Bhi12G000528 (gene) Wax gourd (B227) v1

Overview


Name	Bhi12G000528
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	acidic endochitinase
Location	chr12: 18604902 .. 18605598 (+)
RNA-Seq Expression	Bhi12G000528
Synteny	Bhi12G000528

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTGGTCATTGCAACCCTAATAACAACGGTTGCAACCTTTTTGAGCGACAAAATAAATTCTTGCAAAAGTCAAGGCACCAAGTCCCCTCTATCGCGGCGGTGCAGGGAGCTATTCGCTCTCCTCGGCCGACGACGGGAGAGAAGTCACGAACTTCCTTTGGAAACAACTTTCTCTGTGGGCAGTCGAGTTCGAGGCCACTAGGCAATGCCGTTTTGGACGGCATTGATTTCGATATCGAATCCGGCTTCCTGGTCAGTTCTGGGACATTATTAGCTCAGGAGCTAAAAGAACTTCGGTCAAGTCTCTCTCCGCGCGCCGCAGTGTCCGATTCCCAACAGCACACCTAGACGCAGCCATTAAAACTGGTGTTGTTGATTTGTTTGGGTCAAATTCTACAACAACCCCACAATGCATGTTTGCAGATAACGGCGGACAATCCTCCTCAATTCTTGGAAACAATGGACGACGTTCTGTTTCGAGGCTTTTCATGGGGTTGCCGGCCGCGCCTGAGGCCGCCCCAAGCGGCGGCTTTATTCCGGCGGATGTGCTTATTTCTCATGTTCTTCCGACCATAAAAACTTCTTCCAACTATGGAGGAGTGATGTTAATGGAGCGCAAGGCGTTTGATAATGGCTACAGCGATGCCATTAAAGGCAGATCAATTAATTACTTATTAACAATTTTAAAGCCATGA

mRNA sequence

ATGCTGGTCATTGCAACCCTAATAACAACGGTTGCAACCTTTTTGAGCGACAAAATAAATTCTTGCAAAAGTCAAGGCACCAAGTCCCCTCTATCGCGGCGGTGCAGGGAGCTATTCGCTCTCCTCGGCCGACGACGGGAGAGAAGTCACGAACTTCCTTTGGAAACAACTTTCTCTTTCTGGGACATTATTAGCTCAGGAGCTAAAAGAACTTCGGTCAAGTCTCTCTCCGCGCGCCGCAGTGTCCGATTCCCAACAGCACACCTAGACGCAGCCATTAAAACTGGTGTTGTTGATTTGTTTGGGTCAAATTCTACAACAACCCCACAATGCATGTTTGCAGATAACGGCGGACAATCCTCCTCAATTCTTGGAAACAATGGACGACGTTCTGTTTCGAGGCTTTTCATGGGGTTGCCGGCCGCGCCTGAGGCCGCCCCAAGCGGCGGCTTTATTCCGGCGGATGTGCTTATTTCTCATGTTCTTCCGACCATAAAAACTTCTTCCAACTATGGAGGAGTGATGTTAATGGAGCGCAAGGCGTTTGATAATGGCTACAGCGATGCCATTAAAGGCAGATCAATTAATTACTTATTAACAATTTTAAAGCCATGA

Coding sequence (CDS)

ATGCTGGTCATTGCAACCCTAATAACAACGGTTGCAACCTTTTTGAGCGACAAAATAAATTCTTGCAAAAGTCAAGGCACCAAGTCCCCTCTATCGCGGCGGTGCAGGGAGCTATTCGCTCTCCTCGGCCGACGACGGGAGAGAAGTCACGAACTTCCTTTGGAAACAACTTTCTCTTTCTGGGACATTATTAGCTCAGGAGCTAAAAGAACTTCGGTCAAGTCTCTCTCCGCGCGCCGCAGTGTCCGATTCCCAACAGCACACCTAGACGCAGCCATTAAAACTGGTGTTGTTGATTTGTTTGGGTCAAATTCTACAACAACCCCACAATGCATGTTTGCAGATAACGGCGGACAATCCTCCTCAATTCTTGGAAACAATGGACGACGTTCTGTTTCGAGGCTTTTCATGGGGTTGCCGGCCGCGCCTGAGGCCGCCCCAAGCGGCGGCTTTATTCCGGCGGATGTGCTTATTTCTCATGTTCTTCCGACCATAAAAACTTCTTCCAACTATGGAGGAGTGATGTTAATGGAGCGCAAGGCGTTTGATAATGGCTACAGCGATGCCATTAAAGGCAGATCAATTAATTACTTATTAACAATTTTAAAGCCATGA

Protein sequence

MLVIATLITTVATFLSDKINSCKSQGTKSPLSRRCRELFALLGRRRERSHELPLETTFSFWDIISSGAKRTSVKSLSARRSVRFPTAHLDAAIKTGVVDLFGSNSTTTPQCMFADNGGQSSSILGNNGRRSVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTIKTSSNYGGVMLMERKAFDNGYSDAIKGRSINYLLTILKP

Homology

[Close]

BLAST of Bhi12G000528 vs. TAIR 10
Match: AT5G24090.1 (chitinase A )

HSP 1 Score: 71.6 bits (174), Expect = 8.1e-13
Identity = 45/110 (40.91%), Postives = 60/110 (54.55%), Query Frame = 0


Query: 84  FPTAHLDAAIKTGVVDLFGSNSTTTPQCMFADNGGQSSSILGNNGRRSVS--RLFMGLPA 143
           FP   + +A+ T   D         P C ++    Q+     N    S++  + F+GLPA
Sbjct: 190 FPDRLMGSALNTKRFDYVWIQFYNNPPCSYSSGNTQNLFDSWNKWTTSIAAQKFFLGLPA 249

Query: 144 APEAAPSGGFIPADVLISHVLPTIKTSSNYGGVMLMERKAFD-NGYSDAI 191
           APEAA S G+IP DVL S +LPT+K S  YGGVML  +   D NGYS +I
Sbjct: 250 APEAAGS-GYIPPDVLTSQILPTLKKSRKYGGVMLWSKFWDDKNGYSSSI 298

[Close]

BLAST of Bhi12G000528 vs. ExPASy Swiss-Prot
Match: P17541 (Acidic endochitinase OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 2.4e-33
Identity = 96/207 (46.38%), Postives = 106/207 (51.21%), Query Frame = 0


Query: 14  FLSDKINSCKSQGTKSPLS---------------RRCRELFALLGRRRERSHELPLETTF 73
           FLSD+INSCKSQ  K  LS                +    F        +S   PL    
Sbjct: 84  FLSDEINSCKSQNVKVLLSIGGGAGSYSLSSADDAKQVANFIWNSYLGGQSDSRPLGAAV 143

Query: 74  -------------SFWDIISSGAKRTSVKSLSARRSVRFPTAHLDAAIKTGVVDLFGSNS 133
                         FWD+++   K      LSA      P AHLDAAIKTG+ D      
Sbjct: 144 LDGVDFDIESGSGQFWDVLAQELKNFGQVILSAAPQCPIPDAHLDAAIKTGLFDSVWVQF 203

Query: 134 TTTPQCMFADNGGQSSSILGNNGRRSVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTI 193
              P CMFADN     S          S+L+MGLPAA EAAPSGGFIPADVLIS VLPTI
Sbjct: 204 YNNPPCMFADNADNLLSSWNQWTAFPTSKLYMGLPAAREAAPSGGFIPADVLISQVLPTI 263

[Close]

BLAST of Bhi12G000528 vs. ExPASy Swiss-Prot
Match: P36908 (Acidic endochitinase OS=Cicer arietinum OX=3827 PE=2 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.1e-19
Identity = 57/120 (47.50%), Postives = 75/120 (62.50%), Query Frame = 0


Query: 76  LSARRSVRFPTAHLDAAIKTGVVDLFGSNSTTTPQCMFADNGGQSSSILGNNGRRS--VS 135
           LSA     +P AHLD+AI+TG+ D         PQC ++ NG  ++ +   N   S    
Sbjct: 173 LSAAPQCPYPDAHLDSAIQTGLFDYVWVQFYNNPQCQYS-NGNINNLVNAWNQWTSSQAK 232

Query: 136 RLFMGLPAAPEAAPSGGFIPADVLISHVLPTIKTSSNYGGVMLMER-KAFDNGYSDAIKG 193
           ++F+G+PA+  AAPSGG IPADVL S VLP IKTS  YGGVM+ +R     +GYS+AIKG
Sbjct: 233 QVFLGVPASDAAAPSGGLIPADVLTSQVLPAIKTSPKYGGVMIWDRFNDAQSGYSNAIKG 291

[Close]

BLAST of Bhi12G000528 vs. ExPASy Swiss-Prot
Match: P29024 (Acidic endochitinase OS=Phaseolus angularis OX=3914 PE=2 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.2e-16
Identity = 60/141 (42.55%), Postives = 80/141 (56.74%), Query Frame = 0


Query: 57  TFSFWDIISSGAKR-TSVKSLSARRSVRFPTAHLDAAIKTGVVDLFGSNSTTTPQCMFAD 116
           T   WD ++   K   S   L+A      P AHLD AIKTG+ D+        P C +  
Sbjct: 159 TGEHWDDLARALKGFNSQLLLTAAPQCPIPDAHLDTAIKTGLFDIVWVQFYNNPPCQY-- 218

Query: 117 NGGQSSSILGNNGRRSVS---RLFMGLPAAPEAAPSGGFIPADVLISHVLPTIKTSSNYG 176
           + G ++ ++ +  + + S   +LF+G+PA+  AA S GFIPADVL S VLPTIK SS YG
Sbjct: 219 SSGNTNDLISSWNQWTSSQAKQLFLGVPASTAAAGS-GFIPADVLTSQVLPTIKGSSKYG 278

Query: 177 GVMLMER-KAFDNGYSDAIKG 193
           GVML +R     +GYS AI G
Sbjct: 279 GVMLWDRFNDGQSGYSGAIIG 296

[Close]

BLAST of Bhi12G000528 vs. ExPASy Swiss-Prot
Match: G1UH28 (Acidic endochitinase Pun g 14, amyloplastic OS=Punica granatum OX=22663 GN=PSC PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.3e-15
Identity = 54/143 (37.76%), Postives = 76/143 (53.15%), Query Frame = 0


Query: 57  TFSFWDIISSGAKRTSVKS----LSARRSVRFPTAHLDAAIKTGVVDLFGSNSTTTPQCM 116
           T +F+D ++      S ++    L+A      P +HLDAA+ TG+ D         P   
Sbjct: 156 TTTFYDTLARALSSRSTQAAKVYLTAAPQCPHPDSHLDAALNTGLFDNVWIQFYNNPLAQ 215

Query: 117 FADNGGQSSSILGN----NGRRSVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTIKTS 176
              + G ++ IL +        +  ++F+GLPAAPEAA S G+IP DVL   +LP IKTS
Sbjct: 216 CQYSSGNTNDILSSWNTWTSSTTAGKIFLGLPAAPEAAGS-GYIPPDVLTGQILPQIKTS 275

Query: 177 SNYGGVMLMERKAFDNGYSDAIK 192
           + YGGVML   K +D  YS  IK
Sbjct: 276 AKYGGVMLYS-KFYDTTYSTTIK 296

[Close]

BLAST of Bhi12G000528 vs. ExPASy Swiss-Prot
Match: P36910 (Acidic endochitinase SE2 OS=Beta vulgaris OX=161934 GN=SE2 PE=1 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.7e-15
Identity = 52/116 (44.83%), Postives = 64/116 (55.17%), Query Frame = 0


Query: 76  LSARRSVRFPTAHLDAAIKTGVVDLFGSNSTTTPQCMFADNGGQSSSILGNNGRRSVSRL 135
           LSA      P A L  AI TG+ D         P C +  +     S          +++
Sbjct: 177 LSAAPQCPLPDASLSTAIATGLFDYVWVQFYNNPPCQYDTSADNLLSSWNQWTTVQANQI 236

Query: 136 FMGLPAAPEAAPSGGFIPADVLISHVLPTIKTSSNYGGVMLMERKAFDNGYSDAIK 192
           F+GLPA+ +AA S GFIPAD L S VLPTIK S+ YGGVML   KA+D+GYS AIK
Sbjct: 237 FLGLPASTDAAGS-GFIPADALTSQVLPTIKGSAKYGGVMLWS-KAYDSGYSSAIK 290

[Close]

BLAST of Bhi12G000528 vs. NCBI nr
Match: XP_038875781.1 (LOW QUALITY PROTEIN: acidic endochitinase-like [Benincasa hispida])

HSP 1 Score: 166.8 bits (421), Expect = 2.0e-37
Identity = 112/212 (52.83%), Postives = 118/212 (55.66%), Query Frame = 0


Query: 13  TFLSDKINSCKSQGTKSPLS-------------RRCRELFALLGRR--RERSHELPLETT 72
           TFLSDKINSCKSQGTK  LS                RE+   L       +S   PL   
Sbjct: 83  TFLSDKINSCKSQGTKVLLSIGGGAGSYSLSSADDGREVTNFLWNNFLGGQSSSRPLGNA 142

Query: 73  F-------------SFWDIISSGAKRTSVKSLSARRSVRFPTAHLDAAIKTGVVDLFGSN 132
                          FWDI++   K      LSA      P AHLDAAIKTG+ D     
Sbjct: 143 VLDGIDFDIESGSGQFWDILAQELKNFGQVLLSAAPQCPIPNAHLDAAIKTGLFDFVWVQ 202

Query: 133 STTTPQCMFADNGGQSSSILGNNGRRSVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPT 192
               P CMFADN     +         VSRLFMGLPAAPEAAPSGGFIPADVLISHVLPT
Sbjct: 203 FYNNPPCMFADNADNLLNSWKQWTTFPVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPT 262

Query: 193 IKTSSNYGGVMLMERKAFDNGYSDAIKGRSIN 197
           IKTSSNYGGVML   KAFDNGYSDAIKG SIN
Sbjct: 263 IKTSSNYGGVMLWS-KAFDNGYSDAIKG-SIN 292

[Close]

BLAST of Bhi12G000528 vs. NCBI nr
Match: KAA0042574.1 (acidic endochitinase precursor [Cucumis melo var. makuwa] >TYK05978.1 acidic endochitinase precursor [Cucumis melo var. makuwa])

HSP 1 Score: 150.2 bits (378), Expect = 1.9e-32
Identity = 97/207 (46.86%), Postives = 115/207 (55.56%), Query Frame = 0


Query: 14  FLSDKINSCKSQGTKSPLS----------------RRCRELF--ALLGRRRE-------- 73
           FLSD+INSC+SQ  K  LS                R+       + LG + +        
Sbjct: 84  FLSDEINSCQSQNVKVLLSIGGGAGSYSLSSADDARQVANFLWNSYLGGQSDSRPLGAAV 143

Query: 74  -RSHELPLET-TFSFWDIISSGAKRTSVKSLSARRSVRFPTAHLDAAIKTGVVDLFGSNS 133
               +  +E+ +  FWD+++   K      LSA      P AHLDAA+KTG+ D      
Sbjct: 144 LNGIDFDIESGSGQFWDVLAQELKSFGQVILSAAPQCPIPDAHLDAAVKTGLFDSVWVQF 203

Query: 134 TTTPQCMFADNGGQSSSILGNNGRRSVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTI 193
              P CMFADN     S         +S+L+MGLPAAPEAAPSGGFIPADVLIS VLPTI
Sbjct: 204 YNNPPCMFADNADNLLSSWNQWTAFPISKLYMGLPAAPEAAPSGGFIPADVLISQVLPTI 263

[Close]

BLAST of Bhi12G000528 vs. NCBI nr
Match: NP_001315368.1 (acidic endochitinase precursor [Cucumis melo] >AAF64474.1 chitinase 1 [Cucumis melo])

HSP 1 Score: 150.2 bits (378), Expect = 1.9e-32
Identity = 97/207 (46.86%), Postives = 115/207 (55.56%), Query Frame = 0


Query: 14  FLSDKINSCKSQGTKSPLS----------------RRCRELF--ALLGRRRE-------- 73
           FLSD+INSC+SQ  K  LS                R+       + LG + +        
Sbjct: 84  FLSDEINSCQSQNVKVLLSIGGGAGSYSLSSADDARQVANFLWNSYLGGQSDSRPLGAAV 143

Query: 74  -RSHELPLET-TFSFWDIISSGAKRTSVKSLSARRSVRFPTAHLDAAIKTGVVDLFGSNS 133
               +  +E+ +  FWD+++   K      LSA      P AHLDAA+KTG+ D      
Sbjct: 144 LNGIDFDIESGSGQFWDVLAQELKSFGQVILSAAPQCPIPDAHLDAAVKTGLFDSVWVQF 203

Query: 134 TTTPQCMFADNGGQSSSILGNNGRRSVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTI 193
              P CMFADN     S         +S+L+MGLPAAPEAAPSGGFIPADVLIS VLPTI
Sbjct: 204 YNNPPCMFADNADNLLSSWNQWTAFPISKLYMGLPAAPEAAPSGGFIPADVLISQVLPTI 263

[Close]

BLAST of Bhi12G000528 vs. NCBI nr
Match: AAC37394.1 (ORF 1 [Cucumis sativus] >KAE8647809.1 hypothetical protein Csa_000394 [Cucumis sativus])

HSP 1 Score: 150.2 bits (378), Expect = 1.9e-32
Identity = 96/208 (46.15%), Postives = 112/208 (53.85%), Query Frame = 0


Query: 14  FLSDKINSCKSQGTKSPLS-------------RRCRELFALLGRR--RERSHELPLETTF 73
           F+SD+INSC+SQ  K  LS                +++   L       +S   PL    
Sbjct: 85  FVSDEINSCQSQNVKVLLSIGGGVGRYSLSSANNAKQVAGFLWNNYLGGQSDSRPLGDAV 144

Query: 74  -------------SFWDIISSGAKRTSVKSLSARRSVRFPTAHLDAAIKTGVVDLFGSNS 133
                         FWD+++   K      LSA     FP A LDAAI+TG+ D      
Sbjct: 145 LDGVDFVIGFGSGQFWDVLARELKSFGQVILSAAPQCPFPDAQLDAAIRTGLFDSVWVQF 204

Query: 134 TTTPQCMFADNGGQSSSILGNNGRRSVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTI 193
              P CM+ADN     S         +S+L+MGLPAAPEAAPSGGFIPADVLIS VLPTI
Sbjct: 205 YNNPPCMYADNADNLLSSWNQWAAYPISKLYMGLPAAPEAAPSGGFIPADVLISQVLPTI 264

[Close]

BLAST of Bhi12G000528 vs. NCBI nr
Match: AFK31317.1 (acidic class III chitinase [Citrullus colocynthis])

HSP 1 Score: 146.4 bits (368), Expect = 2.8e-31
Identity = 99/207 (47.83%), Postives = 110/207 (53.14%), Query Frame = 0


Query: 14  FLSDKINSCKSQGTKSPLS-------------RRCRELFALLGRR--RERSHELPL---- 73
           FLSD+INSCKSQG K  LS                R++   L       +S   PL    
Sbjct: 84  FLSDEINSCKSQGIKVLLSIGGGAGSYSLSSTDDARDVANFLWNNFLGGQSSSRPLGDAV 143

Query: 74  ---------ETTFSFWDIISSGAKRTSVKSLSARRSVRFPTAHLDAAIKTGVVDLFGSNS 133
                      +  +WD ++   K      LSA      P AHLDAAIKTG+ D      
Sbjct: 144 LDGIDFDIESGSGQWWDELARQLKGFGQVLLSAAPQCPIPDAHLDAAIKTGLFDFVWVQF 203

Query: 134 TTTPQCMFADNGGQSSSILGNNGRRSVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTI 193
              P CMFADN     +          + LFMGLPAAPEAAPSGGFIPADVLIS VLPTI
Sbjct: 204 YNNPPCMFADNADNLLNSWSQWTTFPAASLFMGLPAAPEAAPSGGFIPADVLISQVLPTI 263

[Close]

BLAST of Bhi12G000528 vs. ExPASy TrEMBL
Match: A0A0A0KM09 (Glyco_hydro_18 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139770 PE=4 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 5.4e-33
Identity = 96/208 (46.15%), Postives = 112/208 (53.85%), Query Frame = 0


Query: 14  FLSDKINSCKSQGTKSPLS-------------RRCRELFALLGRR--RERSHELPLETTF 73
           F+SD+INSC+SQ  K  LS                +++   L       +S   PL    
Sbjct: 85  FVSDEINSCQSQNVKVLLSIGGGVGRYSLSSANNAKQVAGFLWNNYLGGQSDSRPLGDAV 144

Query: 74  -------------SFWDIISSGAKRTSVKSLSARRSVRFPTAHLDAAIKTGVVDLFGSNS 133
                         FWD+++   K      LSA      P AHLDAAI+TG+ D      
Sbjct: 145 LDGVDFVIGFGSGQFWDVLARELKSFGQVILSAAPQCPIPDAHLDAAIRTGLFDSVWVQF 204

Query: 134 TTTPQCMFADNGGQSSSILGNNGRRSVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTI 193
              P CM+ADN     S         +S+L+MGLPAAPEAAPSGGFIPADVLIS VLPTI
Sbjct: 205 YNNPPCMYADNADNLLSSWNQWAAYPISKLYMGLPAAPEAAPSGGFIPADVLISQVLPTI 264

[Close]

BLAST of Bhi12G000528 vs. ExPASy TrEMBL
Match: A0A1S3AUF6 (acidic endochitinase OS=Cucumis melo OX=3656 GN=LOC103482936 PE=3 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 5.4e-33
Identity = 97/207 (46.86%), Postives = 115/207 (55.56%), Query Frame = 0


Query: 14  FLSDKINSCKSQGTKSPLS----------------RRCRELF--ALLGRRRE-------- 73
           FLSD+INSC+SQ  K  LS                R+       + LG + +        
Sbjct: 84  FLSDEINSCQSQNVKVLLSIGGGAGSYSLSSADDARQVANFLWNSYLGGQSDSRPLGAAV 143

Query: 74  -RSHELPLET-TFSFWDIISSGAKRTSVKSLSARRSVRFPTAHLDAAIKTGVVDLFGSNS 133
               +  +E+ +  FWD+++   K      LSA      P AHLDAA+KTG+ D      
Sbjct: 144 LNGIDFDIESGSGQFWDVLAQELKSFGQVILSAAPQCPIPDAHLDAAVKTGLFDFVWVQF 203

Query: 134 TTTPQCMFADNGGQSSSILGNNGRRSVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTI 193
              P CMFADN     S         +S+L+MGLPAAPEAAPSGGFIPADVLIS VLPTI
Sbjct: 204 YNNPPCMFADNADNLLSSWNQWTAFPISKLYMGLPAAPEAAPSGGFIPADVLISQVLPTI 263

[Close]

BLAST of Bhi12G000528 vs. ExPASy TrEMBL
Match: A0A5D3C438 (Acidic endochitinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00740 PE=3 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 9.3e-33
Identity = 97/207 (46.86%), Postives = 115/207 (55.56%), Query Frame = 0


Query: 14  FLSDKINSCKSQGTKSPLS----------------RRCRELF--ALLGRRRE-------- 73
           FLSD+INSC+SQ  K  LS                R+       + LG + +        
Sbjct: 84  FLSDEINSCQSQNVKVLLSIGGGAGSYSLSSADDARQVANFLWNSYLGGQSDSRPLGAAV 143

Query: 74  -RSHELPLET-TFSFWDIISSGAKRTSVKSLSARRSVRFPTAHLDAAIKTGVVDLFGSNS 133
               +  +E+ +  FWD+++   K      LSA      P AHLDAA+KTG+ D      
Sbjct: 144 LNGIDFDIESGSGQFWDVLAQELKSFGQVILSAAPQCPIPDAHLDAAVKTGLFDSVWVQF 203

Query: 134 TTTPQCMFADNGGQSSSILGNNGRRSVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTI 193
              P CMFADN     S         +S+L+MGLPAAPEAAPSGGFIPADVLIS VLPTI
Sbjct: 204 YNNPPCMFADNADNLLSSWNQWTAFPISKLYMGLPAAPEAAPSGGFIPADVLISQVLPTI 263

[Close]

BLAST of Bhi12G000528 vs. ExPASy TrEMBL
Match: Q39656 (ORF 1 OS=Cucumis sativus OX=3659 PE=4 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 9.3e-33
Identity = 96/208 (46.15%), Postives = 112/208 (53.85%), Query Frame = 0


Query: 14  FLSDKINSCKSQGTKSPLS-------------RRCRELFALLGRR--RERSHELPLETTF 73
           F+SD+INSC+SQ  K  LS                +++   L       +S   PL    
Sbjct: 85  FVSDEINSCQSQNVKVLLSIGGGVGRYSLSSANNAKQVAGFLWNNYLGGQSDSRPLGDAV 144

Query: 74  -------------SFWDIISSGAKRTSVKSLSARRSVRFPTAHLDAAIKTGVVDLFGSNS 133
                         FWD+++   K      LSA     FP A LDAAI+TG+ D      
Sbjct: 145 LDGVDFVIGFGSGQFWDVLARELKSFGQVILSAAPQCPFPDAQLDAAIRTGLFDSVWVQF 204

Query: 134 TTTPQCMFADNGGQSSSILGNNGRRSVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTI 193
              P CM+ADN     S         +S+L+MGLPAAPEAAPSGGFIPADVLIS VLPTI
Sbjct: 205 YNNPPCMYADNADNLLSSWNQWAAYPISKLYMGLPAAPEAAPSGGFIPADVLISQVLPTI 264

[Close]

BLAST of Bhi12G000528 vs. ExPASy TrEMBL
Match: Q9M544 (Chitinase 1 OS=Cucumis melo OX=3656 PE=2 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 9.3e-33
Identity = 97/207 (46.86%), Postives = 115/207 (55.56%), Query Frame = 0


Query: 14  FLSDKINSCKSQGTKSPLS----------------RRCRELF--ALLGRRRE-------- 73
           FLSD+INSC+SQ  K  LS                R+       + LG + +        
Sbjct: 84  FLSDEINSCQSQNVKVLLSIGGGAGSYSLSSADDARQVANFLWNSYLGGQSDSRPLGAAV 143

Query: 74  -RSHELPLET-TFSFWDIISSGAKRTSVKSLSARRSVRFPTAHLDAAIKTGVVDLFGSNS 133
               +  +E+ +  FWD+++   K      LSA      P AHLDAA+KTG+ D      
Sbjct: 144 LNGIDFDIESGSGQFWDVLAQELKSFGQVILSAAPQCPIPDAHLDAAVKTGLFDSVWVQF 203

Query: 134 TTTPQCMFADNGGQSSSILGNNGRRSVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTI 193
              P CMFADN     S         +S+L+MGLPAAPEAAPSGGFIPADVLIS VLPTI
Sbjct: 204 YNNPPCMFADNADNLLSSWNQWTAFPISKLYMGLPAAPEAAPSGGFIPADVLISQVLPTI 263

The following BLAST results are available for this feature:

BLAST of Bhi12G000528 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G24090.1	8.1e-13	40.91	chitinase A	[more]

BLAST of Bhi12G000528 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17541	2.4e-33	46.38	Acidic endochitinase OS=Cucumis sativus OX=3659 PE=1 SV=1	[more]
P36908	1.1e-19	47.50	Acidic endochitinase OS=Cicer arietinum OX=3827 PE=2 SV=1	[more]
P29024	1.2e-16	42.55	Acidic endochitinase OS=Phaseolus angularis OX=3914 PE=2 SV=1	[more]
G1UH28	1.3e-15	37.76	Acidic endochitinase Pun g 14, amyloplastic OS=Punica granatum OX=22663 GN=PSC P...	[more]
P36910	1.7e-15	44.83	Acidic endochitinase SE2 OS=Beta vulgaris OX=161934 GN=SE2 PE=1 SV=1	[more]

BLAST of Bhi12G000528 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038875781.1	2.0e-37	52.83	LOW QUALITY PROTEIN: acidic endochitinase-like [Benincasa hispida]	[more]
KAA0042574.1	1.9e-32	46.86	acidic endochitinase precursor [Cucumis melo var. makuwa] >TYK05978.1 acidic end...	[more]
NP_001315368.1	1.9e-32	46.86	acidic endochitinase precursor [Cucumis melo] >AAF64474.1 chitinase 1 [Cucumis m...	[more]
AAC37394.1	1.9e-32	46.15	ORF 1 [Cucumis sativus] >KAE8647809.1 hypothetical protein Csa_000394 [Cucumis s...	[more]
AFK31317.1	2.8e-31	47.83	acidic class III chitinase [Citrullus colocynthis]	[more]

BLAST of Bhi12G000528 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KM09	5.4e-33	46.15	Glyco_hydro_18 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139...	[more]
A0A1S3AUF6	5.4e-33	46.86	acidic endochitinase OS=Cucumis melo OX=3656 GN=LOC103482936 PE=3 SV=1	[more]
A0A5D3C438	9.3e-33	46.86	Acidic endochitinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376...	[more]
Q39656	9.3e-33	46.15	ORF 1 OS=Cucumis sativus OX=3659 PE=4 SV=1	[more]
Q9M544	9.3e-33	46.86	Chitinase 1 OS=Cucumis melo OX=3656 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 57..193 e-value: 4.1E-13 score: 51.2
None	No IPR available	PANTHER	PTHR45708:SF22	ACIDIC ENDOCHITINASE	coord: 75..192
None	No IPR available	PANTHER	PTHR45708	ENDOCHITINASE	coord: 75..192
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 82..177

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi12M000528	Bhi12M000528	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071704	organic substance metabolic process
molecular_function	GO:0016798	hydrolase activity, acting on glycosyl bonds