Bhi12G000559 (gene) Wax gourd (B227) v1

Overview
NameBhi12G000559
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionPectinesterase
Locationchr12: 19483370 .. 19488962 (+)
RNA-Seq ExpressionBhi12G000559
SyntenyBhi12G000559
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTAAACTCATTCACTCCCATTATTCCCTCCTCAACCCCATGCACACTCATCTCATTATTCTCCTTCAACAACAACAACAACCATGATCAACAACAACTACAACAAGCTCCACTCCTCCCTCTCCGCCACCTCTACTCCGGCATCCTCCTTCCACCTCTCCGGCAATGATCTCCCGCCCACACCTCCACCCTCCTCCTCCCACCGCCGTCGCTCTCGTATCCTTATCATCTCCTTCCTCTCCGTCCTCCTCATCGCTGCCTCCATCGTCTCTGCCGCCGTCCTAGTCCGGTCCCGCATCGCGTCTAACGAACCCCATTTCCCACACAACAAGCCCTCCCAAGCCATCTCCCGCGCGTGTAGCCACACTCTCTACCCATCTCTCTGCCTCTCATCTCTTCTCTCCATCCCCGACGCGCTCTCCGCCGACGTTCATGACCTTGTTCATATCTCTCTCAACTTAACTCTTCAACATCTCACTAAGGCTCTTTATTCCACTTCTCAAATCCCTATCTCACAAATCTCTAACGACCCTTTAGCTCATTCAGCTTATGAAGACTGTATGGAGCTTCTAAATGACGCCGTTGACGCTTTTTCAGTTTCTCTTTTTTCTACTGAAGCTTCCAATCACGACGTCATGACGTGGCTTAGTGCGGCATTGACTTACCATGACACGTGTACGGATGGATTTGCGGATGTGGCTGACTTGGCTGTTAAGAATCAGGTGGAGGCGAAGTTGAGTGATTTGTCGGAGATGATCAGTAATTCTTTGGCTATATTTTCTGGGTTTGGTGGTGGGGATTTGCCAGTGCAGAATAGGCGGCGTCGGTTGATGGAGAGTGGTACGCGGTCATGGGCGGCGGAGAATGGTGGTGATGGGGGTTTTCCTCAATGGCTGAGTGTGAAGGATCGGCGGCTTTTGGCTGCTCCTATATCGGCGATTCAGGCGGATATTGTGGTGGCTAAGGATGGTTCTGGGAAGTTCAAGACGGTGGCTGAGGCCATTGACGCGGCGCCGTCGTCGAGTGGCCGCCGGATCGTCATCTACATTAAGGCCGGAAAGTAAGAACAATACTTTTCTCTACTAATTCAAAATGAGAGTATATATATTTTCACTAATTAAATTAGATTATAATTATTTTAATATTTATAAACAAAAATTGTTTTTCTCAATACATCAATATTTATATAATTTACACAAATGTCCAATTATAAGTTGTCACATTATACTTTTTTTTTAAACCGATATTTCTACTTAAGGGAAAACTTAGTTACAAATTACACTCAATTAAAAATTATTAAGTGACAAATTTATTTTTTTAAATGATTAAAAAATTTAAAAATATATATTTCTTTTTCTCTTTGTGATAATCACTTTTTCCCTTTGTGATGGATGATGGGAATATATGAAAGAGATGATGAGTATTACTCTTTACAAACCTATTTTAGCATTTGATTAAAGCATTTTTTAAAAAATGTAATAGGTAGATTTCAAAATTTTAGTTTTAAGAAGATAGAAATCTAGGTGGACTAAGTTAGTTGGATTATTATAGAGTGGATCATGATGTTTTATTATTTTTCTTTGTTTTTTCCAAAACATGAATGATTGTTTATTTATTCCTTTTTTTCTTGGGAAAATGGAGAAAGAAAATGCTTGTGATTAGGCCATAATTAATCAACATTAGCTCTCATTAAATGGAAGGAAAGAAGAGGAAAAAAAAGTTTCTTTTAACAACCTTTTTCCTGATATGTGTTTAATTTATTGACTTCAGTGTCATAATCATGCAAAGCTGTTACTTACCAATTCCTAAAGTCCATAATTTTCGTGATAATAATTATACAATCAAATAAATATTACATGTCCCCACATTAATTTAAGTCCTTTTCAATTTAGTTGCTTTCACAATAATAACTCTTAACAACCAAATTAAACACAATAAAACTAACATGAATAAATTCAAATTAATTCGTATGTAGACCGATAACGAATATAAAAATAGTTTAGAAAGTGAGAGATCAAATCAATAAAATACAATTTAGTAGAAAAGGATTACTCATTCCAACTATCCATCACGAGAATCATAATATATTTAAAGACCGACATTTTTAGGGCAAATCATTTATTTATTTGGGTTACGATAATAATTGAAAATAATACCGTGGGACTAAGAAGGGATATTAATGTAATTTTGTAGGTACGAAGAGGAGAATTTGAAGGTGGGAAGGAAGAAAACAAACTTGATGTTTGTGGGAGATGGGAAGGGCATAACCGTCATTTCAGGTGGCAAAAGTGTTTTTGACAAAGTTACCACTTTTCGCACCGCCACCTTTGGTATATTCTCTCATTCTCTTTTCTCCTTTTTAATTTTATTCCACAACTTTAAAAAAAAAAAATTCTCTTGATATATTTAAATAAAAAAAAGTTAAAATACCAATTCGGTTAACGTCATTTAAAGTTTTGAGTTTTAATCCTTCAAAACATATTTTTTTATTGAAATTGATTAAATGATAATAATTATTGTGAATATATTTTTAAAAATTATAGTCAAAAGATTAATAATAAAAAGTTGGTTAATATTCCTACTTTCAAATGTCTGATTTCTCAAATTTAATTTTAATAAAATATAATAGGTTAATATATGACTAAATCTATAATGAAAATGTTAATAAATAATAATTTTTTTAAAACATCAATTATAAACTAGTAATAAGGACTAAATTTTAAATTTATTAAATATAATAAACTTAAAATTCAATATTTAAAGCAACAGTTACTAAAATCTTACAATAACTAAAATAGTATTTTTAATTTTCTTAAAAATTAAAGTACAAAATTTAGATTTTTTTTTAGAAAAAATTATGTGCAATAAAATATCAAGCCTTTTAATTCTGACACCGGATATCTAGGACTTAGAATTATGGTCGTCCGGTCTTGGAACTAAATGTGGCCATACGCTTTCATTCTTGTTTTCTTTTTCCACTTAAACATCATTTTTTTTTTTATTGAATTTTCAAAATTATCGCATATGATCTATTTTTAGTTTTTTAAAAAGTATACTATAATAATTCTTTTTTAAAAAAAATTGAATTTTCAGCTAAATTAAAAAAAAAAATCCATAAATTTTAAAACTATTTTATTTATATTAATGAAGATTGTTGAATGGATATTTGGTGAGAAGATTATAGGTGGTAGAATAGTAGCTTAAGTCGAAAATTTATTTTAGGTGGTAGAATAATAGCTAAGTTGAATTTAGGACCAAAGTAGCATTAAATATTTTTTGGAATAAATAATTATTACACATTTTAAGAAGAAAAATTTAGATGCATGTTTTTTATTGTAGAGAAAAATGGAGGCTATAAATTTTTGTTTAAAGAAAATTATATTTCAAGATAGTGACAAATTAAGCTACTTTTGTAGCATTTAATGGGCTACAAAAAATAAAAAATAATTGGTTTGATTTATCCAAATAATCATATGAGAAATGGTAGATTCCAGCTGTGTTAGGCAATTAGCCTTTTTTCTTCTTCTACGCATCATTAATTATTTTTGTAGGAAAAGCGCCCACATTCATTTTTATAATTATAAGCTATGTTATTTAATCGGTACAATTACTAGTAAAAGTCAAGCTTACAAATTGAATATTTGATGTAACATTTTCACATGCCATATTATAAATATATATCATAAAGATTTCCGTACTTGTGACAATAATATAAAAAGTTGATCATCTTTAGAAAAAAAAAATATAATAGATAGTAACAATTACGCAAGTTGTATCGATTGGTCATTACATGTAATAAATTTTCACATACCATATTATAAGAAGATTTAAAATCTTTTTGTTCTCTTTATAAGAAAGATGATGTCTCTATTACATAGTGTGTAATGTCTATAATAATTAGTTAGTAAAGTAGGCCCTTCTAATAAATTATTATAAGATTCAAAATATTTTACAAAGAAGATGAGAATTACAAAAAAGAAAAAAGAGAAAAAGAAAGGAATGGAATATCCCAATTATTCATGATGACTTTGAAAAGTCATACTAGTATTATTCTATGTTCTTGCATCATGCTTACTTCCACAACTTGGTAAGAATTTTTTTTTTTTTAAAAAAATAATAATATGAGAATCTAATGTTGTCCAAATAAAGAAGCCAAAAGCTTACTCCATGGAGATAAGGATATAAGTTAAGAAATTATTGTAATTCTATTTCAAAAGTGCTCCATCTTTCATCACATAATTTATGGTCCTCACCTATAAATTTATAGAAGTAATTGATTTCTGCCCTATGAGTTCTTAATCTTTAAAATATTTTCAAAGAAAAATTAAGTTAAAATATTATAAGTTAATCTTTGAAATTCAAAGACTAAATTTTTAAATTTTCATTCTGATGGATGTTTGGAGTAATGACTATCCTAAGATTATAGTAACCAACTCTTGTTACATTAAATACTATTTCTAAATCTCCATTTATTATATTATTTACTATTTGCTATAATTTTTATTATTTTACATTTATCATTTTTTTTATTCTTTGATATAGTGTTTATTATTTTCTTCTCAAACTAGTTCTAATTTTTTTCTTATGTGGCGCAGCGGGGTCCGGAACAGGAATAATTCTACGAGACATGATATTTGAGAACACAGCAGGACCAAGCAAGCACCAAGCGGTGGCATTACGCTTGAGCGCAGACCACGCCGTGGTATACCGATGCAACATCATTGGTTATCAAGACACTCTCTACGTGCACTCGAATCGCCAATTCTTCCGAGAGTGCGACATCTATGGTACAGTCGACTTCATCTTTGGGAACGCTGTAGTCGTTTTCCAAAGCTGCAACATCTATGCTCGAAAGCCAATGCCGGGACAAAAAAACACCATTACAGCCCAAGACAGGAAGGACCCAAACCAAAATACCGGAATCTCCATCCATGCTTGCAAGATAATTGCCACCAGCGACCTTCAAGCCTCCAAGGGTTCTTTCCCCACCTTCCTCGGCCGCCCTTGGAAGCTATACTCTAGAGTTGTGTACATGGTATCTTTCATGGGAGACCATATTCACCCACGAGGCTGGCTGGAGTGGCAAGGTTCTTTTGCTCTCGACACTCTTTACTATGGCGAGTATTCAAATTCTGGGCCCGGCGCTGCCGTGGGACAGCGGGTTAAGTGGTCCGGTTATCGAGTTATAACTTCCACGGTGGAAGCCTCTAAATTTACTGTTGGCCAATTTATTTATGGCTCTTCTTGGTTGCCCTCCACTGGAGTGTCATTTTCAGCTGGCTTATCTGCTAACTAATTAGAATGCATAATTTTTTTTTTTCATGAGTGTATTTAAATAATTAAAATTTGTTTTTTTCTTTTTTTTTTTTTATTTTTTATTTTTAGGATGTGGTTGATGATGACACGTGTATAGTCTCATTCCTTTGATTATGCTCTTGGAAAATAATATGAGCCACATCAAAATATGAGATGGTCATATATATAAATACATATATACATATATGGCTATAATTGTGATTGTATTTGTTGTATCAACATTATATATTTACTGAATAAATTTGTTTTTTTTCTCTCTATTCTCATGGATGTAAC

mRNA sequence

ATTTAAACTCATTCACTCCCATTATTCCCTCCTCAACCCCATGCACACTCATCTCATTATTCTCCTTCAACAACAACAACAACCATGATCAACAACAACTACAACAAGCTCCACTCCTCCCTCTCCGCCACCTCTACTCCGGCATCCTCCTTCCACCTCTCCGGCAATGATCTCCCGCCCACACCTCCACCCTCCTCCTCCCACCGCCGTCGCTCTCGTATCCTTATCATCTCCTTCCTCTCCGTCCTCCTCATCGCTGCCTCCATCGTCTCTGCCGCCGTCCTAGTCCGGTCCCGCATCGCGTCTAACGAACCCCATTTCCCACACAACAAGCCCTCCCAAGCCATCTCCCGCGCGTGTAGCCACACTCTCTACCCATCTCTCTGCCTCTCATCTCTTCTCTCCATCCCCGACGCGCTCTCCGCCGACGTTCATGACCTTGTTCATATCTCTCTCAACTTAACTCTTCAACATCTCACTAAGGCTCTTTATTCCACTTCTCAAATCCCTATCTCACAAATCTCTAACGACCCTTTAGCTCATTCAGCTTATGAAGACTGTATGGAGCTTCTAAATGACGCCGTTGACGCTTTTTCAGTTTCTCTTTTTTCTACTGAAGCTTCCAATCACGACGTCATGACGTGGCTTAGTGCGGCATTGACTTACCATGACACGTGTACGGATGGATTTGCGGATGTGGCTGACTTGGCTGTTAAGAATCAGGTGGAGGCGAAGTTGAGTGATTTGTCGGAGATGATCAGTAATTCTTTGGCTATATTTTCTGGGTTTGGTGGTGGGGATTTGCCAGTGCAGAATAGGCGGCGTCGGTTGATGGAGAGTGGTACGCGGTCATGGGCGGCGGAGAATGGTGGTGATGGGGGTTTTCCTCAATGGCTGAGTGTGAAGGATCGGCGGCTTTTGGCTGCTCCTATATCGGCGATTCAGGCGGATATTGTGGTGGCTAAGGATGGTTCTGGGAAGTTCAAGACGGTGGCTGAGGCCATTGACGCGGCGCCGTCGTCGAGTGGCCGCCGGATCGTCATCTACATTAAGGCCGGAAAGTACGAAGAGGAGAATTTGAAGGTGGGAAGGAAGAAAACAAACTTGATGTTTGTGGGAGATGGGAAGGGCATAACCGTCATTTCAGGTGGCAAAAGTGTTTTTGACAAAGTTACCACTTTTCGCACCGCCACCTTTGCGGGGTCCGGAACAGGAATAATTCTACGAGACATGATATTTGAGAACACAGCAGGACCAAGCAAGCACCAAGCGGTGGCATTACGCTTGAGCGCAGACCACGCCGTGGTATACCGATGCAACATCATTGGTTATCAAGACACTCTCTACGTGCACTCGAATCGCCAATTCTTCCGAGAGTGCGACATCTATGGTACAGTCGACTTCATCTTTGGGAACGCTGTAGTCGTTTTCCAAAGCTGCAACATCTATGCTCGAAAGCCAATGCCGGGACAAAAAAACACCATTACAGCCCAAGACAGGAAGGACCCAAACCAAAATACCGGAATCTCCATCCATGCTTGCAAGATAATTGCCACCAGCGACCTTCAAGCCTCCAAGGGTTCTTTCCCCACCTTCCTCGGCCGCCCTTGGAAGCTATACTCTAGAGTTGTGTACATGGTATCTTTCATGGGAGACCATATTCACCCACGAGGCTGGCTGGAGTGGCAAGGTTCTTTTGCTCTCGACACTCTTTACTATGGCGAGTATTCAAATTCTGGGCCCGGCGCTGCCGTGGGACAGCGGGTTAAGTGGTCCGGTTATCGAGTTATAACTTCCACGGTGGAAGCCTCTAAATTTACTGTTGGCCAATTTATTTATGGCTCTTCTTGGTTGCCCTCCACTGGAGTGTCATTTTCAGCTGGCTTATCTGCTAACTAATTAGAATGCATAATTTTTTTTTTTCATGAGTGTATTTAAATAATTAAAATTTGTTTTTTTCTTTTTTTTTTTTTATTTTTTATTTTTAGGATGTGGTTGATGATGACACGTGTATAGTCTCATTCCTTTGATTATGCTCTTGGAAAATAATATGAGCCACATCAAAATATGAGATGGTCATATATATAAATACATATATACATATATGGCTATAATTGTGATTGTATTTGTTGTATCAACATTATATATTTACTGAATAAATTTGTTTTTTTTCTCTCTATTCTCATGGATGTAAC

Coding sequence (CDS)

ATGATCAACAACAACTACAACAAGCTCCACTCCTCCCTCTCCGCCACCTCTACTCCGGCATCCTCCTTCCACCTCTCCGGCAATGATCTCCCGCCCACACCTCCACCCTCCTCCTCCCACCGCCGTCGCTCTCGTATCCTTATCATCTCCTTCCTCTCCGTCCTCCTCATCGCTGCCTCCATCGTCTCTGCCGCCGTCCTAGTCCGGTCCCGCATCGCGTCTAACGAACCCCATTTCCCACACAACAAGCCCTCCCAAGCCATCTCCCGCGCGTGTAGCCACACTCTCTACCCATCTCTCTGCCTCTCATCTCTTCTCTCCATCCCCGACGCGCTCTCCGCCGACGTTCATGACCTTGTTCATATCTCTCTCAACTTAACTCTTCAACATCTCACTAAGGCTCTTTATTCCACTTCTCAAATCCCTATCTCACAAATCTCTAACGACCCTTTAGCTCATTCAGCTTATGAAGACTGTATGGAGCTTCTAAATGACGCCGTTGACGCTTTTTCAGTTTCTCTTTTTTCTACTGAAGCTTCCAATCACGACGTCATGACGTGGCTTAGTGCGGCATTGACTTACCATGACACGTGTACGGATGGATTTGCGGATGTGGCTGACTTGGCTGTTAAGAATCAGGTGGAGGCGAAGTTGAGTGATTTGTCGGAGATGATCAGTAATTCTTTGGCTATATTTTCTGGGTTTGGTGGTGGGGATTTGCCAGTGCAGAATAGGCGGCGTCGGTTGATGGAGAGTGGTACGCGGTCATGGGCGGCGGAGAATGGTGGTGATGGGGGTTTTCCTCAATGGCTGAGTGTGAAGGATCGGCGGCTTTTGGCTGCTCCTATATCGGCGATTCAGGCGGATATTGTGGTGGCTAAGGATGGTTCTGGGAAGTTCAAGACGGTGGCTGAGGCCATTGACGCGGCGCCGTCGTCGAGTGGCCGCCGGATCGTCATCTACATTAAGGCCGGAAAGTACGAAGAGGAGAATTTGAAGGTGGGAAGGAAGAAAACAAACTTGATGTTTGTGGGAGATGGGAAGGGCATAACCGTCATTTCAGGTGGCAAAAGTGTTTTTGACAAAGTTACCACTTTTCGCACCGCCACCTTTGCGGGGTCCGGAACAGGAATAATTCTACGAGACATGATATTTGAGAACACAGCAGGACCAAGCAAGCACCAAGCGGTGGCATTACGCTTGAGCGCAGACCACGCCGTGGTATACCGATGCAACATCATTGGTTATCAAGACACTCTCTACGTGCACTCGAATCGCCAATTCTTCCGAGAGTGCGACATCTATGGTACAGTCGACTTCATCTTTGGGAACGCTGTAGTCGTTTTCCAAAGCTGCAACATCTATGCTCGAAAGCCAATGCCGGGACAAAAAAACACCATTACAGCCCAAGACAGGAAGGACCCAAACCAAAATACCGGAATCTCCATCCATGCTTGCAAGATAATTGCCACCAGCGACCTTCAAGCCTCCAAGGGTTCTTTCCCCACCTTCCTCGGCCGCCCTTGGAAGCTATACTCTAGAGTTGTGTACATGGTATCTTTCATGGGAGACCATATTCACCCACGAGGCTGGCTGGAGTGGCAAGGTTCTTTTGCTCTCGACACTCTTTACTATGGCGAGTATTCAAATTCTGGGCCCGGCGCTGCCGTGGGACAGCGGGTTAAGTGGTCCGGTTATCGAGTTATAACTTCCACGGTGGAAGCCTCTAAATTTACTGTTGGCCAATTTATTTATGGCTCTTCTTGGTTGCCCTCCACTGGAGTGTCATTTTCAGCTGGCTTATCTGCTAACTAA

Protein sequence

MINNNYNKLHSSLSATSTPASSFHLSGNDLPPTPPPSSSHRRRSRILIISFLSVLLIAASIVSAAVLVRSRIASNEPHFPHNKPSQAISRACSHTLYPSLCLSSLLSIPDALSADVHDLVHISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSVSLFSTEASNHDVMTWLSAALTYHDTCTDGFADVADLAVKNQVEAKLSDLSEMISNSLAIFSGFGGGDLPVQNRRRRLMESGTRSWAAENGGDGGFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAGSGTGIILRDMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKIIATSDLQASKGSFPTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFALDTLYYGEYSNSGPGAAVGQRVKWSGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLSAN
Homology
BLAST of Bhi12G000559 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 692.2 bits (1785), Expect = 3.7e-199
Identity = 360/581 (61.96%), Postives = 432/581 (74.35%), Query Frame = 0

Query: 31  PPTPPPSSSHRRRSRILIISFLSVLLIAASIVSAAVLVRSRIASNEPHFPHNKPSQAISR 90
           P T  P    + ++++++ + L+VL++          +R+  +         KP+QAISR
Sbjct: 19  PATSLPELQKKTKTKLILFT-LAVLVVGVVCFGIFAGIRAVDSGKTEPKLTRKPTQAISR 78

Query: 91  ACSHTLYPSLCLSSLLSIPDALSADVHDLVHISLNLTLQHLTKALYSTSQIPISQISNDP 150
            CS +LYP+LC+ +LL  P +L+AD ++L+HIS N TLQ  +KALY++S I  +Q+   P
Sbjct: 79  TCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSSTITYTQM--PP 138

Query: 151 LAHSAYEDCMELLNDAVDAF-----SVSLFSTEASNHDVMTWLSAALTYHDTCTDGFADV 210
              SAY+ C+ELL+D+VDA      SV + S + S+ DVMTWLS+A+T HDTCTDGF ++
Sbjct: 139 RVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTNHDTCTDGFDEI 198

Query: 211 ADLA--VKNQVEAKLSDLSEMISNSLAIFSG--FGGGDLPVQNRRRRLMESGTRSWAAEN 270
                 VK+QV   + DLSEM+SN LAIF+G       +PV N R+ L    T       
Sbjct: 199 EGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETEE----- 258

Query: 271 GGDGGFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIDAAPSSSGRRIVIY 330
                 P WL  +DR LL  P SAIQADI V+KDGSG FKT+AEAI  AP  S RR VIY
Sbjct: 259 -----LPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIY 318

Query: 331 IKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAGSGTGIILR 390
           +KAG+YEEENLKVGRKKTNLMF+GDGKG TVI+GGKS+ D +TTF TATFA +G G I+R
Sbjct: 319 VKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVR 378

Query: 391 DMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFI 450
           DM FEN AGP+KHQAVALR+  DHAVVYRCNIIGYQD LYVHSNRQFFREC+IYGTVDFI
Sbjct: 379 DMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFI 438

Query: 451 FGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKIIATSDLQASKGSF 510
           FGNA V+ QSCNIYARKPM  QK TITAQ+RKDPNQNTGISIHACK++AT DL+ASKGS+
Sbjct: 439 FGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSY 498

Query: 511 PTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFALDTLYYGEYSNSGPGAAVGQRV 570
           PT+LGRPWKLYSRVVYM+S MGDHI PRGWLEW G FALD+LYYGEY N G G+ +GQRV
Sbjct: 499 PTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRV 558

Query: 571 KWSGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 603
           KW GY VITSTVEASKFTV QFI GSSWLPSTGVSF +GLS
Sbjct: 559 KWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

BLAST of Bhi12G000559 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 684.9 bits (1766), Expect = 6.0e-197
Identity = 351/585 (60.00%), Postives = 438/585 (74.87%), Query Frame = 0

Query: 28  NDLPPTPPPSSSHRRRSRILIIS--FLSVLLIAASIVSAAVLVRSRIASNEPHFPHNKPS 87
           N +  +  P +++RR  + L++S   L++ LI A+ + A V  R ++  + P     KPS
Sbjct: 26  NQVSTSEQPENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLNQSVPGLA-RKPS 85

Query: 88  QAISRACSHTLYPSLCLSSLLSIPDALSA-DVHDLVHISLNLTLQHLTKALYSTSQIPIS 147
           QAIS+AC  T +P LC+ SL+  P +L+A    DL+H+++N+TL H + ALYS++   +S
Sbjct: 86  QAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSAS--LS 145

Query: 148 QISNDPLAHSAYEDCMELLNDAVDAFS---VSLFSTEASNHDVMTWLSAALTYHDTCTDG 207
            +   P A SAY+ C+ELL+D+VDA S    S+ S+ A   DV TWLSAALT HDTCT+G
Sbjct: 146 FVDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLSAALTNHDTCTEG 205

Query: 208 FADVADLAVKNQVEAKLSDLSEMISNSLAIFSGFGGGD----LPVQNRRRRLMESGTRSW 267
           F  V D  VK+ + A L +LSE++SN LAIFS    GD    +P+QNRR   +E      
Sbjct: 206 FDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLGVEEREEK- 265

Query: 268 AAENGGDGGFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIDAAPSSSGRR 327
                    FP+W+  K+R +L  P+S IQADI+V+KDG+G  KT++EAI  AP +S RR
Sbjct: 266 ---------FPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRR 325

Query: 328 IVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAGSGTG 387
           I+IY+KAG+YEE NLKVGRKK NLMFVGDGKG TVISGGKS+FD +TTF TA+FA +G G
Sbjct: 326 IIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGAG 385

Query: 388 IILRDMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGT 447
            I RD+ FEN AGP+KHQAVALR+ ADHAV+YRCNIIGYQDTLYVHSNRQFFRECDIYGT
Sbjct: 386 FIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGT 445

Query: 448 VDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKIIATSDLQAS 507
           VDFIFGNA VV Q+C+IYARKPM  QKNTITAQ+RKDPNQNTGISIHA +++A SDLQA+
Sbjct: 446 VDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQAT 505

Query: 508 KGSFPTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFALDTLYYGEYSNSGPGAAV 567
            GS  T+LGRPWKL+SR VYM+S++G H+H RGWLEW  +FALDTLYYGEY NSGPG+ +
Sbjct: 506 NGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGL 565

Query: 568 GQRVKWSGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 603
           GQRV W GYRVI ST EA++FTV +FIYGSSWLPSTGVSF AGLS
Sbjct: 566 GQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597

BLAST of Bhi12G000559 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 460.3 bits (1183), Expect = 2.4e-129
Identity = 258/584 (44.18%), Postives = 356/584 (60.96%), Query Frame = 0

Query: 41  RRRSRILIISFLSVLLIAASIVSAAVLVRSRIASNEPHFPHNKPSQAISRACSHTLYPSL 100
           ++  +++++S    LL  A++   A +      +NE           +  +CS T YP L
Sbjct: 15  KKNKKLVLLSAAVALLFVAAV---AGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPEL 74

Query: 101 CLSSLLSIPDALSADVHDLVHISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCM 160
           C+S++++          D++  S+NLT+  +    ++  ++   +    P   +A  DC+
Sbjct: 75  CISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCL 134

Query: 161 ELLNDAVDAF-----SVSLFSTEAS--NH--DVMTWLSAALTYHDTCTDGFA-DVADLAV 220
           E +++ +D        + L+ T+ +   H  D+ T +S+A+T  +TC DGF+ D AD  V
Sbjct: 135 ETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQV 194

Query: 221 KNQVEAKLSDLSEMISNSLAIFSGFGGGDLP--------VQNRRRRLMESGTRSWAAENG 280
           +  +      +  M SN+LA+       D+           N R+   E+   + A +  
Sbjct: 195 RKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAVDIA 254

Query: 281 GDG-----GFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIDAAPSSSGRR 340
           G G     G+P WLS  DRRLL    S ++AD  VA DGSG FKTVA A+ AAP +S +R
Sbjct: 255 GAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAAAVAAAPENSNKR 314

Query: 341 IVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAGSGTG 400
            VI+IKAG Y  EN++V +KK N+MF+GDG+  T+I+G ++V D  TTF +AT A  G  
Sbjct: 315 YVIHIKAGVY-RENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGER 374

Query: 401 IILRDMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGT 460
            + RD+ F+NTAGPSKHQAVALR+ +D +  Y C+++ YQDTLYVHSNRQFF +C I GT
Sbjct: 375 FLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGT 434

Query: 461 VDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKIIATSDLQAS 520
           VDFIFGNA VV Q C+I+AR+P  GQKN +TAQ R DPNQNTGI I  C+I ATSDLQ+ 
Sbjct: 435 VDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSV 494

Query: 521 KGSFPTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFALDTLYYGEYSNSGPGAAV 580
           KGSFPT+LGRPWK YS+ V M S + D I P GW EW G+FAL+TL Y EYSN+G GA  
Sbjct: 495 KGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGT 554

Query: 581 GQRVKWSGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
             RVKW G++VIT+  EA K+T GQFI G  WL STG  FS GL
Sbjct: 555 ANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of Bhi12G000559 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 459.1 bits (1180), Expect = 5.3e-129
Identity = 263/580 (45.34%), Postives = 347/580 (59.83%), Query Frame = 0

Query: 38  SSHRRRSRILIISFLSVLLIAASIVSAAVLVRSRIASNEPHFPHNKPSQAISRA-CSHTL 97
           S  +   ++++ S    LL+ ASIV  A    ++   N+     +  S AI ++ CS TL
Sbjct: 12  SDFKNNKKLILSSAAIALLLLASIVGIAATTTNQ-NKNQKITTLSSTSHAILKSVCSSTL 71

Query: 98  YPSLCLSSLLSIPDALSADVHDLVHISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAY 157
           YP LC S++ +          +++  SLNLT + +    ++  ++   +    P   +A 
Sbjct: 72  YPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTAL 131

Query: 158 EDCMELLNDAVDAFSVSL---------FSTEASNHDVMTWLSAALTYHDTCTDGFA-DVA 217
            DC+E +++ +D   V++          S      D+ T +S+A+T   TC DGF+ D A
Sbjct: 132 HDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDA 191

Query: 218 DLAVKNQVEAKLSDLSEMISNSLAIFSGFGGGDL---PVQNRRRRLMESGTRSWAAENG- 277
           D  V+  +      +  M SN+LA+       D+    ++++      +  R      G 
Sbjct: 192 DRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGD 251

Query: 278 -GDGGFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIDAAPSSSGRRIVIY 337
               G+P+WLSV DRRLL    S I+AD  VA DGSG F TVA A+ AAP  S +R VI+
Sbjct: 252 LDSDGWPKWLSVGDRRLLQG--STIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIH 311

Query: 338 IKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAGSGTGIILR 397
           IKAG Y  EN++V +KKTN+MF+GDG+G T+I+G ++V D  TTF +AT A  G   + R
Sbjct: 312 IKAGVY-RENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLAR 371

Query: 398 DMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFI 457
           D+ F+NTAGPSKHQAVALR+ +D +  Y+C++  YQDTLYVHSNRQFF +C I GTVDFI
Sbjct: 372 DITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFI 431

Query: 458 FGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKIIATSDLQASKGSF 517
           FGNA  V Q C+I AR+P  GQKN +TAQ R DPNQNTGI I  C+I  TSDL A KG+F
Sbjct: 432 FGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTF 491

Query: 518 PTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFALDTLYYGEYSNSGPGAAVGQRV 577
           PT+LGRPWK YSR V M S + D I P GW EW GSFALDTL Y EY N G GA    RV
Sbjct: 492 PTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRV 551

Query: 578 KWSGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
           KW GY+VITS  EA  FT GQFI G  WL STG  FS  L
Sbjct: 552 KWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of Bhi12G000559 vs. TAIR 10
Match: AT1G11580.1 (methylesterase PCR A )

HSP 1 Score: 424.5 bits (1090), Expect = 1.5e-118
Identity = 251/588 (42.69%), Postives = 347/588 (59.01%), Query Frame = 0

Query: 25  LSGNDLPPTPPPSSSHRRRSRILIISFLSVLLIAASIVSAAVLVRSRIASNEPHFPHNKP 84
           +S ++ P    P S  + ++  L++SF+++L   A   +  + V +          +N  
Sbjct: 1   MSNSNQPLLSKPKSL-KHKNLCLVLSFVAILGSVAFFTAQLISVNTN---------NNDD 60

Query: 85  SQAISRACSHTLYPSLCLSSLLSIPDALS---ADVHDLVHISLNLTLQHLTKALYSTSQI 144
           S   +    H  +      +LLS    LS    +  DL+H+ L  ++  L   +   S+ 
Sbjct: 61  SLLTTSQICHGAHDQDSCQALLSEFTTLSLSKLNRLDLLHVFLKNSVWRLESTMTMVSEA 120

Query: 145 PISQISNDPLAHSAYEDCMELLNDAVDAFSVSLFSTEASNH------DVMTWLSAALTYH 204
            I   SN     + + DC E+++ + D    S+      N+      +V TWLS+ LT +
Sbjct: 121 RIR--SNGVRDKAGFADCEEMMDVSKDRMMSSMEELRGGNYNLESYSNVHTWLSSVLTNY 180

Query: 205 DTCTDGFADVADLAVKNQVEAKLSDLSEMISNSLAIFSGFGGGDLPVQNRRRRLMESGTR 264
            TC +  +DV+ +  K  V+ +L DL      +LAIF       LP ++  + ++ +   
Sbjct: 181 MTCLESISDVS-VNSKQIVKPQLEDLVSRARVALAIFVSV----LPARDDLKMIISN--- 240

Query: 265 SWAAENGGDGGFPQWLSVKDRRLLAAPISAIQ--ADIVVAKDGSGKFKTVAEAIDAAPSS 324
                      FP WL+  DR+LL +    ++  A++VVAKDG+GKFKTV EA+ AAP +
Sbjct: 241 ----------RFPSWLTALDRKLLESSPKTLKVTANVVVAKDGTGKFKTVNEAVAAAPEN 300

Query: 325 SGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAG 384
           S  R VIY+K G Y +E + +G+KK NLM VGDGK  T+I+G  +V D  TTFR+AT A 
Sbjct: 301 SNTRYVIYVKKGVY-KETIDIGKKKKNLMLVGDGKDATIITGSLNVIDGSTTFRSATVAA 360

Query: 385 SGTGIILRDMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECD 444
           +G G + +D+ F+NTAGP+KHQAVALR+SAD  V+ RC I  YQDTLY H+ RQF+R+  
Sbjct: 361 NGDGFMAQDIWFQNTAGPAKHQAVALRVSADQTVINRCRIDAYQDTLYTHTLRQFYRDSY 420

Query: 445 IYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKIIATSD 504
           I GTVDFIFGN+ VVFQ+C+I AR P  GQKN +TAQ R+D NQNT ISI  CKI A+SD
Sbjct: 421 ITGTVDFIFGNSAVVFQNCDIVARNPGAGQKNMLTAQGREDQNQNTAISIQKCKITASSD 480

Query: 505 LQASKGSFPTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFALDTLYYGEYSNSGP 564
           L   KGS  TFLGRPWKLYSR V M SF+ +HI P GW  W G FAL TLYYGEY+N+GP
Sbjct: 481 LAPVKGSVKTFLGRPWKLYSRTVIMQSFIDNHIDPAGWFPWDGEFALSTLYYGEYANTGP 540

Query: 565 GAAVGQRVKWSGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
           GA   +RV W G++VI  + EA +FTV + I G  WL  TGV+F   L
Sbjct: 541 GADTSKRVNWKGFKVIKDSKEAEQFTVAKLIQGGLWLKPTGVTFQEWL 557

BLAST of Bhi12G000559 vs. ExPASy Swiss-Prot
Match: Q9FK05 (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3702 GN=PME61 PE=2 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 5.3e-198
Identity = 360/581 (61.96%), Postives = 432/581 (74.35%), Query Frame = 0

Query: 31  PPTPPPSSSHRRRSRILIISFLSVLLIAASIVSAAVLVRSRIASNEPHFPHNKPSQAISR 90
           P T  P    + ++++++ + L+VL++          +R+  +         KP+QAISR
Sbjct: 19  PATSLPELQKKTKTKLILFT-LAVLVVGVVCFGIFAGIRAVDSGKTEPKLTRKPTQAISR 78

Query: 91  ACSHTLYPSLCLSSLLSIPDALSADVHDLVHISLNLTLQHLTKALYSTSQIPISQISNDP 150
            CS +LYP+LC+ +LL  P +L+AD ++L+HIS N TLQ  +KALY++S I  +Q+   P
Sbjct: 79  TCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSSTITYTQM--PP 138

Query: 151 LAHSAYEDCMELLNDAVDAF-----SVSLFSTEASNHDVMTWLSAALTYHDTCTDGFADV 210
              SAY+ C+ELL+D+VDA      SV + S + S+ DVMTWLS+A+T HDTCTDGF ++
Sbjct: 139 RVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTNHDTCTDGFDEI 198

Query: 211 ADLA--VKNQVEAKLSDLSEMISNSLAIFSG--FGGGDLPVQNRRRRLMESGTRSWAAEN 270
                 VK+QV   + DLSEM+SN LAIF+G       +PV N R+ L    T       
Sbjct: 199 EGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETEE----- 258

Query: 271 GGDGGFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIDAAPSSSGRRIVIY 330
                 P WL  +DR LL  P SAIQADI V+KDGSG FKT+AEAI  AP  S RR VIY
Sbjct: 259 -----LPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIY 318

Query: 331 IKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAGSGTGIILR 390
           +KAG+YEEENLKVGRKKTNLMF+GDGKG TVI+GGKS+ D +TTF TATFA +G G I+R
Sbjct: 319 VKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVR 378

Query: 391 DMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFI 450
           DM FEN AGP+KHQAVALR+  DHAVVYRCNIIGYQD LYVHSNRQFFREC+IYGTVDFI
Sbjct: 379 DMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFI 438

Query: 451 FGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKIIATSDLQASKGSF 510
           FGNA V+ QSCNIYARKPM  QK TITAQ+RKDPNQNTGISIHACK++AT DL+ASKGS+
Sbjct: 439 FGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSY 498

Query: 511 PTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFALDTLYYGEYSNSGPGAAVGQRV 570
           PT+LGRPWKLYSRVVYM+S MGDHI PRGWLEW G FALD+LYYGEY N G G+ +GQRV
Sbjct: 499 PTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRV 558

Query: 571 KWSGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 603
           KW GY VITSTVEASKFTV QFI GSSWLPSTGVSF +GLS
Sbjct: 559 KWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

BLAST of Bhi12G000559 vs. ExPASy Swiss-Prot
Match: Q9M3B0 (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3702 GN=PME34 PE=2 SV=1)

HSP 1 Score: 684.9 bits (1766), Expect = 8.4e-196
Identity = 351/585 (60.00%), Postives = 438/585 (74.87%), Query Frame = 0

Query: 28  NDLPPTPPPSSSHRRRSRILIIS--FLSVLLIAASIVSAAVLVRSRIASNEPHFPHNKPS 87
           N +  +  P +++RR  + L++S   L++ LI A+ + A V  R ++  + P     KPS
Sbjct: 26  NQVSTSEQPENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLNQSVPGLA-RKPS 85

Query: 88  QAISRACSHTLYPSLCLSSLLSIPDALSA-DVHDLVHISLNLTLQHLTKALYSTSQIPIS 147
           QAIS+AC  T +P LC+ SL+  P +L+A    DL+H+++N+TL H + ALYS++   +S
Sbjct: 86  QAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSAS--LS 145

Query: 148 QISNDPLAHSAYEDCMELLNDAVDAFS---VSLFSTEASNHDVMTWLSAALTYHDTCTDG 207
            +   P A SAY+ C+ELL+D+VDA S    S+ S+ A   DV TWLSAALT HDTCT+G
Sbjct: 146 FVDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLSAALTNHDTCTEG 205

Query: 208 FADVADLAVKNQVEAKLSDLSEMISNSLAIFSGFGGGD----LPVQNRRRRLMESGTRSW 267
           F  V D  VK+ + A L +LSE++SN LAIFS    GD    +P+QNRR   +E      
Sbjct: 206 FDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLGVEEREEK- 265

Query: 268 AAENGGDGGFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIDAAPSSSGRR 327
                    FP+W+  K+R +L  P+S IQADI+V+KDG+G  KT++EAI  AP +S RR
Sbjct: 266 ---------FPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRR 325

Query: 328 IVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAGSGTG 387
           I+IY+KAG+YEE NLKVGRKK NLMFVGDGKG TVISGGKS+FD +TTF TA+FA +G G
Sbjct: 326 IIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGAG 385

Query: 388 IILRDMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGT 447
            I RD+ FEN AGP+KHQAVALR+ ADHAV+YRCNIIGYQDTLYVHSNRQFFRECDIYGT
Sbjct: 386 FIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGT 445

Query: 448 VDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKIIATSDLQAS 507
           VDFIFGNA VV Q+C+IYARKPM  QKNTITAQ+RKDPNQNTGISIHA +++A SDLQA+
Sbjct: 446 VDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQAT 505

Query: 508 KGSFPTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFALDTLYYGEYSNSGPGAAV 567
            GS  T+LGRPWKL+SR VYM+S++G H+H RGWLEW  +FALDTLYYGEY NSGPG+ +
Sbjct: 506 NGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGL 565

Query: 568 GQRVKWSGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 603
           GQRV W GYRVI ST EA++FTV +FIYGSSWLPSTGVSF AGLS
Sbjct: 566 GQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597

BLAST of Bhi12G000559 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 3.4e-128
Identity = 261/573 (45.55%), Postives = 355/573 (61.95%), Query Frame = 0

Query: 42  RRSRILIISFLSVLLIAASIVSAAVLVRSRIASNEPHFPHNKPSQAISR-ACSHTLYPSL 101
           ++ + L ++  + LL+ A+++     V SR  S +     N+P  AI + +CS T YP L
Sbjct: 25  KKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGD---NGNEPHHAILKSSCSSTRYPDL 84

Query: 102 CLSSLLSIPDALS--ADVHDLVHISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYED 161
           C S++ ++P+A        D++ +SLN+T   +    +   ++ + + +       A  D
Sbjct: 85  CFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKL-LKRTNLTKREKVALHD 144

Query: 162 CMELLNDAVDAFSVSLFSTE-------ASNH--DVMTWLSAALTYHDTCTDGFA-DVADL 221
           C+E +++ +D    ++   E        S H  D+ T +SAA+T   TC DGF+ D A+ 
Sbjct: 145 CLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANK 204

Query: 222 AVKNQVEAKLSDLSEMISNSLAIFSGFGGGDLPVQNRRRRLMESGTRSWAAENGGDGGFP 281
            V++ +      + +M SN+LA+       D+ +      +  S  R    E     G+P
Sbjct: 205 HVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMI------MRTSNNRKLTEETSTVDGWP 264

Query: 282 QWLSVKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIDAAPSSSGRRIVIYIKAGKYE 341
            WLS  DRRLL +  S++  + VVA DGSG FKTVA A+ AAP    +R +I IKAG Y 
Sbjct: 265 AWLSPGDRRLLQS--SSVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKRYIIRIKAGVY- 324

Query: 342 EENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAGSGTGIILRDMIFENT 401
            EN++V +K  N+MF+GDG+  T+I+G ++V D  TTF++AT A  G G + RD+ F+NT
Sbjct: 325 RENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGEGFLARDITFQNT 384

Query: 402 AGPSKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAVVV 461
           AGPSKHQAVALR+ AD +  Y C+++ YQDTLYVHSNRQFF  C I GTVDFIFGNA  V
Sbjct: 385 AGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAV 444

Query: 462 FQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKIIATSDLQASKGSFPTFLGRP 521
            Q+C+I+ARKP  GQKN +TAQ R DPNQNTGI I   +I ATSDL+  +GSFPT+LGRP
Sbjct: 445 LQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRP 504

Query: 522 WKLYSRVVYMVSFMGDHIHPRGWLEWQGSFALDTLYYGEYSNSGPGAAVGQRVKWSGYRV 581
           WK YSR V M S + D IHP GW EW G+FAL+TL+YGE+ NSG GA    RVKW G+RV
Sbjct: 505 WKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRV 564

Query: 582 ITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
           ITS  EA  FT G FI GSSWL STG  FS GL
Sbjct: 565 ITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Bhi12G000559 vs. ExPASy Swiss-Prot
Match: O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)

HSP 1 Score: 460.3 bits (1183), Expect = 3.4e-128
Identity = 258/584 (44.18%), Postives = 356/584 (60.96%), Query Frame = 0

Query: 41  RRRSRILIISFLSVLLIAASIVSAAVLVRSRIASNEPHFPHNKPSQAISRACSHTLYPSL 100
           ++  +++++S    LL  A++   A +      +NE           +  +CS T YP L
Sbjct: 15  KKNKKLVLLSAAVALLFVAAV---AGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPEL 74

Query: 101 CLSSLLSIPDALSADVHDLVHISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCM 160
           C+S++++          D++  S+NLT+  +    ++  ++   +    P   +A  DC+
Sbjct: 75  CISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCL 134

Query: 161 ELLNDAVDAF-----SVSLFSTEAS--NH--DVMTWLSAALTYHDTCTDGFA-DVADLAV 220
           E +++ +D        + L+ T+ +   H  D+ T +S+A+T  +TC DGF+ D AD  V
Sbjct: 135 ETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQV 194

Query: 221 KNQVEAKLSDLSEMISNSLAIFSGFGGGDLP--------VQNRRRRLMESGTRSWAAENG 280
           +  +      +  M SN+LA+       D+           N R+   E+   + A +  
Sbjct: 195 RKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAVDIA 254

Query: 281 GDG-----GFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIDAAPSSSGRR 340
           G G     G+P WLS  DRRLL    S ++AD  VA DGSG FKTVA A+ AAP +S +R
Sbjct: 255 GAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAAAVAAAPENSNKR 314

Query: 341 IVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAGSGTG 400
            VI+IKAG Y  EN++V +KK N+MF+GDG+  T+I+G ++V D  TTF +AT A  G  
Sbjct: 315 YVIHIKAGVY-RENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGER 374

Query: 401 IILRDMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGT 460
            + RD+ F+NTAGPSKHQAVALR+ +D +  Y C+++ YQDTLYVHSNRQFF +C I GT
Sbjct: 375 FLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGT 434

Query: 461 VDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKIIATSDLQAS 520
           VDFIFGNA VV Q C+I+AR+P  GQKN +TAQ R DPNQNTGI I  C+I ATSDLQ+ 
Sbjct: 435 VDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSV 494

Query: 521 KGSFPTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFALDTLYYGEYSNSGPGAAV 580
           KGSFPT+LGRPWK YS+ V M S + D I P GW EW G+FAL+TL Y EYSN+G GA  
Sbjct: 495 KGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGT 554

Query: 581 GQRVKWSGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
             RVKW G++VIT+  EA K+T GQFI G  WL STG  FS GL
Sbjct: 555 ANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of Bhi12G000559 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 3.4e-128
Identity = 259/573 (45.20%), Postives = 356/573 (62.13%), Query Frame = 0

Query: 42  RRSRILIISFLSVLLIAASIVSAAVLVRSRIASNEPHFPHNKPSQAISR-ACSHTLYPSL 101
           ++ + L ++  + LL+ A+++     V SR  S +     N+P  AI + +CS T YP L
Sbjct: 25  KKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGD---NGNEPHHAILKSSCSSTRYPDL 84

Query: 102 CLSSLLSIPDALS--ADVHDLVHISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYED 161
           C S++ ++P+A        D++ +SLN+T   +    +   ++ + + +       A  D
Sbjct: 85  CFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKL-LKRTNLTKREKVALHD 144

Query: 162 CMELLNDAVDAFSVSLFSTE-------ASNH--DVMTWLSAALTYHDTCTDGFA-DVADL 221
           C+E +++ +D    ++   E        S H  D+ T +SAA+T   TC DGF+ D A+ 
Sbjct: 145 CLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANK 204

Query: 222 AVKNQVEAKLSDLSEMISNSLAIFSGFGGGDLPVQNRRRRLMESGTRSWAAENGGDGGFP 281
            V++ +      + +M SN+LA+       D+ +      +  S  R    E     G+P
Sbjct: 205 HVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMI------MRTSNNRKLIEETSTVDGWP 264

Query: 282 QWLSVKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIDAAPSSSGRRIVIYIKAGKYE 341
            WLS  DRRLL +  S++  ++VVA DGSG FKTVA ++ AAP    +R +I IKAG Y 
Sbjct: 265 AWLSTGDRRLLQS--SSVTPNVVVAADGSGNFKTVAASVAAAPQGGTKRYIIRIKAGVY- 324

Query: 342 EENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAGSGTGIILRDMIFENT 401
            EN++V +K  N+MF+GDG+  T+I+G ++V D  TTF++AT A  G G + RD+ F+NT
Sbjct: 325 RENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARDITFQNT 384

Query: 402 AGPSKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAVVV 461
           AGPSKHQAVALR+ AD +  Y C+++ YQDTLYVHSNRQFF  C I GTVDFIFGNA  V
Sbjct: 385 AGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAV 444

Query: 462 FQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKIIATSDLQASKGSFPTFLGRP 521
            Q+C+I+ARKP  GQKN +TAQ R DPNQNTGI I   +I ATSDL+  +GSFPT+LGRP
Sbjct: 445 LQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRP 504

Query: 522 WKLYSRVVYMVSFMGDHIHPRGWLEWQGSFALDTLYYGEYSNSGPGAAVGQRVKWSGYRV 581
           WK YSR V M S + D IHP GW EW G+FAL+TL+YGE+ N+G GA    RVKW G+RV
Sbjct: 505 WKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRV 564

Query: 582 ITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
           ITS  EA  FT G FI GSSWL STG  FS GL
Sbjct: 565 ITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Bhi12G000559 vs. NCBI nr
Match: XP_038874841.1 (probable pectinesterase/pectinesterase inhibitor 61 [Benincasa hispida])

HSP 1 Score: 1191.4 bits (3081), Expect = 0.0e+00
Identity = 604/604 (100.00%), Postives = 604/604 (100.00%), Query Frame = 0

Query: 1   MINNNYNKLHSSLSATSTPASSFHLSGNDLPPTPPPSSSHRRRSRILIISFLSVLLIAAS 60
           MINNNYNKLHSSLSATSTPASSFHLSGNDLPPTPPPSSSHRRRSRILIISFLSVLLIAAS
Sbjct: 1   MINNNYNKLHSSLSATSTPASSFHLSGNDLPPTPPPSSSHRRRSRILIISFLSVLLIAAS 60

Query: 61  IVSAAVLVRSRIASNEPHFPHNKPSQAISRACSHTLYPSLCLSSLLSIPDALSADVHDLV 120
           IVSAAVLVRSRIASNEPHFPHNKPSQAISRACSHTLYPSLCLSSLLSIPDALSADVHDLV
Sbjct: 61  IVSAAVLVRSRIASNEPHFPHNKPSQAISRACSHTLYPSLCLSSLLSIPDALSADVHDLV 120

Query: 121 HISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSVSLFSTEAS 180
           HISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSVSLFSTEAS
Sbjct: 121 HISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSVSLFSTEAS 180

Query: 181 NHDVMTWLSAALTYHDTCTDGFADVADLAVKNQVEAKLSDLSEMISNSLAIFSGFGGGDL 240
           NHDVMTWLSAALTYHDTCTDGFADVADLAVKNQVEAKLSDLSEMISNSLAIFSGFGGGDL
Sbjct: 181 NHDVMTWLSAALTYHDTCTDGFADVADLAVKNQVEAKLSDLSEMISNSLAIFSGFGGGDL 240

Query: 241 PVQNRRRRLMESGTRSWAAENGGDGGFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGKF 300
           PVQNRRRRLMESGTRSWAAENGGDGGFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGKF
Sbjct: 241 PVQNRRRRLMESGTRSWAAENGGDGGFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGKF 300

Query: 301 KTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVF 360
           KTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVF
Sbjct: 301 KTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVF 360

Query: 361 DKVTTFRTATFAGSGTGIILRDMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTL 420
           DKVTTFRTATFAGSGTGIILRDMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTL
Sbjct: 361 DKVTTFRTATFAGSGTGIILRDMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTL 420

Query: 421 YVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTG 480
           YVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTG
Sbjct: 421 YVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTG 480

Query: 481 ISIHACKIIATSDLQASKGSFPTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFAL 540
           ISIHACKIIATSDLQASKGSFPTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFAL
Sbjct: 481 ISIHACKIIATSDLQASKGSFPTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFAL 540

Query: 541 DTLYYGEYSNSGPGAAVGQRVKWSGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 600
           DTLYYGEYSNSGPGAAVGQRVKWSGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG
Sbjct: 541 DTLYYGEYSNSGPGAAVGQRVKWSGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 600

Query: 601 LSAN 605
           LSAN
Sbjct: 601 LSAN 604

BLAST of Bhi12G000559 vs. NCBI nr
Match: XP_008437596.1 (PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor 61 [Cucumis melo])

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 551/605 (91.07%), Postives = 572/605 (94.55%), Query Frame = 0

Query: 3   NNNYNKLHSSLSATSTPASSFHLSGNDLPPTPPPSSSHRRRSRILIISFLSVLLIAASIV 62
           NNNY +LH+S SA +TPA+SF LSGND    PPPSSS RRRSRILIISF+SVLLIAAS+V
Sbjct: 4   NNNYKQLHTSHSANTTPATSFRLSGND---PPPPSSSLRRRSRILIISFMSVLLIAASVV 63

Query: 63  SAAVLVRSRIASNEPHFPHNKPSQAISRACSHTLYPSLCLSSLLSIPDALSADVHDLVHI 122
           SAA+LVRSRIAS++PHFPHNKPSQAISRACSHTLYPSLCLSSLLS P A SADVHDLVHI
Sbjct: 64  SAAILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 123

Query: 123 SLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSVSLFSTEASNH 182
           SLNLTLQHLTKALYSTSQIPI QIS DPLAHSAYEDCMELLNDA+DAFS+SLFST ASNH
Sbjct: 124 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNH 183

Query: 183 DVMTWLSAALTYHDTCTDGFADVADLAVKNQVEAKLSDLSEMISNSLAIFSGFGGGDLPV 242
           D+MTWLSAALTYHDTCTDGF DV DL VKN+VEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 184 DIMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 243

Query: 243 QNR-RRRLMESGT-RSWAAENGGD-GGFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGK 302
           +NR RRRLMESGT  SWAAENGGD  GFP+WLS KDRRLLAAPISAIQADIVVAKDGSGK
Sbjct: 244 ENRKRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGK 303

Query: 303 FKTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV 362
           FKTVAEAI+AAPSSSGRRI+IYIKAGKYEEE LKVGRKKTNLMFVGDGKGITVISGGKSV
Sbjct: 304 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSV 363

Query: 363 FDKVTTFRTATFAGSGTGIILRDMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 422
           +DKVTTFRTATFAGSGTGIILRDM FENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT
Sbjct: 364 YDKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 423

Query: 423 LYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNT 482
           LYVHSNRQFFRECDIYGT+DFIFGNAVVVFQSCNIYARKPM GQK TITAQDRKDPNQNT
Sbjct: 424 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKXTITAQDRKDPNQNT 483

Query: 483 GISIHACKIIATSDLQASKGSFPTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFA 542
           GISIHACKI+AT DL+ASKGSFPTFLGRPWKLYSRVVYMVS MGDHIHPRGWLEWQGSFA
Sbjct: 484 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 543

Query: 543 LDTLYYGEYSNSGPGAAVGQRVKWSGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 602
           LDTLYYGEY NSGPGAAVGQRVKW GYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA
Sbjct: 544 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 603

Query: 603 GLSAN 605
           GLS N
Sbjct: 604 GLSTN 605

BLAST of Bhi12G000559 vs. NCBI nr
Match: XP_011654619.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucumis sativus] >KGN49865.1 hypothetical protein Csa_000423 [Cucumis sativus])

HSP 1 Score: 1070.5 bits (2767), Expect = 5.6e-309
Identity = 543/604 (89.90%), Postives = 568/604 (94.04%), Query Frame = 0

Query: 3   NNNYNKLHSSLSATSTPASSFHLSGNDLPPTPPPSSSHRRRSRILIISFLSVLLIAASIV 62
           NN+YNKLH+S SA +TPA+SF LSGND    PPPSSS RRRSRILIISF+SVLLIAAS+V
Sbjct: 4   NNSYNKLHTSHSANTTPAASFRLSGND---PPPPSSSLRRRSRILIISFMSVLLIAASVV 63

Query: 63  SAAVLVRSRIASNEPHFPHNKPSQAISRACSHTLYPSLCLSSLLSIPDALSADVHDLVHI 122
           SAA+LVRS + S++PHFPHNKPSQAISRACSHTLYPSLCLSSLLS P A SADVHDLVHI
Sbjct: 64  SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 123

Query: 123 SLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSVSLFSTEASNH 182
           SLNLTLQHLTKALYSTSQIP+ QIS DPLAHSAYEDCMELLNDA+DAFS+SLFS +ASNH
Sbjct: 124 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 183

Query: 183 DVMTWLSAALTYHDTCTDGFADVADLAVKNQVEAKLSDLSEMISNSLAIFSGFGGGDLPV 242
           D+MTWLSAALTYHDTCT GF DVADL VK++VEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 184 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 243

Query: 243 QNR-RRRLMESGTRSWAAENGGD-GGFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGKF 302
           +NR RRRLMES T SWAAENGGD  GFP WLS KDRRLLAAP+S IQADIVVAKDGSGKF
Sbjct: 244 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 303

Query: 303 KTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVF 362
           KTVAEAI+AAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV+
Sbjct: 304 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 363

Query: 363 DKVTTFRTATFAGSGTGIILRDMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTL 422
           DKVTTFRTATFAGSGT IILRDM FENTAGPSKHQAVALRLSADHAVVY CNIIGYQDTL
Sbjct: 364 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 423

Query: 423 YVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTG 482
           YVHSNRQFFRECDIYGT+DFIFGNAVVVFQSCNIYARKPM GQKNTITAQDRKDPNQNTG
Sbjct: 424 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 483

Query: 483 ISIHACKIIATSDLQASKGSFPTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFAL 542
           ISIHACKI+AT DL+ASKGSFPTFLGRPWKLYSRVVYMVS MGDHIHPRGWLEWQGSFAL
Sbjct: 484 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 543

Query: 543 DTLYYGEYSNSGPGAAVGQRVKWSGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 602
           DTLYYGEY NSGPGAAVGQRVKW GYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG
Sbjct: 544 DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 603

Query: 603 LSAN 605
           LS N
Sbjct: 604 LSTN 604

BLAST of Bhi12G000559 vs. NCBI nr
Match: KAG6579321.1 (putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1021.5 bits (2640), Expect = 2.9e-294
Identity = 527/605 (87.11%), Postives = 554/605 (91.57%), Query Frame = 0

Query: 2   INNNYNKLHSSLSATSTPASSFHLSGNDLPPTPPPSSSHRRRSRILIISFLSVLLIAASI 61
           +NNNYNKLHSS S   TPASSF +S +   P PPPS S  RR RIL+ISFLS+ LI AS 
Sbjct: 1   MNNNYNKLHSSRSG-DTPASSFRVSNDHSDPLPPPSPSSSRR-RILLISFLSIFLITASA 60

Query: 62  VSAAVLVRSRI-ASNEPHFPHNKPSQAISRACSHTLYPSLCLSSLLSIPDALSADVHDLV 121
           VSA VLV SRI AS E HFPHNKP+QAISRACS TLYPSLCLSSLLS P +LSADVHDLV
Sbjct: 61  VSAVVLVHSRIAASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLV 120

Query: 122 HISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSVSLFSTEAS 181
           HISLNLTLQHLTKALYSTSQIPISQISNDPLA SAYEDCMELLNDAV+AFS+SLFST A+
Sbjct: 121 HISLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTAT 180

Query: 182 NHDVMTWLSAALTYHDTCTDGFADVADLAVKNQVEAKLSDLSEMISNSLAIFSGFGGGDL 241
           NHDVMTWLSAALTYHDTCTDGF+DVAD  +K+Q+ AKLSDLSEMISNSLAIFSGFGG DL
Sbjct: 181 NHDVMTWLSAALTYHDTCTDGFSDVAD-PIKDQITAKLSDLSEMISNSLAIFSGFGGDDL 240

Query: 242 PVQN-RRRRLMESGTRSWAAENGGDGGFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGK 301
           PV+N RRRRLMES     A EN GDG FPQW+S KDRRLLAAPIS+IQADIVVAKDGSGK
Sbjct: 241 PVENRRRRRLMESDEHWLAEENNGDGDFPQWVSGKDRRLLAAPISSIQADIVVAKDGSGK 300

Query: 302 FKTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV 361
           FKTVAEAI AAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV
Sbjct: 301 FKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV 360

Query: 362 FDKVTTFRTATFAGSGTGIILRDMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 421
           +DKVTTFRTATFAGSGTG+ILRDM FENTAGP+KHQAVALRLSADHAVV+R N+IGYQDT
Sbjct: 361 YDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQDT 420

Query: 422 LYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNT 481
           LYVHSNRQFFRECDIYGT+DFIFGNA VVFQSCNIYARKPM GQKNTITAQDRKDPNQNT
Sbjct: 421 LYVHSNRQFFRECDIYGTIDFIFGNAAVVFQSCNIYARKPMSGQKNTITAQDRKDPNQNT 480

Query: 482 GISIHACKIIATSDLQASKGSFPTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFA 541
           GISIHACKIIAT DL+ASKGSFPT+LGRPWKLYSRVVYMVS MGDHIHPRGWLEWQGSFA
Sbjct: 481 GISIHACKIIATGDLEASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540

Query: 542 LDTLYYGEYSNSGPGAAVGQRVKWSGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 601
           LDTLYYGEY NSGPGAAVGQRVKW GYRVITST+EA+KFTVGQFIYGSSWLPSTGVSFSA
Sbjct: 541 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSA 600

Query: 602 GLSAN 605
           GLS N
Sbjct: 601 GLSTN 602

BLAST of Bhi12G000559 vs. NCBI nr
Match: XP_022922302.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita moschata])

HSP 1 Score: 1018.5 bits (2632), Expect = 2.4e-293
Identity = 526/605 (86.94%), Postives = 554/605 (91.57%), Query Frame = 0

Query: 2   INNNYNKLHSSLSATSTPASSFHLSGNDLPPTPPPSSSHRRRSRILIISFLSVLLIAASI 61
           +NNNYN LHSS S   TPASSF +S +   P PPPS S  RR RIL+ISF S+LLI AS 
Sbjct: 1   MNNNYNMLHSSHSG-DTPASSFRVSNDHSDPLPPPSPSSSRR-RILLISFFSILLITASA 60

Query: 62  VSAAVLVRSRI-ASNEPHFPHNKPSQAISRACSHTLYPSLCLSSLLSIPDALSADVHDLV 121
           VSA VLV SRI AS E HFPHNKP+QAISRACS TLYPSLCLSSLLS P +LSADVHDLV
Sbjct: 61  VSAVVLVHSRIAASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLV 120

Query: 122 HISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSVSLFSTEAS 181
           HISLNLTLQHLTKALYSTSQIPISQISNDPLA SAYEDCMELLNDAV+AFS+SLFST A+
Sbjct: 121 HISLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTAT 180

Query: 182 NHDVMTWLSAALTYHDTCTDGFADVADLAVKNQVEAKLSDLSEMISNSLAIFSGFGGGDL 241
           NHDVMTWLSAALTYHDTCTDGF+DVAD  +K+Q+ AKLSDLSEMISNSLAIFSGFGG DL
Sbjct: 181 NHDVMTWLSAALTYHDTCTDGFSDVAD-PIKDQITAKLSDLSEMISNSLAIFSGFGGDDL 240

Query: 242 PVQN-RRRRLMESGTRSWAAENGGDGGFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGK 301
           PV+N RRRRLMES  +  A EN GDG FP+W+S KDRRLLAAPIS+IQADIVVAKDGSGK
Sbjct: 241 PVENRRRRRLMESDEQWLAEENNGDGDFPRWVSGKDRRLLAAPISSIQADIVVAKDGSGK 300

Query: 302 FKTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV 361
           FKTVAEAI AAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV
Sbjct: 301 FKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV 360

Query: 362 FDKVTTFRTATFAGSGTGIILRDMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 421
           +DKVTTFRTATFAGSGTG+ILRDM FENTAGP+KHQAVALRLSADHAVV+R N+IGYQDT
Sbjct: 361 YDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQDT 420

Query: 422 LYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNT 481
           LYVHSNRQFFRECDIYGT+DFIFGNAVVVFQSCNIYARKPM GQKNTITAQDRKDPNQNT
Sbjct: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMSGQKNTITAQDRKDPNQNT 480

Query: 482 GISIHACKIIATSDLQASKGSFPTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFA 541
           GISIHACKIIAT DLQASKGSFPT+LGRPWKLYSRVVYMVS MGDHIHPRGWLEWQGSFA
Sbjct: 481 GISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540

Query: 542 LDTLYYGEYSNSGPGAAVGQRVKWSGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 601
           LDTLYYGEY NSGPGAAVGQRVKW GYRVITS +EA+KFTVGQFIYGSSWLPSTGVSFSA
Sbjct: 541 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSAIEATKFTVGQFIYGSSWLPSTGVSFSA 600

Query: 602 GLSAN 605
           GLS N
Sbjct: 601 GLSTN 602

BLAST of Bhi12G000559 vs. ExPASy TrEMBL
Match: A0A1S3AUZ6 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482964 PE=3 SV=1)

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 551/605 (91.07%), Postives = 572/605 (94.55%), Query Frame = 0

Query: 3   NNNYNKLHSSLSATSTPASSFHLSGNDLPPTPPPSSSHRRRSRILIISFLSVLLIAASIV 62
           NNNY +LH+S SA +TPA+SF LSGND    PPPSSS RRRSRILIISF+SVLLIAAS+V
Sbjct: 4   NNNYKQLHTSHSANTTPATSFRLSGND---PPPPSSSLRRRSRILIISFMSVLLIAASVV 63

Query: 63  SAAVLVRSRIASNEPHFPHNKPSQAISRACSHTLYPSLCLSSLLSIPDALSADVHDLVHI 122
           SAA+LVRSRIAS++PHFPHNKPSQAISRACSHTLYPSLCLSSLLS P A SADVHDLVHI
Sbjct: 64  SAAILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 123

Query: 123 SLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSVSLFSTEASNH 182
           SLNLTLQHLTKALYSTSQIPI QIS DPLAHSAYEDCMELLNDA+DAFS+SLFST ASNH
Sbjct: 124 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNH 183

Query: 183 DVMTWLSAALTYHDTCTDGFADVADLAVKNQVEAKLSDLSEMISNSLAIFSGFGGGDLPV 242
           D+MTWLSAALTYHDTCTDGF DV DL VKN+VEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 184 DIMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 243

Query: 243 QNR-RRRLMESGT-RSWAAENGGD-GGFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGK 302
           +NR RRRLMESGT  SWAAENGGD  GFP+WLS KDRRLLAAPISAIQADIVVAKDGSGK
Sbjct: 244 ENRKRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGK 303

Query: 303 FKTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV 362
           FKTVAEAI+AAPSSSGRRI+IYIKAGKYEEE LKVGRKKTNLMFVGDGKGITVISGGKSV
Sbjct: 304 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSV 363

Query: 363 FDKVTTFRTATFAGSGTGIILRDMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 422
           +DKVTTFRTATFAGSGTGIILRDM FENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT
Sbjct: 364 YDKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 423

Query: 423 LYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNT 482
           LYVHSNRQFFRECDIYGT+DFIFGNAVVVFQSCNIYARKPM GQK TITAQDRKDPNQNT
Sbjct: 424 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKXTITAQDRKDPNQNT 483

Query: 483 GISIHACKIIATSDLQASKGSFPTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFA 542
           GISIHACKI+AT DL+ASKGSFPTFLGRPWKLYSRVVYMVS MGDHIHPRGWLEWQGSFA
Sbjct: 484 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 543

Query: 543 LDTLYYGEYSNSGPGAAVGQRVKWSGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 602
           LDTLYYGEY NSGPGAAVGQRVKW GYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA
Sbjct: 544 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 603

Query: 603 GLSAN 605
           GLS N
Sbjct: 604 GLSTN 605

BLAST of Bhi12G000559 vs. ExPASy TrEMBL
Match: A0A0A0KJG1 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G139430 PE=3 SV=1)

HSP 1 Score: 1070.5 bits (2767), Expect = 2.7e-309
Identity = 543/604 (89.90%), Postives = 568/604 (94.04%), Query Frame = 0

Query: 3   NNNYNKLHSSLSATSTPASSFHLSGNDLPPTPPPSSSHRRRSRILIISFLSVLLIAASIV 62
           NN+YNKLH+S SA +TPA+SF LSGND    PPPSSS RRRSRILIISF+SVLLIAAS+V
Sbjct: 4   NNSYNKLHTSHSANTTPAASFRLSGND---PPPPSSSLRRRSRILIISFMSVLLIAASVV 63

Query: 63  SAAVLVRSRIASNEPHFPHNKPSQAISRACSHTLYPSLCLSSLLSIPDALSADVHDLVHI 122
           SAA+LVRS + S++PHFPHNKPSQAISRACSHTLYPSLCLSSLLS P A SADVHDLVHI
Sbjct: 64  SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 123

Query: 123 SLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSVSLFSTEASNH 182
           SLNLTLQHLTKALYSTSQIP+ QIS DPLAHSAYEDCMELLNDA+DAFS+SLFS +ASNH
Sbjct: 124 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 183

Query: 183 DVMTWLSAALTYHDTCTDGFADVADLAVKNQVEAKLSDLSEMISNSLAIFSGFGGGDLPV 242
           D+MTWLSAALTYHDTCT GF DVADL VK++VEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 184 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 243

Query: 243 QNR-RRRLMESGTRSWAAENGGD-GGFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGKF 302
           +NR RRRLMES T SWAAENGGD  GFP WLS KDRRLLAAP+S IQADIVVAKDGSGKF
Sbjct: 244 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 303

Query: 303 KTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVF 362
           KTVAEAI+AAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV+
Sbjct: 304 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 363

Query: 363 DKVTTFRTATFAGSGTGIILRDMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTL 422
           DKVTTFRTATFAGSGT IILRDM FENTAGPSKHQAVALRLSADHAVVY CNIIGYQDTL
Sbjct: 364 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 423

Query: 423 YVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTG 482
           YVHSNRQFFRECDIYGT+DFIFGNAVVVFQSCNIYARKPM GQKNTITAQDRKDPNQNTG
Sbjct: 424 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 483

Query: 483 ISIHACKIIATSDLQASKGSFPTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFAL 542
           ISIHACKI+AT DL+ASKGSFPTFLGRPWKLYSRVVYMVS MGDHIHPRGWLEWQGSFAL
Sbjct: 484 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 543

Query: 543 DTLYYGEYSNSGPGAAVGQRVKWSGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 602
           DTLYYGEY NSGPGAAVGQRVKW GYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG
Sbjct: 544 DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 603

Query: 603 LSAN 605
           LS N
Sbjct: 604 LSTN 604

BLAST of Bhi12G000559 vs. ExPASy TrEMBL
Match: A0A6J1E2V1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430323 PE=3 SV=1)

HSP 1 Score: 1018.5 bits (2632), Expect = 1.2e-293
Identity = 526/605 (86.94%), Postives = 554/605 (91.57%), Query Frame = 0

Query: 2   INNNYNKLHSSLSATSTPASSFHLSGNDLPPTPPPSSSHRRRSRILIISFLSVLLIAASI 61
           +NNNYN LHSS S   TPASSF +S +   P PPPS S  RR RIL+ISF S+LLI AS 
Sbjct: 1   MNNNYNMLHSSHSG-DTPASSFRVSNDHSDPLPPPSPSSSRR-RILLISFFSILLITASA 60

Query: 62  VSAAVLVRSRI-ASNEPHFPHNKPSQAISRACSHTLYPSLCLSSLLSIPDALSADVHDLV 121
           VSA VLV SRI AS E HFPHNKP+QAISRACS TLYPSLCLSSLLS P +LSADVHDLV
Sbjct: 61  VSAVVLVHSRIAASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLV 120

Query: 122 HISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSVSLFSTEAS 181
           HISLNLTLQHLTKALYSTSQIPISQISNDPLA SAYEDCMELLNDAV+AFS+SLFST A+
Sbjct: 121 HISLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTAT 180

Query: 182 NHDVMTWLSAALTYHDTCTDGFADVADLAVKNQVEAKLSDLSEMISNSLAIFSGFGGGDL 241
           NHDVMTWLSAALTYHDTCTDGF+DVAD  +K+Q+ AKLSDLSEMISNSLAIFSGFGG DL
Sbjct: 181 NHDVMTWLSAALTYHDTCTDGFSDVAD-PIKDQITAKLSDLSEMISNSLAIFSGFGGDDL 240

Query: 242 PVQN-RRRRLMESGTRSWAAENGGDGGFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGK 301
           PV+N RRRRLMES  +  A EN GDG FP+W+S KDRRLLAAPIS+IQADIVVAKDGSGK
Sbjct: 241 PVENRRRRRLMESDEQWLAEENNGDGDFPRWVSGKDRRLLAAPISSIQADIVVAKDGSGK 300

Query: 302 FKTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV 361
           FKTVAEAI AAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV
Sbjct: 301 FKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV 360

Query: 362 FDKVTTFRTATFAGSGTGIILRDMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 421
           +DKVTTFRTATFAGSGTG+ILRDM FENTAGP+KHQAVALRLSADHAVV+R N+IGYQDT
Sbjct: 361 YDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQDT 420

Query: 422 LYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNT 481
           LYVHSNRQFFRECDIYGT+DFIFGNAVVVFQSCNIYARKPM GQKNTITAQDRKDPNQNT
Sbjct: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMSGQKNTITAQDRKDPNQNT 480

Query: 482 GISIHACKIIATSDLQASKGSFPTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFA 541
           GISIHACKIIAT DLQASKGSFPT+LGRPWKLYSRVVYMVS MGDHIHPRGWLEWQGSFA
Sbjct: 481 GISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540

Query: 542 LDTLYYGEYSNSGPGAAVGQRVKWSGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 601
           LDTLYYGEY NSGPGAAVGQRVKW GYRVITS +EA+KFTVGQFIYGSSWLPSTGVSFSA
Sbjct: 541 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSAIEATKFTVGQFIYGSSWLPSTGVSFSA 600

Query: 602 GLSAN 605
           GLS N
Sbjct: 601 GLSTN 602

BLAST of Bhi12G000559 vs. ExPASy TrEMBL
Match: A0A6J1IA52 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111471502 PE=3 SV=1)

HSP 1 Score: 1011.9 bits (2615), Expect = 1.1e-291
Identity = 522/607 (86.00%), Postives = 553/607 (91.10%), Query Frame = 0

Query: 2   INNNYNKLHSSLSATSTPASSFHLSG--NDLPPTPPPSSSHRRRSRILIISFLSVLLIAA 61
           +NNNYNKLHSS S   TP +SF +S   ++ PP PPPSSSHR   RIL+ISF S+LLI A
Sbjct: 1   MNNNYNKLHSSRSG-DTPVTSFRVSNDHSNPPPPPPPSSSHR---RILLISFFSILLITA 60

Query: 62  SIVSAAVLVRSRI-ASNEPHFPHNKPSQAISRACSHTLYPSLCLSSLLSIPDALSADVHD 121
           S VSA VLV SRI  S E HFPHNKP+QAISRACS TLYPSLC+SSLLS P +LSADVHD
Sbjct: 61  STVSAVVLVHSRITTSTESHFPHNKPTQAISRACSLTLYPSLCISSLLSAPGSLSADVHD 120

Query: 122 LVHISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSVSLFSTE 181
           LVHISLNLTLQHLTKALYSTSQIPISQISNDPLA SAYEDCMELLNDAV+AFS+SLFST 
Sbjct: 121 LVHISLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTT 180

Query: 182 ASNHDVMTWLSAALTYHDTCTDGFADVADLAVKNQVEAKLSDLSEMISNSLAIFSGFGGG 241
           A+NHDVMTWLSAALTYHDTCTDGF+DVAD  VK+Q+ AKLSDLSEMISNSLAIFSGFGGG
Sbjct: 181 ATNHDVMTWLSAALTYHDTCTDGFSDVAD-PVKDQITAKLSDLSEMISNSLAIFSGFGGG 240

Query: 242 DLPVQN-RRRRLMESGTRSWAAENGGDGGFPQWLSVKDRRLLAAPISAIQADIVVAKDGS 301
           DLPV+N RRRRLMES  +  A EN GDG FP+W+S KDRRLLAAPIS+IQADIVVAKDGS
Sbjct: 241 DLPVENRRRRRLMESDEQWLAEENNGDGDFPRWVSGKDRRLLAAPISSIQADIVVAKDGS 300

Query: 302 GKFKTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 361
           GKFKTVAEAI AAPSSS RRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK
Sbjct: 301 GKFKTVAEAIAAAPSSSSRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 360

Query: 362 SVFDKVTTFRTATFAGSGTGIILRDMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQ 421
           SV+DKVTTFRTATFAGSGTG+ILRDM FENTAGP+KHQAVALRLSADH VV+R N+IGYQ
Sbjct: 361 SVYDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHTVVFRSNVIGYQ 420

Query: 422 DTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQ 481
           DTLYVHSNRQFFRECDIYGT+DFIFGNA VVFQSCNIYARKPM GQKNTITAQDRKDPNQ
Sbjct: 421 DTLYVHSNRQFFRECDIYGTIDFIFGNAAVVFQSCNIYARKPMSGQKNTITAQDRKDPNQ 480

Query: 482 NTGISIHACKIIATSDLQASKGSFPTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGS 541
           NTGISIHACKIIAT DLQASKGSFPT+LGRPWKLYSRVVYMVS +GDHIHPRGWLEWQGS
Sbjct: 481 NTGISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSLGDHIHPRGWLEWQGS 540

Query: 542 FALDTLYYGEYSNSGPGAAVGQRVKWSGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSF 601
           FALDTLYYGEY NSGP AAVGQRVKW GYRVITS +EA+KFTVGQFIYGSSWLPSTGVSF
Sbjct: 541 FALDTLYYGEYMNSGPSAAVGQRVKWPGYRVITSAIEATKFTVGQFIYGSSWLPSTGVSF 600

Query: 602 SAGLSAN 605
           SAGLS N
Sbjct: 601 SAGLSTN 602

BLAST of Bhi12G000559 vs. ExPASy TrEMBL
Match: A0A6J1H2K1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459120 PE=3 SV=1)

HSP 1 Score: 986.9 bits (2550), Expect = 3.8e-284
Identity = 514/604 (85.10%), Postives = 548/604 (90.73%), Query Frame = 0

Query: 4   NNYNKLHSSLSATSTPASSFHLSGNDLPPTPPPSSSHRRRSRILIISFLSVLLIAASIVS 63
           NNYNKLHSS S   TPA +F +S     P PP SS+ RRR+RIL +S +S+LLI AS VS
Sbjct: 2   NNYNKLHSSASG-DTPA-TFQVSHT---PNPPSSSASRRRTRILFVSLISILLITASAVS 61

Query: 64  AAVLVRSRIASNEPHFPHNKPSQAISRACSHTLYPSLCLSSLLSIPDALSADVHDLVHIS 123
           AAVL+ S+      HFPHNKP+QAIS ACSHTLYPSLC SSLLS P +LSADVH LVHIS
Sbjct: 62  AAVLLHSQ----SSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHIS 121

Query: 124 LNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSVSLFSTEASNHD 183
           LNLTLQHLTKALY++SQIP+ QISN+PLAHSAY+DC+ELLNDAVDAFS+SLFST ASNHD
Sbjct: 122 LNLTLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHD 181

Query: 184 VMTWLSAALTYHDTCTDGFADVADLAVKNQVEAKLSDLSEMISNSLAIFSGFGGGDLPVQ 243
           +MTWLSAALTYHDTC+DGFADV D  VK+Q+ A+LSDLSEMISNSLAIFSG+G G LPVQ
Sbjct: 182 IMTWLSAALTYHDTCSDGFADVDD-PVKDQMAARLSDLSEMISNSLAIFSGYGEG-LPVQ 241

Query: 244 NRRRRLMESGTRSWAAENG---GDGGFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGKF 303
           N RRRLMES  R WAAENG    +G FP W+S KDRRLLAAPISAIQADIVVAKDGSGKF
Sbjct: 242 N-RRRLMES-DREWAAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKF 301

Query: 304 KTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVF 363
           KTVAEAI+AAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVF
Sbjct: 302 KTVAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVF 361

Query: 364 DKVTTFRTATFAGSGTGIILRDMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTL 423
           DKVTTFRTATFAGSGTGIILRDM FENTAGPSKHQAVALR+SADHAVVYRCNIIGYQDTL
Sbjct: 362 DKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRVSADHAVVYRCNIIGYQDTL 421

Query: 424 YVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTG 483
           YVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMP QKNTITAQ+RKDPNQNTG
Sbjct: 422 YVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQNTG 481

Query: 484 ISIHACKIIATSDLQASKGSFPTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFAL 543
           ISIHACKI+AT+DL+ASKGSFPTFLGRPWKLYSRVVYMVS MGDHIHPRGWLEWQGSFAL
Sbjct: 482 ISIHACKILATTDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 541

Query: 544 DTLYYGEYSNSGPGAAVGQRVKWSGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 603
           DTLYYGEY NSGPGAA GQRV W GYRVITST+EA+KFTVGQFIYGSSWLPSTGVSFSAG
Sbjct: 542 DTLYYGEYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSAG 592

Query: 604 LSAN 605
           LSAN
Sbjct: 602 LSAN 592

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G53370.13.7e-19961.96pectin methylesterase PCR fragment F [more]
AT3G49220.16.0e-19760.00Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G14310.12.4e-12944.18pectin methylesterase 3 [more]
AT1G53830.15.3e-12945.34pectin methylesterase 2 [more]
AT1G11580.11.5e-11842.69methylesterase PCR A [more]
Match NameE-valueIdentityDescription
Q9FK055.3e-19861.96Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3... [more]
Q9M3B08.4e-19660.00Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3... [more]
O048863.4e-12845.55Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1[more]
O490063.4e-12844.18Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME... [more]
P839483.4e-12845.20Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_038874841.10.0e+00100.00probable pectinesterase/pectinesterase inhibitor 61 [Benincasa hispida][more]
XP_008437596.10.0e+0091.07PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor... [more]
XP_011654619.15.6e-30989.90probable pectinesterase/pectinesterase inhibitor 61 [Cucumis sativus] >KGN49865.... [more]
KAG6579321.12.9e-29487.11putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosp... [more]
XP_022922302.12.4e-29386.94probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A1S3AUZ60.0e+0091.07Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482964 PE=3 SV=1[more]
A0A0A0KJG12.7e-30989.90Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G139430 PE=3 SV=1[more]
A0A6J1E2V11.2e-29386.94Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430323 PE=3 SV=1[more]
A0A6J1IA521.1e-29186.00Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111471502 PE=3 SV=1[more]
A0A6J1H2K13.8e-28485.10Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459120 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 83..231
e-value: 1.0E-38
score: 144.6
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 55..233
e-value: 8.3E-33
score: 111.2
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 87..231
e-value: 3.7E-31
score: 108.5
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 283..601
e-value: 1.2E-121
score: 408.0
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 83..235
e-value: 1.7E-36
score: 127.5
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 86..233
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 289..586
e-value: 1.4E-144
score: 480.3
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 39..601
NoneNo IPR availablePANTHERPTHR31707:SF184PECTINESTERASEcoord: 39..601
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 91..237
e-value: 8.76895E-36
score: 129.866
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 434..443
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 284..600

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi12M000559Bhi12M000559mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity