA 420

Query: 361 AVTEIRKLSKSSSDHRVEIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLHEQNKRLV 420
           AVTEIR+LSKSSSDHRVEIA+AGAIPQLV LLTSED+ TQENA+SCILNLSLHE NKRL+
Sbjct: 421 AVTEIRQLSKSSSDHRVEIAKAGAIPQLVVLLTSEDVATQENAISCILNLSLHEPNKRLI 480

Query: 421 MLSGAVSYISKVLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSPR 480
           ML GA SYIS+VLK GSMEGRECAA TIYSLSLADENKAIIGASGVIPDL+EIL+IGSPR
Sbjct: 481 MLHGAFSYISQVLKFGSMEGRECAAGTIYSLSLADENKAIIGASGVIPDLIEILQIGSPR 540

Query: 481 GQKDAAGALLNLCMYQGNKGRALKAGIVRPLLKILSDPNGSLVDDALYIMSVLCGHPEAK 540
           GQKDAAGALLNLCMYQGNKGRA +AGI++ LLK+LSD NG+LVDDALYIMSVLC HPEAK
Sbjct: 541 GQKDAAGALLNLCMYQGNKGRAFRAGILKLLLKMLSDSNGTLVDDALYIMSVLCSHPEAK 600

Query: 541 AAMANANTLLVLTDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLAEN 600
           AAM NAN+LLVLT+VLK GSSRS+ENA AVLLALCKGDWEKLEWLTRLGA   LMKL+E+
Sbjct: 601 AAMGNANSLLVLTNVLKKGSSRSRENAVAVLLALCKGDWEKLEWLTRLGAAVPLMKLSED 660

Query: 601 GTGRARRKAASLLEQLRKS 615
           GT RA+RKAASLL+QLRKS
Sbjct: 661 GTERAKRKAASLLDQLRKS 677

BLAST of Bhi12G000764 vs. ExPASy TrEMBL
Match: A0A6J1FNY4 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111445603 PE=4 SV=1)

HSP 1 Score: 924.5 bits (2388), Expect = 2.4e-265
Identity = 489/619 (79.00%), Postives = 537/619 (86.75%), Query Frame = 0

Query: 1   MSGRSIAGAGDVMKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSSL 60
           MSGRSI GAGDVMKKDCTDLIRRIALLIHLAEEITNFC G  + FEAL  D  +SSSS L
Sbjct: 56  MSGRSITGAGDVMKKDCTDLIRRIALLIHLAEEITNFCGGFRDKFEALT-DSIASSSSCL 115

Query: 61  DCLSEVVGAIQAAKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSN 120
           DCLSEVVGAIQAAKRLLY A+TFSVYD++    T TE  TKKLVLQF YVTTRLETALSN
Sbjct: 116 DCLSEVVGAIQAAKRLLYAAMTFSVYDDDEGSPTPTEGGTKKLVLQFHYVTTRLETALSN 175

Query: 121 LPYDHFCVSDEVQEQVDLVRAQLRRASNKYESMSNPAEKKLQTRGSVKWMINNDVKSISS 180
           LP+DHFCV+DEVQEQVDLVRAQL RAS  YESMS+P  KKL+  GSVK MI +DVK++SS
Sbjct: 176 LPFDHFCVADEVQEQVDLVRAQLMRASKNYESMSDPIHKKLEATGSVKGMIFHDVKTMSS 235

Query: 181 VDDGDVESQHHPRNCNYPTSFDS-----VNSCFDESSSVVHSDMEDVLASKSQDEVRKPI 240
           VDD D +SQH P N +    FDS      NSCF+E SS VHS+MEDVL+ KSQDEV +  
Sbjct: 236 VDDADPDSQHRPPNRDDLICFDSDELKNSNSCFNECSS-VHSEMEDVLSIKSQDEVGQLD 295

Query: 241 EVDIPENFLCPISFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPN 300
            ++IPENF CPIS ELM+DPVI STGQTYERSN+Q WIDRGN +CPKTQEQLQ+LILTPN
Sbjct: 296 VIEIPENFFCPISLELMIDPVIISTGQTYERSNIQKWIDRGNTSCPKTQEQLQSLILTPN 355

Query: 301 FLMRKLIAEWCEEHNVKLEEGLTSRKFKKYRSFEDDCRRTLPIKTLVRHLSFGSIQEQKI 360
           F MR LI+EWC EHNV LE+GLT+RK KKYRSFED CRR LPIKTLVRHLS GS+QEQK 
Sbjct: 356 FAMRNLISEWCGEHNVNLEKGLTNRKLKKYRSFEDGCRRMLPIKTLVRHLSLGSVQEQKA 415

Query: 361 AVTEIRKLSKSSSDHRVEIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLHEQNKRLV 420
           AVTEIR+LSKSSSDHRVEIA+AGAIPQLV LLTSED+ TQENA+SCILNLSLHE NKRL+
Sbjct: 416 AVTEIRQLSKSSSDHRVEIAKAGAIPQLVVLLTSEDVATQENAISCILNLSLHEPNKRLI 475

Query: 421 MLSGAVSYISKVLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSPR 480
           ML GA SYIS+VLK GSMEGRECAA TIYSLSLADENKAIIGASGVIPDL+EIL+IGSPR
Sbjct: 476 MLHGAFSYISQVLKFGSMEGRECAAGTIYSLSLADENKAIIGASGVIPDLIEILQIGSPR 535

Query: 481 GQKDAAGALLNLCMYQGNKGRALKAGIVRPLLKILSDPNGSLVDDALYIMSVLCGHPEAK 540
           GQKDAAGALLNLCMYQGNKGRA +AGI++ LLK+LSD NG+LVDDALYIMSVLC HPEAK
Sbjct: 536 GQKDAAGALLNLCMYQGNKGRAFRAGILKLLLKMLSDSNGTLVDDALYIMSVLCSHPEAK 595

Query: 541 AAMANANTLLVLTDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLAEN 600
           AAM NAN+LLVLT+VLK GSSRS+ENA AVLLALCKGDWEKLEWLTRLGA   LMKL+E+
Sbjct: 596 AAMGNANSLLVLTNVLKKGSSRSRENAVAVLLALCKGDWEKLEWLTRLGAAVPLMKLSED 655

Query: 601 GTGRARRKAASLLEQLRKS 615
           GT RA+RKAASLL+QLRKS
Sbjct: 656 GTERAKRKAASLLDQLRKS 672

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G23030.16.2e-14148.28ARM repeat superfamily protein [more]
AT1G71020.11.9e-13748.31ARM repeat superfamily protein [more]
AT1G71020.23.9e-11150.42ARM repeat superfamily protein [more]
AT3G54850.16.2e-10940.52plant U-box 14 [more]
AT3G46510.17.1e-10537.60plant U-box 13 [more]
Match NameE-valueIdentityDescription
Q8GUG98.7e-14048.28U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2... [more]
Q9C9A62.6e-13648.31U-box domain-containing protein 10 OS=Arabidopsis thaliana OX=3702 GN=PUB10 PE=2... [more]
Q8VZ408.7e-10840.52U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1... [more]
Q5VRH94.8e-10640.74U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=P... [more]
Q9SNC61.0e-10337.60U-box domain-containing protein 13 OS=Arabidopsis thaliana OX=3702 GN=PUB13 PE=1... [more]
Match NameE-valueIdentityDescription
XP_038875664.10.0e+00100.00U-box domain-containing protein 11-like isoform X2 [Benincasa hispida][more]
XP_038875663.10.0e+00100.00U-box domain-containing protein 11-like isoform X1 [Benincasa hispida][more]
XP_038875665.10.0e+00100.00U-box domain-containing protein 11-like isoform X3 [Benincasa hispida][more]
XP_008467149.11.8e-29687.18PREDICTED: U-box domain-containing protein 11 [Cucumis melo][more]
KAE8648611.11.0e-29486.71hypothetical protein Csa_009171 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A1S3CSU48.9e-29787.18RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103504572 PE=4 ... [more]
A0A6J1K2B74.6e-26979.97RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111489474 P... [more]
A0A6J1JYM04.6e-26979.97RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111489474 P... [more]
A0A6J1FID82.4e-26579.00RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111445603... [more]
A0A6J1FNY42.4e-26579.00RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111445603... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 129..149
NoneNo IPR availablePANTHERPTHR23315:SF52U-BOX DOMAIN-CONTAINING PROTEIN 11coord: 11..613
NoneNo IPR availablePANTHERPTHR23315U BOX DOMAIN-CONTAININGcoord: 11..613
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 243..285
e-value: 4.21977E-22
score: 87.289
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 234..312
IPR000225ArmadilloSMARTSM00185arm_5coord: 449..489
e-value: 0.12
score: 21.4
coord: 367..407
e-value: 0.0034
score: 26.6
coord: 408..448
e-value: 96.0
score: 5.6
coord: 490..530
e-value: 180.0
score: 3.5
coord: 531..571
e-value: 23.0
score: 10.5
IPR000225ArmadilloPFAMPF00514Armcoord: 367..406
e-value: 6.3E-8
score: 32.4
coord: 450..488
e-value: 1.9E-4
score: 21.4
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 378..420
score: 12.6524
IPR003613U box domainSMARTSM00504Ubox_2coord: 242..305
e-value: 3.7E-24
score: 96.3
IPR003613U box domainPFAMPF04564U-boxcoord: 239..310
e-value: 7.9E-20
score: 70.8
IPR003613U box domainPROSITEPS51698U_BOXcoord: 238..312
score: 36.145298
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 220..320
e-value: 1.3E-26
score: 94.5
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 337..456
e-value: 1.5E-19
score: 71.8
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 457..614
e-value: 1.4E-24
score: 88.8
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 338..611

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi12M000764Bhi12M000764mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity