Bhi12G000657 (gene) Wax gourd (B227) v1

Overview


Name	Bhi12G000657
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Mannan endo-1,4-beta-mannosidase
Location	chr12: 22239948 .. 22244218 (-)
RNA-Seq Expression	Bhi12G000657
Synteny	Bhi12G000657

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTAAAGATTATTTATGATGATCCTCAATGCAGAAAGAGCCTGTTGAGTTTTTAAATTTCCCAACACAAAGTTGGCCAACAACCTCCTACATTAATTTCTCTTCTTGCATTATGACACCCAAAAAAAAGGCTTCAAGCACTATAAAAAATGGCTTGGAATAGCCCCATTTCTCCTTTGTAGAAATAAAAACCAATGGGAAACACTACTCAAATCAACTTTGTGTTTTTAGCTTTCATCTCCTTTTTCTCTCTTTCTTATTCTTTTCCTCTCTCAACCAATGGGAGATGGATTGTCGATTCCGCAGGACGTCGAATGAAGCTTATGTGCGTGAATTGGCCTTCTCACACTCAGAGCATGCTAATTGAAGGCCTTGATCGTAGGCCGTTGAAAGATCTTGCTGATGAGGTGGTGCGACTGAGGTTTAATTGTGTGAGACTTACATATGCAACTCACATGTTTACTCGATATGCTAATAGGACAGTTCAAGAGAATTTTGACCTTCTTGATTTGAAGGCGTCCAAGATGGGGTTAGCTTTGCATAATCCGTTCATATTGAACATGACTGTTTTCAATGCTTATGAAGCTGCGGTCGATATACTTGGGACAAGTGGTTTGATGGTTATAGCTGACAATCATATTAGTCAACCAAAATGGTGTTGCTCTCTTGACGACGGAAATGGTTTTTTTGGAGATCGCTATTTCGACCCAGAAGAATGGTTGGAAGGTCTTCGTTTGGTTGCCCGACGATTCTACAACAAATCAAATGTACGTAAGTGTTGTACTTAATTTTGAAATGATTTTTTTCGAAATGTGAGAGTATAACGTAGAGATGCACATTTTATATAAGTAATTACAATTATAGTCCATTTATTAAATAAAAGATCAAATCCATATATTTATTAAAATAACCAAAGTAATCAAGCAAATTATAGTAACTTTTTTTTAGTGGTTATTATTTTAGTAATATTAGGCTATTATTATAATGGTTAAATTACATTTTAAATCCCTTAATACTTTAAAATATTAATTTCAATTTTGTCTCTAATTTTTTCAGAAGTGTCATTTACCTTGAAAGACATGGCAAGTTGGTTACCATCCTCGGTACAACCATTTATTTTAAGGAAAGAGTTCGACAAGTCATATTGGAAAAAAAATACACATAAATGTGGTATATCTTAAAACGGGGGAAAATATCAAGTTTGACATTAAACTTCTCAAAGTTATATCAATTTTTATCCTATACTTTTACTTTCATCAAATTACACCCCAAACTTAAACGATTGTTGCAGTCTTAACCTTAAGCTTTAAAAAGAAAATTCTAATTTTAACCCTTATGCTCCAAAAATCATTATTAAAAAAAATTTGTTTGTGTTCTAGGAAATTGATAAAAATATATGTATGGTATAAAATTAATAGTTTTTTTTTTTTTAAATGATAAATTTGTCATTGAAATTTATAAAATTTTGGTAAAGTTGGTTCATAGTCAATTTGTACATTGGTCAAACTTATTTTTGTGCAAAATATTATTTTATTGAAATTGATGAGTTTAGGCCGAATATTTTAGAACATTTTCACAAAAATTTAGTGGGTGAATTAATGAAAATTGAACCCGTGGTAAAATTGCAACACTTATAAGTTTTTTGGTCTAATTTGATGATATTGAAAGTTTATGGTCTAACCGTTTATAGTCAACATTGATTTTCTCCTATAAAACTATAATATTTGAAAAGTATACCAAAGTCGAGATTATTTCAATTTTGGAATGTCAACCAATAATTTCATTTGCTTTTTAAGCTAATAAAAAAGACTCATCAAAGTAGGATTTTCATTATATCTAACTAAAATCTTGAGGTTTTATTTTTGAAACTAAGCTCAGAATTAAAATATATTTTAAAGAATGTAAAAGATTCACTTCAAAATTGTAAAGAAGCCAACATAATTTTCAAAAATAAAATAATTATCAAATGGAGTCCTAATATCTATAATGAAAATAATTAATAAATAAAATGTCAACACGTAAAATTTTGTCCACCCACTTTAAGAAAATTATTTACTTTACCCTTGTATCAAACAGGTGGTAGCTATGAGCCTACGAAACGAACTCCGAGGCACAAAGTCAAAATCAAAAGATTGGAACAAATACATGACACAAGGAGCAACAACAATCCACACCATAAATCCGAACATCCTCATAATCATTTCAGGTCTAAACTTCGACAACGACCTAAGATGCCAAAGACAAAACCCCTTGCCACTAAACAACCTACATAACAAGCTAGTTTTTGAAGTACACTTATATTCCTTCAGTGGAGAATCCCAATCAAAGTTCATTAATAATCCTCTCAACAAAATATGCTCAAAGATCATCAATGGGTTTGTGGAGAGAGCTGAGTTTGTGATGGAAGGAGCTGAGGCAGTTCCTTTGTTTGTGAGTGAGTTTGGGTTTGATCAAAGGGGAGTTAATGAGGCTGATGATAGGTTTTTGAGTTGCTTTAGGGCTCATCTTGCTAAGAAAGATTTGGATTGGGCATTATGGGCTTGGCAAGGGAGTTATTATTATAGACAGGGCAAGATCGAACCAGAAGAAGTGTTTGGAGTTTTGAACTATAATTGGACGGATGTTAGAAACCCTCATTTTTCTCAAATGTTTCAGCTCTTGCAAACCATGTTACAAGGTATGACTTCGAGTACTGTTTGACGGTTCACCTTTAAAACAAAGATTAAAATTTTATTATTTTGTGTAAATAATTTTCTTTATTTTTCTATTTTTAAAAATTCCCAATTGCTTTTTCAATTTCATTGATTTCTAAAATCTGCCTAGAGTAGCTTTTAGGATAAACTTCCTAAAGAAGCTTCATACCTCTTATAGCTAACTATTAAAATTCCTATTTATCTGTATTCTACAATATTTTTTGTTATTTAGATTTGAAATTTACCGAACACTTATTTACTTAATTTTACATATAATTTGTTTAAAATTTCAGTTACTAATAATTATTTTAAAAGTTTCTTCAATCTCAAATCAATTCCATTTCTTGACAATGAAACTTGATGATAGAAACTTCGCTACATAAATATGGACACGGGACAATCTTAAATTAAAATATGTGCCATTTGTATTAAAAAAAACCTACCATTTATGACAGCCTCTTCTCTACAAGTTGATACTTTTTTCTTTTAGATATTTATGAGTATTTGACCTACTTATGTATATTTTGATTATCTCTGAAACAACTCACTCTATCCTATAAAACATTCATACGATATTAAATCATAGGTTGATTGGCCATCGTAGATCAAACTCATTCTCTCTAATTTTTTTTTTTCTAATTAAATTAAATCACCCATGACTTTTATTGAGCATTAAGTCAATCTTTGATGGTTTACAAATTAATGCATACGTGTAGATTTAGTCGTGTGATTTAACATGATATCCAAACAAAAAAATTTTTTTTTGTTTGAATTGAGATAAAAGTTATTTCTTCCCTGAATCAAATTTATATTAGAAATTAACAACATTAAGCTCAATTTTATGGTTTGAAGGTGGTTGAAATAGAAATTAGGTTAAAGTATTATTCCAATTCCACAAGGATACATTTTGTATAGACCCTTTTATGACAAATTACATCTATAATTTTAAAAATTATTAGTATGAAAATTGAAAACTATATGTATAATAGTCCTTTTTTTCCCCCTTTTTCTTCATTTTTTCCCTTTATTGGTAACTTAAACTTTATGCACATGGCAGATCCAAATTCCAATTCCTCAAACACTTATGTAATGTACCATCCACAAAGTGGGCAATGTGTCCAAGTAAAAGACATGATGGATAAGCAAATTTATCTCAACAACTGTTCCAATGCAAGCCATTGGAGTCATGAAGGAGATGGGACTCCAATAATGTTGGAAGCCACTAATTTTTGTCTAAAAGCCAATGGAAATGGGCTTCCACCATCACTTTCAAGTGATTGTTTTGGTGAGCAAAGTGTTTGGACAGCCATTTCAGACTCTAAGCTTCATTTGGCAACACTAACAAAACAAGGGAATAATGGTTTGTGTTTAGAGAAAGAGAGCTCAAATTCAACTAGGATTGTGATGGGGAGTTGTGTTTGTGTTGGTAATGATTCTAATTGTTTGCAAGATACTCAAGCTCAATGGTTTGAACTTGTTGTTACAAATACTTGGTAATTTTTTACATGGAGAGAAAATGATGAAATAAGAAAGAAATCATTTAGACTCAATTATTGTTCAATGAGTTCTACTTTGTGTCA

mRNA sequence

CTAAAGATTATTTATGATGATCCTCAATGCAGAAAGAGCCTGTTGAGTTTTTAAATTTCCCAACACAAAGTTGGCCAACAACCTCCTACATTAATTTCTCTTCTTGCATTATGACACCCAAAAAAAAGGCTTCAAGCACTATAAAAAATGGCTTGGAATAGCCCCATTTCTCCTTTGTAGAAATAAAAACCAATGGGAAACACTACTCAAATCAACTTTGTGTTTTTAGCTTTCATCTCCTTTTTCTCTCTTTCTTATTCTTTTCCTCTCTCAACCAATGGGAGATGGATTGTCGATTCCGCAGGACGTCGAATGAAGCTTATGTGCGTGAATTGGCCTTCTCACACTCAGAGCATGCTAATTGAAGGCCTTGATCGTAGGCCGTTGAAAGATCTTGCTGATGAGGTGGTGCGACTGAGGTTTAATTGTGTGAGACTTACATATGCAACTCACATGTTTACTCGATATGCTAATAGGACAGTTCAAGAGAATTTTGACCTTCTTGATTTGAAGGCGTCCAAGATGGGGTTAGCTTTGCATAATCCGTTCATATTGAACATGACTGTTTTCAATGCTTATGAAGCTGCGGTCGATATACTTGGGACAAGTGGTTTGATGGTTATAGCTGACAATCATATTAGTCAACCAAAATGGTGTTGCTCTCTTGACGACGGAAATGGTTTTTTTGGAGATCGCTATTTCGACCCAGAAGAATGGTTGGAAGGTCTTCGTTTGGTTGCCCGACGATTCTACAACAAATCAAATGTGGTAGCTATGAGCCTACGAAACGAACTCCGAGGCACAAAGTCAAAATCAAAAGATTGGAACAAATACATGACACAAGGAGCAACAACAATCCACACCATAAATCCGAACATCCTCATAATCATTTCAGGTCTAAACTTCGACAACGACCTAAGATGCCAAAGACAAAACCCCTTGCCACTAAACAACCTACATAACAAGCTAGTTTTTGAAGTACACTTATATTCCTTCAGTGGAGAATCCCAATCAAAGTTCATTAATAATCCTCTCAACAAAATATGCTCAAAGATCATCAATGGGTTTGTGGAGAGAGCTGAGTTTGTGATGGAAGGAGCTGAGGCAGTTCCTTTGTTTGTGAGTGAGTTTGGGTTTGATCAAAGGGGAGTTAATGAGGCTGATGATAGGTTTTTGAGTTGCTTTAGGGCTCATCTTGCTAAGAAAGATTTGGATTGGGCATTATGGGCTTGGCAAGGGAGTTATTATTATAGACAGGGCAAGATCGAACCAGAAGAAGTGTTTGGAGTTTTGAACTATAATTGGACGGATGTTAGAAACCCTCATTTTTCTCAAATGTTTCAGCTCTTGCAAACCATGTTACAAGATCCAAATTCCAATTCCTCAAACACTTATGTAATGTACCATCCACAAAGTGGGCAATGTGTCCAAGTAAAAGACATGATGGATAAGCAAATTTATCTCAACAACTGTTCCAATGCAAGCCATTGGAGTCATGAAGGAGATGGGACTCCAATAATGTTGGAAGCCACTAATTTTTGTCTAAAAGCCAATGGAAATGGGCTTCCACCATCACTTTCAAGTGATTGTTTTGGTGAGCAAAGTGTTTGGACAGCCATTTCAGACTCTAAGCTTCATTTGGCAACACTAACAAAACAAGGGAATAATGGTTTGTGTTTAGAGAAAGAGAGCTCAAATTCAACTAGGATTGTGATGGGGAGTTGTGTTTGTGTTGGTAATGATTCTAATTGTTTGCAAGATACTCAAGCTCAATGGTTTGAACTTGTTGTTACAAATACTTGGTAATTTTTTACATGGAGAGAAAATGATGAAATAAGAAAGAAATCATTTAGACTCAATTATTGTTCAATGAGTTCTACTTTGTGTCA

Coding sequence (CDS)

ATGGGAAACACTACTCAAATCAACTTTGTGTTTTTAGCTTTCATCTCCTTTTTCTCTCTTTCTTATTCTTTTCCTCTCTCAACCAATGGGAGATGGATTGTCGATTCCGCAGGACGTCGAATGAAGCTTATGTGCGTGAATTGGCCTTCTCACACTCAGAGCATGCTAATTGAAGGCCTTGATCGTAGGCCGTTGAAAGATCTTGCTGATGAGGTGGTGCGACTGAGGTTTAATTGTGTGAGACTTACATATGCAACTCACATGTTTACTCGATATGCTAATAGGACAGTTCAAGAGAATTTTGACCTTCTTGATTTGAAGGCGTCCAAGATGGGGTTAGCTTTGCATAATCCGTTCATATTGAACATGACTGTTTTCAATGCTTATGAAGCTGCGGTCGATATACTTGGGACAAGTGGTTTGATGGTTATAGCTGACAATCATATTAGTCAACCAAAATGGTGTTGCTCTCTTGACGACGGAAATGGTTTTTTTGGAGATCGCTATTTCGACCCAGAAGAATGGTTGGAAGGTCTTCGTTTGGTTGCCCGACGATTCTACAACAAATCAAATGTGGTAGCTATGAGCCTACGAAACGAACTCCGAGGCACAAAGTCAAAATCAAAAGATTGGAACAAATACATGACACAAGGAGCAACAACAATCCACACCATAAATCCGAACATCCTCATAATCATTTCAGGTCTAAACTTCGACAACGACCTAAGATGCCAAAGACAAAACCCCTTGCCACTAAACAACCTACATAACAAGCTAGTTTTTGAAGTACACTTATATTCCTTCAGTGGAGAATCCCAATCAAAGTTCATTAATAATCCTCTCAACAAAATATGCTCAAAGATCATCAATGGGTTTGTGGAGAGAGCTGAGTTTGTGATGGAAGGAGCTGAGGCAGTTCCTTTGTTTGTGAGTGAGTTTGGGTTTGATCAAAGGGGAGTTAATGAGGCTGATGATAGGTTTTTGAGTTGCTTTAGGGCTCATCTTGCTAAGAAAGATTTGGATTGGGCATTATGGGCTTGGCAAGGGAGTTATTATTATAGACAGGGCAAGATCGAACCAGAAGAAGTGTTTGGAGTTTTGAACTATAATTGGACGGATGTTAGAAACCCTCATTTTTCTCAAATGTTTCAGCTCTTGCAAACCATGTTACAAGATCCAAATTCCAATTCCTCAAACACTTATGTAATGTACCATCCACAAAGTGGGCAATGTGTCCAAGTAAAAGACATGATGGATAAGCAAATTTATCTCAACAACTGTTCCAATGCAAGCCATTGGAGTCATGAAGGAGATGGGACTCCAATAATGTTGGAAGCCACTAATTTTTGTCTAAAAGCCAATGGAAATGGGCTTCCACCATCACTTTCAAGTGATTGTTTTGGTGAGCAAAGTGTTTGGACAGCCATTTCAGACTCTAAGCTTCATTTGGCAACACTAACAAAACAAGGGAATAATGGTTTGTGTTTAGAGAAAGAGAGCTCAAATTCAACTAGGATTGTGATGGGGAGTTGTGTTTGTGTTGGTAATGATTCTAATTGTTTGCAAGATACTCAAGCTCAATGGTTTGAACTTGTTGTTACAAATACTTGGTAA

Protein sequence

MGNTTQINFVFLAFISFFSLSYSFPLSTNGRWIVDSAGRRMKLMCVNWPSHTQSMLIEGLDRRPLKDLADEVVRLRFNCVRLTYATHMFTRYANRTVQENFDLLDLKASKMGLALHNPFILNMTVFNAYEAAVDILGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEGLRLVARRFYNKSNVVAMSLRNELRGTKSKSKDWNKYMTQGATTIHTINPNILIIISGLNFDNDLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFINNPLNKICSKIINGFVERAEFVMEGAEAVPLFVSEFGFDQRGVNEADDRFLSCFRAHLAKKDLDWALWAWQGSYYYRQGKIEPEEVFGVLNYNWTDVRNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVQVKDMMDKQIYLNNCSNASHWSHEGDGTPIMLEATNFCLKANGNGLPPSLSSDCFGEQSVWTAISDSKLHLATLTKQGNNGLCLEKESSNSTRIVMGSCVCVGNDSNCLQDTQAQWFELVVTNTW

Homology

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BLAST of Bhi12G000657 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 425.2 bits (1092), Expect = 7.6e-119
Identity = 219/536 (40.86%), Postives = 325/536 (60.63%), Query Frame = 0


Query: 9   FVFLAFISFFSL-------SYSFPLSTNGRWIVDSAGRRMKLMCVNWPSHTQSMLIEGLD 68
           F+F  F  FFS        + S+PLST+ RWIVD  G R+KL+C NWPSH Q ++ EGL 
Sbjct: 11  FIFPFFCFFFSFIAQNTVPNMSYPLSTSSRWIVDENGLRVKLVCANWPSHLQPVVAEGLS 70

Query: 69  RRPLKDLADEVVRLRFNCVRLTYATHMFTRYA---NRTVQENFDLLDLKASKMGLALHNP 128
           ++P+  +A ++V + FNCVRLT+   + T      N TV+++F  L L    +G   +NP
Sbjct: 71  KQPVDAVAKKIVEMGFNCVRLTWPLDLMTNETLANNVTVRQSFQSLGLNDDIVGFQTNNP 130

Query: 129 FILNMTVFNAYEAAVDILGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEG 188
            I+++ +  AY+  V  LG + +MVI DNH+++P WCC+ DDGNGFFGD++FDP  W+  
Sbjct: 131 SIIDLPLIEAYKTVVTTLGNNDVMVILDNHLTKPGWCCANDDGNGFFGDQFFDPTVWVAA 190

Query: 189 LRLVARRFYNKSNVVAMSLRNELRGTKSKSKDWNKYMTQGATTIHTINPNILIIISGLNF 248
           L+ +A  F   SNVV MSLRNELRG K    DW KYM QGA  +H+ N  +L+I+SGL+F
Sbjct: 191 LKKMAATFNGVSNVVGMSLRNELRGPKQNVNDWFKYMQQGAEAVHSANNKVLVILSGLSF 250

Query: 249 DNDLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFINNPLNKICSKIINGFVERAEF 308
           D DL   R  P+ L +   KLVFE+H YSFS +  S   NNP N IC +++N       +
Sbjct: 251 DADLSFVRSRPVKL-SFTGKLVFELHWYSFS-DGNSWAANNP-NDICGRVLNRIGNGGGY 310

Query: 309 VMEGAEAVPLFVSEFGFDQRGVNEADDRFLSCFRAHLAKKDLDWALWAWQGSYYYRQGKI 368
           ++   +  PLF+SEFG D+RGVN  D+R+  C     A+ D+DW+LWA  GSYY RQGK+
Sbjct: 311 LLN--QGFPLFLSEFGIDERGVNTNDNRYFGCLTGWAAENDVDWSLWALTGSYYLRQGKV 370

Query: 369 EPEEVFGVLNYNWTDVRNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVQVKDMM 428
              E +GVL+ +W  VRN  F Q    LQ+ LQ P   +    +++HP +G C+ V+ + 
Sbjct: 371 GMNEYYGVLDSDWISVRNSSFLQKISFLQSPLQGPGPRTDAYNLVFHPLTGLCI-VRSLD 430

Query: 429 D-KQIYLNNCSNASHWSHEGDGTPIMLEATNFCLKANGNGLPPSLS-SDCFGEQSVWTAI 488
           D K + L  C+++  WS+      + ++    CL++NG   P +++ + C    S W  I
Sbjct: 431 DPKMLTLGPCNSSEPWSYTKKA--LRIKDQQLCLQSNGPKNPVTMTRTSCSTSGSKWQTI 490

Query: 489 SDSKLHLATLTKQGNNGLCLEKESSNSTRIVMGSCVCVGNDSNCLQDTQAQWFELV 533
           S S++HLA+ T      LCL+ +++N+  +V  +C C+  D +C  +  +QWF+++
Sbjct: 491 SASRMHLAS-TTSNKTSLCLDVDTANN--VVANACKCLSKDKSC--EPMSQWFKII 533

[Close]

BLAST of Bhi12G000657 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 414.8 bits (1065), Expect = 1.0e-115
Identity = 225/534 (42.13%), Postives = 323/534 (60.49%), Query Frame = 0


Query: 14  FISFFSLSY-----SFPLSTNGRWIVD--SAGRRMKLMCVNWPSHTQSMLIEGLDRRPLK 73
           FIS F L Y     +FP ST+ RWIVD  + GRR+KL CVNWPSH ++ + EGL ++PL 
Sbjct: 6   FISVFLLPYVITTFAFPPSTDSRWIVDDGNKGRRVKLTCVNWPSHLETAVAEGLSKQPLD 65

Query: 74  DLADEVVRLRFNCVRLTYATHMFTR---YANRTVQENFDLLDLKASKMGLALHNPFILNM 133
            +A+++V + FNCVRLT+  ++ T     A  TV+++     L  +  G   HNP IL++
Sbjct: 66  AIAEKIVSMGFNCVRLTWPLYLATDESFSAFMTVRQSLRKFRLFEAVSGFQTHNPTILDL 125

Query: 134 TVFNAYEAAVDILGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEGLRLVA 193
            +  A++  V  L    +MVI DNHISQP WCCS +DGNGFFGD++ +P+ W++GL+ +A
Sbjct: 126 PLIKAFQEVVYCLEKHRVMVILDNHISQPGWCCSDNDGNGFFGDKHLNPQVWIKGLKKMA 185

Query: 194 RRFYN-KSNVVAMSLRNELRGTKSKSKDWNKYMTQGATTIHTINPNILIIISGLNFDNDL 253
             F N  SNVV MSLRNELRG K   KDW KYM +GA  +H++NPN+L+I+SGLN+  DL
Sbjct: 186 SMFANVSSNVVGMSLRNELRGPKQNIKDWYKYMREGAEAVHSVNPNVLVIVSGLNYATDL 245

Query: 254 RCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFINNPLNKICSKIINGFVERAEFVMEG 313
              R+ P  + +   K+VFE+H Y F     + +  + LNKIC K     ++ + F++E 
Sbjct: 246 SFLRERPFEV-SFRRKVVFEIHWYGF----WNTWEGDNLNKICGKETEKMMKMSGFLLE- 305

Query: 314 AEAVPLFVSEFGFDQRGVNEADDRFLSCFRAHLAKKDLDWALWAWQGSYYYRQGKIEPEE 373
            + +PLFVSEFG DQRG N  D++FLSCF A  A +DLDW+LW   GSYY R+  I  +E
Sbjct: 306 -KGIPLFVSEFGIDQRGNNANDNKFLSCFMALAADRDLDWSLWTLAGSYYIREKSIGSDE 365

Query: 374 VFGVLNYNWTDVRNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVQVKDMMDKQI 433
            +GVL++NW+ +RN    QM   +QT             +M+HP +G C+  K +   Q+
Sbjct: 366 SYGVLDFNWSSIRNSTILQMISAIQTPFIGLMETQPKK-IMFHPSTGLCIVRKSLF--QL 425

Query: 434 YLNNCSNASHWSHEGDGTPIMLEATNFCLKANGNGLPPSLSSDCFGEQ--SVWTAISDSK 493
            L +C+ +  W         + E    CLKA   G    L    F E   S W   SDSK
Sbjct: 426 KLGSCNRSESWRLSSHRVLSLAEEQILCLKAYEKGKSVKLRL-FFSESYCSKWKLFSDSK 485

Query: 494 LHLATLTKQGNNGLCLEKESSNSTRIVMGSCVCVGNDSNCLQDTQAQWFELVVT 535
           + L+++TK G + +CL+ ++ N+  IV  SC C+  +S+C  D ++QWF+LV +
Sbjct: 486 MQLSSITKNGFS-VCLDVDTENN-NIVTNSCKCLRGNSSC--DPRSQWFKLVTS 524

[Close]

BLAST of Bhi12G000657 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 396.7 bits (1018), Expect = 2.9e-110
Identity = 206/516 (39.92%), Postives = 303/516 (58.72%), Query Frame = 0


Query: 23  SFPLSTNGRWIVDSAGRRMKLMCVNWPSHTQSMLIEGLDRRPLKDLADEVVRLRFNCVRL 82
           ++PLSTN RWI+D  G+R+KL CVNWPSH Q ++ EGL ++ + DLA +++ + FNCVR 
Sbjct: 2   AYPLSTNSRWIIDEKGQRVKLACVNWPSHLQPVVAEGLSKQSVDDLAKKIMAMGFNCVRF 61

Query: 83  TYATHMFTRYA---NRTVQENFDLLDLKASKMGLALHNPFILNMTVFNAYEAAVDILGTS 142
           T+   + T      N TV+++F  L L     G    NP ++++ +  AY+  V  LG +
Sbjct: 62  TWPLDLATNETLANNVTVRQSFQSLGLNDDISGFETKNPSMIDLPLIEAYKKVVAKLGNN 121

Query: 143 GLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEGLRLVARRFYNKSNVVAMSLRN 202
            +MVI DNH+++P WCC  +DGNGFFGD +FDP  W+ GL  +A  F   +NVV MSLRN
Sbjct: 122 NVMVILDNHVTKPGWCCGYNDGNGFFGDTFFDPTTWIAGLTKIAMTFKGATNVVGMSLRN 181

Query: 203 ELRGTKSKSKDWNKYMTQGATTIHTINPNILIIISGLNFDNDLRCQRQNPLPLNNLHNKL 262
           ELRG K    DW KYM QGA  +H  NPN+L+I+SGL++D DL   R   + L     KL
Sbjct: 182 ELRGPKQNVDDWFKYMQQGAEAVHEANPNVLVILSGLSYDTDLSFVRSRHVNL-TFTRKL 241

Query: 263 VFEVHLYSFSGESQSKFINNPLNKICSKIINGFVERAEFVMEGAEAVPLFVSEFGFDQRG 322
           VFE+H YSF+  + +    NP N+ C +I+        F +      P+F+SEFG D RG
Sbjct: 242 VFELHRYSFT-NTNTWSSKNP-NEACGEILKSIENGGGFNLRD---FPVFLSEFGIDLRG 301

Query: 323 VNEADDRFLSCFRAHLAKKDLDWALWAWQGSYYYRQGKIEPEEVFGVLNYNWTDVRNPHF 382
            N  D+R++ C     A+ D+DW++W  QGSYY R+G +   E +G+L+ +W  VR+  F
Sbjct: 302 KNVNDNRYIGCILGWAAENDVDWSIWTLQGSYYLREGVVGMSEFYGILDSDWVRVRSQSF 361

Query: 383 SQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVQVKDMMDKQIYLNNCSNASHWSHEGDG 442
            Q   L+ + LQ P S S    +++HP +G C+    +   ++ L  C+ +  WS+    
Sbjct: 362 LQRLSLILSPLQGPGSQSKVYNLVFHPLTGLCMLQSILDPTKVTLGLCNESQPWSYTPQN 421

Query: 443 TPIMLEATNFCLKANGNGLPPSLS-SDCFGEQ-SVWTAISDSKLHLATLTKQGNNGLCLE 502
           T + L+  + CL++ G   P  LS + C     S W  IS S + LA   K  NN LCL+
Sbjct: 422 T-LTLKDKSLCLESTGPNAPVKLSETSCSSPNLSEWETISASNMLLA--AKSTNNSLCLD 481

Query: 503 KESSNSTRIVMGSCVCV-GNDSNCLQDTQAQWFELV 533
            + +N+  ++  +C CV G DS+C  D  +QWF++V
Sbjct: 482 VDETNN--LMASNCKCVKGEDSSC--DPISQWFKIV 504

[Close]

BLAST of Bhi12G000657 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 368.6 bits (945), Expect = 8.4e-102
Identity = 200/528 (37.88%), Postives = 307/528 (58.14%), Query Frame = 0


Query: 9   FVFLAFISFFSLSYSFPLSTNGRWIVDSAGRRMKLMCVNWPSHTQSMLIEGLDRRPLKDL 68
           F+FL+ IS  +L+  +PL T  RWIV++ G R+KL C NWPSH + ++ EGL  +P+  +
Sbjct: 12  FLFLSLIS-LTLATDYPLFTKSRWIVNNKGHRVKLACANWPSHLKPVVAEGLSSQPMDSI 71

Query: 69  ADEVVRLRFNCVRLTYATHMF---TRYANRTVQENFDLLDLKASKMGLALHNPFILNMTV 128
           + ++  + FNCVRLT+   +    T   N TV+++F+   L     G+  HNP+I+N  +
Sbjct: 72  SKKIKDMGFNCVRLTWPLELMINDTLAFNVTVKQSFERYGLDHELQGIYTHNPYIVNTPL 131

Query: 129 FNAYEAAVDILGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEGLRLVARR 188
            N ++A V  LG   +MVI DNH + P WCCS DD + FFGD  F+P+ W+ GL+ +A  
Sbjct: 132 INVFQAVVYSLGRHDVMVILDNHKTVPGWCCSNDDPDAFFGDPKFNPDLWMLGLKKMATI 191

Query: 189 FYNKSNVVAMSLRNELRGTKSKSKDWNKYMTQGATTIHTINPNILIIISGLNFDNDLRCQ 248
           F N  NVV MSLRNELRG    SKDW KYM +GA  +HT NPN+L+I+SGLNFD DL   
Sbjct: 192 FMNVKNVVGMSLRNELRGYNHTSKDWYKYMQKGAEAVHTSNPNVLVILSGLNFDADLSFL 251

Query: 249 RQNPLPLNNLHNKLVFEVHLYSFSGESQSKFINNPLNKICSKIINGFVERAEFVMEGAEA 308
           +  P+ L +   KLV E+H YSF+ +   ++ ++ +N  CS++ +       FV++  + 
Sbjct: 252 KDRPVNL-SFKKKLVLELHWYSFT-DGTGQWKSHNVNDFCSQMFSKERRTGGFVLD--QG 311

Query: 309 VPLFVSEFGFDQRGVNEADDRFLSCFRAHLAKKDLDWALWAWQGSYYYRQGKIEPEEVFG 368
            PLF+SEFG DQRG +   +R+++C  A  A+KDLDWA+WA  G YY+R+GK    E +G
Sbjct: 312 FPLFLSEFGTDQRGGDLEGNRYMNCMLAWAAEKDLDWAVWAVTGVYYFREGKRGVVEAYG 371

Query: 369 VLNYNWTDVRNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQC-VQVKDMMDKQIYL 428
           +L+ NW +V N  + +   ++Q     P    ++   ++HP +G C V+     + ++ L
Sbjct: 372 MLDANWHNVHNYTYLRRLSVIQPPHTGPGVKHNHHKKIFHPLTGLCLVRKSHCHESELTL 431

Query: 429 NNCSNASHWSHEGDGTPIMLEATNFCLKA-NGNGLPPSLSSDCFGEQSVWTAISDSKLHL 488
             C+    WS+   G   +      CL+     G    L   C   +     IS +K+HL
Sbjct: 432 GPCTKDEPWSYSHGGILEIRRGHKSCLEGETAVGKSVKLGRICTKIEQ----ISATKMHL 491

Query: 489 ATLTKQGNNGLCLEKESSNSTRIVMGSCVCVGNDSNCLQDTQAQWFEL 532
           +  T  G+  +CL+ +S N+  +V  SC C+  D+ C  +  +QWF++
Sbjct: 492 SFNTSDGSL-VCLDVDSDNN--VVANSCNCLTGDTTC--EPASQWFKI 525

[Close]

BLAST of Bhi12G000657 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 307.8 bits (787), Expect = 1.8e-83
Identity = 186/537 (34.64%), Postives = 289/537 (53.82%), Query Frame = 0


Query: 8   NFVFLAFISFFS----LSYSFPLSTNGRWIVDSAGRRMKLMCVNWPSHTQSMLIEGLDRR 67
           +F F  F  F S    L+ S+PLST  RWIVD  G+R+KL CVNWP+H Q  + EGL ++
Sbjct: 4   SFYFCLFFLFISSTSKLTTSYPLSTKSRWIVDEKGQRVKLACVNWPAHLQPTVAEGLSKQ 63

Query: 68  PLKDLADEVVRLRFNCVRLTYATHMFTR---YANRTVQENFDLLDLKASKMGLALHNPFI 127
           PL  ++ ++V + FNCVRLT+   + T        TV+++F+ L L    +G+  HNP +
Sbjct: 64  PLDSISKKIVSMGFNCVRLTWPLDLVTNDTLALKVTVKQSFESLKLFEDVLGIQTHNPKL 123

Query: 128 LNMTVFNAYEAAVDILGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEGLR 187
           L++ +FNA++  V  LG +G+MVI DNH++ P WCC  +D + FFG  +FDP  W +GLR
Sbjct: 124 LHLPLFNAFQEVVSNLGENGVMVILDNHLTTPGWCCGDNDLDAFFGYPHFDPLVWAKGLR 183

Query: 188 LVARRFYNKSNVVAMSLRNELRGTKSKSKDWNKYMTQGATTIHTINPNILIIISGLNFDN 247
            +A  F N ++V+ MSLRNE RG +     W ++M QGA  +H  NP +L+I+SG++FD 
Sbjct: 184 KMATLFRNFTHVIGMSLRNEPRGARDYPDLWFRHMPQGAEAVHAANPKLLVILSGIDFDT 243

Query: 248 DLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFINNPLNKICSKIINGFVERAEFVM 307
           +L   R   + + +  +KLVFE+H YSFS + +  +  +  N  C KII        F++
Sbjct: 244 NLSFLRDRSVNV-SFTDKLVFELHWYSFS-DGRDSWRKHNSNDFCVKIIEKVTHNGGFLL 303

Query: 308 EGAEAVPLFVSEFGFDQRGVNEADDRFLSCFRAHLAKKDLDWALWAWQGSYYYRQGKIEP 367
                 PL +SEFG DQRG + + +R+++C  A  A+ DLDWA+WA  G YY R G    
Sbjct: 304 --GRGFPLILSEFGTDQRGGDMSGNRYMNCLVAWAAENDLDWAVWALTGDYYLRTGP--- 363

Query: 368 EEVFGVLNYNWTDVRNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVQVKDMMD- 427
               G+                          PN N     +++HP +G CV      + 
Sbjct: 364 ----GL-------------------------RPNKN-----LLFHPSTGLCVTNNPSDNI 423

Query: 428 KQIYLNNCSNASHWS-HEGDGTPIMLEATNFCLKAN---GNGLPPSLSSDCFGEQSVWTA 487
             + L  C  +  W+ +  +G   +L     C++A    G  +   + + C    S    
Sbjct: 424 PTLRLGPCPKSDPWTFNPSEG---ILWINKMCVEAPNVVGQKVKLGVGTKC----SKLGQ 483

Query: 488 ISDSKLHLATLTKQGNNGLCLEKESSNSTRIVMGSCVCVGNDSNCLQDTQAQWFELV 533
           IS +K+HL+  T  G   LCL+ +  +++ +V   C  +  D++C  D  +QWF+++
Sbjct: 484 ISATKMHLSFKTSNGLL-LCLDVDERDNS-VVANRCKFLTMDASC--DPASQWFKVL 488

[Close]

BLAST of Bhi12G000657 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 1.3e-115
Identity = 229/533 (42.96%), Postives = 319/533 (59.85%), Query Frame = 0


Query: 21  SYSFPLSTNGRWIVDSA-GRRMKLMCVNWPSHTQSMLIEGLDRRPLKDLADEVVRLRFNC 80
           S+S PL T GRWIVD A G R+KL CVNW  H +  L EGL+R P+  +A  +  L FNC
Sbjct: 26  SHSLPLLTRGRWIVDEATGLRVKLACVNWVGHLEPGLPEGLNRLPVATVAHTISSLGFNC 85

Query: 81  VRLTYATHMFTR--YANRTVQENFDLLDLKASKMGLALHNPFILNMTVFNAYEAAVDILG 140
           VRLTY+ HM TR  Y N TV + F  L+L  +  G+  +NP +L++    AY   V  L 
Sbjct: 86  VRLTYSIHMLTRTSYTNATVAQTFARLNLTEAASGIEHNNPELLDLGHVAAYHHVVAALS 145

Query: 141 TSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEGLRLVARRFYNKSNVVAMSL 200
            +G+MVI DNH+S+PKWCC++DDGNGFFGDRYF+P  W+EGL L+A  F N  NVVAMSL
Sbjct: 146 EAGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPNTWVEGLGLMATYFNNTPNVVAMSL 205

Query: 201 RNELRGTKSKSKDWNKYMTQGATTIHTINPNILIIISGLNFDNDLRCQRQNPLPLNNLHN 260
           RNELRG +S    W+++M  GA T+H  NP +L+I+SGL FD DL      P+ L     
Sbjct: 206 RNELRGNRSTPISWSRHMQWGAATVHKANPKVLVILSGLQFDTDLSFLPVLPVTL-PFKE 265

Query: 261 KLVFEVHLYSFSGESQSKFINNPLNKICSKIINGFVERAEFVMEGAE--AVPLFVSEFGF 320
           K+V+E H YSF    ++   N+    +C      F     FV   A   A PLF+SEFG 
Sbjct: 266 KIVYEGHWYSFGVPWRTGLPND----VCKNETGRFKSNVGFVTSSANATAAPLFMSEFGI 325

Query: 321 DQRGVNEADDRFLSCFRAHLAKKDLDWALWAWQGSYYYRQGKIEP----EEVFGVLNYNW 380
           DQR VN+ D+R+L+C  A+LA++DLDWALW   GSYYYR  K +P    EE +G  N++W
Sbjct: 326 DQRYVNDNDNRYLNCILAYLAEEDLDWALWTMGGSYYYRSDK-QPVKDFEETYGFFNHDW 385

Query: 381 TDVRNPHFSQMFQLLQTMLQDPNSNSSNTY-VMYHPQSGQCVQVKDMMDKQIYLNNCSNA 440
           + +RNP F    + +Q  +QDP       Y ++YHP SG CV+    +   ++L +C + 
Sbjct: 386 SRIRNPDFISRLKEIQQPIQDPYLAPGPYYQIIYHPASGLCVE--SGIGNTVHLGSCQSV 445

Query: 441 -SHWSHEGD-GTPIMLEATNFCLKANGNGLPPSLSSDCFG-EQSVWTAISDSKLHLAT-- 500
            S W+++     PI L  ++ C+   GNGLP  ++ +C     ++W+ +S ++L L T  
Sbjct: 446 RSRWNYDASVKGPIGLMGSSSCISTQGNGLPAIMTENCSAPNNTLWSTVSSAQLQLGTRV 505

Query: 501 LTKQG-NNGLCLEKESSNSTRIVMGSCVCVGNDSNCLQ--DTQAQWFELVVTN 536
           L K G    +CL  + S S  I    C+C+  DS+C    + + QWF+++ TN
Sbjct: 506 LGKDGKEKWMCL--DGSKSPLISTNECICI-TDSHCYPKLNPEKQWFKVITTN 547

[Close]

BLAST of Bhi12G000657 vs. ExPASy Swiss-Prot
Match: P19487 (Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=engXCA PE=1 SV=2)

HSP 1 Score: 78.2 bits (191), Expect = 3.2e-13
Identity = 95/385 (24.68%), Postives = 152/385 (39.48%), Query Frame = 0


Query: 12  LAFISFFSLSYSFPLSTNGRWIVDSAGRRMKLMCVN-WPSHTQSMLIEGLDRRPLKDLAD 71
           LA  +  + SYS     N R IVD +G+ ++L  VN +   T + ++ GL  R  KD+  
Sbjct: 16  LALAAGPAFSYSI---NNSRQIVDDSGKVVQLKGVNVFGFETGNHVMHGLWARNWKDMIV 75

Query: 72  EVVRLRFNCVRLTYATHMFTRYANRTVQENFDLLDLKASKMGLALHNPFILNMTVFNAYE 131
           ++  L FN VRL +         + T+  + D              N  +  +T     +
Sbjct: 76  QMQGLGFNAVRLPFCP---ATLRSDTMPASIDY-----------SRNADLQGLTSLQILD 135

Query: 132 AAVDILGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEGLRLVARRFYNKS 191
             +      G+ V+ D+H      C  + +    +    +   +WL  LR VA R+ N  
Sbjct: 136 KVIAEFNARGMYVLLDHHTPD---CAGISE---LWYTGSYTEAQWLADLRFVANRYKNVP 195

Query: 192 NVVAMSLRNELR-----GTKSKSKDWNKYMTQGATTIHTINPNILIIISGLNFDNDLRCQ 251
            V+ + L+NE       GT + + DWNK   +G+  +  + P  LI + G+  DN + C 
Sbjct: 196 YVLGLDLKNEPHGAATWGTGNAATDWNKAAERGSAAVLAVAPKWLIAVEGIT-DNPV-CS 255

Query: 252 RQ---------NPL---PLNNLHNKLVFEVHLYSFSGESQSKF--INNPLNKICSKIING 311
                       PL   PLN   N+L+   H+Y      QS F   N P N      +  
Sbjct: 256 TNGGIFWGGNLQPLACTPLNIPANRLLLAPHVYGPDVFVQSYFNDSNFPNN------MPA 315

Query: 312 FVERAEFVMEGAEAVPLFVSEFGFDQRGVNEADDRFLSCFRAHLAKKDLDWAL-WAWQGS 371
             ER      G  A  L + EFG      +  D  +      +L  K ++    W+W  +
Sbjct: 316 IWERHFGQFAGTHA--LLLGEFGGKYGEGDARDKTWQDALVKYLRSKGINQGFYWSWNPN 358

Query: 372 YYYRQGKIEPEEVFGVLNYNWTDVR 376
                      +  G+L  +WT VR
Sbjct: 376 ---------SGDTGGILRDDWTSVR 358

[Close]

BLAST of Bhi12G000657 vs. NCBI nr
Match: XP_038875504.1 (glycosyl hydrolase 5 family protein-like [Benincasa hispida])

HSP 1 Score: 1121.7 bits (2900), Expect = 0.0e+00
Identity = 537/537 (100.00%), Postives = 537/537 (100.00%), Query Frame = 0


Query: 1   MGNTTQINFVFLAFISFFSLSYSFPLSTNGRWIVDSAGRRMKLMCVNWPSHTQSMLIEGL 60
           MGNTTQINFVFLAFISFFSLSYSFPLSTNGRWIVDSAGRRMKLMCVNWPSHTQSMLIEGL
Sbjct: 1   MGNTTQINFVFLAFISFFSLSYSFPLSTNGRWIVDSAGRRMKLMCVNWPSHTQSMLIEGL 60

Query: 61  DRRPLKDLADEVVRLRFNCVRLTYATHMFTRYANRTVQENFDLLDLKASKMGLALHNPFI 120
           DRRPLKDLADEVVRLRFNCVRLTYATHMFTRYANRTVQENFDLLDLKASKMGLALHNPFI
Sbjct: 61  DRRPLKDLADEVVRLRFNCVRLTYATHMFTRYANRTVQENFDLLDLKASKMGLALHNPFI 120

Query: 121 LNMTVFNAYEAAVDILGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEGLR 180
           LNMTVFNAYEAAVDILGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEGLR
Sbjct: 121 LNMTVFNAYEAAVDILGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEGLR 180

Query: 181 LVARRFYNKSNVVAMSLRNELRGTKSKSKDWNKYMTQGATTIHTINPNILIIISGLNFDN 240
           LVARRFYNKSNVVAMSLRNELRGTKSKSKDWNKYMTQGATTIHTINPNILIIISGLNFDN
Sbjct: 181 LVARRFYNKSNVVAMSLRNELRGTKSKSKDWNKYMTQGATTIHTINPNILIIISGLNFDN 240

Query: 241 DLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFINNPLNKICSKIINGFVERAEFVM 300
           DLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFINNPLNKICSKIINGFVERAEFVM
Sbjct: 241 DLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFINNPLNKICSKIINGFVERAEFVM 300

Query: 301 EGAEAVPLFVSEFGFDQRGVNEADDRFLSCFRAHLAKKDLDWALWAWQGSYYYRQGKIEP 360
           EGAEAVPLFVSEFGFDQRGVNEADDRFLSCFRAHLAKKDLDWALWAWQGSYYYRQGKIEP
Sbjct: 301 EGAEAVPLFVSEFGFDQRGVNEADDRFLSCFRAHLAKKDLDWALWAWQGSYYYRQGKIEP 360

Query: 361 EEVFGVLNYNWTDVRNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVQVKDMMDK 420
           EEVFGVLNYNWTDVRNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVQVKDMMDK
Sbjct: 361 EEVFGVLNYNWTDVRNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVQVKDMMDK 420

Query: 421 QIYLNNCSNASHWSHEGDGTPIMLEATNFCLKANGNGLPPSLSSDCFGEQSVWTAISDSK 480
           QIYLNNCSNASHWSHEGDGTPIMLEATNFCLKANGNGLPPSLSSDCFGEQSVWTAISDSK
Sbjct: 421 QIYLNNCSNASHWSHEGDGTPIMLEATNFCLKANGNGLPPSLSSDCFGEQSVWTAISDSK 480

Query: 481 LHLATLTKQGNNGLCLEKESSNSTRIVMGSCVCVGNDSNCLQDTQAQWFELVVTNTW 538
           LHLATLTKQGNNGLCLEKESSNSTRIVMGSCVCVGNDSNCLQDTQAQWFELVVTNTW
Sbjct: 481 LHLATLTKQGNNGLCLEKESSNSTRIVMGSCVCVGNDSNCLQDTQAQWFELVVTNTW 537

[Close]

BLAST of Bhi12G000657 vs. NCBI nr
Match: KAA0055112.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1004.2 bits (2595), Expect = 4.2e-289
Identity = 478/538 (88.85%), Postives = 510/538 (94.80%), Query Frame = 0


Query: 1   MGNTTQINFVFLAFISFF-SLSYSFPLSTNGRWIVDSA-GRRMKLMCVNWPSHTQSMLIE 60
           MG TTQ +FV LAFISFF SLSYS PLSTNGRWIVDSA GRR+KL+CVNWPSHTQSMLIE
Sbjct: 1   MGITTQFSFVVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRPLKDLADEVVRLRFNCVRLTYATHMFTRYANRTVQENFDLLDLKASKMGLALHNP 120
           GLDRRPLKDLA+EV+RLRFNCVRLTYATHMFTRYANRTV+ENFDLLDL+ASK+GLALHNP
Sbjct: 61  GLDRRPLKDLANEVMRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKVGLALHNP 120

Query: 121 FILNMTVFNAYEAAVDILGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEG 180
           F+LNMT+F AYEA VD+LGTSGLMVIADNHISQP+WCCSL+DGNGFFGDRYFD EEWLEG
Sbjct: 121 FVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDSEEWLEG 180

Query: 181 LRLVARRFYNKSNVVAMSLRNELRGTKSKSKDWNKYMTQGATTIHTINPNILIIISGLNF 240
           LRLVARRFYNKS VVAMSLRNELRG  SKSKDWNKY+TQGATTIH INPNIL+IISGLNF
Sbjct: 181 LRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKYVTQGATTIHNINPNILVIISGLNF 240

Query: 241 DNDLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFINNPLNKICSKIINGFVERAEF 300
           DNDLRCQRQ PL LNNLHNKLVFEVHLYSFSGESQSKFI+NPLNKICSKIINGFV+RAEF
Sbjct: 241 DNDLRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVQRAEF 300

Query: 301 VMEGAEAVPLFVSEFGFDQRGVNEADDRFLSCFRAHLAKKDLDWALWAWQGSYYYRQGKI 360
           VMEGAEAVPLFVSEFG DQ GVNEADDRFLSCF AHL +KDLDWALW WQGSYYYRQGK+
Sbjct: 301 VMEGAEAVPLFVSEFGLDQTGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV 360

Query: 361 EPEEVFGVLNYNWTDVRNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVQVKDMM 420
           E EEVFGVLNYNW+DVRNP FSQMFQLLQTMLQDPNSNSSNTY+MYHPQSGQCVQV DM 
Sbjct: 361 ELEEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSSNTYLMYHPQSGQCVQVHDMK 420

Query: 421 DKQIYLNNCSNASHWSHEGDGTPIMLEATNFCLKANGNGLPPSLSSDCFGEQSVWTAISD 480
            K+I+L+NCSNASHWS+EGDGTPIML +TNFCLKANGNGLPPSLS DCFGEQS+WTAISD
Sbjct: 421 KKEIFLDNCSNASHWSYEGDGTPIMLASTNFCLKANGNGLPPSLSRDCFGEQSIWTAISD 480

Query: 481 SKLHLATLTKQGNNGLCLEKESSNSTRIVMGSCVCVGNDSNCLQDTQAQWFELVVTNT 537
           SKLHLATLTKQGNNG+CLEKESSNS+RI+M SCVCVG+DSNCLQDTQAQWF+LVVTNT
Sbjct: 481 SKLHLATLTKQGNNGMCLEKESSNSSRILMRSCVCVGSDSNCLQDTQAQWFQLVVTNT 538

[Close]

BLAST of Bhi12G000657 vs. NCBI nr
Match: XP_008467306.1 (PREDICTED: major extracellular endoglucanase-like [Cucumis melo])

HSP 1 Score: 1001.9 bits (2589), Expect = 2.1e-288
Identity = 477/538 (88.66%), Postives = 508/538 (94.42%), Query Frame = 0


Query: 1   MGNTTQINFVFLAFISFF-SLSYSFPLSTNGRWIVDSA-GRRMKLMCVNWPSHTQSMLIE 60
           MG TTQ +FV LAFI FF SLSYS PLSTNGRWIVDSA G R+KL+CVNWPSHTQSMLIE
Sbjct: 1   MGITTQFSFVVLAFICFFSSLSYSLPLSTNGRWIVDSATGHRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRPLKDLADEVVRLRFNCVRLTYATHMFTRYANRTVQENFDLLDLKASKMGLALHNP 120
           GLDRRPLKDLA+EV+RL+FNCVRLTYATHMFTRYANRTV+ENFDLLDL+ASK+GLALHNP
Sbjct: 61  GLDRRPLKDLANEVMRLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKVGLALHNP 120

Query: 121 FILNMTVFNAYEAAVDILGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEG 180
           F+LNMT+F AYEA VD+LGTSGLMVIADNHISQP+WCCSL+DGNGFFGDRYFD EEWLEG
Sbjct: 121 FVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDSEEWLEG 180

Query: 181 LRLVARRFYNKSNVVAMSLRNELRGTKSKSKDWNKYMTQGATTIHTINPNILIIISGLNF 240
           LRLVARRFYNKS VVAMSLRNELRG  SKSKDWNKY+TQGATTIH INPNIL+IISGLNF
Sbjct: 181 LRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKYVTQGATTIHNINPNILVIISGLNF 240

Query: 241 DNDLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFINNPLNKICSKIINGFVERAEF 300
           DNDLRCQRQ PL LNNLHNKLVFEVHLYSFSGESQSKFI+NPLNKICSKIINGFV+RAEF
Sbjct: 241 DNDLRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVQRAEF 300

Query: 301 VMEGAEAVPLFVSEFGFDQRGVNEADDRFLSCFRAHLAKKDLDWALWAWQGSYYYRQGKI 360
           VMEGAEAVPLFVSEFG DQ GVNEADDRFLSCF AHL +KDLDWALW WQGSYYYRQGK+
Sbjct: 301 VMEGAEAVPLFVSEFGLDQTGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV 360

Query: 361 EPEEVFGVLNYNWTDVRNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVQVKDMM 420
           E EEVFGVLNYNW+DVRNP FSQMFQLLQTMLQDPNSNSSNTY+MYHPQSGQCVQV DM 
Sbjct: 361 ELEEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSSNTYLMYHPQSGQCVQVHDMK 420

Query: 421 DKQIYLNNCSNASHWSHEGDGTPIMLEATNFCLKANGNGLPPSLSSDCFGEQSVWTAISD 480
            K+I+LNNCSNASHWS+EGDGTPIML +TNFCLKANGNGLPPSLS DCFGEQSVWTAISD
Sbjct: 421 QKEIFLNNCSNASHWSYEGDGTPIMLASTNFCLKANGNGLPPSLSRDCFGEQSVWTAISD 480

Query: 481 SKLHLATLTKQGNNGLCLEKESSNSTRIVMGSCVCVGNDSNCLQDTQAQWFELVVTNT 537
           SKLHLATLTKQGNNG+CLEKESSNS+RI+M SCVCVG+DSNCLQDTQAQWF+LVVTNT
Sbjct: 481 SKLHLATLTKQGNNGMCLEKESSNSSRILMRSCVCVGSDSNCLQDTQAQWFQLVVTNT 538

[Close]

BLAST of Bhi12G000657 vs. NCBI nr
Match: XP_004143723.1 (glycosyl hydrolase 5 family protein [Cucumis sativus] >KAE8647936.1 hypothetical protein Csa_000506 [Cucumis sativus])

HSP 1 Score: 1001.9 bits (2589), Expect = 2.1e-288
Identity = 477/535 (89.16%), Postives = 506/535 (94.58%), Query Frame = 0


Query: 4   TTQINFVFLAFISFF-SLSYSFPLSTNGRWIVDSA-GRRMKLMCVNWPSHTQSMLIEGLD 63
           TTQ  FV LAFISFF SLSYS PLSTNGRWIVDSA GRR+KL+CVNWPSHTQSMLIEGLD
Sbjct: 4   TTQFGFVVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLD 63

Query: 64  RRPLKDLADEVVRLRFNCVRLTYATHMFTRYANRTVQENFDLLDLKASKMGLALHNPFIL 123
           RRPLKDLA+EVVRLRFNCVRLTYATHMFTRYANRTV+ENFDLLDL+A+K+GLA HNPF+L
Sbjct: 64  RRPLKDLANEVVRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRAAKVGLAFHNPFVL 123

Query: 124 NMTVFNAYEAAVDILGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEGLRL 183
           NMT+F AYEA VD+LGTSGLMVIADNHISQP+WCCSL+DGNGFFGDRYFD EEWLEGLRL
Sbjct: 124 NMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDTEEWLEGLRL 183

Query: 184 VARRFYNKSNVVAMSLRNELRGTKSKSKDWNKYMTQGATTIHTINPNILIIISGLNFDND 243
           VARRFYNKS VVAMSLRNELRG  SKSKDWNKY+TQGATTIH INP IL+IISGLNFDND
Sbjct: 184 VARRFYNKSAVVAMSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDND 243

Query: 244 LRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFINNPLNKICSKIINGFVERAEFVME 303
           LRCQRQ PL LNNLHNKLVFEVHLYSFSGESQSKFI+NPLNKICSK+INGFVERAEFVME
Sbjct: 244 LRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVME 303

Query: 304 GAEAVPLFVSEFGFDQRGVNEADDRFLSCFRAHLAKKDLDWALWAWQGSYYYRQGKIEPE 363
           GAEAVPLFVSEFG DQRGVNEADDRFLSCF AHL +KDLDWALW WQGSYYYRQGK+ PE
Sbjct: 304 GAEAVPLFVSEFGLDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGPE 363

Query: 364 EVFGVLNYNWTDVRNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVQVKDMMDKQ 423
           EVFGVLNYNW+DVRNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCV V+DM   Q
Sbjct: 364 EVFGVLNYNWSDVRNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVLVQDMKHMQ 423

Query: 424 IYLNNCSNASHWSHEGDGTPIMLEATNFCLKANGNGLPPSLSSDCFGEQSVWTAISDSKL 483
           IYLN+CSNASHWS+EGDGTPIML +TNFCLKA+G+GLPPSLS DCFGEQSVWTAISDSKL
Sbjct: 424 IYLNDCSNASHWSYEGDGTPIMLASTNFCLKASGDGLPPSLSRDCFGEQSVWTAISDSKL 483

Query: 484 HLATLTKQGNNGLCLEKESSNSTRIVMGSCVCVGNDSNCLQDTQAQWFELVVTNT 537
           HLATLTKQGNNG+CLEKESSNS+RI+M SCVCVGNDSNCLQDTQAQWF+LVVTNT
Sbjct: 484 HLATLTKQGNNGMCLEKESSNSSRILMRSCVCVGNDSNCLQDTQAQWFQLVVTNT 538

[Close]

BLAST of Bhi12G000657 vs. NCBI nr
Match: XP_022995241.1 (uncharacterized protein LOC111490847 [Cucurbita maxima])

HSP 1 Score: 929.9 bits (2402), Expect = 1.0e-266
Identity = 444/538 (82.53%), Postives = 487/538 (90.52%), Query Frame = 0


Query: 1   MGNTTQINFVFLAFISFF-SLSYSFPLSTNGRWIVDSA-GRRMKLMCVNWPSHTQSMLIE 60
           MG+ TQ  F+ LAFIS F SLS S PLST GRWIVDSA GRR+KL+CVNWPSHTQSMLIE
Sbjct: 1   MGSPTQ-TFMLLAFISLFSSLSNSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRPLKDLADEVVRLRFNCVRLTYATHMFTRYANRTVQENFDLLDLKASKMGLALHNP 120
           GL  RPLKDLADE+V L+FNCVRLTYATHMFTRYANRTV+ENFDLLDL+ASK GLA HNP
Sbjct: 61  GLAHRPLKDLADELVNLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKAGLASHNP 120

Query: 121 FILNMTVFNAYEAAVDILGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEG 180
           FILNMT+F+AYEA VD+LGTSGLMVIADNHISQP+WCCSL+DGNGFFGDRYFDP+EWLEG
Sbjct: 121 FILNMTIFDAYEAVVDVLGTSGLMVIADNHISQPRWCCSLNDGNGFFGDRYFDPQEWLEG 180

Query: 181 LRLVARRFYNKSNVVAMSLRNELRGTKSKSKDWNKYMTQGATTIHTINPNILIIISGLNF 240
           LRLVARRF NK NVVAMSLRNELRG KS SKDWNKYMTQGATTIH INPN+L+I+SGLNF
Sbjct: 181 LRLVARRFTNKLNVVAMSLRNELRGAKSSSKDWNKYMTQGATTIHDINPNLLVIVSGLNF 240

Query: 241 DNDLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFINNPLNKICSKIINGFVERAEF 300
           DNDLRCQR NPLPLNNLHNKLVFEVHLYSFSG +++KFI NPLNKICS IINGFVERAEF
Sbjct: 241 DNDLRCQRHNPLPLNNLHNKLVFEVHLYSFSGATKTKFITNPLNKICSAIINGFVERAEF 300

Query: 301 VMEGAEAVPLFVSEFGFDQRGVNEADDRFLSCFRAHLAKKDLDWALWAWQGSYYYRQGKI 360
           VM+GAEAVPLFVSEFGFDQRG N ADDRF SCF AHLA+ DLDWALWAWQGSYYYRQG+ 
Sbjct: 301 VMQGAEAVPLFVSEFGFDQRGTNVADDRFSSCFVAHLARTDLDWALWAWQGSYYYRQGQA 360

Query: 361 EPEEVFGVLNYNWTDVRNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVQVKDMM 420
           + +EVFGVLNYNW+DVRNPHFS+ FQLLQTML+DPNSN+ N+YVMYHPQSGQCV+VKDMM
Sbjct: 361 QSDEVFGVLNYNWSDVRNPHFSKTFQLLQTMLRDPNSNAPNSYVMYHPQSGQCVRVKDMM 420

Query: 421 DKQIYLNNCSNASHWSHEGDGTPIMLEATNFCLKANGNGLPPSLSSDCFGEQSVWTAISD 480
            K IYLN+CSNASHWSH GDGTPI LEAT+ CLKA+ +GL P LS DC G++S WT IS+
Sbjct: 421 SKDIYLNDCSNASHWSHRGDGTPIELEATSLCLKADRDGLRPLLSRDCSGDESAWTTISN 480

Query: 481 SKLHLATLTKQGNNGLCLEKESSNSTRIVMGSCVCVGNDSNCLQDTQAQWFELVVTNT 537
           SKLHLAT T+QG NGLCLEKESSNSTRIVMG C+CVG+DSNCL DT++QWFELV TNT
Sbjct: 481 SKLHLATFTRQG-NGLCLEKESSNSTRIVMGRCLCVGDDSNCLDDTRSQWFELVGTNT 536

[Close]

BLAST of Bhi12G000657 vs. ExPASy TrEMBL
Match: A0A5A7UH07 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00380 PE=3 SV=1)

HSP 1 Score: 1004.2 bits (2595), Expect = 2.1e-289
Identity = 478/538 (88.85%), Postives = 510/538 (94.80%), Query Frame = 0


Query: 1   MGNTTQINFVFLAFISFF-SLSYSFPLSTNGRWIVDSA-GRRMKLMCVNWPSHTQSMLIE 60
           MG TTQ +FV LAFISFF SLSYS PLSTNGRWIVDSA GRR+KL+CVNWPSHTQSMLIE
Sbjct: 1   MGITTQFSFVVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRPLKDLADEVVRLRFNCVRLTYATHMFTRYANRTVQENFDLLDLKASKMGLALHNP 120
           GLDRRPLKDLA+EV+RLRFNCVRLTYATHMFTRYANRTV+ENFDLLDL+ASK+GLALHNP
Sbjct: 61  GLDRRPLKDLANEVMRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKVGLALHNP 120

Query: 121 FILNMTVFNAYEAAVDILGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEG 180
           F+LNMT+F AYEA VD+LGTSGLMVIADNHISQP+WCCSL+DGNGFFGDRYFD EEWLEG
Sbjct: 121 FVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDSEEWLEG 180

Query: 181 LRLVARRFYNKSNVVAMSLRNELRGTKSKSKDWNKYMTQGATTIHTINPNILIIISGLNF 240
           LRLVARRFYNKS VVAMSLRNELRG  SKSKDWNKY+TQGATTIH INPNIL+IISGLNF
Sbjct: 181 LRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKYVTQGATTIHNINPNILVIISGLNF 240

Query: 241 DNDLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFINNPLNKICSKIINGFVERAEF 300
           DNDLRCQRQ PL LNNLHNKLVFEVHLYSFSGESQSKFI+NPLNKICSKIINGFV+RAEF
Sbjct: 241 DNDLRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVQRAEF 300

Query: 301 VMEGAEAVPLFVSEFGFDQRGVNEADDRFLSCFRAHLAKKDLDWALWAWQGSYYYRQGKI 360
           VMEGAEAVPLFVSEFG DQ GVNEADDRFLSCF AHL +KDLDWALW WQGSYYYRQGK+
Sbjct: 301 VMEGAEAVPLFVSEFGLDQTGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV 360

Query: 361 EPEEVFGVLNYNWTDVRNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVQVKDMM 420
           E EEVFGVLNYNW+DVRNP FSQMFQLLQTMLQDPNSNSSNTY+MYHPQSGQCVQV DM 
Sbjct: 361 ELEEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSSNTYLMYHPQSGQCVQVHDMK 420

Query: 421 DKQIYLNNCSNASHWSHEGDGTPIMLEATNFCLKANGNGLPPSLSSDCFGEQSVWTAISD 480
            K+I+L+NCSNASHWS+EGDGTPIML +TNFCLKANGNGLPPSLS DCFGEQS+WTAISD
Sbjct: 421 KKEIFLDNCSNASHWSYEGDGTPIMLASTNFCLKANGNGLPPSLSRDCFGEQSIWTAISD 480

Query: 481 SKLHLATLTKQGNNGLCLEKESSNSTRIVMGSCVCVGNDSNCLQDTQAQWFELVVTNT 537
           SKLHLATLTKQGNNG+CLEKESSNS+RI+M SCVCVG+DSNCLQDTQAQWF+LVVTNT
Sbjct: 481 SKLHLATLTKQGNNGMCLEKESSNSSRILMRSCVCVGSDSNCLQDTQAQWFQLVVTNT 538

[Close]

BLAST of Bhi12G000657 vs. ExPASy TrEMBL
Match: A0A1S3CTF8 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103504686 PE=3 SV=1)

HSP 1 Score: 1001.9 bits (2589), Expect = 1.0e-288
Identity = 477/538 (88.66%), Postives = 508/538 (94.42%), Query Frame = 0


Query: 1   MGNTTQINFVFLAFISFF-SLSYSFPLSTNGRWIVDSA-GRRMKLMCVNWPSHTQSMLIE 60
           MG TTQ +FV LAFI FF SLSYS PLSTNGRWIVDSA G R+KL+CVNWPSHTQSMLIE
Sbjct: 1   MGITTQFSFVVLAFICFFSSLSYSLPLSTNGRWIVDSATGHRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRPLKDLADEVVRLRFNCVRLTYATHMFTRYANRTVQENFDLLDLKASKMGLALHNP 120
           GLDRRPLKDLA+EV+RL+FNCVRLTYATHMFTRYANRTV+ENFDLLDL+ASK+GLALHNP
Sbjct: 61  GLDRRPLKDLANEVMRLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKVGLALHNP 120

Query: 121 FILNMTVFNAYEAAVDILGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEG 180
           F+LNMT+F AYEA VD+LGTSGLMVIADNHISQP+WCCSL+DGNGFFGDRYFD EEWLEG
Sbjct: 121 FVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDSEEWLEG 180

Query: 181 LRLVARRFYNKSNVVAMSLRNELRGTKSKSKDWNKYMTQGATTIHTINPNILIIISGLNF 240
           LRLVARRFYNKS VVAMSLRNELRG  SKSKDWNKY+TQGATTIH INPNIL+IISGLNF
Sbjct: 181 LRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKYVTQGATTIHNINPNILVIISGLNF 240

Query: 241 DNDLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFINNPLNKICSKIINGFVERAEF 300
           DNDLRCQRQ PL LNNLHNKLVFEVHLYSFSGESQSKFI+NPLNKICSKIINGFV+RAEF
Sbjct: 241 DNDLRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVQRAEF 300

Query: 301 VMEGAEAVPLFVSEFGFDQRGVNEADDRFLSCFRAHLAKKDLDWALWAWQGSYYYRQGKI 360
           VMEGAEAVPLFVSEFG DQ GVNEADDRFLSCF AHL +KDLDWALW WQGSYYYRQGK+
Sbjct: 301 VMEGAEAVPLFVSEFGLDQTGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV 360

Query: 361 EPEEVFGVLNYNWTDVRNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVQVKDMM 420
           E EEVFGVLNYNW+DVRNP FSQMFQLLQTMLQDPNSNSSNTY+MYHPQSGQCVQV DM 
Sbjct: 361 ELEEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSSNTYLMYHPQSGQCVQVHDMK 420

Query: 421 DKQIYLNNCSNASHWSHEGDGTPIMLEATNFCLKANGNGLPPSLSSDCFGEQSVWTAISD 480
            K+I+LNNCSNASHWS+EGDGTPIML +TNFCLKANGNGLPPSLS DCFGEQSVWTAISD
Sbjct: 421 QKEIFLNNCSNASHWSYEGDGTPIMLASTNFCLKANGNGLPPSLSRDCFGEQSVWTAISD 480

Query: 481 SKLHLATLTKQGNNGLCLEKESSNSTRIVMGSCVCVGNDSNCLQDTQAQWFELVVTNT 537
           SKLHLATLTKQGNNG+CLEKESSNS+RI+M SCVCVG+DSNCLQDTQAQWF+LVVTNT
Sbjct: 481 SKLHLATLTKQGNNGMCLEKESSNSSRILMRSCVCVGSDSNCLQDTQAQWFQLVVTNT 538

[Close]

BLAST of Bhi12G000657 vs. ExPASy TrEMBL
Match: A0A6J1K1F7 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111490847 PE=3 SV=1)

HSP 1 Score: 929.9 bits (2402), Expect = 4.9e-267
Identity = 444/538 (82.53%), Postives = 487/538 (90.52%), Query Frame = 0


Query: 1   MGNTTQINFVFLAFISFF-SLSYSFPLSTNGRWIVDSA-GRRMKLMCVNWPSHTQSMLIE 60
           MG+ TQ  F+ LAFIS F SLS S PLST GRWIVDSA GRR+KL+CVNWPSHTQSMLIE
Sbjct: 1   MGSPTQ-TFMLLAFISLFSSLSNSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRPLKDLADEVVRLRFNCVRLTYATHMFTRYANRTVQENFDLLDLKASKMGLALHNP 120
           GL  RPLKDLADE+V L+FNCVRLTYATHMFTRYANRTV+ENFDLLDL+ASK GLA HNP
Sbjct: 61  GLAHRPLKDLADELVNLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKAGLASHNP 120

Query: 121 FILNMTVFNAYEAAVDILGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEG 180
           FILNMT+F+AYEA VD+LGTSGLMVIADNHISQP+WCCSL+DGNGFFGDRYFDP+EWLEG
Sbjct: 121 FILNMTIFDAYEAVVDVLGTSGLMVIADNHISQPRWCCSLNDGNGFFGDRYFDPQEWLEG 180

Query: 181 LRLVARRFYNKSNVVAMSLRNELRGTKSKSKDWNKYMTQGATTIHTINPNILIIISGLNF 240
           LRLVARRF NK NVVAMSLRNELRG KS SKDWNKYMTQGATTIH INPN+L+I+SGLNF
Sbjct: 181 LRLVARRFTNKLNVVAMSLRNELRGAKSSSKDWNKYMTQGATTIHDINPNLLVIVSGLNF 240

Query: 241 DNDLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFINNPLNKICSKIINGFVERAEF 300
           DNDLRCQR NPLPLNNLHNKLVFEVHLYSFSG +++KFI NPLNKICS IINGFVERAEF
Sbjct: 241 DNDLRCQRHNPLPLNNLHNKLVFEVHLYSFSGATKTKFITNPLNKICSAIINGFVERAEF 300

Query: 301 VMEGAEAVPLFVSEFGFDQRGVNEADDRFLSCFRAHLAKKDLDWALWAWQGSYYYRQGKI 360
           VM+GAEAVPLFVSEFGFDQRG N ADDRF SCF AHLA+ DLDWALWAWQGSYYYRQG+ 
Sbjct: 301 VMQGAEAVPLFVSEFGFDQRGTNVADDRFSSCFVAHLARTDLDWALWAWQGSYYYRQGQA 360

Query: 361 EPEEVFGVLNYNWTDVRNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVQVKDMM 420
           + +EVFGVLNYNW+DVRNPHFS+ FQLLQTML+DPNSN+ N+YVMYHPQSGQCV+VKDMM
Sbjct: 361 QSDEVFGVLNYNWSDVRNPHFSKTFQLLQTMLRDPNSNAPNSYVMYHPQSGQCVRVKDMM 420

Query: 421 DKQIYLNNCSNASHWSHEGDGTPIMLEATNFCLKANGNGLPPSLSSDCFGEQSVWTAISD 480
            K IYLN+CSNASHWSH GDGTPI LEAT+ CLKA+ +GL P LS DC G++S WT IS+
Sbjct: 421 SKDIYLNDCSNASHWSHRGDGTPIELEATSLCLKADRDGLRPLLSRDCSGDESAWTTISN 480

Query: 481 SKLHLATLTKQGNNGLCLEKESSNSTRIVMGSCVCVGNDSNCLQDTQAQWFELVVTNT 537
           SKLHLAT T+QG NGLCLEKESSNSTRIVMG C+CVG+DSNCL DT++QWFELV TNT
Sbjct: 481 SKLHLATFTRQG-NGLCLEKESSNSTRIVMGRCLCVGDDSNCLDDTRSQWFELVGTNT 536

[Close]

BLAST of Bhi12G000657 vs. ExPASy TrEMBL
Match: A0A6J1H201 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459707 PE=3 SV=1)

HSP 1 Score: 923.7 bits (2386), Expect = 3.5e-265
Identity = 440/538 (81.78%), Postives = 484/538 (89.96%), Query Frame = 0


Query: 1   MGNTTQINFVFLAFISFF-SLSYSFPLSTNGRWIVDS-AGRRMKLMCVNWPSHTQSMLIE 60
           MG+ +Q   + LAFIS F SLS S PLST GRWIVDS  GRR+KL+CVNWPSHTQSMLIE
Sbjct: 1   MGSPSQ-TLMLLAFISLFSSLSNSLPLSTRGRWIVDSTTGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRPLKDLADEVVRLRFNCVRLTYATHMFTRYANRTVQENFDLLDLKASKMGLALHNP 120
           GL  RPLKDLADE+V L+FNCVRLTYATHMFTRYANRTV+ENFDLLDL+ASK+GLA HNP
Sbjct: 61  GLANRPLKDLADELVSLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKVGLASHNP 120

Query: 121 FILNMTVFNAYEAAVDILGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEG 180
           F+LNMT+F AYE  VD+LGTSGLMVIADNHISQP+WCCSL+DGNGFFGDRYFDP+EWLEG
Sbjct: 121 FVLNMTIFEAYETVVDVLGTSGLMVIADNHISQPRWCCSLNDGNGFFGDRYFDPQEWLEG 180

Query: 181 LRLVARRFYNKSNVVAMSLRNELRGTKSKSKDWNKYMTQGATTIHTINPNILIIISGLNF 240
           LRLVARRF NKSNVVAMSLRNELRG KS SKDWNKYMTQGATTIH INPN+L+I+SGLNF
Sbjct: 181 LRLVARRFTNKSNVVAMSLRNELRGAKSSSKDWNKYMTQGATTIHDINPNLLVIVSGLNF 240

Query: 241 DNDLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFINNPLNKICSKIINGFVERAEF 300
           DNDLRCQR NPLPLNNLHNKLVFEVHLYSFSG +++KFI NPLNKICS IINGFVERAEF
Sbjct: 241 DNDLRCQRHNPLPLNNLHNKLVFEVHLYSFSGATKTKFITNPLNKICSTIINGFVERAEF 300

Query: 301 VMEGAEAVPLFVSEFGFDQRGVNEADDRFLSCFRAHLAKKDLDWALWAWQGSYYYRQGKI 360
           VM+GAEAVPLFVSEFGFDQRG N ADDRFLSCF AHLAK DLDWALWAWQGSYYYRQG+ 
Sbjct: 301 VMQGAEAVPLFVSEFGFDQRGTNVADDRFLSCFVAHLAKTDLDWALWAWQGSYYYRQGQA 360

Query: 361 EPEEVFGVLNYNWTDVRNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVQVKDMM 420
           + +EVFG+LNYNW+ VRNP FS+ FQLLQTML+DPNSN+ N+YVMYHPQSGQCV+VKDMM
Sbjct: 361 QFDEVFGILNYNWSGVRNPRFSKTFQLLQTMLRDPNSNAPNSYVMYHPQSGQCVRVKDMM 420

Query: 421 DKQIYLNNCSNASHWSHEGDGTPIMLEATNFCLKANGNGLPPSLSSDCFGEQSVWTAISD 480
            K+IYLN+CSNASHWSH GDGTPI LEAT  CLKA+G+GL P LS DC   +S WT IS+
Sbjct: 421 SKEIYLNDCSNASHWSHRGDGTPIELEATGLCLKADGDGLRPLLSRDCLSNESSWTTISN 480

Query: 481 SKLHLATLTKQGNNGLCLEKESSNSTRIVMGSCVCVGNDSNCLQDTQAQWFELVVTNT 537
           SKLHLATLT+ G NGLCLEK+SSNSTRIVMG CVCVG+DSNCL DT++QWFELVVTNT
Sbjct: 481 SKLHLATLTRHG-NGLCLEKDSSNSTRIVMGRCVCVGDDSNCLDDTRSQWFELVVTNT 536

[Close]

BLAST of Bhi12G000657 vs. ExPASy TrEMBL
Match: A0A6J1DXU9 (Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111025142 PE=3 SV=1)

HSP 1 Score: 873.6 bits (2256), Expect = 4.2e-250
Identity = 422/541 (78.00%), Postives = 470/541 (86.88%), Query Frame = 0


Query: 1   MGNTTQINFVFLAFISFF-SLSYSFPLSTNGRWIVDSA-GRRMKLMCVNWPSHTQSMLIE 60
           MG+T +  FVFLA IS   SLSYS PLST GRWIVDSA GRR+KL+CVNWPSHTQSMLIE
Sbjct: 1   MGSTKE-TFVFLALISLLSSLSYSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRPLKDLADEVVRLRFNCVRLTYATHMFTRYANRTVQENFDLLDLKASKMGLALHNP 120
           GLDRRPLKDLADE VRLRFNCVRLTYATHMFTRYANRT++ENFDLLDLKASK GLALHNP
Sbjct: 61  GLDRRPLKDLADEAVRLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKASKAGLALHNP 120

Query: 121 FILNMTVFNAYEAAVDILGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEG 180
           F+LNMT+F AYEAAVD+LG SGLMVIADNHISQP+WCCSLDDGNGFFGDRYFDPEEWLEG
Sbjct: 121 FVLNMTIFEAYEAAVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPEEWLEG 180

Query: 181 LRLVARRFYNKSNVVAMSLRNELRGTKSKSKDWNKYMTQGATTIHTINPNILIIISGLNF 240
           LRLVAR F+NKS+VV MSLRNELRG +S  KDWNK++T+GA  IHTINPN L+IISGLN+
Sbjct: 181 LRLVARHFHNKSSVVGMSLRNELRGARSNPKDWNKHITRGARAIHTINPNALVIISGLNY 240

Query: 241 DNDLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFINNPLNKICSKIINGFVERAEF 300
           DNDLRCQ Q PLPL NLHNKLV EVHLYSFSG+SQ+KF  NPLNKICSK++NGFVERA F
Sbjct: 241 DNDLRCQTQAPLPLGNLHNKLVLEVHLYSFSGDSQAKFTQNPLNKICSKVMNGFVERAGF 300

Query: 301 VMEGAEAVPLFVSEFGFDQR-GVNEADDRFLSCFRAHLAKKDLDWALWAWQGSYYYRQGK 360
           V+EG  AVPL VSEFG DQR   NEADDRF+SCF A+LA+ DLDWA+W WQGSYYYRQG+
Sbjct: 301 VVEGERAVPLLVSEFGMDQRESGNEADDRFMSCFGAYLARMDLDWAVWGWQGSYYYRQGQ 360

Query: 361 IEPEEVFGVLNYNWTDVRNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVQVKDM 420
             P EVFGVLNY+W+ VRNP FSQ FQLLQTMLQDPNS S N+Y+MYHPQSGQCVQ +  
Sbjct: 361 ARPREVFGVLNYDWSGVRNPRFSQRFQLLQTMLQDPNSYSPNSYLMYHPQSGQCVQAEG- 420

Query: 421 MDKQIYLNNCSNASHWSHEGDGTPIMLEATNFCLKANGNGLPPSLSSDCFGEQSVWTAIS 480
             K+IY+NNCS+AS WSHEGDGTPI L+ +  CL+A+G GL PSLS++C  + S W AIS
Sbjct: 421 NSKEIYMNNCSHASQWSHEGDGTPIRLKDSGLCLRASGYGLKPSLSTECLYKHSGWRAIS 480

Query: 481 DSKLHLATLTKQGN-NG-LCLEKESSNSTRIVMGSCVCVGNDSNCLQDTQAQWFELVVTN 537
           DSKLHLATL   GN NG LCLE+ESSNSTRIVMG C+CVG+DS+CL DTQAQWF+LVVTN
Sbjct: 481 DSKLHLATLAPNGNGNGNLCLERESSNSTRIVMGRCICVGDDSSCLHDTQAQWFQLVVTN 539

The following BLAST results are available for this feature:

BLAST of Bhi12G000657 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G13130.1	7.6e-119	40.86	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT3G26130.1	1.0e-115	42.13	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT3G26140.1	2.9e-110	39.92	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT5G17500.1	8.4e-102	37.88	Glycosyl hydrolase superfamily protein	[more]
AT5G16700.1	1.8e-83	34.64	Glycosyl hydrolase superfamily protein	[more]

BLAST of Bhi12G000657 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C0HLA0	1.3e-115	42.96	Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]
P19487	3.2e-13	24.68	Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (stra...	[more]

BLAST of Bhi12G000657 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038875504.1	0.0e+00	100.00	glycosyl hydrolase 5 family protein-like [Benincasa hispida]	[more]
KAA0055112.1	4.2e-289	88.85	major extracellular endoglucanase-like [Cucumis melo var. makuwa]	[more]
XP_008467306.1	2.1e-288	88.66	PREDICTED: major extracellular endoglucanase-like [Cucumis melo]	[more]
XP_004143723.1	2.1e-288	89.16	glycosyl hydrolase 5 family protein [Cucumis sativus] >KAE8647936.1 hypothetical...	[more]
XP_022995241.1	1.0e-266	82.53	uncharacterized protein LOC111490847 [Cucurbita maxima]	[more]

BLAST of Bhi12G000657 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UH07	2.1e-289	88.85	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A1S3CTF8	1.0e-288	88.66	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103504686 PE=3 SV...	[more]
A0A6J1K1F7	4.9e-267	82.53	Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111490847 PE=...	[more]
A0A6J1H201	3.5e-265	81.78	Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459707 P...	[more]
A0A6J1DXU9	4.2e-250	78.00	Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111025142 ...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001547	Glycoside hydrolase, family 5	PFAM	PF00150	Cellulase	coord: 64..348 e-value: 1.0E-25 score: 90.8
None	No IPR available	GENE3D	2.80.10.50		coord: 401..527 e-value: 5.6E-5 score: 25.1
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 24..392 e-value: 1.1E-68 score: 234.1
None	No IPR available	PANTHER	PTHR31263	CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)	coord: 9..533
IPR000772	Ricin B, lectin domain	PROSITE	PS50231	RICIN_B_LECTIN	coord: 398..531 score: 9.2445
IPR035992	Ricin B-like lectins	SUPERFAMILY	50370	Ricin B-like lectins	coord: 396..517
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 25..386

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi12M000657	Bhi12M000657	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071704	organic substance metabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0016985	mannan endo-1,4-beta-mannosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds