Bhi12G000527 (gene) Wax gourd (B227) v1

Overview


Name	Bhi12G000527
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Acidic endochitinase
Location	chr12: 18591507 .. 18592315 (+)
RNA-Seq Expression	Bhi12G000527
Synteny	Bhi12G000527

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTATCGGAATTGCCATCTACTGGGGCCAATACGGCAACGAAGGCTCTCTTGCCTCTACTTGCGCCACTAGAATCTACGAGTTTGTCAACATAGCATTTCTCTCGTCCTTTGGCAACGGTCAAACTCCGATCCTCAACCATGCTGGTCATTGCAACCCTAATAACAACGGTTGCACCTTTTTGAGCGACAAAATAAATTCTTGCAAAAGTCAAGGCACCAAAGTCCTCCTCTCTATCGGCGGCGGTGCAGGGAGCTATTCGCTCTCCTCGGCCGACGACGGGAGAGAAGTCACGAACTTCCTTTGGAACAACTTTCTCGGTGGGCAGTCGAGTTCGAGGCCACTAGGCAATGCCGTTTTGGACGGCATTGATTTCGATATCGAATCCGGCTCTGGTCAGTTCTGGGACATATTAGCTCAGGAGCTAAAGAACTTCGGTCAAGTCCTTCTCTCCGCGGCGCCGCAGTGTCCGATCCCCAACGCACACCTAGACGCAGCCATTAAAACTGGTTTGTTTGATTTTGTTTGGGTTCAATTCTACAACAACCCACCATGCATGTTTGCAGATAACGCGGACAATCTCCTCAATTCTTGGAAACAATGGACGACGTTTCCTGTTTCGAGGCTTTTCATGGGGTTGCCGGCCGCGCCTGAGGCCGCCCCAAGCGGCGGCTTTATTCCGGCGGATGTGCTTATTTCTCATGTTCTTCCGACCATAAAAACTTCTTCCAACTATGGAGGAGTGATGTTATGGAGCAAGGCGTTTGATAATGGCTACAGCGATGCCATTAAAGGCAGCATCAATTAA

mRNA sequence

ATGCTATCGGAATTGCCATCTACTGGGGCCAATACGGCAACGAAGGCTCTCTTGCCTCTACTTGCGCCACTAGAATCTACGAGCACCAAAGTCCTCCTCTCTATCGGCGGCGGTGCAGGGAGCTATTCGCTCTCCTCGGCCGACGACGGGAGAGAAGTCACGAACTTCCTTTGGAACAACTTTCTCGGTGGGCAGTCGAGTTCGAGGCCACTAGGCAATGCCGTTTTGGACGGCATTGATTTCGATATCGAATCCGGCTCTGGTCAGTTCTGGGACATATTAGCTCAGGAGCTAAAGAACTTCGGTCAAGTCCTTCTCTCCGCGGCGCCGCAGTGTCCGATCCCCAACGCACACCTAGACGCAGCCATTAAAACTGGTTTGTTTGATTTTGTTTGGGTTCAATTCTACAACAACCCACCATGCATGTTTGCAGATAACGCGGACAATCTCCTCAATTCTTGGAAACAATGGACGACGTTTCCTGTTTCGAGGCTTTTCATGGGGTTGCCGGCCGCGCCTGAGGCCGCCCCAAGCGGCGGCTTTATTCCGGCGGATGTGCTTATTTCTCATGTTCTTCCGACCATAAAAACTTCTTCCAACTATGGAGGAGTGATGTTATGGAGCAAGGCGTTTGATAATGGCTACAGCGATGCCATTAAAGGCAGCATCAATTAA

Coding sequence (CDS)

ATGCTATCGGAATTGCCATCTACTGGGGCCAATACGGCAACGAAGGCTCTCTTGCCTCTACTTGCGCCACTAGAATCTACGAGCACCAAAGTCCTCCTCTCTATCGGCGGCGGTGCAGGGAGCTATTCGCTCTCCTCGGCCGACGACGGGAGAGAAGTCACGAACTTCCTTTGGAACAACTTTCTCGGTGGGCAGTCGAGTTCGAGGCCACTAGGCAATGCCGTTTTGGACGGCATTGATTTCGATATCGAATCCGGCTCTGGTCAGTTCTGGGACATATTAGCTCAGGAGCTAAAGAACTTCGGTCAAGTCCTTCTCTCCGCGGCGCCGCAGTGTCCGATCCCCAACGCACACCTAGACGCAGCCATTAAAACTGGTTTGTTTGATTTTGTTTGGGTTCAATTCTACAACAACCCACCATGCATGTTTGCAGATAACGCGGACAATCTCCTCAATTCTTGGAAACAATGGACGACGTTTCCTGTTTCGAGGCTTTTCATGGGGTTGCCGGCCGCGCCTGAGGCCGCCCCAAGCGGCGGCTTTATTCCGGCGGATGTGCTTATTTCTCATGTTCTTCCGACCATAAAAACTTCTTCCAACTATGGAGGAGTGATGTTATGGAGCAAGGCGTTTGATAATGGCTACAGCGATGCCATTAAAGGCAGCATCAATTAA

Protein sequence

MLSELPSTGANTATKALLPLLAPLESTSTKVLLSIGGGAGSYSLSSADDGREVTNFLWNNFLGGQSSSRPLGNAVLDGIDFDIESGSGQFWDILAQELKNFGQVLLSAAPQCPIPNAHLDAAIKTGLFDFVWVQFYNNPPCMFADNADNLLNSWKQWTTFPVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTIKTSSNYGGVMLWSKAFDNGYSDAIKGSIN

Homology

[Close]

BLAST of Bhi12G000527 vs. TAIR 10
Match: AT5G24090.1 (chitinase A )

HSP 1 Score: 239.6 bits (610), Expect = 2.5e-63
Identity = 115/207 (55.56%), Postives = 150/207 (72.46%), Query Frame = 0


Query: 25  ESTSTKVLLSIGGGAGSYSLSSADDGREVTNFLWNNFLGGQSSSRPLGNAVLDGIDFDIE 84
           +S   KV+LS+GGG G+YS+ S +D + + ++LWNNFLGG+SSSRPLG+AVLDGIDF+IE
Sbjct: 97  QSRGIKVMLSLGGGIGNYSIGSREDAKVIADYLWNNFLGGKSSSRPLGDAVLDGIDFNIE 156

Query: 85  SGSGQFWDILAQELKNFG----QVLLSAAPQCPIPNAHLDAAIKTGLFDFVWVQFYNNPP 144
            GS Q WD LA+ L  F     ++ L+ APQCP P+  + +A+ T  FD+VW+QFYNNPP
Sbjct: 157 LGSPQHWDDLARTLSKFSHRGRKIYLTGAPQCPFPDRLMGSALNTKRFDYVWIQFYNNPP 216

Query: 145 CMFAD-NADNLLNSWKQWTT-FPVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTIKTS 204
           C ++  N  NL +SW +WTT     + F+GLPAAPEAA S G+IP DVL S +LPT+K S
Sbjct: 217 CSYSSGNTQNLFDSWNKWTTSIAAQKFFLGLPAAPEAAGS-GYIPPDVLTSQILPTLKKS 276

Query: 205 SNYGGVMLWSKAFD--NGYSDAIKGSI 224
             YGGVMLWSK +D  NGYS +I  S+
Sbjct: 277 RKYGGVMLWSKFWDDKNGYSSSILASV 302

[Close]

BLAST of Bhi12G000527 vs. ExPASy Swiss-Prot
Match: P17541 (Acidic endochitinase OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 1.1e-100
Identity = 171/199 (85.93%), Postives = 186/199 (93.47%), Query Frame = 0


Query: 25  ESTSTKVLLSIGGGAGSYSLSSADDGREVTNFLWNNFLGGQSSSRPLGNAVLDGIDFDIE 84
           +S + KVLLSIGGGAGSYSLSSADD ++V NF+WN++LGGQS SRPLG AVLDG+DFDIE
Sbjct: 93  KSQNVKVLLSIGGGAGSYSLSSADDAKQVANFIWNSYLGGQSDSRPLGAAVLDGVDFDIE 152

Query: 85  SGSGQFWDILAQELKNFGQVLLSAAPQCPIPNAHLDAAIKTGLFDFVWVQFYNNPPCMFA 144
           SGSGQFWD+LAQELKNFGQV+LSAAPQCPIP+AHLDAAIKTGLFD VWVQFYNNPPCMFA
Sbjct: 153 SGSGQFWDVLAQELKNFGQVILSAAPQCPIPDAHLDAAIKTGLFDSVWVQFYNNPPCMFA 212

Query: 145 DNADNLLNSWKQWTTFPVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTIKTSSNYGGV 204
           DNADNLL+SW QWT FP S+L+MGLPAA EAAPSGGFIPADVLIS VLPTIK SSNYGGV
Sbjct: 213 DNADNLLSSWNQWTAFPTSKLYMGLPAAREAAPSGGFIPADVLISQVLPTIKASSNYGGV 272

Query: 205 MLWSKAFDNGYSDAIKGSI 224
           MLWSKAFDNGYSD+IKGSI
Sbjct: 273 MLWSKAFDNGYSDSIKGSI 291

[Close]

BLAST of Bhi12G000527 vs. ExPASy Swiss-Prot
Match: P36910 (Acidic endochitinase SE2 OS=Beta vulgaris OX=161934 GN=SE2 PE=1 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 3.0e-74
Identity = 139/222 (62.61%), Postives = 167/222 (75.23%), Query Frame = 0


Query: 6   PSTGANTATKALLPLLAPLESTSTKVLLSIGGGAGSYSLSSADDGREVTNFLWNNFLGGQ 65
           P+T  N+ +      +   +    KVLLSIGGGAG YSLSS DD     ++LWN +LGGQ
Sbjct: 77  PATNCNSLSSD----IKTCQQAGIKVLLSIGGGAGGYSLSSTDDANTFADYLWNTYLGGQ 136

Query: 66  SSSRPLGNAVLDGIDFDIESGSGQFWDILAQEL--KNFGQ--VLLSAAPQCPIPNAHLDA 125
           SS+RPLG+AVLDGIDFDIESG G+FWD LA+ L   N GQ  V LSAAPQCP+P+A L  
Sbjct: 137 SSTRPLGDAVLDGIDFDIESGDGRFWDDLARALAGHNNGQKTVYLSAAPQCPLPDASLST 196

Query: 126 AIKTGLFDFVWVQFYNNPPCMFADNADNLLNSWKQWTTFPVSRLFMGLPAAPEAAPSGGF 185
           AI TGLFD+VWVQFYNNPPC +  +ADNLL+SW QWTT   +++F+GLPA+ +AA S GF
Sbjct: 197 AIATGLFDYVWVQFYNNPPCQYDTSADNLLSSWNQWTTVQANQIFLGLPASTDAAGS-GF 256

Query: 186 IPADVLISHVLPTIKTSSNYGGVMLWSKAFDNGYSDAIKGSI 224
           IPAD L S VLPTIK S+ YGGVMLWSKA+D+GYS AIK S+
Sbjct: 257 IPADALTSQVLPTIKGSAKYGGVMLWSKAYDSGYSSAIKSSV 293

[Close]

BLAST of Bhi12G000527 vs. ExPASy Swiss-Prot
Match: P29024 (Acidic endochitinase OS=Phaseolus angularis OX=3914 PE=2 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 2.0e-73
Identity = 134/203 (66.01%), Postives = 164/203 (80.79%), Query Frame = 0


Query: 25  ESTSTKVLLSIGGGAGSYSLSSADDGREVTNFLWNNFLGGQSSSRPLGNAVLDGIDFDIE 84
           +S   KVLLS+GG +GSYSL+SADD  +V N++WNNFLGGQSSSRPLG+A+LDG+DFDIE
Sbjct: 97  QSKDIKVLLSLGGASGSYSLTSADDATQVANYIWNNFLGGQSSSRPLGDAILDGVDFDIE 156

Query: 85  SGSGQFWDILAQELKNF-GQVLLSAAPQCPIPNAHLDAAIKTGLFDFVWVQFYNNPPCMF 144
           SG+G+ WD LA+ LK F  Q+LL+AAPQCPIP+AHLD AIKTGLFD VWVQFYNNPPC +
Sbjct: 157 SGTGEHWDDLARALKGFNSQLLLTAAPQCPIPDAHLDTAIKTGLFDIVWVQFYNNPPCQY 216

Query: 145 AD-NADNLLNSWKQWTTFPVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTIKTSSNYG 204
           +  N ++L++SW QWT+    +LF+G+PA+  AA S GFIPADVL S VLPTIK SS YG
Sbjct: 217 SSGNTNDLISSWNQWTSSQAKQLFLGVPASTAAAGS-GFIPADVLTSQVLPTIKGSSKYG 276

Query: 205 GVMLWSKAFD--NGYSDAIKGSI 224
           GVMLW +  D  +GYS AI GS+
Sbjct: 277 GVMLWDRFNDGQSGYSGAIIGSV 298

[Close]

BLAST of Bhi12G000527 vs. ExPASy Swiss-Prot
Match: P36908 (Acidic endochitinase OS=Cicer arietinum OX=3827 PE=2 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 5.3e-71
Identity = 137/223 (61.43%), Postives = 171/223 (76.68%), Query Frame = 0


Query: 6   PSTGANTATKALLPLLAPLESTSTKVLLSIGGGAGSYSLSSADDGREVTNFLWNNFLGGQ 65
           PST  N  TK   P +   ++   KVLLS+GGGAGSYSL+SA++   + N+LWNNFLGG 
Sbjct: 75  PST--NGCTK-FSPEIQACQAKGIKVLLSLGGGAGSYSLNSAEEATTLANYLWNNFLGGT 134

Query: 66  SSSRPLGNAVLDGIDFDIESGSGQFWDILAQELKNFGQ--VLLSAAPQCPIPNAHLDAAI 125
           S+SRPLG+AVLDGIDFDIESG GQ +D LA+ L  F Q  V LSAAPQCP P+AHLD+AI
Sbjct: 135 STSRPLGDAVLDGIDFDIESG-GQHYDELAKALNGFSQQKVYLSAAPQCPYPDAHLDSAI 194

Query: 126 KTGLFDFVWVQFYNNPPCMFAD-NADNLLNSWKQWTTFPVSRLFMGLPAAPEAAPSGGFI 185
           +TGLFD+VWVQFYNNP C +++ N +NL+N+W QWT+    ++F+G+PA+  AAPSGG I
Sbjct: 195 QTGLFDYVWVQFYNNPQCQYSNGNINNLVNAWNQWTSSQAKQVFLGVPASDAAAPSGGLI 254

Query: 186 PADVLISHVLPTIKTSSNYGGVMLWSKAFD--NGYSDAIKGSI 224
           PADVL S VLP IKTS  YGGVM+W +  D  +GYS+AIKGS+
Sbjct: 255 PADVLTSQVLPAIKTSPKYGGVMIWDRFNDAQSGYSNAIKGSV 293

[Close]

BLAST of Bhi12G000527 vs. ExPASy Swiss-Prot
Match: P29060 (Acidic endochitinase OS=Nicotiana tabacum OX=4097 PE=1 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 5.9e-70
Identity = 132/222 (59.46%), Postives = 161/222 (72.52%), Query Frame = 0


Query: 6   PSTGANTATKALLPLLAPLESTSTKVLLSIGGGAGSYSLSSADDGREVTNFLWNNFLGGQ 65
           P+ GA T    L   +   ++   KV+LS+GGGAGSY LSSADD R V N+LWNN+LGGQ
Sbjct: 74  PNAGACT---GLSNDIRACQNQGIKVMLSLGGGAGSYFLSSADDARNVANYLWNNYLGGQ 133

Query: 66  SSSRPLGNAVLDGIDFDIESGSGQFWDILAQELKNFGQ---VLLSAAPQCPIPNAHLDAA 125
           S++RPLG+AVLDGIDFDIE G+ Q WD LA+ L  F Q   V L+AAPQCP P+  L+ A
Sbjct: 134 SNTRPLGDAVLDGIDFDIEGGTTQHWDELAKTLSQFSQQRKVYLTAAPQCPFPDTWLNGA 193

Query: 126 IKTGLFDFVWVQFYNNPPCMFA-DNADNLLNSWKQWTTFPVSRLFMGLPAAPEAAPSGGF 185
           + TGLFD+VWVQFYNNPPC ++  +ADNL N W QW      ++F+GLPAA  AA S GF
Sbjct: 194 LSTGLFDYVWVQFYNNPPCQYSGGSADNLKNYWNQWNAIQAGKIFLGLPAAQGAAGS-GF 253

Query: 186 IPADVLISHVLPTIKTSSNYGGVMLWSKAFDNGYSDAIKGSI 224
           IP+DVL+S VLP I  S  YGGVMLWSK +DNGYS AIK ++
Sbjct: 254 IPSDVLVSQVLPLINGSPKYGGVMLWSKFYDNGYSSAIKANV 291

[Close]

BLAST of Bhi12G000527 vs. NCBI nr
Match: XP_038875781.1 (LOW QUALITY PROTEIN: acidic endochitinase-like [Benincasa hispida])

HSP 1 Score: 412.1 bits (1058), Expect = 3.0e-111
Identity = 197/200 (98.50%), Postives = 198/200 (99.00%), Query Frame = 0


Query: 25  ESTSTKVLLSIGGGAGSYSLSSADDGREVTNFLWNNFLGGQSSSRPLGNAVLDGIDFDIE 84
           +S  TKVLLSIGGGAGSYSLSSADDGREVTNFLWNNFLGGQSSSRPLGNAVLDGIDFDIE
Sbjct: 93  KSQGTKVLLSIGGGAGSYSLSSADDGREVTNFLWNNFLGGQSSSRPLGNAVLDGIDFDIE 152

Query: 85  SGSGQFWDILAQELKNFGQVLLSAAPQCPIPNAHLDAAIKTGLFDFVWVQFYNNPPCMFA 144
           SGSGQFWDILAQELKNFGQVLLSAAPQCPIPNAHLDAAIKTGLFDFVWVQFYNNPPCMFA
Sbjct: 153 SGSGQFWDILAQELKNFGQVLLSAAPQCPIPNAHLDAAIKTGLFDFVWVQFYNNPPCMFA 212

Query: 145 DNADNLLNSWKQWTTFPVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTIKTSSNYGGV 204
           DNADNLLNSWKQWTTFPVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTIKTSSNYGGV
Sbjct: 213 DNADNLLNSWKQWTTFPVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTIKTSSNYGGV 272

Query: 205 MLWSKAFDNGYSDAIKGSIN 225
           MLWSKAFDNGYSDAIKGSIN
Sbjct: 273 MLWSKAFDNGYSDAIKGSIN 292

[Close]

BLAST of Bhi12G000527 vs. NCBI nr
Match: AFK31317.1 (acidic class III chitinase [Citrullus colocynthis])

HSP 1 Score: 377.1 bits (967), Expect = 1.1e-100
Identity = 179/199 (89.95%), Postives = 187/199 (93.97%), Query Frame = 0


Query: 25  ESTSTKVLLSIGGGAGSYSLSSADDGREVTNFLWNNFLGGQSSSRPLGNAVLDGIDFDIE 84
           +S   KVLLSIGGGAGSYSLSS DD R+V NFLWNNFLGGQSSSRPLG+AVLDGIDFDIE
Sbjct: 93  KSQGIKVLLSIGGGAGSYSLSSTDDARDVANFLWNNFLGGQSSSRPLGDAVLDGIDFDIE 152

Query: 85  SGSGQFWDILAQELKNFGQVLLSAAPQCPIPNAHLDAAIKTGLFDFVWVQFYNNPPCMFA 144
           SGSGQ+WD LA++LK FGQVLLSAAPQCPIP+AHLDAAIKTGLFDFVWVQFYNNPPCMFA
Sbjct: 153 SGSGQWWDELARQLKGFGQVLLSAAPQCPIPDAHLDAAIKTGLFDFVWVQFYNNPPCMFA 212

Query: 145 DNADNLLNSWKQWTTFPVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTIKTSSNYGGV 204
           DNADNLLNSW QWTTFP + LFMGLPAAPEAAPSGGFIPADVLIS VLPTIKTSSNYGGV
Sbjct: 213 DNADNLLNSWSQWTTFPAASLFMGLPAAPEAAPSGGFIPADVLISQVLPTIKTSSNYGGV 272

Query: 205 MLWSKAFDNGYSDAIKGSI 224
           MLWSKAFDNGYSDAIKGSI
Sbjct: 273 MLWSKAFDNGYSDAIKGSI 291

[Close]

BLAST of Bhi12G000527 vs. NCBI nr
Match: ABA26457.1 (acidic class III chitinase [Citrullus lanatus])

HSP 1 Score: 375.2 bits (962), Expect = 4.0e-100
Identity = 178/199 (89.45%), Postives = 188/199 (94.47%), Query Frame = 0


Query: 25  ESTSTKVLLSIGGGAGSYSLSSADDGREVTNFLWNNFLGGQSSSRPLGNAVLDGIDFDIE 84
           +S   KVLLSIGGGAGSYSLSSA+D R+V NFLWNNFLGGQSSSRPLG+AVLDGIDFDIE
Sbjct: 93  KSLGIKVLLSIGGGAGSYSLSSAEDARDVANFLWNNFLGGQSSSRPLGDAVLDGIDFDIE 152

Query: 85  SGSGQFWDILAQELKNFGQVLLSAAPQCPIPNAHLDAAIKTGLFDFVWVQFYNNPPCMFA 144
           SGSGQ+WD LA++LK FGQVLLSAAPQCPIP+AHLDAAIKTGLFDFVWVQFYNNPPCMFA
Sbjct: 153 SGSGQWWDELARQLKGFGQVLLSAAPQCPIPDAHLDAAIKTGLFDFVWVQFYNNPPCMFA 212

Query: 145 DNADNLLNSWKQWTTFPVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTIKTSSNYGGV 204
           DNADNLLNSW QWTTFP + LFMGLPAAPEAAPSGGFIPADVLIS VLPTIKTSSNYGGV
Sbjct: 213 DNADNLLNSWSQWTTFPAASLFMGLPAAPEAAPSGGFIPADVLISQVLPTIKTSSNYGGV 272

Query: 205 MLWSKAFDNGYSDAIKGSI 224
           MLWSKAFD+GYSDAIKGSI
Sbjct: 273 MLWSKAFDSGYSDAIKGSI 291

[Close]

BLAST of Bhi12G000527 vs. NCBI nr
Match: KAA0042574.1 (acidic endochitinase precursor [Cucumis melo var. makuwa] >TYK05978.1 acidic endochitinase precursor [Cucumis melo var. makuwa])

HSP 1 Score: 373.6 bits (958), Expect = 1.2e-99
Identity = 174/199 (87.44%), Postives = 189/199 (94.97%), Query Frame = 0


Query: 25  ESTSTKVLLSIGGGAGSYSLSSADDGREVTNFLWNNFLGGQSSSRPLGNAVLDGIDFDIE 84
           +S + KVLLSIGGGAGSYSLSSADD R+V NFLWN++LGGQS SRPLG AVL+GIDFDIE
Sbjct: 93  QSQNVKVLLSIGGGAGSYSLSSADDARQVANFLWNSYLGGQSDSRPLGAAVLNGIDFDIE 152

Query: 85  SGSGQFWDILAQELKNFGQVLLSAAPQCPIPNAHLDAAIKTGLFDFVWVQFYNNPPCMFA 144
           SGSGQFWD+LAQELK+FGQV+LSAAPQCPIP+AHLDAA+KTGLFD VWVQFYNNPPCMFA
Sbjct: 153 SGSGQFWDVLAQELKSFGQVILSAAPQCPIPDAHLDAAVKTGLFDSVWVQFYNNPPCMFA 212

Query: 145 DNADNLLNSWKQWTTFPVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTIKTSSNYGGV 204
           DNADNLL+SW QWT FP+S+L+MGLPAAPEAAPSGGFIPADVLIS VLPTIKTSSNYGGV
Sbjct: 213 DNADNLLSSWNQWTAFPISKLYMGLPAAPEAAPSGGFIPADVLISQVLPTIKTSSNYGGV 272

Query: 205 MLWSKAFDNGYSDAIKGSI 224
           MLWSKAFDNGYSDAIKGSI
Sbjct: 273 MLWSKAFDNGYSDAIKGSI 291

[Close]

BLAST of Bhi12G000527 vs. NCBI nr
Match: NP_001315368.1 (acidic endochitinase precursor [Cucumis melo] >AAF64474.1 chitinase 1 [Cucumis melo])

HSP 1 Score: 373.6 bits (958), Expect = 1.2e-99
Identity = 174/199 (87.44%), Postives = 189/199 (94.97%), Query Frame = 0


Query: 25  ESTSTKVLLSIGGGAGSYSLSSADDGREVTNFLWNNFLGGQSSSRPLGNAVLDGIDFDIE 84
           +S + KVLLSIGGGAGSYSLSSADD R+V NFLWN++LGGQS SRPLG AVL+GIDFDIE
Sbjct: 93  QSQNVKVLLSIGGGAGSYSLSSADDARQVANFLWNSYLGGQSDSRPLGAAVLNGIDFDIE 152

Query: 85  SGSGQFWDILAQELKNFGQVLLSAAPQCPIPNAHLDAAIKTGLFDFVWVQFYNNPPCMFA 144
           SGSGQFWD+LAQELK+FGQV+LSAAPQCPIP+AHLDAA+KTGLFD VWVQFYNNPPCMFA
Sbjct: 153 SGSGQFWDVLAQELKSFGQVILSAAPQCPIPDAHLDAAVKTGLFDSVWVQFYNNPPCMFA 212

Query: 145 DNADNLLNSWKQWTTFPVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTIKTSSNYGGV 204
           DNADNLL+SW QWT FP+S+L+MGLPAAPEAAPSGGFIPADVLIS VLPTIKTSSNYGGV
Sbjct: 213 DNADNLLSSWNQWTAFPISKLYMGLPAAPEAAPSGGFIPADVLISQVLPTIKTSSNYGGV 272

Query: 205 MLWSKAFDNGYSDAIKGSI 224
           MLWSKAFDNGYSDAIKGSI
Sbjct: 273 MLWSKAFDNGYSDAIKGSI 291

[Close]

BLAST of Bhi12G000527 vs. ExPASy TrEMBL
Match: I3RVA1 (Acidic class III chitinase OS=Citrullus colocynthis OX=252529 PE=3 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 5.1e-101
Identity = 179/199 (89.95%), Postives = 187/199 (93.97%), Query Frame = 0


Query: 25  ESTSTKVLLSIGGGAGSYSLSSADDGREVTNFLWNNFLGGQSSSRPLGNAVLDGIDFDIE 84
           +S   KVLLSIGGGAGSYSLSS DD R+V NFLWNNFLGGQSSSRPLG+AVLDGIDFDIE
Sbjct: 93  KSQGIKVLLSIGGGAGSYSLSSTDDARDVANFLWNNFLGGQSSSRPLGDAVLDGIDFDIE 152

Query: 85  SGSGQFWDILAQELKNFGQVLLSAAPQCPIPNAHLDAAIKTGLFDFVWVQFYNNPPCMFA 144
           SGSGQ+WD LA++LK FGQVLLSAAPQCPIP+AHLDAAIKTGLFDFVWVQFYNNPPCMFA
Sbjct: 153 SGSGQWWDELARQLKGFGQVLLSAAPQCPIPDAHLDAAIKTGLFDFVWVQFYNNPPCMFA 212

Query: 145 DNADNLLNSWKQWTTFPVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTIKTSSNYGGV 204
           DNADNLLNSW QWTTFP + LFMGLPAAPEAAPSGGFIPADVLIS VLPTIKTSSNYGGV
Sbjct: 213 DNADNLLNSWSQWTTFPAASLFMGLPAAPEAAPSGGFIPADVLISQVLPTIKTSSNYGGV 272

Query: 205 MLWSKAFDNGYSDAIKGSI 224
           MLWSKAFDNGYSDAIKGSI
Sbjct: 273 MLWSKAFDNGYSDAIKGSI 291

[Close]

BLAST of Bhi12G000527 vs. ExPASy TrEMBL
Match: A0A1S3AUF6 (acidic endochitinase OS=Cucumis melo OX=3656 GN=LOC103482936 PE=3 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 6.7e-101
Identity = 175/199 (87.94%), Postives = 190/199 (95.48%), Query Frame = 0


Query: 25  ESTSTKVLLSIGGGAGSYSLSSADDGREVTNFLWNNFLGGQSSSRPLGNAVLDGIDFDIE 84
           +S + KVLLSIGGGAGSYSLSSADD R+V NFLWN++LGGQS SRPLG AVL+GIDFDIE
Sbjct: 93  QSQNVKVLLSIGGGAGSYSLSSADDARQVANFLWNSYLGGQSDSRPLGAAVLNGIDFDIE 152

Query: 85  SGSGQFWDILAQELKNFGQVLLSAAPQCPIPNAHLDAAIKTGLFDFVWVQFYNNPPCMFA 144
           SGSGQFWD+LAQELK+FGQV+LSAAPQCPIP+AHLDAA+KTGLFDFVWVQFYNNPPCMFA
Sbjct: 153 SGSGQFWDVLAQELKSFGQVILSAAPQCPIPDAHLDAAVKTGLFDFVWVQFYNNPPCMFA 212

Query: 145 DNADNLLNSWKQWTTFPVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTIKTSSNYGGV 204
           DNADNLL+SW QWT FP+S+L+MGLPAAPEAAPSGGFIPADVLIS VLPTIKTSSNYGGV
Sbjct: 213 DNADNLLSSWNQWTAFPISKLYMGLPAAPEAAPSGGFIPADVLISQVLPTIKTSSNYGGV 272

Query: 205 MLWSKAFDNGYSDAIKGSI 224
           MLWSKAFDNGYSDAIKGSI
Sbjct: 273 MLWSKAFDNGYSDAIKGSI 291

[Close]

BLAST of Bhi12G000527 vs. ExPASy TrEMBL
Match: Q09Y38 (Acidic class III chitinase OS=Citrullus lanatus OX=3654 PE=2 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 1.9e-100
Identity = 178/199 (89.45%), Postives = 188/199 (94.47%), Query Frame = 0


Query: 25  ESTSTKVLLSIGGGAGSYSLSSADDGREVTNFLWNNFLGGQSSSRPLGNAVLDGIDFDIE 84
           +S   KVLLSIGGGAGSYSLSSA+D R+V NFLWNNFLGGQSSSRPLG+AVLDGIDFDIE
Sbjct: 93  KSLGIKVLLSIGGGAGSYSLSSAEDARDVANFLWNNFLGGQSSSRPLGDAVLDGIDFDIE 152

Query: 85  SGSGQFWDILAQELKNFGQVLLSAAPQCPIPNAHLDAAIKTGLFDFVWVQFYNNPPCMFA 144
           SGSGQ+WD LA++LK FGQVLLSAAPQCPIP+AHLDAAIKTGLFDFVWVQFYNNPPCMFA
Sbjct: 153 SGSGQWWDELARQLKGFGQVLLSAAPQCPIPDAHLDAAIKTGLFDFVWVQFYNNPPCMFA 212

Query: 145 DNADNLLNSWKQWTTFPVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTIKTSSNYGGV 204
           DNADNLLNSW QWTTFP + LFMGLPAAPEAAPSGGFIPADVLIS VLPTIKTSSNYGGV
Sbjct: 213 DNADNLLNSWSQWTTFPAASLFMGLPAAPEAAPSGGFIPADVLISQVLPTIKTSSNYGGV 272

Query: 205 MLWSKAFDNGYSDAIKGSI 224
           MLWSKAFD+GYSDAIKGSI
Sbjct: 273 MLWSKAFDSGYSDAIKGSI 291

[Close]

BLAST of Bhi12G000527 vs. ExPASy TrEMBL
Match: A0A5D3C438 (Acidic endochitinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00740 PE=3 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 5.7e-100
Identity = 174/199 (87.44%), Postives = 189/199 (94.97%), Query Frame = 0


Query: 25  ESTSTKVLLSIGGGAGSYSLSSADDGREVTNFLWNNFLGGQSSSRPLGNAVLDGIDFDIE 84
           +S + KVLLSIGGGAGSYSLSSADD R+V NFLWN++LGGQS SRPLG AVL+GIDFDIE
Sbjct: 93  QSQNVKVLLSIGGGAGSYSLSSADDARQVANFLWNSYLGGQSDSRPLGAAVLNGIDFDIE 152

Query: 85  SGSGQFWDILAQELKNFGQVLLSAAPQCPIPNAHLDAAIKTGLFDFVWVQFYNNPPCMFA 144
           SGSGQFWD+LAQELK+FGQV+LSAAPQCPIP+AHLDAA+KTGLFD VWVQFYNNPPCMFA
Sbjct: 153 SGSGQFWDVLAQELKSFGQVILSAAPQCPIPDAHLDAAVKTGLFDSVWVQFYNNPPCMFA 212

Query: 145 DNADNLLNSWKQWTTFPVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTIKTSSNYGGV 204
           DNADNLL+SW QWT FP+S+L+MGLPAAPEAAPSGGFIPADVLIS VLPTIKTSSNYGGV
Sbjct: 213 DNADNLLSSWNQWTAFPISKLYMGLPAAPEAAPSGGFIPADVLISQVLPTIKTSSNYGGV 272

Query: 205 MLWSKAFDNGYSDAIKGSI 224
           MLWSKAFDNGYSDAIKGSI
Sbjct: 273 MLWSKAFDNGYSDAIKGSI 291

[Close]

BLAST of Bhi12G000527 vs. ExPASy TrEMBL
Match: Q9M544 (Chitinase 1 OS=Cucumis melo OX=3656 PE=2 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 5.7e-100
Identity = 174/199 (87.44%), Postives = 189/199 (94.97%), Query Frame = 0


Query: 25  ESTSTKVLLSIGGGAGSYSLSSADDGREVTNFLWNNFLGGQSSSRPLGNAVLDGIDFDIE 84
           +S + KVLLSIGGGAGSYSLSSADD R+V NFLWN++LGGQS SRPLG AVL+GIDFDIE
Sbjct: 93  QSQNVKVLLSIGGGAGSYSLSSADDARQVANFLWNSYLGGQSDSRPLGAAVLNGIDFDIE 152

Query: 85  SGSGQFWDILAQELKNFGQVLLSAAPQCPIPNAHLDAAIKTGLFDFVWVQFYNNPPCMFA 144
           SGSGQFWD+LAQELK+FGQV+LSAAPQCPIP+AHLDAA+KTGLFD VWVQFYNNPPCMFA
Sbjct: 153 SGSGQFWDVLAQELKSFGQVILSAAPQCPIPDAHLDAAVKTGLFDSVWVQFYNNPPCMFA 212

Query: 145 DNADNLLNSWKQWTTFPVSRLFMGLPAAPEAAPSGGFIPADVLISHVLPTIKTSSNYGGV 204
           DNADNLL+SW QWT FP+S+L+MGLPAAPEAAPSGGFIPADVLIS VLPTIKTSSNYGGV
Sbjct: 213 DNADNLLSSWNQWTAFPISKLYMGLPAAPEAAPSGGFIPADVLISQVLPTIKTSSNYGGV 272

Query: 205 MLWSKAFDNGYSDAIKGSI 224
           MLWSKAFDNGYSDAIKGSI
Sbjct: 273 MLWSKAFDNGYSDAIKGSI 291

The following BLAST results are available for this feature:

BLAST of Bhi12G000527 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G24090.1	2.5e-63	55.56	chitinase A	[more]

BLAST of Bhi12G000527 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17541	1.1e-100	85.93	Acidic endochitinase OS=Cucumis sativus OX=3659 PE=1 SV=1	[more]
P36910	3.0e-74	62.61	Acidic endochitinase SE2 OS=Beta vulgaris OX=161934 GN=SE2 PE=1 SV=1	[more]
P29024	2.0e-73	66.01	Acidic endochitinase OS=Phaseolus angularis OX=3914 PE=2 SV=1	[more]
P36908	5.3e-71	61.43	Acidic endochitinase OS=Cicer arietinum OX=3827 PE=2 SV=1	[more]
P29060	5.9e-70	59.46	Acidic endochitinase OS=Nicotiana tabacum OX=4097 PE=1 SV=1	[more]

BLAST of Bhi12G000527 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038875781.1	3.0e-111	98.50	LOW QUALITY PROTEIN: acidic endochitinase-like [Benincasa hispida]	[more]
AFK31317.1	1.1e-100	89.95	acidic class III chitinase [Citrullus colocynthis]	[more]
ABA26457.1	4.0e-100	89.45	acidic class III chitinase [Citrullus lanatus]	[more]
KAA0042574.1	1.2e-99	87.44	acidic endochitinase precursor [Cucumis melo var. makuwa] >TYK05978.1 acidic end...	[more]
NP_001315368.1	1.2e-99	87.44	acidic endochitinase precursor [Cucumis melo] >AAF64474.1 chitinase 1 [Cucumis m...	[more]

BLAST of Bhi12G000527 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
I3RVA1	5.1e-101	89.95	Acidic class III chitinase OS=Citrullus colocynthis OX=252529 PE=3 SV=1	[more]
A0A1S3AUF6	6.7e-101	87.94	acidic endochitinase OS=Cucumis melo OX=3656 GN=LOC103482936 PE=3 SV=1	[more]
Q09Y38	1.9e-100	89.45	Acidic class III chitinase OS=Citrullus lanatus OX=3654 PE=2 SV=1	[more]
A0A5D3C438	5.7e-100	87.44	Acidic endochitinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376...	[more]
Q9M544	5.7e-100	87.44	Chitinase 1 OS=Cucumis melo OX=3656 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 8..223 e-value: 2.5E-68 score: 232.3
None	No IPR available	PANTHER	PTHR45708:SF22	ACIDIC ENDOCHITINASE	coord: 26..222
None	No IPR available	PANTHER	PTHR45708	ENDOCHITINASE	coord: 26..222
IPR001223	Glycoside hydrolase family 18, catalytic domain	PFAM	PF00704	Glyco_hydro_18	coord: 25..208 e-value: 1.4E-17 score: 64.4
IPR001223	Glycoside hydrolase family 18, catalytic domain	PROSITE	PS51910	GH18_2	coord: 1..224 score: 29.410486
IPR001579	Glycosyl hydrolases family 18 (GH18) active site	PROSITE	PS01095	GH18_1	coord: 76..84
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 23..213

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi12M000527	Bhi12M000527	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006032	chitin catabolic process
biological_process	GO:0000272	polysaccharide catabolic process
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0005615	extracellular space
molecular_function	GO:0004568	chitinase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds